RLG00000007053

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0016314
Physical Location & Seq
Forward (+)
13113764 .. 13116276
2513 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007053

Sequence Viewer

Length: 1341 bp
ATGGATTGTTGGTCTCCTTCTCTTGCTGTTGACGACGAGTTTCAGAAGCTGGCGTTGCGAGTGAACCCTCCAAGGGTTACAGTTGATAATGGCTCAAGCAGGAAAGCCACTTTGATTAAGGTGATTGAATTTCTTGAATTGTCTTTGCAAGCATTTCAAAAAGGGTCCCCCTCTGAACATTTGGTTCTTTGTCTCTTTCTTTTTTTTGGGGGAATTTTTACAGTGTTTCATGTCACTGATCAACAGGGGAATAAGCTCTGTGACGATGGAATAGCTGAGCGCATTCAACAGTCTCTAGGACCAAGAGCTCGGAGCTTCCGTGCCTTGAGAAGTTCAGTGGGCGTCCAAGCTGCCACAGAACACACTACTATTGAATTGACGGGAAGGGACAGGGCAGGCTTGCTTTCAGAGGTTTTTGCTGTTCTTGCTGAGCTCAGATGTAATGTGGTTGCCGGAGAAGTATGGACCCATAATTCAAGAATGGCATCAGTTGTTTACATCACTGATGGGCAAACTGGGAGGCCTATTGATGATTCTGAGCGTCTTTCTGAAATTAAAGAGTTTCTCCTTAATGTGCTCAAGGCAGACAAAGATATGCGCTCTGCCAACACTGCCGTTTCTGTAGGTTCTACACACAAGGAGAGGAGGCTTCACCAGATGATGTATGCAGATCGTGATTATGATCTACACAGTGCAGATTCTGGATCAGCCAGTGATAGAAGCAAACCCTTTGTAACTGTCGAAAATTGTGCAGATAAAGGATATACTGTTGTGAACTTGAGGTGTCCAGATCGTCCTAAGTTGCTCTTTGATACAGTATGCACCTTAACAGATATGCAATATGTGGTATATCATGCAACTGTGATTGCTGAAGGACCAGAGGCTTATCAGGAATATTATATAAGACATACAGATGGCTGCCCTATAAGTTCTGAAGGAGAGAGGCAGAGGGTAATCCACTGCTTGGAGGCTGCTATTCGAAGGCGAACTTCTGAGGGTATAAGACTAGAACTCTGTGGTGATGACAGAGCCGGTCTTCTTTCTGACGTGACTCGCATATTCAGAGAAAACAGCCTTTCAGTCACCCGAGCTGAGGTTATGACCAGGGGTTCCCAAGCTGTGAACGTCTTCTACGTGACTGGTGCATCTGGAAATCCAGTGACGAGTGAGACAATTGAGGCAGTTCGGCAAGAGATTGGCTTGACAATACTGCGCGTAGAAGAAGACGTGTACTCAAAATCTCCACCACAGGAGAGTGGGAGATTCTCTTTGGGAAATCTATTCCGGACGAGATCAGAGAAATTCCTTTACAACTTGGGTTTGATAAAGTCGTGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

447

Amino Acids

49.68

Weight (kDa)

6.0

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 122 - 169 7.6e-07 ACT domain
ACT PF01842 336 - 395 1.9e-07 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016314)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23320 FvH4_4g23320
malus_domestica MD13G1123000.v1.1 MD16G1123700.v1.1
prunus_persica Prupe.1G221900_v2.0.a1
pyrus_communis pycom13g10740 pycom16g10570
rosa_chinensis RchiOBHm_Chr4g0429671
rosa_laevigata RLG00000007053
rosa_multiflora Rmu_sc0010349.1_g000004
