FvH4_4g20380

Uncharacterised protein family UPF0052

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004211
Physical Location & Seq
Forward (+)
23670130 .. 23675523
5394 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g20380.t4

Sequence Viewer

Length: 1158 bp
ATGAATCTCACCACTCGCGTCGCGCACGTGCTTCCCGTCTCCGACGACGGCGGCAGCACCGCCGAGATCGTCCGCGTGCTCGGTGGTCCGGCCGTCGGAGATATCCGGTCCAGGTGCTTGAGATTGGCCGATAAAAGCACCTCCGAGGCCGTCGCCGTAAGGCAATTGCTTGGTCATCGGTTACCGGTTGATGCATCCCAAGCCAAATCCGAATGGTATGAGATTGTGGAAGGAGAGCACTCTTTGTGGACTGGAGTATCAAAGCCCTATAGGGAAACCATTCGAGCTTTTCTTGTTTATTTTCAGAATCAGATTCTCCGTCGAGCTGATGAATCGTTTTGCTTCAGTAATGGCAGCATTGGGAATTTCTTCTTTGCAGGAGCACGCGTATTTTTCCGGTCTTTGGATGCTGCAATATTTTTATTCTCTCGAGTGTCGGATATTCCATCAGAAAGTCTTGTTCTTCCAGTGATTTCTACTAATGACAGGCTTACATTAGGATGTGAATTATCGGATGGAACTATAATACGAGGGCAAAATGAAATTTCTCATCCAACCAAGGGATACACGGCACTTGTCAATAAGGGAAGCTCTTCAGCTCCCGCACTCTCTTCAAGGATAAAGAGGGTGTTCTACATGTCAAGTGAGGGCCAAAACTTGCTTCATGAGGTCTTCCCTGCTCCAAACCCAACTGTGATGGATCAGCTACGTAATGTAGGTTGCATTGTTTATGCCATGGGTTCTCTCTTTACTTCTGTCTGCCCATCACTGGTATTAATTGGCATCGGGGAGATTATATCTTCAAGGTCCTGCCCCAAGGTACTTTTGTTAAACGGTACCCATGATCGAGAGACAAGTGGCTTCTCAGCTTCTTGCTTTGTAACTGCTATTACTGATGCACTAAATCGAACATATGGAGGCCCTCATAACTGTCTCAAAAACCTTCCCAGTCACTATATCAATACCCTATTGGTGCCCAAAGATGGTGAGGTACCTGTTAATGTTCAATGCTTAGCTGACCAAGGAATTTTTGAAGTGATTGTTGTTGACTCTGTACATGATCCAAAAGTAGGTGTGGTGTTTGACCCCAAGTCGTTAATACAAGCCCTTGATGATTTGATAGGCAGACACATGCCTACAAGAGTTAAGGTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0052

Protein Analysis

386

Amino Acids

41.89

Weight (kDa)

