FvH4_4g20200

gal1,galk

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014620
Physical Location & Seq
Reverse (-)
23536046 .. 23541103
5058 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g20200.t1

Sequence Viewer

Length: 1500 bp
ATGGCTCAAATGATGAGGCACGAAGAACTTCCGATCCCAATTCACTCGGCGCTCGATGAAGTCTACGGCGAGTCCTCGCAACTCGAGGAGGCCCAGCTCCGCTTCCACCGCCTCAAGACCAAGTTCCACAAGGTCTTCGGACACCTTCCTCAACTCTGCGCTCGCTCTCCAGGGAGAGTGAACTTGATCGGAGAGCATATAGATTATGAGGGATACTCGGTGTTGCCGATGGCCATACGGCAGGATACTATTGTGGCGATTAGGAGGAATGAAGGAGAGAAGGTTCTGAGGATTGCGAATGTTAATGATAAGTATCAAATGTGTACTTATCCTGCTGATCCTAACCAGGAAATTGACTTGAAGAATCACAAATGGGGCCATTATTTCATCTGTGGGTACAAAGGTTTCTATGAGTTTGCCAGATCTCGAGGAATAGATTTGGGTGCACCAGTGGGACTTGATGTTCTTATTGATGGAACAGTTCCTACAGGGTCTGGACTATCAAGCTCTGCTGCATTAGTTTGTGCTTCTACAATTGCTATTATGGCTGCTTTTGAGGAGAATTTTCCCAAGAAAGAAATTGCTCAACTTACATGTGATTGTGAGCGACACATTGGTACACAGTCTGGTGGAATGGATCAGGCAATCTCGGTAATGGCTAAAACTGGGTTTGCAGAACTGATCGATTTCAACCCTATTCGTGCTACTGATGTGCAACTTCCAGCTGGTGGAACTTTTGTTGTAGCCCATTCCTTGGCAGAATCTCAGAAAGCAGTAACTGCTGCTACCAATTATAATAATAGAGTTGTTGAATGTCGTTTGGCTTCGATTCTGCTTGCTATAAAGTTGGGAATGAAGCCGCAAGATGCAATTTTAAACGTGAAAACTCTTTCTGATGTTGAAGGGTTGTGCGTATCATATGCTAGTAACCATGGGTCTTCAGATCCTAACCTGGCTGTGAAGGAGTTGTTGAAGGAAGAACCTTATACCCTTGATGAAATTGAGAAAATCACTGGGGAAAATCTAGAGTCTATCTTTCAAAATTCTGCATCGTCTCTTGATGTTTTGAAGGCTGCTAAACACTTCAAGTTATATCAGAGAGCCTCCCACGTGTACTCTGAGGCCAAGCGCGTACATGCATTCAAGGACACTGTATCATCAGATTTAAGTGACGAAGATAAGCTGAAGAATCTTGGTGATCTTATGAATGCGAGCCATTATAGCTGCAGTGTTTTATATGAGTGCAGTTGTCCGGAGTTGGAAGAACTTGTAAATATTTGTAAAGAGAACGGTGCTCTTGGGGCAAGGCTGACAGGAGCCGGATGGGGTGGCTGTGCAGTTGCTTTGGTAAAAGACAGCATTGTGCCACAGTTCATCCTTAATTTAAAGGAACAATTCTACCAGTCGAGGATTAGTAGAGGTGTCATAAACAATAACGATCTTGGCTTGTATGTTTTTGCTTCGAAGCCTTCGAGTGGCGCTGCTATTTTCAAGTTTTAG

Protein Analysis

500

Amino Acids

54.79

Weight (kDa)

