Rh4DG266100

gal1,galk

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014620
Physical Location & Seq
Forward (+)
48541560 .. 48545914
4355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG266100.1

Sequence Viewer

Length: 1182 bp
ATGGCTCAAATGATGAGGCACGAAGAACTCCCGATCCCAATCCACTCCGCTCTCGACCAAGTCTACGGCGATTCCTCGCAACTCGAGGAGGCCCAGCTCCGCTTCCACCGCCTCAAGACCAAGTTCCACGAGGTCTTCGGGCACCTCCCTCTACTCTGCGCTCGCTCTCCAGGGAGAGTGAACTTGATCGGAGAGCATATAGACTATGAGGGATACTCGGTGTTGCCGATGGCGATACGGCAGGATACTATTGTGGCGATAAGGAGGAGCGAAGGGGAGAAGGTTCTGAGAATTGCGAATGTTAACGATAAGTATGAAATGTGTACTTATCCTGCTGATCCTAATCAGGAAATTGATTTGAAGAATCACAAATGGGGCCATTATTTCATTTGTGGGTACAAAGGTTTCTATGAATTTGCCAGGTCCCGAGGAATAGATCTGGGTGAACCAGTGGGACTCGATGTTCTTATTGATGGAACAGTTCCTACAGGGTCTGGATTATCAAGCTCTGCAGCATTAGTTTGTGCTTCTACCATTGCTATTATGGCTGCTTTCGAGGAGAATTTTCCCAAGAAAGAAATTGCTCAACTTACATGTGATTGTGAGCGACACATTGGTACACAATCTGGTGGAATGGATCAGGCAATCTCTGTAATGGCTAAGCCTGGTTTTGCAGAACTAATTGATTTCAACCCTATTCGTGCTACTGATGTGCAACTTCCAGCTGGTGGAACTTTTGTTGTAGCCCATTCCTTGGCAGACTCTCAGAAAGCAGTAACTGCTGCTACCAATTACAACAACAGAGTTGTTGAATGTCGTCTGGCTTCAATTCTACTTGCTATAAAGTTGGGAATGAAGCCACAAGATGCAATTTCAAATGTCAAAACTCTTTCTGATGTTGAAGGGTTGTGCATGTCATATGCTAGTACCCATGGGTCTTCAGATCCTAACCTGGCTGTAAAGGAATTTTTGAAGGAAGAACCTTATACAGTTGATGAAATTGAGAAAATCACTGGGGAAAATCTAGAATCTGTCTTTCGAAATTCTGCATCTTCTCTGGATGTGTTGAAGGCTGCAAAACACTTCAAGTTATGTCAGAGAGCCTCCCATGTGTACTCTGAAGCCAAGCGGGTACATGCGTTCAAGGACACTGTATCATCAGATTTAAGGTATATTTATTAG

Protein Analysis

393

Amino Acids

43.44

Weight (kDa)

5.88

Isoelectric Point (pI)