6.79

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CofD PF01933 4 - 328 1.3e-16 2-phospho-L-lactate transferase CofD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 836, 991
AccB1I GGYRCC 3 cut(s) 836, 973, 991
AccII CGCG 4 cut(s) 18, 23, 75, 387
AciI CCGC 4 cut(s) 51, 60, 73, 603
AclWI GGATC 2 cut(s) 708, 1055
AcoI YGGCCR 2 cut(s) 90, 126
AcsI RAATTY 3 cut(s) 364, 543, 1026
AcuI CTGAAG 2 cut(s) 328, 579
AcvI CACGTG 1 cut(s) 28
AfaI GTAC 4 cut(s) 822, 838, 993, 1056
AfiI CCNNNNNNNGG 6 cut(s) 95, 403, 560, 769, 983, 1070
AflIII ACRYGT 2 cut(s) 385, 636
AgeI ACCGGT 1 cut(s) 184
AgsI TTSAA 4 cut(s) 615, 804, 1007, 1034
AhdI GACNNNNNGTC 1 cut(s) 1090
AjnI CCWGG 1 cut(s) 110
AjuI GAANNNNNNNTTGG 2 cut(s) 645, 677
AluBI AGCT 7 cut(s) 287, 326, 591, 599, 706, 869, 1016
AluI AGCT 7 cut(s) 287, 326, 591, 599, 706, 869, 1016
Alw21I GWGCWC 3 cut(s) 81, 240, 385
Alw26I GTCTC 3 cut(s) 43, 845, 938
AlwI GGATC 2 cut(s) 708, 1055
Ama87I CYCGRG 1 cut(s) 429
AoxI GGCC 5 cut(s) 90, 126, 147, 649, 919
ApeKI GCWGC 3 cut(s) 54, 354, 410
ApoI RAATTY 3 cut(s) 364, 543, 1026
AseI ATTAAT 1 cut(s) 776
AsiGI ACCGGT 1 cut(s) 184
Asp700I GAANNNNTTC 3 cut(s) 279, 368, 592
Asp718I GGTACC 2 cut(s) 836, 991
AspLEI GCGC 1 cut(s) 25
AspS9I GGNCC 5 cut(s) 86, 108, 649, 807, 920
AsuHPI GGTGA 1 cut(s) 998
AvaI CYCGRG 1 cut(s) 429
AvaII GGWCC 3 cut(s) 86, 108, 807
BaeGI GKGCMC 1 cut(s) 978
BaeI ACNNNNGTAYC 4 cut(s) 240, 273, 812, 845
BanI GGYRCC 3 cut(s) 836, 973, 991
BbrPI CACGTG 1 cut(s) 28
BbsI GAAGAC 1 cut(s) 664
Bbv12I GWGCWC 3 cut(s) 81, 240, 385
BbvI GCAGC 3 cut(s) 66, 366, 397
BccI CCATC 5 cut(s) 454, 509, 691, 772, 977
BceAI ACGGC 5 cut(s) 64, 77, 134, 140, 585
BciT130I CCWGG 1 cut(s) 112
BciVI GTATCC 1 cut(s) 557
BcoDI GTCTC 3 cut(s) 43, 845, 938
BfmI CTRYAG 1 cut(s) 268
BfuI GTATCC 1 cut(s) 557
BisI GCNGC 4 cut(s) 52, 55, 355, 411
BlpI GCTNAGC 1 cut(s) 1012
BlsI GCNGC 4 cut(s) 53, 56, 356, 412
Bme1390I CCNGG 1 cut(s) 112
Bme18I GGWCC 3 cut(s) 86, 108, 807
BmeRI GACNNNNNGTC 1 cut(s) 1090
BmeT110I CYCGRG 1 cut(s) 429
BmgT120I GGNCC 5 cut(s) 86, 108, 649, 807, 920
BmiI GGNNCC 3 cut(s) 838, 975, 993
BmrFI CCNGG 1 cut(s) 112
BmrI ACTGGG 1 cut(s) 942
BmsI GCATC 5 cut(s) 181, 203, 397, 792, 886
BmuI ACTGGG 1 cut(s) 942
BpiI GAAGAC 1 cut(s) 664