5.87

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GalKase_gal_bdg PF10509 41 - 89 2.8e-22 Galactokinase galactose-binding signature
GHMP_kinases_N PF00288 144 - 220 1.5e-16 GHMP kinases N terminal domain
GHMP_kinases_C PF08544 395 - 467 2.3e-15 GHMP kinases C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014620)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06580
fragaria_vesca FvH4_4g20200 FvH4_4g20200
malus_domestica MD13G1150000.v1.1 MD16G1150400.v1.1
prunus_persica Prupe.1G114000_v2.0.a1
pyrus_communis pycom13g12920 pycom16g12880
rosa_chinensis RchiOBHm_Chr4g0425841
rosa_laevigata RLG00000007336
rosa_multiflora Rmu_sc0007063.1_g000006 Rmu_sc0009636.1_g000002
rosa_roxburghii Rroxscaffold_5G00367820
rosa_rugosa Rorug04G0206700
rosa_samantha Rh4CG282200 Rh4DG266100
rosa_wichuraiana Rw4G022770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 795
AccB7I CCANNNNNTGG 2 cut(s) 728, 754
AccI GTMKAC 1 cut(s) 63
AccII CGCG 1 cut(s) 1131
AccIII TCCGGA 1 cut(s) 1252
AciI CCGC 3 cut(s) 100, 109, 860
AclWI GGATC 4 cut(s) 28, 332, 645, 938
AcoI YGGCCR 1 cut(s) 231
AcsI RAATTY 2 cut(s) 562, 1042
AcuI CTGAAG 2 cut(s) 924, 1205
AcvI CACGTG 1 cut(s) 1111
AfaI GTAC 5 cut(s) 325, 398, 619, 1115, 1134
AfiI CCNNNNNNNGG 3 cut(s) 728, 754, 1475
AflIII ACRYGT 2 cut(s) 593, 1110
AjnI CCWGG 3 cut(s) 169, 345, 951
AloI GAACNNNNNNTCC 6 cut(s) 18, 50, 267, 299, 447, 479
AluBI AGCT 5 cut(s) 97, 507, 725, 1183, 1224
AluI AGCT 5 cut(s) 97, 507, 725, 1183, 1224
Alw21I GWGCWC 2 cut(s) 448, 1297
Alw26I GTCTC 1 cut(s) 1059
Alw44I GTGCAC 1 cut(s) 444
AlwI GGATC 4 cut(s) 28, 332, 645, 938
AlwNI CAGNNNCTG 2 cut(s) 494, 779
Ama87I CYCGRG 2 cut(s) 83, 426
Aor13HI TCCGGA 1 cut(s) 1252
AoxI GGCC 4 cut(s) 90, 231, 376, 1122
ApaLI GTGCAC 1 cut(s) 444
ApeKI GCWGC 6 cut(s) 512, 548, 782, 1073, 1224, 1481
ApoI RAATTY 2 cut(s) 562, 1042
Asp700I GAANNNNTTC 1 cut(s) 27
AspLEI GCGC 4 cut(s) 52, 161, 1131, 1481
AspS9I GGNCC 2 cut(s) 91, 376
AsuHPI GGTGA 1 cut(s) 1208
AsuII TTCGAA 1 cut(s) 1463
AvaI CYCGRG 2 cut(s) 83, 426
BaeGI GKGCMC 1 cut(s) 448
BaeI ACNNNNGTAYC 4 cut(s) 237, 270, 388, 421
BalI TGGCCA 1 cut(s) 233
BbrPI CACGTG 1 cut(s) 1111
BbsI GAAGAC 2 cut(s) 127, 930
Bbv12I GWGCWC 2 cut(s) 448, 1297
BbvI GCAGC 6 cut(s) 499, 535, 769, 1060, 1211, 1468
BccI CCATC 3 cut(s) 223, 467, 1317
BceAI ACGGC 2 cut(s) 82, 254
BciT130I CCWGG 3 cut(s) 171, 347, 953
BciVI GTATCC 2 cut(s) 206, 238
BcoDI GTCTC 1 cut(s) 1059
BfaI CTAG 2 cut(s) 924, 1025
