pycom13g12920

gal1,galk

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0014620
Physical Location & Seq
Reverse (-)
9099889 .. 9103084
3196 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g12920.12

Sequence Viewer

Length: 1509 bp
ATGGCACAAATGGCAAAACACGAAGAACTTCCGATCCCAATCCACGACACCCTCGACGCCGTCTACGGCAACTCCTCCCAACTCGACGAGGCCCAGCTCCGATTCAACCACCTGAACTCCAAGTTCCAGCAGGTTTTCGGTCACCCCCCTCAACTCTTCGCTCGCTCTCCAGGGAGGGTGAACTTGGTCGGAGAGCACATAGACTACGAGGGATACTCGGTGCTGCCGATGGCCATACGACAGGACACCATTGTCGCAATTCGGAAGCACGACGACGGAGACGCCGAGAAAGTTCTCAGAATTGCCAATGTCAACGACAAGTACCAAATTTGTACTTACCCCGCCGACCCGACCCAGGAAATTGACTTGAAGAATCACAAATGGGGGCATTATTTCATTTGTGGGTACAAAGGTTTTTATGAGTTTGCCAAATCCAAGGGAGAAAATGTTGGACCAGTTGGACTTGATGTTCTTATTGATGGAACAGTTCCTACAGGCTCTGGACTATCTAGCTCTGCTGCATTAGTTTGTGCTTCTACGATTGCGATAATGGCCGCTTTTGGTTTGAATTTCCCCAAGAAAGAAATTGCACAACTTACGTGTGAGTGTGAGCGACACATTGGCACACAATCTGGTGGAATGGATCAGGCAATCTCGGTGATGGCCAAGCCTGGGTTTGCAGAACTAATTGATTTCAACCCAATTCGTGCTACTGATGTGCAACTTCCGGCTGGTGGAACTTTTGTTGTGGCCCATTCTTTGGCAGATTCTCAGAAAGCAGTAACTGCTGCCACAAATTACAATAACCGAGTTGTTGAGTGTCGCCTGGCTGCTATTGTGCTTGGTATAAAGTTGGGGATGAAACCGCAAGAGGCAATTTCAAGTGTCAAAACTCTTTCTGATGTTGAAGGCTTATGCATATCATTTGCTAGTACTCGTGGGTCTTCAGATCCGATCGTGGCTGTAAAGGAGAATTTAAAAGAAGAACCTTATACAGCTGGGGAAATAGAGAAAATCCTTGGGGAGAGTCTACCCTCAATCTTGAGAGATTCTCCGTCTTCTTTGGATGTTTTGAAAGCTGCAAAGCACTTCAAGTTATCCCAGAGAGCCGCCCATGTGTATTCTGAAGCCAAGCGGGTATATGCTTTCAAGGATGCTGTATCATCAACAGTAAGTGATGAGGATAAGCTAAAGAGGCTTGGTGATCTTATGAATGATAGCCACTATAGCTGCAGCGTGTTATATGAGTGCAGCTGTCCAGAGTTGGAAGAACTTGTAAATATTTGTCGGGAGAATGGTGCTCTTGGGGCAAGGCTTACGGGAGCTGGATGGGGCGGCTGTGCAGTTGCTTTGGTAAAAGATAGTATTGTCCCACAATTTATCCTCAATCTAAAGGAACGCTTCTACCAGTCACGAATTGACAGAGGCGTCATCAACAACAACGATCTTGGCCTCTATGTTTTTGCGTCCAAGCCCTCCAGCGGTGCTGCTATCGTCAAGTTTGTCTGA

Protein Analysis

503

Amino Acids

54.52

Weight (kDa)

