Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004424

Physical Location & Seq

Genomic Coordinates Forward (+)

10003867 .. 10006664

Gene Structure 2798 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1131800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1914 bp

ATGGCAACTGGAGAAATCAAGTCCCCTGAAGGAGGGACAATGACACCTGTTAGTTTAGATGGAAATCCAGACTCAATGGAGAGAAAGAAACTTGGGCTTTACTTCATCGAATCCGATGACAGGCGGACGGCTTTTGGACAGGGTTATGCTGGAGGAACCACACCAGTTGACATCCATGGAAAACCTATTGCTGATCTTTCGAAAACCGGCGGTTGGTTTGCTGCCTTCTTCATATTCGGAAATGAAATGGCGGAGAGAATGGCGTATTTCGGTCTCTCGGTTAACATGGTGGCCTTCATGTTTTACGTTATGCATAGACCCTTCAGCAGTTCATCAAATGCAGTTAACAATTTTCTCGGGATTTCGCAAGCTTCCTCTGTGCTTGGCGGTTTTCTGGCTGATGCGTATCTTGGTCGATATTGGACAATAGCGATCTTCACAACTATCTATCTTCTGGGTTTGACAGGAATAACCTTATGTGCAACGGTGCACGCTTTGGTGCCAAACCAAGATCAATGCTCCGAGTTAGCCCTCCTTGTGGGCAATTGTGAGCCTGCAAAGCCGTGGCAGATGATTTACCTCTACACGGTTCTGTACATAACTGGATTCGGAGCTGCTGGCATAAGGCCGTGCGTTTCTTCTTTCGGGGCTGACCAGTTCGACGAAAGAAGTAGCGATTACAAGTCTCACCTGGATAGGTTTTTCAACTTCTTTTATCTCTCTGTCACCATCGGGGCGATCATCGCCTTCACTGCAGTAGTCTATGTCCAGATGAAACTTGGTTGGGGATTCGCGTTCGGGTCACTGGCATTGGCTATGGGCATATCAAATATGGTCTTCTTTCTCGGTACCCCTCTATACCGCCATAGACTTCCCGGAGGGAGCCCTTTAACGCGTGTTGCTCAAGTTTTGGTTGCTGCTTACAAGAAGAGGAATGCCGAATTTTCCAGAAGTGAGTTGATAGGCTTGTATGAGGTTCCTGGAAAACAGTCTGCTGTGAAGGGTAGTGGAAAGATAGCTCACACCGACGACTTTAGATGTTTGGACAAAGCAGCTCTGCAGTTAAAGGAAGACAGCGCAGACCCCAGTCCCTGGAGGCTATGCACTGTGACACAAGTAGAGGAAGTGAAGATCTTGTTGAAACTTATTCCAATCCCGGCGTGCACAATAATGCTCAGTGTACTTTTGACAGAATATCTCACTCTCTCAGTGCAGCAGGCATACACTTTAAACACCCATATGGGTAAACTGAAACTCCCTGTAACCTGCATGCCAGTGTTTCCTGGCCTCAGTATCTTTCTCATACTGTCCCTCTACTACTCTACATTTGTCCCGCTGTCCAGGTGCATCACCGGCCATCCCCACGGTGCTTCTCAGCTTCAGAGAGTGGGGATAGGCCTGGCGGTTTCAATCCTATCTGTGGCGTGGGCGGCAATTTTCGAAAGGTACCGAAGGAATTATGCGATACAGCAGGGATACGAGACTAGTTTCTTGAGTGCAATGCCCAACCTAAGTGCATACTGGTTGTTGATCCAGTACTGCCTCATTGGGATAGCTGAGGTGTTTTGCATGGTGGGATTACTAGAATTTCTGTACGAAGAAGCTCCAGATGCAATGAAGAGTATTGGATCTGCTTACGCTGCTCTTGCCGGCGGTTTAGGTTGCTTTGCAGCCAGTATATTGAACAGCATCATCAAATCTATCACCGGAAGCGCAGAGAAGCCGTCTTGGCTGTCCCAGAATATCAACACAGGAAGATTTGATTATTTCTACTGGCTGCTGACTGTTTTGAGCGTGATCAATTTCGGCGTTTTTCTGTATTGTGCTCATCGGTACGAGTACAGGACGGAGCAGGGCGTCCAGATGGAGAAGCAGGCTCTACCCAACATAAAGGAACAACCCCTCAGTGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PTR2

