Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004424

Physical Location & Seq

Genomic Coordinates Forward (+)

10938991 .. 10941873

Gene Structure 2883 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1142100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1875 bp

ATGACACCAGCTAGTTTAGATGGAAATCCAGACTCAAACCAGAGAAAGAAGCTTGGACTGTACTTCATTGAATCCGATGACAGGCGGATGGCTTTCGGACGCGGTTATACTGGAGGAACCACACCAGTTGACATCCATGGAAAACCTATTGCTGATCTTTCGAAAACCGGCGGTTGGTTTGCTGCCTTCTTCATATTTGGAAATGAAATGGCTGAGAGAATGGCGTATTTCGGTCTCTCGGTTAACATGGTGGCCTTCATGTTTTATGTTATGCATAGACCCTTCAGCAGTTCATCAAATGCAGTTAACAATTTTCTTGGGATTTCACAAGCTTCATCTGTCCTCGGCGGTTTTCTAGCTGATGCATATCTTGGTCGATATTGGACAATAGCAATCTTTACAACTATCTATCTTGTGGGTTTGACAGGAATAACCTTATGTGCAACGATGCACAATTTCGTGCCAAACCAAGATCAATGCAGCGAGCTAGCCCTCCTTTTGGGCAATTGTGAGCCTGCAAAACCGTGGCAGATGCTTTACCTCTACACGGTTCTATACATAACTGGATTCGGAGCTGCTGGCATAAGGCCGTGCGTTTCCTCTTTCGGGGCAGACCAGTTCGACGAAAGAAGTAGAGATTACAAGTCTCACCTGGATAGGTTTTTCAACTTCTTTTATCTCTCTGTCACCATTGGGGCAATTATCGCCTTCACTGCAGTAGTCTATGTCCAAATGAAACTTGGTTGGGGATTCGCCTTTGGGTCACTGGCATTGGCAATGGGCATATCAAACGTGGTCTTCTTTCTAGGCACCCCTCTATACCGGCATAGACTGCCAGGAGGCAGCCCTTTAACGCGCGTTGCTCAAGTTTTGGTTGCTGCCTACAAGAAGAGGAACGCAGAATTTTCCAGCAGTGAGTTGATAGGCTTGTATGAGGTTCCTGGAAAACAATCTGCCATAAAGGGTAGTGGAAAGATAGCTCACACCAACGATTTTAGATGTTTGGACAAAGCAGCTCTGCAATTGAAGGAAGATGGCGTAGACCCGAGTCCCTGGAGGCTATGCACTGTGACACAAGTAGAGGAAGTGAAGATCTTGTTGAAACTTATTCCGATCCCGGCATGCACAATAATTTTGAGTGTAGTTTTGACAGAATATCTCACCCTCTCAGTGCAGCAGGCATACACTTTAAACACCCATATAGGCAAACTGAAACTCCCTGTAACATGCATGCCAGTGTTTCCTGGCCTCAGTATCTTTCTCATACTGTCCCTCTACTACTCTACATTTGTCCCACTGTCCAGGCGCATCACCGGCCATCCTCACGGTGCTTCGCAGCTTCAGAGGGTGGGGATAGGTCTAGCAGTTTCAATCCTGTCTGTGGCGTGGGCTGCGATATTCGAAATGTACCGGAGAAATTATGCTATAAAGCACGGATACGAAAGTAACTTCTTAACTGCAATGCCAAATCTAAGTGCATTCTGGTTGCTTATCCAGTACTGCCTTATCGGCATAGCTGAGGTGTTTTGCATAGTGGGATTACTTGAGTTCCTGTATGAGGAAGCCCCAGATGCAATGAAGAGTATCGGATCTTCTTACGCTGCTCTTGCCGGCGGTTTAGGTTGCTTTGCAGCCAGTATATTGAACAGCATCATCAAATCTGTCACCGGAAGCTCAGAGAAGCCGTCTTGGCTGTCCCAGAATATCAACACAGGAAGGTTTGATTATTTCTACTGGCTGCTGACAGTTTTGAGTCTCATCAATTTCGGCGTTTTTCTGTATTGCGCGCATCGCTACAAGTACAGGACGGAGCAAGGGGTTAAGACGGAGAAGCAGGCTCTAACCAACGTAAAAGAACAACCCCTCAGTGGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PTR2