5.94

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014620)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06580
fragaria_vesca FvH4_4g20200 FvH4_4g20200
malus_domestica MD13G1150000.v1.1 MD16G1150400.v1.1
prunus_persica Prupe.1G114000_v2.0.a1
pyrus_communis pycom13g12920 pycom16g12880
rosa_chinensis RchiOBHm_Chr4g0425841
rosa_laevigata RLG00000007336
rosa_multiflora Rmu_sc0007063.1_g000006 Rmu_sc0009636.1_g000002
rosa_roxburghii Rroxscaffold_5G00367820
rosa_rugosa Rorug04G0206700
rosa_samantha Rh4CG282200 Rh4DG266100
rosa_wichuraiana Rw4G022770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 251, 1427
Acc36I ACCTGC 1 cut(s) 121
AccB7I CCANNNNNTGG 1 cut(s) 760
AccI GTMKAC 2 cut(s) 63, 1030
AciI CCGC 7 cut(s) 342, 555, 866, 1110, 1135, 1335, 1482
AclWI GGATC 3 cut(s) 28, 651, 944
AcoI YGGCCR 3 cut(s) 231, 552, 663
AcsI RAATTY 3 cut(s) 327, 568, 973
AcuI CTGAAG 2 cut(s) 930, 1146
AcyI GRCGYC 3 cut(s) 57, 282, 1428
AfaI GTAC 4 cut(s) 323, 334, 407, 934
AfiI CCNNNNNNNGG 5 cut(s) 355, 672, 734, 760, 1481
AflIII ACRYGT 1 cut(s) 599
AgsI TTSAA 9 cut(s) 106, 370, 568, 697, 882, 908, 1075, 1093, 1150
AjnI CCWGG 4 cut(s) 169, 354, 670, 825
AloI GAACNNNNNNTCC 4 cut(s) 18, 50, 453, 485
AluBI AGCT 8 cut(s) 97, 513, 998, 1079, 1189, 1230, 1254, 1325
AluI AGCT 8 cut(s) 97, 513, 998, 1079, 1189, 1230, 1254, 1325
Alw21I GWGCWC 2 cut(s) 198, 1303
Alw26I GTCTC 1 cut(s) 273
AlwI GGATC 3 cut(s) 28, 651, 944
AlwNI CAGNNNCTG 2 cut(s) 500, 785
AoxI GGCC 6 cut(s) 90, 231, 552, 663, 750, 1450
ApeKI GCWGC 9 cut(s) 223, 518, 788, 830, 1079, 1230, 1233, 1251, 1487
ApoI RAATTY 3 cut(s) 327, 568, 973
Asp700I GAANNNNTTC 1 cut(s) 27
AspS9I GGNCC 3 cut(s) 91, 452, 751
AsuHPI GGTGA 4 cut(s) 134, 190, 670, 1214
AvaII GGWCC 1 cut(s) 452
BaeI ACNNNNGTAYC 2 cut(s) 397, 430
BalI TGGCCA 2 cut(s) 233, 665
BauI CACGAG 1 cut(s) 936
BbsI GAAGAC 2 cut(s) 936, 1050
Bbv12I GWGCWC 2 cut(s) 198, 1303
BbvI GCAGC 9 cut(s) 210, 505, 775, 817, 1066, 1217, 1245, 1263, 1474
BccI CCATC 4 cut(s) 223, 473, 655, 1323
BceAI ACGGC 2 cut(s) 44, 82
BciT130I CCWGG 4 cut(s) 171, 356, 672, 827
BciVI GTATCC 1 cut(s) 206
BcoDI GTCTC 1 cut(s) 273
BfaI CTAG 2 cut(s) 510, 930
BfmI CTRYAG 3 cut(s) 492, 1225, 1231
BfuAI ACCTGC 1 cut(s) 121
BfuI GTATCC 1 cut(s) 206
BmcAI AGTACT 1 cut(s) 934
Bme1390I CCNGG 4 cut(s) 171, 356, 672, 827
Bme18I GGWCC 1 cut(s) 452
BmgT120I GGNCC 3 cut(s) 91, 452, 751
BmrFI CCNGG 4 cut(s) 171, 356, 672, 827
BmsI GCATC 1 cut(s) 1144
BpiI GAAGAC 2 cut(s) 936, 1050
BplI GAGNNNNNCTC 4 cut(s) 200, 232, 1036, 1068
BpmI CTGGAG 2 cut(s) 153, 1462
BpuEI CTTGAG 1 cut(s) 1063
BsaAI YACGTR 1 cut(s) 600
BsaBI GATNNNNATC 1 cut(s) 38