Protein Analysis

638

Amino Acids

70.08

Weight (kDa)

7.85

Isoelectric Point (pI)

34.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFS_1	PF07690	91 - 558	1.1e-07	Major Facilitator Superfamily
PTR2	PF00854	140 - 578	9.4e-79	POT family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004424)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13400
fragaria_vesca	FvH4_4g22520 FvH4_4g22520 FvH4_4g22520 FvH4_4g22520 FvH4_4g22520 FvH4_4g22520 FvH4_4g22520 FvH4_4g22520 FvH4_4g22520
malus_domestica	MD13G1131800.v1.1 MD16G1142100.v1.1
prunus_persica	Prupe.1G213600_v2.0.a1 Prupe.1G213600_v2.0.a1 Prupe.1G213600_v2.0.a1 Prupe.1G213600_v2.0.a1
pyrus_communis	pycom13g11480 pycom16g11260
rosa_chinensis	RchiOBHm_Chr4g0428751
rosa_laevigata	RLG00000007129
rosa_multiflora	Rmu_co8494165.1_g000001 Rmu_sc0010032.1_g000002
rosa_roxburghii	Rroxscaffold_5G00370410
rosa_rugosa	Rorug04G0228000 Rorug04G0228100 Rorug04G0228100 Rorug04G0228200 Rorug04G0228300
rosa_samantha	Rh4AG285400 Rh4BG291400 Rh4DG288500
rosa_wichuraiana	Rw4G024750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1274
Acc65I	GGTACC	2 cut(s)	848, 1446
AccB1I	GGYRCC	3 cut(s)	499, 848, 1446
AccB7I	CCANNNNNTGG	1 cut(s)	1092
AccII	CGCG	2 cut(s)	794, 895
AciI	CCGC	9 cut(s)	124, 210, 251, 387, 862, 1334, 1403, 1430, 1653
AclWI	GGATC	2 cut(s)	1525, 1636
AcoI	YGGCCR	1 cut(s)	1354
AcsI	RAATTY	2 cut(s)	941, 1586
AcuI	CTGAAG	3 cut(s)	48, 307, 1364
AcyI	GRCGYC	1 cut(s)	1857
AfaI	GTAC	8 cut(s)	596, 850, 1182, 1448, 1538, 1595, 1835, 1841
AfiI	CCNNNNNNNGG	8 cut(s)	32, 120, 213, 538, 586, 1092, 1420, 1907
AflIII	ACRYGT	1 cut(s)	893
AgsI	TTSAA	4 cut(s)	706, 1141, 1410, 1684
AhlI	ACTAGT	1 cut(s)	1484
AjnI	CCWGG	6 cut(s)	690, 979, 1091, 1282, 1340, 1398
AluBI	AGCT	7 cut(s)	371, 614, 1019, 1055, 1378, 1556, 1604
AluI	AGCT	7 cut(s)	371, 614, 1019, 1055, 1378, 1556, 1604
Alw21I	GWGCWC	3 cut(s)	492, 1166, 1828
Alw26I	GTCTC	3 cut(s)	278, 690, 1475
Alw44I	GTGCAC	2 cut(s)	488, 1162
AlwI	GGATC	2 cut(s)	1525, 1636
AlwNI	CAGNNNCTG	1 cut(s)	1092
Ama87I	CYCGRG	1 cut(s)	356
AoxI	GGCC	5 cut(s)	291, 626, 1285, 1354, 1396
