Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004734

Physical Location & Seq

Genomic Coordinates Forward (+)

11053616 .. 11062225

Gene Structure 8610 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1143900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2370 bp

ATGGCGGAGAAAGTGGTGATCCTGACAGAGGGGGAGACAGAAGAAGCAAAAGAAATAACGAACCAGCAACTGAAGGAGAGAAAAGGATCATGGGCAAAGTTGCGCCATGTCGACTCTCTCAATTTGGAGGCCGGAAGAGTGTCCATGAATGGAAGGCACGGCTCACAGGTAAATAACTGGCAGAGAACATTGAGTTTGGCATTTCAGAGCATGGGGATTGTGTATGGGGACATAGGGACGTCTCCGCTCTATGTGTATGCTAGCACTTTCACTGATGGTATTGACAACACTGACGATGTTATAGGAGTATTGTCTCTCATCATCTATACCATAGCGCTCGTACCCCTACTTAAATATGTCTTCATCGTGCTATGGGCTAATGACAATGGTGATGGGGGAACATTTGCACTGTATTCCTTGATGTGCCGACATGCAAAGGTGAGCTTAACTCCAAATAACCAACCAGAAGACAGGGAGCTATCTAACTACAAACTTGAAACACCGTCCAACGAGTTAAAACGATCCCAAACCATCAAGAAGAAGCTCGAGAATAGTAAGATTGCCAAATACATCCTTTTCCTTGTCACCATTATGGGAACTTCAATGGTTATTGGAGATGGGGTTCTTACTCCTTGCATTTCAGTTCTTTCTGCTGTGAGCGGGATCAAGTCACTAGGCTCAGATGCTGTTGTGGGGATTTCTATAGCAATCTTGATCTTACTGTTTGCTGTTCAACAATTTGGCACTGATAAAGTTGGATTCACATTTGCACCTATTATCCTGGTGTGGTTCATGTTCATCGGTGGCATCGGACTTTACAACTTATTCAAATATGACATTGGTGTATTACGTGCATTCAATCCAAAATACATCATCGATTTCTTTAGAAGAAATGGGAAAAAGGGATGGGTATCCCTTGGAGGAATATTTCTCTGCGTTACTGGGACGGAGGCCATGTTTGCTGATCTGGGTCACTTCAGTGTCAAAGCAATCCAAGTGAGTTTCTCTTGCATCACATTTCCTGCATTAATAATTGCATATAGTGGGCAAGCAGCGTTCCTCAGAAAGTTCCCAGGGAAGGTGGAGAACACATTCTATGACTCAATACCAGACCCTTTGTACTGGCCAACGTTCATTGTGGCGGTAGCAGCTGCCATCATTGCCAGCCAAGCTATGATAACTGGAGCATTTTCAATTATCTCTCAATCCCTAAGTTTGGGTTGTTTTCCAAGAGTTAAGGTTGTTCATACCTCTGCAAAGTATGAGGGTCAAGTATACATACCTGAGATCAACTACCTGCTCATGGTTGCTTGTGTAATAGTCACTGCAGCCTTTAAGACCACGGAAAAAATTGGCAATGCGTATGGAATTGCAGTGGTTAGTGTTATGGTGATAACAACATGTTTGCTTACTCTAATAATGCTAGTGATATGGAAAACTAGCATATGGTTGATTGGTCTGTTCTTTATGGTGTTCTTTTGTATCGAGGTGGTTTATGCATCTGCGGTGATGTACAAATTCATTCACGGCGGTTACCTTCCTCTGGTCTTTTCTTTTTTCCTTATGGTAATCATGGGGATTTGGCATTATGTGCACCATCAAAGATACATGTTCGAGCTCAAGAATAAGGTTTCTAGTGAATACATGAAACAATTGGCCTCTGACCCCAACATAAACCGTGTGCCAGGAATTGGACTTCTATATTCTGAGCTTGTGCAAGGGATTCCCCCTATATTTTCTCACTTTGTTAACAACATACCGTCCATCCACTCTCTTATAGTTATTGTGTCGATCAAACCTATTCCATTCAGCAAAGTGGCATTGGAGGAAAGGTTTCTATTTCGACAGTTGGAGCCAAGAGATTACCGAATGTTCCGTTGTGTAGCTAGGTATGGTTACAATGACAGAATGGAGGAACCCAAAGAGTTTGAGGAACAACTAGTTGAGAATTTGAAAGAATTTATCCACCATGAGCACTTTGCTCATGAAGGAGAAATAACAAACGAGGCAGTTTCAACTGGACAGTCATTCAATGCTGAAGAATCAGTTCAACAAGTTAACCAACCTCGTCTTTCATCTGGGCGCATTCTATCATTCAATACTGCTAGGTCAACATCTAATCGAATAGTCTCTGCACCCGTCAAAGGAGCTCCCGAGGAAATGCAGTTTGTGCAAGAGGCTATGGAGAAAGGCATAGTTTATCTGCTGGGAGAGACAGAAGTGGTGGCAGAAGCGAACTCGTCATTGTTCAAGAGGATAGTTGTCAACCATGTCTACAGTTTCTTGAGGAAAAACTTTAGGCAAGGGGAGCAAATCATGAGAATCCCACGGACTAGGCTTCTCAGGGTTGGAATGACCTATGAGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0008150 GO:0008324 GO:0009674 GO:0009987 GO:0010107 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015291 GO:0015293 GO:0015294 GO:0015318 GO:0015370 GO:0015672 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0035725 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

790

Amino Acids

88.55

Weight (kDa)

8.58

Isoelectric Point (pI)

27.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	66 - 548	3.3e-168	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	562 - 789	4.1e-25	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004734)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13420
fragaria_vesca	FvH4_4g22240 FvH4_4g22240 FvH4_4g22260
malus_domestica	MD13G1133100.v1.1 MD13G1133200.v1.1 MD16G1143900.v1.1
prunus_persica	Prupe.1G212100_v2.0.a1 Prupe.1G212200_v2.0.a1
pyrus_communis	pycom13g11630
rosa_chinensis	RchiOBHm_Chr4g0428481 RchiOBHm_Chr4g0428501
rosa_laevigata	RLG00000007145 RLG00000007146
rosa_multiflora	Rmu_sc0002072.1_g000037 Rmu_sc0004623.1_g000012 Rmu_sc0006844.1_g000008
rosa_roxburghii	Rroxscaffold_5G00370180 Rroxscaffold_5G00370210
rosa_rugosa	Rorug04G0225500 Rorug04G0225600
rosa_samantha	Rh4AG283100 Rh4AG283200 Rh4BG288800 Rh4BG288900 Rh4CG304400 Rh4CG304900 Rh4DG285900 Rh4DG286000
rosa_wichuraiana	Rw4G024580 Rw4G024600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	242
Acc36I	ACCTGC	1 cut(s)	1305
AccB7I	CCANNNNNTGG	1 cut(s)	1691
AccBSI	CCGCTC	2 cut(s)	247, 660
AccI	GTMKAC	3 cut(s)	111, 1275, 2274
AciI	CCGC	6 cut(s)	5, 245, 660, 1142, 1505, 1530
AclI	AACGTT	1 cut(s)	1130
AclWI	GGATC	4 cut(s)	13, 94, 516, 671
AcoI	YGGCCR	1 cut(s)	1124
