MD13G1153000.v1.1

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015931
Physical Location & Seq
Forward (+)
12001511 .. 12003077
1567 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1153000.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGGCTTGGTCTTCGTCCACAACATTCATAACCTTTGCAATCATTTTTTTCACCTACGTAACTCTCACGAATGTTGTTCATTGCTTGACAGCGCAAAAGGTCATTGATTCACCCTTATTGACTCAGAAGATTCACACGAACCGCACGCTTAAGGTTGACATCAATGGCAAGGGAGATTTCACATCTGTTCAAGCAGCCATTGATGCTGTTCCTGAAGGCAATAAGCAGTGGATCATCATCCATGTTAGGAAAGGAGTGTACAGAGAAAAGGTGATCATACCACGTAATAAGCCCTATATATTCATGAGAGGAAATGGGAAGGGGAGGTCAGCCATTGTTTGGTCCCAAAGCTCTTCGGACAATGTTGAATCTGCTACTTTTAGTGTCGAAGCTTCCCATTTCATTGCCTTCGGGATCAGCTTCAAGAATGAGGCCCCGACCGGTGTGGCTTACACCTCCCAGAACCAGTCAGTTGCAGCATTTGTAGCTGCAGACAAAGTTGCATTCTACCATTGTGGATTTTACAGTACCCATAACACCCTCTTCGATTACAAAGGCAGACATTACTATGATAATTGCTACATCCAGGGCTCCATTGACTTCATCTTTGGACGCGGTAGATCTGTCTTCCACAGCTGCGAGGTCTTTGTCATTGGAGACAAGAGGGTGTCGATCAAGGGGTCCGTGACAGCTCAAAATAGGGAGACTGAGAAGGAGAACAGTGGATTTGTTTTTAACAAAGGCAAGCTCTATGGTGTTGGTGACAGTGTGTACCTAGGTAGGGCAAAGGGCGCTTTCTCCAGAGTGATTTACGCAAACATGTACCTCTCCAAGACCGTCGTGCCACAAGGTTGGACCAACTGGAGCTATTCCGGCACAACTGAAAACCTATACCATGCCGAGTACAAGTGCAAAGGGCCAGGAGCAGAGGCTACAGGGCGTGCCGAATGGGCAAGACAACTCACCGAAAAAGAAGTTGCACCCTTCTTGTCTATCGACTTCATCAACGGCAAGGAATGGCTGCCAGTTTGGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

346

Amino Acids

38.63

Weight (kDa)

9.23

Isoelectric Point (pI)

27.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 52 - 337 2e-63 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015931)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17060
fragaria_vesca FvH4_4g19840
malus_domestica MD13G1153000.v1.1 MD16G1153400.v1.1
prunus_persica Prupe.1G116600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0425411
rosa_laevigata RLG00000007371
rosa_multiflora Rmu_sc0001900.1_g000046
rosa_roxburghii Rroxscaffold_5G00367370
rosa_rugosa Rorug04G0202700
rosa_samantha Rh4AG259900 Rh4BG265800 Rh4CG277100 Rh4DG261800
rosa_wichuraiana Rw4G022500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 339
AccII CGCG 1 cut(s) 615
AciI CCGC 2 cut(s) 142, 615
AclWI GGATC 2 cut(s) 239, 422
AcuI CTGAAG 1 cut(s) 234
AfaI GTAC 5 cut(s) 260, 529, 773, 824, 905
AfiI CCNNNNNNNGG 2 cut(s) 339, 781
AflII CTTAAG 1 cut(s) 149
AflIII ACRYGT 1 cut(s) 819
AgeI ACCGGT 1 cut(s) 440
