Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004734

Physical Location & Seq

Genomic Coordinates Forward (+)

10200806 .. 10206915

Gene Structure 6110 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1133200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2370 bp

ATGGCGGAGAAAGTGGTGAGCTTGAAAGAGGAAGAGACAGAAGAAGCAAAAGAAATAACGAACCAGCAACTGAAGGAGAGAAAAGTGTCATGGGCAAAGTTGCGCCGTGTCGACTCTCTCAATTTGGAGGCCGGAAGGGTTTCCATGAATGGAAGGCATGGCTCACAGGTAAATAACTGGCATAGAACGTTGAGTTTGGCATTTCAGAGCATTGGGATTGTGTATGGGGACATAGGAACGTCGCCGCTCTATGTGTATGAGAGCACTTTCACTGATGGTATTGACAACAATGACGACATTATAGGGGTACTATCTCTCATTATCTATACCATAGCGCTCCTGCCTCTACTTAAGTATGTCTTCATCGTGCTATGGGCTAATGACAACGGTGATGGAGGGACATTTGCATTGTATTCCTTGATGTGCCGACATGCAAAGGTGAGCTTAACTCCAAATAACCAACCAGAAGACAGGGAGCTATCAAACTACAAACTTGAAACACCATCCAACGAGTTAAAACGATCCCAAACCATCAAGAAGAAGCTCGAGAATAGTAAAATTGCCAAATACAGCCTTTTCCTTGTCACCATTATGGGAACTTCAATGGTTATTGGAGATGGGGTTCTTACTCCTTGCATTTCAGTCCTTTCTGCTGTGAGCGGGATCAAATCATTAGGCACAGATACTGTCGTGGGGATTTCTATAGTAATCTTGATCATACTCTTTTCTGCTCAAAGATTTGGCACTGACAAAGTGGGATTCACCTTCGCACCTATTATCCTTGTGTGGTTCTTGTTCATCGGTGGCATCGGACTTTACAACTTATTCAAACATGACGTTGGTGTATTACGTGCTTTCAATCCAAAATATATCATCGATTTCTTTAGACGAAATGGGAAAAGGGGATGGATTTCCCTTGGGGGAATATTCCTCTGCATTACTGGGACCGAGGCCATGTTTGCTGATCTGGGTCACTTCACCGTCAAGGCTATCCAAGTGAGTTTCTCTTGCATCACATTTCCTGCATTAGTACTTGCATATAGTGGGCAAGCAGCGTTCCTCAGAAAGTTCCCAGGGAAGGTGGACCAGACATTCTATGACTCAATACCAGACCCTTTGTACTGGCCAACGTTCGTTGTGGCGGTAGCAGCTGCCATCATTGCCAGCCAAGCTATGATATCTGGAGCATTTTCAATTATCTCTCAATCCCTGAGTTTGGGTTGTTTTCCAAGAGTTAAGGTTGTTCATACCTCTGCTAAGTATGAGGGCCAAGTATACATACCTGAGATCAACTACATACTCATGGTTGCTTGTGTAATTGTCACTGCAGCCTTTAAGACCACAGAAAAAATTGGTAATGCATATGGAATTGCAGTGGTCAGTGTAATGGTGATCACAACATGTTTGCTTACTCTAATAATGTTGGTGATATGGAAGACTAGCATATGGTTGATTGGTCTGTTCTTTGTGGTGTTCTTTTGTGTCGAGGTGGTTTATGCATCTGCGGTGATGTACAAATTCGTTCAAGGCGGTTTCCTTCCTCTGGTCTTTTCTTTTTTCCTTATGGTAATCATGGGGATTTGGCATTATGTGCACCATCAAAGATACATGTTCGAGCTCAAGAATAAGGTTTCTAGTGAATACATGAAACAATTGGCCTCTAACCCCAACATAAACCGTGTGCCAGGAATTGGACTTCTATATTCTGAGCTTGTGCAAGGTATTCCCCCTATATTTTCTCACTTTGTTAACAACATACCGTCCATCCACTCTGTTATAGTGATTGTGTCGGTCAAGCCTATTCCATTCAGCAAAGTCGCATTGGAGGAAAGGTTTCTATTTCGACAGTTGGAGCCAAGAGATTACCGAATATTCCGTTGTGTCGCCAGGTATGGTTATAATGATAGAATGGGGGAACCCAAAGAGTTTGAGGAACAACTAGTTGAGCATTTGAAAGAATTTATCCGCCATGAGCACTTCGCACATGAAGGAGAAATAACAAACGAGGCTGTTTCAACTGGACAGTCATCTAACGCGGAAGAATCAGCTGAACAAGTTAACCAACCTCGTGGTTCATCAGGGCGCATTCTATCATTTAATACTGCCGGATCAACATCTAACGGAATAGTCTCTGCACCCGTCAAAGGAGCTGTGGAGGAAATGCAGTTCGTTCAAGAGGCTATGGAGAAAGGCATAGTTTATCTGCTGGGACAGACGGAAGTGGTGGCAGAAGCAAACTCGTCATTGTTCAAGAGGATAGTGGTCAATCATGTCTACAGTTTCTTGAGGAAAAACTTTAGGCAAGGGGAGCAAGTCATGAGAATCCCACGGAGTAGGCTTCTCAGGGTTGGAATGACCTATGAAATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0008150 GO:0008324 GO:0009674 GO:0009987 GO:0010107 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015291 GO:0015293 GO:0015294 GO:0015318 GO:0015370 GO:0015672 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0035725 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

790

Amino Acids

88.28

Weight (kDa)

8.76

Isoelectric Point (pI)

27.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	66 - 548	4.9e-167	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	562 - 789	3.2e-26	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004734)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13420
fragaria_vesca	FvH4_4g22240 FvH4_4g22240 FvH4_4g22260
malus_domestica	MD13G1133100.v1.1 MD13G1133200.v1.1 MD16G1143900.v1.1
prunus_persica	Prupe.1G212100_v2.0.a1 Prupe.1G212200_v2.0.a1
pyrus_communis	pycom13g11630
rosa_chinensis	RchiOBHm_Chr4g0428481 RchiOBHm_Chr4g0428501
rosa_laevigata	RLG00000007145 RLG00000007146
rosa_multiflora	Rmu_sc0002072.1_g000037 Rmu_sc0004623.1_g000012 Rmu_sc0006844.1_g000008
rosa_roxburghii	Rroxscaffold_5G00370180 Rroxscaffold_5G00370210
rosa_rugosa	Rorug04G0225500 Rorug04G0225600
rosa_samantha	Rh4AG283100 Rh4AG283200 Rh4BG288800 Rh4BG288900 Rh4CG304400 Rh4CG304900 Rh4DG285900 Rh4DG286000
rosa_wichuraiana	Rw4G024580 Rw4G024600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1899
AccB7I	CCANNNNNTGG	1 cut(s)	1691
AccBSI	CCGCTC	2 cut(s)	247, 660
AccI	GTMKAC	3 cut(s)	111, 1275, 2274
AccII	CGCG	1 cut(s)	2036
AciI	CCGC	8 cut(s)	5, 245, 660, 1142, 1505, 1530, 1966, 2036
AclI	AACGTT	2 cut(s)	188, 1130
AclWI	GGATC	3 cut(s)	516, 671, 2116