rosa_roxburghii Rroxscaffold_5G00371350
rosa_rugosa Rorug04G0237600
rosa_samantha Rh4AG293700 Rh4DG296600
rosa_wichuraiana Rw4G025420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 964
AccII CGCG 1 cut(s) 1215
AccIII TCCGGA 1 cut(s) 1284
AclWI GGATC 1 cut(s) 712
AcsI RAATTY 3 cut(s) 128, 213, 1301
AcuI CTGAAG 2 cut(s) 891, 954
AcyI GRCGYC 1 cut(s) 342
AfaI GTAC 1 cut(s) 1232
AfiI CCNNNNNNNGG 3 cut(s) 73, 964, 1093
AflIII ACRYGT 1 cut(s) 1227
AgsI TTSAA 6 cut(s) 128, 137, 158, 287, 374, 477
AjiI CACGTC 2 cut(s) 1048, 1228
AjnI CCWGG 1 cut(s) 1103
AluBI AGCT 9 cut(s) 49, 256, 275, 308, 315, 350, 433, 1091, 1118
AluI AGCT 9 cut(s) 49, 256, 275, 308, 315, 350, 433, 1091, 1118
Alw21I GWGCWC 3 cut(s) 310, 435, 579
Alw26I GTCTC 4 cut(s) 18, 197, 297, 1163
AlwI GGATC 1 cut(s) 712
AlwNI CAGNNNCTG 2 cut(s) 49, 701
Ama87I CYCGRG 1 cut(s) 1086
Aor13HI TCCGGA 1 cut(s) 1284
AoxI GGCC 1 cut(s) 521
ApeKI GCWGC 3 cut(s) 350, 918, 971
ApoI RAATTY 3 cut(s) 128, 213, 1301
Asp700I GAANNNNTTC 1 cut(s) 1127
AspLEI GCGC 3 cut(s) 282, 600, 1215
AspS9I GGNCC 4 cut(s) 165, 299, 465, 875
AsuHPI GGTGA 4 cut(s) 133, 644, 1031, 1075
AsuII TTCGAA 1 cut(s) 979
AvaI CYCGRG 1 cut(s) 1086
AvaII GGWCC 4 cut(s) 165, 299, 465, 875
BanII GRGCYC 2 cut(s) 310, 435
BbsI GAAGAC 3 cut(s) 1028, 1120, 1230
Bbv12I GWGCWC 3 cut(s) 310, 435, 579
BbvCI CCTCAGC 1 cut(s) 1092
BbvI GCAGC 3 cut(s) 337, 905, 958
BccI CCATC 3 cut(s) 260, 500, 908
BceAI ACGGC 1 cut(s) 599
BcgI CGANNNNNNTGC 2 cut(s) 731, 765
BciT130I CCWGG 1 cut(s) 1105
BclI TGATCA 1 cut(s) 238
BcoDI GTCTC 4 cut(s) 18, 197, 297, 1163
BfaI CTAG 2 cut(s) 296, 1007
BfmI CTRYAG 1 cut(s) 621
BisI GCNGC 3 cut(s) 351, 919, 972
BlpI GCTNAGC 2 cut(s) 276, 429
BlsI GCNGC 3 cut(s) 352, 920, 973
Bme1390I CCNGG 1 cut(s) 1105
Bme18I GGWCC 4 cut(s) 165, 299, 465, 875
BmeT110I CYCGRG 1 cut(s) 1086
BmgBI CACGTC 2 cut(s) 1048, 1228
BmgT120I GGNCC 4 cut(s) 165, 299, 465, 875
BmiI GGNNCC 4 cut(s) 166, 167, 467, 1111
BmrFI CCNGG 1 cut(s) 1105
BmrI ACTGGG 1 cut(s) 525
BmsI GCATC 2 cut(s) 494, 1154
BmuI ACTGGG 1 cut(s) 525
BpiI GAAGAC 3 cut(s) 1028, 1120, 1230
Bpu10I CCTNAGC 1 cut(s) 1092
Bpu1102I GCTNAGC 2 cut(s) 276, 429