BsaHI GRCGYC 3 cut(s) 57, 282, 1428
BsaJI CCNNGG 5 cut(s) 170, 354, 435, 671, 1018
BsaXI ACNNNNNCTCC 6 cut(s) 56, 86, 101, 131, 432, 462
Bsc4I CCNNNNNNNGG 5 cut(s) 355, 672, 734, 760, 1481
Bse1I ACTGG 2 cut(s) 455, 1408
Bse8I GATNNNNATC 1 cut(s) 38
BseBI CCWGG 4 cut(s) 171, 356, 672, 827
BseDI CCNNGG 5 cut(s) 170, 354, 435, 671, 1018
BseGI GGATG 4 cut(s) 864, 1072, 1159, 1334
BseJI GATNNNNATC 1 cut(s) 38
BseLI CCNNNNNNNGG 5 cut(s) 355, 672, 734, 760, 1481
BseMII CTCAG 2 cut(s) 310, 785
BseNI ACTGG 2 cut(s) 455, 1408
BseRI GAGGAG 1 cut(s) 64
BseXI GCAGC 9 cut(s) 210, 505, 775, 817, 1066, 1217, 1245, 1263, 1474
BseYI CCCAGC 2 cut(s) 93, 998
BsgI GTGCAG 2 cut(s) 1270, 1362
Bsh1285I CGRYCG 1 cut(s) 957
BshFI GGCC 6 cut(s) 92, 233, 554, 665, 752, 1452
BsiEI CGRYCG 1 cut(s) 957
BsiHKAI GWGCWC 2 cut(s) 198, 1303
BsiSI CCGG 1 cut(s) 728
BslFI GGGAC 1 cut(s) 1355
BslI CCNNNNNNNGG 5 cut(s) 355, 672, 734, 760, 1481
BsmAI GTCTC 1 cut(s) 273
BsmBI CGTCTC 1 cut(s) 273
BsmFI GGGAC 1 cut(s) 1355
BsnI GGCC 6 cut(s) 92, 233, 554, 665, 752, 1452
Bsp1286I GDGCHC 2 cut(s) 198, 1303
Bsp143I GATC 6 cut(s) 33, 643, 949, 954, 1204, 1444
BspACI CCGC 7 cut(s) 342, 555, 866, 1110, 1135, 1335, 1482
BspANI GGCC 6 cut(s) 92, 233, 554, 665, 752, 1452
BspCNI CTCAG 2 cut(s) 309, 784
BspMAI CTGCAG 1 cut(s) 1235
BspMI ACCTGC 1 cut(s) 121
BspPI GGATC 3 cut(s) 28, 651, 944
BsrI ACTGG 2 cut(s) 455, 1408
BssECI CCNNGG 5 cut(s) 170, 354, 435, 671, 1018
BssMI GATC 6 cut(s) 33, 643, 949, 954, 1204, 1444
BssNI GRCGYC 3 cut(s) 57, 282, 1428
BssSI CACGAG 1 cut(s) 936
BssT1I CCWWGG 2 cut(s) 435, 1018
Bst2BI CACGAG 1 cut(s) 936
Bst2UI CCWGG 4 cut(s) 171, 356, 672, 827
Bst4CI ACNGT 2 cut(s) 487, 1171
Bst6I CTCTTC 1 cut(s) 161
BstACI GRCGYC 3 cut(s) 57, 282, 1428
BstAPI GCANNNNNTGC 1 cut(s) 785
BstBAI YACGTR 1 cut(s) 600
BstC8I GCNNGC 1 cut(s) 163
BstDEI CTNAG 2 cut(s) 296, 771
BstEII GGTNACC 1 cut(s) 140
BstF5I GGATG 4 cut(s) 864, 1072, 1159, 1334
BstKTI GATC 6 cut(s) 36, 646, 952, 957, 1207, 1447
BstMAI GTCTC 1 cut(s) 273
BstMBI GATC 6 cut(s) 33, 643, 949, 954, 1204, 1444
BstMCI CGRYCG 1 cut(s) 957
BstMWI GCNNNNNNNGC 6 cut(s) 11, 551, 785, 1195, 1227, 1307
BstNI CCWGG 4 cut(s) 171, 356, 672, 827
BstPI GGTNACC 1 cut(s) 140
BstSCI CCNGG 4 cut(s) 169, 354, 670, 825
BstSFI CTRYAG 3 cut(s) 492, 1225, 1231
BstV1I GCAGC 9 cut(s) 210, 505, 775, 817, 1066, 1217, 1245, 1263, 1474
BstV2I GAAGAC 2 cut(s) 936, 1050
BstX2I RGATCY 1 cut(s) 949
BstYI RGATCY 1 cut(s) 949
BsuI GTATCC 1 cut(s) 206
BsuRI GGCC 6 cut(s) 92, 233, 554, 665, 752, 1452
BtsCI GGATG 4 cut(s) 864, 1072, 1159, 1334
BveI ACCTGC 1 cut(s) 121
Cac8I GCNNGC 1 cut(s) 163