BpmI CTGGAG 1 cut(s) 273
Bpu1102I GCTNAGC 1 cut(s) 1012
BpuEI CTTGAG 1 cut(s) 139
BsaAI YACGTR 2 cut(s) 28, 710
BsaJI CCNNGG 5 cut(s) 144, 558, 735, 816, 1021
BsaWI WCCGGW 3 cut(s) 105, 184, 396
BsaXI ACNNNNNCTCC 2 cut(s) 372, 402
Bsc4I CCNNNNNNNGG 6 cut(s) 95, 403, 560, 769, 983, 1070
Bse118I RCCGGY 1 cut(s) 184
Bse1I ACTGG 4 cut(s) 256, 467, 774, 948
BseBI CCWGG 1 cut(s) 112
BseDI CCNNGG 5 cut(s) 144, 558, 735, 816, 1021
BseGI GGATG 5 cut(s) 194, 412, 506, 520, 550
BseLI CCNNNNNNNGG 6 cut(s) 95, 403, 560, 769, 983, 1070
BseMII CTCAG 1 cut(s) 879
BseNI ACTGG 4 cut(s) 256, 467, 774, 948
BseSI GKGCMC 1 cut(s) 978
BseX3I CGGCCG 1 cut(s) 90
BseXI GCAGC 3 cut(s) 66, 366, 397
Bsh1236I CGCG 4 cut(s) 18, 23, 75, 387
Bsh1285I CGRYCG 1 cut(s) 93
BshFI GGCC 5 cut(s) 92, 128, 149, 651, 921
BshNI GGYRCC 3 cut(s) 836, 973, 991
BshTI ACCGGT 1 cut(s) 184
BsiEI CGRYCG 1 cut(s) 93
BsiHKAI GWGCWC 3 cut(s) 81, 240, 385
BsiHKCI CYCGRG 1 cut(s) 429
BsiSI CCGG 4 cut(s) 89, 106, 185, 397
BslI CCNNNNNNNGG 6 cut(s) 95, 403, 560, 769, 983, 1070
BsmAI GTCTC 3 cut(s) 43, 845, 938
BsmBI CGTCTC 1 cut(s) 43
BsnI GGCC 5 cut(s) 92, 128, 149, 651, 921
BsoBI CYCGRG 1 cut(s) 429
Bsp1286I GDGCHC 4 cut(s) 81, 240, 385, 978
Bsp1407I TGTACA 1 cut(s) 1054
Bsp143I GATC 4 cut(s) 66, 700, 844, 1060
Bsp1720I GCTNAGC 1 cut(s) 1012
Bsp19I CCATGG 1 cut(s) 735
BspACI CCGC 4 cut(s) 51, 60, 73, 603
BspANI GGCC 5 cut(s) 92, 128, 149, 651, 921
BspCNI CTCAG 1 cut(s) 878
BspFNI CGCG 4 cut(s) 18, 23, 75, 387
BspHI TCATGA 1 cut(s) 664
BspLI GGNNCC 3 cut(s) 838, 975, 993
BspPI GGATC 2 cut(s) 708, 1055
BspQI GCTCTTC 1 cut(s) 598
BspT107I GGYRCC 3 cut(s) 836, 973, 991
BsrFI RCCGGY 1 cut(s) 184
BsrGI TGTACA 1 cut(s) 1054
BsrI ACTGG 4 cut(s) 256, 467, 774, 948
BssAI RCCGGY 1 cut(s) 184
BssECI CCNNGG 5 cut(s) 144, 558, 735, 816, 1021
BssMI GATC 4 cut(s) 66, 700, 844, 1060
BssT1I CCWWGG 4 cut(s) 558, 735, 816, 1021
Bst2UI CCWGG 1 cut(s) 112
Bst4CI ACNGT 3 cut(s) 694, 836, 932
Bst6I CTCTTC 2 cut(s) 598, 616
BstAUI TGTACA 1 cut(s) 1054
BstBAI YACGTR 2 cut(s) 28, 710
BstC8I GCNNGC 2 cut(s) 77, 385
BstDEI CTNAG 2 cut(s) 865, 1012
BstDSI CCRYGG 1 cut(s) 735
BstEII GGTNACC 1 cut(s) 180
BstF5I GGATG 5 cut(s) 194, 412, 506, 520, 550
BstFNI CGCG 4 cut(s) 18, 23, 75, 387
BstHHI GCGC 1 cut(s) 25