BfmI CTRYAG 2 cut(s) 486, 1225
BfoI RGCGCY 2 cut(s) 53, 1482
BfuI GTATCC 2 cut(s) 206, 238
BglII AGATCT 1 cut(s) 422
BisI GCNGC 7 cut(s) 513, 549, 783, 860, 1074, 1225, 1482
BlsI GCNGC 7 cut(s) 514, 550, 784, 861, 1075, 1226, 1483
Bme1390I CCNGG 3 cut(s) 171, 347, 953
BmeT110I CYCGRG 2 cut(s) 83, 426
BmgT120I GGNCC 2 cut(s) 91, 376
BmiI GGNNCC 2 cut(s) 377, 1318
BmrFI CCNGG 3 cut(s) 171, 347, 953
BmrI ACTGGG 2 cut(s) 675, 1023
BmsI GCATC 2 cut(s) 856, 1058
BmuI ACTGGG 2 cut(s) 675, 1023
BpiI GAAGAC 2 cut(s) 127, 930
BplI GAGNNNNNCTC 2 cut(s) 200, 232
BpmI CTGGAG 1 cut(s) 153
Bpu14I TTCGAA 1 cut(s) 1463
BpuEI CTTGAG 1 cut(s) 98
Bsa29I ATCGAT 1 cut(s) 684
BsaAI YACGTR 1 cut(s) 1111
BsaBI GATNNNNATC 1 cut(s) 312
BsaJI CCNNGG 3 cut(s) 170, 753, 931
BsaWI WCCGGW 1 cut(s) 1252
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
Bsc4I CCNNNNNNNGG 3 cut(s) 728, 754, 1475
Bse1I ACTGG 4 cut(s) 449, 670, 1018, 1402
Bse8I GATNNNNATC 1 cut(s) 312
BseAI TCCGGA 1 cut(s) 1252
BseBI CCWGG 3 cut(s) 171, 347, 953
BseCI ATCGAT 1 cut(s) 684
BseDI CCNNGG 3 cut(s) 170, 753, 931
BseGI GGATG 2 cut(s) 1328, 1374
BseJI GATNNNNATC 1 cut(s) 312
BseLI CCNNNNNNNGG 3 cut(s) 728, 754, 1475
BseMII CTCAG 3 cut(s) 278, 779, 1110
BseNI ACTGG 4 cut(s) 449, 670, 1018, 1402
BseRI GAGGAG 2 cut(s) 101, 572
BseSI GKGCMC 1 cut(s) 448
BseXI GCAGC 6 cut(s) 499, 535, 769, 1060, 1211, 1468
BseYI CCCAGC 1 cut(s) 93
BsgI GTGCAG 2 cut(s) 1264, 1356
Bsh1236I CGCG 1 cut(s) 1131
BshFI GGCC 4 cut(s) 92, 233, 378, 1124
BshVI ATCGAT 1 cut(s) 684
BsiHKAI GWGCWC 2 cut(s) 448, 1297
BsiHKCI CYCGRG 2 cut(s) 83, 426
BsiSI CCGG 2 cut(s) 1253, 1320
BslFI GGGAC 1 cut(s) 468
BslI CCNNNNNNNGG 3 cut(s) 728, 754, 1475
BsmAI GTCTC 1 cut(s) 1059
BsmBI CGTCTC 1 cut(s) 1059
BsmFI GGGAC 1 cut(s) 468
BsmI GAATGC 2 cut(s) 1139, 1213
BsnI GGCC 4 cut(s) 92, 233, 378, 1124
BsoBI CYCGRG 2 cut(s) 83, 426
Bsp119I TTCGAA 1 cut(s) 1463
Bsp1286I GDGCHC 2 cut(s) 448, 1297
Bsp13I TCCGGA 1 cut(s) 1252
Bsp143I GATC 9 cut(s) 33, 186, 337, 422, 637, 681, 943, 1198, 1438
Bsp19I CCATGG 1 cut(s) 931
BspACI CCGC 3 cut(s) 100, 109, 860
BspANI GGCC 4 cut(s) 92, 233, 378, 1124
BspCNI CTCAG 3 cut(s) 279, 778, 1111
BspDI ATCGAT 1 cut(s) 684
BspEI TCCGGA 1 cut(s) 1252
BspFNI CGCG 1 cut(s) 1131
BspLI GGNNCC 2 cut(s) 377, 1318
BspMAI CTGCAG 1 cut(s) 1229
BspPI GGATC 4 cut(s) 28, 332, 645, 938
BspT104I TTCGAA 1 cut(s) 1463
BsrI ACTGG 4 cut(s) 449, 670, 1018, 1402
BssECI CCNNGG 3 cut(s) 170, 753, 931
BssMI GATC 9 cut(s) 33, 186, 337, 422, 637, 681, 943, 1198, 1438
