RchiOBHm_Chr4g0429671

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0016314
Physical Location & Seq
Reverse (-)
54427688 .. 54431114
3427 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39844

Sequence Viewer

Length: 1353 bp
ATGGATTGTTGGTCTCCTTCTCTTTCTGTTGACGACGAGTTTCAGAAGCTGGCGTTGCGAGTGAACCCTCCAAGGGTTACAGTTGATAATGGCTCAAGCAGGAAAGCCACTTTGATTAAGGTAGATAGTGCAAATAAGCGTGGGAGTTTGCTAGAGGTGGTTCAGGTTCTGAATGATTTGAATCTCATTATAAGAAGAGCTTACATTTCTTCAGATGGGGAGTGGTTTATGGATGTGTTTCATGTCACTGATCAACAGGGGAATAAGCTCTGTGATGATGGAATAGCTGAGCGCATTCAACAGTCTCTAGGACCAAGAGCTCGGAGCTTCCGTGCCTTGAGAAGTTCAGTGGGCGTCCAAGCTGCCACAGAACACACTACTATTGAATTGACTGGAAGGGACAGGGCAGGCTTGCTTTCAGAGGTTTTTGCTGTTCTTGCTGAGCTCAGATGTAATGTGGTTGCCGGAGAAGTATGGACCCATAATTCAAGAATGGCATCAGTTGTTTACATCACCGATGGGCAAACTGGGAGGCCTATTGATGATTCTGAGCGTCTTTCTGAAATTAAAGAGTTTCTCCTTAATGTGCTCAAGGCAGACAAAGATATGCGCTCTGCCAACACTGCCGTTTCTGTAGGTTCTACACACAAGGAGAGGAGGCTTCACCAGATGATGTATGCAGATCGTGATTATGATCTACACAGTGCAGATTCTGGATCAGCCAGTGATAGAAGCAAACCCCTTGTAACTGTCGAAAATTGTGCAGATAAAGGATATACTGTTGTGAACTTGAGGTGTCCAGATCGCCCTAAGTTGCTCTTTGATACAGTATGCACCTTAACAGATATGCAATATGTGGTATATCATGCAACTGTGATTGCTGAAGGACCAGAGGCTTATCAGGAATATTATATAAGACATACTGATGGCTGCCCTATAAGTTCTGAAGGAGAGAGGCAGAGGGTAATCCATTGCTTGGAGGCTGCTATTCGAAGGCGAACTTCTGAGGGTATAAGACTAGAACTCTGTGGTGATGACAGAGCCGGTCTTCTTTCTGATGTGACTCGCATATTCAGAGAAAACAGCCTTTCAGTCACCCGAGCTGAGGTTATGACCAGGGGTTCCCAAGCTGTGAACGTCTTCTACGTGACTGGTGCATCTGGAAATCCAGTGACGAGTGAGACAATTGAGGCAGTTCGGCAAGAGATTGGCTTGACAATACTGCGCGTAGAAGAAGACATGTACTCAAAATCTCCACCACAGGAGAGTGGGAGATTCTCTTTGGGAAATCTATTCCGGACGAGATCAGAGAAATTCCTTTACAACTTGGGTTTGATAAAGTCGTGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

450

Amino Acids

50.2

Weight (kDa)

5.94

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT_ACR9_3rd PF24931 26 - 102 7.4e-06 ACR9-like, ACT-like domain
ACT PF01842 38 - 88 1.1e-06 ACT domain
ACT PF01842 126 - 173 7.7e-07 ACT domain
ACT PF01842 340 - 399 1.9e-07 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016314)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23320 FvH4_4g23320
malus_domestica MD13G1123000.v1.1 MD16G1123700.v1.1
prunus_persica Prupe.1G221900_v2.0.a1
pyrus_communis pycom13g10740 pycom16g10570