ApaLI	GTGCAC	2 cut(s)	488, 1162
ApeKI	GCWGC	8 cut(s)	221, 614, 917, 1052, 1213, 1640, 1670, 1777
ApoI	RAATTY	2 cut(s)	941, 1586
Asp718I	GGTACC	2 cut(s)	848, 1446
AspLEI	GCGC	2 cut(s)	1079, 1715
AsuC2I	CCSGG	2 cut(s)	876, 1157
AsuHPI	GGTGA	4 cut(s)	680, 718, 1342, 1696
AsuII	TTCGAA	2 cut(s)	200, 1440
AvaI	CYCGRG	1 cut(s)	356
BaeGI	GKGCMC	2 cut(s)	492, 1166
BanI	GGYRCC	3 cut(s)	499, 848, 1446
BanII	GRGCYC	1 cut(s)	887
BbsI	GAAGAC	2 cut(s)	829, 1077
Bbv12I	GWGCWC	3 cut(s)	492, 1166, 1828
BbvCI	CCTCAGC	1 cut(s)	1557
BbvI	GCAGC	8 cut(s)	208, 601, 904, 1064, 1225, 1627, 1682, 1764
BccI	CCATC	4 cut(s)	53, 737, 1365, 1858
BceAI	ACGGC	4 cut(s)	144, 547, 613, 1708
BciT130I	CCWGG	6 cut(s)	692, 981, 1093, 1284, 1342, 1400
BciVI	GTATCC	1 cut(s)	1469
BclI	TGATCA	1 cut(s)	1797
BcnI	CCSGG	2 cut(s)	876, 1157
BcoDI	GTCTC	3 cut(s)	278, 690, 1475
BcuI	ACTAGT	1 cut(s)	1484
BfaI	CTAG	2 cut(s)	1485, 1583
BfmI	CTRYAG	2 cut(s)	753, 1058
BfuAI	ACCTGC	1 cut(s)	1274
BfuI	GTATCC	1 cut(s)	1469
BglI	GCCNNNNNGGC	1 cut(s)	1729
BglII	AGATCT	1 cut(s)	1131
BisI	GCNGC	9 cut(s)	222, 615, 918, 1053, 1214, 1431, 1641, 1671, 1778
BlsI	GCNGC	9 cut(s)	223, 616, 919, 1054, 1215, 1432, 1642, 1672, 1779
BmcAI	AGTACT	1 cut(s)	1538
Bme1390I	CCNGG	8 cut(s)	692, 876, 981, 1093, 1157, 1284, 1342, 1400
BmeT110I	CYCGRG	1 cut(s)	356
BmiI	GGNNCC	6 cut(s)	157, 501, 850, 884, 978, 1448
BmrFI	CCNGG	8 cut(s)	692, 876, 981, 1093, 1157, 1284, 1342, 1400
BmrI	ACTGGG	1 cut(s)	1080
BmsI	GCATC	4 cut(s)	391, 1356, 1600, 1698
BmuI	ACTGGG	1 cut(s)	1080
BoxI	GACNNNNGTC	1 cut(s)	1086
BpiI	GAAGAC	2 cut(s)	829, 1077
BpmI	CTGGAG	4 cut(s)	30, 171, 1114, 1590
Bpu10I	CCTNAGC	1 cut(s)	1557
Bpu14I	TTCGAA	2 cut(s)	200, 1440
BpuEI	CTTGAG	2 cut(s)	888, 1513
BpuMI	CCSGG	2 cut(s)	876, 1157
BsaBI	GATNNNNATC	2 cut(s)	63, 405
BsaHI	GRCGYC	1 cut(s)	1857
BsaI	GGTCTC	1 cut(s)	278
BsaJI	CCNNGG	4 cut(s)	175, 563, 1091, 1363
BsaWI	WCCGGW	1 cut(s)	1706
BsaXI	ACNNNNNCTCC	2 cut(s)	1239, 1269
Bsc4I	CCNNNNNNNGG	8 cut(s)	32, 120, 213, 538, 586, 1092, 1420, 1907
Bse118I	RCCGGY	3 cut(s)	206, 1352, 1649
Bse3DI	GCAATG	2 cut(s)	1506, 1620
Bse8I	GATNNNNATC	2 cut(s)	63, 405
BseBI	CCWGG	6 cut(s)	692, 981, 1093, 1284, 1342, 1400