AcoI	YGGCCR	1 cut(s)	1124
AcsI	RAATTY	2 cut(s)	1517, 1958
AcuI	CTGAAG	1 cut(s)	92
AfaI	GTAC	4 cut(s)	309, 1032, 1121, 1514
AfeI	AGCGCT	1 cut(s)	336
AfiI	CCNNNNNNNGG	5 cut(s)	1078, 1216, 1543, 1691, 2144
AflII	CTTAAG	1 cut(s)	350
AflIII	ACRYGT	2 cut(s)	1400, 1608
AhlI	ACTAGT	1 cut(s)	1939
AjnI	CCWGG	3 cut(s)	1072, 1684, 1886
AloI	GAACNNNNNNTCC	4 cut(s)	606, 638, 2150, 2182
Alw21I	GWGCWC	4 cut(s)	266, 1596, 1620, 1977
Alw26I	GTCTC	2 cut(s)	29, 2134
Alw44I	GTGCAC	1 cut(s)	1592
AlwI	GGATC	3 cut(s)	516, 671, 2116
AlwNI	CAGNNNCTG	2 cut(s)	70, 686
Ama87I	CYCGRG	1 cut(s)	545
Aor51HI	AGCGCT	1 cut(s)	336
AoxI	GGCC	5 cut(s)	129, 951, 1124, 1267, 1656
ApaLI	GTGCAC	1 cut(s)	1592
ApeKI	GCWGC	4 cut(s)	1052, 1148, 1151, 1328
ApoI	RAATTY	2 cut(s)	1517, 1958
Asp700I	GAANNNNTTC	2 cut(s)	139, 1833
AspLEI	GCGC	3 cut(s)	105, 337, 2085
AspS9I	GGNCC	3 cut(s)	945, 1084, 1267
AsuHPI	GGTGA	9 cut(s)	28, 401, 451, 577, 754, 970, 1402, 1438, 1519
AvaI	CYCGRG	1 cut(s)	545
AvaII	GGWCC	2 cut(s)	945, 1084
BaeGI	GKGCMC	1 cut(s)	1596
BaeI	ACNNNNGTAYC	2 cut(s)	675, 708
BalI	TGGCCA	1 cut(s)	1126
BanII	GRGCYC	1 cut(s)	1620
BauI	CACGAG	1 cut(s)	2067
BbsI	GAAGAC	3 cut(s)	352, 474, 1442
Bbv12I	GWGCWC	4 cut(s)	266, 1596, 1620, 1977
BbvI	GCAGC	4 cut(s)	1064, 1138, 1160, 1340
BccI	CCATC	9 cut(s)	269, 386, 511, 539, 611, 900, 1163, 1605, 1772
BceAI	ACGGC	1 cut(s)	90
BciT130I	CCWGG	3 cut(s)	1074, 1686, 1888
BclI	TGATCA	2 cut(s)	714, 1392
BcoDI	GTCTC	2 cut(s)	29, 2134
BcuI	ACTAGT	1 cut(s)	1939
BfaI	CTAG	3 cut(s)	1440, 1635, 1940
BfmI	CTRYAG	3 cut(s)	702, 1326, 2275
BfoI	RGCGCY	1 cut(s)	338
BfrI	CTTAAG	1 cut(s)	350
BisI	GCNGC	5 cut(s)	245, 1053, 1149, 1152, 1329
BlsI	GCNGC	5 cut(s)	246, 1054, 1150, 1153, 1330
BmcAI	AGTACT	1 cut(s)	1032
Bme1390I	CCNGG	3 cut(s)	1074, 1686, 1888
Bme18I	GGWCC	2 cut(s)	945, 1084
BmeT110I	CYCGRG	1 cut(s)	545
BmgT120I	GGNCC	3 cut(s)	945, 1084, 1267
BmiI	GGNNCC	3 cut(s)	946, 1854, 1917
BmrFI	CCNGG	3 cut(s)	1074, 1686, 1888
BmrI	ACTGGG	1 cut(s)	951
BmsI	GCATC	3 cut(s)	816, 1020, 1508
BmuI	ACTGGG	1 cut(s)	951
BpiI	GAAGAC	3 cut(s)	352, 474, 1442
BplI	GAGNNNNNCTC	2 cut(s)	433, 465
BpmI	CTGGAG	1 cut(s)	1203