Bpu14I TTCGAA 1 cut(s) 979
BpuEI CTTGAG 4 cut(s) 79, 346, 563, 799
BsaAI YACGTR 1 cut(s) 1135
BsaBI GATNNNNATC 1 cut(s) 681
BsaHI GRCGYC 1 cut(s) 342
BsaI GGTCTC 1 cut(s) 18
BsaJI CCNNGG 2 cut(s) 71, 1104
BsaWI WCCGGW 1 cut(s) 1284
BsaXI ACNNNNNCTCC 2 cut(s) 304, 334
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 964, 1093
Bse118I RCCGGY 1 cut(s) 1031
Bse1I ACTGG 4 cut(s) 520, 711, 1144, 1157
Bse8I GATNNNNATC 1 cut(s) 681
BseAI TCCGGA 1 cut(s) 1284
BseBI CCWGG 1 cut(s) 1105
BseDI CCNNGG 2 cut(s) 71, 1104
BseJI GATNNNNATC 1 cut(s) 681
BseLI CCNNNNNNNGG 3 cut(s) 73, 964, 1093
BseMII CTCAG 6 cut(s) 267, 420, 448, 528, 984, 1083
BseNI ACTGG 4 cut(s) 520, 711, 1144, 1157
BseRI GAGGAG 1 cut(s) 658
BseXI GCAGC 3 cut(s) 337, 905, 958
BsgI GTGCAG 2 cut(s) 714, 771
Bsh1236I CGCG 1 cut(s) 1215
BshFI GGCC 1 cut(s) 523
BsiHKAI GWGCWC 3 cut(s) 310, 435, 579
BsiHKCI CYCGRG 1 cut(s) 1086
BsiSI CCGG 3 cut(s) 453, 1032, 1285
BslFI GGGAC 2 cut(s) 151, 401
BslI CCNNNNNNNGG 3 cut(s) 73, 964, 1093
BsmAI GTCTC 4 cut(s) 18, 197, 297, 1163
BsmFI GGGAC 2 cut(s) 151, 401
BsmI GAATGC 1 cut(s) 282
BsnI GGCC 1 cut(s) 523
Bso31I GGTCTC 1 cut(s) 18
BsoBI CYCGRG 1 cut(s) 1086
Bsp119I TTCGAA 1 cut(s) 979
Bsp1286I GDGCHC 3 cut(s) 310, 435, 579
Bsp13I TCCGGA 1 cut(s) 1284
Bsp143I GATC 6 cut(s) 238, 670, 682, 704, 790, 1292
Bsp1720I GCTNAGC 2 cut(s) 276, 429
BspANI GGCC 1 cut(s) 523
BspCNI CTCAG 6 cut(s) 268, 421, 447, 529, 985, 1084
BspEI TCCGGA 1 cut(s) 1284
BspFNI CGCG 1 cut(s) 1215
BspLI GGNNCC 4 cut(s) 166, 167, 467, 1111
BspPI GGATC 1 cut(s) 712
BspT104I TTCGAA 1 cut(s) 979
BspTNI GGTCTC 1 cut(s) 18
BsrFI RCCGGY 1 cut(s) 1031
BsrI ACTGG 4 cut(s) 520, 711, 1144, 1157
BssAI RCCGGY 1 cut(s) 1031
BssECI CCNNGG 2 cut(s) 71, 1104
BssMI GATC 6 cut(s) 238, 670, 682, 704, 790, 1292
BssNI GRCGYC 1 cut(s) 342
BssT1I CCWWGG 1 cut(s) 71
Bst2UI CCWGG 1 cut(s) 1105
Bst4CI ACNGT 8 cut(s) 82, 223, 291, 692, 739, 769, 817, 862
BstACI GRCGYC 1 cut(s) 342
BstBAI YACGTR 1 cut(s) 1135
BstBI TTCGAA 1 cut(s) 979
BstC8I GCNNGC 4 cut(s) 51, 150, 397, 401
BstDEI CTNAG 7 cut(s) 276, 429, 434, 537, 798, 993, 1092
BstFNI CGCG 1 cut(s) 1215
