FvH4_4g19840

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015931
Physical Location & Seq
Reverse (-)
23346283 .. 23349141
2859 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g19840.t1

Sequence Viewer

Length: 1047 bp
ATGATGTTCAAGTTACAAGGTTTAGTTGCCTTCGCAATCACATTCTTCGTCTCCGCCTCTTTGACTCATGGCTTTCCTCACGGTAGCCACTCCCCCATCCCAGAGCCAAAGGTCGACTCTCCCCTCCTGACTGAAAAGCTCAACACCAACAGAACCATCTTGGTCGATATCGCCGGCGATGGCCAATTCAAATCCATCCAGGCCGCCATCGATTCTGTTCCTGCAGGGAACAATCAGTGGGTTATCATTCATGTTAGGCAAGGGGTGTACAGAGAAAAAGTGCACATACCCCCCGAAAAATCCCATATTTTCATGAGAGGCAATGGGAAGGGAAGGACACAGATTGTTTGGTCCCAAAGCTCTGAGGTCAACGAGGAGTCTGCAACTTTCATAGTAGAAGCTCCATTATTCATTGCCTATGGGATCAGCTTCAAGAATGAGGCTCCAACTGGTGTGGCATACACATCGCAAAATCAGTCAGTGGCGGCGTTAGTGGGTGCAGACAAGGCTGCATTTTACCACTGTGCTTTCTACAGCACCCATAACACCCTCTTCGATTACAAGGGCAAGCATTACTATGATAACTGCTACATCCAAGGCTCAATCGACTTCATCTTCGGACGTGCTAAATCTATATACCATAACTGTGAGATCTTTGTGATTGGAGACAAGAGAGTTTCCATCAAAGGGTCTGTAACAGCTAATCACCGACAGAGTGCAGACGAGTGCAGTGGGTTTGTGTTCATCAAAGGCAAGGTGTATGGCGTAGGTGATCATGTCTACTTGGGTAGAGCCAAGGGTGCCTTTTCGAGAGTCGTTTATGCAGAAACCTATCTCTCTAGGACCATTATGCCCGAGGGCTGGACCAATTGGAGCTATGCCGGAGGAACAGAGAACCTGTACCACGCCGAGTACAACTGCAAGGGGCCAGGAGCCGAGTTTAGTGATCGCGTTCCATGGGCGAAGCAACTCACGGATCAAGAGGTTCAACCATTCCTGACGATCGACTTCATCGAAGGAAAGGATTGGCTGCCGGTGTCGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

349

Amino Acids

38.76

Weight (kDa)

