Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004734

Physical Location & Seq

Genomic Coordinates Forward (+)

10161593 .. 10165234

Gene Structure 3642 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1133100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2187 bp

ATGGGAGAGAATTCGGAGACTACCGATGAACCGAGGATATCGATGCGTCGGGTAGACTCACTCAATTTGGAGGCTGGAAAAGTTTCCATGCCTAATCATCATGGCTCAAACATGAGTTGGAAGACAACGATCGGTTTAGCATTCCAGAGCATAGGGATAGTGTATGGGGACATCGGGACATCTCCGCTCTATGTATTCTCAAGCACTTTTCCTAATGGTATTGACCACAAGGATGACATTCTGGGGGTGTTGTCTCTCATCATCTATACCATATTGCTCGTACCCTTGGTTAAGTATGTCTTAATCGTCTTGTGGGCTAATGATAATGGTGAAGGGGGAACATTTGCGTTGTATTCGTTGATATGCCGGTATGCGAAGGTGAGCTTAATTCCAAATACGCAGCCAGAAGACAGAGAGCTATCCAACTACAAACTTGAAATTTCATCGGAGTTAAAAAGGTCCCAAGCGATAAAAAAGAAGCTTGAGAATAGTAAATATGCTAAATACACACTTTTCTTTATCACCATTATGGGAACTTCCATGGTGATTGGAGATGGGGTTCTTACTCCATGCATTTCAGTTCTTTCTGCAGTGAGCGGGATCAAGTCACTAGGCGCAAATGCTGTTGTGGGGATTTCGGTAGCAATCTTGATCGTTCTCTTCGCTGTTCAACAATTTGGGACTGATAAAGTGGGCTTCAGCTTTGCTCCTATTATCTTGTTGTGGTTCGCCTTCATCAGTGGCATCGGTCTTTACAACTTAATCGTATATGACGTTACTGTCTTACGTGCTTTCAATCCAGCTTACTTCATTTACTACTTCCATAGAAATGGAAAAAAGGCATGGATTTCCCTTGGGGGAGTAGTGCTCTGCATTACTGGGACCGAGGCCATGTTTGCTGATCTGGGTCACTTCAGTGTTCGAGCAATCCAACTTAGTTTCACTTGCTGCACATTTCCGGCAATACTTTTTGCATATTTTGGGCAAGCAGCATTTCTCACCAAGTTCCCGGACAAAGTGTCAGATACCTTCTACGCTTCAATTCCACACGCTATCTACTGGCCCACATTTGTGGTGGCCGTTTTAGCTGCCATTATTGCCAGTCAAGCTCTGATAACGGGGACATTTTCAATTATCTCCCAGTCCCTAAGTATGGGTTCTTTCCCTCGAGTAAAGATTGTTCACACCTCAGCAAAGAACGAGGGCCAAGTCTACATACCCGAGATTAACTACATACTTATGATTTTTTGTGTTATCATAACTGTGGCCTTCAAGACCACAGAGAAAATTGGCAATGCATATGGAATTGCGGTGGTTAGTGTGATGCTGATCACAACATGCATGCTCACTTTAATCATGCTAGTCATATGGAAGACAAGCATATTCTTGATTGCTATTTTCTTCGTGGTGTTCATTTTGATCGAAGGGGTTTATTTTTCTTCGGTTCTATTCAAGTTCATCGAAGGTGGTTACCTTCCGTTGTGCTTCGCCGCATTCCTAATGATGATTATGACGATCTGGCATTATGTGCACAAACAAAGTTACATTTATGAGCTCAACAACAAGGTGTCTAGTGAGTACATGAAGAAACTGGCCTCTAACCCTGACATTAAGCGCGTCCCAGGAATAGGGCTTTTGTATTCAGAACTTGTTCAAGGAATGCCTCCTATATTTGCTCATTTAGTTGCCAATATACCCTCAGTGCATTCAGTTGTGGTTGTTGTGACGATTAAGCCCCTACCGGTGAGCAAAGTGTTGTTGGAGGAGAGGTTTTTGTTTCGACAGTTGGAGCCCAGAGGGTACAGGATCTTCCGCTGTGTGGTGCGCAACGGTTACAACGATATAGTTGAGGATCCTGTGGAGTTTGAGAAGCAGCTGGTTGAGAATTTGAAAGATTTCATTCGCAATCAACAGATGTTAGATGAAGATCAAGCTGCAACGGATAGCCAGAACACTCGGGCAACGGCCGACGAGGAAATTGAGTTTGTGCAAGAGGCAATGGACAAAAGTACTGTGTATCTGATGGGAGAGACACAAGTGGTGGCAGGAGAGAAATCTTCCTGGTTCAAAAAGATTGTTGTCAACTACGTGTACGATTTCTTGAAGAAAAACTTTAGGCAAGCTGATAGCTTGATGGCAATTCCAAAGACCAGGCTTGTTAGGGTTGGAATGACCTATGAGATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0008150 GO:0008324 GO:0009674 GO:0009987 GO:0010107 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015291 GO:0015293 GO:0015294 GO:0015318 GO:0015370 GO:0015672 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0035725 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

729

Amino Acids

81.44

Weight (kDa)

8.15

Isoelectric Point (pI)

29.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	46 - 526	5.5e-165	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	541 - 728	1.1e-25	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004734)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13420
fragaria_vesca	FvH4_4g22240 FvH4_4g22240 FvH4_4g22260
malus_domestica	MD13G1133100.v1.1 MD13G1133200.v1.1 MD16G1143900.v1.1
prunus_persica	Prupe.1G212100_v2.0.a1 Prupe.1G212200_v2.0.a1
pyrus_communis	pycom13g11630
rosa_chinensis	RchiOBHm_Chr4g0428481 RchiOBHm_Chr4g0428501
rosa_laevigata	RLG00000007145 RLG00000007146
rosa_multiflora	Rmu_sc0002072.1_g000037 Rmu_sc0004623.1_g000012 Rmu_sc0006844.1_g000008
rosa_roxburghii	Rroxscaffold_5G00370180 Rroxscaffold_5G00370210
rosa_rugosa	Rorug04G0225500 Rorug04G0225600
rosa_samantha	Rh4AG283100 Rh4AG283200 Rh4BG288800 Rh4BG288900 Rh4CG304400 Rh4CG304900 Rh4DG285900 Rh4DG286000
rosa_wichuraiana	Rw4G024580 Rw4G024600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	779
Acc16I	TGCGCA	1 cut(s)	1826
AccBSI	CCGCTC	2 cut(s)	187, 597
AccI	GTMKAC	2 cut(s)	54, 1212
AccII	CGCG	1 cut(s)	1617
AciI	CCGC	5 cut(s)	185, 597, 1310, 1491, 1813
AclWI	GGATC	4 cut(s)	608, 1814, 1847, 1860
AcoI	YGGCCR	2 cut(s)	1077, 1965
AcsI	RAATTY	3 cut(s)	10, 438, 1885
AcuI	CTGAAG	2 cut(s)	682, 898
AfaI	GTAC	5 cut(s)	282, 1580, 1802, 2011, 2093