Protein Analysis

625

Amino Acids

68.93

Weight (kDa)

8.87

Isoelectric Point (pI)

34.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFS_1	PF07690	78 - 545	1.9e-10	Major Facilitator Superfamily
PTR2	PF00854	127 - 564	5e-79	POT family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004424)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13400
fragaria_vesca	FvH4_4g22520 FvH4_4g22520 FvH4_4g22520 FvH4_4g22520 FvH4_4g22520 FvH4_4g22520 FvH4_4g22520 FvH4_4g22520 FvH4_4g22520
malus_domestica	MD13G1131800.v1.1 MD16G1142100.v1.1
prunus_persica	Prupe.1G213600_v2.0.a1 Prupe.1G213600_v2.0.a1 Prupe.1G213600_v2.0.a1 Prupe.1G213600_v2.0.a1
pyrus_communis	pycom13g11480 pycom16g11260
rosa_chinensis	RchiOBHm_Chr4g0428751
rosa_laevigata	RLG00000007129
rosa_multiflora	Rmu_co8494165.1_g000001 Rmu_sc0010032.1_g000002
rosa_roxburghii	Rroxscaffold_5G00370410
rosa_rugosa	Rorug04G0228000 Rorug04G0228100 Rorug04G0228100 Rorug04G0228200 Rorug04G0228300
rosa_samantha	Rh4AG285400 Rh4BG291400 Rh4DG288500
rosa_wichuraiana	Rw4G024750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	809
AccI	GTMKAC	1 cut(s)	1041
AccII	CGCG	4 cut(s)	102, 856, 858, 1787
AciI	CCGC	5 cut(s)	85, 102, 171, 348, 1614
AclWI	GGATC	2 cut(s)	1108, 1597
AcoI	YGGCCR	1 cut(s)	1315
AcsI	RAATTY	1 cut(s)	902
AcuI	CTGAAG	2 cut(s)	268, 1325
AfaI	GTAC	4 cut(s)	62, 1409, 1499, 1802
AfiI	CCNNNNNNNGG	8 cut(s)	81, 174, 499, 547, 606, 1381, 1558, 1868
AgsI	TTSAA	6 cut(s)	71, 667, 1027, 1102, 1371, 1645
AjnI	CCWGG	6 cut(s)	651, 835, 940, 1052, 1243, 1301
Alw26I	GTCTC	3 cut(s)	239, 651, 1760
AlwI	GGATC	2 cut(s)	1108, 1597
Ama87I	CYCGRG	1 cut(s)	1045
AoxI	GGCC	4 cut(s)	252, 587, 1246, 1315
ApoI	RAATTY	1 cut(s)	902
AspLEI	GCGC	4 cut(s)	858, 1308, 1787, 1789
AsuC2I	CCSGG	1 cut(s)	1118
AsuHPI	GGTGA	5 cut(s)	641, 679, 1153, 1303, 1657
AsuII	TTCGAA	2 cut(s)	161, 1401
AsuNHI	GCTAGC	1 cut(s)	487
AvaI	CYCGRG	1 cut(s)	1045
BaeI	ACNNNNGTAYC	2 cut(s)	1429, 1462
BanI	GGYRCC	1 cut(s)	809
BarI	GAAGNNNNNNTAC	2 cut(s)	1576, 1608
BbsI	GAAGAC	1 cut(s)	790
BbvCI	CCTCAGC	1 cut(s)	1518
BccI	CCATC	4 cut(s)	14, 82, 1028, 1326
