Rorug04G0206700

gal1,galk

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0014620
Physical Location & Seq
Reverse (-)
35994343 .. 35998322
3980 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0206700.1

Sequence Viewer

Length: 558 bp
ATGGCGTCGTCCCGGTCTTCACTCTACCGCACCCTCCGCTCCTTCTCCACCACCACCAGACCCTCCCACCACAACAGCCACCACCACACTCACCGCTACACCGAGCCGAGCTCCTTCATCGGAAGCTGGGACGCGCCGAGCAACCCCAAGGAGGCGCAGGCCAAGCTCGCGCATCTCCGGCGAGACTACGCGAAGCAGGTCAAGGAGATCCGCAAGGAGTACATCAAGGAGGTCGAGCTGATGAGGCTCGAGAAGCTCCGAAAGGACGAGGCCCGTAAAGAAGCCCTCAGGCTTCAGAACGAGGAGCGCAAGAGGCTCAAGGCCGAGGCCGCTAAGGTCCGGGCCCAACAGCGCCAGGTCGCCGAGGAGGAGTTCCGCCAAACCCTATTAAAAGAAAGAGCAGAAAAGCTTGAGAATTGGAAGATGAAGGAAATGCTTAGGGAAGAGAAGAAAAAACAGAAGAATGAACTGCTACGACGTCAAAGTTCCCTATGGATTGATGAACCAGAACTGGAAAAGAAGATGCTTGAAGCCATTGTTGATACCAGACCCCTTTGA

Protein Analysis

185

Amino Acids

22.26

Weight (kDa)

9.92

Isoelectric Point (pI)