AfiI	CCNNNNNNNGG	3 cut(s)	1153, 1628, 1819
AflIII	ACRYGT	1 cut(s)	2088
AgeI	ACCGGT	1 cut(s)	1741
AhdI	GACNNNNNGTC	1 cut(s)	1018
AjnI	CCWGG	3 cut(s)	1621, 2060, 2150
AleI	CACNNNNGTG	2 cut(s)	915, 1070
AloI	GAACNNNNNNTCC	2 cut(s)	543, 575
Alw21I	GWGCWC	3 cut(s)	870, 1533, 1557
Alw26I	GTCTC	3 cut(s)	11, 258, 2024
Alw44I	GTGCAC	1 cut(s)	1529
AlwI	GGATC	4 cut(s)	608, 1814, 1847, 1860
Ama87I	CYCGRG	3 cut(s)	1167, 1220, 1956
AoxI	GGCC	7 cut(s)	888, 1061, 1077, 1204, 1266, 1593, 1965
ApaLI	GTGCAC	1 cut(s)	1529
ApeKI	GCWGC	6 cut(s)	400, 948, 989, 1088, 1873, 1934
ApoI	RAATTY	3 cut(s)	10, 438, 1885
AsiGI	ACCGGT	1 cut(s)	1741
Asp700I	GAANNNNTTC	3 cut(s)	82, 1650, 1895
AspLEI	GCGC	3 cut(s)	617, 1617, 1827
AspS9I	GGNCC	4 cut(s)	459, 882, 1062, 1204
AsuC2I	CCSGG	1 cut(s)	1010
AsuHPI	GGTGA	6 cut(s)	341, 391, 514, 556, 991, 1756
AvaI	CYCGRG	3 cut(s)	1167, 1220, 1956
AvaII	GGWCC	2 cut(s)	459, 882
BaeGI	GKGCMC	1 cut(s)	1533
BamHI	GGATCC	1 cut(s)	1852
BanII	GRGCYC	2 cut(s)	1557, 1794
BarI	GAAGNNNNNNTAC	2 cut(s)	1793, 1825
BbsI	GAAGAC	3 cut(s)	128, 414, 1379
Bbv12I	GWGCWC	3 cut(s)	870, 1533, 1557
BbvCI	CCTCAGC	1 cut(s)	1189
BbvI	GCAGC	6 cut(s)	412, 935, 1001, 1075, 1885, 1921
BccI	CCATC	3 cut(s)	548, 2017, 2128
BceAI	ACGGC	2 cut(s)	1064, 1980
BciT130I	CCWGG	3 cut(s)	1623, 2062, 2152
BclI	TGATCA	1 cut(s)	1329
BcnI	CCSGG	1 cut(s)	1010
BcoDI	GTCTC	3 cut(s)	11, 258, 2024
BfaI	CTAG	3 cut(s)	611, 1361, 1572
BfmI	CTRYAG	1 cut(s)	588
BisI	GCNGC	7 cut(s)	401, 949, 990, 1089, 1491, 1874, 1935
BlsI	GCNGC	7 cut(s)	402, 950, 991, 1090, 1492, 1875, 1936
BmcAI	AGTACT	1 cut(s)	2011
Bme1390I	CCNGG	4 cut(s)	1010, 1623, 2062, 2152
Bme18I	GGWCC	2 cut(s)	459, 882
BmeRI	GACNNNNNGTC	1 cut(s)	1018
BmeT110I	CYCGRG	3 cut(s)	1167, 1220, 1956
BmgT120I	GGNCC	4 cut(s)	459, 882, 1062, 1204
BmiI	GGNNCC	4 cut(s)	461, 883, 1791, 1854
BmrFI	CCNGG	4 cut(s)	1010, 1623, 2062, 2152
BmrI	ACTGGG	2 cut(s)	888, 1135
BmsI	GCATC	3 cut(s)	33, 753, 1314
BmuI	ACTGGG	2 cut(s)	888, 1135
BpiI	GAAGAC	3 cut(s)	128, 414, 1379
BplI	GAGNNNNNCTC	2 cut(s)	852, 884
Bpu10I	CCTNAGC	1 cut(s)	1189
BpuEI	CTTGAG	2 cut(s)	184, 503
BpuMI	CCSGG	1 cut(s)	1010