BpuEI	CTTGAG	2 cut(s)	1604, 2305
Bsa29I	ATCGAT	1 cut(s)	876
BsaAI	YACGTR	1 cut(s)	851
BsaBI	GATNNNNATC	1 cut(s)	2113
BsaJI	CCNNGG	5 cut(s)	916, 948, 1072, 1073, 2327
BsaXI	ACNNNNNCTCC	4 cut(s)	606, 636, 1817, 1847
Bsc4I	CCNNNNNNNGG	5 cut(s)	1078, 1216, 1543, 1691, 2144
Bse1I	ACTGG	4 cut(s)	182, 946, 1127, 2023
Bse3DI	GCAATG	1 cut(s)	1158
Bse8I	GATNNNNATC	1 cut(s)	2113
BseBI	CCWGG	3 cut(s)	1074, 1686, 1888
BseCI	ATCGAT	1 cut(s)	876
BseDI	CCNNGG	5 cut(s)	916, 948, 1072, 1073, 2327
BseGI	GGATG	3 cut(s)	503, 911, 1764
BseJI	GATNNNNATC	1 cut(s)	2113
BseLI	CCNNNNNNNGG	5 cut(s)	1078, 1216, 1543, 1691, 2144
BseMI	GCAATG	1 cut(s)	1158
BseMII	CTCAG	5 cut(s)	1075, 1202, 1275, 1698, 2356
BseNI	ACTGG	4 cut(s)	182, 946, 1127, 2023
BseSI	GKGCMC	1 cut(s)	1596
BseXI	GCAGC	4 cut(s)	1064, 1138, 1160, 1340
BseYI	CCCAGC	1 cut(s)	2206
BsgI	GTGCAG	1 cut(s)	2118
Bsh1236I	CGCG	1 cut(s)	2036
BshFI	GGCC	5 cut(s)	131, 953, 1126, 1269, 1658
BshVI	ATCGAT	1 cut(s)	876
BsiHKAI	GWGCWC	4 cut(s)	266, 1596, 1620, 1977
BsiHKCI	CYCGRG	1 cut(s)	545
BsiSI	CCGG	2 cut(s)	132, 2106
BslFI	GGGAC	4 cut(s)	242, 412, 958, 2223
BslI	CCNNNNNNNGG	5 cut(s)	1078, 1216, 1543, 1691, 2144
BsmAI	GTCTC	2 cut(s)	29, 2134
BsmFI	GGGAC	4 cut(s)	242, 412, 958, 2223
BsmI	GAATGC	1 cut(s)	2085
BsnI	GGCC	5 cut(s)	131, 953, 1126, 1269, 1658
BsoBI	CYCGRG	1 cut(s)	545
Bsp1286I	GDGCHC	4 cut(s)	266, 1596, 1620, 1977
Bsp1407I	TGTACA	1 cut(s)	1512
Bsp143I	GATC	7 cut(s)	521, 663, 714, 964, 1287, 1392, 2108
BspACI	CCGC	8 cut(s)	5, 245, 660, 1142, 1505, 1530, 1966, 2036
BspANI	GGCC	5 cut(s)	131, 953, 1126, 1269, 1658
BspCNI	CTCAG	5 cut(s)	1074, 1203, 1276, 1699, 2355
BspDI	ATCGAT	1 cut(s)	876
BspFNI	CGCG	1 cut(s)	2036
BspHI	TCATGA	1 cut(s)	2316
BspLI	GGNNCC	3 cut(s)	946, 1854, 1917
BspMAI	CTGCAG	1 cut(s)	1330
BspPI	GGATC	3 cut(s)	516, 671, 2116
BspTI	CTTAAG	1 cut(s)	350
BsrBI	CCGCTC	2 cut(s)	247, 660
BsrDI	GCAATG	1 cut(s)	1158
BsrGI	TGTACA	1 cut(s)	1512
BsrI	ACTGG	4 cut(s)	182, 946, 1127, 2023
BssECI	CCNNGG	5 cut(s)	916, 948, 1072, 1073, 2327
BssMI	GATC	7 cut(s)	521, 663, 714, 964, 1287, 1392, 2108
BssNAI	GTATAC	1 cut(s)	1276
BssSI	CACGAG	1 cut(s)	2067
BssT1I	CCWWGG	1 cut(s)	916
