Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0004734

Physical Location & Seq

Genomic Coordinates Reverse (-)

25140324 .. 25144235

Gene Structure 3912 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g22260.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2205 bp

ATGGGAGAGAAAGCAGTGAGGACGGAGGATGCGGCGGCCGGAGATAAGATGGGAGCAGAGATGCGCCGGATAGACTCCCTTAATATCGAGGCCGGAAAAGTTTCCACTTATGATGGGTTTCATCGAGCAAAGCATAGTTGGAAGACCACGTTGAGTTTAGCAATACAAAGCGTGGGGGTTGTGTATGGGGACATTGGGACTTCTCCACTCTATGTGTACTCGAGCACTTTCCCAAATGGTATTAAAGAAAAGGATGACATTCTAGGGGTGTTGTCTATCATCATATATACCATTTTCTTCGTGCCTATGCTGAAGTATGTCTTCATCGTCTTGCAGGCGAATGACAATGGGGAAGGTGGAACATTTGCAATGTATTCTTTGATCTGCCGGCATGCAAAAGTGAGCTTAATTCCTAATAACCAGCCAGAGGATACGGAGCTATCCAATTACAAACTGGACACCCCCTCGAGCGAGTTGAAACGTTCCCAAGCGATCAAGAAGAAGCTGGAGACAAGTAGACTTGCCCAGTATGTTCTTTTTGTAATCACCATTACCGGAACGTCCATGGTAATCGGAGATGGTGTTCTTACTCCATGCATTTCCGTTCTTTCAGCGGTGAGCGGATTGCAGCAGAGCGGGATCAAGTCACTAGACCAAAGTGCTATTGTGTGGATTTCAGTAGCAATCTTGATCCTTCTTTTTGTCGTTCAACAATTTGGGACTGATAAAGTGGGCTTCACATTTGCTCCTATAATCTTGTTGTGGTTTTTGTTCTTGACTGGGATTGGAATTTACAACTTGATCAAATACGATATTACTGTATTGAAAGCTTTCAATCCATGGTACATCGTGAAATATTTCCAAAGAAATGGAAAAGCTGCTTGGATTTCCCTTGGGGGAGCAGTTCTCTGCATCACCGGGACTGAAGCCATGTTTGCTGATCTGGGTCACTTCAGTGTGCGAGCAATCCAAGTCAGTTTTACTTTTTTCACATTTCCCACTATACTCTTCGCGTATCTTGGGCAATCGGCATACCTCACCAAGAACCCAGGAGATGTGGCCACCACTTTCTACGCCTCAATACCAAAACCTTTGTACTGGCCAACATTCGTTGTAGCCGTACTTGCTGCCATCATTGCCAGCCAAGCTTTGATTACCGGAACATTCTCAATCATTGCACAGTCCTTAGGCATGGGTTGCTTCCCTAGAGTCAAGATTGTCCACACCTCTGAAAAGAACCAGGGCCAAGTCTATATTCCTGAGATCAACTACATGCTTATGATAGCCTGCGTGCTCATCACCTTCTTTTTCAGAACCACTGAGCAAATTGGCAATGCTTATGGAATCGCGGTGGTCAGTGTTATGTGTATCACAACGTGTATGATCACCCTAATCATGTTAGTCATATGGAAGACCAGCATCTTTCTGATTGCCATCTTCTTCCTGGTGTTCATTTCAATCGAAGGGATTTACTTATCTTCGGTCCTGTTCAAATTCGTGGAAGGCGGTTACCTTCCTTTGTTCTTCGCTGCCGTTCTCATGGTGATTATGGCAACTTGGCATTACGTGCACAAACAAAGTTACATGTTTGAGGTCAAGAACAAGGTCTCTACCGAATACGTGAAGCAATTGGCCTTAAACCCCGACGTACATCGCATCCCTGGAATGGGGTTTTTGTATTCGGATCTCGTTCAAGGCGTTCCTCCCATATTTTCTCACTTTGTTTCCAACATACCTTCAGTACACTCGGTTGTGGTTATAGTGACCATCAAGCCGCTCCCGGTGAGCAAAGTTCTACCGGAGGAGAGGTTTGTTTTTCGACAGTTGGAACCGAAGGAATACAGAATGTTCCGTTGCGTGGCTCGGTATGGCTACAATGACTTGGTAGAGGGACCGGGAGAGTTCGAGCAGATGCTGGTGGACAATCTGAAGGAGTTCATTAGAAATAAACATGTGATGAGCGAAGGGAATTCGAAAAACAGTTATGAGGAGGAAATACAGTTCATACAAGAGGCAATGGACAAAGGGGTTGTGTATCTGATGGGAGAGGCACAAGTAGTGGCAGAAGAGAAGTCGAGTTGGTTCAAGAAAATTGTGATCAACTACTTCTACGATTTCTTGAGGAAAAACTCGAGGCAAAGTGGCAAAATCATGTCAATCCCACGAAGCAAGCTTCTTATGGTTGGAATGACCTATGACATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0008150 GO:0008324 GO:0009674 GO:0009987 GO:0010107 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015291 GO:0015293 GO:0015294 GO:0015318 GO:0015370 GO:0015672 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0035725 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

735

Amino Acids

82.28

Weight (kDa)

8.67

Isoelectric Point (pI)

34.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	53 - 539	7.4e-166	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	554 - 734	9.1e-27	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004734)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13420
fragaria_vesca	FvH4_4g22240 FvH4_4g22240 FvH4_4g22260
malus_domestica	MD13G1133100.v1.1 MD13G1133200.v1.1 MD16G1143900.v1.1
prunus_persica	Prupe.1G212100_v2.0.a1 Prupe.1G212200_v2.0.a1
pyrus_communis	pycom13g11630
rosa_chinensis	RchiOBHm_Chr4g0428481 RchiOBHm_Chr4g0428501
rosa_laevigata	RLG00000007145 RLG00000007146
rosa_multiflora	Rmu_sc0002072.1_g000037 Rmu_sc0004623.1_g000012 Rmu_sc0006844.1_g000008
rosa_roxburghii	Rroxscaffold_5G00370180 Rroxscaffold_5G00370210
rosa_rugosa	Rorug04G0225500 Rorug04G0225600
rosa_samantha	Rh4AG283100 Rh4AG283200 Rh4BG288800 Rh4BG288900 Rh4CG304400 Rh4CG304900 Rh4DG285900 Rh4DG286000
rosa_wichuraiana	Rw4G024580 Rw4G024600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	3 cut(s)	621, 636, 1777
AccI	GTMKAC	1 cut(s)	517
AccII	CGCG	2 cut(s)	1013, 1349
AciI	CCGC	8 cut(s)	32, 35, 614, 621, 636, 1349, 1506, 1775
AclI	AACGTT	1 cut(s)	481
AclWI	GGATC	3 cut(s)	647, 685, 1692
AcoI	YGGCCR	3 cut(s)	36, 1059, 1100
AcsI	RAATTY	3 cut(s)	789, 1493, 1969
AcuI	CTGAAG	5 cut(s)	332, 937, 945, 1722, 1949
