Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0004424

Physical Location & Seq

Genomic Coordinates Reverse (-)

25321670 .. 25324865

Gene Structure 3196 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g22520.t6

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1899 bp

ATGGGAAGCAGAGAAATCAAGTCCCCTGAAGGAGGGATAGAGACTCCAGCTAATTTGGAGGAACAATATTCAGACACAAATCCAAGGAAGAAGCTTGGTTTTTACTTCATCGAATCCGATGACAGGAGGACAGCTTTTGGAAGGGGATATACCGGAGGAACCACACCGGTTAATATCCATGGCAAACCAATCGCTGATCTTTCCAAAACCGGTGGTTGGTTTGCTGCCTTCTTCATATTTGGAAATGAAATGGCCGAGAGAATGGCTTATTTTGGACTCTCAGTGAATATGGTGGCCTTCATGTTCTATGTAATGCATAGACCCTTTAGCAGTTCATCAAATGCAGTTAACAATTTCTTGGGGATATCACAAGCTTCTTCTGTTCTAGGTGGTTTTCTAGCTGATGCATATCTTGGTCGATATTGGACTATAGCAATCTTCACAACTATCTATCTTGCAGGTTTGACAGGGATAACTTTATGTGCAACAATGGACATTTTGACACCGAACCAAGATCAATGTGATCAGCTATCCCTTCTTTTAGGCAATTGTGAGCCAGCAAAATCATGGCAGATGCTTTATCTCTATTCTGTTTTGTACTTAACTGGATTTGGAGCTGCCGGTATAAGGCCATGTGTGTCATCTTTTGGGGCTGATCAGTTTGACGAAACAAGCCCAGATTACAAGTCTCACTTGGATAGGTTTTTCAACTTCTTTTATCTGTCTGTCACCATTGGAGCTATTATAGCCTTCACTGCAGTAGTGTACATTCAAATGGAACATGGTTGGGGACTTGCCTTTGGGTCACTGGCCATAGCTATGGGCATATCAAACTTTGTGTTCTTTCTTGGTACACCTCTCTACCGGCATAGACTACCCGGAGGAAGCCCTCTGACACGTGTTGCGCAAGTATTGGTGGCTGCCTACAGGAAGAGGAAGGCTGCATTTTCTAGCAGTGAGTTGATAGGCTTGTATGAGGTTCCTGGAAAACAATCTGCTATAAAGGGTAGTAGAAAGATCGCTCACACCAATGATTTTAGATGTTTAGACAAAGCAGCTCTGCAGCTTAAGGAAGACGGTGTAGACCCAACTCCTTGGAGGCTTTGCACTGTGACTCAAGTAGAGGAAGTAAAGATTCTACTTAAACTTATTCCAATCCCGGCTTGCACAATAATGCTGAGTGTAGTCTTAACTGAGTATCTGACTCTCTCAGTTCAGCAAGCTTACACTCTAAATACCCATATGGGTCACCTCAAGCTTCCAGTAACATGCATGCCAGTATTTCCAGGCCTCAGCATATTTCTCATACTATCCCTCTACTACTCTACATTTGTCCCCTTGTCGCGGCACATCACAGGTCATCCTCACGGTGCTTCTCAGTTACAGAGGGTAGGCATAGGTCTGGCAGTTTCCATCTTATCCGTGGCATGGGCCGCGGTTTTTGAGAGGTACCGAAGAAACTATGCAATAAAGCATGAGTATGAGGCTAGTTTCCTAACTGCAATGCCTGATCTAAGCGCATACTGGTTGTTGATCCAGTACTGCCTCATTGGCATAGCTGAAGTGTTTTGCATAGTGGGGTTACTAGAATTCCTCTATGAGGAAGCCCCAGATGCTATGAAAAGTATAGGGTCTGCTTATGCCGCACTAGCCGGGGGTTTAGGGTGCTTTGCAGCCAGCATATTGAACAGCATCATCAAATCTGTCACAGGCAGCACAGAGAAGCCATCTTGGCTGTCACAGAATATCAATACTGGTAGATTTGATTATTTCTACTGGCTACTTACAGTTTTGAGTGTCATCAATTTCTGCATATTTCTGTATTCGGCACATAGGTACAAATACAGGGCAGAGCAGCAAATTGTAACCAAGATTAAAGAAGACCTCATCCAAGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PTR2