rosa_chinensis RchiOBHm_Chr4g0429671
rosa_laevigata RLG00000007053
rosa_multiflora Rmu_sc0010349.1_g000004
rosa_roxburghii Rroxscaffold_5G00371350
rosa_rugosa Rorug04G0237600
rosa_samantha Rh4AG293700 Rh4DG296600
rosa_wichuraiana Rw4G025420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 191
AccB7I CCANNNNNTGG 1 cut(s) 976
AccII CGCG 1 cut(s) 1227
AccIII TCCGGA 1 cut(s) 1296
AclWI GGATC 1 cut(s) 724
AcsI RAATTY 1 cut(s) 1313
AcuI CTGAAG 3 cut(s) 195, 903, 966
AcyI GRCGYC 1 cut(s) 354
AfaI GTAC 1 cut(s) 1244
AfiI CCNNNNNNNGG 3 cut(s) 73, 976, 1105
AflIII ACRYGT 1 cut(s) 1239
AgsI TTSAA 4 cut(s) 181, 299, 386, 489
AjnI CCWGG 1 cut(s) 1115
Alw21I GWGCWC 3 cut(s) 322, 447, 591
Alw26I GTCTC 3 cut(s) 18, 309, 1175
AlwI GGATC 1 cut(s) 724
AlwNI CAGNNNCTG 3 cut(s) 49, 169, 713
Ama87I CYCGRG 1 cut(s) 1098
Aor13HI TCCGGA 1 cut(s) 1296
AoxI GGCC 1 cut(s) 533
ApeKI GCWGC 3 cut(s) 362, 930, 983
ApoI RAATTY 1 cut(s) 1313
Asp700I GAANNNNTTC 1 cut(s) 1139
AspLEI GCGC 3 cut(s) 294, 612, 1227
AspS9I GGNCC 3 cut(s) 311, 477, 887
AsuHPI GGTGA 4 cut(s) 505, 656, 1043, 1087
AsuII TTCGAA 1 cut(s) 991
AvaI CYCGRG 1 cut(s) 1098
AvaII GGWCC 3 cut(s) 311, 477, 887
BanII GRGCYC 2 cut(s) 322, 447
BbsI GAAGAC 3 cut(s) 1040, 1132, 1242
Bbv12I GWGCWC 3 cut(s) 322, 447, 591
BbvCI CCTCAGC 1 cut(s) 1104
BbvI GCAGC 3 cut(s) 349, 917, 970
BccI CCATC 4 cut(s) 209, 272, 512, 920
BceAI ACGGC 1 cut(s) 611
BcgI CGANNNNNNTGC 2 cut(s) 743, 777
BciT130I CCWGG 1 cut(s) 1117
BclI TGATCA 1 cut(s) 250
BcoDI GTCTC 3 cut(s) 18, 309, 1175
BfaI CTAG 3 cut(s) 152, 308, 1019
BfmI CTRYAG 1 cut(s) 633
BisI GCNGC 3 cut(s) 363, 931, 984
BlpI GCTNAGC 2 cut(s) 288, 441
BlsI GCNGC 3 cut(s) 364, 932, 985
Bme1390I CCNGG 1 cut(s) 1117
Bme18I GGWCC 3 cut(s) 311, 477, 887
BmeT110I CYCGRG 1 cut(s) 1098
BmgT120I GGNCC 3 cut(s) 311, 477, 887
BmiI GGNNCC 2 cut(s) 479, 1123
BmrFI CCNGG 1 cut(s) 1117
BmrI ACTGGG 1 cut(s) 537
BmsI GCATC 2 cut(s) 506, 1166
BmuI ACTGGG 1 cut(s) 537
BpiI GAAGAC 3 cut(s) 1040, 1132, 1242
Bpu10I CCTNAGC 1 cut(s) 1104
Bpu1102I GCTNAGC 2 cut(s) 288, 441
Bpu14I TTCGAA 1 cut(s) 991
BpuEI CTTGAG 4 cut(s) 79, 358, 575, 811
BsaAI YACGTR 1 cut(s) 1147
