FvH4_4g23320

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016314
Physical Location & Seq
Reverse (-)
25831986 .. 25835319
3334 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23320.t2

Sequence Viewer

Length: 1353 bp
ATGGATTGTTGGTCTCCTTCTCTTGCTGTTGACGACGAGTTTCAGAAGCTGGCTTTGAGAGTGAACCCTCCAAGGGTTACAGTTGATAATGGCACAAGCAGGAAAGCGACTCTGATTAAGGTAGATAGTGCAAATAAGCGTGGGAGTTTGCTGGAGGTGGTTCAGGTTTTGAATGATTTGAATCTCATTGTAAGAAGAGCTTACATTTCTTCAGATGGGGAGTGGTTTATGGATGTGTTTCATGTCACTGATCAACAGGGGAATAAGCTTTATGATGATGGAATAGCTGAGTGCATTCAACAGTCTCTGGGACCAAGAGCGCGGAGCTTCCGTGCCTTGAGAAGTTCTGTGGGTGTCCAAGCTGCCTCAGAACACACTACTATCGAATTGACTGGAAGGGACAGAGCAGGCTTGCTTTCGGAGGTTTTTGCTGTTCTTGCTGAACTCAAATGTAATGTAGTTGCCGGAGAAGTGTGGACCCATAATTCAAGAATGGCATCAGTTGTTTACATCACAGATGAGGAAACTGGGAAGCCTATTGATGATCCTTTGCGTCTTTCTGAAATCAAAGAGTTTCTCCTTAATGTGCTCAAGGCAGACAAAGATATGCGCACTGCCAACACTGCTGTATCTGTAGGCTCTACACATAAGGAGAGGAGGCTTCACCAGATGATGTATGCAGACCGTGATTATGACCTGCACAGTGCAGATTCTGGATCAGCCAGTGATCGAACCAAACCCTTTGTAACTGTTGAAAATTGTGCAGATAAAGGATATACTGTTGTGAACTTGAGGTGTCCGGATCGTCCTAAGTTGCTCTTCGATACAGTATGCACCTTAACAGATATGCAATATGTGGTATATCATGCAACTGTGATTGCTGAAGGACCAGAGGCTTATCAGGAATATTATATAAGACACACAGATGGCTGCCCTATAAGTTCTGAAGGAGAGAGGCAGAGGGTAATCCATTGCTTGGAGGCTGCTATTCGAAGGCGAACTTCTGAGGGTATAAGACTAGAACTCTGTGGTGATGACAGAGCCGGTCTTCTTTCTGATGTGACTCGCATATTCAGAGAAAACAGCCTTTCAGTCACCCGAGCTGAGGTCATGACAAGGGGATCCCAAGCTGTGAACGTTTTCTACGTGACTGGCGCATCTGGAAATCCAGTGACGAGTGAGACTATTGAGGCAGTTCGGCAAGAGATTGGTTTGACAATACTACGCGTAGAAGAAGACATGTACTCAAAATCTCCACCACAGGAGAGTGGGAGATTCTCTCTAGGAAATCTCTTTCGGACAAGATCAGAGAAGTTCCTTTACAACTTGGGTTTGATCAAGTCGTGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

451

Amino Acids

50.25

Weight (kDa)

5.72

Isoelectric Point (pI)