BseDI	CCNNGG	4 cut(s)	175, 563, 1091, 1363
BseGI	GGATG	2 cut(s)	171, 1357
BseJI	GATNNNNATC	2 cut(s)	63, 405
BseLI	CCNNNNNNNGG	8 cut(s)	32, 120, 213, 538, 586, 1092, 1420, 1907
BseMI	GCAATG	2 cut(s)	1506, 1620
BseMII	CTCAG	5 cut(s)	1189, 1221, 1303, 1388, 1548
BseSI	GKGCMC	2 cut(s)	492, 1166
BseXI	GCAGC	8 cut(s)	208, 601, 904, 1064, 1225, 1627, 1682, 1764
BsgI	GTGCAG	1 cut(s)	1232
Bsh1236I	CGCG	2 cut(s)	794, 895
BshFI	GGCC	5 cut(s)	293, 628, 1287, 1356, 1398
BshNI	GGYRCC	3 cut(s)	499, 848, 1446
BsiHKAI	GWGCWC	3 cut(s)	492, 1166, 1828
BsiHKCI	CYCGRG	1 cut(s)	356
BsiSI	CCGG	6 cut(s)	207, 876, 1157, 1353, 1650, 1707
BslFI	GGGAC	6 cut(s)	7, 49, 1074, 1294, 1316, 1720
BslI	CCNNNNNNNGG	8 cut(s)	32, 120, 213, 538, 586, 1092, 1420, 1907
BsmAI	GTCTC	3 cut(s)	278, 690, 1475
BsmFI	GGGAC	6 cut(s)	7, 49, 1074, 1294, 1316, 1720
BsmI	GAATGC	1 cut(s)	940
BsnI	GGCC	5 cut(s)	293, 628, 1287, 1356, 1398
Bso31I	GGTCTC	1 cut(s)	278
BsoBI	CYCGRG	1 cut(s)	356
Bsp119I	TTCGAA	2 cut(s)	200, 1440
Bsp1286I	GDGCHC	4 cut(s)	492, 887, 1166, 1828
Bsp1407I	TGTACA	1 cut(s)	594
Bsp143I	GATC	8 cut(s)	193, 432, 511, 738, 1131, 1530, 1628, 1797
Bsp19I	CCATGG	1 cut(s)	175
BspACI	CCGC	9 cut(s)	124, 210, 251, 387, 862, 1334, 1403, 1430, 1653
BspANI	GGCC	5 cut(s)	293, 628, 1287, 1356, 1398
BspCNI	CTCAG	5 cut(s)	1188, 1220, 1302, 1387, 1549
BspFNI	CGCG	2 cut(s)	794, 895
BspLI	GGNNCC	6 cut(s)	157, 501, 850, 884, 978, 1448
BspMAI	CTGCAG	2 cut(s)	757, 1062
BspMI	ACCTGC	1 cut(s)	1274
BspPI	GGATC	2 cut(s)	1525, 1636
BspT104I	TTCGAA	2 cut(s)	200, 1440
BspT107I	GGYRCC	3 cut(s)	499, 848, 1446
BspTNI	GGTCTC	1 cut(s)	278
BsrDI	GCAATG	2 cut(s)	1506, 1620
BsrFI	RCCGGY	3 cut(s)	206, 1352, 1649
BsrGI	TGTACA	1 cut(s)	594
BssAI	RCCGGY	3 cut(s)	206, 1352, 1649
BssECI	CCNNGG	4 cut(s)	175, 563, 1091, 1363
BssMI	GATC	8 cut(s)	193, 432, 511, 738, 1131, 1530, 1628, 1797
BssNI	GRCGYC	1 cut(s)	1857
BssT1I	CCWWGG	1 cut(s)	175
Bst2UI	CCWGG	6 cut(s)	692, 981, 1093, 1284, 1342, 1400
Bst4CI	ACNGT	7 cut(s)	487, 589, 990, 1108, 1308, 1367, 1786
Bst6I	CTCTTC	2 cut(s)	923, 1613
BstACI	GRCGYC	1 cut(s)	1857
BstAUI	TGTACA	1 cut(s)	594
BstBI	TTCGAA	2 cut(s)	200, 1440
