Rroxscaffold_5G00371350

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0016314
Physical Location & Seq
Reverse (-)
52933330 .. 52936530
3201 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00371350.1

Sequence Viewer

Length: 1308 bp
ATGGATTGTTGGTCTCCTTCTCTTGCTGTTGACGACGAGTTTCAGAAGCTGGCGTTGCGAGTGAACCCTCCAAGGGTTACAGTTGATAATGGCTCAAGCAGGAAAGCCACTTTGATTAAGGTGATTGAATTTCTTGAATTGTCTCTGCAAGCATTTCAAAAAGGGTCGCCCTCTGAACATTTGGTTCTTATGTTTCATGTCACTGATCAACAGGGGAATAAGCTCTGTGATGATGGAATAGCTGAGCGCATTCAACAGTCTCTAGGACCAAGAGCTCGGAGCTTCCGTGCCTTGAGAAGTTCAGTGGGCGTCCAAGCTGCCACTGAACACACTACTATTGAATTGACTGGAAGGGACAGGGCAGGCTTGCTTTCAGAGGTTTTTGCTGTTCTTGCTGAGCTCAGATGTAATGTGGTTGCCGGAGAAGTATGGACCCATAATTCAAGAATGGCATCAGTTGTTTACATCACGGATGGGCAAACTGGGAGGCCTATTGATGATTCTGAGCGTCTTTCTGAAATTAAAGAGTTTCTCCTTAATGTGCTCAAGGCAGACAAAGATATGCGCTCTGCCAACACTGCCGTTTCTGTAGGTTCTACACACAAGGAGAGGAGGCTTCACCAGATGATGTATGCAGATCGTGATTATGATCTACACAGTGCAGATTCTGGATCAGCCAGTGATAGAAGCAAACCCTTTGTAACTGTCGAAAATTGTGCAGATAAAGGATACACTGTTGTGAACTTGAGGTGTCCAGATCGTCCTAAGTTGCTCTTTGATACAGTATGCACCTTAACAGATATGCAATATGTGGTATATCATGCAACTGTGATTGCTGAAGGACCAGAGGCTTACCAGGAATATTATATAAGACATACTGATGGCTGCCCTATAAGTTCTGAAGGAGAGAGGCAGAGGGTAATCCACTGCTTGGAGGCTGCTATTCGAAGGCGAACTTCTGAGGGTATAAGACTAGAACTCTGTGGTGATGACAGAGCCGGTCTTCTTTCTGACGTGACTCGCATATTCAGAGAAAACAGCCTTTCAGTCACCCGAGCTGAGGTTATGACCAGGGGTTCCCAAGCTGTGAACGTCTTCTACGTGACTGGTGCATGTGGAAATCCAGTGACGAGTGAGACAATTGAGGCAGTTCGGCAAGAGATTGGCTTGACAATACTGCGCGTAGAAGAAGACATGTACTCAAAATCTCCACCACAGGAGAGTGGGAGATTCTCTTTGGGAAATCTATTCCGGACCAGATCAGAGAAATTCCTTTACAACTTGGGTTTGATAAAGTCGTGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

435

Amino Acids

48.51

Weight (kDa)

6.0

Isoelectric Point (pI)

45.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 111 - 158 7.4e-07 ACT domain
ACT PF01842 325 - 386 1.5e-06 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016314)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23320 FvH4_4g23320
malus_domestica MD13G1123000.v1.1 MD16G1123700.v1.1
prunus_persica Prupe.1G221900_v2.0.a1
pyrus_communis pycom13g10740 pycom16g10570
rosa_chinensis RchiOBHm_Chr4g0429671
rosa_laevigata RLG00000007053
rosa_multiflora Rmu_sc0010349.1_g000004
rosa_roxburghii Rroxscaffold_5G00371350