Bsa29I	ATCGAT	1 cut(s)	41
BsaAI	YACGTR	2 cut(s)	788, 2089
BsaBI	GATNNNNATC	2 cut(s)	1328, 1926
BsaJI	CCNNGG	6 cut(s)	32, 285, 540, 853, 885, 1621
BsaWI	WCCGGW	1 cut(s)	1741
BsaXI	ACNNNNNCTCC	4 cut(s)	543, 573, 1754, 1784
Bsc4I	CCNNNNNNNGG	3 cut(s)	1153, 1628, 1819
Bse118I	RCCGGY	2 cut(s)	366, 1741
Bse1I	ACTGG	5 cut(s)	883, 1064, 1101, 1141, 1596
Bse3DI	GCAATG	2 cut(s)	1300, 2004
Bse8I	GATNNNNATC	2 cut(s)	1328, 1926
BseBI	CCWGG	3 cut(s)	1623, 2062, 2152
BseCI	ATCGAT	1 cut(s)	41
BseDI	CCNNGG	6 cut(s)	32, 285, 540, 853, 885, 1621
BseGI	GGATG	1 cut(s)	238
BseJI	GATNNNNATC	2 cut(s)	1328, 1926
BseLI	CCNNNNNNNGG	3 cut(s)	1153, 1628, 1819
BseMI	GCAATG	2 cut(s)	1300, 2004
BseMII	CTCAG	2 cut(s)	1203, 1713
BseNI	ACTGG	5 cut(s)	883, 1064, 1101, 1141, 1596
BseRI	GAGGAG	1 cut(s)	1778
BseSI	GKGCMC	1 cut(s)	1533
BseX3I	CGGCCG	1 cut(s)	1965
BseXI	GCAGC	6 cut(s)	412, 935, 1001, 1075, 1885, 1921
BsgI	GTGCAG	1 cut(s)	934
Bsh1236I	CGCG	1 cut(s)	1617
Bsh1285I	CGRYCG	2 cut(s)	132, 1968
BshFI	GGCC	7 cut(s)	890, 1063, 1079, 1206, 1268, 1595, 1967
BshTI	ACCGGT	1 cut(s)	1741
BshVI	ATCGAT	1 cut(s)	41
BsiEI	CGRYCG	2 cut(s)	132, 1968
BsiHKAI	GWGCWC	3 cut(s)	870, 1533, 1557
BsiHKCI	CYCGRG	3 cut(s)	1167, 1220, 1956
BsiSI	CCGG	4 cut(s)	367, 959, 1010, 1742
BslFI	GGGAC	8 cut(s)	182, 190, 445, 694, 895, 1129, 1135, 1604
BslI	CCNNNNNNNGG	3 cut(s)	1153, 1628, 1819
BsmAI	GTCTC	3 cut(s)	11, 258, 2024
BsmFI	GGGAC	8 cut(s)	182, 190, 445, 694, 895, 1129, 1135, 1604
BsmI	GAATGC	4 cut(s)	140, 1493, 1665, 1705
BsnI	GGCC	7 cut(s)	890, 1063, 1079, 1206, 1268, 1595, 1967
BsoBI	CYCGRG	3 cut(s)	1167, 1220, 1956
Bsp1286I	GDGCHC	4 cut(s)	870, 1533, 1557, 1794
Bsp19I	CCATGG	1 cut(s)	540
BspACI	CCGC	5 cut(s)	185, 597, 1310, 1491, 1813
BspANI	GGCC	7 cut(s)	890, 1063, 1079, 1206, 1268, 1595, 1967
BspCNI	CTCAG	2 cut(s)	1202, 1712
BspDI	ATCGAT	1 cut(s)	41
BspFNI	CGCG	1 cut(s)	1617
BspLI	GGNNCC	4 cut(s)	461, 883, 1791, 1854
BspMAI	CTGCAG	1 cut(s)	592
BspPI	GGATC	4 cut(s)	608, 1814, 1847, 1860
BsrBI	CCGCTC	2 cut(s)	187, 597
BsrDI	GCAATG	2 cut(s)	1300, 2004
BsrFI	RCCGGY	2 cut(s)	366, 1741
BsrI	ACTGG	5 cut(s)	883, 1064, 1101, 1141, 1596
