Rroxscaffold_1G00065780

Uncharacterised protein family UPF0052

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004211
Physical Location & Seq
Forward (+)
87376529 .. 87381210
4682 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00065780.1

Sequence Viewer

Length: 1287 bp
ATGTCGACTCTCCGCTCCCTAAACCCTAACCCTTCCTGCTCCTCCATTCCCAATCCCAACCCCGCCGCCCAGCCTTCCCTCCTCGTCTTCTCCGGCGGCACCGCCTTCAACGGCGTCGTCGAGGACCTCAAGACTTTCACCGCTCGCGTCGCCCACGTCCTTCCCGTTTCCGACGACGGAGGCAGCACCGCCGAGATCGTCCGCGTCCTCGGTGGTCCGGCCGTCGGAGATATACGGTCCAGGTGCTTGAGATTGGCCGATAAAAGCACCTCCGAGGCCGTCGCCGTCCGGCAATTGCTCGGTCATCGTTTACCATTCGATGCACGCCAAGCCAAGTCCGAATGGTATGATATTGTGGAAGGAGAACACTCTTTATGGACAGGAGTTTCAAAGCCCTACAGGGAAACCATTCGAGCTTTTCTGGTTTATTTTCAGAATCAGATTCTCCGTCGATCTGATGAATCGTTTTGCTTCAGTAATGGCAGCATTGGGAATTTCTTCTTTGCAGGAGCACGCGTATTTTTTCGGTCTTTGGATGCTGCAATATTTTTGTTTTCTCGAGTGTCAGATATCCCATCAGAAAGTCTGGTTCTTCCAGTGATTTCTACTAATGACAGGCTTACATTAGGATGTGAATTATCGGATGGAACTATAATACGAGGGCAGAATGAAATTTCTCATCCAACCAAGGGATATACGGCACTTGTCAATAAGGGAAGCTCTTCAGCTCCTGCACTCTCTTCGAGGATAAAGAGGGTATTCTACATGTCAAGTGAGGGCCAAAACTTGCTTCATGAGGTCTTCCCTGCTCCAAACCCAGCTGTGATGGATCAGCTACGTAATGTGGATTGCATTGTTTATGCCATGGGTTCTCTCTTTACTTCCGTTTGCCCATCACTGGTATTAATTGGCATCGGGGAGATTATATCTTCAAGGTCCTGCCCCAAGGTACTTTTGTTAAATGGTACCCATGATCGAGAGACAAGTGGCTTCTCAGCTTCTTGCTTTGTAACTGCTATTACAGATGCACTAAATCGAACATATGGAGCCCCTCATAACTGTCTCAAAAACCTTCCCAGTCATTATATCAATACCCTATTAGTGCCCAAAGATGGTGAGATACCTGTTAATGTTCAATGCTTAGCTGACCAAGGAATTTTTGAAGTGATTGTTGTTGACTCTGTACATGATCCAAAAGTAGGTGTGGTATTTGACCCCAAGTCGTTAATACAAGCCCTTGATGATTTGATAAGCAGACGCATGCCTACAAATGTTAAGGTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0052

Protein Analysis

428

Amino Acids

46.45

Weight (kDa)