BstC8I	GCNNGC	9 cut(s)	369, 492, 555, 619, 1162, 1218, 1271, 1651, 1875
BstDEI	CTNAG	7 cut(s)	1175, 1207, 1289, 1374, 1511, 1557, 1904
BstDSI	CCRYGG	3 cut(s)	175, 563, 1363
BstENI	CCTNNNNNAGG	1 cut(s)	30
BstF5I	GGATG	2 cut(s)	171, 1357
BstFNI	CGCG	2 cut(s)	794, 895
BstHHI	GCGC	2 cut(s)	1079, 1715
BstKTI	GATC	8 cut(s)	196, 435, 514, 741, 1134, 1533, 1631, 1800
BstMAI	GTCTC	3 cut(s)	278, 690, 1475
BstMBI	GATC	8 cut(s)	193, 432, 511, 738, 1131, 1530, 1628, 1797
BstMWI	GCNNNNNNNGC	9 cut(s)	559, 743, 752, 1353, 1430, 1610, 1640, 1646, 1729
BstNI	CCWGG	6 cut(s)	692, 981, 1093, 1284, 1342, 1400
BstNSI	RCATGY	1 cut(s)	1273
BstPAI	GACNNNNGTC	1 cut(s)	1086
BstSCI	CCNGG	8 cut(s)	690, 874, 979, 1091, 1155, 1282, 1340, 1398
BstSFI	CTRYAG	2 cut(s)	753, 1058
BstSLI	GKGCMC	2 cut(s)	492, 1166
BstUI	CGCG	2 cut(s)	794, 895
BstV1I	GCAGC	8 cut(s)	208, 601, 904, 1064, 1225, 1627, 1682, 1764
BstV2I	GAAGAC	2 cut(s)	829, 1077
BstX2I	RGATCY	2 cut(s)	1131, 1628
BstYI	RGATCY	2 cut(s)	1131, 1628
BsuI	GTATCC	1 cut(s)	1469
BsuRI	GGCC	5 cut(s)	293, 628, 1287, 1356, 1398
BtgI	CCRYGG	3 cut(s)	175, 563, 1363
BtgZI	GCGATG	1 cut(s)	727
BtsCI	GGATG	2 cut(s)	171, 1357
BtsI	GCAGTG	1 cut(s)	750
BtsIMutI	CAGTG	7 cut(s)	750, 803, 1104, 1183, 1215, 1281, 1912
BveI	ACCTGC	1 cut(s)	1274
Cac8I	GCNNGC	9 cut(s)	369, 492, 555, 619, 1162, 1218, 1271, 1651, 1875
CaiI	CAGNNNCTG	1 cut(s)	1092
CfoI	GCGC	2 cut(s)	1079, 1715
Cfr10I	RCCGGY	3 cut(s)	206, 1352, 1649
CseI	GACGC	1 cut(s)	1846
Csp6I	GTAC	8 cut(s)	595, 849, 1181, 1447, 1537, 1594, 1834, 1840
CspCI	CAANNNNNGTGG	2 cut(s)	148, 183
CviAII	CATG	5 cut(s)	176, 286, 298, 1270, 1570
CviQI	GTAC	8 cut(s)	595, 849, 1181, 1447, 1537, 1594, 1834, 1840
DdeI	CTNAG	7 cut(s)	1175, 1207, 1289, 1374, 1511, 1557, 1904
DpnI	GATC	8 cut(s)	195, 434, 513, 740, 1133, 1532, 1630, 1799
DpnII	GATC	8 cut(s)	193, 432, 511, 738, 1131, 1530, 1628, 1797
DraI	TTTAAA	1 cut(s)	1230
EaeI	YGGCCR	1 cut(s)	1354
Eam1104I	CTCTTC	2 cut(s)	923, 1613
EarI	CTCTTC	2 cut(s)	923, 1613
EciI	GGCGGA	2 cut(s)	139, 266
Eco130I	CCWWGG	1 cut(s)	175
Eco147I	AGGCCT	1 cut(s)	1398
Eco24I	GRGCYC	1 cut(s)	887
Eco31I	GGTCTC	1 cut(s)	278
Eco57I	CTGAAG	3 cut(s)	48, 307, 1364
Eco88I	CYCGRG	1 cut(s)	356