40.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GalKase_gal_bdg PF10509 41 - 89 3.1e-22 Galactokinase galactose-binding signature
GHMP_kinases_N PF00288 144 - 220 1e-16 GHMP kinases N terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014620)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06580
fragaria_vesca FvH4_4g20200 FvH4_4g20200
malus_domestica MD13G1150000.v1.1 MD16G1150400.v1.1
prunus_persica Prupe.1G114000_v2.0.a1
pyrus_communis pycom13g12920 pycom16g12880
rosa_chinensis RchiOBHm_Chr4g0425841
rosa_laevigata RLG00000007336
rosa_multiflora Rmu_sc0007063.1_g000006 Rmu_sc0009636.1_g000002
rosa_roxburghii Rroxscaffold_5G00367820
rosa_rugosa Rorug04G0206700
rosa_samantha Rh4CG282200 Rh4DG266100
rosa_wichuraiana Rw4G022770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 141
AccB7I CCANNNNNTGG 2 cut(s) 728, 754
AccBSI CCGCTC 1 cut(s) 50
AccI GTMKAC 1 cut(s) 63
AciI CCGC 4 cut(s) 48, 100, 109, 1129
AclWI GGATC 4 cut(s) 28, 332, 645, 938
AcsI RAATTY 4 cut(s) 413, 562, 965, 1042
AcuI CTGAAG 2 cut(s) 924, 1140
AfaI GTAC 6 cut(s) 325, 398, 619, 928, 1115, 1134
AfiI CCNNNNNNNGG 2 cut(s) 728, 754
AflIII ACRYGT 1 cut(s) 593
AjnI CCWGG 4 cut(s) 169, 419, 664, 951
AloI GAACNNNNNNTCC 4 cut(s) 18, 50, 447, 479
AluBI AGCT 3 cut(s) 97, 507, 725
AluI AGCT 3 cut(s) 97, 507, 725
AlwI GGATC 4 cut(s) 28, 332, 645, 938
AlwNI CAGNNNCTG 2 cut(s) 494, 779
Ama87I CYCGRG 2 cut(s) 83, 426
AoxI GGCC 2 cut(s) 90, 376
ApeKI GCWGC 4 cut(s) 512, 548, 782, 1073
ApoI RAATTY 4 cut(s) 413, 562, 965, 1042
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 3 cut(s) 91, 376, 423
AsuHPI GGTGA 1 cut(s) 455
AsuII TTCGAA 1 cut(s) 1039
AvaI CYCGRG 2 cut(s) 83, 426
AvaII GGWCC 1 cut(s) 423
BaeGI GKGCMC 1 cut(s) 144
BaeI ACNNNNGTAYC 6 cut(s) 237, 270, 388, 421, 1124, 1157
BanI GGYRCC 1 cut(s) 141
BauI CACGAG 1 cut(s) 128
BbsI GAAGAC 2 cut(s) 127, 930
BbvI GCAGC 4 cut(s) 524, 535, 769, 1060
BccI CCATC 2 cut(s) 223, 467
BceAI ACGGC 2 cut(s) 82, 254
BciT130I CCWGG 4 cut(s) 171, 421, 666, 953
BciVI GTATCC 2 cut(s) 206, 238
BfaI CTAG 2 cut(s) 924, 1025
BfmI CTRYAG 2 cut(s) 486, 510
BfuI GTATCC 2 cut(s) 206, 238
BglII AGATCT 1 cut(s) 436
BisI GCNGC 4 cut(s) 513, 549, 783, 1074
BlpI GCTNAGC 1 cut(s) 660
BlsI GCNGC 4 cut(s) 514, 550, 784, 1075
Bme1390I CCNGG 4 cut(s) 171, 421, 666, 953
Bme18I GGWCC 1 cut(s) 423
BmeT110I CYCGRG 2 cut(s) 83, 426
BmgT120I GGNCC 3 cut(s) 91, 376, 423
BmiI GGNNCC 3 cut(s) 143, 377, 425
BmrFI CCNGG 4 cut(s) 171, 421, 666, 953
BmrI ACTGGG 1 cut(s) 1023
BmsI GCATC 2 cut(s) 856, 1058