AcsI	RAATTY	3 cut(s)	1517, 1948, 1958
AcuI	CTGAAG	3 cut(s)	92, 961, 2058
AcyI	GRCGYC	1 cut(s)	239
AfaI	GTAC	3 cut(s)	342, 1121, 1514
AfeI	AGCGCT	1 cut(s)	336
AfiI	CCNNNNNNNGG	7 cut(s)	28, 1078, 1216, 1303, 1543, 1691, 2144
AflIII	ACRYGT	2 cut(s)	1400, 1608
AhlI	ACTAGT	1 cut(s)	1939
AjnI	CCWGG	3 cut(s)	780, 1072, 1684
AleI	CACNNNNGTG	1 cut(s)	978
AloI	GAACNNNNNNTCC	2 cut(s)	606, 638
AluBI	AGCT	9 cut(s)	444, 478, 544, 1151, 1172, 1618, 1711, 1886, 2150
AluI	AGCT	9 cut(s)	444, 478, 544, 1151, 1172, 1618, 1711, 1886, 2150
Alw21I	GWGCWC	4 cut(s)	1596, 1620, 1977, 2152
Alw26I	GTCTC	5 cut(s)	29, 246, 318, 2134, 2207
Alw44I	GTGCAC	1 cut(s)	1592
AlwI	GGATC	4 cut(s)	13, 94, 516, 671
AlwNI	CAGNNNCTG	2 cut(s)	70, 686
Ama87I	CYCGRG	2 cut(s)	545, 2153
Aor51HI	AGCGCT	1 cut(s)	336
AoxI	GGCC	4 cut(s)	129, 951, 1124, 1656
ApaLI	GTGCAC	1 cut(s)	1592
ApeKI	GCWGC	4 cut(s)	1052, 1148, 1151, 1328
ApoI	RAATTY	3 cut(s)	1517, 1948, 1958
AseI	ATTAAT	1 cut(s)	1028
Asp700I	GAANNNNTTC	3 cut(s)	1091, 1833, 2046
AspLEI	GCGC	3 cut(s)	105, 337, 2085
AsuHPI	GGTGA	6 cut(s)	28, 401, 451, 577, 1402, 1519
AsuNHI	GCTAGC	1 cut(s)	260
AvaI	CYCGRG	2 cut(s)	545, 2153
BaeGI	GKGCMC	1 cut(s)	1596
BalI	TGGCCA	1 cut(s)	1126
BanII	GRGCYC	2 cut(s)	1620, 2152
BbsI	GAAGAC	2 cut(s)	352, 474
Bbv12I	GWGCWC	4 cut(s)	1596, 1620, 1977, 2152
BbvI	GCAGC	4 cut(s)	1064, 1138, 1160, 1340
BccI	CCATC	8 cut(s)	269, 386, 539, 611, 900, 1163, 1605, 1772
BceAI	ACGGC	2 cut(s)	175, 1543
BciT130I	CCWGG	3 cut(s)	782, 1074, 1686
BciVI	GTATCC	1 cut(s)	922
BcoDI	GTCTC	5 cut(s)	29, 246, 318, 2134, 2207
BcuI	ACTAGT	1 cut(s)	1939
BfaI	CTAG	9 cut(s)	261, 674, 1424, 1440, 1635, 1887, 1940, 2106, 2334
BfmI	CTRYAG	3 cut(s)	702, 1326, 2275
BfoI	RGCGCY	1 cut(s)	338
BfuAI	ACCTGC	1 cut(s)	1305
BfuI	GTATCC	1 cut(s)	922
BisI	GCNGC	4 cut(s)	1053, 1149, 1152, 1329
BlsI	GCNGC	4 cut(s)	1054, 1150, 1153, 1330
Bme1390I	CCNGG	3 cut(s)	782, 1074, 1686
BmeT110I	CYCGRG	2 cut(s)	545, 2153
BmiI	GGNNCC	2 cut(s)	1854, 1917
BmrFI	CCNGG	3 cut(s)	782, 1074, 1686
BmrI	ACTGGG	1 cut(s)	951
BmsI	GCATC	4 cut(s)	673, 816, 1020, 1508
BmtI	GCTAGC	1 cut(s)	264
BmuI	ACTGGG	1 cut(s)	951
BpiI	GAAGAC	2 cut(s)	352, 474
