Rmu_sc0001900.1_g000046

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001900.1
OG0015931
Physical Location & Seq
Forward (+)
225822 .. 227499
1678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001900.1_g000046.1.cds

Sequence Viewer

Length: 1032 bp
atgttcaagttacaaggtttagttgccttcgcaatcacattgttcgtctccgcctctttcactcatggcggccaccccagcgtcgaagatgaaaagatcggctctcccctcctgactcaagagctacacaccaacaacaccatcctggtcgatatcaacggcggtggccaattcaagtccatccaggccgccatcgattctgttcctgcaggcaacaataagtgggttatcatccatgttaggagaggggtgtacagagaaaaagtgcacgtaccccaagacaaatcccatattttcttgagaggcaatgggaggggaaagacacacattgtttggtcccaaagctctcatgacaacattgaatctgcaactttcaaagtagaatctccatacttcattgcctttggaatcagcttcaagaatgaggctccaactggagtggcatacacgtcgcaaaatcagtcggtggcggcatttgtgggtgcagacagggttgcattttaccactgtgctttctacagtacccataacacccttttcgattacaagggcaggcattactatgataactgctacatccaaggctcaatcgacttcatcttcggacgtgctaggtctaccttccatagctgtgagatttttgtgatttcagacaagagggtttccatcaaagggtctgtaacagctaatcaccgagagaatgcgaacgagtgcagtgggtttgtatttatcaaaggcaaggtgtatggtgtaggtgatcatgtttacttgggtagagccaagggtcccttttccagagtcgtttttgcagaaacctacctctctaagaccattgtgccccagggttggaccaattggagttacaccggaggaacagagaacctgttccacgcggagtacaattgcaaagggccaggagccgactttgcagagcgcgctccatgggcgaagcaactgacggagaaagaggccgcaccattcatgtcgatcgacttcatcgacggacgggaatggctcccggtgtggatatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

343

Amino Acids

38.3

Weight (kDa)

8.47

Isoelectric Point (pI)

