MD13G1123000.v1.1

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016314
Physical Location & Seq
Forward (+)
9122112 .. 9125415
3304 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1123000.v1.1.491

Sequence Viewer

Length: 1350 bp
ATGGATTGTTGGTCCCCTTCTCTCGCCGTCGACGACGAGTTCGAGAAGCTCGCCATGAGAATCAACCCCCCGATGGTTACAGTTGATAATGCATCGGGCAGGAAAGCCACTCTGATTAAGGTAGACAGTGCGAATAAGCGTGGGAGTTTGCTGGAGGTGGTTCAGGTTCTGAATGATTTGAATCTCATTATAAGAAGAGCTTATATTTCTTCTGATGGCGAATGGTTCATGGATGTTTTTCATGTCACTGACCAACAGGGGAATAAGCTCTCTGATGACGGAATCGCGGAACGCATTCAACAGTCACTGGGACCAAGAGCACGAAGCTTCCGATCCTTGAGAAGGTCCGTGGGTGTCCAAGCTGCCGCAGAACACACTACTATTGAATTGACTGGAAGGGACAGGCCAGGCTTGCTTTCTGAAGTTTTTGCTGTTCTTTCGAACCTCAAATGCAATGTGGTTGCTGGAGAAGTCTGGACTCACAATTCAAGAATGGCATCAGTTGTGTACATCACCGACGAGGAAACTGGGAGGCCCATTGATGATCCTGAGCATCTTACCAAGATCAAACAGCTTCTCCTTTATGTGCTTAAGGGGGACAGAGATAAGCGCAGTGCCAACACCGCTGTTTCTGTTGGTTCCACACATAAGGAGAGGAGGCTTCACCAGATGATGTACGCTGATCGTGATTATGACATGGATAATGCGGACTCTGTAGCCAGTGACCGTAGCAAACCACTTGTAACCGTTGAAAATTGTGTCGATAAAGGATATACTGTTGTGAACTTGAGGTGTCCAGACCGTCCTAAGTTGCTCTTTGACACGGTGTGCACTTTAACAGATATGGAGTATGTGGTTTATCATGCAACTGTGATTGCTGAAGGACCAGAGGCTTATCAGGAATATTATATAAGACATACCGACGGCTGCCCTATTAGTTCTGAAGGAGAAAGGCAGAGGGTTATCCATTGCTTGGAAGCTGCTATTAGACGACGGACTTCTGAGGGTATAAGACTAGAGCTTTGTGGTGATGACAGAGCCGGCCTTCTTTCTGATGTGACACGCATATTTAGAGAAAACGGTCTTTCAGTCACCCGAGCTGAGGTTACAACCAGGGGCTCCCAAGCTGTGAATGTATTCTATGTGACCGATGCATCTGGAAATCAAGTCAAGAGCGAGACAATTGAAGCAGTTCGGCAAGAGATTGGCTTGGCATTACTGAGTCTGCAAGACGACTTCTGTTCAAAATCTCCACCGCAGGAGAGTGGGAGATTCTCTTTGGGCAATCTCTTTCGCACTAGGTCGGAGAAGTTCCTTTACAACCTGGGTTTGATAAAGTCATGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

450

Amino Acids

50.24

Weight (kDa)

5.83

Isoelectric Point (pI)

39.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 38 - 88 1.1e-06 ACT domain
ACT PF01842 126 - 192 1.6e-08 ACT domain
ACT PF01842 339 - 396 2.1e-09 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016314)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23320 FvH4_4g23320
malus_domestica MD13G1123000.v1.1 MD16G1123700.v1.1
prunus_persica Prupe.1G221900_v2.0.a1