Protein Analysis

633

Amino Acids

70.04

Weight (kDa)

8.13

Isoelectric Point (pI)

32.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFS_1	PF07690	92 - 559	1.4e-10	Major Facilitator Superfamily
PTR2	PF00854	141 - 578	1.2e-80	POT family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004424)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13400
fragaria_vesca	FvH4_4g22520 FvH4_4g22520 FvH4_4g22520 FvH4_4g22520 FvH4_4g22520 FvH4_4g22520 FvH4_4g22520 FvH4_4g22520 FvH4_4g22520
malus_domestica	MD13G1131800.v1.1 MD16G1142100.v1.1
prunus_persica	Prupe.1G213600_v2.0.a1 Prupe.1G213600_v2.0.a1 Prupe.1G213600_v2.0.a1 Prupe.1G213600_v2.0.a1
pyrus_communis	pycom13g11480 pycom16g11260
rosa_chinensis	RchiOBHm_Chr4g0428751
rosa_laevigata	RLG00000007129
rosa_multiflora	Rmu_co8494165.1_g000001 Rmu_sc0010032.1_g000002
rosa_roxburghii	Rroxscaffold_5G00370410
rosa_rugosa	Rorug04G0228000 Rorug04G0228100 Rorug04G0228100 Rorug04G0228200 Rorug04G0228300
rosa_samantha	Rh4AG285400 Rh4BG291400 Rh4DG288500
rosa_wichuraiana	Rw4G024750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	906
Acc36I	ACCTGC	1 cut(s)	449
Acc65I	GGTACC	1 cut(s)	1449
AccB1I	GGYRCC	1 cut(s)	1449
AccI	GTMKAC	1 cut(s)	1083
AccII	CGCG	2 cut(s)	1345, 1436
AciI	CCGC	4 cut(s)	1345, 1434, 1436, 1644
AclWI	GGATC	1 cut(s)	1528
AcoI	YGGCCR	2 cut(s)	252, 810
AcsI	RAATTY	1 cut(s)	1589
AcuI	CTGAAG	2 cut(s)	48, 1581
AcvI	CACGTG	1 cut(s)	899
AfaI	GTAC	6 cut(s)	599, 767, 853, 1451, 1541, 1838
AfiI	CCNNNNNNNGG	7 cut(s)	32, 123, 216, 627, 1344, 1428, 1600
AflII	CTTAAG	1 cut(s)	1067
AflIII	ACRYGT	2 cut(s)	896, 898
AgeI	ACCGGT	2 cut(s)	166, 209
AgsI	TTSAA	3 cut(s)	709, 773, 1687
AjnI	CCWGG	2 cut(s)	982, 1285
Alw26I	GTCTC	2 cut(s)	35, 693
AlwI	GGATC	1 cut(s)	1528
AoxI	GGCC	6 cut(s)	252, 294, 629, 810, 1288, 1431
ApeKI	GCWGC	9 cut(s)	224, 617, 920, 941, 1055, 1063, 1673, 1713, 1855
ApoI	RAATTY	1 cut(s)	1589
AsiGI	ACCGGT	2 cut(s)	166, 209
Asp718I	GGTACC	1 cut(s)	1449
AspLEI	GCGC	2 cut(s)	907, 1520
AspS9I	GGNCC	1 cut(s)	1431
AsuC2I	CCSGG	3 cut(s)	879, 1160, 1654
AsuHPI	GGTGA	2 cut(s)	721, 1241
BalI	TGGCCA	1 cut(s)	812
BanI	GGYRCC	1 cut(s)	1449
BarI	GAAGNNNNNNTAC	2 cut(s)	133, 165
BbrPI	CACGTG	1 cut(s)	899
BbsI	GAAGAC	2 cut(s)	1080, 1887
BbvCI	CCTCAGC	1 cut(s)	1292