36.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015931)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17060
fragaria_vesca FvH4_4g19840
malus_domestica MD13G1153000.v1.1 MD16G1153400.v1.1
prunus_persica Prupe.1G116600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0425411
rosa_laevigata RLG00000007371
rosa_multiflora Rmu_sc0001900.1_g000046
rosa_roxburghii Rroxscaffold_5G00367370
rosa_rugosa Rorug04G0202700
rosa_samantha Rh4AG259900 Rh4BG265800 Rh4CG277100 Rh4DG261800
rosa_wichuraiana Rw4G022500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 617
AccII CGCG 2 cut(s) 893, 936
AciI CCGC 7 cut(s) 51, 69, 162, 189, 470, 893, 972
AcoI YGGCCR 2 cut(s) 70, 166
AfaI GTAC 4 cut(s) 254, 273, 523, 899
AfiI CCNNNNNNNGG 2 cut(s) 672, 846
AflIII ACRYGT 1 cut(s) 447
AgsI TTSAA 5 cut(s) 7, 175, 362, 376, 418
AjiI CACGTC 2 cut(s) 450, 608
AjnI CCWGG 4 cut(s) 144, 183, 840, 913
AluBI AGCT 5 cut(s) 124, 345, 414, 630, 686
AluI AGCT 5 cut(s) 124, 345, 414, 630, 686
Alw21I GWGCWC 1 cut(s) 270
Alw26I GTCTC 1 cut(s) 52
Alw44I GTGCAC 1 cut(s) 266
AoxI GGCC 5 cut(s) 70, 166, 186, 911, 969
ApaLI GTGCAC 1 cut(s) 266
ArsI GACNNNNNNTTYG 2 cut(s) 584, 616
Asp700I GAANNNNTTC 1 cut(s) 884
AspLEI GCGC 2 cut(s) 936, 938
AspS9I GGNCC 4 cut(s) 336, 785, 849, 911
AsuC2I CCSGG 1 cut(s) 1019
AsuHPI GGTGA 2 cut(s) 683, 767
AvaII GGWCC 3 cut(s) 336, 785, 849
BaeGI GKGCMC 2 cut(s) 270, 840
BalI TGGCCA 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 270
BccI CCATC 4 cut(s) 149, 188, 200, 674
BceAI ACGGC 1 cut(s) 175
BcgI CGANNNNNNTGC 2 cut(s) 432, 466
BciT130I CCWGG 4 cut(s) 146, 185, 842, 915
BclI TGATCA 1 cut(s) 757
BcnI CCSGG 1 cut(s) 1019
BcoDI GTCTC 1 cut(s) 52
BfaI CTAG 1 cut(s) 612
BfmI CTRYAG 2 cut(s) 207, 517
BisI GCNGC 4 cut(s) 70, 189, 471, 972
BlsI GCNGC 4 cut(s) 71, 190, 472, 973
Bme1390I CCNGG 5 cut(s) 146, 185, 842, 915, 1019
Bme18I GGWCC 3 cut(s) 336, 785, 849
BmgBI CACGTC 2 cut(s) 450, 608
BmgT120I GGNCC 4 cut(s) 336, 785, 849, 911
BmiI GGNNCC 6 cut(s) 338, 429, 786, 787, 919, 1016
BmrFI CCNGG 5 cut(s) 146, 185, 842, 915, 1019
BpmI CTGGAG 1 cut(s) 456
BpuEI CTTGAG 2 cut(s) 102, 319
BpuMI CCSGG 1 cut(s) 1019
Bsa29I ATCGAT 1 cut(s) 195
BsaAI YACGTR 1 cut(s) 271
BsaJI CCNNGG 5 cut(s) 580, 780, 840, 841, 941
BsaWI WCCGGW 1 cut(s) 866
Bsc4I CCNNNNNNNGG 2 cut(s) 672, 846
Bse1I ACTGG 1 cut(s) 439
Bse3DI GCAATG 2 cut(s) 313, 396
BseBI CCWGG 4 cut(s) 146, 185, 842, 915
BseCI ATCGAT 1 cut(s) 195
BseDI CCNNGG 5 cut(s) 580, 780, 840, 841, 941