6.75

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CofD PF01933 29 - 375 4.8e-39 2-phospho-L-lactate transferase CofD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 965
AccB1I GGYRCC 2 cut(s) 98, 965
AccBSI CCGCTC 2 cut(s) 15, 143
AccI GTMKAC 1 cut(s) 5
AccII CGCG 3 cut(s) 147, 204, 516
AciI CCGC 8 cut(s) 13, 63, 66, 96, 102, 141, 189, 202
AclWI GGATC 2 cut(s) 837, 1184
AcoI YGGCCR 2 cut(s) 219, 255
AcsI RAATTY 3 cut(s) 493, 672, 1155
AcuI CTGAAG 2 cut(s) 457, 708
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 3 cut(s) 951, 967, 1185
AfiI CCNNNNNNNGG 5 cut(s) 224, 689, 898, 1112, 1199
AflIII ACRYGT 2 cut(s) 514, 765
AgsI TTSAA 5 cut(s) 109, 390, 933, 1136, 1163
AhdI GACNNNNNGTC 1 cut(s) 1219
AjiI CACGTC 1 cut(s) 157
AjnI CCWGG 1 cut(s) 239
AjuI GAANNNNNNNTTGG 2 cut(s) 774, 806
AluBI AGCT 7 cut(s) 416, 720, 728, 821, 835, 998, 1145
AluI AGCT 7 cut(s) 416, 720, 728, 821, 835, 998, 1145
Alw21I GWGCWC 1 cut(s) 514
Alw26I GTCTC 2 cut(s) 974, 1067
AlwI GGATC 2 cut(s) 837, 1184
AlwNI CAGNNNCTG 1 cut(s) 731
Ama87I CYCGRG 1 cut(s) 558
AoxI GGCC 4 cut(s) 219, 255, 276, 778
ApeKI GCWGC 3 cut(s) 183, 483, 539
ApoI RAATTY 3 cut(s) 493, 672, 1155
AseI ATTAAT 1 cut(s) 905
Asp700I GAANNNNTTC 3 cut(s) 408, 497, 721
Asp718I GGTACC 1 cut(s) 965
AspS9I GGNCC 5 cut(s) 124, 215, 237, 778, 936
AsuHPI GGTGA 2 cut(s) 130, 1127
AvaI CYCGRG 1 cut(s) 558
AvaII GGWCC 4 cut(s) 124, 215, 237, 936
BaeGI GKGCMC 1 cut(s) 1107
BaeI ACNNNNGTAYC 2 cut(s) 941, 974
BanI GGYRCC 2 cut(s) 98, 965
BanII GRGCYC 1 cut(s) 1051
BbsI GAAGAC 2 cut(s) 79, 793
Bbv12I GWGCWC 1 cut(s) 514
BbvI GCAGC 3 cut(s) 195, 495, 526
BccI CCATC 5 cut(s) 583, 638, 820, 901, 1106
BceAI ACGGC 5 cut(s) 127, 206, 263, 269, 714
BcgI CGANNNNNNTGC 2 cut(s) 723, 757
BciT130I CCWGG 1 cut(s) 241
BcoDI GTCTC 2 cut(s) 974, 1067
BfmI CTRYAG 1 cut(s) 397
BisI GCNGC 5 cut(s) 66, 97, 184, 484, 540
BlpI GCTNAGC 1 cut(s) 1141
BlsI GCNGC 5 cut(s) 67, 98, 185, 485, 541
Bme1390I CCNGG 1 cut(s) 241
Bme18I GGWCC 4 cut(s) 124, 215, 237, 936
BmeRI GACNNNNNGTC 1 cut(s) 1219
BmeT110I CYCGRG 1 cut(s) 558
BmgBI CACGTC 1 cut(s) 157
BmgT120I GGNCC 5 cut(s) 124, 215, 237, 778, 936
BmiI GGNNCC 3 cut(s) 100, 967, 1048
BmrFI CCNGG 1 cut(s) 241
BmrI ACTGGG 1 cut(s) 1071
BmsI GCATC 4 cut(s) 310, 526, 921, 1015