BbvI	GCAGC	9 cut(s)	211, 604, 907, 928, 1067, 1075, 1685, 1725, 1867
BccI	CCATC	2 cut(s)	1421, 1735
BcgI	CGANNNNNNTGC	2 cut(s)	172, 206
BciT130I	CCWGG	2 cut(s)	984, 1287
BclI	TGATCA	2 cut(s)	523, 655
BcnI	CCSGG	3 cut(s)	879, 1160, 1654
BcoDI	GTCTC	2 cut(s)	35, 693
BfaI	CTAG	6 cut(s)	386, 398, 951, 1488, 1586, 1649
BfmI	CTRYAG	4 cut(s)	429, 756, 925, 1061
BfrI	CTTAAG	1 cut(s)	1067
BfuAI	ACCTGC	1 cut(s)	449
BglI	GCCNNNNNGGC	2 cut(s)	1551, 1732
BmcAI	AGTACT	1 cut(s)	1541
Bme1390I	CCNGG	5 cut(s)	879, 984, 1160, 1287, 1654
BmgT120I	GGNCC	1 cut(s)	1431
BmiI	GGNNCC	3 cut(s)	160, 981, 1451
BmrFI	CCNGG	5 cut(s)	879, 984, 1160, 1287, 1654
BmsI	GCATC	4 cut(s)	394, 564, 1603, 1701
BpiI	GAAGAC	2 cut(s)	1080, 1887
BpmI	CTGGAG	1 cut(s)	30
Bpu10I	CCTNAGC	1 cut(s)	1292
BpuEI	CTTGAG	2 cut(s)	1101, 1238
BpuMI	CCSGG	3 cut(s)	879, 1160, 1654
BsaAI	YACGTR	1 cut(s)	899
BsaBI	GATNNNNATC	1 cut(s)	408
BsaJI	CCNNGG	7 cut(s)	83, 178, 1094, 1422, 1434, 1653, 1891
BsaWI	WCCGGW	3 cut(s)	152, 166, 209
Bsc4I	CCNNNNNNNGG	7 cut(s)	32, 123, 216, 627, 1344, 1428, 1600
Bse118I	RCCGGY	4 cut(s)	166, 209, 620, 864
Bse1I	ACTGG	8 cut(s)	610, 813, 1262, 1277, 1529, 1537, 1759, 1781
Bse3DI	GCAATG	1 cut(s)	1509
Bse8I	GATNNNNATC	1 cut(s)	408
BseBI	CCWGG	2 cut(s)	984, 1287
BseDI	CCNNGG	7 cut(s)	83, 178, 1094, 1422, 1434, 1653, 1891
BseGI	GGATG	2 cut(s)	1360, 1887
BseJI	GATNNNNATC	1 cut(s)	408
BseLI	CCNNNNNNNGG	7 cut(s)	32, 123, 216, 627, 1344, 1428, 1600
BseMI	GCAATG	1 cut(s)	1509
BseMII	CTCAG	6 cut(s)	294, 1169, 1185, 1224, 1306, 1391
BseNI	ACTGG	8 cut(s)	610, 813, 1262, 1277, 1529, 1537, 1759, 1781
BseXI	GCAGC	9 cut(s)	211, 604, 907, 928, 1067, 1075, 1685, 1725, 1867
Bsh1236I	CGCG	2 cut(s)	1345, 1436
BshFI	GGCC	6 cut(s)	254, 296, 631, 812, 1290, 1433
BshNI	GGYRCC	1 cut(s)	1449
BshTI	ACCGGT	2 cut(s)	166, 209
BsiSI	CCGG	8 cut(s)	153, 167, 210, 621, 865, 879, 1160, 1653
BslFI	GGGAC	3 cut(s)	7, 804, 1319
BslI	CCNNNNNNNGG	7 cut(s)	32, 123, 216, 627, 1344, 1428, 1600
BsmAI	GTCTC	2 cut(s)	35, 693
BsmFI	GGGAC	3 cut(s)	7, 804, 1319
BsnI	GGCC	6 cut(s)	254, 296, 631, 812, 1290, 1433
Bsp1407I	TGTACA	1 cut(s)	765
Bsp143I	GATC	7 cut(s)	196, 514, 523, 655, 1017, 1510, 1533
Bsp19I	CCATGG	1 cut(s)	178
BspACI	CCGC	4 cut(s)	1345, 1434, 1436, 1644