pyrus_communis pycom13g10740 pycom16g10570
rosa_chinensis RchiOBHm_Chr4g0429671
rosa_laevigata RLG00000007053
rosa_multiflora Rmu_sc0010349.1_g000004
rosa_roxburghii Rroxscaffold_5G00371350
rosa_rugosa Rorug04G0237600
rosa_samantha Rh4AG293700 Rh4DG296600
rosa_wichuraiana Rw4G025420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 191
AccB7I CCANNNNNTGG 1 cut(s) 973
AccI GTMKAC 2 cut(s) 30, 123
AccII CGCG 1 cut(s) 287
AciI CCGC 5 cut(s) 287, 366, 624, 707, 1256
AclWI GGATC 2 cut(s) 327, 539
AcuI CTGAAG 3 cut(s) 441, 900, 963
AdeI CACNNNGTG 1 cut(s) 828
AfaI GTAC 2 cut(s) 509, 677
AfiI CCNNNNNNNGG 4 cut(s) 73, 342, 973, 1102
AflII CTTAAG 1 cut(s) 590
AgsI TTSAA 7 cut(s) 181, 299, 386, 489, 752, 1187, 1245
AjnI CCWGG 3 cut(s) 406, 1112, 1323
Alw21I GWGCWC 2 cut(s) 322, 833
Alw26I GTCTC 1 cut(s) 1172
Alw44I GTGCAC 1 cut(s) 829
AlwI GGATC 2 cut(s) 327, 539
AlwNI CAGNNNCTG 2 cut(s) 169, 307
Ama87I CYCGRG 1 cut(s) 1095
AoxI GGCC 3 cut(s) 404, 533, 1042
ApaLI GTGCAC 1 cut(s) 829
ApeKI GCWGC 3 cut(s) 362, 927, 980
ArsI GACNNNNNNTTYG 4 cut(s) 23, 55, 1005, 1037
Asp700I GAANNNNTTC 3 cut(s) 294, 1136, 1191
AspLEI GCGC 1 cut(s) 612
AspS9I GGNCC 5 cut(s) 12, 311, 345, 534, 884
AsuHPI GGTGA 4 cut(s) 505, 656, 1040, 1084
AsuII TTCGAA 1 cut(s) 440
AvaI CYCGRG 1 cut(s) 1095
AvaII GGWCC 4 cut(s) 12, 311, 345, 884
BaeGI GKGCMC 1 cut(s) 833
BanII GRGCYC 1 cut(s) 1121
BarI GAAGNNNNNNTAC 2 cut(s) 1301, 1333
Bbv12I GWGCWC 2 cut(s) 322, 833
BbvCI CCTCAGC 1 cut(s) 1101
BbvI GCAGC 3 cut(s) 349, 914, 967
BccI CCATC 2 cut(s) 67, 209
BceAI ACGGC 2 cut(s) 11, 940
BciT130I CCWGG 3 cut(s) 408, 1114, 1325
BcoDI GTCTC 1 cut(s) 1172
BfaI CTAG 2 cut(s) 1016, 1299
BfmI CTRYAG 1 cut(s) 714
BfrI CTTAAG 1 cut(s) 590
BisI GCNGC 4 cut(s) 363, 366, 928, 981
BlsI GCNGC 4 cut(s) 364, 367, 929, 982
Bme1390I CCNGG 3 cut(s) 408, 1114, 1325
Bme18I GGWCC 4 cut(s) 12, 311, 345, 884
BmeT110I CYCGRG 1 cut(s) 1095
BmgT120I GGNCC 5 cut(s) 12, 311, 345, 534, 884
BmiI GGNNCC 4 cut(s) 14, 312, 640, 1120
BmrFI CCNGG 3 cut(s) 408, 1114, 1325
BmrI ACTGGG 2 cut(s) 317, 537