BsaBI GATNNNNATC 2 cut(s) 180, 693
BsaHI GRCGYC 1 cut(s) 354
BsaI GGTCTC 1 cut(s) 18
BsaJI CCNNGG 2 cut(s) 71, 1116
BsaWI WCCGGW 1 cut(s) 1296
BsaXI ACNNNNNCTCC 2 cut(s) 316, 346
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 976, 1105
Bse118I RCCGGY 1 cut(s) 1043
Bse1I ACTGG 5 cut(s) 397, 532, 723, 1156, 1169
Bse3DI GCAATG 1 cut(s) 970
Bse8I GATNNNNATC 2 cut(s) 180, 693
BseAI TCCGGA 1 cut(s) 1296
BseBI CCWGG 1 cut(s) 1117
BseDI CCNNGG 2 cut(s) 71, 1116
BseGI GGATG 1 cut(s) 238
BseJI GATNNNNATC 2 cut(s) 180, 693
BseLI CCNNNNNNNGG 3 cut(s) 73, 976, 1105
BseMI GCAATG 1 cut(s) 970
BseMII CTCAG 6 cut(s) 279, 432, 460, 540, 996, 1095
BseNI ACTGG 5 cut(s) 397, 532, 723, 1156, 1169
BseRI GAGGAG 1 cut(s) 670
BseXI GCAGC 3 cut(s) 349, 917, 970
BsgI GTGCAG 2 cut(s) 726, 783
Bsh1236I CGCG 1 cut(s) 1227
BshFI GGCC 1 cut(s) 535
BsiHKAI GWGCWC 3 cut(s) 322, 447, 591
BsiHKCI CYCGRG 1 cut(s) 1098
BsiSI CCGG 3 cut(s) 465, 1044, 1297
BslFI GGGAC 1 cut(s) 413
BslI CCNNNNNNNGG 3 cut(s) 73, 976, 1105
BsmAI GTCTC 3 cut(s) 18, 309, 1175
BsmFI GGGAC 1 cut(s) 413
BsmI GAATGC 1 cut(s) 294
BsnI GGCC 1 cut(s) 535
Bso31I GGTCTC 1 cut(s) 18
BsoBI CYCGRG 1 cut(s) 1098
Bsp119I TTCGAA 1 cut(s) 991
Bsp1286I GDGCHC 3 cut(s) 322, 447, 591
Bsp13I TCCGGA 1 cut(s) 1296
Bsp143I GATC 6 cut(s) 250, 682, 694, 716, 802, 1304
Bsp1720I GCTNAGC 2 cut(s) 288, 441
BspANI GGCC 1 cut(s) 535
BspCNI CTCAG 6 cut(s) 280, 433, 459, 541, 997, 1096
BspEI TCCGGA 1 cut(s) 1296
BspFNI CGCG 1 cut(s) 1227
BspLI GGNNCC 2 cut(s) 479, 1123
BspPI GGATC 1 cut(s) 724
BspQI GCTCTTC 1 cut(s) 190
BspT104I TTCGAA 1 cut(s) 991
BspTNI GGTCTC 1 cut(s) 18
BsrDI GCAATG 1 cut(s) 970
BsrFI RCCGGY 1 cut(s) 1043
BsrI ACTGG 5 cut(s) 397, 532, 723, 1156, 1169
BssAI RCCGGY 1 cut(s) 1043
BssECI CCNNGG 2 cut(s) 71, 1116
BssMI GATC 6 cut(s) 250, 682, 694, 716, 802, 1304
BssNI GRCGYC 1 cut(s) 354
BssT1I CCWWGG 1 cut(s) 71
Bst2UI CCWGG 1 cut(s) 1117
Bst4CI ACNGT 7 cut(s) 82, 303, 704, 751, 781, 829, 874
Bst6I CTCTTC 1 cut(s) 190
BstACI GRCGYC 1 cut(s) 354
BstBAI YACGTR 1 cut(s) 1147
BstBI TTCGAA 1 cut(s) 991
BstC8I GCNNGC 3 cut(s) 51, 409, 413
BstDEI CTNAG 7 cut(s) 288, 441, 446, 549, 810, 1005, 1104
BstF5I GGATG 1 cut(s) 238
