MD16G1153400.v1.1

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015931
Physical Location & Seq
Forward (+)
12178264 .. 12180099
1836 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1153400.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGGCTTGGTCTTCGTCCACAACATTCATCACCTTTGCAATCATTTTTTTCACCTCAGTAACTCTCACGAATGTTGTTCATGGCTTAACCGCACAAAAGGTAATAGATTCCCCCTTATTGACTCAGAAGATTCACACGAACCGCACGCTTAAGGTTGACATCAACGGCAAGGGAGATTTCACATCTGTTCAAGCAGCCATTGATGCTGTTCCTGAAGGCAATAAGCAATGGATCATCATCCATGTTAGGAAAGGAGTGTACAGAGAAAAGGTGATCATACCACGTAATAAGCCCTACATATTCATGAGAGGAAATGGGAAGGGCAGGTCCGCCATTGTTTGGTCACAAAGCTCTTCGGACAATGTCGAATCTGCTACTTTCAGTGTCGAAGCTCCCAATTTCATTGCCTTCGGGATCAGCTTCAAGAATGAGGCCCCGACCGGTGTGGCTTACACCTCGCAGAACCAGTCAGTTGCAGCATTTGTAGCTGCAGACAAAGTTGCATTCTACCATTGTGGATTTTACAGTACCCATAACACCCTCTTCGATTACAAGGGCAGACATTACTATGATAATTGTTACATCCAGGGCTCCATTGACTTCATCTTTGGCCGTGGTAGATCTGTCTTCCACAGCTGCGAGGTCTTTGTTATCGGAGACAAGAGGGTGTCGATCAAGGGGTCCGTGACAGCTCAAAATAGGGAGACTGAGAAGGAGAACAGTGGATTTGTTTTTAACAAAGGCAAGCTCTATGGTGTTGGTGACAGTGTGTACCTAGGTAGGGCAAAGGGTGCTTTCTCCAGAGTGATTTATGCAAACATGTACCTCTCGAAGACTGTCGTGCCACAAGGATGGACCAACTGGAGCTATTCCGGCGGAACTGAAAACTTATACCATGCCGAGTACAAGTGCAAAGGGCCAGGAGCTGAGGCTACAGGGCGTGCCGAATGGGCGAGACAACTCACCGAAAAAGAAGTTGCACCCTTCTTGTCTATCGACTTCATCAATGGCCAGGAATGGCTGCCAGTTTGGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

346

Amino Acids

38.45

Weight (kDa)

9.23

Isoelectric Point (pI)

27.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 52 - 337 2.8e-63 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015931)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17060
fragaria_vesca FvH4_4g19840
malus_domestica MD13G1153000.v1.1 MD16G1153400.v1.1
prunus_persica Prupe.1G116600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0425411
rosa_laevigata RLG00000007371
rosa_multiflora Rmu_sc0001900.1_g000046
rosa_roxburghii Rroxscaffold_5G00367370
rosa_rugosa Rorug04G0202700
rosa_samantha Rh4AG259900 Rh4BG265800 Rh4CG277100 Rh4DG261800
rosa_wichuraiana Rw4G022500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 315
AccB7I CCANNNNNTGG 1 cut(s) 339
AciI CCGC 4 cut(s) 90, 142, 330, 876
AclWI GGATC 2 cut(s) 239, 422
AcoI YGGCCR 2 cut(s) 610, 1009
AcuI CTGAAG 1 cut(s) 234
AfaI GTAC 5 cut(s) 260, 529, 773, 824, 905
AfiI CCNNNNNNNGG 2 cut(s) 339, 781
AflII CTTAAG 1 cut(s) 149
AflIII ACRYGT 1 cut(s) 819
AgeI ACCGGT 1 cut(s) 440