BmsI GCATC 5 cut(s) 101, 506, 562, 1141, 1163
BmuI ACTGGG 2 cut(s) 317, 537
BpmI CTGGAG 2 cut(s) 173, 486
Bpu10I CCTNAGC 2 cut(s) 549, 1101
Bpu14I TTCGAA 1 cut(s) 440
BpuEI CTTGAG 2 cut(s) 358, 808
BsaBI GATNNNNATC 1 cut(s) 180
BsaJI CCNNGG 3 cut(s) 348, 1113, 1324
BsaXI ACNNNNNCTCC 2 cut(s) 561, 591
Bsc4I CCNNNNNNNGG 4 cut(s) 73, 342, 973, 1102
Bse118I RCCGGY 1 cut(s) 1040
Bse1I ACTGG 4 cut(s) 312, 397, 532, 720
Bse3DI GCAATG 2 cut(s) 460, 967
Bse8I GATNNNNATC 1 cut(s) 180
BseBI CCWGG 3 cut(s) 408, 1114, 1325
BseDI CCNNGG 3 cut(s) 348, 1113, 1324
BseGI GGATG 1 cut(s) 238
BseJI GATNNNNATC 1 cut(s) 180
BseLI CCNNNNNNNGG 4 cut(s) 73, 342, 973, 1102
BseMI GCAATG 2 cut(s) 460, 967
BseMII CTCAG 4 cut(s) 540, 993, 1092, 1211
BseNI ACTGG 4 cut(s) 312, 397, 532, 720
BseRI GAGGAG 1 cut(s) 670
BseSI GKGCMC 1 cut(s) 833
BseXI GCAGC 3 cut(s) 349, 914, 967
Bsh1236I CGCG 1 cut(s) 287
BshFI GGCC 3 cut(s) 406, 535, 1044
BsiHKAI GWGCWC 2 cut(s) 322, 833
BsiHKCI CYCGRG 1 cut(s) 1095
BsiSI CCGG 1 cut(s) 1041
BslFI GGGAC 3 cut(s) 324, 413, 611
BslI CCNNNNNNNGG 4 cut(s) 73, 342, 973, 1102
BsmAI GTCTC 1 cut(s) 1172
BsmFI GGGAC 3 cut(s) 324, 413, 611
BsmI GAATGC 1 cut(s) 294
BsnI GGCC 3 cut(s) 406, 535, 1044
BsoBI CYCGRG 1 cut(s) 1095
Bsp119I TTCGAA 1 cut(s) 440
Bsp1286I GDGCHC 3 cut(s) 322, 833, 1121
Bsp1407I TGTACA 1 cut(s) 507
Bsp143I GATC 4 cut(s) 332, 544, 564, 682
BspACI CCGC 5 cut(s) 287, 366, 624, 707, 1256
BspANI GGCC 3 cut(s) 406, 535, 1044
BspCNI CTCAG 4 cut(s) 541, 994, 1093, 1212
BspFNI CGCG 1 cut(s) 287
BspLI GGNNCC 4 cut(s) 14, 312, 640, 1120
BspPI GGATC 2 cut(s) 327, 539
BspQI GCTCTTC 1 cut(s) 190
BspT104I TTCGAA 1 cut(s) 440
BspTI CTTAAG 1 cut(s) 590
BsrDI GCAATG 2 cut(s) 460, 967
BsrFI RCCGGY 1 cut(s) 1040
BsrGI TGTACA 1 cut(s) 507
BsrI ACTGG 4 cut(s) 312, 397, 532, 720
BssAI RCCGGY 1 cut(s) 1040
BssECI CCNNGG 3 cut(s) 348, 1113, 1324
BssMI GATC 4 cut(s) 332, 544, 564, 682
Bst2UI CCWGG 3 cut(s) 408, 1114, 1325
Bst6I CTCTTC 1 cut(s) 190
BstAFI CTTAAG 1 cut(s) 590
BstAUI TGTACA 1 cut(s) 507
BstBI TTCGAA 1 cut(s) 440
BstC8I GCNNGC 3 cut(s) 51, 413, 1042