rosa_rugosa Rorug04G0237600
rosa_samantha Rh4AG293700 Rh4DG296600
rosa_wichuraiana Rw4G025420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 931
AccII CGCG 1 cut(s) 1182
AccIII TCCGGA 1 cut(s) 1251
AclWI GGATC 1 cut(s) 679
AcsI RAATTY 2 cut(s) 128, 1268
AcuI CTGAAG 2 cut(s) 858, 921
AcyI GRCGYC 1 cut(s) 309
AfaI GTAC 1 cut(s) 1199
AfiI CCNNNNNNNGG 3 cut(s) 73, 931, 1060
AflIII ACRYGT 1 cut(s) 1194
AgsI TTSAA 6 cut(s) 128, 137, 158, 254, 341, 444
AjiI CACGTC 1 cut(s) 1015
AjnI CCWGG 2 cut(s) 855, 1070
AleI CACNNNNGTG 1 cut(s) 737
AluBI AGCT 9 cut(s) 49, 223, 242, 275, 282, 317, 400, 1058, 1085
AluI AGCT 9 cut(s) 49, 223, 242, 275, 282, 317, 400, 1058, 1085
Alw21I GWGCWC 3 cut(s) 277, 402, 546
Alw26I GTCTC 4 cut(s) 18, 147, 264, 1130
AlwI GGATC 1 cut(s) 679
AlwNI CAGNNNCTG 2 cut(s) 49, 668
Ama87I CYCGRG 1 cut(s) 1053
Aor13HI TCCGGA 1 cut(s) 1251
AoxI GGCC 1 cut(s) 488
ApeKI GCWGC 3 cut(s) 317, 885, 938
ApoI RAATTY 2 cut(s) 128, 1268
Asp700I GAANNNNTTC 1 cut(s) 1094
AspLEI GCGC 3 cut(s) 249, 567, 1182
AspS9I GGNCC 4 cut(s) 266, 432, 842, 1254
AsuHPI GGTGA 4 cut(s) 133, 611, 998, 1042
AsuII TTCGAA 1 cut(s) 946
AvaI CYCGRG 1 cut(s) 1053
AvaII GGWCC 4 cut(s) 266, 432, 842, 1254
BanII GRGCYC 2 cut(s) 277, 402
BbsI GAAGAC 3 cut(s) 995, 1087, 1197
Bbv12I GWGCWC 3 cut(s) 277, 402, 546
BbvCI CCTCAGC 1 cut(s) 1059
BbvI GCAGC 3 cut(s) 304, 872, 925
BccI CCATC 3 cut(s) 227, 467, 875
BceAI ACGGC 1 cut(s) 566
BcgI CGANNNNNNTGC 2 cut(s) 698, 732
BciT130I CCWGG 2 cut(s) 857, 1072
BciVI GTATCC 1 cut(s) 722
BclI TGATCA 1 cut(s) 205
BcoDI GTCTC 4 cut(s) 18, 147, 264, 1130
BfaI CTAG 2 cut(s) 263, 974
BfmI CTRYAG 1 cut(s) 588
BfuI GTATCC 1 cut(s) 722
BisI GCNGC 3 cut(s) 318, 886, 939
BlpI GCTNAGC 2 cut(s) 243, 396
BlsI GCNGC 3 cut(s) 319, 887, 940
Bme1390I CCNGG 2 cut(s) 857, 1072
Bme18I GGWCC 4 cut(s) 266, 432, 842, 1254
BmeT110I CYCGRG 1 cut(s) 1053
BmgBI CACGTC 1 cut(s) 1015
BmgT120I GGNCC 4 cut(s) 266, 432, 842, 1254
BmiI GGNNCC 2 cut(s) 434, 1078
BmrFI CCNGG 2 cut(s) 857, 1072
BmrI ACTGGG 1 cut(s) 492
BmsI GCATC 1 cut(s) 461
BmuI ACTGGG 1 cut(s) 492
BpiI GAAGAC 3 cut(s) 995, 1087, 1197
Bpu10I CCTNAGC 1 cut(s) 1059
Bpu1102I GCTNAGC 2 cut(s) 243, 396
Bpu14I TTCGAA 1 cut(s) 946