BplI	GAGNNNNNCTC	2 cut(s)	433, 465
BpmI	CTGGAG	1 cut(s)	1203
BpuEI	CTTGAG	2 cut(s)	1604, 2305
Bsa29I	ATCGAT	1 cut(s)	876
BsaAI	YACGTR	1 cut(s)	851
BsaBI	GATNNNNATC	1 cut(s)	910
BsaHI	GRCGYC	1 cut(s)	239
BsaJI	CCNNGG	6 cut(s)	916, 1072, 1073, 1341, 2154, 2327
BsaXI	ACNNNNNCTCC	6 cut(s)	606, 636, 941, 971, 1817, 1847
Bsc4I	CCNNNNNNNGG	7 cut(s)	28, 1078, 1216, 1303, 1543, 1691, 2144
Bse1I	ACTGG	5 cut(s)	182, 946, 1127, 1186, 2023
Bse3DI	GCAATG	2 cut(s)	1158, 1363
Bse8I	GATNNNNATC	1 cut(s)	910
BseBI	CCWGG	3 cut(s)	782, 1074, 1686
BseCI	ATCGAT	1 cut(s)	876
BseDI	CCNNGG	6 cut(s)	916, 1072, 1073, 1341, 2154, 2327
BseGI	GGATG	3 cut(s)	570, 911, 1764
BseJI	GATNNNNATC	1 cut(s)	910
BseLI	CCNNNNNNNGG	7 cut(s)	28, 1078, 1216, 1303, 1543, 1691, 2144
BseMI	GCAATG	2 cut(s)	1158, 1363
BseMII	CTCAG	5 cut(s)	693, 1075, 1275, 1698, 2356
BseNI	ACTGG	5 cut(s)	182, 946, 1127, 1186, 2023
BseSI	GKGCMC	1 cut(s)	1596
BseXI	GCAGC	4 cut(s)	1064, 1138, 1160, 1340
BseYI	CCCAGC	1 cut(s)	2206
BsgI	GTGCAG	1 cut(s)	2118
BshFI	GGCC	4 cut(s)	131, 953, 1126, 1658
BshVI	ATCGAT	1 cut(s)	876
BsiHKAI	GWGCWC	4 cut(s)	1596, 1620, 1977, 2152
BsiHKCI	CYCGRG	2 cut(s)	545, 2153
BsiSI	CCGG	1 cut(s)	132
BslFI	GGGAC	3 cut(s)	242, 250, 958
BslI	CCNNNNNNNGG	7 cut(s)	28, 1078, 1216, 1303, 1543, 1691, 2144
BsmAI	GTCTC	5 cut(s)	29, 246, 318, 2134, 2207
BsmBI	CGTCTC	1 cut(s)	246
BsmFI	GGGAC	3 cut(s)	242, 250, 958
BsmI	GAATGC	2 cut(s)	854, 2085
BsnI	GGCC	4 cut(s)	131, 953, 1126, 1658
BsoBI	CYCGRG	2 cut(s)	545, 2153
Bsp1286I	GDGCHC	4 cut(s)	1596, 1620, 1977, 2152
Bsp1407I	TGTACA	1 cut(s)	1512
Bsp143I	GATC	8 cut(s)	18, 86, 521, 663, 714, 964, 1287, 1791
BspACI	CCGC	6 cut(s)	5, 245, 660, 1142, 1505, 1530
BspANI	GGCC	4 cut(s)	131, 953, 1126, 1658
BspCNI	CTCAG	5 cut(s)	692, 1074, 1276, 1699, 2355
BspDI	ATCGAT	1 cut(s)	876
BspHI	TCATGA	2 cut(s)	1984, 2316
BspLI	GGNNCC	2 cut(s)	1854, 1917
BspMAI	CTGCAG	1 cut(s)	1330
BspMI	ACCTGC	1 cut(s)	1305
BspOI	GCTAGC	1 cut(s)	264
BspPI	GGATC	4 cut(s)	13, 94, 516, 671
BsrBI	CCGCTC	2 cut(s)	247, 660
BsrDI	GCAATG	2 cut(s)	1158, 1363
BsrGI	TGTACA	1 cut(s)	1512
BsrI	ACTGG	5 cut(s)	182, 946, 1127, 1186, 2023
BssECI	CCNNGG	6 cut(s)	916, 1072, 1073, 1341, 2154, 2327