AgsI TTSAA 3 cut(s) 191, 368, 424
AjnI CCWGG 2 cut(s) 585, 919
AluBI AGCT 8 cut(s) 351, 392, 420, 488, 636, 692, 748, 867
AluI AGCT 8 cut(s) 351, 392, 420, 488, 636, 692, 748, 867
Alw26I GTCTC 2 cut(s) 651, 698
AlwI GGATC 2 cut(s) 239, 422
AoxI GGCC 2 cut(s) 432, 917
ApeKI GCWGC 5 cut(s) 194, 476, 488, 636, 1021
ArsI GACNNNNNNTTYG 2 cut(s) 590, 622
AsiGI ACCGGT 1 cut(s) 440
AspA2I CCTAGG 1 cut(s) 775
AspLEI GCGC 2 cut(s) 94, 794
AspS9I GGNCC 5 cut(s) 342, 433, 681, 855, 917
AsuHPI GGTGA 5 cut(s) 43, 102, 283, 773, 955
AvaII GGWCC 3 cut(s) 342, 681, 855
AvrII CCTAGG 1 cut(s) 775
BanII GRGCYC 1 cut(s) 593
BbsI GAAGAC 2 cut(s) 3, 619
BbvI GCAGC 5 cut(s) 206, 475, 488, 623, 1008
BceAI ACGGC 1 cut(s) 1024
BciT130I CCWGG 2 cut(s) 587, 921
BclI TGATCA 1 cut(s) 273
BcoDI GTCTC 2 cut(s) 651, 698
BfaI CTAG 1 cut(s) 776
BfmI CTRYAG 2 cut(s) 489, 933
BfoI RGCGCY 1 cut(s) 795
BfrI CTTAAG 1 cut(s) 149
BglI GCCNNNNNGGC 2 cut(s) 950, 1030
BglII AGATCT 1 cut(s) 620
BisI GCNGC 5 cut(s) 195, 477, 489, 637, 1022
BlnI CCTAGG 1 cut(s) 775
BlsI GCNGC 5 cut(s) 196, 478, 490, 638, 1023
Bme1390I CCNGG 2 cut(s) 587, 921
Bme18I GGWCC 3 cut(s) 342, 681, 855
BmgT120I GGNCC 5 cut(s) 342, 433, 681, 855, 917
BmiI GGNNCC 4 cut(s) 344, 435, 592, 682
BmrFI CCNGG 2 cut(s) 587, 921
BmsI GCATC 1 cut(s) 193
BpiI GAAGAC 2 cut(s) 3, 619
BpmI CTGGAG 2 cut(s) 784, 883
BsaAI YACGTR 2 cut(s) 58, 284
BsaBI GATNNNNATC 1 cut(s) 236
BsaJI CCNNGG 2 cut(s) 586, 775
BsaWI WCCGGW 1 cut(s) 440
Bsc4I CCNNNNNNNGG 2 cut(s) 339, 781
Bse118I RCCGGY 1 cut(s) 440
Bse1I ACTGG 3 cut(s) 466, 866, 1025
Bse3DI GCAATG 2 cut(s) 79, 402
Bse8I GATNNNNATC 1 cut(s) 236
BseBI CCWGG 2 cut(s) 587, 921
BseDI CCNNGG 2 cut(s) 586, 775
BseGI GGATG 2 cut(s) 237, 582
BseJI GATNNNNATC 1 cut(s) 236
BseLI CCNNNNNNNGG 2 cut(s) 339, 781
BseMI GCAATG 2 cut(s) 79, 402
BseMII CTCAG 2 cut(s) 137, 699
BseNI ACTGG 3 cut(s) 466, 866, 1025
BseXI GCAGC 5 cut(s) 206, 475, 488, 623, 1008
Bsh1236I CGCG 1 cut(s) 615
Bsh1285I CGRYCG 1 cut(s) 441
BshFI GGCC 2 cut(s) 434, 919
BshTI ACCGGT 1 cut(s) 440
BsiEI CGRYCG 1 cut(s) 441
BsiSI CCGG 2 cut(s) 441, 873
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 2 cut(s) 339, 781
BsmAI GTCTC 2 cut(s) 651, 698
BsmFI GGGAC 1 cut(s) 328
BsmI GAATGC 1 cut(s) 503
BsnI GGCC 2 cut(s) 434, 919
Bsp1286I GDGCHC 1 cut(s) 593