BmuI ACTGGG 1 cut(s) 1071
BpiI GAAGAC 2 cut(s) 79, 793
Bpu1102I GCTNAGC 1 cut(s) 1141
BpuEI CTTGAG 2 cut(s) 113, 268
BsaAI YACGTR 1 cut(s) 839
BsaHI GRCGYC 1 cut(s) 114
BsaJI CCNNGG 6 cut(s) 208, 273, 687, 864, 945, 1150
BsaXI ACNNNNNCTCC 2 cut(s) 501, 531
Bsc4I CCNNNNNNNGG 5 cut(s) 224, 689, 898, 1112, 1199
Bse1I ACTGG 3 cut(s) 596, 903, 1077
BseBI CCWGG 1 cut(s) 241
BseDI CCNNGG 6 cut(s) 208, 273, 687, 864, 945, 1150
BseGI GGATG 4 cut(s) 541, 635, 649, 679
BseLI CCNNNNNNNGG 5 cut(s) 224, 689, 898, 1112, 1199
BseMII CTCAG 1 cut(s) 1008
BseNI ACTGG 3 cut(s) 596, 903, 1077
BseRI GAGGAG 2 cut(s) 31, 71
BseSI GKGCMC 1 cut(s) 1107
BseX3I CGGCCG 1 cut(s) 219
BseXI GCAGC 3 cut(s) 195, 495, 526
BseYI CCCAGC 2 cut(s) 69, 817
BsgI GTGCAG 1 cut(s) 717
Bsh1236I CGCG 3 cut(s) 147, 204, 516
Bsh1285I CGRYCG 1 cut(s) 222
BshFI GGCC 4 cut(s) 221, 257, 278, 780
BshNI GGYRCC 2 cut(s) 98, 965
BsiEI CGRYCG 1 cut(s) 222
BsiHKAI GWGCWC 1 cut(s) 514
BsiHKCI CYCGRG 1 cut(s) 558
BsiSI CCGG 3 cut(s) 93, 218, 289
BslI CCNNNNNNNGG 5 cut(s) 224, 689, 898, 1112, 1199
BsmAI GTCTC 2 cut(s) 974, 1067
BsnI GGCC 4 cut(s) 221, 257, 278, 780
BsoBI CYCGRG 1 cut(s) 558
Bsp1286I GDGCHC 3 cut(s) 514, 1051, 1107
Bsp1407I TGTACA 1 cut(s) 1183
Bsp143I GATC 5 cut(s) 195, 452, 829, 973, 1189
Bsp1720I GCTNAGC 1 cut(s) 1141
Bsp19I CCATGG 1 cut(s) 864
BspACI CCGC 8 cut(s) 13, 63, 66, 96, 102, 141, 189, 202
BspANI GGCC 4 cut(s) 221, 257, 278, 780
BspCNI CTCAG 1 cut(s) 1007
BspFNI CGCG 3 cut(s) 147, 204, 516
BspHI TCATGA 1 cut(s) 793
BspLI GGNNCC 3 cut(s) 100, 967, 1048
BspPI GGATC 2 cut(s) 837, 1184
BspQI GCTCTTC 1 cut(s) 727
BspT107I GGYRCC 2 cut(s) 98, 965
BsrBI CCGCTC 2 cut(s) 15, 143
BsrGI TGTACA 1 cut(s) 1183
BsrI ACTGG 3 cut(s) 596, 903, 1077
BssECI CCNNGG 6 cut(s) 208, 273, 687, 864, 945, 1150
BssMI GATC 5 cut(s) 195, 452, 829, 973, 1189
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 4 cut(s) 687, 864, 945, 1150
Bst2UI CCWGG 1 cut(s) 241
Bst4CI ACNGT 2 cut(s) 237, 1061
Bst6I CTCTTC 2 cut(s) 727, 745
BstACI GRCGYC 1 cut(s) 114
BstAUI TGTACA 1 cut(s) 1183
BstBAI YACGTR 1 cut(s) 839
BstC8I GCNNGC 4 cut(s) 145, 325, 514, 1262
BstDEI CTNAG 2 cut(s) 994, 1141
BstDSI CCRYGG 1 cut(s) 864
BstF5I GGATG 4 cut(s) 541, 635, 649, 679
BstFNI CGCG 3 cut(s) 147, 204, 516