BceAI	ACGGC	2 cut(s)	574, 1669
BciT130I	CCWGG	6 cut(s)	653, 837, 942, 1054, 1245, 1303
BciVI	GTATCC	1 cut(s)	1430
BcnI	CCSGG	1 cut(s)	1118
BcoDI	GTCTC	3 cut(s)	239, 651, 1760
BfaI	CTAG	5 cut(s)	12, 356, 488, 806, 1361
BfmI	CTRYAG	1 cut(s)	714
BfuI	GTATCC	1 cut(s)	1430
BglI	GCCNNNNNGGC	2 cut(s)	1509, 1690
BglII	AGATCT	1 cut(s)	1092
BmcAI	AGTACT	1 cut(s)	1499
Bme1390I	CCNGG	7 cut(s)	653, 837, 942, 1054, 1118, 1245, 1303
BmeT110I	CYCGRG	1 cut(s)	1045
BmiI	GGNNCC	3 cut(s)	118, 811, 939
BmrFI	CCNGG	7 cut(s)	653, 837, 942, 1054, 1118, 1245, 1303
BmsI	GCATC	7 cut(s)	352, 438, 522, 1317, 1561, 1659, 1798
BmtI	GCTAGC	1 cut(s)	491
BoxI	GACNNNNGTC	1 cut(s)	1047
BpiI	GAAGAC	1 cut(s)	790
BpmI	CTGGAG	2 cut(s)	132, 1075
Bpu10I	CCTNAGC	1 cut(s)	1518
Bpu14I	TTCGAA	2 cut(s)	161, 1401
BpuEI	CTTGAG	2 cut(s)	849, 1565
BpuMI	CCSGG	1 cut(s)	1118
BsaBI	GATNNNNATC	2 cut(s)	24, 366
BsaI	GGTCTC	1 cut(s)	239
BsaJI	CCNNGG	4 cut(s)	136, 343, 524, 1052
BsaWI	WCCGGW	2 cut(s)	1410, 1667
BsaXI	ACNNNNNCTCC	2 cut(s)	1200, 1230
Bsc4I	CCNNNNNNNGG	8 cut(s)	81, 174, 499, 547, 606, 1381, 1558, 1868
Bse118I	RCCGGY	4 cut(s)	167, 822, 1313, 1610
Bse1I	ACTGG	9 cut(s)	115, 125, 568, 616, 771, 1235, 1495, 1635, 1739
Bse3DI	GCAATG	3 cut(s)	783, 1467, 1581
Bse8I	GATNNNNATC	2 cut(s)	24, 366
BseBI	CCWGG	6 cut(s)	653, 837, 942, 1054, 1245, 1303
BseDI	CCNNGG	4 cut(s)	136, 343, 524, 1052
BseGI	GGATG	3 cut(s)	93, 132, 1318
BseJI	GATNNNNATC	2 cut(s)	24, 366
BseLI	CCNNNNNNNGG	8 cut(s)	81, 174, 499, 547, 606, 1381, 1558, 1868
BseMI	GCAATG	3 cut(s)	783, 1467, 1581
BseMII	CTCAG	5 cut(s)	204, 1182, 1264, 1509, 1689
BseNI	ACTGG	9 cut(s)	115, 125, 568, 616, 771, 1235, 1495, 1635, 1739
BsePI	GCGCGC	1 cut(s)	1785
BsgI	GTGCAG	1 cut(s)	1193
Bsh1236I	CGCG	4 cut(s)	102, 856, 858, 1787
BshFI	GGCC	4 cut(s)	254, 589, 1248, 1317
BshNI	GGYRCC	1 cut(s)	809
BsiHKCI	CYCGRG	1 cut(s)	1045
BsiSI	CCGG	7 cut(s)	168, 823, 1118, 1314, 1411, 1611, 1668
BslFI	GGGAC	4 cut(s)	1035, 1255, 1277, 1681
BslI	CCNNNNNNNGG	8 cut(s)	81, 174, 499, 547, 606, 1381, 1558, 1868
BsmAI	GTCTC	3 cut(s)	239, 651, 1760
BsmFI	GGGAC	4 cut(s)	1035, 1255, 1277, 1681