BstHHI GCGC 3 cut(s) 282, 600, 1215
BstKTI GATC 6 cut(s) 241, 673, 685, 707, 793, 1295
BstMAI GTCTC 4 cut(s) 18, 197, 297, 1163
BstMBI GATC 6 cut(s) 238, 670, 682, 704, 790, 1292
BstMWI GCNNNNNNNGC 3 cut(s) 55, 425, 611
BstNI CCWGG 1 cut(s) 1105
BstSCI CCNGG 1 cut(s) 1103
BstSFI CTRYAG 1 cut(s) 621
BstUI CGCG 1 cut(s) 1215
BstV1I GCAGC 3 cut(s) 337, 905, 958
BstV2I GAAGAC 3 cut(s) 1028, 1120, 1230
BsuRI GGCC 1 cut(s) 523
BtrI CACGTC 2 cut(s) 1048, 1228
BtsI GCAGTG 2 cut(s) 609, 958
BtsIMutI CAGTG 9 cut(s) 228, 234, 342, 501, 609, 697, 718, 958, 1164
Cac8I GCNNGC 4 cut(s) 51, 150, 397, 401
CaiI CAGNNNCTG 2 cut(s) 49, 701
CfoI GCGC 3 cut(s) 282, 600, 1215
Cfr10I RCCGGY 1 cut(s) 1031
Cfr13I GGNCC 4 cut(s) 165, 299, 465, 875
CseI GACGC 2 cut(s) 331, 530
Csp6I GTAC 1 cut(s) 1231
CviAII CATG 2 cut(s) 230, 854
CviQI GTAC 1 cut(s) 1231
DdeI CTNAG 7 cut(s) 276, 429, 434, 537, 798, 993, 1092
DpnI GATC 6 cut(s) 240, 672, 684, 706, 792, 1294
DpnII GATC 6 cut(s) 238, 670, 682, 704, 790, 1292
Ecl136II GAGCTC 2 cut(s) 308, 433
Eco130I CCWWGG 1 cut(s) 71
Eco147I AGGCCT 1 cut(s) 523
Eco24I GRGCYC 2 cut(s) 310, 435
Eco31I GGTCTC 1 cut(s) 18
Eco47I GGWCC 4 cut(s) 165, 299, 465, 875
Eco53kI GAGCTC 2 cut(s) 308, 433
Eco57I CTGAAG 2 cut(s) 891, 954
Eco88I CYCGRG 1 cut(s) 1086
EcoICRI GAGCTC 2 cut(s) 308, 433
EcoO109I RGGNCCY 1 cut(s) 165
EcoRII CCWGG 1 cut(s) 1103
EcoT14I CCWWGG 1 cut(s) 71
EcoT38I GRGCYC 2 cut(s) 310, 435
ErhI CCWWGG 1 cut(s) 71
FaeI CATG 2 cut(s) 233, 857
FalI AAGNNNNNCTT 4 cut(s) 791, 823, 973, 1005
FaqI GGGAC 2 cut(s) 151, 401
FatI CATG 2 cut(s) 229, 853
FbaI TGATCA 1 cut(s) 238
Fnu4HI GCNGC 3 cut(s) 351, 919, 972
FriOI GRGCYC 2 cut(s) 310, 435
Fsp4HI GCNGC 3 cut(s) 351, 919, 972
FspBI CTAG 2 cut(s) 296, 1007
GlaI GCGC 3 cut(s) 281, 599, 1214
GluI GCNGC 3 cut(s) 351, 919, 972
HaeIII GGCC 1 cut(s) 523
HapII CCGG 3 cut(s) 453, 1032, 1285
HgaI GACGC 2 cut(s) 331, 530
HhaI GCGC 3 cut(s) 282, 600, 1215
Hin1I GRCGYC 1 cut(s) 342
Hin1II CATG 2 cut(s) 233, 857
Hin6I GCGC 3 cut(s) 280, 598, 1213
HinP1I GCGC 3 cut(s) 280, 598, 1213
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HinfI GANTC 4 cut(s) 533, 698, 1051, 1263