61.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014620)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06580
fragaria_vesca FvH4_4g20200 FvH4_4g20200
malus_domestica MD13G1150000.v1.1 MD16G1150400.v1.1
prunus_persica Prupe.1G114000_v2.0.a1
pyrus_communis pycom13g12920 pycom16g12880
rosa_chinensis RchiOBHm_Chr4g0425841
rosa_laevigata RLG00000007336
rosa_multiflora Rmu_sc0007063.1_g000006 Rmu_sc0009636.1_g000002
rosa_roxburghii Rroxscaffold_5G00367820
rosa_rugosa Rorug04G0206700
rosa_samantha Rh4CG282200 Rh4DG266100
rosa_wichuraiana Rw4G022770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 481
Acc36I ACCTGC 1 cut(s) 187
AccBSI CCGCTC 1 cut(s) 39
AccII CGCG 3 cut(s) 134, 170, 191
AciI CCGC 6 cut(s) 28, 37, 94, 211, 330, 376
AclWI GGATC 1 cut(s) 202
AcuI CTGAAG 1 cut(s) 278
AcyI GRCGYC 2 cut(s) 5, 478
AfaI GTAC 1 cut(s) 221
AfiI CCNNNNNNNGG 1 cut(s) 151
AgsI TTSAA 1 cut(s) 530
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 6 cut(s) 111, 126, 166, 238, 256, 409
AluI AGCT 6 cut(s) 111, 126, 166, 238, 256, 409
Alw21I GWGCWC 1 cut(s) 113
Alw26I GTCTC 1 cut(s) 177
AlwI GGATC 1 cut(s) 202
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 5 cut(s) 159, 270, 321, 327, 342
ApaI GGGCCC 1 cut(s) 346
AspLEI GCGC 5 cut(s) 136, 157, 172, 309, 354
AspS9I GGNCC 4 cut(s) 271, 337, 342, 343
AsuC2I CCSGG 2 cut(s) 13, 341
AsuHPI GGTGA 1 cut(s) 83
AvaI CYCGRG 1 cut(s) 248
AvaII GGWCC 1 cut(s) 337
AxyI CCTNAGG 1 cut(s) 287
BaeGI GKGCMC 1 cut(s) 346
BanII GRGCYC 2 cut(s) 113, 346
BbsI GAAGAC 1 cut(s) 9
Bbv12I GWGCWC 1 cut(s) 113
BciT130I CCWGG 1 cut(s) 356
BcnI CCSGG 2 cut(s) 13, 341
BcoDI GTCTC 1 cut(s) 177
BfoI RGCGCY 1 cut(s) 355
BfuAI ACCTGC 1 cut(s) 187
BisI GCNGC 1 cut(s) 330
BlsI GCNGC 1 cut(s) 331
Bme1390I CCNGG 3 cut(s) 13, 341, 356
Bme18I GGWCC 1 cut(s) 337
BmeT110I CYCGRG 1 cut(s) 248
BmgT120I GGNCC 4 cut(s) 271, 337, 342, 343
BmiI GGNNCC 1 cut(s) 344
BmrFI CCNGG 3 cut(s) 13, 341, 356
BmsI GCATC 2 cut(s) 181, 513
BpiI GAAGAC 1 cut(s) 9
BplI GAGNNNNNCTC 2 cut(s) 95, 127
Bpu10I CCTNAGC 2 cut(s) 333, 437
BpuEI CTTGAG 2 cut(s) 302, 431
BpuMI CCSGG 2 cut(s) 13, 341
BsaHI GRCGYC 2 cut(s) 5, 478
BsaJI CCNNGG 3 cut(s) 147, 324, 363
Bsc4I CCNNNNNNNGG 1 cut(s) 151
Bse1I ACTGG 1 cut(s) 516
Bse21I CCTNAGG 1 cut(s) 287
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 3 cut(s) 147, 324, 363
BseLI CCNNNNNNNGG 1 cut(s) 151
BseMII CTCAG 1 cut(s) 301
BseNI ACTGG 1 cut(s) 516
BseRI GAGGAG 3 cut(s) 317, 380, 383
BseSI GKGCMC 1 cut(s) 346
BseYI CCCAGC 1 cut(s) 126
Bsh1236I CGCG 3 cut(s) 134, 170, 191
BshFI GGCC 5 cut(s) 161, 272, 323, 329, 344
BsiHKAI GWGCWC 1 cut(s) 113
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 3 cut(s) 13, 178, 340
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 1 cut(s) 151
BsmAI GTCTC 1 cut(s) 177
BsmFI GGGAC 1 cut(s) 143
BsnI GGCC 5 cut(s) 161, 272, 323, 329, 344
BsoBI CYCGRG 1 cut(s) 248
Bsp120I GGGCCC 1 cut(s) 342
Bsp1286I GDGCHC 2 cut(s) 113, 346
Bsp143I GATC 1 cut(s) 207
BspACI CCGC 6 cut(s) 28, 37, 94, 211, 330, 376
BspANI GGCC 5 cut(s) 161, 272, 323, 329, 344
BspCNI CTCAG 1 cut(s) 300
BspFNI CGCG 3 cut(s) 134, 170, 191
BspLI GGNNCC 1 cut(s) 344
BspMI ACCTGC 1 cut(s) 187
BspPI GGATC 1 cut(s) 202
BsrBI CCGCTC 1 cut(s) 39
BsrI ACTGG 1 cut(s) 516
BssECI CCNNGG 3 cut(s) 147, 324, 363
BssMI GATC 1 cut(s) 207
BssNI GRCGYC 2 cut(s) 5, 478
BssT1I CCWWGG 1 cut(s) 147
Bst2UI CCWGG 1 cut(s) 356
Bst6I CTCTTC 1 cut(s) 438
BstACI GRCGYC 2 cut(s) 5, 478
BstC8I GCNNGC 2 cut(s) 159, 168
BstDEI CTNAG 3 cut(s) 287, 333, 437
BstFNI CGCG 3 cut(s) 134, 170, 191
BstH2I RGCGCY 1 cut(s) 355
BstHHI GCGC 5 cut(s) 136, 157, 172, 309, 354
BstKTI GATC 1 cut(s) 210
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 1 cut(s) 207
BstMWI GCNNNNNNNGC 8 cut(s) 36, 163, 167, 178, 244, 253, 313, 329
BstNI CCWGG 1 cut(s) 356