BsmI	GAATGC	1 cut(s)	1478
BsnI	GGCC	4 cut(s)	254, 589, 1248, 1317
Bso31I	GGTCTC	1 cut(s)	239
BsoBI	CYCGRG	1 cut(s)	1045
Bsp119I	TTCGAA	2 cut(s)	161, 1401
Bsp143I	GATC	5 cut(s)	154, 472, 1092, 1113, 1589
Bsp19I	CCATGG	1 cut(s)	136
BspACI	CCGC	5 cut(s)	85, 102, 171, 348, 1614
BspANI	GGCC	4 cut(s)	254, 589, 1248, 1317
BspCNI	CTCAG	5 cut(s)	205, 1181, 1263, 1510, 1688
BspFNI	CGCG	4 cut(s)	102, 856, 858, 1787
BspLI	GGNNCC	3 cut(s)	118, 811, 939
BspMAI	CTGCAG	1 cut(s)	718
BspOI	GCTAGC	1 cut(s)	491
BspPI	GGATC	2 cut(s)	1108, 1597
BspT104I	TTCGAA	2 cut(s)	161, 1401
BspT107I	GGYRCC	1 cut(s)	809
BspTNI	GGTCTC	1 cut(s)	239
BsrDI	GCAATG	3 cut(s)	783, 1467, 1581
BsrFI	RCCGGY	4 cut(s)	167, 822, 1313, 1610
BsrI	ACTGG	9 cut(s)	115, 125, 568, 616, 771, 1235, 1495, 1635, 1739
BssAI	RCCGGY	4 cut(s)	167, 822, 1313, 1610
BssECI	CCNNGG	4 cut(s)	136, 343, 524, 1052
BssHII	GCGCGC	1 cut(s)	1785
BssMI	GATC	5 cut(s)	154, 472, 1092, 1113, 1589
BssT1I	CCWWGG	1 cut(s)	136
Bst2UI	CCWGG	6 cut(s)	653, 837, 942, 1054, 1245, 1303
Bst4CI	ACNGT	8 cut(s)	60, 525, 550, 1069, 1269, 1299, 1328, 1747
Bst6I	CTCTTC	2 cut(s)	884, 1574
BstAPI	GCANNNNNTGC	1 cut(s)	832
BstBI	TTCGAA	2 cut(s)	161, 1401
BstDEI	CTNAG	7 cut(s)	213, 1168, 1250, 1472, 1518, 1675, 1865
BstDSI	CCRYGG	2 cut(s)	136, 524
BstENI	CCTNNNNNAGG	1 cut(s)	1556
BstF5I	GGATG	3 cut(s)	93, 132, 1318
BstFNI	CGCG	4 cut(s)	102, 856, 858, 1787
BstHHI	GCGC	4 cut(s)	858, 1308, 1787, 1789
BstKTI	GATC	5 cut(s)	157, 475, 1095, 1116, 1592
BstMAI	GTCTC	3 cut(s)	239, 651, 1760
BstMBI	GATC	5 cut(s)	154, 472, 1092, 1113, 1589
BstNI	CCWGG	6 cut(s)	653, 837, 942, 1054, 1245, 1303
BstNSI	RCATGY	3 cut(s)	1125, 1230, 1234
BstPAI	GACNNNNGTC	1 cut(s)	1047
BstSCI	CCNGG	7 cut(s)	651, 835, 940, 1052, 1116, 1243, 1301
BstSFI	CTRYAG	1 cut(s)	714
BstUI	CGCG	4 cut(s)	102, 856, 858, 1787
BstV2I	GAAGAC	1 cut(s)	790
BstX2I	RGATCY	2 cut(s)	1092, 1589
BstYI	RGATCY	2 cut(s)	1092, 1589
BsuI	GTATCC	1 cut(s)	1430
BsuRI	GGCC	4 cut(s)	254, 589, 1248, 1317
BtgI	CCRYGG	2 cut(s)	136, 524
BtgZI	GCGATG	1 cut(s)	1775
BtsCI	GGATG	3 cut(s)	93, 132, 1318
BtsI	GCAGTG	2 cut(s)	711, 919