HpaII CCGG 3 cut(s) 453, 1032, 1285
HphI GGTGA 4 cut(s) 133, 644, 1031, 1075
Hpy166II GTNNAC 6 cut(s) 31, 64, 496, 775, 1123, 1231
Hpy188III TCNNGA 9 cut(s) 134, 477, 674, 702, 788, 890, 1149, 1285, 1338
Hpy8I GTNNAC 6 cut(s) 31, 64, 496, 775, 1123, 1231
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 5 cut(s) 27, 378, 866, 929, 975
HpyCH4III ACNGT 8 cut(s) 82, 223, 291, 692, 739, 769, 817, 862
HpyCH4IV ACGT 4 cut(s) 1047, 1125, 1134, 1227
HpyCH4V TGCA 8 cut(s) 148, 668, 695, 752, 822, 838, 857, 1145
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 425, 611
HpyF3I CTNAG 7 cut(s) 276, 429, 434, 537, 798, 993, 1092
HpySE526I ACGT 4 cut(s) 1047, 1125, 1134, 1227
Hsp92I GRCGYC 1 cut(s) 342
Hsp92II CATG 2 cut(s) 233, 857
HspAI GCGC 3 cut(s) 280, 598, 1213
KflI GGGWCCC 1 cut(s) 165
Kpn2I TCCGGA 1 cut(s) 1284
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 6 cut(s) 238, 670, 682, 704, 790, 1292
LmnI GCTCC 1 cut(s) 312
Lsp1109I GCAGC 3 cut(s) 337, 905, 958
LweI GCATC 2 cut(s) 494, 1154
MaeI CTAG 2 cut(s) 296, 1007
MaeII ACGT 4 cut(s) 1047, 1125, 1134, 1227
MaeIII GTNAC 8 cut(s) 76, 232, 260, 733, 1048, 1081, 1135, 1159
MalI GATC 6 cut(s) 240, 672, 684, 706, 792, 1294
MboI GATC 6 cut(s) 238, 670, 682, 704, 790, 1292
MboII GAAGA 4 cut(s) 1028, 1120, 1232, 1235
MfeI CAATTG 1 cut(s) 1173
MhlI GDGCHC 3 cut(s) 310, 435, 579
MluCI AATT 9 cut(s) 128, 137, 213, 374, 472, 552, 745, 1173, 1301
MlyI GAGTC 1 cut(s) 1045
MroI TCCGGA 1 cut(s) 1284
MroXI GAANNNNTTC 1 cut(s) 1127
MseI TTAA 4 cut(s) 117, 555, 570, 827
MslI CAYNNNNRTG 1 cut(s) 912
MspI CCGG 3 cut(s) 453, 1032, 1285
MspR9I CCNGG 1 cut(s) 1105
MunI CAATTG 1 cut(s) 1173
Mva1269I GAATGC 1 cut(s) 282
MvaI CCWGG 1 cut(s) 1105
MvnI CGCG 1 cut(s) 1215
MwoI GCNNNNNNNGC 3 cut(s) 55, 425, 611
NdeII GATC 6 cut(s) 238, 670, 682, 704, 790, 1292
NlaIII CATG 2 cut(s) 233, 857
NlaIV GGNNCC 4 cut(s) 166, 167, 467, 1111
NmuCI GTSAC 6 cut(s) 232, 260, 1048, 1081, 1135, 1159
NspV TTCGAA 1 cut(s) 979
PceI AGGCCT 1 cut(s) 523
PcsI WCGNNNNNNNCGW 2 cut(s) 316, 1131
PctI GAATGC 1 cut(s) 282
PdmI GAANNNNTTC 1 cut(s) 1127
PfeI GAWTC 3 cut(s) 533, 698, 1263
PflMI CCANNNNNTGG 1 cut(s) 964