BssAI	RCCGGY	2 cut(s)	366, 1741
BssECI	CCNNGG	6 cut(s)	32, 285, 540, 853, 885, 1621
BssT1I	CCWWGG	3 cut(s)	285, 540, 853
Bst2UI	CCWGG	3 cut(s)	1623, 2062, 2152
Bst4CI	ACNGT	5 cut(s)	781, 1264, 1785, 1832, 2014
Bst6I	CTCTTC	1 cut(s)	665
BstAPI	GCANNNNNTGC	1 cut(s)	1528
BstBAI	YACGTR	2 cut(s)	788, 2089
BstC8I	GCNNGC	3 cut(s)	987, 1343, 2121
BstDEI	CTNAG	4 cut(s)	935, 1148, 1189, 1699
BstDSI	CCRYGG	1 cut(s)	540
BstEII	GGTNACC	1 cut(s)	1469
BstF5I	GGATG	1 cut(s)	238
BstFNI	CGCG	1 cut(s)	1617
BstHHI	GCGC	3 cut(s)	617, 1617, 1827
BstMAI	GTCTC	3 cut(s)	11, 258, 2024
BstMCI	CGRYCG	2 cut(s)	132, 1968
BstMWI	GCNNNNNNNGC	5 cut(s)	896, 1085, 1097, 1106, 1528
BstNI	CCWGG	3 cut(s)	1623, 2062, 2152
BstNSI	RCATGY	2 cut(s)	1341, 1345
BstPI	GGTNACC	1 cut(s)	1469
BstSCI	CCNGG	4 cut(s)	1008, 1621, 2060, 2150
BstSFI	CTRYAG	1 cut(s)	588
BstSLI	GKGCMC	1 cut(s)	1533
BstUI	CGCG	1 cut(s)	1617
BstV1I	GCAGC	6 cut(s)	412, 935, 1001, 1075, 1885, 1921
BstV2I	GAAGAC	3 cut(s)	128, 414, 1379
BstX2I	RGATCY	2 cut(s)	1806, 1852
BstXI	CCANNNNNNTGG	2 cut(s)	830, 1072
BstYI	RGATCY	2 cut(s)	1806, 1852
BstZI	CGGCCG	1 cut(s)	1965
Bsu15I	ATCGAT	1 cut(s)	41
BsuRI	GGCC	7 cut(s)	890, 1063, 1079, 1206, 1268, 1595, 1967
BsuTUI	ATCGAT	1 cut(s)	41
BtgI	CCRYGG	1 cut(s)	540
BtsCI	GGATG	1 cut(s)	238
BtsI	GCAGTG	1 cut(s)	597
BtsIMutI	CAGTG	4 cut(s)	597, 745, 922, 1707
Cac8I	GCNNGC	3 cut(s)	987, 1343, 2121
CfoI	GCGC	3 cut(s)	617, 1617, 1827
Cfr10I	RCCGGY	2 cut(s)	366, 1741
Cfr13I	GGNCC	4 cut(s)	459, 882, 1062, 1204
ClaI	ATCGAT	1 cut(s)	41
CseI	GACGC	2 cut(s)	35, 1606
Csp6I	GTAC	5 cut(s)	281, 1579, 1801, 2010, 2092
CspAI	ACCGGT	1 cut(s)	1741
CviQI	GTAC	5 cut(s)	281, 1579, 1801, 2010, 2092
DdeI	CTNAG	4 cut(s)	935, 1148, 1189, 1699
DrdI	GACNNNNNNGTC	1 cut(s)	779
DriI	GACNNNNNGTC	1 cut(s)	1018
DseDI	GACNNNNNNGTC	1 cut(s)	779
EaeI	YGGCCR	2 cut(s)	1077, 1965
EagI	CGGCCG	1 cut(s)	1965
Eam1104I	CTCTTC	1 cut(s)	665
Eam1105I	GACNNNNNGTC	1 cut(s)	1018
EarI	CTCTTC	1 cut(s)	665
Ecl136II	GAGCTC	1 cut(s)	1555
EclXI	CGGCCG	1 cut(s)	1965
Eco130I	CCWWGG	3 cut(s)	285, 540, 853
Eco24I	GRGCYC	2 cut(s)	1557, 1794
Eco32I	GATATC	1 cut(s)	39
