Rh4AG293700

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0016314
Physical Location & Seq
Reverse (-)
60712980 .. 60715489
2510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG293700.1

Sequence Viewer

Length: 1455 bp
ATGGATTGTTGGTCTCCTTCTCTTGCTGTTGACGACGAGTTTCAGAAGCTGGCGTTGCGAGTGAACCCTCCAAGGGTTACAGTTGATAATGGCTCAAGCAGGAAAGCCACTTTGATTAAGGTGATTGAATTTCTTGAATTGTCTCTGCAAGCATTTCAAAAAGGGTCGCCCTCTGAACATTTGGTTCTTTGTCTCTTTCTTTTTTTGGGGAAATTTTTACAGGTAGATAGTGCAAATAAGCGTGGGAGTTTGCTAGAGGTGGTTCAGGTTCTGAATGATTTGAATCTCATTATAAGAAGAGCTTACATTTCTTCAGATGGGGAGTGGTTTATGGATGTGTTTCATGTCACTGATCAACAGGGGAATAAGCTCTGTGATGATGGAATAGCTGAGCGAATTCAACAGTCTCTAGGACCAAGAGCTCGGAGCTTCCGTGCCTTGAGAAGTTCAGTGGGCGTCCAAGCTGCCACAGAACACACTACTATTGAATTGACTGGAAGGGACAGGGCAGGCTTGCTTTCAGAGGTTTTTGCTGTTCTTGCTGAGCTCAGATGTAATGTGGTTGCCGGAGAAGTATGGACCCATAATTCAAGAATGGCATCAGTTGTTTACATCACCGATGGGCAAACTGGGAGGCCTATTGATGATTCTGAGCGTCTTTCTGAAATTAAAGAGTTTCTCCTTAATGTGCTCAAGGCAGACAAAGATATGCGCTCTGCCAACACCGCCGTTTCTGTAGGTTCTACACACAAGGAGAGGAGGCTTCACCAGATGATGTATGCAGATCGTGATTATGATCTACACAGTGCAGATTCTGGATCAGCCAGTGATAGAAGCAAACCCTTTGTAACTGTCGAAAATTGTGCAGATAAAGGATATACTGTTGTGAACTTGAGGTGTCCAGATCGTCCTAAGTTGCTCTTTGATACAGTATGCACCTTAACAGATATGCAATATGTGGTATATCATGCAACTGTGATTGCTGAAGGACCAGAGGCTTATCAGGAATATTATATAAGACATACTGATGGCTGCCCTATAAGTTCTGAAGGAGAGAGGCAGAGGGTAATCCATTGCTTGGAGGCTGCTATTCGAAGGCGAACTTCTGAGGGTATAAGACTAGAACTCTGTGGTGATGACAGAGCCGGTCTTCTTTCTGACGTGACTCGCATATTCAGAGAAAACAGCCTTTCAGTCACCCGAGCTGAGGTTATGACCAGGGGTTCCCAAGCTGTGAACGTCTTCTACGTGACTGGTGCATCTGGAAATCCAGTGACGAGTGAGACAATTGAGGCAGTTCGGCAAGAGATTGGCTTGACAATACTGCACGTAGAAGAAGACATGTACTCAAAATCTCCACCACAGGAGAGTGGGAGATTCTCTTTGGGAAATCTATTCCGGACGAGATCAGAGAAATTCCTTTACAACTTGGGTTTGATAAAGTCGTGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

484

Amino Acids

54.08

Weight (kDa)

5.85

Isoelectric Point (pI)

43.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 73 - 122 2.1e-06 ACT domain
ACT PF01842 160 - 207 8.5e-07 ACT domain
ACT PF01842 374 - 433 2.2e-07 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016314)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23320 FvH4_4g23320
malus_domestica MD13G1123000.v1.1 MD16G1123700.v1.1