Bsp1407I TGTACA 1 cut(s) 258
Bsp143I GATC 5 cut(s) 231, 273, 414, 620, 672
BspACI CCGC 2 cut(s) 142, 615
BspANI GGCC 2 cut(s) 434, 919
BspCNI CTCAG 2 cut(s) 136, 700
BspFNI CGCG 1 cut(s) 615
BspHI TCATGA 1 cut(s) 303
BspLI GGNNCC 4 cut(s) 344, 435, 592, 682
BspMAI CTGCAG 1 cut(s) 493
BspPI GGATC 2 cut(s) 239, 422
BspQI GCTCTTC 1 cut(s) 358
BspTI CTTAAG 1 cut(s) 149
BsrDI GCAATG 2 cut(s) 79, 402
BsrFI RCCGGY 1 cut(s) 440
BsrGI TGTACA 1 cut(s) 258
BsrI ACTGG 3 cut(s) 466, 866, 1025
BssAI RCCGGY 1 cut(s) 440
BssECI CCNNGG 2 cut(s) 586, 775
BssMI GATC 5 cut(s) 231, 273, 414, 620, 672
BssT1I CCWWGG 1 cut(s) 775
Bst2UI CCWGG 2 cut(s) 587, 921
Bst4CI ACNGT 4 cut(s) 527, 722, 767, 838
Bst6I CTCTTC 2 cut(s) 358, 548
BstAFI CTTAAG 1 cut(s) 149
BstAUI TGTACA 1 cut(s) 258
BstBAI YACGTR 2 cut(s) 58, 284
BstC8I GCNNGC 3 cut(s) 146, 746, 942
BstDEI CTNAG 2 cut(s) 123, 708
BstF5I GGATG 2 cut(s) 237, 582
BstFNI CGCG 1 cut(s) 615
BstH2I RGCGCY 1 cut(s) 795
BstHHI GCGC 2 cut(s) 94, 794
BstKTI GATC 5 cut(s) 234, 276, 417, 623, 675
BstMAI GTCTC 2 cut(s) 651, 698
BstMBI GATC 5 cut(s) 231, 273, 414, 620, 672
BstMCI CGRYCG 1 cut(s) 441
BstMWI GCNNNNNNNGC 6 cut(s) 203, 485, 791, 873, 950, 1030
BstNI CCWGG 2 cut(s) 587, 921
BstNSI RCATGY 1 cut(s) 823
BstSCI CCNGG 2 cut(s) 585, 919
BstSFI CTRYAG 2 cut(s) 489, 933
BstSNI TACGTA 1 cut(s) 58
BstUI CGCG 1 cut(s) 615
BstV1I GCAGC 5 cut(s) 206, 475, 488, 623, 1008
BstV2I GAAGAC 2 cut(s) 3, 619
BstX2I RGATCY 1 cut(s) 620
BstXI CCANNNNNNTGG 1 cut(s) 852
BstYI RGATCY 1 cut(s) 620
BsuRI GGCC 2 cut(s) 434, 919
BtsCI GGATG 2 cut(s) 237, 582
BtsI GCAGTG 1 cut(s) 233
BtsIMutI CAGTG 3 cut(s) 233, 727, 772
Cac8I GCNNGC 3 cut(s) 146, 746, 942
CciI TCATGA 1 cut(s) 303
CfoI GCGC 2 cut(s) 94, 794
Cfr10I RCCGGY 1 cut(s) 440
Cfr13I GGNCC 5 cut(s) 342, 433, 681, 855, 917
CseI GACGC 1 cut(s) 621
Csp6I GTAC 5 cut(s) 259, 528, 772, 823, 904
CspAI ACCGGT 1 cut(s) 440
CviAII CATG 4 cut(s) 242, 304, 820, 896
CviQI GTAC 5 cut(s) 259, 528, 772, 823, 904
DdeI CTNAG 2 cut(s) 123, 708
DpnI GATC 5 cut(s) 233, 275, 416, 622, 674
DpnII GATC 5 cut(s) 231, 273, 414, 620, 672
Eam1104I CTCTTC 2 cut(s) 358, 548
EarI CTCTTC 2 cut(s) 358, 548
Eco105I TACGTA 1 cut(s) 58
Eco130I CCWWGG 1 cut(s) 775
Eco24I GRGCYC 1 cut(s) 593
Eco47I GGWCC 3 cut(s) 342, 681, 855
Eco57I CTGAAG 1 cut(s) 234
EcoO109I RGGNCCY 1 cut(s) 433