EcoNI	CCTNNNNNAGG	1 cut(s)	30
EcoRII	CCWGG	6 cut(s)	690, 979, 1091, 1282, 1340, 1398
EcoT14I	CCWWGG	1 cut(s)	175
EcoT22I	ATGCAT	1 cut(s)	315
EcoT38I	GRGCYC	1 cut(s)	887
ErhI	CCWWGG	1 cut(s)	175
FaeI	CATG	5 cut(s)	179, 289, 301, 1273, 1573
FaqI	GGGAC	6 cut(s)	7, 49, 1074, 1294, 1316, 1720
FatI	CATG	5 cut(s)	175, 285, 297, 1269, 1569
FauI	CCCGC	1 cut(s)	1341
FauNDI	CATATG	1 cut(s)	1239
FbaI	TGATCA	1 cut(s)	1797
Fnu4HI	GCNGC	9 cut(s)	222, 615, 918, 1053, 1214, 1431, 1641, 1671, 1778
FokI	GGATG	2 cut(s)	158, 1344
FriOI	GRGCYC	1 cut(s)	887
Fsp4HI	GCNGC	9 cut(s)	222, 615, 918, 1053, 1214, 1431, 1641, 1671, 1778
FspBI	CTAG	2 cut(s)	1485, 1583
GlaI	GCGC	2 cut(s)	1078, 1714
GluI	GCNGC	9 cut(s)	222, 615, 918, 1053, 1214, 1431, 1641, 1671, 1778
GsuI	CTGGAG	4 cut(s)	30, 171, 1114, 1590
HaeIII	GGCC	5 cut(s)	293, 628, 1287, 1356, 1398
HapII	CCGG	6 cut(s)	207, 876, 1157, 1353, 1650, 1707
HgaI	GACGC	1 cut(s)	1846
HhaI	GCGC	2 cut(s)	1079, 1715
Hin1I	GRCGYC	1 cut(s)	1857
Hin1II	CATG	5 cut(s)	179, 289, 301, 1273, 1573
Hin6I	GCGC	2 cut(s)	1077, 1713
HinP1I	GCGC	2 cut(s)	1077, 1713
HincII	GTYRAC	3 cut(s)	169, 283, 346
HindII	GTYRAC	3 cut(s)	169, 283, 346
HindIII	AAGCTT	1 cut(s)	369
HinfI	GANTC	4 cut(s)	71, 110, 606, 789
HpaI	GTTAAC	2 cut(s)	283, 346
HpaII	CCGG	6 cut(s)	207, 876, 1157, 1353, 1650, 1707
HphI	GGTGA	4 cut(s)	680, 718, 1342, 1696
Hpy166II	GTNNAC	7 cut(s)	169, 283, 346, 490, 1164, 1181, 1247
Hpy188I	TCNGA	5 cut(s)	115, 239, 523, 611, 1383
Hpy188III	TCNNGA	7 cut(s)	68, 358, 769, 948, 1492, 1607, 1861
Hpy8I	GTNNAC	7 cut(s)	169, 283, 346, 490, 1164, 1181, 1247
Hpy99I	CGWCG	2 cut(s)	665, 1031
HpyAV	CCTTC	7 cut(s)	23, 235, 304, 331, 757, 994, 1446
HpyCH4III	ACNGT	7 cut(s)	487, 589, 990, 1108, 1308, 1367, 1786
HpyCH4IV	ACGT	1 cut(s)	306
HpyF10VI	GCNNNNNNNGC	9 cut(s)	559, 743, 752, 1353, 1430, 1610, 1640, 1646, 1729
HpyF3I	CTNAG	7 cut(s)	1175, 1207, 1289, 1374, 1511, 1557, 1904
HpySE526I	ACGT	1 cut(s)	306
Hsp92I	GRCGYC	1 cut(s)	1857
Hsp92II	CATG	5 cut(s)	179, 289, 301, 1273, 1573
HspAI	GCGC	2 cut(s)	1077, 1713
KpnI	GGTACC	2 cut(s)	852, 1450
KroI	GCCGGC	1 cut(s)	1649
KroNI	GCCGGC	1 cut(s)	1651
Ksp22I	TGATCA	1 cut(s)	1797