prunus_persica Prupe.1G221900_v2.0.a1
pyrus_communis pycom13g10740 pycom16g10570
rosa_chinensis RchiOBHm_Chr4g0429671
rosa_laevigata RLG00000007053
rosa_multiflora Rmu_sc0010349.1_g000004
rosa_roxburghii Rroxscaffold_5G00371350
rosa_rugosa Rorug04G0237600
rosa_samantha Rh4AG293700 Rh4DG296600
rosa_wichuraiana Rw4G025420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 293
AccB7I CCANNNNNTGG 1 cut(s) 1078
AccIII TCCGGA 1 cut(s) 1398
AciI CCGC 1 cut(s) 726
AclWI GGATC 1 cut(s) 826
AcsI RAATTY 4 cut(s) 128, 212, 396, 1415
AcuI CTGAAG 3 cut(s) 297, 1005, 1068
AcyI GRCGYC 1 cut(s) 456
AfaI GTAC 1 cut(s) 1346
AfiI CCNNNNNNNGG 3 cut(s) 73, 1078, 1207
AflIII ACRYGT 1 cut(s) 1341
AgsI TTSAA 7 cut(s) 128, 137, 158, 283, 401, 488, 591
AjiI CACGTC 1 cut(s) 1162
AjnI CCWGG 1 cut(s) 1217
Alw21I GWGCWC 3 cut(s) 424, 549, 693
Alw26I GTCTC 5 cut(s) 18, 147, 197, 411, 1277
AlwI GGATC 1 cut(s) 826
AlwNI CAGNNNCTG 3 cut(s) 49, 271, 815
Ama87I CYCGRG 1 cut(s) 1200
Aor13HI TCCGGA 1 cut(s) 1398
AoxI GGCC 1 cut(s) 635
ApeKI GCWGC 3 cut(s) 464, 1032, 1085
ApoI RAATTY 4 cut(s) 128, 212, 396, 1415
Asp700I GAANNNNTTC 1 cut(s) 1241
AspLEI GCGC 1 cut(s) 714
AspS9I GGNCC 3 cut(s) 413, 579, 989
AsuHPI GGTGA 5 cut(s) 133, 607, 758, 1145, 1189
AsuII TTCGAA 1 cut(s) 1093
AvaI CYCGRG 1 cut(s) 1200
AvaII GGWCC 3 cut(s) 413, 579, 989
BanII GRGCYC 2 cut(s) 424, 549
BbsI GAAGAC 3 cut(s) 1142, 1234, 1344
Bbv12I GWGCWC 3 cut(s) 424, 549, 693
BbvCI CCTCAGC 1 cut(s) 1206
BbvI GCAGC 3 cut(s) 451, 1019, 1072
BccI CCATC 4 cut(s) 311, 374, 614, 1022
BceAI ACGGC 1 cut(s) 713
BcgI CGANNNNNNTGC 2 cut(s) 845, 879
BciT130I CCWGG 1 cut(s) 1219
BclI TGATCA 1 cut(s) 352
BcoDI GTCTC 5 cut(s) 18, 147, 197, 411, 1277
BfaI CTAG 3 cut(s) 254, 410, 1121
BfmI CTRYAG 1 cut(s) 735
BisI GCNGC 3 cut(s) 465, 1033, 1086
BlpI GCTNAGC 2 cut(s) 390, 543
BlsI GCNGC 3 cut(s) 466, 1034, 1087
Bme1390I CCNGG 1 cut(s) 1219
Bme18I GGWCC 3 cut(s) 413, 579, 989
BmeT110I CYCGRG 1 cut(s) 1200
BmgBI CACGTC 1 cut(s) 1162
BmgT120I GGNCC 3 cut(s) 413, 579, 989
BmiI GGNNCC 2 cut(s) 581, 1225
BmrFI CCNGG 1 cut(s) 1219
BmrI ACTGGG 1 cut(s) 639
BmsI GCATC 2 cut(s) 608, 1268
BmuI ACTGGG 1 cut(s) 639