AgsI TTSAA 2 cut(s) 191, 424
AjnI CCWGG 3 cut(s) 585, 919, 1011
AluBI AGCT 9 cut(s) 351, 392, 420, 488, 636, 692, 748, 867, 926
AluI AGCT 9 cut(s) 351, 392, 420, 488, 636, 692, 748, 867, 926
Alw26I GTCTC 3 cut(s) 651, 698, 949
AlwI GGATC 2 cut(s) 239, 422
AlwNI CAGNNNCTG 1 cut(s) 926
AoxI GGCC 4 cut(s) 432, 610, 917, 1009
ApeKI GCWGC 5 cut(s) 194, 476, 488, 636, 1021
ArsI GACNNNNNNTTYG 2 cut(s) 590, 622
AsiGI ACCGGT 1 cut(s) 440
AspA2I CCTAGG 1 cut(s) 775
AspS9I GGNCC 5 cut(s) 327, 433, 681, 855, 917
AsuHPI GGTGA 5 cut(s) 22, 43, 283, 773, 955
AvaII GGWCC 3 cut(s) 327, 681, 855
AvrII CCTAGG 1 cut(s) 775
BalI TGGCCA 1 cut(s) 1011
BanII GRGCYC 1 cut(s) 593
BbsI GAAGAC 3 cut(s) 3, 619, 839
BbvCI CCTCAGC 1 cut(s) 927
BbvI GCAGC 5 cut(s) 206, 475, 488, 623, 1008
BccI CCATC 1 cut(s) 846
BceAI ACGGC 2 cut(s) 181, 597
BciT130I CCWGG 3 cut(s) 587, 921, 1013
BclI TGATCA 1 cut(s) 273
BcoDI GTCTC 3 cut(s) 651, 698, 949
BfaI CTAG 1 cut(s) 776
BfmI CTRYAG 2 cut(s) 489, 933
BfrI CTTAAG 1 cut(s) 149
BfuAI ACCTGC 1 cut(s) 315
BglI GCCNNNNNGGC 2 cut(s) 950, 1030
BglII AGATCT 1 cut(s) 620
BisI GCNGC 5 cut(s) 195, 477, 489, 637, 1022
BlnI CCTAGG 1 cut(s) 775
BlsI GCNGC 5 cut(s) 196, 478, 490, 638, 1023
Bme1390I CCNGG 3 cut(s) 587, 921, 1013
Bme18I GGWCC 3 cut(s) 327, 681, 855
BmgT120I GGNCC 5 cut(s) 327, 433, 681, 855, 917
BmiI GGNNCC 3 cut(s) 435, 592, 682
BmrFI CCNGG 3 cut(s) 587, 921, 1013
BmsI GCATC 1 cut(s) 193
BpiI GAAGAC 3 cut(s) 3, 619, 839
BpmI CTGGAG 2 cut(s) 784, 883
Bpu10I CCTNAGC 1 cut(s) 927
BsaAI YACGTR 1 cut(s) 284
BsaBI GATNNNNATC 1 cut(s) 236
BsaJI CCNNGG 3 cut(s) 586, 613, 775
BsaWI WCCGGW 1 cut(s) 440
Bsc4I CCNNNNNNNGG 2 cut(s) 339, 781
Bse118I RCCGGY 1 cut(s) 440
Bse1I ACTGG 3 cut(s) 466, 866, 1025
Bse3DI GCAATG 2 cut(s) 233, 402
Bse8I GATNNNNATC 1 cut(s) 236
BseBI CCWGG 3 cut(s) 587, 921, 1013
BseDI CCNNGG 3 cut(s) 586, 613, 775
BseGI GGATG 3 cut(s) 237, 582, 857
BseJI GATNNNNATC 1 cut(s) 236
BseLI CCNNNNNNNGG 2 cut(s) 339, 781
BseMI GCAATG 2 cut(s) 233, 402
BseMII CTCAG 4 cut(s) 69, 137, 699, 918
BseNI ACTGG 3 cut(s) 466, 866, 1025
BseXI GCAGC 5 cut(s) 206, 475, 488, 623, 1008
Bsh1285I CGRYCG 1 cut(s) 441
BshFI GGCC 4 cut(s) 434, 612, 919, 1011
BshTI ACCGGT 1 cut(s) 440
BsiEI CGRYCG 1 cut(s) 441
BsiSI CCGG 2 cut(s) 441, 873
BslI CCNNNNNNNGG 2 cut(s) 339, 781
BsmAI GTCTC 3 cut(s) 651, 698, 949
BsmI GAATGC 1 cut(s) 503
BsnI GGCC 4 cut(s) 434, 612, 919, 1011