Bst1107I	GTATAC	1 cut(s)	1276
Bst2BI	CACGAG	1 cut(s)	2067
Bst2UI	CCWGG	3 cut(s)	1074, 1686, 1888
Bst4CI	ACNGT	8 cut(s)	389, 688, 982, 1679, 1761, 1848, 2025, 2279
Bst6I	CTCTTC	1 cut(s)	27
BstAFI	CTTAAG	1 cut(s)	350
BstAPI	GCANNNNNTGC	1 cut(s)	1591
BstAUI	TGTACA	1 cut(s)	1512
BstBAI	YACGTR	1 cut(s)	851
BstC8I	GCNNGC	2 cut(s)	1050, 1165
BstDEI	CTNAG	6 cut(s)	1061, 1211, 1257, 1284, 1707, 2342
BstDSI	CCRYGG	1 cut(s)	2327
BstF5I	GGATG	3 cut(s)	503, 911, 1764
BstFNI	CGCG	1 cut(s)	2036
BstH2I	RGCGCY	1 cut(s)	338
BstHHI	GCGC	3 cut(s)	105, 337, 2085
BstKTI	GATC	7 cut(s)	524, 666, 717, 967, 1290, 1395, 2111
BstMAI	GTCTC	2 cut(s)	29, 2134
BstMBI	GATC	7 cut(s)	521, 663, 714, 964, 1287, 1392, 2108
BstMWI	GCNNNNNNNGC	6 cut(s)	959, 1148, 1160, 1169, 1591, 2308
BstNI	CCWGG	3 cut(s)	1074, 1686, 1888
BstNSI	RCATGY	3 cut(s)	434, 1404, 1612
BstSCI	CCNGG	3 cut(s)	1072, 1684, 1886
BstSFI	CTRYAG	3 cut(s)	702, 1326, 2275
BstSLI	GKGCMC	1 cut(s)	1596
BstUI	CGCG	1 cut(s)	2036
BstV1I	GCAGC	4 cut(s)	1064, 1138, 1160, 1340
BstV2I	GAAGAC	3 cut(s)	352, 474, 1442
BstXI	CCANNNNNNTGG	1 cut(s)	2069
BstZ17I	GTATAC	1 cut(s)	1276
Bsu15I	ATCGAT	1 cut(s)	876
BsuRI	GGCC	5 cut(s)	131, 953, 1126, 1269, 1658
BsuTUI	ATCGAT	1 cut(s)	876
BtgI	CCRYGG	1 cut(s)	2327
BtsCI	GGATG	3 cut(s)	503, 911, 1764
BtsI	GCAGTG	2 cut(s)	1323, 1380
BtsIMutI	CAGTG	5 cut(s)	270, 744, 1323, 1380, 1387
Cac8I	GCNNGC	2 cut(s)	1050, 1165
CaiI	CAGNNNCTG	2 cut(s)	70, 686
CciI	TCATGA	1 cut(s)	2316
CfoI	GCGC	3 cut(s)	105, 337, 2085
Cfr13I	GGNCC	3 cut(s)	945, 1084, 1267
ClaI	ATCGAT	1 cut(s)	876
Csp6I	GTAC	4 cut(s)	308, 1031, 1120, 1513
CviQI	GTAC	4 cut(s)	308, 1031, 1120, 1513
DdeI	CTNAG	6 cut(s)	1061, 1211, 1257, 1284, 1707, 2342
DpnI	GATC	7 cut(s)	523, 665, 716, 966, 1289, 1394, 2110
DpnII	GATC	7 cut(s)	521, 663, 714, 964, 1287, 1392, 2108
EaeI	YGGCCR	1 cut(s)	1124
Eam1104I	CTCTTC	1 cut(s)	27
EarI	CTCTTC	1 cut(s)	27
EciI	GGCGGA	2 cut(s)	20, 1955
Ecl136II	GAGCTC	1 cut(s)	1618
Eco130I	CCWWGG	1 cut(s)	916
Eco24I	GRGCYC	1 cut(s)	1620
Eco32I	GATATC	1 cut(s)	1179
Eco47I	GGWCC	2 cut(s)	945, 1084
Eco47III	AGCGCT	1 cut(s)	336
Eco53kI	GAGCTC	1 cut(s)	1618
Eco57I	CTGAAG	1 cut(s)	92