BtsIMutI	CAGTG	8 cut(s)	711, 764, 919, 1065, 1176, 1242, 1295, 1873
CfoI	GCGC	4 cut(s)	858, 1308, 1787, 1789
Cfr10I	RCCGGY	4 cut(s)	167, 822, 1313, 1610
CseI	GACGC	1 cut(s)	108
Csp6I	GTAC	4 cut(s)	61, 1408, 1498, 1801
CspCI	CAANNNNNGTGG	2 cut(s)	109, 144
CviAII	CATG	6 cut(s)	137, 247, 259, 1122, 1227, 1231
CviQI	GTAC	4 cut(s)	61, 1408, 1498, 1801
DdeI	CTNAG	7 cut(s)	213, 1168, 1250, 1472, 1518, 1675, 1865
DpnI	GATC	5 cut(s)	156, 474, 1094, 1115, 1591
DpnII	GATC	5 cut(s)	154, 472, 1092, 1113, 1589
DraI	TTTAAA	1 cut(s)	1191
EaeI	YGGCCR	1 cut(s)	1315
Eam1104I	CTCTTC	2 cut(s)	884, 1574
EarI	CTCTTC	2 cut(s)	884, 1574
EciI	GGCGGA	1 cut(s)	100
Eco130I	CCWWGG	1 cut(s)	136
Eco31I	GGTCTC	1 cut(s)	239
Eco57I	CTGAAG	2 cut(s)	268, 1325
Eco88I	CYCGRG	1 cut(s)	1045
EcoNI	CCTNNNNNAGG	1 cut(s)	1556
EcoRII	CCWGG	6 cut(s)	651, 835, 940, 1052, 1243, 1301
EcoT14I	CCWWGG	1 cut(s)	136
EcoT22I	ATGCAT	3 cut(s)	276, 367, 1232
ErhI	CCWWGG	1 cut(s)	136
FaeI	CATG	6 cut(s)	140, 250, 262, 1125, 1230, 1234
FaqI	GGGAC	4 cut(s)	1035, 1255, 1277, 1681
FatI	CATG	6 cut(s)	136, 246, 258, 1121, 1226, 1230
FblI	GTMKAC	1 cut(s)	1041
FokI	GGATG	3 cut(s)	100, 119, 1305
FspBI	CTAG	5 cut(s)	12, 356, 488, 806, 1361
GlaI	GCGC	4 cut(s)	857, 1307, 1786, 1788
GsuI	CTGGAG	2 cut(s)	132, 1075
HaeIII	GGCC	4 cut(s)	254, 589, 1248, 1317
HapII	CCGG	7 cut(s)	168, 823, 1118, 1314, 1411, 1611, 1668
HgaI	GACGC	1 cut(s)	108
HhaI	GCGC	4 cut(s)	858, 1308, 1787, 1789
Hin1II	CATG	6 cut(s)	140, 250, 262, 1125, 1230, 1234
Hin6I	GCGC	4 cut(s)	856, 1306, 1785, 1787
HinP1I	GCGC	4 cut(s)	856, 1306, 1785, 1787
HincII	GTYRAC	3 cut(s)	130, 244, 307
HindII	GTYRAC	3 cut(s)	130, 244, 307
HindIII	AAGCTT	2 cut(s)	50, 330
HinfI	GANTC	6 cut(s)	32, 71, 567, 750, 1048, 1753
HpaI	GTTAAC	2 cut(s)	244, 307
HpaII	CCGG	7 cut(s)	168, 823, 1118, 1314, 1411, 1611, 1668
HphI	GGTGA	5 cut(s)	641, 679, 1153, 1303, 1657
Hpy166II	GTNNAC	4 cut(s)	130, 244, 307, 1042
Hpy188I	TCNGA	7 cut(s)	76, 98, 572, 1113, 1344, 1589, 1678
Hpy188III	TCNNGA	1 cut(s)	29
Hpy8I	GTNNAC	4 cut(s)	130, 244, 307, 1042
Hpy99I	CGWCG	1 cut(s)	626
HpyAV	CCTTC	6 cut(s)	196, 265, 292, 718, 1021, 1710