BpiI GAAGAC 3 cut(s) 1142, 1234, 1344
Bpu10I CCTNAGC 1 cut(s) 1206
Bpu1102I GCTNAGC 2 cut(s) 390, 543
Bpu14I TTCGAA 1 cut(s) 1093
BpuEI CTTGAG 4 cut(s) 79, 460, 677, 913
BsaAI YACGTR 2 cut(s) 1249, 1330
BsaBI GATNNNNATC 2 cut(s) 282, 795
BsaHI GRCGYC 1 cut(s) 456
BsaI GGTCTC 1 cut(s) 18
BsaJI CCNNGG 2 cut(s) 71, 1218
BsaWI WCCGGW 1 cut(s) 1398
BsaXI ACNNNNNCTCC 2 cut(s) 418, 448
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 1078, 1207
Bse118I RCCGGY 1 cut(s) 1145
Bse1I ACTGG 5 cut(s) 499, 634, 825, 1258, 1271
Bse3DI GCAATG 1 cut(s) 1072
Bse8I GATNNNNATC 2 cut(s) 282, 795
BseAI TCCGGA 1 cut(s) 1398
BseBI CCWGG 1 cut(s) 1219
BseDI CCNNGG 2 cut(s) 71, 1218
BseGI GGATG 1 cut(s) 340
BseJI GATNNNNATC 2 cut(s) 282, 795
BseLI CCNNNNNNNGG 3 cut(s) 73, 1078, 1207
BseMI GCAATG 1 cut(s) 1072
BseMII CTCAG 6 cut(s) 381, 534, 562, 642, 1098, 1197
BseNI ACTGG 5 cut(s) 499, 634, 825, 1258, 1271
BseRI GAGGAG 1 cut(s) 772
BseXI GCAGC 3 cut(s) 451, 1019, 1072
BsgI GTGCAG 3 cut(s) 828, 885, 1310
BshFI GGCC 1 cut(s) 637
BsiHKAI GWGCWC 3 cut(s) 424, 549, 693
BsiHKCI CYCGRG 1 cut(s) 1200
BsiSI CCGG 3 cut(s) 567, 1146, 1399
BslFI GGGAC 1 cut(s) 515
BslI CCNNNNNNNGG 3 cut(s) 73, 1078, 1207
BsmAI GTCTC 5 cut(s) 18, 147, 197, 411, 1277
BsmFI GGGAC 1 cut(s) 515
BsnI GGCC 1 cut(s) 637
Bso31I GGTCTC 1 cut(s) 18
BsoBI CYCGRG 1 cut(s) 1200
Bsp119I TTCGAA 1 cut(s) 1093
Bsp1286I GDGCHC 3 cut(s) 424, 549, 693
Bsp13I TCCGGA 1 cut(s) 1398
Bsp143I GATC 6 cut(s) 352, 784, 796, 818, 904, 1406
Bsp1720I GCTNAGC 2 cut(s) 390, 543
BspACI CCGC 1 cut(s) 726
BspANI GGCC 1 cut(s) 637
BspCNI CTCAG 6 cut(s) 382, 535, 561, 643, 1099, 1198
BspEI TCCGGA 1 cut(s) 1398
BspLI GGNNCC 2 cut(s) 581, 1225
BspPI GGATC 1 cut(s) 826
BspQI GCTCTTC 1 cut(s) 292
BspT104I TTCGAA 1 cut(s) 1093
BspTNI GGTCTC 1 cut(s) 18
BsrDI GCAATG 1 cut(s) 1072
BsrFI RCCGGY 1 cut(s) 1145
BsrI ACTGG 5 cut(s) 499, 634, 825, 1258, 1271
BssAI RCCGGY 1 cut(s) 1145
BssECI CCNNGG 2 cut(s) 71, 1218
BssMI GATC 6 cut(s) 352, 784, 796, 818, 904, 1406
BssNI GRCGYC 1 cut(s) 456
BssT1I CCWWGG 1 cut(s) 71
Bst2UI CCWGG 1 cut(s) 1219
Bst4CI ACNGT 7 cut(s) 82, 405, 806, 853, 883, 931, 976
Bst6I CTCTTC 1 cut(s) 292