EcoRII CCWGG 2 cut(s) 585, 919
EcoT14I CCWWGG 1 cut(s) 775
EcoT38I GRGCYC 1 cut(s) 593
ErhI CCWWGG 1 cut(s) 775
FaeI CATG 4 cut(s) 245, 307, 823, 899
FaqI GGGAC 1 cut(s) 328
FatI CATG 4 cut(s) 241, 303, 819, 895
FbaI TGATCA 1 cut(s) 273
Fnu4HI GCNGC 5 cut(s) 195, 477, 489, 637, 1022
FokI GGATG 2 cut(s) 224, 569
FriOI GRGCYC 1 cut(s) 593
Fsp4HI GCNGC 5 cut(s) 195, 477, 489, 637, 1022
FspBI CTAG 1 cut(s) 776
GlaI GCGC 2 cut(s) 93, 793
GluI GCNGC 5 cut(s) 195, 477, 489, 637, 1022
GsuI CTGGAG 2 cut(s) 784, 883
HaeII RGCGCY 1 cut(s) 795
HaeIII GGCC 2 cut(s) 434, 919
HapII CCGG 2 cut(s) 441, 873
HgaI GACGC 1 cut(s) 621
HhaI GCGC 2 cut(s) 94, 794
Hin1II CATG 4 cut(s) 245, 307, 823, 899
Hin6I GCGC 2 cut(s) 92, 792
HinP1I GCGC 2 cut(s) 92, 792
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HindIII AAGCTT 1 cut(s) 390
HinfI GANTC 4 cut(s) 107, 121, 130, 368
HpaII CCGG 2 cut(s) 441, 873
HphI GGTGA 5 cut(s) 43, 102, 283, 773, 955
Hpy166II GTNNAC 4 cut(s) 18, 157, 259, 772
Hpy188I TCNGA 2 cut(s) 126, 358
Hpy188III TCNNGA 6 cut(s) 67, 212, 304, 412, 424, 801
Hpy8I GTNNAC 4 cut(s) 18, 157, 259, 772
Hpy99I CGWCG 1 cut(s) 842
HpyAV CCTTC 5 cut(s) 209, 313, 418, 706, 994
HpyCH4III ACNGT 4 cut(s) 527, 722, 767, 838
HpyCH4IV ACGT 2 cut(s) 57, 283
HpyCH4V TGCA 6 cut(s) 38, 476, 491, 503, 912, 980
HpyF10VI GCNNNNNNNGC 6 cut(s) 203, 485, 791, 873, 950, 1030
HpyF3I CTNAG 2 cut(s) 123, 708
HpySE526I ACGT 2 cut(s) 57, 283
Hsp92II CATG 4 cut(s) 245, 307, 823, 899
HspAI GCGC 2 cut(s) 92, 792
Ksp22I TGATCA 1 cut(s) 273
Kzo9I GATC 5 cut(s) 231, 273, 414, 620, 672
LguI GCTCTTC 1 cut(s) 358
LmnI GCTCC 3 cut(s) 596, 864, 923
Lsp1109I GCAGC 5 cut(s) 206, 475, 488, 623, 1008
LweI GCATC 1 cut(s) 193
MaeI CTAG 1 cut(s) 776
MaeII ACGT 2 cut(s) 57, 283
MaeIII GTNAC 3 cut(s) 58, 685, 761
MalI GATC 5 cut(s) 233, 275, 416, 622, 674
MboI GATC 5 cut(s) 231, 273, 414, 620, 672
MboII GAAGA 5 cut(s) 3, 139, 345, 535, 619
MflI RGATCY 1 cut(s) 620
MhlI GDGCHC 1 cut(s) 593
MluCI AATT 1 cut(s) 574
MlyI GAGTC 1 cut(s) 115
MmeI TCCRAC 1 cut(s) 833
MnlI CCTC 9 cut(s) 302, 318, 424, 466, 551, 634, 657, 836, 922
MseI TTAA 2 cut(s) 150, 735
MslI CAYNNNNRTG 1 cut(s) 567
MspA1I CMGCKG 1 cut(s) 636
MspCI CTTAAG 1 cut(s) 149
MspI CCGG 2 cut(s) 441, 873
MspR9I CCNGG 2 cut(s) 587, 921
Mva1269I GAATGC 1 cut(s) 503