Eco47I	GGWCC	2 cut(s)	459, 882
Eco52I	CGGCCG	1 cut(s)	1965
Eco53kI	GAGCTC	1 cut(s)	1555
Eco57I	CTGAAG	2 cut(s)	682, 898
Eco88I	CYCGRG	3 cut(s)	1167, 1220, 1956
Eco91I	GGTNACC	1 cut(s)	1469
EcoICRI	GAGCTC	1 cut(s)	1555
EcoO109I	RGGNCCY	1 cut(s)	459
EcoO65I	GGTNACC	1 cut(s)	1469
EcoRI	GAATTC	1 cut(s)	10
EcoRII	CCWGG	3 cut(s)	1621, 2060, 2150
EcoRV	GATATC	1 cut(s)	39
EcoT14I	CCWWGG	3 cut(s)	285, 540, 853
EcoT22I	ATGCAT	3 cut(s)	575, 1300, 1343
EcoT38I	GRGCYC	2 cut(s)	1557, 1794
ErhI	CCWWGG	3 cut(s)	285, 540, 853
FalI	AAGNNNNNCTT	6 cut(s)	284, 316, 368, 400, 2096, 2128
FaqI	GGGAC	8 cut(s)	182, 190, 445, 694, 895, 1129, 1135, 1604
FauI	CCCGC	1 cut(s)	590
FauNDI	CATATG	2 cut(s)	1300, 1367
FbaI	TGATCA	1 cut(s)	1329
FblI	GTMKAC	2 cut(s)	54, 1212
Fnu4HI	GCNGC	7 cut(s)	401, 949, 990, 1089, 1491, 1874, 1935
FokI	GGATG	1 cut(s)	245
FriOI	GRGCYC	2 cut(s)	1557, 1794
Fsp4HI	GCNGC	7 cut(s)	401, 949, 990, 1089, 1491, 1874, 1935
FspBI	CTAG	3 cut(s)	611, 1361, 1572
FspI	TGCGCA	1 cut(s)	1826
GlaI	GCGC	3 cut(s)	616, 1616, 1826
GluI	GCNGC	7 cut(s)	401, 949, 990, 1089, 1491, 1874, 1935
HaeIII	GGCC	7 cut(s)	890, 1063, 1079, 1206, 1268, 1595, 1967
HapII	CCGG	4 cut(s)	367, 959, 1010, 1742
HgaI	GACGC	2 cut(s)	35, 1606
HhaI	GCGC	3 cut(s)	617, 1617, 1827
Hin6I	GCGC	3 cut(s)	615, 1615, 1825
HinP1I	GCGC	3 cut(s)	615, 1615, 1825
HincII	GTYRAC	1 cut(s)	2083
HindII	GTYRAC	1 cut(s)	2083
HindIII	AAGCTT	1 cut(s)	479
HinfI	GANTC	1 cut(s)	56
HpaII	CCGG	4 cut(s)	367, 959, 1010, 1742
HphI	GGTGA	6 cut(s)	341, 391, 514, 556, 991, 1756
Hpy166II	GTNNAC	6 cut(s)	55, 1183, 1213, 1531, 2083, 2092
Hpy188I	TCNGA	6 cut(s)	16, 448, 1024, 1113, 1645, 2022
Hpy188III	TCNNGA	6 cut(s)	145, 175, 649, 1273, 1387, 2101
Hpy8I	GTNNAC	6 cut(s)	55, 1183, 1213, 1531, 2083, 2092
Hpy99I	CGWCG	2 cut(s)	51, 1973
HpyAV	CCTTC	8 cut(s)	326, 370, 742, 1039, 1279, 1418, 1457, 1484
HpyCH4III	ACNGT	5 cut(s)	781, 1264, 1785, 1832, 2014
HpyCH4IV	ACGT	3 cut(s)	774, 787, 2088
HpyF10VI	GCNNNNNNNGC	5 cut(s)	896, 1085, 1097, 1106, 1528
HpyF3I	CTNAG	4 cut(s)	935, 1148, 1189, 1699
HpySE526I	ACGT	3 cut(s)	774, 787, 2088
HspAI	GCGC	3 cut(s)	615, 1615, 1825