BseGI GGATG 4 cut(s) 141, 180, 231, 576
BseLI CCNNNNNNNGG 2 cut(s) 672, 846
BseMI GCAATG 2 cut(s) 313, 396
BseNI ACTGG 1 cut(s) 439
BsePI GCGCGC 1 cut(s) 934
BseSI GKGCMC 2 cut(s) 270, 840
BseYI CCCAGC 1 cut(s) 77
BsgI GTGCAG 2 cut(s) 504, 733
Bsh1236I CGCG 2 cut(s) 893, 936
Bsh1285I CGRYCG 1 cut(s) 990
BshFI GGCC 5 cut(s) 72, 168, 188, 913, 971
BshVI ATCGAT 1 cut(s) 195
BsiEI CGRYCG 1 cut(s) 990
BsiHKAI GWGCWC 1 cut(s) 270
BsiSI CCGG 2 cut(s) 867, 1019
BslFI GGGAC 2 cut(s) 322, 771
BslI CCNNNNNNNGG 2 cut(s) 672, 846
BsmAI GTCTC 1 cut(s) 52
BsmBI CGTCTC 1 cut(s) 52
BsmFI GGGAC 2 cut(s) 322, 771
BsmI GAATGC 1 cut(s) 706
BsnI GGCC 5 cut(s) 72, 168, 188, 913, 971
Bsp1286I GDGCHC 2 cut(s) 270, 840
Bsp1407I TGTACA 1 cut(s) 252
Bsp143I GATC 3 cut(s) 96, 757, 987
Bsp19I CCATGG 1 cut(s) 941
BspACI CCGC 7 cut(s) 51, 69, 162, 189, 470, 893, 972
BspANI GGCC 5 cut(s) 72, 168, 188, 913, 971
BspDI ATCGAT 1 cut(s) 195
BspFNI CGCG 2 cut(s) 893, 936
BspHI TCATGA 1 cut(s) 349
BspLI GGNNCC 6 cut(s) 338, 429, 786, 787, 919, 1016
BspMAI CTGCAG 1 cut(s) 211
BsrDI GCAATG 2 cut(s) 313, 396
BsrGI TGTACA 1 cut(s) 252
BsrI ACTGG 1 cut(s) 439
BssECI CCNNGG 5 cut(s) 580, 780, 840, 841, 941
BssHII GCGCGC 1 cut(s) 934
BssMI GATC 3 cut(s) 96, 757, 987
BssT1I CCWWGG 3 cut(s) 580, 780, 941
Bst2UI CCWGG 4 cut(s) 146, 185, 842, 915
Bst4CI ACNGT 2 cut(s) 509, 521
BstAUI TGTACA 1 cut(s) 252
BstBAI YACGTR 1 cut(s) 271
BstC8I GCNNGC 3 cut(s) 211, 554, 936
BstDEI CTNAG 1 cut(s) 825
BstDSI CCRYGG 1 cut(s) 941
BstF5I GGATG 4 cut(s) 141, 180, 231, 576
BstFNI CGCG 2 cut(s) 893, 936
BstHHI GCGC 2 cut(s) 936, 938
BstKTI GATC 3 cut(s) 99, 760, 990
BstMAI GTCTC 1 cut(s) 52
BstMBI GATC 3 cut(s) 96, 757, 987
BstMCI CGRYCG 1 cut(s) 990
BstMWI GCNNNNNNNGC 4 cut(s) 78, 926, 935, 944
BstNI CCWGG 4 cut(s) 146, 185, 842, 915
BstSCI CCNGG 5 cut(s) 144, 183, 840, 913, 1017
BstSFI CTRYAG 2 cut(s) 207, 517
BstSLI GKGCMC 2 cut(s) 270, 840
BstUI CGCG 2 cut(s) 893, 936
Bsu15I ATCGAT 1 cut(s) 195
BsuRI GGCC 5 cut(s) 72, 168, 188, 913, 971
BsuTUI ATCGAT 1 cut(s) 195
BtgI CCRYGG 1 cut(s) 941
BtrI CACGTC 2 cut(s) 450, 608
BtsCI GGATG 4 cut(s) 141, 180, 231, 576
BtsI GCAGTG 1 cut(s) 721
BtsIMutI CAGTG 2 cut(s) 505, 721
Cac8I GCNNGC 3 cut(s) 211, 554, 936
CciI TCATGA 1 cut(s) 349
CfoI GCGC 2 cut(s) 936, 938
Cfr13I GGNCC 4 cut(s) 336, 785, 849, 911
ClaI ATCGAT 1 cut(s) 195
CseI GACGC 1 cut(s) 70
Csp6I GTAC 4 cut(s) 253, 272, 522, 898
CspCI CAANNNNNGTGG 4 cut(s) 145, 180, 420, 455
CviAII CATG 6 cut(s) 65, 236, 350, 761, 942, 982