CaiI CAGNNNCTG 2 cut(s) 500, 785
Cfr13I GGNCC 3 cut(s) 91, 452, 751
CseI GACGC 4 cut(s) 65, 290, 1417, 1455
Csp6I GTAC 4 cut(s) 322, 333, 406, 933
CviAII CATG 1 cut(s) 1115
CviQI GTAC 4 cut(s) 322, 333, 406, 933
DdeI CTNAG 2 cut(s) 296, 771
DpnI GATC 6 cut(s) 35, 645, 951, 956, 1206, 1446
DpnII GATC 6 cut(s) 33, 643, 949, 954, 1204, 1444
DraI TTTAAA 1 cut(s) 978
DrdI GACNNNNNNGTC 2 cut(s) 251, 1427
DseDI GACNNNNNNGTC 2 cut(s) 251, 1427
EaeI YGGCCR 3 cut(s) 231, 552, 663
Eam1104I CTCTTC 1 cut(s) 161
EarI CTCTTC 1 cut(s) 161
Eco130I CCWWGG 2 cut(s) 435, 1018
Eco47I GGWCC 1 cut(s) 452
Eco57I CTGAAG 2 cut(s) 930, 1146
Eco91I GGTNACC 1 cut(s) 140
EcoO65I GGTNACC 1 cut(s) 140
EcoRII CCWGG 4 cut(s) 169, 354, 670, 825
EcoT14I CCWWGG 2 cut(s) 435, 1018
EcoT22I ATGCAT 1 cut(s) 920
ErhI CCWWGG 2 cut(s) 435, 1018
Esp3I CGTCTC 1 cut(s) 273
FaeI CATG 1 cut(s) 1118
FalI AAGNNNNNCTT 2 cut(s) 1385, 1417
FaqI GGGAC 1 cut(s) 1355
FatI CATG 1 cut(s) 1114
FauI CCCGC 2 cut(s) 349, 1128
FblI GTMKAC 2 cut(s) 63, 1030
FokI GGATG 4 cut(s) 871, 1079, 1166, 1341
FspBI CTAG 2 cut(s) 510, 930
GsaI CCCAGC 2 cut(s) 97, 1002
GsuI CTGGAG 2 cut(s) 153, 1462
HaeIII GGCC 6 cut(s) 92, 233, 554, 665, 752, 1452
HapII CCGG 1 cut(s) 728
HgaI GACGC 4 cut(s) 65, 290, 1417, 1455
Hin1I GRCGYC 3 cut(s) 57, 282, 1428
Hin1II CATG 1 cut(s) 1118
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HinfI GANTC 5 cut(s) 102, 373, 767, 1027, 1049
HpaII CCGG 1 cut(s) 728
HphI GGTGA 4 cut(s) 134, 190, 670, 1214
Hpy166II GTNNAC 4 cut(s) 64, 181, 313, 1031
Hpy188III TCNNGA 5 cut(s) 501, 1042, 1259, 1289, 1413
Hpy8I GTNNAC 4 cut(s) 64, 181, 313, 1031
Hpy99I CGWCG 4 cut(s) 59, 89, 275, 278
HpyAV CCTTC 1 cut(s) 902
HpyCH4III ACNGT 2 cut(s) 487, 1171
HpyCH4IV ACGT 1 cut(s) 599
HpyCH4V TGCA 9 cut(s) 521, 590, 680, 721, 918, 1082, 1233, 1251, 1343
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 551, 785, 1195, 1227, 1307
HpyF3I CTNAG 2 cut(s) 296, 771
HpySE526I ACGT 1 cut(s) 599
Hsp92I GRCGYC 3 cut(s) 57, 282, 1428
Hsp92II CATG 1 cut(s) 1118
Kzo9I GATC 6 cut(s) 33, 643, 949, 954, 1204, 1444
LmnI GCTCC 2 cut(s) 102, 1322
Lsp1109I GCAGC 9 cut(s) 210, 505, 775, 817, 1066, 1217, 1245, 1263, 1474
LweI GCATC 1 cut(s) 1144
MaeI CTAG 2 cut(s) 510, 930
MaeII ACGT 1 cut(s) 599
MaeIII GTNAC 3 cut(s) 140, 781, 1410
MalI GATC 6 cut(s) 35, 645, 951, 956, 1206, 1446
MboI GATC 6 cut(s) 33, 643, 949, 954, 1204, 1444
MboII GAAGA 7 cut(s) 35, 148, 382, 936, 995, 1050, 1280
MflI RGATCY 1 cut(s) 949
MhlI GDGCHC 2 cut(s) 198, 1303
MlsI TGGCCA 2 cut(s) 233, 665
MluNI TGGCCA 2 cut(s) 233, 665
MlyI GAGTC 1 cut(s) 1036
MmeI TCCRAC 4 cut(s) 169, 430, 439, 1245