BssMI	GATC	8 cut(s)	18, 86, 521, 663, 714, 964, 1287, 1791
BssNAI	GTATAC	1 cut(s)	1276
BssNI	GRCGYC	1 cut(s)	239
BssT1I	CCWWGG	1 cut(s)	916
Bst1107I	GTATAC	1 cut(s)	1276
Bst2UI	CCWGG	3 cut(s)	782, 1074, 1686
Bst4CI	ACNGT	8 cut(s)	411, 504, 723, 1679, 1761, 1848, 2025, 2279
Bst6I	CTCTTC	1 cut(s)	130
BstACI	GRCGYC	1 cut(s)	239
BstAPI	GCANNNNNTGC	2 cut(s)	1591, 2170
BstAUI	TGTACA	1 cut(s)	1512
BstBAI	YACGTR	1 cut(s)	851
BstC8I	GCNNGC	3 cut(s)	262, 1050, 1165
BstDEI	CTNAG	6 cut(s)	679, 1061, 1211, 1284, 1707, 2342
BstDSI	CCRYGG	2 cut(s)	1341, 2327
BstEII	GGTNACC	1 cut(s)	1532
BstENI	CCTNNNNNAGG	1 cut(s)	26
BstF5I	GGATG	3 cut(s)	570, 911, 1764
BstH2I	RGCGCY	1 cut(s)	338
BstHHI	GCGC	3 cut(s)	105, 337, 2085
BstKTI	GATC	8 cut(s)	21, 89, 524, 666, 717, 967, 1290, 1794
BstMAI	GTCTC	5 cut(s)	29, 246, 318, 2134, 2207
BstMBI	GATC	8 cut(s)	18, 86, 521, 663, 714, 964, 1287, 1791
BstMWI	GCNNNNNNNGC	7 cut(s)	959, 1148, 1160, 1169, 1591, 2170, 2308
BstNI	CCWGG	3 cut(s)	782, 1074, 1686
BstNSI	RCATGY	3 cut(s)	434, 1404, 1612
BstPI	GGTNACC	1 cut(s)	1532
BstSCI	CCNGG	3 cut(s)	780, 1072, 1684
BstSFI	CTRYAG	3 cut(s)	702, 1326, 2275
BstSLI	GKGCMC	1 cut(s)	1596
BstV1I	GCAGC	4 cut(s)	1064, 1138, 1160, 1340
BstV2I	GAAGAC	2 cut(s)	352, 474
BstZ17I	GTATAC	1 cut(s)	1276
Bsu15I	ATCGAT	1 cut(s)	876
BsuI	GTATCC	1 cut(s)	922
BsuRI	GGCC	4 cut(s)	131, 953, 1126, 1658
BsuTUI	ATCGAT	1 cut(s)	876
BtgI	CCRYGG	2 cut(s)	1341, 2327
BtsCI	GGATG	3 cut(s)	570, 911, 1764
BtsI	GCAGTG	2 cut(s)	1323, 1380
BtsIMutI	CAGTG	7 cut(s)	270, 288, 407, 744, 985, 1323, 1380
BveI	ACCTGC	1 cut(s)	1305
Cac8I	GCNNGC	3 cut(s)	262, 1050, 1165
CaiI	CAGNNNCTG	2 cut(s)	70, 686
CciI	TCATGA	2 cut(s)	1984, 2316
CfoI	GCGC	3 cut(s)	105, 337, 2085
ClaI	ATCGAT	1 cut(s)	876
Csp6I	GTAC	3 cut(s)	341, 1120, 1513
CviQI	GTAC	3 cut(s)	341, 1120, 1513
DdeI	CTNAG	6 cut(s)	679, 1061, 1211, 1284, 1707, 2342
DpnI	GATC	8 cut(s)	20, 88, 523, 665, 716, 966, 1289, 1793
DpnII	GATC	8 cut(s)	18, 86, 521, 663, 714, 964, 1287, 1791
EaeI	YGGCCR	1 cut(s)	1124
Eam1104I	CTCTTC	1 cut(s)	130
EarI	CTCTTC	1 cut(s)	130
EciI	GGCGGA	1 cut(s)	20
Ecl136II	GAGCTC	2 cut(s)	1618, 2150
Eco130I	CCWWGG	1 cut(s)	916