BstFNI CGCG 1 cut(s) 1227
BstHHI GCGC 3 cut(s) 294, 612, 1227
BstKTI GATC 6 cut(s) 253, 685, 697, 719, 805, 1307
BstMAI GTCTC 3 cut(s) 18, 309, 1175
BstMBI GATC 6 cut(s) 250, 682, 694, 716, 802, 1304
BstMWI GCNNNNNNNGC 3 cut(s) 55, 437, 623
BstNI CCWGG 1 cut(s) 1117
BstNSI RCATGY 1 cut(s) 1243
BstSCI CCNGG 1 cut(s) 1115
BstSFI CTRYAG 1 cut(s) 633
BstUI CGCG 1 cut(s) 1227
BstV1I GCAGC 3 cut(s) 349, 917, 970
BstV2I GAAGAC 3 cut(s) 1040, 1132, 1242
BsuRI GGCC 1 cut(s) 535
BtsCI GGATG 1 cut(s) 238
BtsI GCAGTG 1 cut(s) 621
BtsIMutI CAGTG 6 cut(s) 246, 354, 621, 709, 730, 1176
Cac8I GCNNGC 3 cut(s) 51, 409, 413
CaiI CAGNNNCTG 3 cut(s) 49, 169, 713
CfoI GCGC 3 cut(s) 294, 612, 1227
Cfr10I RCCGGY 1 cut(s) 1043
Cfr13I GGNCC 3 cut(s) 311, 477, 887
CseI GACGC 2 cut(s) 343, 542
Csp6I GTAC 1 cut(s) 1243
CviAII CATG 3 cut(s) 242, 866, 1240
CviQI GTAC 1 cut(s) 1243
DdeI CTNAG 7 cut(s) 288, 441, 446, 549, 810, 1005, 1104
DpnI GATC 6 cut(s) 252, 684, 696, 718, 804, 1306
DpnII GATC 6 cut(s) 250, 682, 694, 716, 802, 1304
Eam1104I CTCTTC 1 cut(s) 190
EarI CTCTTC 1 cut(s) 190
Ecl136II GAGCTC 2 cut(s) 320, 445
Eco130I CCWWGG 1 cut(s) 71
Eco147I AGGCCT 1 cut(s) 535
Eco24I GRGCYC 2 cut(s) 322, 447
Eco31I GGTCTC 1 cut(s) 18
Eco47I GGWCC 3 cut(s) 311, 477, 887
Eco53kI GAGCTC 2 cut(s) 320, 445
Eco57I CTGAAG 3 cut(s) 195, 903, 966
Eco88I CYCGRG 1 cut(s) 1098
EcoICRI GAGCTC 2 cut(s) 320, 445
EcoRII CCWGG 1 cut(s) 1115
EcoT14I CCWWGG 1 cut(s) 71
EcoT38I GRGCYC 2 cut(s) 322, 447
ErhI CCWWGG 1 cut(s) 71
FaeI CATG 3 cut(s) 245, 869, 1243
FalI AAGNNNNNCTT 6 cut(s) 184, 216, 803, 835, 985, 1017
FaqI GGGAC 1 cut(s) 413
FatI CATG 3 cut(s) 241, 865, 1239
FbaI TGATCA 1 cut(s) 250
Fnu4HI GCNGC 3 cut(s) 363, 931, 984
FokI GGATG 1 cut(s) 245
FriOI GRGCYC 2 cut(s) 322, 447
Fsp4HI GCNGC 3 cut(s) 363, 931, 984
FspBI CTAG 3 cut(s) 152, 308, 1019
GlaI GCGC 3 cut(s) 293, 611, 1226
GluI GCNGC 3 cut(s) 363, 931, 984
HaeIII GGCC 1 cut(s) 535
HapII CCGG 3 cut(s) 465, 1044, 1297
HgaI GACGC 2 cut(s) 343, 542
HhaI GCGC 3 cut(s) 294, 612, 1227
Hin1I GRCGYC 1 cut(s) 354
Hin1II CATG 3 cut(s) 245, 869, 1243
Hin6I GCGC 3 cut(s) 292, 610, 1225
HinP1I GCGC 3 cut(s) 292, 610, 1225