BstDEI CTNAG 5 cut(s) 549, 807, 1002, 1101, 1220
BstDSI CCRYGG 1 cut(s) 348
BstENI CCTNNNNNAGG 1 cut(s) 340
BstF5I GGATG 1 cut(s) 238
BstFNI CGCG 1 cut(s) 287
BstHHI GCGC 1 cut(s) 612
BstKTI GATC 4 cut(s) 335, 547, 567, 685
BstMAI GTCTC 1 cut(s) 1172
BstMBI GATC 4 cut(s) 332, 544, 564, 682
BstMWI GCNNNNNNNGC 2 cut(s) 412, 623
BstNI CCWGG 3 cut(s) 408, 1114, 1325
BstSCI CCNGG 3 cut(s) 406, 1112, 1323
BstSFI CTRYAG 1 cut(s) 714
BstSLI GKGCMC 1 cut(s) 833
BstUI CGCG 1 cut(s) 287
BstV1I GCAGC 3 cut(s) 349, 914, 967
BsuRI GGCC 3 cut(s) 406, 535, 1044
BtgI CCRYGG 1 cut(s) 348
BtsCI GGATG 1 cut(s) 238
BtsI GCAGTG 1 cut(s) 619
BtsIMutI CAGTG 5 cut(s) 133, 246, 305, 619, 727
Cac8I GCNNGC 3 cut(s) 51, 413, 1042
CaiI CAGNNNCTG 2 cut(s) 169, 307
CfoI GCGC 1 cut(s) 612
Cfr10I RCCGGY 1 cut(s) 1040
Cfr13I GGNCC 5 cut(s) 12, 311, 345, 534, 884
Csp6I GTAC 2 cut(s) 508, 676
CviAII CATG 6 cut(s) 55, 229, 242, 697, 863, 1341
CviQI GTAC 2 cut(s) 508, 676
DdeI CTNAG 5 cut(s) 549, 807, 1002, 1101, 1220
DpnI GATC 4 cut(s) 334, 546, 566, 684
DpnII GATC 4 cut(s) 332, 544, 564, 682
DraIII CACNNNGTG 1 cut(s) 828
Eam1104I CTCTTC 1 cut(s) 190
EarI CTCTTC 1 cut(s) 190
Eco24I GRGCYC 1 cut(s) 1121
Eco47I GGWCC 4 cut(s) 12, 311, 345, 884
Eco57I CTGAAG 3 cut(s) 441, 900, 963
Eco88I CYCGRG 1 cut(s) 1095
EcoNI CCTNNNNNAGG 1 cut(s) 340
EcoRII CCWGG 3 cut(s) 406, 1112, 1323
EcoT22I ATGCAT 2 cut(s) 94, 1156
EcoT38I GRGCYC 1 cut(s) 1121
FaeI CATG 6 cut(s) 58, 232, 245, 700, 866, 1344
FalI AAGNNNNNCTT 4 cut(s) 184, 216, 800, 832
FaqI GGGAC 3 cut(s) 324, 413, 611
FatI CATG 6 cut(s) 54, 228, 241, 696, 862, 1340
FblI GTMKAC 2 cut(s) 30, 123
Fnu4HI GCNGC 4 cut(s) 363, 366, 928, 981
FokI GGATG 1 cut(s) 245
FriOI GRGCYC 1 cut(s) 1121
Fsp4HI GCNGC 4 cut(s) 363, 366, 928, 981
FspBI CTAG 2 cut(s) 1016, 1299
GlaI GCGC 1 cut(s) 611
GluI GCNGC 4 cut(s) 363, 366, 928, 981
GsuI CTGGAG 2 cut(s) 173, 486
HaeIII GGCC 3 cut(s) 406, 535, 1044
HapII CCGG 1 cut(s) 1041
HhaI GCGC 1 cut(s) 612
Hin1II CATG 6 cut(s) 58, 232, 245, 700, 866, 1344
Hin6I GCGC 1 cut(s) 610
HinP1I GCGC 1 cut(s) 610