BpuEI CTTGAG 4 cut(s) 79, 313, 530, 766
BsaAI YACGTR 1 cut(s) 1102
BsaBI GATNNNNATC 1 cut(s) 648
BsaHI GRCGYC 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 18
BsaJI CCNNGG 2 cut(s) 71, 1071
BsaWI WCCGGW 1 cut(s) 1251
BsaXI ACNNNNNCTCC 2 cut(s) 271, 301
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 931, 1060
Bse118I RCCGGY 1 cut(s) 998
Bse1I ACTGG 5 cut(s) 352, 487, 678, 1111, 1124
Bse8I GATNNNNATC 1 cut(s) 648
BseAI TCCGGA 1 cut(s) 1251
BseBI CCWGG 2 cut(s) 857, 1072
BseDI CCNNGG 2 cut(s) 71, 1071
BseGI GGATG 1 cut(s) 478
BseJI GATNNNNATC 1 cut(s) 648
BseLI CCNNNNNNNGG 3 cut(s) 73, 931, 1060
BseMII CTCAG 6 cut(s) 234, 387, 415, 495, 951, 1050
BseNI ACTGG 5 cut(s) 352, 487, 678, 1111, 1124
BseRI GAGGAG 1 cut(s) 625
BseXI GCAGC 3 cut(s) 304, 872, 925
BsgI GTGCAG 2 cut(s) 681, 738
Bsh1236I CGCG 1 cut(s) 1182
BshFI GGCC 1 cut(s) 490
BsiHKAI GWGCWC 3 cut(s) 277, 402, 546
BsiHKCI CYCGRG 1 cut(s) 1053
BsiSI CCGG 3 cut(s) 420, 999, 1252
BslFI GGGAC 1 cut(s) 368
BslI CCNNNNNNNGG 3 cut(s) 73, 931, 1060
BsmAI GTCTC 4 cut(s) 18, 147, 264, 1130
BsmFI GGGAC 1 cut(s) 368
BsmI GAATGC 1 cut(s) 249
BsnI GGCC 1 cut(s) 490
Bso31I GGTCTC 1 cut(s) 18
BsoBI CYCGRG 1 cut(s) 1053
Bsp119I TTCGAA 1 cut(s) 946
Bsp1286I GDGCHC 3 cut(s) 277, 402, 546
Bsp13I TCCGGA 1 cut(s) 1251
Bsp143I GATC 6 cut(s) 205, 637, 649, 671, 757, 1259
Bsp1720I GCTNAGC 2 cut(s) 243, 396
BspANI GGCC 1 cut(s) 490
BspCNI CTCAG 6 cut(s) 235, 388, 414, 496, 952, 1051
BspEI TCCGGA 1 cut(s) 1251
BspFNI CGCG 1 cut(s) 1182
BspLI GGNNCC 2 cut(s) 434, 1078
BspPI GGATC 1 cut(s) 679
BspT104I TTCGAA 1 cut(s) 946
BspTNI GGTCTC 1 cut(s) 18
BsrFI RCCGGY 1 cut(s) 998
BsrI ACTGG 5 cut(s) 352, 487, 678, 1111, 1124
BssAI RCCGGY 1 cut(s) 998
BssECI CCNNGG 2 cut(s) 71, 1071
BssMI GATC 6 cut(s) 205, 637, 649, 671, 757, 1259
BssNI GRCGYC 1 cut(s) 309
BssT1I CCWWGG 1 cut(s) 71
Bst2UI CCWGG 2 cut(s) 857, 1072
Bst4CI ACNGT 7 cut(s) 82, 258, 659, 706, 736, 784, 829
BstACI GRCGYC 1 cut(s) 309
BstBAI YACGTR 1 cut(s) 1102
BstBI TTCGAA 1 cut(s) 946
BstC8I GCNNGC 4 cut(s) 51, 150, 364, 368
BstDEI CTNAG 7 cut(s) 243, 396, 401, 504, 765, 960, 1059
BstF5I GGATG 1 cut(s) 478
BstFNI CGCG 1 cut(s) 1182