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HinfI GANTC 5 cut(s) 181, 545, 710, 1063, 1275
HpaII CCGG 3 cut(s) 465, 1044, 1297
HphI GGTGA 4 cut(s) 505, 656, 1043, 1087
Hpy166II GTNNAC 5 cut(s) 31, 64, 508, 787, 1135
Hpy188III TCNNGA 8 cut(s) 489, 686, 714, 800, 902, 1161, 1297, 1350
Hpy8I GTNNAC 5 cut(s) 31, 64, 508, 787, 1135
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 5 cut(s) 27, 390, 878, 941, 987
HpyCH4III ACNGT 7 cut(s) 82, 303, 704, 751, 781, 829, 874
HpyCH4IV ACGT 2 cut(s) 1137, 1146
HpyCH4V TGCA 8 cut(s) 131, 680, 707, 764, 834, 850, 869, 1157
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 437, 623
HpyF3I CTNAG 7 cut(s) 288, 441, 446, 549, 810, 1005, 1104
HpySE526I ACGT 2 cut(s) 1137, 1146
Hsp92I GRCGYC 1 cut(s) 354
Hsp92II CATG 3 cut(s) 245, 869, 1243
HspAI GCGC 3 cut(s) 292, 610, 1225
Kpn2I TCCGGA 1 cut(s) 1296
Ksp22I TGATCA 1 cut(s) 250
Kzo9I GATC 6 cut(s) 250, 682, 694, 716, 802, 1304
LguI GCTCTTC 1 cut(s) 190
LmnI GCTCC 1 cut(s) 324
Lsp1109I GCAGC 3 cut(s) 349, 917, 970
LweI GCATC 2 cut(s) 506, 1166
MaeI CTAG 3 cut(s) 152, 308, 1019
MaeII ACGT 2 cut(s) 1137, 1146
MaeIII GTNAC 7 cut(s) 76, 244, 745, 1060, 1093, 1147, 1171
MalI GATC 6 cut(s) 252, 684, 696, 718, 804, 1306
MboI GATC 6 cut(s) 250, 682, 694, 716, 802, 1304
MboII GAAGA 6 cut(s) 201, 207, 1040, 1132, 1244, 1247
MfeI CAATTG 1 cut(s) 1185
MhlI GDGCHC 3 cut(s) 322, 447, 591
MluCI AATT 6 cut(s) 386, 484, 564, 757, 1185, 1313
MlyI GAGTC 1 cut(s) 1057
MroI TCCGGA 1 cut(s) 1296
MroXI GAANNNNTTC 1 cut(s) 1139
MseI TTAA 4 cut(s) 117, 567, 582, 839
MslI CAYNNNNRTG 1 cut(s) 924
MspI CCGG 3 cut(s) 465, 1044, 1297
MspR9I CCNGG 1 cut(s) 1117
MunI CAATTG 1 cut(s) 1185
Mva1269I GAATGC 1 cut(s) 294
MvaI CCWGG 1 cut(s) 1117
MvnI CGCG 1 cut(s) 1227
MwoI GCNNNNNNNGC 3 cut(s) 55, 437, 623
NdeII GATC 6 cut(s) 250, 682, 694, 716, 802, 1304
NlaIII CATG 3 cut(s) 245, 869, 1243
NlaIV GGNNCC 2 cut(s) 479, 1123
NmuCI GTSAC 5 cut(s) 244, 1060, 1093, 1147, 1171
NspI RCATGY 1 cut(s) 1243
NspV TTCGAA 1 cut(s) 991
PceI AGGCCT 1 cut(s) 535
PciI ACATGT 1 cut(s) 1239
PciSI GCTCTTC 1 cut(s) 190
PcsI WCGNNNNNNNCGW 2 cut(s) 328, 1143
PctI GAATGC 1 cut(s) 294