KspAI	GTTAAC	2 cut(s)	283, 346
Kzo9I	GATC	8 cut(s)	193, 432, 511, 738, 1131, 1530, 1628, 1797
LmnI	GCTCC	5 cut(s)	524, 611, 882, 1609, 1849
Lsp1109I	GCAGC	8 cut(s)	208, 601, 904, 1064, 1225, 1627, 1682, 1764
LweI	GCATC	4 cut(s)	391, 1356, 1600, 1698
MaeI	CTAG	2 cut(s)	1485, 1583
MaeII	ACGT	1 cut(s)	306
MaeIII	GTNAC	4 cut(s)	724, 801, 1108, 1261
MalI	GATC	8 cut(s)	195, 434, 513, 740, 1133, 1532, 1630, 1799
MboI	GATC	8 cut(s)	193, 432, 511, 738, 1131, 1530, 1628, 1797
MfeI	CAATTG	1 cut(s)	544
MflI	RGATCY	2 cut(s)	1131, 1628
MhlI	GDGCHC	4 cut(s)	492, 887, 1166, 1828
MluCI	AATT	7 cut(s)	349, 544, 941, 1434, 1456, 1586, 1801
MluI	ACGCGT	1 cut(s)	893
MlyI	GAGTC	1 cut(s)	65
Mph1103I	ATGCAT	1 cut(s)	315
MroNI	GCCGGC	1 cut(s)	1649
MseI	TTAA	5 cut(s)	282, 345, 890, 1064, 1229
MslI	CAYNNNNRTG	3 cut(s)	1169, 1238, 1274
MspA1I	CMGCKG	1 cut(s)	1336
MspI	CCGG	6 cut(s)	207, 876, 1157, 1353, 1650, 1707
MspR9I	CCNGG	8 cut(s)	692, 876, 981, 1093, 1157, 1284, 1342, 1400
MunI	CAATTG	1 cut(s)	544
Mva1269I	GAATGC	1 cut(s)	940
MvaI	CCWGG	6 cut(s)	692, 981, 1093, 1284, 1342, 1400
MvnI	CGCG	2 cut(s)	794, 895
MwoI	GCNNNNNNNGC	9 cut(s)	559, 743, 752, 1353, 1430, 1610, 1640, 1646, 1729
NaeI	GCCGGC	1 cut(s)	1651
NciI	CCSGG	2 cut(s)	876, 1157
NcoI	CCATGG	1 cut(s)	175
NdeI	CATATG	1 cut(s)	1239
NdeII	GATC	8 cut(s)	193, 432, 511, 738, 1131, 1530, 1628, 1797
NgoMIV	GCCGGC	1 cut(s)	1649
NlaIII	CATG	5 cut(s)	179, 289, 301, 1273, 1573
NlaIV	GGNNCC	6 cut(s)	157, 501, 850, 884, 978, 1448
NmuCI	GTSAC	3 cut(s)	724, 801, 1108
NsiI	ATGCAT	1 cut(s)	315
NspI	RCATGY	1 cut(s)	1273
NspV	TTCGAA	2 cut(s)	200, 1440
PaeI	GCATGC	1 cut(s)	1273
PceI	AGGCCT	1 cut(s)	1398
PctI	GAATGC	1 cut(s)	940
PdiI	GCCGGC	1 cut(s)	1651
PfeI	GAWTC	3 cut(s)	110, 606, 789
PflMI	CCANNNNNTGG	1 cut(s)	1092
PfoI	TCCNGGA	2 cut(s)	874, 979
PkrI	GCNGC	9 cut(s)	223, 616, 919, 1054, 1215, 1432, 1642, 1672, 1779
PleI	GAGTC	1 cut(s)	65
PpsI	GAGTC	1 cut(s)	65
PshAI	GACNNNNGTC	1 cut(s)	1086
Psp6I	CCWGG	6 cut(s)	690, 979, 1091, 1282, 1340, 1398
PspGI	CCWGG	6 cut(s)	690, 979, 1091, 1282, 1340, 1398
PspN4I	GGNNCC	6 cut(s)	157, 501, 850, 884, 978, 1448
PstI	CTGCAG	2 cut(s)	757, 1062
PstNI	CAGNNNCTG	1 cut(s)	1092