BstHHI GCGC 3 cut(s) 249, 567, 1182
BstKTI GATC 6 cut(s) 208, 640, 652, 674, 760, 1262
BstMAI GTCTC 4 cut(s) 18, 147, 264, 1130
BstMBI GATC 6 cut(s) 205, 637, 649, 671, 757, 1259
BstMWI GCNNNNNNNGC 3 cut(s) 55, 392, 578
BstNI CCWGG 2 cut(s) 857, 1072
BstNSI RCATGY 2 cut(s) 1116, 1198
BstSCI CCNGG 2 cut(s) 855, 1070
BstSFI CTRYAG 1 cut(s) 588
BstUI CGCG 1 cut(s) 1182
BstV1I GCAGC 3 cut(s) 304, 872, 925
BstV2I GAAGAC 3 cut(s) 995, 1087, 1197
BsuI GTATCC 1 cut(s) 722
BsuRI GGCC 1 cut(s) 490
BtrI CACGTC 1 cut(s) 1015
BtsCI GGATG 1 cut(s) 478
BtsI GCAGTG 2 cut(s) 576, 925
BtsIMutI CAGTG 9 cut(s) 201, 309, 321, 576, 664, 685, 732, 925, 1131
Cac8I GCNNGC 4 cut(s) 51, 150, 364, 368
CaiI CAGNNNCTG 2 cut(s) 49, 668
CfoI GCGC 3 cut(s) 249, 567, 1182
Cfr10I RCCGGY 1 cut(s) 998
Cfr13I GGNCC 4 cut(s) 266, 432, 842, 1254
CseI GACGC 2 cut(s) 298, 497
Csp6I GTAC 1 cut(s) 1198
CviAII CATG 4 cut(s) 197, 821, 1113, 1195
CviQI GTAC 1 cut(s) 1198
DdeI CTNAG 7 cut(s) 243, 396, 401, 504, 765, 960, 1059
DpnI GATC 6 cut(s) 207, 639, 651, 673, 759, 1261
DpnII GATC 6 cut(s) 205, 637, 649, 671, 757, 1259
Ecl136II GAGCTC 2 cut(s) 275, 400
Eco130I CCWWGG 1 cut(s) 71
Eco147I AGGCCT 1 cut(s) 490
Eco24I GRGCYC 2 cut(s) 277, 402
Eco31I GGTCTC 1 cut(s) 18
Eco47I GGWCC 4 cut(s) 266, 432, 842, 1254
Eco53kI GAGCTC 2 cut(s) 275, 400
Eco57I CTGAAG 2 cut(s) 858, 921
Eco88I CYCGRG 1 cut(s) 1053
EcoICRI GAGCTC 2 cut(s) 275, 400
EcoRII CCWGG 2 cut(s) 855, 1070
EcoT14I CCWWGG 1 cut(s) 71
EcoT38I GRGCYC 2 cut(s) 277, 402
ErhI CCWWGG 1 cut(s) 71
FaeI CATG 4 cut(s) 200, 824, 1116, 1198
FalI AAGNNNNNCTT 4 cut(s) 758, 790, 940, 972
FaqI GGGAC 1 cut(s) 368
FatI CATG 4 cut(s) 196, 820, 1112, 1194
FbaI TGATCA 1 cut(s) 205
Fnu4HI GCNGC 3 cut(s) 318, 886, 939
FokI GGATG 1 cut(s) 485
FriOI GRGCYC 2 cut(s) 277, 402
Fsp4HI GCNGC 3 cut(s) 318, 886, 939
FspBI CTAG 2 cut(s) 263, 974
GlaI GCGC 3 cut(s) 248, 566, 1181
GluI GCNGC 3 cut(s) 318, 886, 939
HaeIII GGCC 1 cut(s) 490
HapII CCGG 3 cut(s) 420, 999, 1252
HgaI GACGC 2 cut(s) 298, 497
HhaI GCGC 3 cut(s) 249, 567, 1182
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 4 cut(s) 200, 824, 1116, 1198
Hin6I GCGC 3 cut(s) 247, 565, 1180
HinP1I GCGC 3 cut(s) 247, 565, 1180