Eco24I	GRGCYC	2 cut(s)	1620, 2152
Eco47III	AGCGCT	1 cut(s)	336
Eco53kI	GAGCTC	2 cut(s)	1618, 2150
Eco57I	CTGAAG	3 cut(s)	92, 961, 2058
Eco88I	CYCGRG	2 cut(s)	545, 2153
Eco91I	GGTNACC	1 cut(s)	1532
EcoICRI	GAGCTC	2 cut(s)	1618, 2150
EcoNI	CCTNNNNNAGG	1 cut(s)	26
EcoO65I	GGTNACC	1 cut(s)	1532
EcoRII	CCWGG	3 cut(s)	780, 1072, 1684
EcoT14I	CCWWGG	1 cut(s)	916
EcoT22I	ATGCAT	1 cut(s)	1501
EcoT38I	GRGCYC	2 cut(s)	1620, 2152
ErhI	CCWWGG	1 cut(s)	916
Esp3I	CGTCTC	1 cut(s)	246
FalI	AAGNNNNNCTT	2 cut(s)	428, 460
FaqI	GGGAC	3 cut(s)	242, 250, 958
FauI	CCCGC	1 cut(s)	653
FauNDI	CATATG	1 cut(s)	1445
FblI	GTMKAC	3 cut(s)	111, 1275, 2274
Fnu4HI	GCNGC	4 cut(s)	1053, 1149, 1152, 1329
FokI	GGATG	3 cut(s)	557, 918, 1751
FriOI	GRGCYC	2 cut(s)	1620, 2152
Fsp4HI	GCNGC	4 cut(s)	1053, 1149, 1152, 1329
FspBI	CTAG	9 cut(s)	261, 674, 1424, 1440, 1635, 1887, 1940, 2106, 2334
GlaI	GCGC	3 cut(s)	104, 336, 2084
GluI	GCNGC	4 cut(s)	1053, 1149, 1152, 1329
GsaI	CCCAGC	1 cut(s)	2210
GsuI	CTGGAG	1 cut(s)	1203
HaeII	RGCGCY	1 cut(s)	338
HaeIII	GGCC	4 cut(s)	131, 953, 1126, 1658
HapII	CCGG	1 cut(s)	132
HhaI	GCGC	3 cut(s)	105, 337, 2085
Hin1I	GRCGYC	1 cut(s)	239
Hin6I	GCGC	3 cut(s)	103, 335, 2083
HinP1I	GCGC	3 cut(s)	103, 335, 2083
HincII	GTYRAC	5 cut(s)	112, 1750, 2059, 2112, 2266
HindII	GTYRAC	5 cut(s)	112, 1750, 2059, 2112, 2266
HinfI	GANTC	6 cut(s)	113, 759, 1100, 1723, 2042, 2322
HpaI	GTTAAC	2 cut(s)	1750, 2059
HpaII	CCGG	1 cut(s)	132
HphI	GGTGA	6 cut(s)	28, 401, 451, 577, 1402, 1519
Hpy166II	GTNNAC	8 cut(s)	112, 1276, 1594, 1750, 2059, 2112, 2266, 2275
Hpy188I	TCNGA	6 cut(s)	207, 682, 812, 1064, 1663, 1708
Hpy8I	GTNNAC	8 cut(s)	112, 1276, 1594, 1750, 2059, 2112, 2266, 2275
HpyAV	CCTTC	5 cut(s)	67, 147, 1072, 1547, 1982
HpyCH4III	ACNGT	8 cut(s)	411, 504, 723, 1679, 1761, 1848, 2025, 2279
HpyCH4IV	ACGT	3 cut(s)	239, 850, 1130
HpyF10VI	GCNNNNNNNGC	7 cut(s)	959, 1148, 1160, 1169, 1591, 2170, 2308
HpyF3I	CTNAG	6 cut(s)	679, 1061, 1211, 1284, 1707, 2342
HpySE526I	ACGT	3 cut(s)	239, 850, 1130
Hsp92I	GRCGYC	1 cut(s)	239
HspAI	GCGC	3 cut(s)	103, 335, 2083
KspAI	GTTAAC	2 cut(s)	1750, 2059
Kzo9I	GATC	8 cut(s)	18, 86, 521, 663, 714, 964, 1287, 1791