6.89

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 54 - 340 7.4e-64 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015931)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17060
fragaria_vesca FvH4_4g19840
malus_domestica MD13G1153000.v1.1 MD16G1153400.v1.1
prunus_persica Prupe.1G116600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0425411
rosa_laevigata RLG00000007371
rosa_multiflora Rmu_sc0001900.1_g000046
rosa_roxburghii Rroxscaffold_5G00367370
rosa_rugosa Rorug04G0202700
rosa_samantha Rh4AG259900 Rh4BG265800 Rh4CG277100 Rh4DG261800
rosa_wichuraiana Rw4G022500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 800
AccI GTMKAC 2 cut(s) 114, 780
AccII CGCG 1 cut(s) 951
AciI CCGC 3 cut(s) 54, 204, 485
AclWI GGATC 2 cut(s) 431, 984
AcoI YGGCCR 1 cut(s) 181
AfaI GTAC 3 cut(s) 269, 902, 914
AfiI CCNNNNNNNGG 2 cut(s) 687, 861
AgsI TTSAA 4 cut(s) 10, 190, 433, 989
AjiI CACGTC 1 cut(s) 623
AjnI CCWGG 2 cut(s) 198, 928
AluBI AGCT 6 cut(s) 139, 360, 401, 429, 701, 876
AluI AGCT 6 cut(s) 139, 360, 401, 429, 701, 876
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 2 cut(s) 55, 660
Alw44I GTGCAC 1 cut(s) 281
AlwI GGATC 2 cut(s) 431, 984
Ama87I CYCGRG 1 cut(s) 854
AoxI GGCC 3 cut(s) 181, 201, 926
ApaLI GTGCAC 1 cut(s) 281
ApeKI GCWGC 2 cut(s) 509, 1030
ArsI GACNNNNNNTTYG 2 cut(s) 599, 631
AspS9I GGNCC 4 cut(s) 351, 843, 864, 926
AsuHPI GGTGA 2 cut(s) 698, 782
AvaI CYCGRG 1 cut(s) 854
AvaII GGWCC 3 cut(s) 351, 843, 864
BaeGI GKGCMC 1 cut(s) 285
BalI TGGCCA 1 cut(s) 183
BanI GGYRCC 1 cut(s) 800
Bbv12I GWGCWC 1 cut(s) 285
BbvI GCAGC 2 cut(s) 496, 1017
BccI CCATC 6 cut(s) 104, 164, 173, 203, 215, 689
BcgI CGANNNNNNTGC 4 cut(s) 362, 396, 447, 481
BciT130I CCWGG 2 cut(s) 200, 930
BclI TGATCA 1 cut(s) 772
BcoDI GTCTC 2 cut(s) 55, 660
BfaI CTAG 1 cut(s) 840
BfmI CTRYAG 2 cut(s) 222, 532
BglII AGATCT 1 cut(s) 651
BisI GCNGC 4 cut(s) 204, 486, 510, 1031
BlsI GCNGC 4 cut(s) 205, 487, 511, 1032
Bme1390I CCNGG 2 cut(s) 200, 930
Bme18I GGWCC 3 cut(s) 351, 843, 864
BmeT110I CYCGRG 1 cut(s) 854
BmgBI CACGTC 1 cut(s) 623
BmgT120I GGNCC 4 cut(s) 351, 843, 864, 926
BmiI GGNNCC 5 cut(s) 353, 444, 802, 927, 934
BmrFI CCNGG 2 cut(s) 200, 930
Bsa29I ATCGAT 1 cut(s) 210
BsaJI CCNNGG 4 cut(s) 595, 795, 855, 956
Bsc4I CCNNNNNNNGG 2 cut(s) 687, 861
Bse118I RCCGGY 2 cut(s) 173, 1033
Bse1I ACTGG 1 cut(s) 454
Bse3DI GCAATG 2 cut(s) 328, 411
BseBI CCWGG 2 cut(s) 200, 930
BseCI ATCGAT 1 cut(s) 210
BseDI CCNNGG 4 cut(s) 595, 795, 855, 956
BseGI GGATG 3 cut(s) 96, 195, 591