AdeI	CACNNNGTG	1 cut(s)	1377
AfaI	GTAC	6 cut(s)	218, 845, 1097, 1122, 1650, 1743
AfiI	CCNNNNNNNGG	4 cut(s)	427, 1666, 1667, 1858
AflIII	ACRYGT	3 cut(s)	1376, 1584, 1951
AgsI	TTSAA	8 cut(s)	478, 710, 826, 835, 1458, 1492, 1694, 2086
AjnI	CCWGG	4 cut(s)	1048, 1239, 1443, 1660
AjuI	GAANNNNNNNTTGG	4 cut(s)	865, 897, 1239, 1271
AleI	CACNNNNGTG	1 cut(s)	954
AloI	GAACNNNNNNTCC	4 cut(s)	567, 599, 1985, 2017
AluBI	AGCT	7 cut(s)	405, 439, 505, 830, 878, 1148, 2173
AluI	AGCT	7 cut(s)	405, 439, 505, 830, 878, 1148, 2173
Alw21I	GWGCWC	3 cut(s)	227, 1296, 1572
Alw26I	GTCTC	2 cut(s)	503, 1612
Alw44I	GTGCAC	1 cut(s)	1568
AlwI	GGATC	3 cut(s)	647, 685, 1692
AlwNI	CAGNNNCTG	1 cut(s)	1915
Ama87I	CYCGRG	3 cut(s)	220, 466, 2131
AoxI	GGCC	6 cut(s)	36, 90, 1059, 1100, 1243, 1632
ApaLI	GTGCAC	1 cut(s)	1568
ApeKI	GCWGC	4 cut(s)	628, 878, 1127, 1529
ApoI	RAATTY	3 cut(s)	789, 1493, 1969
Asp700I	GAANNNNTTC	4 cut(s)	100, 830, 857, 1934
AspLEI	GCGC	1 cut(s)	66
AspS9I	GGNCC	3 cut(s)	1243, 1483, 1892
AsuC2I	CCSGG	3 cut(s)	919, 1781, 1896
AsuHPI	GGTGA	8 cut(s)	538, 628, 907, 1030, 1291, 1378, 1555, 1795
AsuII	TTCGAA	1 cut(s)	1973
AvaI	CYCGRG	3 cut(s)	220, 466, 2131
AvaII	GGWCC	2 cut(s)	1483, 1892
AxyI	CCTNAGG	1 cut(s)	1186
BaeGI	GKGCMC	1 cut(s)	1572
BalI	TGGCCA	2 cut(s)	1061, 1102
BbsI	GAAGAC	3 cut(s)	149, 313, 1418
Bbv12I	GWGCWC	3 cut(s)	227, 1296, 1572
BbvI	GCAGC	4 cut(s)	640, 865, 1114, 1516
BccI	CCATC	7 cut(s)	43, 107, 572, 1139, 1442, 1775, 2035
BceAI	ACGGC	2 cut(s)	1103, 1517
BciT130I	CCWGG	4 cut(s)	1050, 1241, 1445, 1662
BciVI	GTATCC	1 cut(s)	424
BclI	TGATCA	3 cut(s)	801, 1383, 2097
BcnI	CCSGG	3 cut(s)	919, 1781, 1896
BcoDI	GTCTC	2 cut(s)	503, 1612
BfaI	CTAG	3 cut(s)	263, 650, 1206
BfuI	GTATCC	1 cut(s)	424
BisI	GCNGC	7 cut(s)	33, 36, 629, 879, 1128, 1530, 1775
BlsI	GCNGC	7 cut(s)	34, 37, 630, 880, 1129, 1531, 1776
Bme1390I	CCNGG	7 cut(s)	919, 1050, 1241, 1445, 1662, 1781, 1896
Bme18I	GGWCC	2 cut(s)	1483, 1892
BmeT110I	CYCGRG	3 cut(s)	220, 466, 2131
BmgT120I	GGNCC	3 cut(s)	1243, 1483, 1892
BmiI	GGNNCC	2 cut(s)	1830, 1893
BmrFI	CCNGG	7 cut(s)	919, 1050, 1241, 1445, 1662, 1781, 1896