Eco88I	CYCGRG	1 cut(s)	545
EcoICRI	GAGCTC	1 cut(s)	1618
EcoRII	CCWGG	3 cut(s)	1072, 1684, 1886
EcoRV	GATATC	1 cut(s)	1179
EcoT14I	CCWWGG	1 cut(s)	916
EcoT22I	ATGCAT	2 cut(s)	1363, 1501
EcoT38I	GRGCYC	1 cut(s)	1620
ErhI	CCWWGG	1 cut(s)	916
FalI	AAGNNNNNCTT	4 cut(s)	344, 376, 428, 460
FaqI	GGGAC	4 cut(s)	242, 412, 958, 2223
FauI	CCCGC	1 cut(s)	653
FauNDI	CATATG	2 cut(s)	1363, 1445
FbaI	TGATCA	2 cut(s)	714, 1392
FblI	GTMKAC	3 cut(s)	111, 1275, 2274
Fnu4HI	GCNGC	5 cut(s)	245, 1053, 1149, 1152, 1329
FokI	GGATG	3 cut(s)	490, 918, 1751
FriOI	GRGCYC	1 cut(s)	1620
Fsp4HI	GCNGC	5 cut(s)	245, 1053, 1149, 1152, 1329
FspBI	CTAG	3 cut(s)	1440, 1635, 1940
GlaI	GCGC	3 cut(s)	104, 336, 2084
GluI	GCNGC	5 cut(s)	245, 1053, 1149, 1152, 1329
GsaI	CCCAGC	1 cut(s)	2210
GsuI	CTGGAG	1 cut(s)	1203
HaeII	RGCGCY	1 cut(s)	338
HaeIII	GGCC	5 cut(s)	131, 953, 1126, 1269, 1658
HapII	CCGG	2 cut(s)	132, 2106
HhaI	GCGC	3 cut(s)	105, 337, 2085
Hin6I	GCGC	3 cut(s)	103, 335, 2083
HinP1I	GCGC	3 cut(s)	103, 335, 2083
HincII	GTYRAC	3 cut(s)	112, 1750, 2059
HindII	GTYRAC	3 cut(s)	112, 1750, 2059
HinfI	GANTC	5 cut(s)	113, 759, 1100, 2042, 2322
HpaI	GTTAAC	2 cut(s)	1750, 2059
HpaII	CCGG	2 cut(s)	132, 2106
HphI	GGTGA	9 cut(s)	28, 401, 451, 577, 754, 970, 1402, 1438, 1519
Hpy166II	GTNNAC	7 cut(s)	112, 1084, 1276, 1594, 1750, 2059, 2275
Hpy188I	TCNGA	4 cut(s)	207, 812, 1064, 1708
Hpy188III	TCNNGA	9 cut(s)	535, 547, 712, 1182, 1621, 2174, 2251, 2284, 2317
Hpy8I	GTNNAC	7 cut(s)	112, 1084, 1276, 1594, 1750, 2059, 2275
Hpy99I	CGWCG	1 cut(s)	244
HpyAV	CCTTC	7 cut(s)	67, 129, 147, 775, 1072, 1547, 1982
HpyCH4III	ACNGT	8 cut(s)	389, 688, 982, 1679, 1761, 1848, 2025, 2279
HpyCH4IV	ACGT	5 cut(s)	188, 239, 837, 850, 1130
HpyF10VI	GCNNNNNNNGC	6 cut(s)	959, 1148, 1160, 1169, 1591, 2308
HpyF3I	CTNAG	6 cut(s)	1061, 1211, 1257, 1284, 1707, 2342
HpySE526I	ACGT	5 cut(s)	188, 239, 837, 850, 1130
HspAI	GCGC	3 cut(s)	103, 335, 2083
Ksp22I	TGATCA	2 cut(s)	714, 1392
KspAI	GTTAAC	2 cut(s)	1750, 2059
Kzo9I	GATC	7 cut(s)	521, 663, 714, 964, 1287, 1392, 2108
LmnI	GCTCC	6 cut(s)	342, 475, 1184, 1852, 2147, 2308
Lsp1109I	GCAGC	4 cut(s)	1064, 1138, 1160, 1340
LweI	GCATC	3 cut(s)	816, 1020, 1508