LmnI	GCTCC	6 cut(s)	475, 1184, 1852, 2147, 2155, 2308
Lsp1109I	GCAGC	4 cut(s)	1064, 1138, 1160, 1340
LweI	GCATC	4 cut(s)	673, 816, 1020, 1508
MaeI	CTAG	9 cut(s)	261, 674, 1424, 1440, 1635, 1887, 1940, 2106, 2334
MaeII	ACGT	3 cut(s)	239, 850, 1130
MaeIII	GTNAC	7 cut(s)	583, 669, 937, 971, 1321, 1532, 1895
MalI	GATC	8 cut(s)	20, 88, 523, 665, 716, 966, 1289, 1793
MbiI	CCGCTC	2 cut(s)	247, 660
MboI	GATC	8 cut(s)	18, 86, 521, 663, 714, 964, 1287, 1791
MboII	GAAGA	7 cut(s)	53, 147, 352, 479, 550, 900, 2051
MfeI	CAATTG	1 cut(s)	1652
MhlI	GDGCHC	4 cut(s)	1596, 1620, 1977, 2152
MlsI	TGGCCA	1 cut(s)	1126
MluNI	TGGCCA	1 cut(s)	1126
MlyI	GAGTC	2 cut(s)	107, 1094
MmeI	TCCRAC	4 cut(s)	531, 736, 1830, 2329
Mox20I	TGGCCA	1 cut(s)	1126
Mph1103I	ATGCAT	1 cut(s)	1501
MroXI	GAANNNNTTC	3 cut(s)	1091, 1833, 2046
MscI	TGGCCA	1 cut(s)	1126
MseI	TTAA	8 cut(s)	351, 446, 515, 1028, 1236, 1335, 1749, 2058
MslI	CAYNNNNRTG	2 cut(s)	590, 978
Msp20I	TGGCCA	1 cut(s)	1126
MspA1I	CMGCKG	1 cut(s)	1151
MspI	CCGG	1 cut(s)	132
MspR9I	CCNGG	3 cut(s)	782, 1074, 1686
MunI	CAATTG	1 cut(s)	1652
Mva1269I	GAATGC	2 cut(s)	854, 2085
MvaI	CCWGG	3 cut(s)	782, 1074, 1686
MwoI	GCNNNNNNNGC	7 cut(s)	959, 1148, 1160, 1169, 1591, 2170, 2308
NdeI	CATATG	1 cut(s)	1445
NdeII	GATC	8 cut(s)	18, 86, 521, 663, 714, 964, 1287, 1791
NheI	GCTAGC	1 cut(s)	260
NlaIV	GGNNCC	2 cut(s)	1854, 1917
NmuCI	GTSAC	4 cut(s)	583, 669, 971, 1321
NsiI	ATGCAT	1 cut(s)	1501
NspI	RCATGY	3 cut(s)	434, 1404, 1612
OliI	CACNNNNGTG	1 cut(s)	978
PaeR7I	CTCGAG	1 cut(s)	545
PagI	TCATGA	2 cut(s)	1984, 2316
PasI	CCCWGGG	1 cut(s)	1073
PciI	ACATGT	2 cut(s)	1400, 1608
PctI	GAATGC	2 cut(s)	854, 2085
PdmI	GAANNNNTTC	3 cut(s)	1091, 1833, 2046
PfeI	GAWTC	4 cut(s)	759, 1723, 2042, 2322
PflMI	CCANNNNNTGG	1 cut(s)	1691
PkrI	GCNGC	4 cut(s)	1054, 1150, 1153, 1330
PleI	GAGTC	2 cut(s)	107, 1094
PpsI	GAGTC	2 cut(s)	107, 1094
Ppu21I	YACGTR	1 cut(s)	851
PscI	ACATGT	2 cut(s)	1400, 1608
PshBI	ATTAAT	1 cut(s)	1028
Psp124BI	GAGCTC	2 cut(s)	1620, 2152
Psp1406I	AACGTT	1 cut(s)	1130
Psp6I	CCWGG	3 cut(s)	780, 1072, 1684
PspEI	GGTNACC	1 cut(s)	1532
PspFI	CCCAGC	1 cut(s)	2206
PspGI	CCWGG	3 cut(s)	780, 1072, 1684
PspN4I	GGNNCC	2 cut(s)	1854, 1917