BmrI	ACTGGG	2 cut(s)	520, 789
BmsI	GCATC	6 cut(s)	19, 51, 921, 1428, 1665, 1902
BmuI	ACTGGG	2 cut(s)	520, 789
BpiI	GAAGAC	3 cut(s)	149, 313, 1418
BplI	GAGNNNNNCTC	2 cut(s)	891, 923
BpmI	CTGGAG	1 cut(s)	527
Bpu14I	TTCGAA	1 cut(s)	1973
BpuEI	CTTGAG	1 cut(s)	2140
BpuMI	CCSGG	3 cut(s)	919, 1781, 1896
BsaAI	YACGTR	2 cut(s)	1567, 1621
BsaBI	GATNNNNATC	1 cut(s)	1433
BsaI	GGTCTC	1 cut(s)	1612
BsaJI	CCNNGG	6 cut(s)	564, 839, 892, 1048, 1240, 1660
BsaWI	WCCGGW	3 cut(s)	554, 1157, 1798
BsaXI	ACNNNNNCTCC	6 cut(s)	567, 597, 730, 760, 1793, 1823
Bsc4I	CCNNNNNNNGG	4 cut(s)	427, 1666, 1667, 1858
Bse118I	RCCGGY	1 cut(s)	387
Bse1I	ACTGG	4 cut(s)	459, 526, 784, 1103
Bse21I	CCTNAGG	1 cut(s)	1186
Bse3DI	GCAATG	5 cut(s)	375, 1134, 1173, 1339, 2022
Bse8I	GATNNNNATC	1 cut(s)	1433
BseBI	CCWGG	4 cut(s)	1050, 1241, 1445, 1662
BseDI	CCNNGG	6 cut(s)	564, 839, 892, 1048, 1240, 1660
BseGI	GGATG	3 cut(s)	34, 259, 1656
BseJI	GATNNNNATC	1 cut(s)	1433
BseLI	CCNNNNNNNGG	4 cut(s)	427, 1666, 1667, 1858
BseMI	GCAATG	5 cut(s)	375, 1134, 1173, 1339, 2022
BseMII	CTCAG	2 cut(s)	1251, 1311
BseNI	ACTGG	4 cut(s)	459, 526, 784, 1103
BseRI	GAGGAG	2 cut(s)	1817, 2003
BseSI	GKGCMC	1 cut(s)	1572
BseX3I	CGGCCG	1 cut(s)	36
BseXI	GCAGC	4 cut(s)	640, 865, 1114, 1516
Bsh1236I	CGCG	2 cut(s)	1013, 1349
Bsh1285I	CGRYCG	1 cut(s)	39
BshFI	GGCC	6 cut(s)	38, 92, 1061, 1102, 1245, 1634
BsiEI	CGRYCG	1 cut(s)	39
BsiHKAI	GWGCWC	3 cut(s)	227, 1296, 1572
BsiHKCI	CYCGRG	3 cut(s)	220, 466, 2131
BslFI	GGGAC	5 cut(s)	203, 211, 733, 934, 1905
BslI	CCNNNNNNNGG	4 cut(s)	427, 1666, 1667, 1858
BsmAI	GTCTC	2 cut(s)	503, 1612
BsmFI	GGGAC	5 cut(s)	203, 211, 733, 934, 1905
BsnI	GGCC	6 cut(s)	38, 92, 1061, 1102, 1245, 1634
Bso31I	GGTCTC	1 cut(s)	1612
BsoBI	CYCGRG	3 cut(s)	220, 466, 2131
Bsp119I	TTCGAA	1 cut(s)	1973
Bsp1286I	GDGCHC	3 cut(s)	227, 1296, 1572
Bsp19I	CCATGG	2 cut(s)	564, 839
BspACI	CCGC	8 cut(s)	32, 35, 614, 621, 636, 1349, 1506, 1775
BspANI	GGCC	6 cut(s)	38, 92, 1061, 1102, 1245, 1634
BspCNI	CTCAG	2 cut(s)	1252, 1312
BspFNI	CGCG	2 cut(s)	1013, 1349
BspLI	GGNNCC	2 cut(s)	1830, 1893
BspPI	GGATC	3 cut(s)	647, 685, 1692