Ksp22I	TGATCA	1 cut(s)	1329
LmnI	GCTCC	2 cut(s)	712, 1789
Lsp1109I	GCAGC	6 cut(s)	412, 935, 1001, 1075, 1885, 1921
LweI	GCATC	3 cut(s)	33, 753, 1314
MaeI	CTAG	3 cut(s)	611, 1361, 1572
MaeII	ACGT	3 cut(s)	774, 787, 2088
MaeIII	GTNAC	7 cut(s)	606, 775, 908, 1469, 1541, 1723, 1832
MbiI	CCGCTC	2 cut(s)	187, 597
MflI	RGATCY	2 cut(s)	1806, 1852
MhlI	GDGCHC	4 cut(s)	870, 1533, 1557, 1794
MlyI	GAGTC	1 cut(s)	50
MmeI	TCCRAC	6 cut(s)	98, 447, 955, 1740, 1767, 2146
Mph1103I	ATGCAT	3 cut(s)	575, 1300, 1343
MroXI	GAANNNNTTC	3 cut(s)	82, 1650, 1895
MseI	TTAA	9 cut(s)	291, 302, 386, 452, 761, 1227, 1352, 1611, 1731
MslI	CAYNNNNRTG	7 cut(s)	231, 527, 828, 915, 1070, 1238, 1262
MspA1I	CMGCKG	2 cut(s)	1815, 1876
MspI	CCGG	4 cut(s)	367, 959, 1010, 1742
MspR9I	CCNGG	4 cut(s)	1010, 1623, 2062, 2152
Mva1269I	GAATGC	4 cut(s)	140, 1493, 1665, 1705
MvaI	CCWGG	3 cut(s)	1623, 2062, 2152
MvnI	CGCG	1 cut(s)	1617
MwoI	GCNNNNNNNGC	5 cut(s)	896, 1085, 1097, 1106, 1528
NciI	CCSGG	1 cut(s)	1010
NcoI	CCATGG	1 cut(s)	540
NdeI	CATATG	2 cut(s)	1300, 1367
NlaIV	GGNNCC	4 cut(s)	461, 883, 1791, 1854
NmuCI	GTSAC	3 cut(s)	606, 908, 1723
NsbI	TGCGCA	1 cut(s)	1826
NsiI	ATGCAT	3 cut(s)	575, 1300, 1343
NspI	RCATGY	2 cut(s)	1341, 1345
OliI	CACNNNNGTG	2 cut(s)	915, 1070
PaeI	GCATGC	1 cut(s)	1345
PaeR7I	CTCGAG	1 cut(s)	1167
PcsI	WCGNNNNNNNCGW	2 cut(s)	771, 1836
PctI	GAATGC	4 cut(s)	140, 1493, 1665, 1705
PdmI	GAANNNNTTC	3 cut(s)	82, 1650, 1895
PfoI	TCCNGGA	1 cut(s)	1008
PinAI	ACCGGT	1 cut(s)	1741
PkrI	GCNGC	7 cut(s)	402, 950, 991, 1090, 1492, 1875, 1936
Ple19I	CGATCG	1 cut(s)	132
PleI	GAGTC	1 cut(s)	50
PpsI	GAGTC	1 cut(s)	50
Ppu21I	YACGTR	2 cut(s)	788, 2089
PpuMI	RGGWCCY	1 cut(s)	459
Psp124BI	GAGCTC	1 cut(s)	1557
Psp5II	RGGWCCY	1 cut(s)	459
Psp6I	CCWGG	3 cut(s)	1621, 2060, 2150
PspEI	GGTNACC	1 cut(s)	1469
PspGI	CCWGG	3 cut(s)	1621, 2060, 2150
PspN4I	GGNNCC	4 cut(s)	461, 883, 1791, 1854
PspPI	GGNCC	4 cut(s)	459, 882, 1062, 1204
PspPPI	RGGWCCY	1 cut(s)	459
PspXI	VCTCGAGB	1 cut(s)	1167
PsrI	GAACNNNNNNTAC	2 cut(s)	1164, 1196
PstI	CTGCAG	1 cut(s)	592
PsuI	RGATCY	2 cut(s)	1806, 1852
PvuI	CGATCG	1 cut(s)	132