CviQI GTAC 4 cut(s) 253, 272, 522, 898
DdeI CTNAG 1 cut(s) 825
DpnI GATC 3 cut(s) 98, 759, 989
DpnII GATC 3 cut(s) 96, 757, 987
EaeI YGGCCR 2 cut(s) 70, 166
EciI GGCGGA 1 cut(s) 40
Eco130I CCWWGG 3 cut(s) 580, 780, 941
Eco32I GATATC 1 cut(s) 154
Eco47I GGWCC 3 cut(s) 336, 785, 849
EcoO109I RGGNCCY 1 cut(s) 785
EcoRII CCWGG 4 cut(s) 144, 183, 840, 913
EcoRV GATATC 1 cut(s) 154
EcoT14I CCWWGG 3 cut(s) 580, 780, 941
ErhI CCWWGG 3 cut(s) 580, 780, 941
Esp3I CGTCTC 1 cut(s) 52
FaeI CATG 6 cut(s) 68, 239, 353, 764, 945, 985
FalI AAGNNNNNCTT 2 cut(s) 773, 805
FaqI GGGAC 2 cut(s) 322, 771
FatI CATG 6 cut(s) 64, 235, 349, 760, 941, 981
FbaI TGATCA 1 cut(s) 757
FblI GTMKAC 1 cut(s) 617
Fnu4HI GCNGC 4 cut(s) 70, 189, 471, 972
FokI GGATG 4 cut(s) 128, 167, 218, 563
Fsp4HI GCNGC 4 cut(s) 70, 189, 471, 972
FspBI CTAG 1 cut(s) 612
GlaI GCGC 2 cut(s) 935, 937
GluI GCNGC 4 cut(s) 70, 189, 471, 972
GsaI CCCAGC 1 cut(s) 81
GsuI CTGGAG 1 cut(s) 456
HaeIII GGCC 5 cut(s) 72, 168, 188, 913, 971
HapII CCGG 2 cut(s) 867, 1019
HgaI GACGC 1 cut(s) 70
HhaI GCGC 2 cut(s) 936, 938
Hin1II CATG 6 cut(s) 68, 239, 353, 764, 945, 985
Hin6I GCGC 2 cut(s) 934, 936
HinP1I GCGC 2 cut(s) 934, 936
HinfI GANTC 6 cut(s) 115, 197, 362, 383, 408, 798
HpaII CCGG 2 cut(s) 867, 1019
HphI GGTGA 2 cut(s) 683, 767
Hpy166II GTNNAC 4 cut(s) 253, 268, 618, 766
Hpy188I TCNGA 2 cut(s) 605, 652
Hpy188III TCNNGA 6 cut(s) 112, 119, 298, 350, 418, 795
Hpy8I GTNNAC 4 cut(s) 253, 268, 618, 766
Hpy99I CGWCG 3 cut(s) 86, 454, 1004
HpyAV CCTTC 2 cut(s) 37, 631
HpyCH4III ACNGT 2 cut(s) 509, 521
HpyCH4IV ACGT 3 cut(s) 270, 449, 607
HpyCH4V TGCA 9 cut(s) 209, 268, 368, 485, 497, 714, 809, 906, 929
HpyF10VI GCNNNNNNNGC 4 cut(s) 78, 926, 935, 944
HpyF3I CTNAG 1 cut(s) 825
HpySE526I ACGT 3 cut(s) 270, 449, 607
Hsp92II CATG 6 cut(s) 68, 239, 353, 764, 945, 985
HspAI GCGC 2 cut(s) 934, 936
KflI GGGWCCC 1 cut(s) 785
Ksp22I TGATCA 1 cut(s) 757
Kzo9I GATC 3 cut(s) 96, 757, 987
LmnI GCTCC 4 cut(s) 433, 917, 943, 1020
MaeI CTAG 1 cut(s) 612
MaeII ACGT 3 cut(s) 270, 449, 607
MaeIII GTNAC 3 cut(s) 9, 679, 860
MalI GATC 3 cut(s) 98, 759, 989
MboI GATC 3 cut(s) 96, 757, 987
MboII GAAGA 2 cut(s) 98, 592
MfeI CAATTG 2 cut(s) 853, 901
MhlI GDGCHC 2 cut(s) 270, 840
MlsI TGGCCA 1 cut(s) 168
MluCI AATT 3 cut(s) 170, 853, 901
MluNI TGGCCA 1 cut(s) 168
MlyI GAGTC 2 cut(s) 109, 807
MmeI TCCRAC 2 cut(s) 455, 827
Mox20I TGGCCA 1 cut(s) 168
MroXI GAANNNNTTC 1 cut(s) 884
MscI TGGCCA 1 cut(s) 168
MslI CAYNNNNRTG 2 cut(s) 561, 630
Msp20I TGGCCA 1 cut(s) 168