BspT104I	TTCGAA	1 cut(s)	1973
BspTNI	GGTCTC	1 cut(s)	1612
BsrBI	CCGCTC	3 cut(s)	621, 636, 1777
BsrDI	GCAATG	5 cut(s)	375, 1134, 1173, 1339, 2022
BsrFI	RCCGGY	1 cut(s)	387
BsrI	ACTGG	4 cut(s)	459, 526, 784, 1103
BssAI	RCCGGY	1 cut(s)	387
BssECI	CCNNGG	6 cut(s)	564, 839, 892, 1048, 1240, 1660
BssT1I	CCWWGG	3 cut(s)	564, 839, 892
Bst2UI	CCWGG	4 cut(s)	1050, 1241, 1445, 1662
Bst4CI	ACNGT	5 cut(s)	820, 1182, 1824, 1982, 2001
Bst6I	CTCTTC	2 cut(s)	1013, 2061
BstAPI	GCANNNNNTGC	2 cut(s)	1197, 1567
BstBAI	YACGTR	2 cut(s)	1567, 1621
BstBI	TTCGAA	1 cut(s)	1973
BstC8I	GCNNGC	8 cut(s)	336, 389, 393, 963, 1141, 1288, 1292, 2171
BstDEI	CTNAG	3 cut(s)	1186, 1260, 1320
BstDSI	CCRYGG	2 cut(s)	564, 839
BstEII	GGTNACC	1 cut(s)	1508
BstF5I	GGATG	3 cut(s)	34, 259, 1656
BstFNI	CGCG	2 cut(s)	1013, 1349
BstHHI	GCGC	1 cut(s)	66
BstMAI	GTCTC	2 cut(s)	503, 1612
BstMCI	CGRYCG	1 cut(s)	39
BstMWI	GCNNNNNNNGC	6 cut(s)	935, 1124, 1136, 1145, 1197, 1567
BstNI	CCWGG	4 cut(s)	1050, 1241, 1445, 1662
BstNSI	RCATGY	4 cut(s)	395, 1276, 1588, 1955
BstPI	GGTNACC	1 cut(s)	1508
BstSCI	CCNGG	7 cut(s)	917, 1048, 1239, 1443, 1660, 1779, 1894
BstSLI	GKGCMC	1 cut(s)	1572
BstUI	CGCG	2 cut(s)	1013, 1349
BstV1I	GCAGC	4 cut(s)	640, 865, 1114, 1516
BstV2I	GAAGAC	3 cut(s)	149, 313, 1418
BstX2I	RGATCY	1 cut(s)	1684
BstXI	CCANNNNNNTGG	1 cut(s)	869
BstYI	RGATCY	1 cut(s)	1684
BstZI	CGGCCG	1 cut(s)	36
Bsu36I	CCTNAGG	1 cut(s)	1186
BsuI	GTATCC	1 cut(s)	424
BsuRI	GGCC	6 cut(s)	38, 92, 1061, 1102, 1245, 1634
BtgI	CCRYGG	2 cut(s)	564, 839
BtgZI	GCGATG	1 cut(s)	1637
BtsCI	GGATG	3 cut(s)	34, 259, 1656
BtsI	GCAGTG	1 cut(s)	21
BtsIMutI	CAGTG	4 cut(s)	21, 961, 1317, 1363
Cac8I	GCNNGC	8 cut(s)	336, 389, 393, 963, 1141, 1288, 1292, 2171
CaiI	CAGNNNCTG	1 cut(s)	1915
CfoI	GCGC	1 cut(s)	66
Cfr10I	RCCGGY	1 cut(s)	387
Cfr13I	GGNCC	3 cut(s)	1243, 1483, 1892
Csp6I	GTAC	6 cut(s)	217, 844, 1096, 1121, 1649, 1742
CviQI	GTAC	6 cut(s)	217, 844, 1096, 1121, 1649, 1742
DdeI	CTNAG	3 cut(s)	1186, 1260, 1320
DraIII	CACNNNGTG	1 cut(s)	1377
EaeI	YGGCCR	3 cut(s)	36, 1059, 1100
EagI	CGGCCG	1 cut(s)	36
Eam1104I	CTCTTC	2 cut(s)	1013, 2061
