Rorug04G0202700

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0015931
Physical Location & Seq
Forward (+)
35176589 .. 35178238
1650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0202700.1

Sequence Viewer

Length: 609 bp
ATGGCCTTCGTCGTTTCAGCCGTCAAGCAAGAAATCCCCGAACTCTCCCGTCGTTTCATCCTAATTCTCCGACTTATTCGACGGATGCATGATGGCCATGGAGACGGTATCGACTTTAGGAGAGTTCGCGCCGGAGTCGAGGTCAGTGATGAATTTTGTTCAAATTGCAGACTAAGAAATGGTGAATTTATAAGAAAAGTTGTCCTCTCAGTCCTCTCTCAACACTCTCAGTCCTCCCTCTCTCAATTCTCTCTCACAAACCCTAAAGCGGCCGATTTGGACCTCTCTCTCGCCGACAAAAGAACGCTGGGCAAAGCAATCAATGGCCGCCTTGCTTGTGTGGGTGGATTGTATTGCTTGAGAATGAAGAAGGCTGGATTGGTCGAAAAAAATATGGTGAGCTGGAACTTCGCCGGCACATGGAAGGAAGCTGTTCACAGGGCTTGTTTTCTCACTTTTGCTTCCATGTTTGATATACTCTCAACTTGGACAAGCGATCACTTGGGAGAAAATTCTCAAGTGGATTACCAGGCTATGGTGGCACAGCTTGAACACCAATTTCTACTCAGAAGACTGTCCTTAGGCTGGTGGTATTACTGTCAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

202

Amino Acids

23.02

Weight (kDa)