BspANI	GGCC	6 cut(s)	254, 296, 631, 812, 1290, 1433
BspCNI	CTCAG	6 cut(s)	293, 1170, 1186, 1223, 1305, 1390
BspFNI	CGCG	2 cut(s)	1345, 1436
BspLI	GGNNCC	3 cut(s)	160, 981, 1451
BspMAI	CTGCAG	2 cut(s)	760, 1065
BspMI	ACCTGC	1 cut(s)	449
BspPI	GGATC	1 cut(s)	1528
BspT107I	GGYRCC	1 cut(s)	1449
BspTI	CTTAAG	1 cut(s)	1067
BsrDI	GCAATG	1 cut(s)	1509
BsrFI	RCCGGY	4 cut(s)	166, 209, 620, 864
BsrGI	TGTACA	1 cut(s)	765
BsrI	ACTGG	8 cut(s)	610, 813, 1262, 1277, 1529, 1537, 1759, 1781
BssAI	RCCGGY	4 cut(s)	166, 209, 620, 864
BssECI	CCNNGG	7 cut(s)	83, 178, 1094, 1422, 1434, 1653, 1891
BssMI	GATC	7 cut(s)	196, 514, 523, 655, 1017, 1510, 1533
BssT1I	CCWWGG	4 cut(s)	83, 178, 1094, 1891
Bst2UI	CCWGG	2 cut(s)	984, 1287
Bst4CI	ACNGT	4 cut(s)	1079, 1111, 1370, 1789
Bst6I	CTCTTC	1 cut(s)	926
BstAFI	CTTAAG	1 cut(s)	1067
BstAUI	TGTACA	1 cut(s)	765
BstBAI	YACGTR	1 cut(s)	899
BstC8I	GCNNGC	5 cut(s)	558, 1165, 1221, 1274, 1678
BstDEI	CTNAG	7 cut(s)	280, 1178, 1194, 1210, 1292, 1377, 1514
BstDSI	CCRYGG	3 cut(s)	178, 1422, 1434
BstEII	GGTNACC	1 cut(s)	1247
BstENI	CCTNNNNNAGG	2 cut(s)	30, 1598
BstF5I	GGATG	2 cut(s)	1360, 1887
BstFNI	CGCG	2 cut(s)	1345, 1436
BstHHI	GCGC	2 cut(s)	907, 1520
BstKTI	GATC	7 cut(s)	199, 517, 526, 658, 1020, 1513, 1536
BstMAI	GTCTC	2 cut(s)	35, 693
BstMBI	GATC	7 cut(s)	196, 514, 523, 655, 1017, 1510, 1533
BstMWI	GCNNNNNNNGC	8 cut(s)	746, 755, 1433, 1551, 1613, 1643, 1649, 1732
BstNI	CCWGG	2 cut(s)	984, 1287
BstNSI	RCATGY	2 cut(s)	1272, 1276
BstPI	GGTNACC	1 cut(s)	1247
BstSCI	CCNGG	5 cut(s)	877, 982, 1158, 1285, 1652
BstSFI	CTRYAG	4 cut(s)	429, 756, 925, 1061
BstUI	CGCG	2 cut(s)	1345, 1436
BstV1I	GCAGC	9 cut(s)	211, 604, 907, 928, 1067, 1075, 1685, 1725, 1867
BstV2I	GAAGAC	2 cut(s)	1080, 1887
BstXI	CCANNNNNNTGG	2 cut(s)	820, 1095
BsuRI	GGCC	6 cut(s)	254, 296, 631, 812, 1290, 1433
BtgI	CCRYGG	3 cut(s)	178, 1422, 1434
BtsCI	GGATG	2 cut(s)	1360, 1887
BtsI	GCAGTG	2 cut(s)	753, 961
BtsIMutI	CAGTG	5 cut(s)	288, 753, 806, 961, 1107
BveI	ACCTGC	1 cut(s)	449
Cac8I	GCNNGC	5 cut(s)	558, 1165, 1221, 1274, 1678
CfoI	GCGC	2 cut(s)	907, 1520
Cfr10I	RCCGGY	4 cut(s)	166, 209, 620, 864
Cfr13I	GGNCC	1 cut(s)	1431
Cfr42I	CCGCGG	1 cut(s)	1437
Csp6I	GTAC	6 cut(s)	598, 766, 852, 1450, 1540, 1837
CspAI	ACCGGT	2 cut(s)	166, 209