MaeI	CTAG	3 cut(s)	1440, 1635, 1940
MaeII	ACGT	5 cut(s)	188, 239, 837, 850, 1130
MaeIII	GTNAC	3 cut(s)	583, 971, 1321
MalI	GATC	7 cut(s)	523, 665, 716, 966, 1289, 1394, 2110
MbiI	CCGCTC	2 cut(s)	247, 660
MboI	GATC	7 cut(s)	521, 663, 714, 964, 1287, 1392, 2108
MboII	GAAGA	7 cut(s)	44, 53, 352, 479, 550, 1447, 2051
MfeI	CAATTG	1 cut(s)	1652
MhlI	GDGCHC	4 cut(s)	266, 1596, 1620, 1977
MlsI	TGGCCA	1 cut(s)	1126
MluNI	TGGCCA	1 cut(s)	1126
MlyI	GAGTC	2 cut(s)	107, 1094
MmeI	TCCRAC	3 cut(s)	531, 1830, 2329
Mox20I	TGGCCA	1 cut(s)	1126
Mph1103I	ATGCAT	2 cut(s)	1363, 1501
MroXI	GAANNNNTTC	2 cut(s)	139, 1833
MscI	TGGCCA	1 cut(s)	1126
MseI	TTAA	8 cut(s)	351, 446, 515, 1236, 1335, 1749, 2058, 2097
MslI	CAYNNNNRTG	2 cut(s)	590, 1301
Msp20I	TGGCCA	1 cut(s)	1126
MspA1I	CMGCKG	2 cut(s)	1151, 2048
MspCI	CTTAAG	1 cut(s)	350
MspI	CCGG	2 cut(s)	132, 2106
MspR9I	CCNGG	3 cut(s)	1074, 1686, 1888
MunI	CAATTG	1 cut(s)	1652
Mva1269I	GAATGC	1 cut(s)	2085
MvaI	CCWGG	3 cut(s)	1074, 1686, 1888
MvnI	CGCG	1 cut(s)	2036
MwoI	GCNNNNNNNGC	6 cut(s)	959, 1148, 1160, 1169, 1591, 2308
NdeI	CATATG	2 cut(s)	1363, 1445
NdeII	GATC	7 cut(s)	521, 663, 714, 964, 1287, 1392, 2108
NlaIV	GGNNCC	3 cut(s)	946, 1854, 1917
NmuCI	GTSAC	3 cut(s)	583, 971, 1321
NsiI	ATGCAT	2 cut(s)	1363, 1501
NspI	RCATGY	3 cut(s)	434, 1404, 1612
PaeR7I	CTCGAG	1 cut(s)	545
PagI	TCATGA	1 cut(s)	2316
PasI	CCCWGGG	1 cut(s)	1073
PciI	ACATGT	2 cut(s)	1400, 1608
PctI	GAATGC	1 cut(s)	2085
PdmI	GAANNNNTTC	2 cut(s)	139, 1833
PfeI	GAWTC	3 cut(s)	759, 2042, 2322
PflMI	CCANNNNNTGG	1 cut(s)	1691
PkrI	GCNGC	5 cut(s)	246, 1054, 1150, 1153, 1330
PleI	GAGTC	2 cut(s)	107, 1094
PpsI	GAGTC	2 cut(s)	107, 1094
Ppu21I	YACGTR	1 cut(s)	851
PscI	ACATGT	2 cut(s)	1400, 1608
PsiI	TTATAA	1 cut(s)	1899
Psp124BI	GAGCTC	1 cut(s)	1620
Psp1406I	AACGTT	2 cut(s)	188, 1130
Psp6I	CCWGG	3 cut(s)	1072, 1684, 1886
PspFI	CCCAGC	1 cut(s)	2206
PspGI	CCWGG	3 cut(s)	1072, 1684, 1886
PspN4I	GGNNCC	3 cut(s)	946, 1854, 1917
PspPI	GGNCC	3 cut(s)	945, 1084, 1267
PstI	CTGCAG	1 cut(s)	1330
PstNI	CAGNNNCTG	2 cut(s)	70, 686
PvuII	CAGCTG	2 cut(s)	1151, 2048
RsaI	GTAC	4 cut(s)	309, 1032, 1121, 1514
RsaNI	GTAC	4 cut(s)	308, 1031, 1120, 1513