9.27

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015931)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17060
fragaria_vesca FvH4_4g19840
malus_domestica MD13G1153000.v1.1 MD16G1153400.v1.1
prunus_persica Prupe.1G116600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0425411
rosa_laevigata RLG00000007371
rosa_multiflora Rmu_sc0001900.1_g000046
rosa_roxburghii Rroxscaffold_5G00367370
rosa_rugosa Rorug04G0202700
rosa_samantha Rh4AG259900 Rh4BG265800 Rh4CG277100 Rh4DG261800
rosa_wichuraiana Rw4G022500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 191
AccB7I CCANNNNNTGG 1 cut(s) 535
AccII CGCG 1 cut(s) 129
AciI CCGC 2 cut(s) 269, 328
AcoI YGGCCR 3 cut(s) 94, 270, 325
AcsI RAATTY 3 cut(s) 152, 185, 511
AfiI CCNNNNNNNGG 4 cut(s) 268, 420, 535, 585
AgsI TTSAA 2 cut(s) 162, 551
AjnI CCWGG 1 cut(s) 528
AjuI GAANNNNNNNTTGG 2 cut(s) 362, 394
AluBI AGCT 3 cut(s) 402, 431, 547
AluI AGCT 3 cut(s) 402, 431, 547
Alw26I GTCTC 1 cut(s) 96
AoxI GGCC 4 cut(s) 3, 94, 270, 325
ApoI RAATTY 3 cut(s) 152, 185, 511
Asp700I GAANNNNTTC 1 cut(s) 432
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 1 cut(s) 280
AsuHPI GGTGA 2 cut(s) 194, 409
AvaII GGWCC 1 cut(s) 280
AxyI CCTNAGG 1 cut(s) 580
BalI TGGCCA 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 577
BccI CCATC 1 cut(s) 86
BceAI ACGGC 1 cut(s) 5
BciT130I CCWGG 1 cut(s) 530
BcoDI GTCTC 1 cut(s) 96
BisI GCNGC 2 cut(s) 270, 328
BlsI GCNGC 2 cut(s) 271, 329
Bme1390I CCNGG 1 cut(s) 530
Bme18I GGWCC 1 cut(s) 280
BmgT120I GGNCC 1 cut(s) 280
BmrFI CCNGG 1 cut(s) 530
BmsI GCATC 1 cut(s) 75
BpiI GAAGAC 1 cut(s) 577
BpuEI CTTGAG 2 cut(s) 379, 501
BsaJI CCNNGG 1 cut(s) 97
BsaXI ACNNNNNCTCC 2 cut(s) 126, 156
Bsc4I CCNNNNNNNGG 4 cut(s) 268, 420, 535, 585
Bse118I RCCGGY 1 cut(s) 413
Bse21I CCTNAGG 1 cut(s) 580
BseBI CCWGG 1 cut(s) 530
BseDI CCNNGG 1 cut(s) 97
BseGI GGATG 2 cut(s) 57, 90
BseLI CCNNNNNNNGG 4 cut(s) 268, 420, 535, 585
BseMII CTCAG 3 cut(s) 222, 242, 580
BseX3I CGGCCG 1 cut(s) 270
BseYI CCCAGC 1 cut(s) 307
Bsh1236I CGCG 1 cut(s) 129
Bsh1285I CGRYCG 1 cut(s) 273
BshFI GGCC 4 cut(s) 5, 96, 272, 327
BsiEI CGRYCG 1 cut(s) 273
BsiSI CCGG 2 cut(s) 132, 414
BslI CCNNNNNNNGG 4 cut(s) 268, 420, 535, 585
BsmAI GTCTC 1 cut(s) 96
BsmBI CGTCTC 1 cut(s) 96
BsnI GGCC 4 cut(s) 5, 96, 272, 327
Bsp143I GATC 1 cut(s) 496
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 2 cut(s) 269, 328
BspANI GGCC 4 cut(s) 5, 96, 272, 327
BspCNI CTCAG 3 cut(s) 221, 241, 579
BspFNI CGCG 1 cut(s) 129
BsrFI RCCGGY 1 cut(s) 413
BssAI RCCGGY 1 cut(s) 413
BssECI CCNNGG 1 cut(s) 97
BssMI GATC 1 cut(s) 496
BssT1I CCWWGG 1 cut(s) 97
Bst2UI CCWGG 1 cut(s) 530
Bst4CI ACNGT 3 cut(s) 107, 576, 599
BstC8I GCNNGC 1 cut(s) 415
BstDEI CTNAG 5 cut(s) 173, 208, 228, 566, 580
BstDSI CCRYGG 1 cut(s) 97
BstF5I GGATG 2 cut(s) 57, 90
BstFNI CGCG 1 cut(s) 129
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 1 cut(s) 499
BstMAI GTCTC 1 cut(s) 96
BstMBI GATC 1 cut(s) 496
BstMCI CGRYCG 1 cut(s) 273
BstMWI GCNNNNNNNGC 1 cut(s) 539
BstNI CCWGG 1 cut(s) 530
BstSCI CCNGG 1 cut(s) 528
BstUI CGCG 1 cut(s) 129
BstV2I GAAGAC 1 cut(s) 577
BstZI CGGCCG 1 cut(s) 270
Bsu36I CCTNAGG 1 cut(s) 580
BsuRI GGCC 4 cut(s) 5, 96, 272, 327
BtgI CCRYGG 1 cut(s) 97
BtsCI GGATG 2 cut(s) 57, 90
BtsIMutI CAGTG 1 cut(s) 151
Cac8I GCNNGC 1 cut(s) 415
CfoI GCGC 1 cut(s) 131
Cfr10I RCCGGY 1 cut(s) 413
Cfr13I GGNCC 1 cut(s) 280
CviAII CATG 4 cut(s) 89, 98, 420, 466