HpyCH4III	ACNGT	8 cut(s)	60, 525, 550, 1069, 1269, 1299, 1328, 1747
HpyCH4IV	ACGT	2 cut(s)	792, 1848
HpyF3I	CTNAG	7 cut(s)	213, 1168, 1250, 1472, 1518, 1675, 1865
HpySE526I	ACGT	2 cut(s)	792, 1848
Hsp92II	CATG	6 cut(s)	140, 250, 262, 1125, 1230, 1234
HspAI	GCGC	4 cut(s)	856, 1306, 1785, 1787
KroI	GCCGGC	1 cut(s)	1610
KroNI	GCCGGC	1 cut(s)	1612
KspAI	GTTAAC	2 cut(s)	244, 307
Kzo9I	GATC	5 cut(s)	154, 472, 1092, 1113, 1589
LmnI	GCTCC	2 cut(s)	572, 1810
LweI	GCATC	7 cut(s)	352, 438, 522, 1317, 1561, 1659, 1798
MaeI	CTAG	5 cut(s)	12, 356, 488, 806, 1361
MaeII	ACGT	2 cut(s)	792, 1848
MaeIII	GTNAC	6 cut(s)	685, 762, 1069, 1222, 1445, 1663
MalI	GATC	5 cut(s)	156, 474, 1094, 1115, 1591
MboI	GATC	5 cut(s)	154, 472, 1092, 1113, 1589
MboII	GAAGA	7 cut(s)	181, 790, 901, 1043, 1102, 1584, 1591
MfeI	CAATTG	2 cut(s)	505, 1022
MflI	RGATCY	2 cut(s)	1092, 1589
MluCI	AATT	9 cut(s)	310, 454, 505, 699, 902, 1022, 1131, 1417, 1762
MlyI	GAGTC	3 cut(s)	26, 1057, 1762
Mph1103I	ATGCAT	3 cut(s)	276, 367, 1232
MroNI	GCCGGC	1 cut(s)	1610
MseI	TTAA	6 cut(s)	243, 306, 851, 1190, 1454, 1821
MslI	CAYNNNNRTG	1 cut(s)	1235
MspI	CCGG	7 cut(s)	168, 823, 1118, 1314, 1411, 1611, 1668
MspR9I	CCNGG	7 cut(s)	653, 837, 942, 1054, 1118, 1245, 1303
MunI	CAATTG	2 cut(s)	505, 1022
Mva1269I	GAATGC	1 cut(s)	1478
MvaI	CCWGG	6 cut(s)	653, 837, 942, 1054, 1245, 1303
MvnI	CGCG	4 cut(s)	102, 856, 858, 1787
NaeI	GCCGGC	1 cut(s)	1612
NciI	CCSGG	1 cut(s)	1118
NcoI	CCATGG	1 cut(s)	136
NdeII	GATC	5 cut(s)	154, 472, 1092, 1113, 1589
NgoMIV	GCCGGC	1 cut(s)	1610
NheI	GCTAGC	1 cut(s)	487
NlaIII	CATG	6 cut(s)	140, 250, 262, 1125, 1230, 1234
NlaIV	GGNNCC	3 cut(s)	118, 811, 939
NmeAIII	GCCGAG	1 cut(s)	324
NmuCI	GTSAC	4 cut(s)	685, 762, 1069, 1663
NsiI	ATGCAT	3 cut(s)	276, 367, 1232
NspI	RCATGY	3 cut(s)	1125, 1230, 1234
NspV	TTCGAA	2 cut(s)	161, 1401
PaeI	GCATGC	2 cut(s)	1125, 1234
PauI	GCGCGC	1 cut(s)	1785
PctI	GAATGC	1 cut(s)	1478
PdiI	GCCGGC	1 cut(s)	1612
PfeI	GAWTC	3 cut(s)	71, 567, 750
PfoI	TCCNGGA	1 cut(s)	940
PleI	GAGTC	3 cut(s)	26, 1056, 1761
PpsI	GAGTC	3 cut(s)	26, 1056, 1761