BssT1I CCWWGG 2 cut(s) 753, 931
Bst2UI CCWGG 3 cut(s) 171, 347, 953
Bst4CI ACNGT 5 cut(s) 481, 624, 1153, 1292, 1371
BstAPI GCANNNNNTGC 1 cut(s) 779
BstBAI YACGTR 1 cut(s) 1111
BstBI TTCGAA 1 cut(s) 1463
BstC8I GCNNGC 3 cut(s) 163, 837, 1213
BstDEI CTNAG 3 cut(s) 287, 765, 1119
BstDSI CCRYGG 1 cut(s) 931
BstF5I GGATG 2 cut(s) 1328, 1374
BstFNI CGCG 1 cut(s) 1131
BstH2I RGCGCY 2 cut(s) 53, 1482
BstHHI GCGC 4 cut(s) 52, 161, 1131, 1481
BstKTI GATC 9 cut(s) 36, 189, 340, 425, 640, 684, 946, 1201, 1441
BstMAI GTCTC 1 cut(s) 1059
BstMBI GATC 9 cut(s) 33, 186, 337, 422, 637, 681, 943, 1198, 1438
BstMWI GCNNNNNNNGC 5 cut(s) 108, 545, 779, 1221, 1301
BstNI CCWGG 3 cut(s) 171, 347, 953
BstNSI RCATGY 2 cut(s) 597, 1139
BstSCI CCNGG 3 cut(s) 169, 345, 951
BstSFI CTRYAG 2 cut(s) 486, 1225
BstSLI GKGCMC 1 cut(s) 448
BstUI CGCG 1 cut(s) 1131
BstV1I GCAGC 6 cut(s) 499, 535, 769, 1060, 1211, 1468
BstV2I GAAGAC 2 cut(s) 127, 930
BstX2I RGATCY 2 cut(s) 422, 943
BstYI RGATCY 2 cut(s) 422, 943
Bsu15I ATCGAT 1 cut(s) 684
BsuI GTATCC 2 cut(s) 206, 238
BsuRI GGCC 4 cut(s) 92, 233, 378, 1124
BsuTUI ATCGAT 1 cut(s) 684
BtgI CCRYGG 1 cut(s) 931
BtsCI GGATG 2 cut(s) 1328, 1374
BtsI GCAGTG 1 cut(s) 1234
BtsIMutI CAGTG 4 cut(s) 456, 1011, 1149, 1234
Cac8I GCNNGC 3 cut(s) 163, 837, 1213
CaiI CAGNNNCTG 2 cut(s) 494, 779
CfoI GCGC 4 cut(s) 52, 161, 1131, 1481
Cfr13I GGNCC 2 cut(s) 91, 376
ClaI ATCGAT 1 cut(s) 684
Csp6I GTAC 5 cut(s) 324, 397, 618, 1114, 1133
CviAII CATG 3 cut(s) 594, 932, 1136
CviQI GTAC 5 cut(s) 324, 397, 618, 1114, 1133
DdeI CTNAG 3 cut(s) 287, 765, 1119
DpnI GATC 9 cut(s) 35, 188, 339, 424, 639, 683, 945, 1200, 1440
DpnII GATC 9 cut(s) 33, 186, 337, 422, 637, 681, 943, 1198, 1438
DraI TTTAAA 2 cut(s) 876, 1386
EaeI YGGCCR 1 cut(s) 231
Eco130I CCWWGG 2 cut(s) 753, 931
Eco57I CTGAAG 2 cut(s) 924, 1205
Eco72I CACGTG 1 cut(s) 1111
Eco88I CYCGRG 2 cut(s) 83, 426
EcoRII CCWGG 3 cut(s) 169, 345, 951
EcoT14I CCWWGG 2 cut(s) 753, 931
EcoT22I ATGCAT 1 cut(s) 1141
ErhI CCWWGG 2 cut(s) 753, 931
Esp3I CGTCTC 1 cut(s) 1059
FaeI CATG 3 cut(s) 597, 935, 1139
FaqI GGGAC 1 cut(s) 468
FatI CATG 3 cut(s) 593, 931, 1135
FauNDI CATATG 1 cut(s) 919
FblI GTMKAC 1 cut(s) 63
Fnu4HI GCNGC 7 cut(s) 513, 549, 783, 860, 1074, 1225, 1482
FokI GGATG 2 cut(s) 1335, 1361
Fsp4HI GCNGC 7 cut(s) 513, 549, 783, 860, 1074, 1225, 1482
FspBI CTAG 2 cut(s) 924, 1025
GlaI GCGC 4 cut(s) 51, 160, 1130, 1480
GluI GCNGC 7 cut(s) 513, 549, 783, 860, 1074, 1225, 1482