BstKTI GATC 5 cut(s) 198, 455, 832, 976, 1192
BstMAI GTCTC 2 cut(s) 974, 1067
BstMBI GATC 5 cut(s) 195, 452, 829, 973, 1189
BstMCI CGRYCG 1 cut(s) 222
BstMWI GCNNNNNNNGC 2 cut(s) 149, 329
BstNI CCWGG 1 cut(s) 241
BstNSI RCATGY 2 cut(s) 769, 1264
BstSCI CCNGG 1 cut(s) 239
BstSFI CTRYAG 1 cut(s) 397
BstSLI GKGCMC 1 cut(s) 1107
BstSNI TACGTA 1 cut(s) 839
BstUI CGCG 3 cut(s) 147, 204, 516
BstV1I GCAGC 3 cut(s) 195, 495, 526
BstV2I GAAGAC 2 cut(s) 79, 793
BstZI CGGCCG 1 cut(s) 219
BsuRI GGCC 4 cut(s) 221, 257, 278, 780
BtgI CCRYGG 1 cut(s) 864
BtrI CACGTC 1 cut(s) 157
BtsCI GGATG 4 cut(s) 541, 635, 649, 679
BtsIMutI CAGTG 2 cut(s) 603, 896
Cac8I GCNNGC 4 cut(s) 145, 325, 514, 1262
CaiI CAGNNNCTG 1 cut(s) 731
CciI TCATGA 1 cut(s) 793
Cfr13I GGNCC 5 cut(s) 124, 215, 237, 778, 936
CseI GACGC 4 cut(s) 103, 136, 193, 1266
Csp6I GTAC 3 cut(s) 950, 966, 1184
CviAII CATG 6 cut(s) 766, 794, 865, 971, 1187, 1261
CviQI GTAC 3 cut(s) 950, 966, 1184
DdeI CTNAG 2 cut(s) 994, 1141
DpnI GATC 5 cut(s) 197, 454, 831, 975, 1191
DpnII GATC 5 cut(s) 195, 452, 829, 973, 1189
DriI GACNNNNNGTC 1 cut(s) 1219
EaeI YGGCCR 2 cut(s) 219, 255
EagI CGGCCG 1 cut(s) 219
Eam1104I CTCTTC 2 cut(s) 727, 745
Eam1105I GACNNNNNGTC 1 cut(s) 1219
EarI CTCTTC 2 cut(s) 727, 745
EclXI CGGCCG 1 cut(s) 219
Eco105I TACGTA 1 cut(s) 839
Eco130I CCWWGG 4 cut(s) 687, 864, 945, 1150
Eco24I GRGCYC 1 cut(s) 1051
Eco32I GATATC 1 cut(s) 571
Eco47I GGWCC 4 cut(s) 124, 215, 237, 936
Eco52I CGGCCG 1 cut(s) 219
Eco57I CTGAAG 2 cut(s) 457, 708
Eco88I CYCGRG 1 cut(s) 558
EcoO109I RGGNCCY 2 cut(s) 124, 936
EcoRII CCWGG 1 cut(s) 239
EcoRV GATATC 1 cut(s) 571
EcoT14I CCWWGG 4 cut(s) 687, 864, 945, 1150
EcoT38I GRGCYC 1 cut(s) 1051
ErhI CCWWGG 4 cut(s) 687, 864, 945, 1150
FaeI CATG 6 cut(s) 769, 797, 868, 974, 1190, 1264
FatI CATG 6 cut(s) 765, 793, 864, 970, 1186, 1260
FauI CCCGC 1 cut(s) 70
FauNDI CATATG 1 cut(s) 1042
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 5 cut(s) 66, 97, 184, 484, 540
FokI GGATG 4 cut(s) 548, 642, 656, 666
FriOI GRGCYC 1 cut(s) 1051
Fsp4HI GCNGC 5 cut(s) 66, 97, 184, 484, 540
GluI GCNGC 5 cut(s) 66, 97, 184, 484, 540
GsaI CCCAGC 2 cut(s) 73, 821
HaeIII GGCC 4 cut(s) 221, 257, 278, 780
HapII CCGG 3 cut(s) 93, 218, 289
HgaI GACGC 4 cut(s) 103, 136, 193, 1266