PshAI	GACNNNNGTC	1 cut(s)	1047
Psp6I	CCWGG	6 cut(s)	651, 835, 940, 1052, 1243, 1301
PspGI	CCWGG	6 cut(s)	651, 835, 940, 1052, 1243, 1301
PspN4I	GGNNCC	3 cut(s)	118, 811, 939
PstI	CTGCAG	1 cut(s)	718
PsuI	RGATCY	2 cut(s)	1092, 1589
PteI	GCGCGC	1 cut(s)	1785
RsaI	GTAC	4 cut(s)	62, 1409, 1499, 1802
RsaNI	GTAC	4 cut(s)	61, 1408, 1498, 1801
RseI	CAYNNNNRTG	1 cut(s)	1235
SaqAI	TTAA	6 cut(s)	243, 306, 851, 1190, 1454, 1821
Sau3AI	GATC	5 cut(s)	154, 472, 1092, 1113, 1589
ScaI	AGTACT	1 cut(s)	1499
SchI	GAGTC	3 cut(s)	26, 1057, 1762
ScrFI	CCNGG	7 cut(s)	653, 837, 942, 1054, 1118, 1245, 1303
SfaNI	GCATC	7 cut(s)	352, 438, 522, 1317, 1561, 1659, 1798
SfcI	CTRYAG	1 cut(s)	714
SfuI	TTCGAA	2 cut(s)	161, 1401
SmiMI	CAYNNNNRTG	1 cut(s)	1235
SmlI	CTYRAG	2 cut(s)	864, 1544
SmoI	CTYRAG	2 cut(s)	864, 1544
SphI	GCATGC	2 cut(s)	1125, 1234
Sse9I	AATT	9 cut(s)	310, 454, 505, 699, 902, 1022, 1131, 1417, 1762
SsiI	CCGC	5 cut(s)	85, 102, 171, 348, 1614
SspMI	CTAG	5 cut(s)	12, 356, 488, 806, 1361
StyD4I	CCNGG	7 cut(s)	651, 835, 940, 1052, 1116, 1243, 1301
StyI	CCWWGG	1 cut(s)	136
TaaI	ACNGT	8 cut(s)	60, 525, 550, 1069, 1269, 1299, 1328, 1747
TaiI	ACGT	2 cut(s)	795, 1851
TaqI	TCGA	4 cut(s)	161, 376, 621, 1401
TaqII	GACCGA	1 cut(s)	221
TasI	AATT	9 cut(s)	310, 454, 505, 699, 902, 1022, 1131, 1417, 1762
TatI	WGTACW	3 cut(s)	60, 1497, 1800
TfiI	GAWTC	3 cut(s)	71, 567, 750
Tru1I	TTAA	6 cut(s)	243, 306, 851, 1190, 1454, 1821
Tru9I	TTAA	6 cut(s)	243, 306, 851, 1190, 1454, 1821
TscAI	CASTG	8 cut(s)	718, 771, 919, 1072, 1176, 1242, 1302, 1873
TseFI	GTSAC	4 cut(s)	685, 762, 1069, 1663
Tsp45I	GTSAC	4 cut(s)	685, 762, 1069, 1663
TspDTI	ATGAA	8 cut(s)	55, 181, 219, 247, 282, 324, 749, 1592
TspGWI	ACGGA	3 cut(s)	1449, 1823, 1841
TspRI	CASTG	8 cut(s)	718, 771, 919, 1072, 1176, 1242, 1302, 1873
XagI	CCTNNNNNAGG	1 cut(s)	1556
XapI	RAATTY	1 cut(s)	902
XceI	RCATGY	3 cut(s)	1125, 1230, 1234
XcmI	CCANNNNNNNNNTGG	1 cut(s)	737
XmiI	GTMKAC	1 cut(s)	1041
XspI	CTAG	5 cut(s)	12, 356, 488, 806, 1361
ZrmI	AGTACT	1 cut(s)	1499
Zsp2I	ATGCAT	3 cut(s)	276, 367, 1232

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.