CspCI	CAANNNNNGTGG	2 cut(s)	193, 228
CviAII	CATG	9 cut(s)	179, 301, 567, 633, 782, 1269, 1273, 1428, 1475
CviQI	GTAC	6 cut(s)	598, 766, 852, 1450, 1540, 1837
DdeI	CTNAG	7 cut(s)	280, 1178, 1194, 1210, 1292, 1377, 1514
DpnI	GATC	7 cut(s)	198, 516, 525, 657, 1019, 1512, 1535
DpnII	GATC	7 cut(s)	196, 514, 523, 655, 1017, 1510, 1533
EaeI	YGGCCR	2 cut(s)	252, 810
Eam1104I	CTCTTC	1 cut(s)	926
EarI	CTCTTC	1 cut(s)	926
Eco130I	CCWWGG	4 cut(s)	83, 178, 1094, 1891
Eco147I	AGGCCT	1 cut(s)	1290
Eco32I	GATATC	1 cut(s)	366
Eco57I	CTGAAG	2 cut(s)	48, 1581
Eco72I	CACGTG	1 cut(s)	899
Eco91I	GGTNACC	1 cut(s)	1247
EcoNI	CCTNNNNNAGG	2 cut(s)	30, 1598
EcoO65I	GGTNACC	1 cut(s)	1247
EcoRI	GAATTC	1 cut(s)	1589
EcoRII	CCWGG	2 cut(s)	982, 1285
EcoRV	GATATC	1 cut(s)	366
EcoT14I	CCWWGG	4 cut(s)	83, 178, 1094, 1891
EcoT22I	ATGCAT	3 cut(s)	318, 409, 1274
ErhI	CCWWGG	4 cut(s)	83, 178, 1094, 1891
FaeI	CATG	9 cut(s)	182, 304, 570, 636, 785, 1272, 1276, 1431, 1478
FalI	AAGNNNNNCTT	2 cut(s)	677, 709
FaqI	GGGAC	3 cut(s)	7, 804, 1319
FatI	CATG	9 cut(s)	178, 300, 566, 632, 781, 1268, 1272, 1427, 1474
FauNDI	CATATG	1 cut(s)	1242
FbaI	TGATCA	2 cut(s)	523, 655
FblI	GTMKAC	1 cut(s)	1083
FokI	GGATG	2 cut(s)	1347, 1874
FspBI	CTAG	6 cut(s)	386, 398, 951, 1488, 1586, 1649
FspI	TGCGCA	1 cut(s)	906
GlaI	GCGC	2 cut(s)	906, 1519
GsuI	CTGGAG	1 cut(s)	30
HaeIII	GGCC	6 cut(s)	254, 296, 631, 812, 1290, 1433
HapII	CCGG	8 cut(s)	153, 167, 210, 621, 865, 879, 1160, 1653
HhaI	GCGC	2 cut(s)	907, 1520
Hin1II	CATG	9 cut(s)	182, 304, 570, 636, 785, 1272, 1276, 1431, 1478
Hin6I	GCGC	2 cut(s)	905, 1518
HinP1I	GCGC	2 cut(s)	905, 1518
HincII	GTYRAC	1 cut(s)	349
HindII	GTYRAC	1 cut(s)	349
HindIII	AAGCTT	4 cut(s)	92, 372, 1221, 1256
HinfI	GANTC	6 cut(s)	43, 113, 276, 1114, 1135, 1204
HpaI	GTTAAC	1 cut(s)	349
HpaII	CCGG	8 cut(s)	153, 167, 210, 621, 865, 879, 1160, 1653
HphI	GGTGA	2 cut(s)	721, 1241
Hpy166II	GTNNAC	4 cut(s)	349, 766, 854, 1084
Hpy188I	TCNGA	4 cut(s)	73, 118, 894, 1203
Hpy8I	GTNNAC	4 cut(s)	349, 766, 854, 1084
HpyAV	CCTTC	7 cut(s)	23, 135, 238, 307, 545, 760, 931
HpyCH4III	ACNGT	4 cut(s)	1079, 1111, 1370, 1789
HpyCH4IV	ACGT	1 cut(s)	898
HpyF10VI	GCNNNNNNNGC	8 cut(s)	746, 755, 1433, 1551, 1613, 1643, 1649, 1732
HpyF3I	CTNAG	7 cut(s)	280, 1178, 1194, 1210, 1292, 1377, 1514