MvaI CCWGG 2 cut(s) 587, 921
MvnI CGCG 1 cut(s) 615
MwoI GCNNNNNNNGC 6 cut(s) 203, 485, 791, 873, 950, 1030
NdeII GATC 5 cut(s) 231, 273, 414, 620, 672
NlaIII CATG 4 cut(s) 245, 307, 823, 899
NlaIV GGNNCC 4 cut(s) 344, 435, 592, 682
NmeAIII GCCGAG 1 cut(s) 925
NmuCI GTSAC 2 cut(s) 685, 761
NspI RCATGY 1 cut(s) 823
PagI TCATGA 1 cut(s) 303
PciI ACATGT 1 cut(s) 819
PciSI GCTCTTC 1 cut(s) 358
PctI GAATGC 1 cut(s) 503
PfeI GAWTC 3 cut(s) 107, 130, 368
PflMI CCANNNNNTGG 1 cut(s) 339
PinAI ACCGGT 1 cut(s) 440
PkrI GCNGC 5 cut(s) 196, 478, 490, 638, 1023
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
Ppu21I YACGTR 2 cut(s) 58, 284
PscI ACATGT 1 cut(s) 819
Psp6I CCWGG 2 cut(s) 585, 919
PspGI CCWGG 2 cut(s) 585, 919
PspN4I GGNNCC 4 cut(s) 344, 435, 592, 682
PspPI GGNCC 5 cut(s) 342, 433, 681, 855, 917
PstI CTGCAG 1 cut(s) 493
PsuI RGATCY 1 cut(s) 620
PvuII CAGCTG 1 cut(s) 636
RsaI GTAC 5 cut(s) 260, 529, 773, 824, 905
RsaNI GTAC 5 cut(s) 259, 528, 772, 823, 904
RseI CAYNNNNRTG 1 cut(s) 567
SapI GCTCTTC 1 cut(s) 358
SaqAI TTAA 2 cut(s) 150, 735
SatI GCNGC 5 cut(s) 195, 477, 489, 637, 1022
Sau3AI GATC 5 cut(s) 231, 273, 414, 620, 672
Sau96I GGNCC 5 cut(s) 342, 433, 681, 855, 917
SchI GAGTC 1 cut(s) 115
ScrFI CCNGG 2 cut(s) 587, 921
SduI GDGCHC 1 cut(s) 593
SfaNI GCATC 1 cut(s) 193
SfcI CTRYAG 2 cut(s) 489, 933
SinI GGWCC 3 cut(s) 342, 681, 855
SmiMI CAYNNNNRTG 1 cut(s) 567
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
SnaBI TACGTA 1 cut(s) 58
Sse9I AATT 1 cut(s) 574
SsiI CCGC 2 cut(s) 142, 615
SspMI CTAG 1 cut(s) 776
StyD4I CCNGG 2 cut(s) 585, 919
StyI CCWWGG 1 cut(s) 775
TaaI ACNGT 4 cut(s) 527, 722, 767, 838
TaiI ACGT 2 cut(s) 60, 286
TaqI TCGA 4 cut(s) 387, 546, 671, 996
TasI AATT 1 cut(s) 574
TatI WGTACW 2 cut(s) 258, 903
TfiI GAWTC 3 cut(s) 107, 130, 368
Tru1I TTAA 2 cut(s) 150, 735
Tru9I TTAA 2 cut(s) 150, 735
TscAI CASTG 3 cut(s) 233, 727, 772
TseFI GTSAC 2 cut(s) 685, 761
TseI GCWGC 5 cut(s) 194, 476, 488, 636, 1021
Tsp45I GTSAC 2 cut(s) 685, 761
TspDTI ATGAA 6 cut(s) 16, 68, 292, 391, 592, 991
TspGWI ACGGA 1 cut(s) 673
TspRI CASTG 3 cut(s) 233, 727, 772
Van91I CCANNNNNTGG 1 cut(s) 339
Vha464I CTTAAG 1 cut(s) 149
VpaK11BI GGWCC 3 cut(s) 342, 681, 855
XceI RCATGY 1 cut(s) 823
XmaJI CCTAGG 1 cut(s) 775
XspI CTAG 1 cut(s) 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.