MspI CCGG 2 cut(s) 867, 1019
MspR9I CCNGG 5 cut(s) 146, 185, 842, 915, 1019
MunI CAATTG 2 cut(s) 853, 901
Mva1269I GAATGC 1 cut(s) 706
MvaI CCWGG 4 cut(s) 146, 185, 842, 915
MvnI CGCG 2 cut(s) 893, 936
MwoI GCNNNNNNNGC 4 cut(s) 78, 926, 935, 944
NciI CCSGG 1 cut(s) 1019
NcoI CCATGG 1 cut(s) 941
NdeII GATC 3 cut(s) 96, 757, 987
NlaIII CATG 6 cut(s) 68, 239, 353, 764, 945, 985
NlaIV GGNNCC 6 cut(s) 338, 429, 786, 787, 919, 1016
PagI TCATGA 1 cut(s) 349
PasI CCCWGGG 1 cut(s) 841
PauI GCGCGC 1 cut(s) 934
PcsI WCGNNNNNNNCGW 1 cut(s) 996
PctI GAATGC 1 cut(s) 706
PdmI GAANNNNTTC 1 cut(s) 884
PfeI GAWTC 4 cut(s) 197, 362, 383, 408
PkrI GCNGC 4 cut(s) 71, 190, 472, 973
Ple19I CGATCG 1 cut(s) 990
PleI GAGTC 2 cut(s) 109, 806
PpsI GAGTC 2 cut(s) 109, 806
Ppu21I YACGTR 1 cut(s) 271
PpuMI RGGWCCY 1 cut(s) 785
Psp5II RGGWCCY 1 cut(s) 785
Psp6I CCWGG 4 cut(s) 144, 183, 840, 913
PspFI CCCAGC 1 cut(s) 77
PspGI CCWGG 4 cut(s) 144, 183, 840, 913
PspN4I GGNNCC 6 cut(s) 338, 429, 786, 787, 919, 1016
PspPI GGNCC 4 cut(s) 336, 785, 849, 911
PspPPI RGGWCCY 1 cut(s) 785
PstI CTGCAG 1 cut(s) 211
PteI GCGCGC 1 cut(s) 934
PvuI CGATCG 1 cut(s) 990
RsaI GTAC 4 cut(s) 254, 273, 523, 899
RsaNI GTAC 4 cut(s) 253, 272, 522, 898
RseI CAYNNNNRTG 2 cut(s) 561, 630
SatI GCNGC 4 cut(s) 70, 189, 471, 972
Sau3AI GATC 3 cut(s) 96, 757, 987
Sau96I GGNCC 4 cut(s) 336, 785, 849, 911
SbfI CCTGCAGG 1 cut(s) 211
SchI GAGTC 2 cut(s) 109, 807
ScrFI CCNGG 5 cut(s) 146, 185, 842, 915, 1019
SdaI CCTGCAGG 1 cut(s) 211
SduI GDGCHC 2 cut(s) 270, 840
SfcI CTRYAG 2 cut(s) 207, 517
SinI GGWCC 3 cut(s) 336, 785, 849
SmiMI CAYNNNNRTG 2 cut(s) 561, 630
SmlI CTYRAG 2 cut(s) 117, 298
SmoI CTYRAG 2 cut(s) 117, 298
Sse8387I CCTGCAGG 1 cut(s) 211
Sse9I AATT 3 cut(s) 170, 853, 901
SsiI CCGC 7 cut(s) 51, 69, 162, 189, 470, 893, 972
SspMI CTAG 1 cut(s) 612
StyD4I CCNGG 5 cut(s) 144, 183, 840, 913, 1017
StyI CCWWGG 3 cut(s) 580, 780, 941
TaaI ACNGT 2 cut(s) 509, 521
TaiI ACGT 3 cut(s) 273, 452, 610
TaqI TCGA 8 cut(s) 84, 150, 195, 540, 591, 986, 990, 999
TasI AATT 3 cut(s) 170, 853, 901
TatI WGTACW 2 cut(s) 252, 897
TauI GCSGC 4 cut(s) 72, 191, 473, 974
TfiI GAWTC 4 cut(s) 197, 362, 383, 408
TscAI CASTG 2 cut(s) 512, 721
TspDTI ATGAA 5 cut(s) 105, 385, 586, 970, 985
TspGWI ACGGA 2 cut(s) 974, 1017
TspRI CASTG 2 cut(s) 512, 721
VneI GTGCAC 1 cut(s) 266
VpaK11BI GGWCC 3 cut(s) 336, 785, 849
XmiI GTMKAC 1 cut(s) 617
XmnI GAANNNNTTC 1 cut(s) 884
XspI CTAG 1 cut(s) 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.