HpySE526I	ACGT	1 cut(s)	898
Hsp92II	CATG	9 cut(s)	182, 304, 570, 636, 785, 1272, 1276, 1431, 1478
HspAI	GCGC	2 cut(s)	905, 1518
KpnI	GGTACC	1 cut(s)	1453
Ksp22I	TGATCA	2 cut(s)	523, 655
KspAI	GTTAAC	1 cut(s)	349
KspI	CCGCGG	1 cut(s)	1437
Kzo9I	GATC	7 cut(s)	196, 514, 523, 655, 1017, 1510, 1533
LmnI	GCTCC	2 cut(s)	614, 737
Lsp1109I	GCAGC	9 cut(s)	211, 604, 907, 928, 1067, 1075, 1685, 1725, 1867
LweI	GCATC	4 cut(s)	394, 564, 1603, 1701
MaeI	CTAG	6 cut(s)	386, 398, 951, 1488, 1586, 1649
MaeII	ACGT	1 cut(s)	898
MalI	GATC	7 cut(s)	198, 516, 525, 657, 1019, 1512, 1535
MboI	GATC	7 cut(s)	196, 514, 523, 655, 1017, 1510, 1533
MboII	GAAGA	8 cut(s)	100, 223, 369, 430, 943, 1085, 1467, 1892
MfeI	CAATTG	1 cut(s)	547
MlsI	TGGCCA	1 cut(s)	812
MluCI	AATT	6 cut(s)	52, 352, 547, 1589, 1804, 1860
MluNI	TGGCCA	1 cut(s)	812
MlyI	GAGTC	4 cut(s)	37, 270, 1108, 1198
Mox20I	TGGCCA	1 cut(s)	812
Mph1103I	ATGCAT	3 cut(s)	318, 409, 1274
MscI	TGGCCA	1 cut(s)	812
MseI	TTAA	7 cut(s)	171, 348, 602, 1068, 1143, 1190, 1875
MslI	CAYNNNNRTG	5 cut(s)	773, 818, 1029, 1172, 1479
Msp20I	TGGCCA	1 cut(s)	812
MspA1I	CMGCKG	1 cut(s)	1436
MspCI	CTTAAG	1 cut(s)	1067
MspI	CCGG	8 cut(s)	153, 167, 210, 621, 865, 879, 1160, 1653
MspR9I	CCNGG	5 cut(s)	879, 984, 1160, 1287, 1654
MunI	CAATTG	1 cut(s)	547
MvaI	CCWGG	2 cut(s)	984, 1287
MvnI	CGCG	2 cut(s)	1345, 1436
MwoI	GCNNNNNNNGC	8 cut(s)	746, 755, 1433, 1551, 1613, 1643, 1649, 1732
NciI	CCSGG	3 cut(s)	879, 1160, 1654
NcoI	CCATGG	1 cut(s)	178
NdeI	CATATG	1 cut(s)	1242
NdeII	GATC	7 cut(s)	196, 514, 523, 655, 1017, 1510, 1533
NlaIII	CATG	9 cut(s)	182, 304, 570, 636, 785, 1272, 1276, 1431, 1478
NlaIV	GGNNCC	3 cut(s)	160, 981, 1451
NmeAIII	GCCGAG	1 cut(s)	280
NmuCI	GTSAC	6 cut(s)	727, 804, 1111, 1247, 1705, 1737
NsbI	TGCGCA	1 cut(s)	906
NsiI	ATGCAT	3 cut(s)	318, 409, 1274
NspI	RCATGY	2 cut(s)	1272, 1276
PaeI	GCATGC	1 cut(s)	1276
PceI	AGGCCT	1 cut(s)	1290
PfeI	GAWTC	2 cut(s)	113, 1135
PfoI	TCCNGGA	1 cut(s)	982
PinAI	ACCGGT	2 cut(s)	166, 209
PleI	GAGTC	4 cut(s)	37, 270, 1108, 1198
PmaCI	CACGTG	1 cut(s)	899
PmlI	CACGTG	1 cut(s)	899
PpsI	GAGTC	4 cut(s)	37, 270, 1108, 1198
Ppu21I	YACGTR	1 cut(s)	899
Psp6I	CCWGG	2 cut(s)	982, 1285
PspCI	CACGTG	1 cut(s)	899
PspEI	GGTNACC	1 cut(s)	1247