BstACI GRCGYC 1 cut(s) 456
BstBAI YACGTR 2 cut(s) 1249, 1330
BstBI TTCGAA 1 cut(s) 1093
BstC8I GCNNGC 4 cut(s) 51, 150, 511, 515
BstDEI CTNAG 7 cut(s) 390, 543, 548, 651, 912, 1107, 1206
BstF5I GGATG 1 cut(s) 340
BstHHI GCGC 1 cut(s) 714
BstKTI GATC 6 cut(s) 355, 787, 799, 821, 907, 1409
BstMAI GTCTC 5 cut(s) 18, 147, 197, 411, 1277
BstMBI GATC 6 cut(s) 352, 784, 796, 818, 904, 1406
BstMWI GCNNNNNNNGC 3 cut(s) 55, 539, 725
BstNI CCWGG 1 cut(s) 1219
BstNSI RCATGY 1 cut(s) 1345
BstSCI CCNGG 1 cut(s) 1217
BstSFI CTRYAG 1 cut(s) 735
BstV1I GCAGC 3 cut(s) 451, 1019, 1072
BstV2I GAAGAC 3 cut(s) 1142, 1234, 1344
BsuRI GGCC 1 cut(s) 637
BtrI CACGTC 1 cut(s) 1162
BtsCI GGATG 1 cut(s) 340
BtsIMutI CAGTG 5 cut(s) 348, 456, 811, 832, 1278
Cac8I GCNNGC 4 cut(s) 51, 150, 511, 515
CaiI CAGNNNCTG 3 cut(s) 49, 271, 815
CfoI GCGC 1 cut(s) 714
Cfr10I RCCGGY 1 cut(s) 1145
Cfr13I GGNCC 3 cut(s) 413, 579, 989
CseI GACGC 2 cut(s) 445, 644
Csp6I GTAC 1 cut(s) 1345
CviAII CATG 3 cut(s) 344, 968, 1342
CviQI GTAC 1 cut(s) 1345
DdeI CTNAG 7 cut(s) 390, 543, 548, 651, 912, 1107, 1206
DpnI GATC 6 cut(s) 354, 786, 798, 820, 906, 1408
DpnII GATC 6 cut(s) 352, 784, 796, 818, 904, 1406
Eam1104I CTCTTC 1 cut(s) 292
EarI CTCTTC 1 cut(s) 292
Ecl136II GAGCTC 2 cut(s) 422, 547
Eco130I CCWWGG 1 cut(s) 71
Eco147I AGGCCT 1 cut(s) 637
Eco24I GRGCYC 2 cut(s) 424, 549
Eco31I GGTCTC 1 cut(s) 18
Eco47I GGWCC 3 cut(s) 413, 579, 989
Eco53kI GAGCTC 2 cut(s) 422, 547
Eco57I CTGAAG 3 cut(s) 297, 1005, 1068
Eco88I CYCGRG 1 cut(s) 1200
EcoICRI GAGCTC 2 cut(s) 422, 547
EcoRI GAATTC 1 cut(s) 396
EcoRII CCWGG 1 cut(s) 1217
EcoT14I CCWWGG 1 cut(s) 71
EcoT38I GRGCYC 2 cut(s) 424, 549
ErhI CCWWGG 1 cut(s) 71
FaeI CATG 3 cut(s) 347, 971, 1345
FalI AAGNNNNNCTT 6 cut(s) 286, 318, 905, 937, 1087, 1119
FaqI GGGAC 1 cut(s) 515
FatI CATG 3 cut(s) 343, 967, 1341
FbaI TGATCA 1 cut(s) 352
Fnu4HI GCNGC 3 cut(s) 465, 1033, 1086
FokI GGATG 1 cut(s) 347
FriOI GRGCYC 2 cut(s) 424, 549
Fsp4HI GCNGC 3 cut(s) 465, 1033, 1086
FspBI CTAG 3 cut(s) 254, 410, 1121
GlaI GCGC 1 cut(s) 713
GluI GCNGC 3 cut(s) 465, 1033, 1086
HaeIII GGCC 1 cut(s) 637
HapII CCGG 3 cut(s) 567, 1146, 1399