39.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 50 - 100 8.6e-08 ACT domain
ACT PF01842 264 - 323 1.5e-07 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016314)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23320 FvH4_4g23320
malus_domestica MD13G1123000.v1.1 MD16G1123700.v1.1
prunus_persica Prupe.1G221900_v2.0.a1
pyrus_communis pycom13g10740 pycom16g10570
rosa_chinensis RchiOBHm_Chr4g0429671
rosa_laevigata RLG00000007053
rosa_multiflora Rmu_sc0010349.1_g000004
rosa_roxburghii Rroxscaffold_5G00371350
rosa_rugosa Rorug04G0237600
rosa_samantha Rh4AG293700 Rh4DG296600
rosa_wichuraiana Rw4G025420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 611
Acc36I ACCTGC 1 cut(s) 705
AccB7I CCANNNNNTGG 1 cut(s) 976
AccII CGCG 2 cut(s) 322, 1227
AccIII TCCGGA 1 cut(s) 799
AciI CCGC 1 cut(s) 322
AclI AACGTT 1 cut(s) 1137
AclWI GGATC 5 cut(s) 539, 724, 810, 1116, 1129
AcuI CTGAAG 3 cut(s) 195, 903, 966
AfaI GTAC 1 cut(s) 1244
AfiI CCNNNNNNNGG 3 cut(s) 73, 976, 1105
AflIII ACRYGT 2 cut(s) 1225, 1239
AgsI TTSAA 5 cut(s) 172, 181, 299, 489, 755
AluBI AGCT 8 cut(s) 49, 200, 268, 287, 327, 362, 1103, 1130
AluI AGCT 8 cut(s) 49, 200, 268, 287, 327, 362, 1103, 1130
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 3 cut(s) 18, 309, 1175
AlwI GGATC 5 cut(s) 539, 724, 810, 1116, 1129
AlwNI CAGNNNCTG 3 cut(s) 49, 307, 713
Ama87I CYCGRG 1 cut(s) 1098
Aor13HI TCCGGA 1 cut(s) 799
ApeKI GCWGC 3 cut(s) 362, 930, 983
Asp700I GAANNNNTTC 1 cut(s) 1139
AspLEI GCGC 3 cut(s) 322, 612, 1157
AspS9I GGNCC 3 cut(s) 311, 477, 887
AsuHPI GGTGA 3 cut(s) 656, 1043, 1087
AsuII TTCGAA 1 cut(s) 991
AvaI CYCGRG 1 cut(s) 1098
AvaII GGWCC 3 cut(s) 311, 477, 887
BaeI ACNNNNGTAYC 2 cut(s) 612, 645
BamHI GGATCC 1 cut(s) 1121
BarI GAAGNNNNNNTAC 2 cut(s) 1304, 1336
BbsI GAAGAC 2 cut(s) 1040, 1242
Bbv12I GWGCWC 1 cut(s) 591
BbvCI CCTCAGC 1 cut(s) 1104
BbvI GCAGC 3 cut(s) 349, 917, 970
BccI CCATC 3 cut(s) 209, 272, 920
BclI TGATCA 2 cut(s) 250, 1335
BcoDI GTCTC 3 cut(s) 18, 309, 1175
BfaI CTAG 2 cut(s) 1019, 1283
BfmI CTRYAG 1 cut(s) 633
BfuAI ACCTGC 1 cut(s) 705
BisI GCNGC 3 cut(s) 363, 931, 984
BlsI GCNGC 3 cut(s) 364, 932, 985
Bme18I GGWCC 3 cut(s) 311, 477, 887
BmeT110I CYCGRG 1 cut(s) 1098
BmgT120I GGNCC 3 cut(s) 311, 477, 887
BmiI GGNNCC 3 cut(s) 312, 479, 1123
BmrI ACTGGG 1 cut(s) 537
BmsI GCATC 2 cut(s) 506, 1166
BmuI ACTGGG 1 cut(s) 537
BpiI GAAGAC 2 cut(s) 1040, 1242
BplI GAGNNNNNCTC 2 cut(s) 1264, 1296
BpmI CTGGAG 1 cut(s) 173
Bpu10I CCTNAGC 1 cut(s) 1104
Bpu14I TTCGAA 1 cut(s) 991
BpuEI CTTGAG 3 cut(s) 358, 575, 811
BsaAI YACGTR 1 cut(s) 1147
BsaBI GATNNNNATC 1 cut(s) 180
BsaI GGTCTC 1 cut(s) 18
BsaJI CCNNGG 1 cut(s) 71
BsaWI WCCGGW 1 cut(s) 799