PkrI GCNGC 3 cut(s) 352, 920, 973
PleI GAGTC 1 cut(s) 1045
PpsI GAGTC 1 cut(s) 1045
Ppu21I YACGTR 1 cut(s) 1135
PpuMI RGGWCCY 1 cut(s) 165
Psp124BI GAGCTC 2 cut(s) 310, 435
Psp5II RGGWCCY 1 cut(s) 165
Psp6I CCWGG 1 cut(s) 1103
PspGI CCWGG 1 cut(s) 1103
PspN4I GGNNCC 4 cut(s) 166, 167, 467, 1111
PspPI GGNCC 4 cut(s) 165, 299, 465, 875
PspPPI RGGWCCY 1 cut(s) 165
PsrI GAACNNNNNNTAC 2 cut(s) 1115, 1147
PstNI CAGNNNCTG 2 cut(s) 49, 701
RsaI GTAC 1 cut(s) 1232
RsaNI GTAC 1 cut(s) 1231
RseI CAYNNNNRTG 1 cut(s) 912
SacI GAGCTC 2 cut(s) 310, 435
SaqAI TTAA 4 cut(s) 117, 555, 570, 827
SatI GCNGC 3 cut(s) 351, 919, 972
Sau3AI GATC 6 cut(s) 238, 670, 682, 704, 790, 1292
Sau96I GGNCC 4 cut(s) 165, 299, 465, 875
SchI GAGTC 1 cut(s) 1045
ScrFI CCNGG 1 cut(s) 1105
SduI GDGCHC 3 cut(s) 310, 435, 579
SfaNI GCATC 2 cut(s) 494, 1154
SfcI CTRYAG 1 cut(s) 621
SfuI TTCGAA 1 cut(s) 979
SinI GGWCC 4 cut(s) 165, 299, 465, 875
SmiMI CAYNNNNRTG 1 cut(s) 912
SmlI CTYRAG 4 cut(s) 94, 325, 578, 778
SmoI CTYRAG 4 cut(s) 94, 325, 578, 778
Sse9I AATT 9 cut(s) 128, 137, 213, 374, 472, 552, 745, 1173, 1301
SseBI AGGCCT 1 cut(s) 523
SspI AATATT 1 cut(s) 896
SspMI CTAG 2 cut(s) 296, 1007
SstI GAGCTC 2 cut(s) 310, 435
StuI AGGCCT 1 cut(s) 523
StyD4I CCNGG 1 cut(s) 1103
StyI CCWWGG 1 cut(s) 71
TaaI ACNGT 8 cut(s) 82, 223, 291, 692, 739, 769, 817, 862
TaiI ACGT 4 cut(s) 1050, 1128, 1137, 1230
TaqI TCGA 2 cut(s) 741, 979
TasI AATT 9 cut(s) 128, 137, 213, 374, 472, 552, 745, 1173, 1301
TatI WGTACW 1 cut(s) 1230
TfiI GAWTC 3 cut(s) 533, 698, 1263
Tru1I TTAA 4 cut(s) 117, 555, 570, 827
Tru9I TTAA 4 cut(s) 117, 555, 570, 827
TscAI CASTG 9 cut(s) 228, 241, 342, 508, 616, 697, 718, 965, 1164
TseFI GTSAC 6 cut(s) 232, 260, 1048, 1081, 1135, 1159
TseI GCWGC 3 cut(s) 350, 918, 971
Tsp45I GTSAC 6 cut(s) 232, 260, 1048, 1081, 1135, 1159
TspDTI ATGAA 1 cut(s) 218
TspGWI ACGGA 1 cut(s) 308
TspRI CASTG 9 cut(s) 228, 241, 342, 508, 616, 697, 718, 965, 1164
Van91I CCANNNNNTGG 1 cut(s) 964
VpaK11BI GGWCC 4 cut(s) 165, 299, 465, 875
XapI RAATTY 3 cut(s) 128, 213, 1301
XmnI GAANNNNTTC 1 cut(s) 1127
XspI CTAG 2 cut(s) 296, 1007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.