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HinfI GANTC 4 cut(s) 500, 665, 1018, 1230
HpaII CCGG 3 cut(s) 420, 999, 1252
HphI GGTGA 4 cut(s) 133, 611, 998, 1042
Hpy166II GTNNAC 5 cut(s) 31, 64, 463, 742, 1090
Hpy188III TCNNGA 7 cut(s) 134, 444, 641, 669, 755, 1252, 1305
Hpy8I GTNNAC 5 cut(s) 31, 64, 463, 742, 1090
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 5 cut(s) 27, 345, 833, 896, 942
HpyCH4III ACNGT 7 cut(s) 82, 258, 659, 706, 736, 784, 829
HpyCH4IV ACGT 3 cut(s) 1014, 1092, 1101
HpyCH4V TGCA 8 cut(s) 148, 635, 662, 719, 789, 805, 824, 1112
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 392, 578
HpyF3I CTNAG 7 cut(s) 243, 396, 401, 504, 765, 960, 1059
HpySE526I ACGT 3 cut(s) 1014, 1092, 1101
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 4 cut(s) 200, 824, 1116, 1198
HspAI GCGC 3 cut(s) 247, 565, 1180
Kpn2I TCCGGA 1 cut(s) 1251
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 6 cut(s) 205, 637, 649, 671, 757, 1259
LmnI GCTCC 1 cut(s) 279
Lsp1109I GCAGC 3 cut(s) 304, 872, 925
LweI GCATC 1 cut(s) 461
MaeI CTAG 2 cut(s) 263, 974
MaeII ACGT 3 cut(s) 1014, 1092, 1101
MaeIII GTNAC 7 cut(s) 76, 199, 700, 1015, 1048, 1102, 1126
MalI GATC 6 cut(s) 207, 639, 651, 673, 759, 1261
MboI GATC 6 cut(s) 205, 637, 649, 671, 757, 1259
MboII GAAGA 4 cut(s) 995, 1087, 1199, 1202
MfeI CAATTG 1 cut(s) 1140
MhlI GDGCHC 3 cut(s) 277, 402, 546
MluCI AATT 8 cut(s) 128, 137, 341, 439, 519, 712, 1140, 1268
MlyI GAGTC 1 cut(s) 1012
MroI TCCGGA 1 cut(s) 1251
MroXI GAANNNNTTC 1 cut(s) 1094
MseI TTAA 4 cut(s) 117, 522, 537, 794
MslI CAYNNNNRTG 2 cut(s) 737, 879
MspI CCGG 3 cut(s) 420, 999, 1252
MspR9I CCNGG 2 cut(s) 857, 1072
MunI CAATTG 1 cut(s) 1140
Mva1269I GAATGC 1 cut(s) 249
MvaI CCWGG 2 cut(s) 857, 1072
MvnI CGCG 1 cut(s) 1182
MwoI GCNNNNNNNGC 3 cut(s) 55, 392, 578
NdeII GATC 6 cut(s) 205, 637, 649, 671, 757, 1259
NlaIII CATG 4 cut(s) 200, 824, 1116, 1198
NlaIV GGNNCC 2 cut(s) 434, 1078
NmuCI GTSAC 5 cut(s) 199, 1015, 1048, 1102, 1126
NspI RCATGY 2 cut(s) 1116, 1198
NspV TTCGAA 1 cut(s) 946
OliI CACNNNNGTG 1 cut(s) 737
PceI AGGCCT 1 cut(s) 490
PciI ACATGT 1 cut(s) 1194
PcsI WCGNNNNNNNCGW 2 cut(s) 283, 1098
PctI GAATGC 1 cut(s) 249
PdmI GAANNNNTTC 1 cut(s) 1094