EarI	CTCTTC	2 cut(s)	1013, 2061
EclXI	CGGCCG	1 cut(s)	36
Eco130I	CCWWGG	3 cut(s)	564, 839, 892
Eco31I	GGTCTC	1 cut(s)	1612
Eco47I	GGWCC	2 cut(s)	1483, 1892
Eco52I	CGGCCG	1 cut(s)	36
Eco57I	CTGAAG	5 cut(s)	332, 937, 945, 1722, 1949
Eco81I	CCTNAGG	1 cut(s)	1186
Eco88I	CYCGRG	3 cut(s)	220, 466, 2131
Eco91I	GGTNACC	1 cut(s)	1508
EcoO65I	GGTNACC	1 cut(s)	1508
EcoRI	GAATTC	1 cut(s)	1969
EcoRII	CCWGG	4 cut(s)	1048, 1239, 1443, 1660
EcoT14I	CCWWGG	3 cut(s)	564, 839, 892
EcoT22I	ATGCAT	1 cut(s)	599
ErhI	CCWWGG	3 cut(s)	564, 839, 892
FalI	AAGNNNNNCTT	2 cut(s)	305, 337
FaqI	GGGAC	5 cut(s)	203, 211, 733, 934, 1905
FauI	CCCGC	1 cut(s)	629
FauNDI	CATATG	1 cut(s)	1406
FbaI	TGATCA	3 cut(s)	801, 1383, 2097
FblI	GTMKAC	1 cut(s)	517
Fnu4HI	GCNGC	7 cut(s)	33, 36, 629, 879, 1128, 1530, 1775
FokI	GGATG	3 cut(s)	41, 266, 1643
Fsp4HI	GCNGC	7 cut(s)	33, 36, 629, 879, 1128, 1530, 1775
FspBI	CTAG	3 cut(s)	263, 650, 1206
GlaI	GCGC	1 cut(s)	65
GluI	GCNGC	7 cut(s)	33, 36, 629, 879, 1128, 1530, 1775
GsuI	CTGGAG	1 cut(s)	527
HaeIII	GGCC	6 cut(s)	38, 92, 1061, 1102, 1245, 1634
HhaI	GCGC	1 cut(s)	66
Hin6I	GCGC	1 cut(s)	64
HinP1I	GCGC	1 cut(s)	64
HindIII	AAGCTT	3 cut(s)	828, 1146, 2171
HinfI	GANTC	3 cut(s)	74, 1209, 1344
HphI	GGTGA	8 cut(s)	538, 628, 907, 1030, 1291, 1378, 1555, 1795
Hpy166II	GTNNAC	6 cut(s)	217, 518, 1222, 1570, 1744, 1921
Hpy188I	TCNGA	7 cut(s)	575, 1231, 1313, 1428, 1684, 1929, 2040
Hpy188III	TCNNGA	9 cut(s)	496, 688, 775, 850, 1213, 1259, 1597, 2086, 2119
Hpy8I	GTNNAC	6 cut(s)	217, 518, 1222, 1570, 1744, 1921
Hpy99I	CGWCG	1 cut(s)	1649
HpyCH4III	ACNGT	5 cut(s)	820, 1182, 1824, 1982, 2001
HpyCH4IV	ACGT	7 cut(s)	149, 481, 560, 1376, 1566, 1620, 1647
HpyCH4V	TGCA	8 cut(s)	334, 368, 395, 597, 628, 912, 1178, 1570
HpyF10VI	GCNNNNNNNGC	6 cut(s)	935, 1124, 1136, 1145, 1197, 1567
HpyF3I	CTNAG	3 cut(s)	1186, 1260, 1320
HpySE526I	ACGT	7 cut(s)	149, 481, 560, 1376, 1566, 1620, 1647
HspAI	GCGC	1 cut(s)	64
KroI	GCCGGC	1 cut(s)	387
KroNI	GCCGGC	1 cut(s)	389
Ksp22I	TGATCA	3 cut(s)	801, 1383, 2097
LmnI	GCTCC	5 cut(s)	53, 436, 751, 899, 1782
Lsp1109I	GCAGC	4 cut(s)	640, 865, 1114, 1516