BseLI CCNNNNNNNGG 2 cut(s) 687, 861
BseMI GCAATG 2 cut(s) 328, 411
BseMII CTCAG 1 cut(s) 354
BseNI ACTGG 1 cut(s) 454
BseRI GAGGAG 1 cut(s) 389
BseSI GKGCMC 1 cut(s) 285
BseXI GCAGC 2 cut(s) 496, 1017
BsgI GTGCAG 3 cut(s) 519, 738, 748
Bsh1236I CGCG 1 cut(s) 951
Bsh1285I CGRYCG 1 cut(s) 1005
BshFI GGCC 3 cut(s) 183, 203, 928
BshNI GGYRCC 1 cut(s) 800
BshVI ATCGAT 1 cut(s) 210
BsiEI CGRYCG 1 cut(s) 1005
BsiHKAI GWGCWC 1 cut(s) 285
BsiHKCI CYCGRG 1 cut(s) 854
BsiSI CCGG 3 cut(s) 174, 882, 1034
BslFI GGGAC 1 cut(s) 337
BslI CCNNNNNNNGG 2 cut(s) 687, 861
BsmAI GTCTC 2 cut(s) 55, 660
BsmBI CGTCTC 1 cut(s) 55
BsmFI GGGAC 1 cut(s) 337
BsnI GGCC 3 cut(s) 183, 203, 928
BsoBI CYCGRG 1 cut(s) 854
Bsp1286I GDGCHC 1 cut(s) 285
Bsp1407I TGTACA 1 cut(s) 267
Bsp143I GATC 6 cut(s) 423, 651, 772, 946, 976, 1002
Bsp19I CCATGG 1 cut(s) 956
BspACI CCGC 3 cut(s) 54, 204, 485
BspANI GGCC 3 cut(s) 183, 203, 928
BspCNI CTCAG 1 cut(s) 355
BspDI ATCGAT 1 cut(s) 210
BspFNI CGCG 1 cut(s) 951
BspHI TCATGA 1 cut(s) 312
BspLI GGNNCC 5 cut(s) 353, 444, 802, 927, 934
BspMAI CTGCAG 1 cut(s) 226
BspPI GGATC 2 cut(s) 431, 984
BspT107I GGYRCC 1 cut(s) 800
BsrDI GCAATG 2 cut(s) 328, 411
BsrFI RCCGGY 2 cut(s) 173, 1033
BsrGI TGTACA 1 cut(s) 267
BsrI ACTGG 1 cut(s) 454
BssAI RCCGGY 2 cut(s) 173, 1033
BssECI CCNNGG 4 cut(s) 595, 795, 855, 956
BssMI GATC 6 cut(s) 423, 651, 772, 946, 976, 1002
BssT1I CCWWGG 3 cut(s) 595, 795, 956
Bst2UI CCWGG 2 cut(s) 200, 930
Bst4CI ACNGT 3 cut(s) 83, 524, 647
Bst6I CTCTTC 1 cut(s) 557
BstAUI TGTACA 1 cut(s) 267
BstC8I GCNNGC 2 cut(s) 175, 569
BstDEI CTNAG 1 cut(s) 363
BstDSI CCRYGG 1 cut(s) 956
BstF5I GGATG 3 cut(s) 96, 195, 591
BstFNI CGCG 1 cut(s) 951
BstKTI GATC 6 cut(s) 426, 654, 775, 949, 979, 1005
BstMAI GTCTC 2 cut(s) 55, 660
BstMBI GATC 6 cut(s) 423, 651, 772, 946, 976, 1002
BstMCI CGRYCG 1 cut(s) 1005
BstMWI GCNNNNNNNGC 2 cut(s) 506, 800
BstNI CCWGG 2 cut(s) 200, 930
BstSCI CCNGG 2 cut(s) 198, 928
BstSFI CTRYAG 2 cut(s) 222, 532
BstSLI GKGCMC 1 cut(s) 285
BstUI CGCG 1 cut(s) 951
BstV1I GCAGC 2 cut(s) 496, 1017
BstX2I RGATCY 1 cut(s) 651
BstYI RGATCY 1 cut(s) 651
Bsu15I ATCGAT 1 cut(s) 210
BsuRI GGCC 3 cut(s) 183, 203, 928
BsuTUI ATCGAT 1 cut(s) 210
BtgI CCRYGG 1 cut(s) 956
BtgZI GCGATG 2 cut(s) 192, 450
BtrI CACGTC 1 cut(s) 623
BtsCI GGATG 3 cut(s) 96, 195, 591
BtsI GCAGTG 1 cut(s) 736
BtsIMutI CAGTG 4 cut(s) 242, 486, 520, 736
Cac8I GCNNGC 2 cut(s) 175, 569
CciI TCATGA 1 cut(s) 312
Cfr10I RCCGGY 2 cut(s) 173, 1033
Cfr13I GGNCC 4 cut(s) 351, 843, 864, 926