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 6 cut(s) 769, 797, 868, 974, 1190, 1264
HincII GTYRAC 2 cut(s) 6, 1177
HindII GTYRAC 2 cut(s) 6, 1177
HinfI GANTC 5 cut(s) 7, 436, 442, 461, 1178
HpaII CCGG 3 cut(s) 93, 218, 289
HphI GGTGA 2 cut(s) 130, 1127
Hpy166II GTNNAC 3 cut(s) 6, 311, 1177
Hpy188III TCNNGA 4 cut(s) 130, 558, 794, 977
Hpy8I GTNNAC 3 cut(s) 6, 311, 1177
Hpy99I CGWCG 8 cut(s) 119, 122, 152, 176, 179, 227, 284, 453
HpyAV CCTTC 6 cut(s) 42, 84, 115, 170, 353, 1082
HpyCH4III ACNGT 2 cut(s) 237, 1061
HpyCH4IV ACGT 2 cut(s) 156, 838
HpyCH4V TGCA 6 cut(s) 323, 506, 542, 734, 852, 1028
HpyF10VI GCNNNNNNNGC 2 cut(s) 149, 329
HpyF3I CTNAG 2 cut(s) 994, 1141
HpySE526I ACGT 2 cut(s) 156, 838
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 6 cut(s) 769, 797, 868, 974, 1190, 1264
KpnI GGTACC 1 cut(s) 969
Kzo9I GATC 5 cut(s) 195, 452, 829, 973, 1189
LguI GCTCTTC 1 cut(s) 727
LmnI GCTCC 6 cut(s) 20, 44, 509, 733, 814, 1046
Lsp1109I GCAGC 3 cut(s) 195, 495, 526
LweI GCATC 4 cut(s) 310, 526, 921, 1015
MaeII ACGT 2 cut(s) 156, 838
MaeIII GTNAC 1 cut(s) 1009
MalI GATC 5 cut(s) 197, 454, 831, 975, 1191
MbiI CCGCTC 2 cut(s) 15, 143
MboI GATC 5 cut(s) 195, 452, 829, 973, 1189
MboII GAAGA 7 cut(s) 79, 490, 584, 714, 732, 793, 921
MfeI CAATTG 1 cut(s) 293
MhlI GDGCHC 3 cut(s) 514, 1051, 1107
MluCI AATT 6 cut(s) 293, 493, 635, 672, 906, 1155
MluI ACGCGT 1 cut(s) 514
MlyI GAGTC 1 cut(s) 1172
MmeI TCCRAC 3 cut(s) 195, 205, 707
MroXI GAANNNNTTC 3 cut(s) 408, 497, 721
MseI TTAA 5 cut(s) 905, 959, 1128, 1226, 1275
MslI CAYNNNNRTG 1 cut(s) 628
MspA1I CMGCKG 1 cut(s) 821
MspI CCGG 3 cut(s) 93, 218, 289
MspR9I CCNGG 1 cut(s) 241
MunI CAATTG 1 cut(s) 293
MvaI CCWGG 1 cut(s) 241
MvnI CGCG 3 cut(s) 147, 204, 516
MwoI GCNNNNNNNGC 2 cut(s) 149, 329
NcoI CCATGG 1 cut(s) 864
NdeI CATATG 1 cut(s) 1042
NdeII GATC 5 cut(s) 195, 452, 829, 973, 1189
NlaIII CATG 6 cut(s) 769, 797, 868, 974, 1190, 1264
NlaIV GGNNCC 3 cut(s) 100, 967, 1048
NmeAIII GCCGAG 1 cut(s) 217
NspI RCATGY 2 cut(s) 769, 1264
PaeI GCATGC 1 cut(s) 1264
PaeR7I CTCGAG 1 cut(s) 558
PagI TCATGA 1 cut(s) 793
PciI ACATGT 1 cut(s) 765
PciSI GCTCTTC 1 cut(s) 727
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 162
PdmI GAANNNNTTC 3 cut(s) 408, 497, 721
PfeI GAWTC 3 cut(s) 436, 442, 461