BmuI ACTGGG 1 cut(s) 1023
BpiI GAAGAC 2 cut(s) 127, 930
BplI GAGNNNNNCTC 2 cut(s) 200, 232
BpmI CTGGAG 1 cut(s) 153
Bpu1102I GCTNAGC 1 cut(s) 660
Bpu14I TTCGAA 1 cut(s) 1039
BpuEI CTTGAG 1 cut(s) 98
BsaBI GATNNNNATC 2 cut(s) 38, 342
BsaJI CCNNGG 4 cut(s) 170, 427, 753, 931
Bsc4I CCNNNNNNNGG 2 cut(s) 728, 754
Bse1I ACTGG 2 cut(s) 449, 1018
Bse3DI GCAATG 1 cut(s) 534
Bse8I GATNNNNATC 2 cut(s) 38, 342
BseBI CCWGG 4 cut(s) 171, 421, 666, 953
BseDI CCNNGG 4 cut(s) 170, 427, 753, 931
BseGI GGATG 1 cut(s) 1066
BseJI GATNNNNATC 2 cut(s) 38, 342
BseLI CCNNNNNNNGG 2 cut(s) 728, 754
BseMI GCAATG 1 cut(s) 534
BseMII CTCAG 2 cut(s) 278, 779
BseNI ACTGG 2 cut(s) 449, 1018
BseRI GAGGAG 3 cut(s) 101, 280, 572
BseSI GKGCMC 1 cut(s) 144
BseXI GCAGC 4 cut(s) 524, 535, 769, 1060
BseYI CCCAGC 1 cut(s) 93
BshFI GGCC 2 cut(s) 92, 378
BshNI GGYRCC 1 cut(s) 141
BsiHKCI CYCGRG 2 cut(s) 83, 426
BslFI GGGAC 2 cut(s) 409, 468
BslI CCNNNNNNNGG 2 cut(s) 728, 754
BsmFI GGGAC 2 cut(s) 409, 468
BsnI GGCC 2 cut(s) 92, 378
BsoBI CYCGRG 2 cut(s) 83, 426
Bsp119I TTCGAA 1 cut(s) 1039
Bsp1286I GDGCHC 1 cut(s) 144
Bsp143I GATC 6 cut(s) 33, 186, 337, 436, 637, 943
Bsp1720I GCTNAGC 1 cut(s) 660
Bsp19I CCATGG 1 cut(s) 931
BspACI CCGC 4 cut(s) 48, 100, 109, 1129
BspANI GGCC 2 cut(s) 92, 378
BspCNI CTCAG 2 cut(s) 279, 778
BspLI GGNNCC 3 cut(s) 143, 377, 425
BspMAI CTGCAG 1 cut(s) 514
BspPI GGATC 4 cut(s) 28, 332, 645, 938
BspT104I TTCGAA 1 cut(s) 1039
BspT107I GGYRCC 1 cut(s) 141
BsrBI CCGCTC 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 534
BsrI ACTGG 2 cut(s) 449, 1018
BssECI CCNNGG 4 cut(s) 170, 427, 753, 931
BssMI GATC 6 cut(s) 33, 186, 337, 436, 637, 943
BssSI CACGAG 1 cut(s) 128
BssT1I CCWWGG 2 cut(s) 753, 931
Bst2BI CACGAG 1 cut(s) 128
Bst2UI CCWGG 4 cut(s) 171, 421, 666, 953
Bst4CI ACNGT 3 cut(s) 481, 991, 1153
BstAPI GCANNNNNTGC 1 cut(s) 779
BstBI TTCGAA 1 cut(s) 1039
BstC8I GCNNGC 1 cut(s) 163
BstDEI CTNAG 3 cut(s) 287, 660, 765
BstDSI CCRYGG 1 cut(s) 931
BstF5I GGATG 1 cut(s) 1066
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 6 cut(s) 36, 189, 340, 439, 640, 946
BstMBI GATC 6 cut(s) 33, 186, 337, 436, 637, 943
BstMWI GCNNNNNNNGC 3 cut(s) 108, 545, 779
BstNI CCWGG 4 cut(s) 171, 421, 666, 953
BstNSI RCATGY 3 cut(s) 597, 916, 1139
BstSCI CCNGG 4 cut(s) 169, 419, 664, 951
BstSFI CTRYAG 2 cut(s) 486, 510
BstSLI GKGCMC 1 cut(s) 144
BstV1I GCAGC 4 cut(s) 524, 535, 769, 1060
BstV2I GAAGAC 2 cut(s) 127, 930
BstX2I RGATCY 2 cut(s) 436, 943
BstYI RGATCY 2 cut(s) 436, 943
BsuI GTATCC 2 cut(s) 206, 238
BsuRI GGCC 2 cut(s) 92, 378