BsaXI ACNNNNNCTCC 2 cut(s) 316, 346
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 976, 1105
Bse118I RCCGGY 1 cut(s) 1043
Bse1I ACTGG 5 cut(s) 397, 532, 723, 1156, 1169
Bse3DI GCAATG 1 cut(s) 970
Bse8I GATNNNNATC 1 cut(s) 180
BseAI TCCGGA 1 cut(s) 799
BseDI CCNNGG 1 cut(s) 71
BseGI GGATG 1 cut(s) 238
BseJI GATNNNNATC 1 cut(s) 180
BseLI CCNNNNNNNGG 3 cut(s) 73, 976, 1105
BseMI GCAATG 1 cut(s) 970
BseMII CTCAG 4 cut(s) 279, 381, 996, 1095
BseNI ACTGG 5 cut(s) 397, 532, 723, 1156, 1169
BseRI GAGGAG 1 cut(s) 670
BseXI GCAGC 3 cut(s) 349, 917, 970
BsgI GTGCAG 3 cut(s) 683, 726, 783
Bsh1236I CGCG 2 cut(s) 322, 1227
BsiHKAI GWGCWC 1 cut(s) 591
BsiHKCI CYCGRG 1 cut(s) 1098
BsiSI CCGG 3 cut(s) 465, 800, 1044
BslFI GGGAC 2 cut(s) 324, 413
BslI CCNNNNNNNGG 3 cut(s) 73, 976, 1105
BsmAI GTCTC 3 cut(s) 18, 309, 1175
BsmFI GGGAC 2 cut(s) 324, 413
BsmI GAATGC 1 cut(s) 294
Bso31I GGTCTC 1 cut(s) 18
BsoBI CYCGRG 1 cut(s) 1098
Bsp119I TTCGAA 1 cut(s) 991
Bsp1286I GDGCHC 1 cut(s) 591
Bsp13I TCCGGA 1 cut(s) 799
Bsp143I GATC 8 cut(s) 250, 544, 716, 727, 802, 1121, 1304, 1335
BspACI CCGC 1 cut(s) 322
BspCNI CTCAG 4 cut(s) 280, 380, 997, 1096
BspEI TCCGGA 1 cut(s) 799
BspFNI CGCG 2 cut(s) 322, 1227
BspHI TCATGA 1 cut(s) 1110
BspLI GGNNCC 3 cut(s) 312, 479, 1123
BspMI ACCTGC 1 cut(s) 705
BspPI GGATC 5 cut(s) 539, 724, 810, 1116, 1129
BspQI GCTCTTC 2 cut(s) 190, 824
BspT104I TTCGAA 1 cut(s) 991
BspTNI GGTCTC 1 cut(s) 18
BsrDI GCAATG 1 cut(s) 970
BsrFI RCCGGY 1 cut(s) 1043
BsrI ACTGG 5 cut(s) 397, 532, 723, 1156, 1169
BssAI RCCGGY 1 cut(s) 1043
BssECI CCNNGG 1 cut(s) 71
BssMI GATC 8 cut(s) 250, 544, 716, 727, 802, 1121, 1304, 1335
BssT1I CCWWGG 1 cut(s) 71
Bst4CI ACNGT 8 cut(s) 82, 303, 686, 704, 751, 781, 829, 874
Bst6I CTCTTC 2 cut(s) 190, 824
BstBAI YACGTR 1 cut(s) 1147
BstBI TTCGAA 1 cut(s) 991
BstC8I GCNNGC 3 cut(s) 51, 409, 413
BstDEI CTNAG 5 cut(s) 288, 367, 810, 1005, 1104
BstF5I GGATG 1 cut(s) 238
BstFNI CGCG 2 cut(s) 322, 1227
BstHHI GCGC 3 cut(s) 322, 612, 1157
BstKTI GATC 8 cut(s) 253, 547, 719, 730, 805, 1124, 1307, 1338
BstMAI GTCTC 3 cut(s) 18, 309, 1175
BstMBI GATC 8 cut(s) 250, 544, 716, 727, 802, 1121, 1304, 1335
BstMWI GCNNNNNNNGC 2 cut(s) 437, 623
BstNSI RCATGY 1 cut(s) 1243
BstSFI CTRYAG 1 cut(s) 633
BstUI CGCG 2 cut(s) 322, 1227
BstV1I GCAGC 3 cut(s) 349, 917, 970
BstV2I GAAGAC 2 cut(s) 1040, 1242
BstX2I RGATCY 1 cut(s) 1121
BstYI RGATCY 1 cut(s) 1121
BtsCI GGATG 1 cut(s) 238
BtsI GCAGTG 2 cut(s) 612, 621
BtsIMutI CAGTG 6 cut(s) 246, 612, 621, 709, 730, 1176
BveI ACCTGC 1 cut(s) 705
Cac8I GCNNGC 3 cut(s) 51, 409, 413
CaiI CAGNNNCTG 3 cut(s) 49, 307, 713
CciI TCATGA 1 cut(s) 1110
CfoI GCGC 3 cut(s) 322, 612, 1157