LweI	GCATC	6 cut(s)	19, 51, 921, 1428, 1665, 1902
MaeI	CTAG	3 cut(s)	263, 650, 1206
MaeII	ACGT	7 cut(s)	149, 481, 560, 1376, 1566, 1620, 1647
MaeIII	GTNAC	5 cut(s)	645, 947, 1508, 1580, 1762
MbiI	CCGCTC	3 cut(s)	621, 636, 1777
MfeI	CAATTG	1 cut(s)	1628
MflI	RGATCY	1 cut(s)	1684
MhlI	GDGCHC	3 cut(s)	227, 1296, 1572
MlsI	TGGCCA	2 cut(s)	1061, 1102
MluCI	AATT	9 cut(s)	408, 445, 713, 789, 1326, 1493, 1628, 1969, 2091
MluNI	TGGCCA	2 cut(s)	1061, 1102
MlyI	GAGTC	2 cut(s)	68, 1218
MmeI	TCCRAC	4 cut(s)	119, 1752, 1806, 2164
Mox20I	TGGCCA	2 cut(s)	1061, 1102
Mph1103I	ATGCAT	1 cut(s)	599
MroNI	GCCGGC	1 cut(s)	387
MroXI	GAANNNNTTC	4 cut(s)	100, 830, 857, 1934
MscI	TGGCCA	2 cut(s)	1061, 1102
MseI	TTAA	4 cut(s)	81, 243, 407, 1637
MslI	CAYNNNNRTG	2 cut(s)	954, 1277
Msp20I	TGGCCA	2 cut(s)	1061, 1102
MspA1I	CMGCKG	1 cut(s)	614
MspR9I	CCNGG	7 cut(s)	919, 1050, 1241, 1445, 1662, 1781, 1896
MunI	CAATTG	1 cut(s)	1628
MvaI	CCWGG	4 cut(s)	1050, 1241, 1445, 1662
MvnI	CGCG	2 cut(s)	1013, 1349
MwoI	GCNNNNNNNGC	6 cut(s)	935, 1124, 1136, 1145, 1197, 1567
NaeI	GCCGGC	1 cut(s)	389
NciI	CCSGG	3 cut(s)	919, 1781, 1896
NcoI	CCATGG	2 cut(s)	564, 839
NdeI	CATATG	1 cut(s)	1406
NgoMIV	GCCGGC	1 cut(s)	387
NlaIV	GGNNCC	2 cut(s)	1830, 1893
NmuCI	GTSAC	3 cut(s)	645, 947, 1762
NsiI	ATGCAT	1 cut(s)	599
NspI	RCATGY	4 cut(s)	395, 1276, 1588, 1955
NspV	TTCGAA	1 cut(s)	1973
OliI	CACNNNNGTG	1 cut(s)	954
PaeI	GCATGC	1 cut(s)	395
PaeR7I	CTCGAG	3 cut(s)	220, 466, 2131
PciI	ACATGT	2 cut(s)	1584, 1951
PcsI	WCGNNNNNNNCGW	2 cut(s)	1116, 1695
PdiI	GCCGGC	1 cut(s)	389
PdmI	GAANNNNTTC	4 cut(s)	100, 830, 857, 1934
PfeI	GAWTC	1 cut(s)	1344
PkrI	GCNGC	7 cut(s)	34, 37, 630, 880, 1129, 1531, 1776
PleI	GAGTC	2 cut(s)	68, 1217
PpsI	GAGTC	2 cut(s)	68, 1217
Ppu21I	YACGTR	2 cut(s)	1567, 1621
PscI	ACATGT	2 cut(s)	1584, 1951
Psp1406I	AACGTT	1 cut(s)	481
Psp6I	CCWGG	4 cut(s)	1048, 1239, 1443, 1660
PspEI	GGTNACC	1 cut(s)	1508
PspGI	CCWGG	4 cut(s)	1048, 1239, 1443, 1660
PspN4I	GGNNCC	2 cut(s)	1830, 1893
PspPI	GGNCC	3 cut(s)	1243, 1483, 1892
PspXI	VCTCGAGB	3 cut(s)	220, 466, 2131
PstNI	CAGNNNCTG	1 cut(s)	1915