BtgI CCRYGG 1 cut(s) 931
BtsCI GGATG 1 cut(s) 1066
BtsIMutI CAGTG 3 cut(s) 456, 1011, 1149
Cac8I GCNNGC 1 cut(s) 163
CaiI CAGNNNCTG 2 cut(s) 494, 779
CfoI GCGC 1 cut(s) 161
Cfr13I GGNCC 3 cut(s) 91, 376, 423
Csp6I GTAC 6 cut(s) 324, 397, 618, 927, 1114, 1133
CviAII CATG 5 cut(s) 594, 913, 932, 1109, 1136
CviQI GTAC 6 cut(s) 324, 397, 618, 927, 1114, 1133
DdeI CTNAG 3 cut(s) 287, 660, 765
DpnI GATC 6 cut(s) 35, 188, 339, 438, 639, 945
DpnII GATC 6 cut(s) 33, 186, 337, 436, 637, 943
Eco130I CCWWGG 2 cut(s) 753, 931
Eco47I GGWCC 1 cut(s) 423
Eco57I CTGAAG 2 cut(s) 924, 1140
Eco88I CYCGRG 2 cut(s) 83, 426
EcoO109I RGGNCCY 1 cut(s) 423
EcoRII CCWGG 4 cut(s) 169, 419, 664, 951
EcoT14I CCWWGG 2 cut(s) 753, 931
ErhI CCWWGG 2 cut(s) 753, 931
FaeI CATG 5 cut(s) 597, 916, 935, 1112, 1139
FaqI GGGAC 2 cut(s) 409, 468
FatI CATG 5 cut(s) 593, 912, 931, 1108, 1135
FauI CCCGC 1 cut(s) 1122
FauNDI CATATG 1 cut(s) 919
FblI GTMKAC 1 cut(s) 63
Fnu4HI GCNGC 4 cut(s) 513, 549, 783, 1074
FokI GGATG 1 cut(s) 1073
Fsp4HI GCNGC 4 cut(s) 513, 549, 783, 1074
FspBI CTAG 2 cut(s) 924, 1025
GlaI GCGC 1 cut(s) 160
GluI GCNGC 4 cut(s) 513, 549, 783, 1074
GsaI CCCAGC 1 cut(s) 97
GsuI CTGGAG 1 cut(s) 153
HaeIII GGCC 2 cut(s) 92, 378
HhaI GCGC 1 cut(s) 161
Hin1II CATG 5 cut(s) 597, 916, 935, 1112, 1139
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HinfI GANTC 5 cut(s) 71, 364, 456, 761, 1028
HpaI GTTAAC 1 cut(s) 304
HphI GGTGA 1 cut(s) 455
Hpy166II GTNNAC 7 cut(s) 64, 181, 304, 324, 446, 620, 1114
Hpy188I TCNGA 8 cut(s) 191, 288, 768, 895, 943, 1098, 1120, 1162
Hpy188III TCNNGA 8 cut(s) 31, 53, 115, 347, 426, 495, 1025, 1058
Hpy8I GTNNAC 7 cut(s) 64, 181, 304, 324, 446, 620, 1114
HpyAV CCTTC 5 cut(s) 266, 274, 896, 967, 1063
HpyCH4III ACNGT 3 cut(s) 481, 991, 1153
HpyCH4V TGCA 7 cut(s) 512, 674, 715, 869, 912, 1049, 1076
HpyF10VI GCNNNNNNNGC 3 cut(s) 108, 545, 779
HpyF3I CTNAG 3 cut(s) 287, 660, 765
Hsp92II CATG 5 cut(s) 597, 916, 935, 1112, 1139
HspAI GCGC 1 cut(s) 159
KspAI GTTAAC 1 cut(s) 304
Kzo9I GATC 6 cut(s) 33, 186, 337, 436, 637, 943
LmnI GCTCC 2 cut(s) 102, 267
Lsp1109I GCAGC 4 cut(s) 524, 535, 769, 1060
LweI GCATC 2 cut(s) 856, 1058
MaeI CTAG 2 cut(s) 924, 1025
MaeIII GTNAC 1 cut(s) 775
MalI GATC 6 cut(s) 35, 188, 339, 438, 639, 945
MbiI CCGCTC 1 cut(s) 50
MboI GATC 6 cut(s) 33, 186, 337, 436, 637, 943
MboII GAAGA 6 cut(s) 35, 127, 373, 930, 989, 1044
MflI RGATCY 2 cut(s) 436, 943
MhlI GDGCHC 1 cut(s) 144
MlyI GAGTC 2 cut(s) 450, 755
MseI TTAA 2 cut(s) 303, 1166
MspA1I CMGCKG 1 cut(s) 725
MspR9I CCNGG 4 cut(s) 171, 421, 666, 953