PfeI GAWTC 3 cut(s) 500, 665, 1230
PflMI CCANNNNNTGG 1 cut(s) 931
PkrI GCNGC 3 cut(s) 319, 887, 940
PleI GAGTC 1 cut(s) 1012
PpsI GAGTC 1 cut(s) 1012
Ppu21I YACGTR 1 cut(s) 1102
PscI ACATGT 1 cut(s) 1194
Psp124BI GAGCTC 2 cut(s) 277, 402
Psp6I CCWGG 2 cut(s) 855, 1070
PspGI CCWGG 2 cut(s) 855, 1070
PspN4I GGNNCC 2 cut(s) 434, 1078
PspPI GGNCC 4 cut(s) 266, 432, 842, 1254
PsrI GAACNNNNNNTAC 2 cut(s) 1082, 1114
PstNI CAGNNNCTG 2 cut(s) 49, 668
RsaI GTAC 1 cut(s) 1199
RsaNI GTAC 1 cut(s) 1198
RseI CAYNNNNRTG 2 cut(s) 737, 879
SacI GAGCTC 2 cut(s) 277, 402
SaqAI TTAA 4 cut(s) 117, 522, 537, 794
SatI GCNGC 3 cut(s) 318, 886, 939
Sau3AI GATC 6 cut(s) 205, 637, 649, 671, 757, 1259
Sau96I GGNCC 4 cut(s) 266, 432, 842, 1254
SchI GAGTC 1 cut(s) 1012
ScrFI CCNGG 2 cut(s) 857, 1072
SduI GDGCHC 3 cut(s) 277, 402, 546
SfaNI GCATC 1 cut(s) 461
SfcI CTRYAG 1 cut(s) 588
SfuI TTCGAA 1 cut(s) 946
SinI GGWCC 4 cut(s) 266, 432, 842, 1254
SmiMI CAYNNNNRTG 2 cut(s) 737, 879
SmlI CTYRAG 4 cut(s) 94, 292, 545, 745
SmoI CTYRAG 4 cut(s) 94, 292, 545, 745
Sse9I AATT 8 cut(s) 128, 137, 341, 439, 519, 712, 1140, 1268
SseBI AGGCCT 1 cut(s) 490
SspI AATATT 1 cut(s) 863
SspMI CTAG 2 cut(s) 263, 974
SstI GAGCTC 2 cut(s) 277, 402
StuI AGGCCT 1 cut(s) 490
StyD4I CCNGG 2 cut(s) 855, 1070
StyI CCWWGG 1 cut(s) 71
TaaI ACNGT 7 cut(s) 82, 258, 659, 706, 736, 784, 829
TaiI ACGT 3 cut(s) 1017, 1095, 1104
TaqI TCGA 2 cut(s) 708, 946
TasI AATT 8 cut(s) 128, 137, 341, 439, 519, 712, 1140, 1268
TatI WGTACW 1 cut(s) 1197
TfiI GAWTC 3 cut(s) 500, 665, 1230
Tru1I TTAA 4 cut(s) 117, 522, 537, 794
Tru9I TTAA 4 cut(s) 117, 522, 537, 794
TscAI CASTG 9 cut(s) 208, 309, 328, 583, 664, 685, 739, 932, 1131
TseFI GTSAC 5 cut(s) 199, 1015, 1048, 1102, 1126
TseI GCWGC 3 cut(s) 317, 885, 938
Tsp45I GTSAC 5 cut(s) 199, 1015, 1048, 1102, 1126
TspDTI ATGAA 1 cut(s) 185
TspGWI ACGGA 2 cut(s) 275, 485
TspRI CASTG 9 cut(s) 208, 309, 328, 583, 664, 685, 739, 932, 1131
Van91I CCANNNNNTGG 1 cut(s) 931
VpaK11BI GGWCC 4 cut(s) 266, 432, 842, 1254
XapI RAATTY 2 cut(s) 128, 1268
XceI RCATGY 2 cut(s) 1116, 1198
XmnI GAANNNNTTC 1 cut(s) 1094
XspI CTAG 2 cut(s) 263, 974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.