Bsp1286I GDGCHC 1 cut(s) 593
Bsp1407I TGTACA 1 cut(s) 258
Bsp143I GATC 5 cut(s) 231, 273, 414, 620, 672
BspACI CCGC 4 cut(s) 90, 142, 330, 876
BspANI GGCC 4 cut(s) 434, 612, 919, 1011
BspCNI CTCAG 4 cut(s) 68, 136, 700, 919
BspHI TCATGA 1 cut(s) 303
BspLI GGNNCC 3 cut(s) 435, 592, 682
BspMAI CTGCAG 1 cut(s) 493
BspMI ACCTGC 1 cut(s) 315
BspPI GGATC 2 cut(s) 239, 422
BspQI GCTCTTC 1 cut(s) 358
BspTI CTTAAG 1 cut(s) 149
BsrDI GCAATG 2 cut(s) 233, 402
BsrFI RCCGGY 1 cut(s) 440
BsrGI TGTACA 1 cut(s) 258
BsrI ACTGG 3 cut(s) 466, 866, 1025
BssAI RCCGGY 1 cut(s) 440
BssECI CCNNGG 3 cut(s) 586, 613, 775
BssMI GATC 5 cut(s) 231, 273, 414, 620, 672
BssT1I CCWWGG 1 cut(s) 775
Bst2UI CCWGG 3 cut(s) 587, 921, 1013
Bst4CI ACNGT 4 cut(s) 527, 722, 767, 838
Bst6I CTCTTC 2 cut(s) 358, 548
BstAFI CTTAAG 1 cut(s) 149
BstAPI GCANNNNNTGC 1 cut(s) 791
BstAUI TGTACA 1 cut(s) 258
BstBAI YACGTR 1 cut(s) 284
BstC8I GCNNGC 3 cut(s) 146, 746, 942
BstDEI CTNAG 4 cut(s) 55, 123, 708, 927
BstDSI CCRYGG 1 cut(s) 613
BstF5I GGATG 3 cut(s) 237, 582, 857
BstKTI GATC 5 cut(s) 234, 276, 417, 623, 675
BstMAI GTCTC 3 cut(s) 651, 698, 949
BstMBI GATC 5 cut(s) 231, 273, 414, 620, 672
BstMCI CGRYCG 1 cut(s) 441
BstMWI GCNNNNNNNGC 6 cut(s) 203, 485, 791, 873, 950, 1030
BstNI CCWGG 3 cut(s) 587, 921, 1013
BstNSI RCATGY 1 cut(s) 823
BstSCI CCNGG 3 cut(s) 585, 919, 1011
BstSFI CTRYAG 2 cut(s) 489, 933
BstV1I GCAGC 5 cut(s) 206, 475, 488, 623, 1008
BstV2I GAAGAC 3 cut(s) 3, 619, 839
BstX2I RGATCY 1 cut(s) 620
BstXI CCANNNNNNTGG 1 cut(s) 852
BstYI RGATCY 1 cut(s) 620
BsuRI GGCC 4 cut(s) 434, 612, 919, 1011
BtgI CCRYGG 1 cut(s) 613
BtsCI GGATG 3 cut(s) 237, 582, 857
BtsIMutI CAGTG 3 cut(s) 388, 727, 772
BveI ACCTGC 1 cut(s) 315
Cac8I GCNNGC 3 cut(s) 146, 746, 942
CaiI CAGNNNCTG 1 cut(s) 926
CciI TCATGA 1 cut(s) 303
Cfr10I RCCGGY 1 cut(s) 440
Cfr13I GGNCC 5 cut(s) 327, 433, 681, 855, 917
Csp6I GTAC 5 cut(s) 259, 528, 772, 823, 904
CspAI ACCGGT 1 cut(s) 440
CviAII CATG 5 cut(s) 80, 242, 304, 820, 896
CviQI GTAC 5 cut(s) 259, 528, 772, 823, 904
DdeI CTNAG 4 cut(s) 55, 123, 708, 927
DpnI GATC 5 cut(s) 233, 275, 416, 622, 674
DpnII GATC 5 cut(s) 231, 273, 414, 620, 672
EaeI YGGCCR 2 cut(s) 610, 1009
Eam1104I CTCTTC 2 cut(s) 358, 548
EarI CTCTTC 2 cut(s) 358, 548
EciI GGCGGA 2 cut(s) 319, 891
Eco130I CCWWGG 1 cut(s) 775
Eco24I GRGCYC 1 cut(s) 593
Eco47I GGWCC 3 cut(s) 327, 681, 855