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HindIII AAGCTT 1 cut(s) 325
HinfI GANTC 7 cut(s) 60, 181, 282, 478, 710, 1222, 1272
HpaII CCGG 1 cut(s) 1041
HphI GGTGA 4 cut(s) 505, 656, 1040, 1084
Hpy166II GTNNAC 5 cut(s) 31, 124, 508, 784, 831
Hpy8I GTNNAC 5 cut(s) 31, 124, 508, 784, 831
Hpy99I CGWCG 6 cut(s) 32, 35, 38, 521, 926, 996
HpyAV CCTTC 6 cut(s) 27, 336, 390, 875, 938, 1055
HpyCH4V TGCA 6 cut(s) 92, 453, 831, 866, 1154, 1228
HpyF10VI GCNNNNNNNGC 2 cut(s) 412, 623
HpyF3I CTNAG 5 cut(s) 549, 807, 1002, 1101, 1220
Hsp92II CATG 6 cut(s) 58, 232, 245, 700, 866, 1344
HspAI GCGC 1 cut(s) 610
KroI GCCGGC 1 cut(s) 1040
KroNI GCCGGC 1 cut(s) 1042
Kzo9I GATC 4 cut(s) 332, 544, 564, 682
LguI GCTCTTC 1 cut(s) 190
LmnI GCTCC 1 cut(s) 1124
Lsp1109I GCAGC 3 cut(s) 349, 914, 967
LweI GCATC 5 cut(s) 101, 506, 562, 1141, 1163
MaeI CTAG 2 cut(s) 1016, 1299
MaeIII GTNAC 9 cut(s) 76, 244, 303, 722, 742, 1057, 1090, 1105, 1144
MalI GATC 4 cut(s) 334, 546, 566, 684
MboI GATC 4 cut(s) 332, 544, 564, 682
MboII GAAGA 2 cut(s) 201, 207
MfeI CAATTG 1 cut(s) 1182
MhlI GDGCHC 3 cut(s) 322, 833, 1121
MluCI AATT 4 cut(s) 386, 484, 754, 1182
MlyI GAGTC 3 cut(s) 472, 704, 1231
MmeI TCCRAC 1 cut(s) 1284
Mph1103I ATGCAT 2 cut(s) 94, 1156
MroNI GCCGGC 1 cut(s) 1040
MroXI GAANNNNTTC 3 cut(s) 294, 1136, 1191
MseI TTAA 3 cut(s) 117, 591, 836
MspA1I CMGCKG 1 cut(s) 626
MspCI CTTAAG 1 cut(s) 590
MspI CCGG 1 cut(s) 1041
MspR9I CCNGG 3 cut(s) 408, 1114, 1325
MunI CAATTG 1 cut(s) 1182
Mva1269I GAATGC 1 cut(s) 294
MvaI CCWGG 3 cut(s) 408, 1114, 1325
MvnI CGCG 1 cut(s) 287
MwoI GCNNNNNNNGC 2 cut(s) 412, 623
NaeI GCCGGC 1 cut(s) 1042
NdeII GATC 4 cut(s) 332, 544, 564, 682
NgoMIV GCCGGC 1 cut(s) 1040
NlaIII CATG 6 cut(s) 58, 232, 245, 700, 866, 1344
NlaIV GGNNCC 4 cut(s) 14, 312, 640, 1120
NmuCI GTSAC 6 cut(s) 244, 303, 722, 1057, 1090, 1144
NsiI ATGCAT 2 cut(s) 94, 1156
NspV TTCGAA 1 cut(s) 440
PciSI GCTCTTC 1 cut(s) 190
PcsI WCGNNNNNNNCGW 3 cut(s) 30, 39, 328
PctI GAATGC 1 cut(s) 294
PdiI GCCGGC 1 cut(s) 1042
PdmI GAANNNNTTC 3 cut(s) 294, 1136, 1191