BstKTI GATC 4 cut(s) 69, 703, 847, 1063
BstMAI GTCTC 3 cut(s) 43, 845, 938
BstMBI GATC 4 cut(s) 66, 700, 844, 1060
BstMCI CGRYCG 1 cut(s) 93
BstMWI GCNNNNNNNGC 1 cut(s) 200
BstNI CCWGG 1 cut(s) 112
BstNSI RCATGY 2 cut(s) 640, 1135
BstPI GGTNACC 1 cut(s) 180
BstSCI CCNGG 1 cut(s) 110
BstSFI CTRYAG 1 cut(s) 268
BstSLI GKGCMC 1 cut(s) 978
BstSNI TACGTA 1 cut(s) 710
BstUI CGCG 4 cut(s) 18, 23, 75, 387
BstV1I GCAGC 3 cut(s) 66, 366, 397
BstV2I GAAGAC 1 cut(s) 664
BstZI CGGCCG 1 cut(s) 90
BsuI GTATCC 1 cut(s) 557
BsuRI GGCC 5 cut(s) 92, 128, 149, 651, 921
BtgI CCRYGG 1 cut(s) 735
BtsCI GGATG 5 cut(s) 194, 412, 506, 520, 550
BtsIMutI CAGTG 2 cut(s) 474, 767
Cac8I GCNNGC 2 cut(s) 77, 385
CciI TCATGA 1 cut(s) 664
CfoI GCGC 1 cut(s) 25
Cfr10I RCCGGY 1 cut(s) 184
Cfr13I GGNCC 5 cut(s) 86, 108, 649, 807, 920
CseI GACGC 1 cut(s) 7
Csp6I GTAC 4 cut(s) 821, 837, 992, 1055
CspAI ACCGGT 1 cut(s) 184
CviAII CATG 6 cut(s) 637, 665, 736, 842, 1058, 1132
CviQI GTAC 4 cut(s) 821, 837, 992, 1055
DdeI CTNAG 2 cut(s) 865, 1012
DpnI GATC 4 cut(s) 68, 702, 846, 1062
DpnII GATC 4 cut(s) 66, 700, 844, 1060
DriI GACNNNNNGTC 1 cut(s) 1090
EaeI YGGCCR 2 cut(s) 90, 126
EagI CGGCCG 1 cut(s) 90
Eam1104I CTCTTC 2 cut(s) 598, 616
Eam1105I GACNNNNNGTC 1 cut(s) 1090
EarI CTCTTC 2 cut(s) 598, 616
EclXI CGGCCG 1 cut(s) 90
Eco105I TACGTA 1 cut(s) 710
Eco130I CCWWGG 4 cut(s) 558, 735, 816, 1021
Eco32I GATATC 1 cut(s) 103
Eco47I GGWCC 3 cut(s) 86, 108, 807
Eco52I CGGCCG 1 cut(s) 90
Eco57I CTGAAG 2 cut(s) 328, 579
Eco72I CACGTG 1 cut(s) 28
Eco88I CYCGRG 1 cut(s) 429
Eco91I GGTNACC 1 cut(s) 180
EcoO109I RGGNCCY 2 cut(s) 807, 920
EcoO65I GGTNACC 1 cut(s) 180
EcoRII CCWGG 1 cut(s) 110
EcoRV GATATC 1 cut(s) 103
EcoT14I CCWWGG 4 cut(s) 558, 735, 816, 1021
EcoT22I ATGCAT 1 cut(s) 196
ErhI CCWWGG 4 cut(s) 558, 735, 816, 1021
Esp3I CGTCTC 1 cut(s) 43
FaeI CATG 6 cut(s) 640, 668, 739, 845, 1061, 1135
FatI CATG 6 cut(s) 636, 664, 735, 841, 1057, 1131
FauI CCCGC 1 cut(s) 610
FauNDI CATATG 1 cut(s) 913
Fnu4HI GCNGC 4 cut(s) 52, 55, 355, 411
FokI GGATG 5 cut(s) 181, 419, 513, 527, 537
Fsp4HI GCNGC 4 cut(s) 52, 55, 355, 411
GlaI GCGC 1 cut(s) 24
GluI GCNGC 4 cut(s) 52, 55, 355, 411
GsuI CTGGAG 1 cut(s) 273
HaeIII GGCC 5 cut(s) 92, 128, 149, 651, 921
HapII CCGG 4 cut(s) 89, 106, 185, 397