PstI	CTGCAG	1 cut(s)	1330
PstNI	CAGNNNCTG	2 cut(s)	70, 686
PvuII	CAGCTG	1 cut(s)	1151
RsaI	GTAC	3 cut(s)	342, 1121, 1514
RsaNI	GTAC	3 cut(s)	341, 1120, 1513
RseI	CAYNNNNRTG	2 cut(s)	590, 978
SacI	GAGCTC	2 cut(s)	1620, 2152
SalI	GTCGAC	1 cut(s)	110
SaqAI	TTAA	8 cut(s)	351, 446, 515, 1028, 1236, 1335, 1749, 2058
SatI	GCNGC	4 cut(s)	1053, 1149, 1152, 1329
Sau3AI	GATC	8 cut(s)	18, 86, 521, 663, 714, 964, 1287, 1791
SchI	GAGTC	2 cut(s)	107, 1094
ScrFI	CCNGG	3 cut(s)	782, 1074, 1686
SduI	GDGCHC	4 cut(s)	1596, 1620, 1977, 2152
SfaNI	GCATC	4 cut(s)	673, 816, 1020, 1508
SfcI	CTRYAG	3 cut(s)	702, 1326, 2275
Sfr274I	CTCGAG	1 cut(s)	545
SlaI	CTCGAG	1 cut(s)	545
SmiMI	CAYNNNNRTG	2 cut(s)	590, 978
SmlI	CTYRAG	3 cut(s)	545, 1619, 2284
SmoI	CTYRAG	3 cut(s)	545, 1619, 2284
SpeI	ACTAGT	1 cut(s)	1939
SsiI	CCGC	6 cut(s)	5, 245, 660, 1142, 1505, 1530
SspI	AATATT	1 cut(s)	927
SspMI	CTAG	9 cut(s)	261, 674, 1424, 1440, 1635, 1887, 1940, 2106, 2334
SstI	GAGCTC	2 cut(s)	1620, 2152
StyD4I	CCNGG	3 cut(s)	780, 1072, 1684
StyI	CCWWGG	1 cut(s)	916
TaaI	ACNGT	8 cut(s)	411, 504, 723, 1679, 1761, 1848, 2025, 2279
TaiI	ACGT	3 cut(s)	242, 853, 1133
TaqI	TCGA	8 cut(s)	111, 546, 876, 1485, 1614, 1790, 1843, 2122
TatI	WGTACW	2 cut(s)	1119, 1512
TfiI	GAWTC	4 cut(s)	759, 1723, 2042, 2322
Tru1I	TTAA	8 cut(s)	351, 446, 515, 1028, 1236, 1335, 1749, 2058
Tru9I	TTAA	8 cut(s)	351, 446, 515, 1028, 1236, 1335, 1749, 2058
TscAI	CASTG	7 cut(s)	277, 295, 414, 751, 985, 1330, 1380
TseFI	GTSAC	4 cut(s)	583, 669, 971, 1321
TseI	GCWGC	4 cut(s)	1052, 1148, 1151, 1328
Tsp45I	GTSAC	4 cut(s)	583, 669, 971, 1321
TspGWI	ACGGA	4 cut(s)	962, 1358, 1865, 2344
TspRI	CASTG	7 cut(s)	277, 295, 414, 751, 985, 1330, 1380
Van91I	CCANNNNNTGG	1 cut(s)	1691
VneI	GTGCAC	1 cut(s)	1592
VspI	ATTAAT	1 cut(s)	1028
XagI	CCTNNNNNAGG	1 cut(s)	26
XapI	RAATTY	3 cut(s)	1517, 1948, 1958
XceI	RCATGY	3 cut(s)	434, 1404, 1612
XhoI	CTCGAG	1 cut(s)	545
XmiI	GTMKAC	3 cut(s)	111, 1275, 2274
XmnI	GAANNNNTTC	3 cut(s)	1091, 1833, 2046
XspI	CTAG	9 cut(s)	261, 674, 1424, 1440, 1635, 1887, 1940, 2106, 2334
ZraI	GACGTC	1 cut(s)	240
Zsp2I	ATGCAT	1 cut(s)	1501

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.