RseI	CAYNNNNRTG	2 cut(s)	590, 1301
SacI	GAGCTC	1 cut(s)	1620
SalI	GTCGAC	1 cut(s)	110
SaqAI	TTAA	8 cut(s)	351, 446, 515, 1236, 1335, 1749, 2058, 2097
SatI	GCNGC	5 cut(s)	245, 1053, 1149, 1152, 1329
Sau3AI	GATC	7 cut(s)	521, 663, 714, 964, 1287, 1392, 2108
Sau96I	GGNCC	3 cut(s)	945, 1084, 1267
ScaI	AGTACT	1 cut(s)	1032
SchI	GAGTC	2 cut(s)	107, 1094
ScrFI	CCNGG	3 cut(s)	1074, 1686, 1888
SduI	GDGCHC	4 cut(s)	266, 1596, 1620, 1977
SfaNI	GCATC	3 cut(s)	816, 1020, 1508
SfcI	CTRYAG	3 cut(s)	702, 1326, 2275
Sfr274I	CTCGAG	1 cut(s)	545
SinI	GGWCC	2 cut(s)	945, 1084
SlaI	CTCGAG	1 cut(s)	545
SmiMI	CAYNNNNRTG	2 cut(s)	590, 1301
SmlI	CTYRAG	4 cut(s)	350, 545, 1619, 2284
SmoI	CTYRAG	4 cut(s)	350, 545, 1619, 2284
SpeI	ACTAGT	1 cut(s)	1939
SsiI	CCGC	8 cut(s)	5, 245, 660, 1142, 1505, 1530, 1966, 2036
SspI	AATATT	2 cut(s)	927, 1872
SspMI	CTAG	3 cut(s)	1440, 1635, 1940
SstI	GAGCTC	1 cut(s)	1620
StyD4I	CCNGG	3 cut(s)	1072, 1684, 1886
StyI	CCWWGG	1 cut(s)	916
TaaI	ACNGT	8 cut(s)	389, 688, 982, 1679, 1761, 1848, 2025, 2279
TaiI	ACGT	5 cut(s)	191, 242, 840, 853, 1133
TaqI	TCGA	6 cut(s)	111, 546, 876, 1485, 1614, 1843
TaqII	GACCGA	2 cut(s)	962, 1780
TatI	WGTACW	3 cut(s)	1030, 1119, 1512
TauI	GCSGC	1 cut(s)	247
TfiI	GAWTC	3 cut(s)	759, 2042, 2322
Tru1I	TTAA	8 cut(s)	351, 446, 515, 1236, 1335, 1749, 2058, 2097
Tru9I	TTAA	8 cut(s)	351, 446, 515, 1236, 1335, 1749, 2058, 2097
TscAI	CASTG	5 cut(s)	277, 751, 1330, 1380, 1387
TseFI	GTSAC	3 cut(s)	583, 971, 1321
TseI	GCWGC	4 cut(s)	1052, 1148, 1151, 1328
Tsp45I	GTSAC	3 cut(s)	583, 971, 1321
TspDTI	ATGAA	7 cut(s)	161, 352, 787, 1235, 1661, 2001, 2064
TspGWI	ACGGA	4 cut(s)	1865, 2136, 2231, 2344
TspRI	CASTG	5 cut(s)	277, 751, 1330, 1380, 1387
Van91I	CCANNNNNTGG	1 cut(s)	1691
Vha464I	CTTAAG	1 cut(s)	350
VneI	GTGCAC	1 cut(s)	1592
VpaK11BI	GGWCC	2 cut(s)	945, 1084
XapI	RAATTY	2 cut(s)	1517, 1958
XceI	RCATGY	3 cut(s)	434, 1404, 1612
XhoI	CTCGAG	1 cut(s)	545
XmiI	GTMKAC	3 cut(s)	111, 1275, 2274
XmnI	GAANNNNTTC	2 cut(s)	139, 1833
XspI	CTAG	3 cut(s)	1440, 1635, 1940
ZrmI	AGTACT	1 cut(s)	1032
Zsp2I	ATGCAT	2 cut(s)	1363, 1501

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.