DdeI CTNAG 5 cut(s) 173, 208, 228, 566, 580
DpnI GATC 1 cut(s) 498
DpnII GATC 1 cut(s) 496
EaeI YGGCCR 3 cut(s) 94, 270, 325
EagI CGGCCG 1 cut(s) 270
EclXI CGGCCG 1 cut(s) 270
Eco130I CCWWGG 1 cut(s) 97
Eco47I GGWCC 1 cut(s) 280
Eco52I CGGCCG 1 cut(s) 270
Eco81I CCTNAGG 1 cut(s) 580
EcoRII CCWGG 1 cut(s) 528
EcoT14I CCWWGG 1 cut(s) 97
EcoT22I ATGCAT 1 cut(s) 90
ErhI CCWWGG 1 cut(s) 97
Esp3I CGTCTC 1 cut(s) 96
FaeI CATG 4 cut(s) 92, 101, 423, 469
FaiI YATR 8 cut(s) 90, 99, 191, 395, 421, 467, 476, 536
FatI CATG 4 cut(s) 88, 97, 419, 465
Fnu4HI GCNGC 2 cut(s) 270, 328
FokI GGATG 2 cut(s) 44, 97
Fsp4HI GCNGC 2 cut(s) 270, 328
GlaI GCGC 1 cut(s) 130
GluI GCNGC 2 cut(s) 270, 328
GsaI CCCAGC 1 cut(s) 311
HaeIII GGCC 4 cut(s) 5, 96, 272, 327
HapII CCGG 2 cut(s) 132, 414
HhaI GCGC 1 cut(s) 131
Hin1II CATG 4 cut(s) 92, 101, 423, 469
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HinfI GANTC 1 cut(s) 135
HpaII CCGG 2 cut(s) 132, 414
HphI GGTGA 2 cut(s) 194, 409
Hpy166II GTNNAC 1 cut(s) 436
Hpy188I TCNGA 2 cut(s) 71, 569
Hpy8I GTNNAC 1 cut(s) 436
Hpy99I CGWCG 3 cut(s) 14, 54, 84
HpyAV CCTTC 3 cut(s) 16, 364, 418
HpyCH4III ACNGT 3 cut(s) 107, 576, 599
HpyCH4V TGCA 2 cut(s) 88, 168
HpyF10VI GCNNNNNNNGC 1 cut(s) 539
HpyF3I CTNAG 5 cut(s) 173, 208, 228, 566, 580
Hsp92II CATG 4 cut(s) 92, 101, 423, 469
HspAI GCGC 1 cut(s) 129
KroI GCCGGC 1 cut(s) 413
KroNI GCCGGC 1 cut(s) 415
Kzo9I GATC 1 cut(s) 496
LpnPI CCDG 9 cut(s) 145, 293, 360, 388, 424, 427, 515, 542, 571
LweI GCATC 1 cut(s) 75
MalI GATC 1 cut(s) 498
MboI GATC 1 cut(s) 496
MboII GAAGA 2 cut(s) 379, 582
MlsI TGGCCA 1 cut(s) 96
MluCI AATT 7 cut(s) 63, 152, 163, 185, 245, 511, 557
MluNI TGGCCA 1 cut(s) 96
MlyI GAGTC 1 cut(s) 144
MmeI TCCRAC 1 cut(s) 94
MnlI CCTC 6 cut(s) 133, 215, 224, 244, 248, 293
Mox20I TGGCCA 1 cut(s) 96
Mph1103I ATGCAT 1 cut(s) 90
MroNI GCCGGC 1 cut(s) 413
MroXI GAANNNNTTC 1 cut(s) 432
MscI TGGCCA 1 cut(s) 96
Msp20I TGGCCA 1 cut(s) 96
MspI CCGG 2 cut(s) 132, 414
MspR9I CCNGG 1 cut(s) 530
MvaI CCWGG 1 cut(s) 530
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 1 cut(s) 539
NaeI GCCGGC 1 cut(s) 415
NcoI CCATGG 1 cut(s) 97
NdeII GATC 1 cut(s) 496
NgoMIV GCCGGC 1 cut(s) 413
NlaIII CATG 4 cut(s) 92, 101, 423, 469
NsiI ATGCAT 1 cut(s) 90
PcsI WCGNNNNNNNCGW 1 cut(s) 18
PdiI GCCGGC 1 cut(s) 415
PdmI GAANNNNTTC 1 cut(s) 432
PflMI CCANNNNNTGG 1 cut(s) 535
PkrI GCNGC 2 cut(s) 271, 329
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
PsiI TTATAA 1 cut(s) 191
Psp6I CCWGG 1 cut(s) 528
PspFI CCCAGC 1 cut(s) 307
PspGI CCWGG 1 cut(s) 528
PspPI GGNCC 1 cut(s) 280
SatI GCNGC 2 cut(s) 270, 328
Sau3AI GATC 1 cut(s) 496
Sau96I GGNCC 1 cut(s) 280
SchI GAGTC 1 cut(s) 144
ScrFI CCNGG 1 cut(s) 530
SetI ASST 5 cut(s) 144, 285, 404, 433, 549
SfaNI GCATC 1 cut(s) 75
SinI GGWCC 1 cut(s) 280
SmlI CTYRAG 2 cut(s) 358, 516
SmoI CTYRAG 2 cut(s) 358, 516
Sse9I AATT 7 cut(s) 63, 152, 163, 185, 245, 511, 557
SsiI CCGC 2 cut(s) 269, 328
StyD4I CCNGG 1 cut(s) 528
StyI CCWWGG 1 cut(s) 97
TaaI ACNGT 3 cut(s) 107, 576, 599
TaqI TCGA 4 cut(s) 79, 111, 138, 384
TasI AATT 7 cut(s) 63, 152, 163, 185, 245, 511, 557
TauI GCSGC 2 cut(s) 272, 330
TscAI CASTG 1 cut(s) 151
TspDTI ATGAA 3 cut(s) 46, 165, 380
TspGWI ACGGA 1 cut(s) 97
TspRI CASTG 1 cut(s) 151
Van91I CCANNNNNTGG 1 cut(s) 535
VpaK11BI GGWCC 1 cut(s) 280
XapI RAATTY 3 cut(s) 152, 185, 511
XmnI GAANNNNTTC 1 cut(s) 432
Zsp2I ATGCAT 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.