PspGI	CCWGG	2 cut(s)	982, 1285
PspN4I	GGNNCC	3 cut(s)	160, 981, 1451
PspPI	GGNCC	1 cut(s)	1431
PstI	CTGCAG	2 cut(s)	760, 1065
RsaI	GTAC	6 cut(s)	599, 767, 853, 1451, 1541, 1838
RsaNI	GTAC	6 cut(s)	598, 766, 852, 1450, 1540, 1837
RseI	CAYNNNNRTG	5 cut(s)	773, 818, 1029, 1172, 1479
SacII	CCGCGG	1 cut(s)	1437
SaqAI	TTAA	7 cut(s)	171, 348, 602, 1068, 1143, 1190, 1875
Sau3AI	GATC	7 cut(s)	196, 514, 523, 655, 1017, 1510, 1533
Sau96I	GGNCC	1 cut(s)	1431
ScaI	AGTACT	1 cut(s)	1541
SchI	GAGTC	4 cut(s)	37, 270, 1108, 1198
ScrFI	CCNGG	5 cut(s)	879, 984, 1160, 1287, 1654
SfaNI	GCATC	4 cut(s)	394, 564, 1603, 1701
SfcI	CTRYAG	4 cut(s)	429, 756, 925, 1061
Sfr303I	CCGCGG	1 cut(s)	1437
SgrBI	CCGCGG	1 cut(s)	1437
SmiMI	CAYNNNNRTG	5 cut(s)	773, 818, 1029, 1172, 1479
SmlI	CTYRAG	3 cut(s)	1067, 1116, 1253
SmoI	CTYRAG	3 cut(s)	1067, 1116, 1253
SphI	GCATGC	1 cut(s)	1276
Sse9I	AATT	6 cut(s)	52, 352, 547, 1589, 1804, 1860
SseBI	AGGCCT	1 cut(s)	1290
SsiI	CCGC	4 cut(s)	1345, 1434, 1436, 1644
SspI	AATATT	1 cut(s)	68
SspMI	CTAG	6 cut(s)	386, 398, 951, 1488, 1586, 1649
StuI	AGGCCT	1 cut(s)	1290
StyD4I	CCNGG	5 cut(s)	877, 982, 1158, 1285, 1652
StyI	CCWWGG	4 cut(s)	83, 178, 1094, 1891
TaaI	ACNGT	4 cut(s)	1079, 1111, 1370, 1789
TaiI	ACGT	1 cut(s)	901
TaqI	TCGA	2 cut(s)	111, 418
TasI	AATT	6 cut(s)	52, 352, 547, 1589, 1804, 1860
TatI	WGTACW	3 cut(s)	597, 765, 1539
TauI	GCSGC	3 cut(s)	1348, 1436, 1646
TfiI	GAWTC	2 cut(s)	113, 1135
Tru1I	TTAA	7 cut(s)	171, 348, 602, 1068, 1143, 1190, 1875
Tru9I	TTAA	7 cut(s)	171, 348, 602, 1068, 1143, 1190, 1875
TscAI	CASTG	5 cut(s)	288, 760, 813, 961, 1114
TseFI	GTSAC	6 cut(s)	727, 804, 1111, 1247, 1705, 1737
TseI	GCWGC	9 cut(s)	224, 617, 920, 941, 1055, 1063, 1673, 1713, 1855
Tsp45I	GTSAC	6 cut(s)	727, 804, 1111, 1247, 1705, 1737
TspDTI	ATGAA	6 cut(s)	97, 223, 261, 289, 324, 1634
TspGWI	ACGGA	1 cut(s)	1411
TspRI	CASTG	5 cut(s)	288, 760, 813, 961, 1114
Vha464I	CTTAAG	1 cut(s)	1067
XagI	CCTNNNNNAGG	2 cut(s)	30, 1598
XapI	RAATTY	1 cut(s)	1589
XceI	RCATGY	2 cut(s)	1272, 1276
XcmI	CCANNNNNNNNNTGG	2 cut(s)	564, 1420
XmiI	GTMKAC	1 cut(s)	1083
XspI	CTAG	6 cut(s)	386, 398, 951, 1488, 1586, 1649
ZrmI	AGTACT	1 cut(s)	1541
Zsp2I	ATGCAT	3 cut(s)	318, 409, 1274

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.