ClaI ATCGAT 1 cut(s) 210
Csp6I GTAC 3 cut(s) 268, 901, 913
CspCI CAANNNNNGTGG 2 cut(s) 435, 470
CviAII CATG 5 cut(s) 68, 251, 313, 776, 957
CviQI GTAC 3 cut(s) 268, 901, 913
DdeI CTNAG 1 cut(s) 363
DpnI GATC 6 cut(s) 425, 653, 774, 948, 978, 1004
DpnII GATC 6 cut(s) 423, 651, 772, 946, 976, 1002
EaeI YGGCCR 1 cut(s) 181
Eam1104I CTCTTC 1 cut(s) 557
EarI CTCTTC 1 cut(s) 557
EciI GGCGGA 1 cut(s) 43
Eco130I CCWWGG 3 cut(s) 595, 795, 956
Eco32I GATATC 1 cut(s) 169
Eco47I GGWCC 3 cut(s) 351, 843, 864
Eco88I CYCGRG 1 cut(s) 854
EcoRII CCWGG 2 cut(s) 198, 928
EcoRV GATATC 1 cut(s) 169
EcoT14I CCWWGG 3 cut(s) 595, 795, 956
ErhI CCWWGG 3 cut(s) 595, 795, 956
Esp3I CGTCTC 1 cut(s) 55
FaeI CATG 5 cut(s) 71, 254, 316, 779, 960
FalI AAGNNNNNCTT 2 cut(s) 788, 820
FaqI GGGAC 1 cut(s) 337
FatI CATG 5 cut(s) 67, 250, 312, 775, 956
FbaI TGATCA 1 cut(s) 772
FblI GTMKAC 2 cut(s) 114, 780
Fnu4HI GCNGC 4 cut(s) 204, 486, 510, 1031
FokI GGATG 3 cut(s) 83, 182, 578
Fsp4HI GCNGC 4 cut(s) 204, 486, 510, 1031
FspBI CTAG 1 cut(s) 840
GluI GCNGC 4 cut(s) 204, 486, 510, 1031
HaeIII GGCC 3 cut(s) 183, 203, 928
HapII CCGG 3 cut(s) 174, 882, 1034
Hin1II CATG 5 cut(s) 71, 254, 316, 779, 960
HincII GTYRAC 2 cut(s) 115, 370
HindII GTYRAC 2 cut(s) 115, 370
HinfI GANTC 5 cut(s) 64, 116, 212, 377, 813
HpaII CCGG 3 cut(s) 174, 882, 1034
HphI GGTGA 2 cut(s) 698, 782
Hpy166II GTNNAC 5 cut(s) 115, 268, 283, 370, 781
Hpy188I TCNGA 2 cut(s) 364, 620
Hpy188III TCNNGA 6 cut(s) 127, 313, 433, 810, 980, 997
Hpy8I GTNNAC 5 cut(s) 115, 268, 283, 370, 781
HpyAV CCTTC 4 cut(s) 40, 322, 327, 1010
HpyCH4III ACNGT 3 cut(s) 83, 524, 647
HpyCH4IV ACGT 1 cut(s) 622
HpyCH4V TGCA 9 cut(s) 224, 283, 383, 500, 512, 719, 729, 824, 921
HpyF10VI GCNNNNNNNGC 2 cut(s) 506, 800
HpyF3I CTNAG 1 cut(s) 363
HpySE526I ACGT 1 cut(s) 622
Hsp92II CATG 5 cut(s) 71, 254, 316, 779, 960
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
Ksp22I TGATCA 1 cut(s) 772
Kzo9I GATC 6 cut(s) 423, 651, 772, 946, 976, 1002
LmnI GCTCC 4 cut(s) 406, 448, 873, 932
Lsp1109I GCAGC 2 cut(s) 496, 1017
MaeI CTAG 1 cut(s) 840
MaeII ACGT 1 cut(s) 622
MaeIII GTNAC 2 cut(s) 12, 694
MalI GATC 6 cut(s) 425, 653, 774, 948, 978, 1004
MboI GATC 6 cut(s) 423, 651, 772, 946, 976, 1002
MboII GAAGA 3 cut(s) 37, 544, 607
MfeI CAATTG 1 cut(s) 868
MflI RGATCY 1 cut(s) 651
MhlI GDGCHC 1 cut(s) 285
MlsI TGGCCA 1 cut(s) 183
MluCI AATT 2 cut(s) 185, 868
MluNI TGGCCA 1 cut(s) 183
MlyI GAGTC 4 cut(s) 58, 110, 386, 822
MmeI TCCRAC 1 cut(s) 470