PsuI	RGATCY	2 cut(s)	1131, 1628
RsaI	GTAC	8 cut(s)	596, 850, 1182, 1448, 1538, 1595, 1835, 1841
RsaNI	GTAC	8 cut(s)	595, 849, 1181, 1447, 1537, 1594, 1834, 1840
RseI	CAYNNNNRTG	3 cut(s)	1169, 1238, 1274
SaqAI	TTAA	5 cut(s)	282, 345, 890, 1064, 1229
SatI	GCNGC	9 cut(s)	222, 615, 918, 1053, 1214, 1431, 1641, 1671, 1778
Sau3AI	GATC	8 cut(s)	193, 432, 511, 738, 1131, 1530, 1628, 1797
ScaI	AGTACT	1 cut(s)	1538
SchI	GAGTC	1 cut(s)	65
ScrFI	CCNGG	8 cut(s)	692, 876, 981, 1093, 1157, 1284, 1342, 1400
SduI	GDGCHC	4 cut(s)	492, 887, 1166, 1828
SfaNI	GCATC	4 cut(s)	391, 1356, 1600, 1698
SfcI	CTRYAG	2 cut(s)	753, 1058
SfuI	TTCGAA	2 cut(s)	200, 1440
SmiMI	CAYNNNNRTG	3 cut(s)	1169, 1238, 1274
SmlI	CTYRAG	2 cut(s)	903, 1492
SmoI	CTYRAG	2 cut(s)	903, 1492
SpeI	ACTAGT	1 cut(s)	1484
SphI	GCATGC	1 cut(s)	1273
Sse9I	AATT	7 cut(s)	349, 544, 941, 1434, 1456, 1586, 1801
SseBI	AGGCCT	1 cut(s)	1398
SsiI	CCGC	9 cut(s)	124, 210, 251, 387, 862, 1334, 1403, 1430, 1653
SspMI	CTAG	2 cut(s)	1485, 1583
StuI	AGGCCT	1 cut(s)	1398
StyD4I	CCNGG	8 cut(s)	690, 874, 979, 1091, 1155, 1282, 1340, 1398
StyI	CCWWGG	1 cut(s)	175
TaaI	ACNGT	7 cut(s)	487, 589, 990, 1108, 1308, 1367, 1786
TaiI	ACGT	1 cut(s)	309
TaqI	TCGA	5 cut(s)	108, 200, 415, 660, 1440
TaqII	GACCGA	1 cut(s)	260
TasI	AATT	7 cut(s)	349, 544, 941, 1434, 1456, 1586, 1801
TatI	WGTACW	4 cut(s)	594, 1180, 1536, 1839
TauI	GCSGC	1 cut(s)	1433
TfiI	GAWTC	3 cut(s)	110, 606, 789
Tru1I	TTAA	5 cut(s)	282, 345, 890, 1064, 1229
Tru9I	TTAA	5 cut(s)	282, 345, 890, 1064, 1229
TscAI	CASTG	7 cut(s)	757, 810, 1111, 1183, 1215, 1281, 1912
TseFI	GTSAC	3 cut(s)	724, 801, 1108
TseI	GCWGC	8 cut(s)	221, 614, 917, 1052, 1213, 1640, 1670, 1777
Tsp45I	GTSAC	3 cut(s)	724, 801, 1108
TspDTI	ATGAA	7 cut(s)	94, 220, 258, 286, 321, 788, 1631
TspGWI	ACGGA	1 cut(s)	1862
TspRI	CASTG	7 cut(s)	757, 810, 1111, 1183, 1215, 1281, 1912
Van91I	CCANNNNNTGG	1 cut(s)	1092
VneI	GTGCAC	2 cut(s)	488, 1162
XagI	CCTNNNNNAGG	1 cut(s)	30
XapI	RAATTY	2 cut(s)	941, 1586
XceI	RCATGY	1 cut(s)	1273
XcmI	CCANNNNNNNNNTGG	1 cut(s)	776
XspI	CTAG	2 cut(s)	1485, 1583
ZrmI	AGTACT	1 cut(s)	1538
Zsp2I	ATGCAT	1 cut(s)	315

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.