Cfr10I RCCGGY 1 cut(s) 1043
Cfr13I GGNCC 3 cut(s) 311, 477, 887
CseI GACGC 1 cut(s) 542
Csp6I GTAC 1 cut(s) 1243
CviAII CATG 4 cut(s) 242, 866, 1111, 1240
CviQI GTAC 1 cut(s) 1243
DdeI CTNAG 5 cut(s) 288, 367, 810, 1005, 1104
DpnI GATC 8 cut(s) 252, 546, 718, 729, 804, 1123, 1306, 1337
DpnII GATC 8 cut(s) 250, 544, 716, 727, 802, 1121, 1304, 1335
Eam1104I CTCTTC 2 cut(s) 190, 824
EarI CTCTTC 2 cut(s) 190, 824
Eco130I CCWWGG 1 cut(s) 71
Eco31I GGTCTC 1 cut(s) 18
Eco47I GGWCC 3 cut(s) 311, 477, 887
Eco57I CTGAAG 3 cut(s) 195, 903, 966
Eco88I CYCGRG 1 cut(s) 1098
EcoT14I CCWWGG 1 cut(s) 71
ErhI CCWWGG 1 cut(s) 71
FaeI CATG 4 cut(s) 245, 869, 1114, 1243
FalI AAGNNNNNCTT 6 cut(s) 184, 216, 803, 835, 985, 1017
FaqI GGGAC 2 cut(s) 324, 413
FatI CATG 4 cut(s) 241, 865, 1110, 1239
FbaI TGATCA 2 cut(s) 250, 1335
Fnu4HI GCNGC 3 cut(s) 363, 931, 984
FokI GGATG 1 cut(s) 245
Fsp4HI GCNGC 3 cut(s) 363, 931, 984
FspAI RTGCGCAY 1 cut(s) 611
FspBI CTAG 2 cut(s) 1019, 1283
FspI TGCGCA 1 cut(s) 611
GlaI GCGC 3 cut(s) 321, 611, 1156
GluI GCNGC 3 cut(s) 363, 931, 984
GsuI CTGGAG 1 cut(s) 173
HapII CCGG 3 cut(s) 465, 800, 1044
HgaI GACGC 1 cut(s) 542
HhaI GCGC 3 cut(s) 322, 612, 1157
Hin1II CATG 4 cut(s) 245, 869, 1114, 1243
Hin6I GCGC 3 cut(s) 320, 610, 1155
HinP1I GCGC 3 cut(s) 320, 610, 1155
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HindIII AAGCTT 1 cut(s) 266
HinfI GANTC 5 cut(s) 109, 181, 710, 1063, 1275
HpaII CCGG 3 cut(s) 465, 800, 1044
HphI GGTGA 3 cut(s) 656, 1043, 1087
Hpy166II GTNNAC 6 cut(s) 31, 64, 477, 508, 787, 1135
Hpy188III TCNNGA 7 cut(s) 489, 714, 800, 902, 1111, 1161, 1350
Hpy8I GTNNAC 6 cut(s) 31, 64, 477, 508, 787, 1135
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 5 cut(s) 27, 390, 878, 941, 987
HpyCH4III ACNGT 8 cut(s) 82, 303, 686, 704, 751, 781, 829, 874
HpyCH4IV ACGT 2 cut(s) 1137, 1146
HpyCH4V TGCA 9 cut(s) 131, 294, 680, 700, 707, 764, 834, 850, 869
HpyF10VI GCNNNNNNNGC 2 cut(s) 437, 623
HpyF3I CTNAG 5 cut(s) 288, 367, 810, 1005, 1104
HpySE526I ACGT 2 cut(s) 1137, 1146
Hsp92II CATG 4 cut(s) 245, 869, 1114, 1243
HspAI GCGC 3 cut(s) 320, 610, 1155
Kpn2I TCCGGA 1 cut(s) 799
Ksp22I TGATCA 2 cut(s) 250, 1335
Kzo9I GATC 8 cut(s) 250, 544, 716, 727, 802, 1121, 1304, 1335
LguI GCTCTTC 2 cut(s) 190, 824
LmnI GCTCC 1 cut(s) 324
Lsp1109I GCAGC 3 cut(s) 349, 917, 970
LweI GCATC 2 cut(s) 506, 1166
MaeI CTAG 2 cut(s) 1019, 1283
MaeII ACGT 2 cut(s) 1137, 1146
MaeIII GTNAC 7 cut(s) 76, 244, 745, 1060, 1093, 1147, 1171
MalI GATC 8 cut(s) 252, 546, 718, 729, 804, 1123, 1306, 1337
MboI GATC 8 cut(s) 250, 544, 716, 727, 802, 1121, 1304, 1335
MboII GAAGA 6 cut(s) 201, 207, 811, 1040, 1244, 1247
MflI RGATCY 1 cut(s) 1121