PvuII	CAGCTG	1 cut(s)	1876
RsaI	GTAC	5 cut(s)	282, 1580, 1802, 2011, 2093
RsaNI	GTAC	5 cut(s)	281, 1579, 1801, 2010, 2092
RseI	CAYNNNNRTG	7 cut(s)	231, 527, 828, 915, 1070, 1238, 1262
SacI	GAGCTC	1 cut(s)	1557
SaqAI	TTAA	9 cut(s)	291, 302, 386, 452, 761, 1227, 1352, 1611, 1731
SatI	GCNGC	7 cut(s)	401, 949, 990, 1089, 1491, 1874, 1935
Sau96I	GGNCC	4 cut(s)	459, 882, 1062, 1204
ScaI	AGTACT	1 cut(s)	2011
SchI	GAGTC	1 cut(s)	50
ScrFI	CCNGG	4 cut(s)	1010, 1623, 2062, 2152
SduI	GDGCHC	4 cut(s)	870, 1533, 1557, 1794
SfaNI	GCATC	3 cut(s)	33, 753, 1314
SfcI	CTRYAG	1 cut(s)	588
Sfr274I	CTCGAG	1 cut(s)	1167
SinI	GGWCC	2 cut(s)	459, 882
SlaI	CTCGAG	1 cut(s)	1167
SmiMI	CAYNNNNRTG	7 cut(s)	231, 527, 828, 915, 1070, 1238, 1262
SmlI	CTYRAG	3 cut(s)	199, 482, 1167
SmoI	CTYRAG	3 cut(s)	199, 482, 1167
SphI	GCATGC	1 cut(s)	1345
SsiI	CCGC	5 cut(s)	185, 597, 1310, 1491, 1813
SspMI	CTAG	3 cut(s)	611, 1361, 1572
SstI	GAGCTC	1 cut(s)	1557
StyD4I	CCNGG	4 cut(s)	1008, 1621, 2060, 2150
StyI	CCWWGG	3 cut(s)	285, 540, 853
TaaI	ACNGT	5 cut(s)	781, 1264, 1785, 1832, 2014
TaiI	ACGT	3 cut(s)	777, 790, 2091
TaqI	TCGA	6 cut(s)	41, 922, 1168, 1422, 1461, 1780
TaqII	GACCGA	2 cut(s)	737, 899
TatI	WGTACW	2 cut(s)	1578, 2009
TauI	GCSGC	1 cut(s)	1493
Tru1I	TTAA	9 cut(s)	291, 302, 386, 452, 761, 1227, 1352, 1611, 1731
Tru9I	TTAA	9 cut(s)	291, 302, 386, 452, 761, 1227, 1352, 1611, 1731
TscAI	CASTG	4 cut(s)	597, 745, 922, 1707
TseFI	GTSAC	3 cut(s)	606, 908, 1723
TseI	GCWGC	6 cut(s)	400, 948, 989, 1088, 1873, 1934
Tsp45I	GTSAC	3 cut(s)	606, 908, 1723
TspDTI	ATGAA	9 cut(s)	42, 432, 724, 799, 1402, 1447, 1598, 1888, 1938
TspGWI	ACGGA	2 cut(s)	1467, 1955
TspRI	CASTG	4 cut(s)	597, 745, 922, 1707
VneI	GTGCAC	1 cut(s)	1529
VpaK11BI	GGWCC	2 cut(s)	459, 882
XapI	RAATTY	3 cut(s)	10, 438, 1885
XceI	RCATGY	2 cut(s)	1341, 1345
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1072
XhoI	CTCGAG	1 cut(s)	1167
XmiI	GTMKAC	2 cut(s)	54, 1212
XmnI	GAANNNNTTC	3 cut(s)	82, 1650, 1895
XspI	CTAG	3 cut(s)	611, 1361, 1572
ZrmI	AGTACT	1 cut(s)	2011
Zsp2I	ATGCAT	3 cut(s)	575, 1300, 1343

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.