Eco57I CTGAAG 1 cut(s) 234
EcoO109I RGGNCCY 1 cut(s) 433
EcoRII CCWGG 3 cut(s) 585, 919, 1011
EcoT14I CCWWGG 1 cut(s) 775
EcoT38I GRGCYC 1 cut(s) 593
ErhI CCWWGG 1 cut(s) 775
FaeI CATG 5 cut(s) 83, 245, 307, 823, 899
FatI CATG 5 cut(s) 79, 241, 303, 819, 895
FbaI TGATCA 1 cut(s) 273
Fnu4HI GCNGC 5 cut(s) 195, 477, 489, 637, 1022
FokI GGATG 3 cut(s) 224, 569, 864
FriOI GRGCYC 1 cut(s) 593
Fsp4HI GCNGC 5 cut(s) 195, 477, 489, 637, 1022
FspBI CTAG 1 cut(s) 776
GluI GCNGC 5 cut(s) 195, 477, 489, 637, 1022
GsuI CTGGAG 2 cut(s) 784, 883
HaeIII GGCC 4 cut(s) 434, 612, 919, 1011
HapII CCGG 2 cut(s) 441, 873
Hin1II CATG 5 cut(s) 83, 245, 307, 823, 899
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HinfI GANTC 4 cut(s) 107, 121, 130, 368
HpaII CCGG 2 cut(s) 441, 873
HphI GGTGA 5 cut(s) 22, 43, 283, 773, 955
Hpy166II GTNNAC 4 cut(s) 18, 157, 259, 772
Hpy188I TCNGA 3 cut(s) 126, 358, 656
Hpy188III TCNNGA 7 cut(s) 67, 212, 304, 412, 424, 801, 829
Hpy8I GTNNAC 4 cut(s) 18, 157, 259, 772
HpyAV CCTTC 5 cut(s) 209, 313, 418, 706, 994
HpyCH4III ACNGT 4 cut(s) 527, 722, 767, 838
HpyCH4IV ACGT 1 cut(s) 283
HpyCH4V TGCA 7 cut(s) 38, 476, 491, 503, 815, 912, 980
HpyF10VI GCNNNNNNNGC 6 cut(s) 203, 485, 791, 873, 950, 1030
HpyF3I CTNAG 4 cut(s) 55, 123, 708, 927
HpySE526I ACGT 1 cut(s) 283
Hsp92II CATG 5 cut(s) 83, 245, 307, 823, 899
Ksp22I TGATCA 1 cut(s) 273
Kzo9I GATC 5 cut(s) 231, 273, 414, 620, 672
LguI GCTCTTC 1 cut(s) 358
LmnI GCTCC 4 cut(s) 397, 596, 864, 923
Lsp1109I GCAGC 5 cut(s) 206, 475, 488, 623, 1008
LweI GCATC 1 cut(s) 193
MaeI CTAG 1 cut(s) 776
MaeII ACGT 1 cut(s) 283
MaeIII GTNAC 5 cut(s) 58, 342, 578, 685, 761
MalI GATC 5 cut(s) 233, 275, 416, 622, 674
MboI GATC 5 cut(s) 231, 273, 414, 620, 672
MboII GAAGA 6 cut(s) 3, 139, 345, 535, 619, 844
MflI RGATCY 1 cut(s) 620
MhlI GDGCHC 1 cut(s) 593
MlsI TGGCCA 1 cut(s) 1011
MluCI AATT 2 cut(s) 397, 574
MluNI TGGCCA 1 cut(s) 1011
MlyI GAGTC 1 cut(s) 115
MnlI CCTC 9 cut(s) 64, 302, 424, 466, 551, 634, 657, 836, 922
Mox20I TGGCCA 1 cut(s) 1011
MscI TGGCCA 1 cut(s) 1011
MseI TTAA 3 cut(s) 86, 150, 735
MslI CAYNNNNRTG 3 cut(s) 302, 567, 850
Msp20I TGGCCA 1 cut(s) 1011
MspA1I CMGCKG 1 cut(s) 636
MspCI CTTAAG 1 cut(s) 149
MspI CCGG 2 cut(s) 441, 873
MspR9I CCNGG 3 cut(s) 587, 921, 1013
Mva1269I GAATGC 1 cut(s) 503
MvaI CCWGG 3 cut(s) 587, 921, 1013
MwoI GCNNNNNNNGC 6 cut(s) 203, 485, 791, 873, 950, 1030