Mox20I TGGCCA 1 cut(s) 183
MreI CGCCGGCG 1 cut(s) 173
MroNI GCCGGC 1 cut(s) 173
MscI TGGCCA 1 cut(s) 183
MseI TTAA 1 cut(s) 1045
MslI CAYNNNNRTG 2 cut(s) 576, 645
Msp20I TGGCCA 1 cut(s) 183
MspI CCGG 3 cut(s) 174, 882, 1034
MspR9I CCNGG 2 cut(s) 200, 930
MunI CAATTG 1 cut(s) 868
MvaI CCWGG 2 cut(s) 200, 930
MvnI CGCG 1 cut(s) 951
MwoI GCNNNNNNNGC 2 cut(s) 506, 800
NaeI GCCGGC 1 cut(s) 175
NcoI CCATGG 1 cut(s) 956
NdeII GATC 6 cut(s) 423, 651, 772, 946, 976, 1002
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 5 cut(s) 71, 254, 316, 779, 960
NlaIV GGNNCC 5 cut(s) 353, 444, 802, 927, 934
NmeAIII GCCGAG 2 cut(s) 934, 961
PagI TCATGA 1 cut(s) 312
PcsI WCGNNNNNNNCGW 1 cut(s) 1011
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 1 cut(s) 212
PkrI GCNGC 4 cut(s) 205, 487, 511, 1032
Ple19I CGATCG 1 cut(s) 1005
PleI GAGTC 4 cut(s) 58, 110, 385, 821
PpsI GAGTC 4 cut(s) 58, 110, 385, 821
Psp6I CCWGG 2 cut(s) 198, 928
PspGI CCWGG 2 cut(s) 198, 928
PspN4I GGNNCC 5 cut(s) 353, 444, 802, 927, 934
PspPI GGNCC 4 cut(s) 351, 843, 864, 926
PstI CTGCAG 1 cut(s) 226
PsuI RGATCY 1 cut(s) 651
PvuI CGATCG 1 cut(s) 1005
RsaI GTAC 3 cut(s) 269, 902, 914
RsaNI GTAC 3 cut(s) 268, 901, 913
RseI CAYNNNNRTG 2 cut(s) 576, 645
SalI GTCGAC 1 cut(s) 113
SaqAI TTAA 1 cut(s) 1045
SatI GCNGC 4 cut(s) 204, 486, 510, 1031
Sau3AI GATC 6 cut(s) 423, 651, 772, 946, 976, 1002
Sau96I GGNCC 4 cut(s) 351, 843, 864, 926
SbfI CCTGCAGG 1 cut(s) 226
SchI GAGTC 4 cut(s) 58, 110, 386, 822
ScrFI CCNGG 2 cut(s) 200, 930
SdaI CCTGCAGG 1 cut(s) 226
SduI GDGCHC 1 cut(s) 285
SfcI CTRYAG 2 cut(s) 222, 532
SgrAI CRCCGGYG 1 cut(s) 173
SinI GGWCC 3 cut(s) 351, 843, 864
SmiMI CAYNNNNRTG 2 cut(s) 576, 645
Sse8387I CCTGCAGG 1 cut(s) 226
Sse9I AATT 2 cut(s) 185, 868
SsiI CCGC 3 cut(s) 54, 204, 485
SspMI CTAG 1 cut(s) 840
StyD4I CCNGG 2 cut(s) 198, 928
StyI CCWWGG 3 cut(s) 595, 795, 956
TaaI ACNGT 3 cut(s) 83, 524, 647
TaiI ACGT 1 cut(s) 625
TaqI TCGA 8 cut(s) 114, 165, 210, 555, 606, 809, 1005, 1014
TasI AATT 2 cut(s) 185, 868
TatI WGTACW 2 cut(s) 267, 912
TauI GCSGC 2 cut(s) 206, 488
TfiI GAWTC 1 cut(s) 212
Tru1I TTAA 1 cut(s) 1045
Tru9I TTAA 1 cut(s) 1045
TscAI CASTG 4 cut(s) 242, 486, 527, 736
TseI GCWGC 2 cut(s) 509, 1030
TspDTI ATGAA 7 cut(s) 239, 301, 379, 400, 601, 733, 1000
TspGWI ACGGA 1 cut(s) 989
TspRI CASTG 4 cut(s) 242, 486, 527, 736
VneI GTGCAC 1 cut(s) 281
VpaK11BI GGWCC 3 cut(s) 351, 843, 864
XmiI GTMKAC 2 cut(s) 114, 780
XspI CTAG 1 cut(s) 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.