GsaI CCCAGC 1 cut(s) 97
GsuI CTGGAG 1 cut(s) 153
HaeII RGCGCY 2 cut(s) 53, 1482
HaeIII GGCC 4 cut(s) 92, 233, 378, 1124
HapII CCGG 2 cut(s) 1253, 1320
HhaI GCGC 4 cut(s) 52, 161, 1131, 1481
Hin1II CATG 3 cut(s) 597, 935, 1139
Hin6I GCGC 4 cut(s) 50, 159, 1129, 1479
HinP1I GCGC 4 cut(s) 50, 159, 1129, 1479
HinfI GANTC 6 cut(s) 71, 364, 761, 829, 1028, 1189
HpaII CCGG 2 cut(s) 1253, 1320
HphI GGTGA 1 cut(s) 1208
Hpy166II GTNNAC 6 cut(s) 64, 181, 324, 446, 620, 1114
Hpy188III TCNNGA 6 cut(s) 115, 426, 495, 1025, 1058, 1253
Hpy8I GTNNAC 6 cut(s) 64, 181, 324, 446, 620, 1114
HpyAV CCTTC 8 cut(s) 155, 266, 274, 896, 955, 967, 1063, 1479
HpyCH4III ACNGT 5 cut(s) 481, 624, 1153, 1292, 1371
HpyCH4IV ACGT 2 cut(s) 879, 1110
HpyF10VI GCNNNNNNNGC 5 cut(s) 108, 545, 779, 1221, 1301
HpyF3I CTNAG 3 cut(s) 287, 765, 1119
HpySE526I ACGT 2 cut(s) 879, 1110
Hsp92II CATG 3 cut(s) 597, 935, 1139
HspAI GCGC 4 cut(s) 50, 159, 1129, 1479
Kpn2I TCCGGA 1 cut(s) 1252
Kzo9I GATC 9 cut(s) 33, 186, 337, 422, 637, 681, 943, 1198, 1438
LmnI GCTCC 2 cut(s) 102, 1316
Lsp1109I GCAGC 6 cut(s) 499, 535, 769, 1060, 1211, 1468
LweI GCATC 2 cut(s) 856, 1058
MaeI CTAG 2 cut(s) 924, 1025
MaeII ACGT 2 cut(s) 879, 1110
MaeIII GTNAC 3 cut(s) 775, 926, 1169
MalI GATC 9 cut(s) 35, 188, 339, 424, 639, 683, 945, 1200, 1440
MboI GATC 9 cut(s) 33, 186, 337, 422, 637, 681, 943, 1198, 1438
MboII GAAGA 8 cut(s) 35, 127, 373, 930, 989, 1187, 1198, 1274
MfeI CAATTG 1 cut(s) 534
MflI RGATCY 2 cut(s) 422, 943
MhlI GDGCHC 2 cut(s) 448, 1297
MlsI TGGCCA 1 cut(s) 233
MluNI TGGCCA 1 cut(s) 233
MlyI GAGTC 2 cut(s) 80, 1037
MmeI TCCRAC 1 cut(s) 1239
Mox20I TGGCCA 1 cut(s) 233
Mph1103I ATGCAT 1 cut(s) 1141
MroI TCCGGA 1 cut(s) 1252
MroXI GAANNNNTTC 1 cut(s) 27
MscI TGGCCA 1 cut(s) 233
MseI TTAA 5 cut(s) 303, 875, 1166, 1380, 1385
Msp20I TGGCCA 1 cut(s) 233
MspA1I CMGCKG 1 cut(s) 725
MspI CCGG 2 cut(s) 1253, 1320
MspR9I CCNGG 3 cut(s) 171, 347, 953
MunI CAATTG 1 cut(s) 534
Mva1269I GAATGC 2 cut(s) 1139, 1213
MvaI CCWGG 3 cut(s) 171, 347, 953
MvnI CGCG 1 cut(s) 1131
MwoI GCNNNNNNNGC 5 cut(s) 108, 545, 779, 1221, 1301
NcoI CCATGG 1 cut(s) 931
NdeI CATATG 1 cut(s) 919
NdeII GATC 9 cut(s) 33, 186, 337, 422, 637, 681, 943, 1198, 1438
NlaIII CATG 3 cut(s) 597, 935, 1139
NlaIV GGNNCC 2 cut(s) 377, 1318
NmeAIII GCCGAG 1 cut(s) 26
NmuCI GTSAC 1 cut(s) 1169
NsiI ATGCAT 1 cut(s) 1141
NspI RCATGY 2 cut(s) 597, 1139
NspV TTCGAA 1 cut(s) 1463
PaeR7I CTCGAG 2 cut(s) 83, 426
PciI ACATGT 1 cut(s) 593