BstSCI CCNGG 3 cut(s) 11, 339, 354
BstSLI GKGCMC 1 cut(s) 346
BstUI CGCG 3 cut(s) 134, 170, 191
BstV2I GAAGAC 1 cut(s) 9
BstX2I RGATCY 1 cut(s) 207
BstYI RGATCY 1 cut(s) 207
Bsu36I CCTNAGG 1 cut(s) 287
BsuRI GGCC 5 cut(s) 161, 272, 323, 329, 344
BveI ACCTGC 1 cut(s) 187
Cac8I GCNNGC 2 cut(s) 159, 168
CfoI GCGC 5 cut(s) 136, 157, 172, 309, 354
Cfr13I GGNCC 4 cut(s) 271, 337, 342, 343
CseI GACGC 1 cut(s) 140
Csp6I GTAC 1 cut(s) 220
CviQI GTAC 1 cut(s) 220
DdeI CTNAG 3 cut(s) 287, 333, 437
DpnI GATC 1 cut(s) 209
DpnII GATC 1 cut(s) 207
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
EciI GGCGGA 1 cut(s) 365
Ecl136II GAGCTC 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 147
Eco24I GRGCYC 2 cut(s) 113, 346
Eco47I GGWCC 1 cut(s) 337
Eco53kI GAGCTC 1 cut(s) 111
Eco57I CTGAAG 1 cut(s) 278
Eco81I CCTNAGG 1 cut(s) 287
Eco88I CYCGRG 1 cut(s) 248
EcoICRI GAGCTC 1 cut(s) 111
EcoRII CCWGG 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 147
EcoT38I GRGCYC 2 cut(s) 113, 346
ErhI CCWWGG 1 cut(s) 147
FaiI YATR 1 cut(s) 493
FaqI GGGAC 1 cut(s) 143
Fnu4HI GCNGC 1 cut(s) 330
FriOI GRGCYC 2 cut(s) 113, 346
Fsp4HI GCNGC 1 cut(s) 330
GlaI GCGC 5 cut(s) 135, 156, 171, 308, 353
GluI GCNGC 1 cut(s) 330
GsaI CCCAGC 1 cut(s) 130
HaeII RGCGCY 1 cut(s) 355
HaeIII GGCC 5 cut(s) 161, 272, 323, 329, 344
HapII CCGG 3 cut(s) 13, 178, 340
HgaI GACGC 1 cut(s) 140
HhaI GCGC 5 cut(s) 136, 157, 172, 309, 354
Hin1I GRCGYC 2 cut(s) 5, 478
Hin6I GCGC 5 cut(s) 134, 155, 170, 307, 352
HinP1I GCGC 5 cut(s) 134, 155, 170, 307, 352
HindIII AAGCTT 1 cut(s) 407
HpaII CCGG 3 cut(s) 13, 178, 340
HphI GGTGA 1 cut(s) 83
Hpy188I TCNGA 3 cut(s) 122, 260, 297
Hpy188III TCNNGA 1 cut(s) 250
Hpy99I CGWCG 2 cut(s) 10, 480
HpyAV CCTTC 3 cut(s) 52, 124, 421
HpyCH4IV ACGT 1 cut(s) 478
HpyF10VI GCNNNNNNNGC 8 cut(s) 36, 163, 167, 178, 244, 253, 313, 329
HpyF3I CTNAG 3 cut(s) 287, 333, 437
HpySE526I ACGT 1 cut(s) 478
Hsp92I GRCGYC 2 cut(s) 5, 478
HspAI GCGC 5 cut(s) 134, 155, 170, 307, 352
Kzo9I GATC 1 cut(s) 207
LmnI GCTCC 4 cut(s) 44, 116, 261, 304
LweI GCATC 2 cut(s) 181, 513
MaeII ACGT 1 cut(s) 478
MalI GATC 1 cut(s) 209
MbiI CCGCTC 1 cut(s) 39
MboI GATC 1 cut(s) 207
MboII GAAGA 6 cut(s) 9, 433, 455, 460, 472, 532
MflI RGATCY 1 cut(s) 207
MhlI GDGCHC 2 cut(s) 113, 346
MluCI AATT 1 cut(s) 415
MseI TTAA 1 cut(s) 389
MspI CCGG 3 cut(s) 13, 178, 340
MspR9I CCNGG 3 cut(s) 13, 341, 356
MvaI CCWGG 1 cut(s) 356
MvnI CGCG 3 cut(s) 134, 170, 191
MwoI GCNNNNNNNGC 8 cut(s) 36, 163, 167, 178, 244, 253, 313, 329
NciI CCSGG 2 cut(s) 13, 341
NdeII GATC 1 cut(s) 207
NlaIV GGNNCC 1 cut(s) 344
NmeAIII GCCGAG 4 cut(s) 132, 162, 349, 388
PaeR7I CTCGAG 1 cut(s) 248
PkrI GCNGC 1 cut(s) 331
Psp124BI GAGCTC 1 cut(s) 113
Psp6I CCWGG 1 cut(s) 354
PspFI CCCAGC 1 cut(s) 126
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 344
PspOMI GGGCCC 1 cut(s) 342
PspPI GGNCC 4 cut(s) 271, 337, 342, 343
PsuI RGATCY 1 cut(s) 207
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
SacI GAGCTC 1 cut(s) 113
SaqAI TTAA 1 cut(s) 389
SatI GCNGC 1 cut(s) 330
Sau3AI GATC 1 cut(s) 207
Sau96I GGNCC 4 cut(s) 271, 337, 342, 343
ScrFI CCNGG 3 cut(s) 13, 341, 356
SduI GDGCHC 2 cut(s) 113, 346
SfaNI GCATC 2 cut(s) 181, 513
Sfr274I CTCGAG 1 cut(s) 248
SinI GGWCC 1 cut(s) 337
SlaI CTCGAG 1 cut(s) 248
SmlI CTYRAG 3 cut(s) 248, 317, 410
SmoI CTYRAG 3 cut(s) 248, 317, 410
Sse9I AATT 1 cut(s) 415
SsiI CCGC 6 cut(s) 28, 37, 94, 211, 330, 376
SstI GAGCTC 1 cut(s) 113
StyD4I CCNGG 3 cut(s) 11, 339, 354
StyI CCWWGG 1 cut(s) 147
TaiI ACGT 1 cut(s) 481
TaqI TCGA 2 cut(s) 234, 249
TasI AATT 1 cut(s) 415
TatI WGTACW 1 cut(s) 219
TauI GCSGC 1 cut(s) 332
Tru1I TTAA 1 cut(s) 389
Tru9I TTAA 1 cut(s) 389
TspDTI ATGAA 4 cut(s) 106, 440, 480, 516
VpaK11BI GGWCC 1 cut(s) 337
XhoI CTCGAG 1 cut(s) 248
ZraI GACGTC 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.