NdeII GATC 5 cut(s) 231, 273, 414, 620, 672
NlaIII CATG 5 cut(s) 83, 245, 307, 823, 899
NlaIV GGNNCC 3 cut(s) 435, 592, 682
NmeAIII GCCGAG 1 cut(s) 925
NmuCI GTSAC 3 cut(s) 342, 685, 761
NspI RCATGY 1 cut(s) 823
PagI TCATGA 1 cut(s) 303
PciI ACATGT 1 cut(s) 819
PciSI GCTCTTC 1 cut(s) 358
PctI GAATGC 1 cut(s) 503
PfeI GAWTC 3 cut(s) 107, 130, 368
PflFI GACNNNGTC 1 cut(s) 362
PflMI CCANNNNNTGG 1 cut(s) 339
PinAI ACCGGT 1 cut(s) 440
PkrI GCNGC 5 cut(s) 196, 478, 490, 638, 1023
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
Ppu21I YACGTR 1 cut(s) 284
PscI ACATGT 1 cut(s) 819
Psp6I CCWGG 3 cut(s) 585, 919, 1011
PspGI CCWGG 3 cut(s) 585, 919, 1011
PspN4I GGNNCC 3 cut(s) 435, 592, 682
PspPI GGNCC 5 cut(s) 327, 433, 681, 855, 917
PstI CTGCAG 1 cut(s) 493
PstNI CAGNNNCTG 1 cut(s) 926
PsuI RGATCY 1 cut(s) 620
PsyI GACNNNGTC 1 cut(s) 362
PvuII CAGCTG 1 cut(s) 636
RsaI GTAC 5 cut(s) 260, 529, 773, 824, 905
RsaNI GTAC 5 cut(s) 259, 528, 772, 823, 904
RseI CAYNNNNRTG 3 cut(s) 302, 567, 850
SapI GCTCTTC 1 cut(s) 358
SaqAI TTAA 3 cut(s) 86, 150, 735
SatI GCNGC 5 cut(s) 195, 477, 489, 637, 1022
Sau3AI GATC 5 cut(s) 231, 273, 414, 620, 672
Sau96I GGNCC 5 cut(s) 327, 433, 681, 855, 917
SchI GAGTC 1 cut(s) 115
ScrFI CCNGG 3 cut(s) 587, 921, 1013
SduI GDGCHC 1 cut(s) 593
SfaNI GCATC 1 cut(s) 193
SfcI CTRYAG 2 cut(s) 489, 933
SinI GGWCC 3 cut(s) 327, 681, 855
SmiMI CAYNNNNRTG 3 cut(s) 302, 567, 850
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 2 cut(s) 397, 574
SsiI CCGC 4 cut(s) 90, 142, 330, 876
SspMI CTAG 1 cut(s) 776
StyD4I CCNGG 3 cut(s) 585, 919, 1011
StyI CCWWGG 1 cut(s) 775
TaaI ACNGT 4 cut(s) 527, 722, 767, 838
TaiI ACGT 1 cut(s) 286
TaqI TCGA 6 cut(s) 366, 387, 546, 671, 830, 996
TasI AATT 2 cut(s) 397, 574
TatI WGTACW 2 cut(s) 258, 903
TfiI GAWTC 3 cut(s) 107, 130, 368
Tru1I TTAA 3 cut(s) 86, 150, 735
Tru9I TTAA 3 cut(s) 86, 150, 735
TscAI CASTG 3 cut(s) 388, 727, 772
TseFI GTSAC 3 cut(s) 342, 685, 761
TseI GCWGC 5 cut(s) 194, 476, 488, 636, 1021
Tsp45I GTSAC 3 cut(s) 342, 685, 761
TspDTI ATGAA 6 cut(s) 16, 68, 292, 391, 592, 991
TspGWI ACGGA 1 cut(s) 673
TspRI CASTG 3 cut(s) 388, 727, 772
Tth111I GACNNNGTC 1 cut(s) 362
Van91I CCANNNNNTGG 1 cut(s) 339
Vha464I CTTAAG 1 cut(s) 149
VpaK11BI GGWCC 3 cut(s) 327, 681, 855
XceI RCATGY 1 cut(s) 823
XmaJI CCTAGG 1 cut(s) 775
XspI CTAG 1 cut(s) 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.