HgaI GACGC 1 cut(s) 7
HhaI GCGC 1 cut(s) 25
Hin1II CATG 6 cut(s) 640, 668, 739, 845, 1061, 1135
Hin6I GCGC 1 cut(s) 23
HinP1I GCGC 1 cut(s) 23
HincII GTYRAC 1 cut(s) 1048
HindII GTYRAC 1 cut(s) 1048
HinfI GANTC 5 cut(s) 4, 307, 313, 332, 1049
HpaII CCGG 4 cut(s) 89, 106, 185, 397
HphI GGTGA 1 cut(s) 998
Hpy166II GTNNAC 2 cut(s) 249, 1048
Hpy188I TCNGA 9 cut(s) 43, 98, 145, 211, 306, 312, 439, 451, 514
Hpy188III TCNNGA 3 cut(s) 429, 665, 848
Hpy8I GTNNAC 2 cut(s) 249, 1048
Hpy99I CGWCG 6 cut(s) 23, 47, 50, 98, 155, 324
HpyAV CCTTC 2 cut(s) 224, 953
HpyCH4III ACNGT 3 cut(s) 694, 836, 932
HpyCH4IV ACGT 2 cut(s) 27, 709
HpyCH4V TGCA 5 cut(s) 194, 377, 413, 723, 899
HpyF10VI GCNNNNNNNGC 1 cut(s) 200
HpyF3I CTNAG 2 cut(s) 865, 1012
HpySE526I ACGT 2 cut(s) 27, 709
Hsp92II CATG 6 cut(s) 640, 668, 739, 845, 1061, 1135
HspAI GCGC 1 cut(s) 23
KpnI GGTACC 2 cut(s) 840, 995
Kzo9I GATC 4 cut(s) 66, 700, 844, 1060
LguI GCTCTTC 1 cut(s) 598
LmnI GCTCC 3 cut(s) 380, 604, 685
Lsp1109I GCAGC 3 cut(s) 66, 366, 397
LweI GCATC 5 cut(s) 181, 203, 397, 792, 886
MaeII ACGT 2 cut(s) 27, 709
MaeIII GTNAC 3 cut(s) 180, 880, 950
MalI GATC 4 cut(s) 68, 702, 846, 1062
MboI GATC 4 cut(s) 66, 700, 844, 1060
MboII GAAGA 6 cut(s) 361, 455, 585, 603, 664, 792
MfeI CAATTG 1 cut(s) 164
MhlI GDGCHC 4 cut(s) 81, 240, 385, 978
MluCI AATT 6 cut(s) 164, 364, 506, 543, 777, 1026
MluI ACGCGT 1 cut(s) 385
MlyI GAGTC 1 cut(s) 1043
MmeI TCCRAC 4 cut(s) 66, 76, 417, 578
MnlI CCTC 9 cut(s) 139, 151, 524, 618, 640, 661, 911, 933, 982
Mph1103I ATGCAT 1 cut(s) 196
MroXI GAANNNNTTC 3 cut(s) 279, 368, 592
MseI TTAA 5 cut(s) 776, 830, 999, 1097, 1146
MslI CAYNNNNRTG 1 cut(s) 499
MspI CCGG 4 cut(s) 89, 106, 185, 397
MspR9I CCNGG 1 cut(s) 112
MunI CAATTG 1 cut(s) 164
MvaI CCWGG 1 cut(s) 112
MvnI CGCG 4 cut(s) 18, 23, 75, 387
MwoI GCNNNNNNNGC 1 cut(s) 200
NcoI CCATGG 1 cut(s) 735
NdeI CATATG 1 cut(s) 913
NdeII GATC 4 cut(s) 66, 700, 844, 1060
NlaIII CATG 6 cut(s) 640, 668, 739, 845, 1061, 1135
NlaIV GGNNCC 3 cut(s) 838, 975, 993
NmeAIII GCCGAG 1 cut(s) 88
NmuCI GTSAC 1 cut(s) 950
NsiI ATGCAT 1 cut(s) 196
NspI RCATGY 2 cut(s) 640, 1135
PaeR7I CTCGAG 1 cut(s) 429
PagI TCATGA 1 cut(s) 664
PciI ACATGT 1 cut(s) 636
PciSI GCTCTTC 1 cut(s) 598