MhlI GDGCHC 1 cut(s) 591
MluCI AATT 3 cut(s) 386, 484, 757
MluI ACGCGT 1 cut(s) 1225
MlyI GAGTC 2 cut(s) 103, 1057
MroI TCCGGA 1 cut(s) 799
MroXI GAANNNNTTC 1 cut(s) 1139
MseI TTAA 3 cut(s) 117, 582, 839
MslI CAYNNNNRTG 1 cut(s) 924
MspI CCGG 3 cut(s) 465, 800, 1044
Mva1269I GAATGC 1 cut(s) 294
MvnI CGCG 2 cut(s) 322, 1227
MwoI GCNNNNNNNGC 2 cut(s) 437, 623
NdeII GATC 8 cut(s) 250, 544, 716, 727, 802, 1121, 1304, 1335
NlaIII CATG 4 cut(s) 245, 869, 1114, 1243
NlaIV GGNNCC 3 cut(s) 312, 479, 1123
NmuCI GTSAC 5 cut(s) 244, 1060, 1093, 1147, 1171
NsbI TGCGCA 1 cut(s) 611
NspI RCATGY 1 cut(s) 1243
NspV TTCGAA 1 cut(s) 991
PagI TCATGA 1 cut(s) 1110
PciI ACATGT 1 cut(s) 1239
PciSI GCTCTTC 2 cut(s) 190, 824
PcsI WCGNNNNNNNCGW 1 cut(s) 1143
PctI GAATGC 1 cut(s) 294
PdmI GAANNNNTTC 1 cut(s) 1139
PfeI GAWTC 3 cut(s) 181, 710, 1275
PflMI CCANNNNNTGG 1 cut(s) 976
PkrI GCNGC 3 cut(s) 364, 932, 985
PleI GAGTC 2 cut(s) 103, 1057
PpsI GAGTC 2 cut(s) 103, 1057
Ppu21I YACGTR 1 cut(s) 1147
PscI ACATGT 1 cut(s) 1239
Psp1406I AACGTT 1 cut(s) 1137
PspN4I GGNNCC 3 cut(s) 312, 479, 1123
PspPI GGNCC 3 cut(s) 311, 477, 887
PsrI GAACNNNNNNTAC 2 cut(s) 1127, 1159
PstNI CAGNNNCTG 3 cut(s) 49, 307, 713
PsuI RGATCY 1 cut(s) 1121
RsaI GTAC 1 cut(s) 1244
RsaNI GTAC 1 cut(s) 1243
RseI CAYNNNNRTG 1 cut(s) 924
SapI GCTCTTC 2 cut(s) 190, 824
SaqAI TTAA 3 cut(s) 117, 582, 839
SatI GCNGC 3 cut(s) 363, 931, 984
Sau3AI GATC 8 cut(s) 250, 544, 716, 727, 802, 1121, 1304, 1335
Sau96I GGNCC 3 cut(s) 311, 477, 887
SchI GAGTC 2 cut(s) 103, 1057
SduI GDGCHC 1 cut(s) 591
SfaNI GCATC 2 cut(s) 506, 1166
SfcI CTRYAG 1 cut(s) 633
SfuI TTCGAA 1 cut(s) 991
SinI GGWCC 3 cut(s) 311, 477, 887
SmiMI CAYNNNNRTG 1 cut(s) 924
SmlI CTYRAG 3 cut(s) 337, 590, 790
SmoI CTYRAG 3 cut(s) 337, 590, 790
Sse9I AATT 3 cut(s) 386, 484, 757
SsiI CCGC 1 cut(s) 322
SspI AATATT 1 cut(s) 908
SspMI CTAG 2 cut(s) 1019, 1283
StyI CCWWGG 1 cut(s) 71
TaaI ACNGT 8 cut(s) 82, 303, 686, 704, 751, 781, 829, 874
TaiI ACGT 2 cut(s) 1140, 1149
TaqI TCGA 4 cut(s) 384, 730, 822, 991
TasI AATT 3 cut(s) 386, 484, 757
TatI WGTACW 1 cut(s) 1242
TfiI GAWTC 3 cut(s) 181, 710, 1275
Tru1I TTAA 3 cut(s) 117, 582, 839
Tru9I TTAA 3 cut(s) 117, 582, 839
TscAI CASTG 6 cut(s) 253, 619, 628, 709, 730, 1176
TseFI GTSAC 5 cut(s) 244, 1060, 1093, 1147, 1171
TseI GCWGC 3 cut(s) 362, 930, 983
Tsp45I GTSAC 5 cut(s) 244, 1060, 1093, 1147, 1171
TspDTI ATGAA 1 cut(s) 230
TspGWI ACGGA 1 cut(s) 320
TspRI CASTG 6 cut(s) 253, 619, 628, 709, 730, 1176
Van91I CCANNNNNTGG 1 cut(s) 976
VpaK11BI GGWCC 3 cut(s) 311, 477, 887
XceI RCATGY 1 cut(s) 1243
XmnI GAANNNNTTC 1 cut(s) 1139
XspI CTAG 2 cut(s) 1019, 1283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.