MvaI CCWGG 4 cut(s) 171, 421, 666, 953
MwoI GCNNNNNNNGC 3 cut(s) 108, 545, 779
NcoI CCATGG 1 cut(s) 931
NdeI CATATG 1 cut(s) 919
NdeII GATC 6 cut(s) 33, 186, 337, 436, 637, 943
NlaIII CATG 5 cut(s) 597, 916, 935, 1112, 1139
NlaIV GGNNCC 3 cut(s) 143, 377, 425
NspI RCATGY 3 cut(s) 597, 916, 1139
NspV TTCGAA 1 cut(s) 1039
PaeR7I CTCGAG 1 cut(s) 83
PciI ACATGT 1 cut(s) 593
PcsI WCGNNNNNNNCGW 1 cut(s) 224
PfeI GAWTC 3 cut(s) 71, 364, 1028
PflFI GACNNNGTC 1 cut(s) 59
PflMI CCANNNNNTGG 2 cut(s) 728, 754
PkrI GCNGC 4 cut(s) 514, 550, 784, 1075
PleI GAGTC 2 cut(s) 450, 755
PpsI GAGTC 2 cut(s) 450, 755
PpuMI RGGWCCY 1 cut(s) 423
PscI ACATGT 1 cut(s) 593
Psp5II RGGWCCY 1 cut(s) 423
Psp6I CCWGG 4 cut(s) 169, 419, 664, 951
PspFI CCCAGC 1 cut(s) 93
PspGI CCWGG 4 cut(s) 169, 419, 664, 951
PspN4I GGNNCC 3 cut(s) 143, 377, 425
PspPI GGNCC 3 cut(s) 91, 376, 423
PspPPI RGGWCCY 1 cut(s) 423
PspXI VCTCGAGB 1 cut(s) 83
PsrI GAACNNNNNNTAC 2 cut(s) 469, 501
PstI CTGCAG 1 cut(s) 514
PstNI CAGNNNCTG 2 cut(s) 494, 779
PsuI RGATCY 2 cut(s) 436, 943
PsyI GACNNNGTC 1 cut(s) 59
PvuII CAGCTG 1 cut(s) 725
RsaI GTAC 6 cut(s) 325, 398, 619, 928, 1115, 1134
RsaNI GTAC 6 cut(s) 324, 397, 618, 927, 1114, 1133
SaqAI TTAA 2 cut(s) 303, 1166
SatI GCNGC 4 cut(s) 513, 549, 783, 1074
Sau3AI GATC 6 cut(s) 33, 186, 337, 436, 637, 943
Sau96I GGNCC 3 cut(s) 91, 376, 423
SchI GAGTC 2 cut(s) 450, 755
ScrFI CCNGG 4 cut(s) 171, 421, 666, 953
SduI GDGCHC 1 cut(s) 144
SfaNI GCATC 2 cut(s) 856, 1058
SfcI CTRYAG 2 cut(s) 486, 510
Sfr274I CTCGAG 1 cut(s) 83
SfuI TTCGAA 1 cut(s) 1039
SinI GGWCC 1 cut(s) 423
SlaI CTCGAG 1 cut(s) 83
SmlI CTYRAG 2 cut(s) 83, 113
SmoI CTYRAG 2 cut(s) 83, 113
SsiI CCGC 4 cut(s) 48, 100, 109, 1129
SspMI CTAG 2 cut(s) 924, 1025
StyD4I CCNGG 4 cut(s) 169, 419, 664, 951
StyI CCWWGG 2 cut(s) 753, 931
TaaI ACNGT 3 cut(s) 481, 991, 1153
TaqI TCGA 5 cut(s) 54, 84, 459, 555, 1039
TatI WGTACW 2 cut(s) 323, 1113
TfiI GAWTC 3 cut(s) 71, 364, 1028
Tru1I TTAA 2 cut(s) 303, 1166
Tru9I TTAA 2 cut(s) 303, 1166
TscAI CASTG 3 cut(s) 456, 1018, 1156
TseI GCWGC 4 cut(s) 512, 548, 782, 1073
TspDTI ATGAA 5 cut(s) 330, 376, 426, 869, 1011
TspRI CASTG 3 cut(s) 456, 1018, 1156
Tth111I GACNNNGTC 1 cut(s) 59
Van91I CCANNNNNTGG 2 cut(s) 728, 754
VpaK11BI GGWCC 1 cut(s) 423
XapI RAATTY 4 cut(s) 413, 562, 965, 1042
XbaI TCTAGA 1 cut(s) 1024
XceI RCATGY 3 cut(s) 597, 916, 1139
XcmI CCANNNNNNNNNTGG 1 cut(s) 541
XhoI CTCGAG 1 cut(s) 83
XmiI GTMKAC 1 cut(s) 63
XspI CTAG 2 cut(s) 924, 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.