PkrI GCNGC 5 cut(s) 67, 98, 185, 485, 541
PleI GAGTC 1 cut(s) 1172
PpsI GAGTC 1 cut(s) 1172
Ppu21I YACGTR 1 cut(s) 839
PpuMI RGGWCCY 2 cut(s) 124, 936
PscI ACATGT 1 cut(s) 765
PshBI ATTAAT 1 cut(s) 905
Psp5II RGGWCCY 2 cut(s) 124, 936
Psp6I CCWGG 1 cut(s) 239
PspFI CCCAGC 2 cut(s) 69, 817
PspGI CCWGG 1 cut(s) 239
PspN4I GGNNCC 3 cut(s) 100, 967, 1048
PspPI GGNCC 5 cut(s) 124, 215, 237, 778, 936
PspPPI RGGWCCY 2 cut(s) 124, 936
PstNI CAGNNNCTG 1 cut(s) 731
PvuII CAGCTG 1 cut(s) 821
RsaI GTAC 3 cut(s) 951, 967, 1185
RsaNI GTAC 3 cut(s) 950, 966, 1184
RseI CAYNNNNRTG 1 cut(s) 628
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 727
SaqAI TTAA 5 cut(s) 905, 959, 1128, 1226, 1275
SatI GCNGC 5 cut(s) 66, 97, 184, 484, 540
Sau3AI GATC 5 cut(s) 195, 452, 829, 973, 1189
Sau96I GGNCC 5 cut(s) 124, 215, 237, 778, 936
SchI GAGTC 1 cut(s) 1172
ScrFI CCNGG 1 cut(s) 241
SduI GDGCHC 3 cut(s) 514, 1051, 1107
SfaNI GCATC 4 cut(s) 310, 526, 921, 1015
SfcI CTRYAG 1 cut(s) 397
Sfr274I CTCGAG 1 cut(s) 558
SinI GGWCC 4 cut(s) 124, 215, 237, 936
SlaI CTCGAG 1 cut(s) 558
SmiMI CAYNNNNRTG 1 cut(s) 628
SmlI CTYRAG 3 cut(s) 128, 247, 558
SmoI CTYRAG 3 cut(s) 128, 247, 558
SnaBI TACGTA 1 cut(s) 839
SphI GCATGC 1 cut(s) 1264
Sse9I AATT 6 cut(s) 293, 493, 635, 672, 906, 1155
SsiI CCGC 8 cut(s) 13, 63, 66, 96, 102, 141, 189, 202
SspI AATATT 1 cut(s) 546
StyD4I CCNGG 1 cut(s) 239
StyI CCWWGG 4 cut(s) 687, 864, 945, 1150
TaaI ACNGT 2 cut(s) 237, 1061
TaiI ACGT 2 cut(s) 159, 841
TaqI TCGA 9 cut(s) 5, 120, 318, 412, 451, 559, 743, 976, 1036
TaqII GACCGA 2 cut(s) 290, 516
TasI AATT 6 cut(s) 293, 493, 635, 672, 906, 1155
TatI WGTACW 1 cut(s) 1183
TauI GCSGC 2 cut(s) 68, 99
TfiI GAWTC 3 cut(s) 436, 442, 461
Tru1I TTAA 5 cut(s) 905, 959, 1128, 1226, 1275
Tru9I TTAA 5 cut(s) 905, 959, 1128, 1226, 1275
TscAI CASTG 2 cut(s) 603, 903
TseI GCWGC 3 cut(s) 183, 483, 539
TspDTI ATGAA 3 cut(s) 474, 684, 782
TspGWI ACGGA 3 cut(s) 192, 437, 874
TspRI CASTG 2 cut(s) 603, 903
VpaK11BI GGWCC 4 cut(s) 124, 215, 237, 936
VspI ATTAAT 1 cut(s) 905
XapI RAATTY 3 cut(s) 493, 672, 1155
XceI RCATGY 2 cut(s) 769, 1264
XhoI CTCGAG 1 cut(s) 558
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 3 cut(s) 408, 497, 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.