PfeI GAWTC 4 cut(s) 60, 181, 282, 1272
PflMI CCANNNNNTGG 1 cut(s) 973
PkrI GCNGC 4 cut(s) 364, 367, 929, 982
PleI GAGTC 3 cut(s) 472, 704, 1230
PpsI GAGTC 3 cut(s) 472, 704, 1230
PsiI TTATAA 1 cut(s) 191
Psp6I CCWGG 3 cut(s) 406, 1112, 1323
PspGI CCWGG 3 cut(s) 406, 1112, 1323
PspN4I GGNNCC 4 cut(s) 14, 312, 640, 1120
PspPI GGNCC 5 cut(s) 12, 311, 345, 534, 884
PstNI CAGNNNCTG 2 cut(s) 169, 307
RsaI GTAC 2 cut(s) 509, 677
RsaNI GTAC 2 cut(s) 508, 676
SalI GTCGAC 1 cut(s) 29
SapI GCTCTTC 1 cut(s) 190
SaqAI TTAA 3 cut(s) 117, 591, 836
SatI GCNGC 4 cut(s) 363, 366, 928, 981
Sau3AI GATC 4 cut(s) 332, 544, 564, 682
Sau96I GGNCC 5 cut(s) 12, 311, 345, 534, 884
SchI GAGTC 3 cut(s) 472, 704, 1231
ScrFI CCNGG 3 cut(s) 408, 1114, 1325
SduI GDGCHC 3 cut(s) 322, 833, 1121
SfaNI GCATC 5 cut(s) 101, 506, 562, 1141, 1163
SfcI CTRYAG 1 cut(s) 714
SfuI TTCGAA 1 cut(s) 440
SgrDI CGTCGACG 1 cut(s) 29
SinI GGWCC 4 cut(s) 12, 311, 345, 884
SmlI CTYRAG 3 cut(s) 337, 590, 787
SmoI CTYRAG 3 cut(s) 337, 590, 787
Sse9I AATT 4 cut(s) 386, 484, 754, 1182
SsiI CCGC 5 cut(s) 287, 366, 624, 707, 1256
SspI AATATT 1 cut(s) 905
SspMI CTAG 2 cut(s) 1016, 1299
StyD4I CCNGG 3 cut(s) 406, 1112, 1323
TaqI TCGA 4 cut(s) 30, 42, 440, 762
TaqII GACCGA 1 cut(s) 1163
TasI AATT 4 cut(s) 386, 484, 754, 1182
TatI WGTACW 1 cut(s) 507
TauI GCSGC 1 cut(s) 368
TfiI GAWTC 4 cut(s) 60, 181, 282, 1272
Tru1I TTAA 3 cut(s) 117, 591, 836
Tru9I TTAA 3 cut(s) 117, 591, 836
TscAI CASTG 5 cut(s) 133, 253, 312, 619, 727
TseFI GTSAC 6 cut(s) 244, 303, 722, 1057, 1090, 1144
TseI GCWGC 3 cut(s) 362, 927, 980
Tsp45I GTSAC 6 cut(s) 244, 303, 722, 1057, 1090, 1144
TspDTI ATGAA 2 cut(s) 217, 230
TspGWI ACGGA 3 cut(s) 294, 337, 1009
TspRI CASTG 5 cut(s) 133, 253, 312, 619, 727
Van91I CCANNNNNTGG 1 cut(s) 973
Vha464I CTTAAG 1 cut(s) 590
VneI GTGCAC 1 cut(s) 829
VpaK11BI GGWCC 4 cut(s) 12, 311, 345, 884
XagI CCTNNNNNAGG 1 cut(s) 340
XmiI GTMKAC 2 cut(s) 30, 123
XmnI GAANNNNTTC 3 cut(s) 294, 1136, 1191
XspI CTAG 2 cut(s) 1016, 1299
Zsp2I ATGCAT 2 cut(s) 94, 1156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.