PsuI	RGATCY	1 cut(s)	1684
RsaI	GTAC	6 cut(s)	218, 845, 1097, 1122, 1650, 1743
RsaNI	GTAC	6 cut(s)	217, 844, 1096, 1121, 1649, 1742
RseI	CAYNNNNRTG	2 cut(s)	954, 1277
SaqAI	TTAA	4 cut(s)	81, 243, 407, 1637
SatI	GCNGC	7 cut(s)	33, 36, 629, 879, 1128, 1530, 1775
Sau96I	GGNCC	3 cut(s)	1243, 1483, 1892
SchI	GAGTC	2 cut(s)	68, 1218
ScrFI	CCNGG	7 cut(s)	919, 1050, 1241, 1445, 1662, 1781, 1896
SduI	GDGCHC	3 cut(s)	227, 1296, 1572
SfaNI	GCATC	6 cut(s)	19, 51, 921, 1428, 1665, 1902
Sfr274I	CTCGAG	3 cut(s)	220, 466, 2131
SfuI	TTCGAA	1 cut(s)	1973
SinI	GGWCC	2 cut(s)	1483, 1892
SlaI	CTCGAG	3 cut(s)	220, 466, 2131
SmiMI	CAYNNNNRTG	2 cut(s)	954, 1277
SmlI	CTYRAG	4 cut(s)	220, 466, 2119, 2131
SmoI	CTYRAG	4 cut(s)	220, 466, 2119, 2131
SphI	GCATGC	1 cut(s)	395
Sse9I	AATT	9 cut(s)	408, 445, 713, 789, 1326, 1493, 1628, 1969, 2091
SsiI	CCGC	8 cut(s)	32, 35, 614, 621, 636, 1349, 1506, 1775
SspI	AATATT	1 cut(s)	857
SspMI	CTAG	3 cut(s)	263, 650, 1206
StyD4I	CCNGG	7 cut(s)	917, 1048, 1239, 1443, 1660, 1779, 1894
StyI	CCWWGG	3 cut(s)	564, 839, 892
TaaI	ACNGT	5 cut(s)	820, 1182, 1824, 1982, 2001
TaiI	ACGT	7 cut(s)	152, 484, 563, 1379, 1569, 1623, 1650
TaqII	GACCGA	1 cut(s)	1471
TasI	AATT	9 cut(s)	408, 445, 713, 789, 1326, 1493, 1628, 1969, 2091
TatI	WGTACW	3 cut(s)	216, 1095, 1741
TauI	GCSGC	3 cut(s)	35, 38, 1777
TfiI	GAWTC	1 cut(s)	1344
Tru1I	TTAA	4 cut(s)	81, 243, 407, 1637
Tru9I	TTAA	4 cut(s)	81, 243, 407, 1637
TscAI	CASTG	4 cut(s)	21, 961, 1324, 1363
TseFI	GTSAC	3 cut(s)	645, 947, 1762
TseI	GCWGC	4 cut(s)	628, 878, 1127, 1529
Tsp45I	GTSAC	3 cut(s)	645, 947, 1762
TspDTI	ATGAA	5 cut(s)	110, 313, 1441, 1927, 1993
TspGWI	ACGGA	4 cut(s)	38, 449, 592, 1841
TspRI	CASTG	4 cut(s)	21, 961, 1324, 1363
VneI	GTGCAC	1 cut(s)	1568
VpaK11BI	GGWCC	2 cut(s)	1483, 1892
XapI	RAATTY	3 cut(s)	789, 1493, 1969
XceI	RCATGY	4 cut(s)	395, 1276, 1588, 1955
XcmI	CCANNNNNNNNNTGG	2 cut(s)	451, 1441
XhoI	CTCGAG	3 cut(s)	220, 466, 2131
XmiI	GTMKAC	1 cut(s)	517
XmnI	GAANNNNTTC	4 cut(s)	100, 830, 857, 1934
XspI	CTAG	3 cut(s)	263, 650, 1206
Zsp2I	ATGCAT	1 cut(s)	599

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.