HgaI GACGC 2 cut(s) 445, 644
HhaI GCGC 1 cut(s) 714
Hin1I GRCGYC 1 cut(s) 456
Hin1II CATG 3 cut(s) 347, 971, 1345
Hin6I GCGC 1 cut(s) 712
HinP1I GCGC 1 cut(s) 712
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HinfI GANTC 5 cut(s) 283, 647, 812, 1165, 1377
HpaII CCGG 3 cut(s) 567, 1146, 1399
HphI GGTGA 5 cut(s) 133, 607, 758, 1145, 1189
Hpy166II GTNNAC 5 cut(s) 31, 64, 610, 889, 1237
Hpy188III TCNNGA 9 cut(s) 134, 591, 788, 816, 902, 1004, 1263, 1399, 1452
Hpy8I GTNNAC 5 cut(s) 31, 64, 610, 889, 1237
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 5 cut(s) 27, 492, 980, 1043, 1089
HpyCH4III ACNGT 7 cut(s) 82, 405, 806, 853, 883, 931, 976
HpyCH4IV ACGT 4 cut(s) 1161, 1239, 1248, 1329
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 539, 725
HpyF3I CTNAG 7 cut(s) 390, 543, 548, 651, 912, 1107, 1206
HpySE526I ACGT 4 cut(s) 1161, 1239, 1248, 1329
Hsp92I GRCGYC 1 cut(s) 456
Hsp92II CATG 3 cut(s) 347, 971, 1345
HspAI GCGC 1 cut(s) 712
Kpn2I TCCGGA 1 cut(s) 1398
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 6 cut(s) 352, 784, 796, 818, 904, 1406
LguI GCTCTTC 1 cut(s) 292
LmnI GCTCC 1 cut(s) 426
Lsp1109I GCAGC 3 cut(s) 451, 1019, 1072
LweI GCATC 2 cut(s) 608, 1268
MaeI CTAG 3 cut(s) 254, 410, 1121
MaeII ACGT 4 cut(s) 1161, 1239, 1248, 1329
MaeIII GTNAC 7 cut(s) 76, 346, 847, 1162, 1195, 1249, 1273
MalI GATC 6 cut(s) 354, 786, 798, 820, 906, 1408
MboI GATC 6 cut(s) 352, 784, 796, 818, 904, 1406
MboII GAAGA 6 cut(s) 303, 309, 1142, 1234, 1346, 1349
MfeI CAATTG 1 cut(s) 1287
MhlI GDGCHC 3 cut(s) 424, 549, 693
MlyI GAGTC 1 cut(s) 1159
MroI TCCGGA 1 cut(s) 1398
MroXI GAANNNNTTC 1 cut(s) 1241
MseI TTAA 4 cut(s) 117, 669, 684, 941
MslI CAYNNNNRTG 1 cut(s) 1026
MspI CCGG 3 cut(s) 567, 1146, 1399
MspR9I CCNGG 1 cut(s) 1219
MunI CAATTG 1 cut(s) 1287
MvaI CCWGG 1 cut(s) 1219
MwoI GCNNNNNNNGC 3 cut(s) 55, 539, 725
NdeII GATC 6 cut(s) 352, 784, 796, 818, 904, 1406
NlaIII CATG 3 cut(s) 347, 971, 1345
NlaIV GGNNCC 2 cut(s) 581, 1225
NmuCI GTSAC 5 cut(s) 346, 1162, 1195, 1249, 1273
NspI RCATGY 1 cut(s) 1345
NspV TTCGAA 1 cut(s) 1093
PceI AGGCCT 1 cut(s) 637
PciI ACATGT 1 cut(s) 1341
PciSI GCTCTTC 1 cut(s) 292