PcsI WCGNNNNNNNCGW 2 cut(s) 224, 1469
PctI GAATGC 2 cut(s) 1139, 1213
PdmI GAANNNNTTC 1 cut(s) 27
PfeI GAWTC 4 cut(s) 364, 761, 829, 1189
PflMI CCANNNNNTGG 2 cut(s) 728, 754
PkrI GCNGC 7 cut(s) 514, 550, 784, 861, 1075, 1226, 1483
PleI GAGTC 2 cut(s) 79, 1036
PmaCI CACGTG 1 cut(s) 1111
PmlI CACGTG 1 cut(s) 1111
PpsI GAGTC 2 cut(s) 79, 1036
Ppu21I YACGTR 1 cut(s) 1111
PscI ACATGT 1 cut(s) 593
PsiI TTATAA 1 cut(s) 795
Psp6I CCWGG 3 cut(s) 169, 345, 951
PspCI CACGTG 1 cut(s) 1111
PspFI CCCAGC 1 cut(s) 93
PspGI CCWGG 3 cut(s) 169, 345, 951
PspN4I GGNNCC 2 cut(s) 377, 1318
PspPI GGNCC 2 cut(s) 91, 376
PspXI VCTCGAGB 1 cut(s) 83
PsrI GAACNNNNNNTAC 2 cut(s) 469, 501
PstI CTGCAG 1 cut(s) 1229
PstNI CAGNNNCTG 2 cut(s) 494, 779
PsuI RGATCY 2 cut(s) 422, 943
PvuII CAGCTG 1 cut(s) 725
RsaI GTAC 5 cut(s) 325, 398, 619, 1115, 1134
RsaNI GTAC 5 cut(s) 324, 397, 618, 1114, 1133
SaqAI TTAA 5 cut(s) 303, 875, 1166, 1380, 1385
SatI GCNGC 7 cut(s) 513, 549, 783, 860, 1074, 1225, 1482
Sau3AI GATC 9 cut(s) 33, 186, 337, 422, 637, 681, 943, 1198, 1438
Sau96I GGNCC 2 cut(s) 91, 376
SchI GAGTC 2 cut(s) 80, 1037
ScrFI CCNGG 3 cut(s) 171, 347, 953
SduI GDGCHC 2 cut(s) 448, 1297
SfaNI GCATC 2 cut(s) 856, 1058
SfcI CTRYAG 2 cut(s) 486, 1225
Sfr274I CTCGAG 2 cut(s) 83, 426
SfuI TTCGAA 1 cut(s) 1463
SlaI CTCGAG 2 cut(s) 83, 426
SmlI CTYRAG 3 cut(s) 83, 113, 426
SmoI CTYRAG 3 cut(s) 83, 113, 426
SsiI CCGC 3 cut(s) 100, 109, 860
SspI AATATT 1 cut(s) 1276
SspMI CTAG 2 cut(s) 924, 1025
StyD4I CCNGG 3 cut(s) 169, 345, 951
StyI CCWWGG 2 cut(s) 753, 931
TaaI ACNGT 5 cut(s) 481, 624, 1153, 1292, 1371
TaiI ACGT 2 cut(s) 882, 1113
TaqI TCGA 8 cut(s) 54, 84, 427, 684, 827, 1406, 1463, 1472
TatI WGTACW 2 cut(s) 323, 1113
TauI GCSGC 1 cut(s) 862
TfiI GAWTC 4 cut(s) 364, 761, 829, 1189
Tru1I TTAA 5 cut(s) 303, 875, 1166, 1380, 1385
Tru9I TTAA 5 cut(s) 303, 875, 1166, 1380, 1385
TscAI CASTG 4 cut(s) 456, 1018, 1156, 1234
TseFI GTSAC 1 cut(s) 1169
TseI GCWGC 6 cut(s) 512, 548, 782, 1073, 1224, 1481
Tsp45I GTSAC 1 cut(s) 1169
TspDTI ATGAA 7 cut(s) 72, 285, 376, 869, 1011, 1220, 1363
TspRI CASTG 4 cut(s) 456, 1018, 1156, 1234
Van91I CCANNNNNTGG 2 cut(s) 728, 754
VneI GTGCAC 1 cut(s) 444
XapI RAATTY 2 cut(s) 562, 1042
XbaI TCTAGA 1 cut(s) 1024
XceI RCATGY 2 cut(s) 597, 1139
XhoI CTCGAG 2 cut(s) 83, 426
XmiI GTMKAC 1 cut(s) 63
XmnI GAANNNNTTC 1 cut(s) 27
XspI CTAG 2 cut(s) 924, 1025
Zsp2I ATGCAT 1 cut(s) 1141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.