PdmI GAANNNNTTC 1 cut(s) 1139
PfeI GAWTC 4 cut(s) 181, 545, 710, 1275
PflMI CCANNNNNTGG 1 cut(s) 976
PkrI GCNGC 3 cut(s) 364, 932, 985
PleI GAGTC 1 cut(s) 1057
PpsI GAGTC 1 cut(s) 1057
Ppu21I YACGTR 1 cut(s) 1147
PscI ACATGT 1 cut(s) 1239
PsiI TTATAA 1 cut(s) 191
Psp124BI GAGCTC 2 cut(s) 322, 447
Psp6I CCWGG 1 cut(s) 1115
PspGI CCWGG 1 cut(s) 1115
PspN4I GGNNCC 2 cut(s) 479, 1123
PspPI GGNCC 3 cut(s) 311, 477, 887
PsrI GAACNNNNNNTAC 2 cut(s) 1127, 1159
PstNI CAGNNNCTG 3 cut(s) 49, 169, 713
RsaI GTAC 1 cut(s) 1244
RsaNI GTAC 1 cut(s) 1243
RseI CAYNNNNRTG 1 cut(s) 924
SacI GAGCTC 2 cut(s) 322, 447
SapI GCTCTTC 1 cut(s) 190
SaqAI TTAA 4 cut(s) 117, 567, 582, 839
SatI GCNGC 3 cut(s) 363, 931, 984
Sau3AI GATC 6 cut(s) 250, 682, 694, 716, 802, 1304
Sau96I GGNCC 3 cut(s) 311, 477, 887
SchI GAGTC 1 cut(s) 1057
ScrFI CCNGG 1 cut(s) 1117
SduI GDGCHC 3 cut(s) 322, 447, 591
SfaNI GCATC 2 cut(s) 506, 1166
SfcI CTRYAG 1 cut(s) 633
SfuI TTCGAA 1 cut(s) 991
SinI GGWCC 3 cut(s) 311, 477, 887
SmiMI CAYNNNNRTG 1 cut(s) 924
SmlI CTYRAG 4 cut(s) 94, 337, 590, 790
SmoI CTYRAG 4 cut(s) 94, 337, 590, 790
Sse9I AATT 6 cut(s) 386, 484, 564, 757, 1185, 1313
SseBI AGGCCT 1 cut(s) 535
SspI AATATT 1 cut(s) 908
SspMI CTAG 3 cut(s) 152, 308, 1019
SstI GAGCTC 2 cut(s) 322, 447
StuI AGGCCT 1 cut(s) 535
StyD4I CCNGG 1 cut(s) 1115
StyI CCWWGG 1 cut(s) 71
TaaI ACNGT 7 cut(s) 82, 303, 704, 751, 781, 829, 874
TaiI ACGT 2 cut(s) 1140, 1149
TaqI TCGA 2 cut(s) 753, 991
TasI AATT 6 cut(s) 386, 484, 564, 757, 1185, 1313
TatI WGTACW 1 cut(s) 1242
TfiI GAWTC 4 cut(s) 181, 545, 710, 1275
Tru1I TTAA 4 cut(s) 117, 567, 582, 839
Tru9I TTAA 4 cut(s) 117, 567, 582, 839
TscAI CASTG 6 cut(s) 253, 354, 628, 709, 730, 1176
TseFI GTSAC 5 cut(s) 244, 1060, 1093, 1147, 1171
TseI GCWGC 3 cut(s) 362, 930, 983
Tsp45I GTSAC 5 cut(s) 244, 1060, 1093, 1147, 1171
TspDTI ATGAA 1 cut(s) 230
TspGWI ACGGA 1 cut(s) 320
TspRI CASTG 6 cut(s) 253, 354, 628, 709, 730, 1176
Van91I CCANNNNNTGG 1 cut(s) 976
VpaK11BI GGWCC 3 cut(s) 311, 477, 887
XapI RAATTY 1 cut(s) 1313
XceI RCATGY 1 cut(s) 1243
XmnI GAANNNNTTC 1 cut(s) 1139
XspI CTAG 3 cut(s) 152, 308, 1019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.