Mox20I TGGCCA 2 cut(s) 233, 665
Mph1103I ATGCAT 1 cut(s) 920
MroXI GAANNNNTTC 1 cut(s) 27
MscI TGGCCA 2 cut(s) 233, 665
MseI TTAA 1 cut(s) 977
Msp20I TGGCCA 2 cut(s) 233, 665
MspA1I CMGCKG 3 cut(s) 998, 1254, 1482
MspI CCGG 1 cut(s) 728
MspR9I CCNGG 4 cut(s) 171, 356, 672, 827
MvaI CCWGG 4 cut(s) 171, 356, 672, 827
MwoI GCNNNNNNNGC 6 cut(s) 11, 551, 785, 1195, 1227, 1307
NdeII GATC 6 cut(s) 33, 643, 949, 954, 1204, 1444
NlaIII CATG 1 cut(s) 1118
NmeAIII GCCGAG 1 cut(s) 310
NmuCI GTSAC 2 cut(s) 140, 1410
NsiI ATGCAT 1 cut(s) 920
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 224, 282
PdmI GAANNNNTTC 1 cut(s) 27
PfeI GAWTC 4 cut(s) 102, 373, 767, 1049
PflFI GACNNNGTC 1 cut(s) 59
PflMI CCANNNNNTGG 1 cut(s) 760
Ple19I CGATCG 1 cut(s) 957
PleI GAGTC 1 cut(s) 1035
PpsI GAGTC 1 cut(s) 1035
Ppu21I YACGTR 1 cut(s) 600
Psp6I CCWGG 4 cut(s) 169, 354, 670, 825
PspEI GGTNACC 1 cut(s) 140
PspFI CCCAGC 2 cut(s) 93, 998
PspGI CCWGG 4 cut(s) 169, 354, 670, 825
PspPI GGNCC 3 cut(s) 91, 452, 751
PsrI GAACNNNNNNTAC 2 cut(s) 475, 507
PstI CTGCAG 1 cut(s) 1235
PstNI CAGNNNCTG 2 cut(s) 500, 785
PsuI RGATCY 1 cut(s) 949
PsyI GACNNNGTC 1 cut(s) 59
PvuI CGATCG 1 cut(s) 957
PvuII CAGCTG 2 cut(s) 998, 1254
RsaI GTAC 4 cut(s) 323, 334, 407, 934
RsaNI GTAC 4 cut(s) 322, 333, 406, 933
SaqAI TTAA 1 cut(s) 977
Sau3AI GATC 6 cut(s) 33, 643, 949, 954, 1204, 1444
Sau96I GGNCC 3 cut(s) 91, 452, 751
ScaI AGTACT 1 cut(s) 934
SchI GAGTC 1 cut(s) 1036
ScrFI CCNGG 4 cut(s) 171, 356, 672, 827
SduI GDGCHC 2 cut(s) 198, 1303
SfaNI GCATC 1 cut(s) 1144
SfcI CTRYAG 3 cut(s) 492, 1225, 1231
SinI GGWCC 1 cut(s) 452
SmlI CTYRAG 1 cut(s) 1042
SmoI CTYRAG 1 cut(s) 1042
SsiI CCGC 7 cut(s) 342, 555, 866, 1110, 1135, 1335, 1482
SspI AATATT 1 cut(s) 1282
SspMI CTAG 2 cut(s) 510, 930
StyD4I CCNGG 4 cut(s) 169, 354, 670, 825
StyI CCWWGG 2 cut(s) 435, 1018
TaaI ACNGT 2 cut(s) 487, 1171
TaiI ACGT 1 cut(s) 602
TaqI TCGA 2 cut(s) 54, 84
TaqII GACCGA 1 cut(s) 128
TatI WGTACW 2 cut(s) 332, 932
TauI GCSGC 3 cut(s) 557, 1112, 1338
TfiI GAWTC 4 cut(s) 102, 373, 767, 1049
Tru1I TTAA 1 cut(s) 977
Tru9I TTAA 1 cut(s) 977
TseFI GTSAC 2 cut(s) 140, 1410
TseI GCWGC 9 cut(s) 223, 518, 788, 830, 1079, 1230, 1233, 1251, 1487
Tsp45I GTSAC 2 cut(s) 140, 1410
TspDTI ATGAA 3 cut(s) 385, 875, 1226
TspGWI ACGGA 2 cut(s) 291, 1044
Tth111I GACNNNGTC 1 cut(s) 59
Van91I CCANNNNNTGG 1 cut(s) 760
VpaK11BI GGWCC 1 cut(s) 452
XapI RAATTY 3 cut(s) 327, 568, 973
XmiI GTMKAC 2 cut(s) 63, 1030
XmnI GAANNNNTTC 1 cut(s) 27
XspI CTAG 2 cut(s) 510, 930
ZrmI AGTACT 1 cut(s) 934
Zsp2I ATGCAT 1 cut(s) 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.