PcsI WCGNNNNNNNCGW 2 cut(s) 430, 1245
PdmI GAANNNNTTC 1 cut(s) 1241
PfeI GAWTC 4 cut(s) 283, 647, 812, 1377
PflMI CCANNNNNTGG 1 cut(s) 1078
PkrI GCNGC 3 cut(s) 466, 1034, 1087
PleI GAGTC 1 cut(s) 1159
PpsI GAGTC 1 cut(s) 1159
Ppu21I YACGTR 2 cut(s) 1249, 1330
PscI ACATGT 1 cut(s) 1341
PsiI TTATAA 1 cut(s) 293
Psp124BI GAGCTC 2 cut(s) 424, 549
Psp6I CCWGG 1 cut(s) 1217
PspGI CCWGG 1 cut(s) 1217
PspN4I GGNNCC 2 cut(s) 581, 1225
PspPI GGNCC 3 cut(s) 413, 579, 989
PsrI GAACNNNNNNTAC 2 cut(s) 1229, 1261
PstNI CAGNNNCTG 3 cut(s) 49, 271, 815
RsaI GTAC 1 cut(s) 1346
RsaNI GTAC 1 cut(s) 1345
RseI CAYNNNNRTG 1 cut(s) 1026
SacI GAGCTC 2 cut(s) 424, 549
SapI GCTCTTC 1 cut(s) 292
SaqAI TTAA 4 cut(s) 117, 669, 684, 941
SatI GCNGC 3 cut(s) 465, 1033, 1086
Sau3AI GATC 6 cut(s) 352, 784, 796, 818, 904, 1406
Sau96I GGNCC 3 cut(s) 413, 579, 989
SchI GAGTC 1 cut(s) 1159
ScrFI CCNGG 1 cut(s) 1219
SduI GDGCHC 3 cut(s) 424, 549, 693
SfaNI GCATC 2 cut(s) 608, 1268
SfcI CTRYAG 1 cut(s) 735
SfuI TTCGAA 1 cut(s) 1093
SinI GGWCC 3 cut(s) 413, 579, 989
SmiMI CAYNNNNRTG 1 cut(s) 1026
SmlI CTYRAG 4 cut(s) 94, 439, 692, 892
SmoI CTYRAG 4 cut(s) 94, 439, 692, 892
SseBI AGGCCT 1 cut(s) 637
SsiI CCGC 1 cut(s) 726
SspI AATATT 1 cut(s) 1010
SspMI CTAG 3 cut(s) 254, 410, 1121
SstI GAGCTC 2 cut(s) 424, 549
StuI AGGCCT 1 cut(s) 637
StyD4I CCNGG 1 cut(s) 1217
StyI CCWWGG 1 cut(s) 71
TaaI ACNGT 7 cut(s) 82, 405, 806, 853, 883, 931, 976
TaiI ACGT 4 cut(s) 1164, 1242, 1251, 1332
TaqI TCGA 2 cut(s) 855, 1093
TatI WGTACW 1 cut(s) 1344
TfiI GAWTC 4 cut(s) 283, 647, 812, 1377
Tru1I TTAA 4 cut(s) 117, 669, 684, 941
Tru9I TTAA 4 cut(s) 117, 669, 684, 941
TscAI CASTG 5 cut(s) 355, 456, 811, 832, 1278
TseFI GTSAC 5 cut(s) 346, 1162, 1195, 1249, 1273
TseI GCWGC 3 cut(s) 464, 1032, 1085
Tsp45I GTSAC 5 cut(s) 346, 1162, 1195, 1249, 1273
TspDTI ATGAA 1 cut(s) 332
TspGWI ACGGA 1 cut(s) 422
TspRI CASTG 5 cut(s) 355, 456, 811, 832, 1278
Van91I CCANNNNNTGG 1 cut(s) 1078
VpaK11BI GGWCC 3 cut(s) 413, 579, 989
XapI RAATTY 4 cut(s) 128, 212, 396, 1415
XceI RCATGY 1 cut(s) 1345
XmnI GAANNNNTTC 1 cut(s) 1241
XspI CTAG 3 cut(s) 254, 410, 1121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.