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PdmI GAANNNNTTC 3 cut(s) 279, 368, 592
PfeI GAWTC 4 cut(s) 4, 307, 313, 332
PinAI ACCGGT 1 cut(s) 184
PkrI GCNGC 4 cut(s) 53, 56, 356, 412
PleI GAGTC 1 cut(s) 1043
PmaCI CACGTG 1 cut(s) 28
PmlI CACGTG 1 cut(s) 28
PpsI GAGTC 1 cut(s) 1043
Ppu21I YACGTR 2 cut(s) 28, 710
PpuMI RGGWCCY 1 cut(s) 807
PscI ACATGT 1 cut(s) 636
PshBI ATTAAT 1 cut(s) 776
Psp5II RGGWCCY 1 cut(s) 807
Psp6I CCWGG 1 cut(s) 110
PspCI CACGTG 1 cut(s) 28
PspEI GGTNACC 1 cut(s) 180
PspGI CCWGG 1 cut(s) 110
PspN4I GGNNCC 3 cut(s) 838, 975, 993
PspPI GGNCC 5 cut(s) 86, 108, 649, 807, 920
PspPPI RGGWCCY 1 cut(s) 807
RsaI GTAC 4 cut(s) 822, 838, 993, 1056
RsaNI GTAC 4 cut(s) 821, 837, 992, 1055
RseI CAYNNNNRTG 1 cut(s) 499
SapI GCTCTTC 1 cut(s) 598
SaqAI TTAA 5 cut(s) 776, 830, 999, 1097, 1146
SatI GCNGC 4 cut(s) 52, 55, 355, 411
Sau3AI GATC 4 cut(s) 66, 700, 844, 1060
Sau96I GGNCC 5 cut(s) 86, 108, 649, 807, 920
SchI GAGTC 1 cut(s) 1043
ScrFI CCNGG 1 cut(s) 112
SduI GDGCHC 4 cut(s) 81, 240, 385, 978
SfaNI GCATC 5 cut(s) 181, 203, 397, 792, 886
SfcI CTRYAG 1 cut(s) 268
Sfr274I CTCGAG 1 cut(s) 429
SinI GGWCC 3 cut(s) 86, 108, 807
SlaI CTCGAG 1 cut(s) 429
SmiMI CAYNNNNRTG 1 cut(s) 499
SmlI CTYRAG 2 cut(s) 118, 429
SmoI CTYRAG 2 cut(s) 118, 429
SnaBI TACGTA 1 cut(s) 710
Sse9I AATT 6 cut(s) 164, 364, 506, 543, 777, 1026
SsiI CCGC 4 cut(s) 51, 60, 73, 603
SspI AATATT 1 cut(s) 417
StyD4I CCNGG 1 cut(s) 110
StyI CCWWGG 4 cut(s) 558, 735, 816, 1021
TaaI ACNGT 3 cut(s) 694, 836, 932
TaiI ACGT 2 cut(s) 30, 712
TaqI TCGA 5 cut(s) 283, 322, 430, 847, 907
TasI AATT 6 cut(s) 164, 364, 506, 543, 777, 1026
TatI WGTACW 1 cut(s) 1054
TauI GCSGC 1 cut(s) 54
TfiI GAWTC 4 cut(s) 4, 307, 313, 332
Tru1I TTAA 5 cut(s) 776, 830, 999, 1097, 1146
Tru9I TTAA 5 cut(s) 776, 830, 999, 1097, 1146
TscAI CASTG 2 cut(s) 474, 774
TseFI GTSAC 1 cut(s) 950
TseI GCWGC 3 cut(s) 54, 354, 410
Tsp45I GTSAC 1 cut(s) 950
TspDTI ATGAA 4 cut(s) 17, 345, 555, 653
TspGWI ACGGA 1 cut(s) 308
TspRI CASTG 2 cut(s) 474, 774
VpaK11BI GGWCC 3 cut(s) 86, 108, 807
VspI ATTAAT 1 cut(s) 776
XapI RAATTY 3 cut(s) 364, 543, 1026
XceI RCATGY 2 cut(s) 640, 1135
XhoI CTCGAG 1 cut(s) 429
XmnI GAANNNNTTC 3 cut(s) 279, 368, 592
Zsp2I ATGCAT 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.