FvH4_4g23470

Sphingosine-1-phosphate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011402
Physical Location & Seq
Reverse (-)
25925942 .. 25931692
5751 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23470.t5

Sequence Viewer

Length: 1626 bp
ATGGATTTCTCTCCAGTCAAATCGTTCCTGCTCGGAGCTCGGGCTTCGGCGAATTCCTTCTTGGAAGGCTACGAGCCGCTGGTTCTGTTTTCGACGCCGCTGATCGGACTCTTGGTCGCGTCAAGGCTTCAGTCGGTGATCAGTGTCCTGCAGGAGAACGGAGTCAAGGCCACTCTCATAGGCTTGTTCATGAGCTTCATCAAGCTGGTTCCTGGAGTGAAGAAGTACATAGACGCCGAGAAGCAAAAGGTGGTGGATCAGTTACAGTCTGGTGGTAACTCTAAAAGAGAGAATTGGAGAACTGAGTTGCCTGGCGCGGGGTTGGGAGCGGGAGTTCTTGACCAGATGAAAGAGGAGAAGAGAAAAGATGTGGCTTGGCAAGGCAAATGCTCTGGTACAGTATATATTGGAGGAAGTGAATTCGAAGGACATTTTTCTCTAATTAATGAGGCATGCTCAATGTTTGCACATACCAATCCACTACATATGGATGTATTCCAGAGTGTTGTACGGTTTGAAGCAGAAGTGGTTGCGATGACAGCTGCAATGGTTGGAAGTAAAACAAAGTCTTCCGGAGGACAAATATGTGGAAACATGACATCAGGAGGAACTGAAAGTATATTACTAGCTGTGAAAACATCACGTGATTATATGAAAGCCAGGAAGGGGATTAAAAAACCTGAAATGATAATACCTGAATCTGCACACTCAGCATACGACAAGGCTGCACAATATTTTAACATCAAGCTCTGGCGTGTCCCTGTAGATAAAGAGTTCCAAGCAGATGTCAAAGCGATCAGAAAATTCATTAATAGAAACACCGTTTTGATTGTTGGATCTGCACCTGGTTTTCCTCATGGCATTATTGATCCTATTGAGGAACTTGGTCAATTAGCTTCTAGCTTTGATATCTGTCTCCATGTCGACCTTTGTCTTGGGGGATTTGTACTACCTTTCGCTCAAAAGCTGGGGTATCCGATTCCACCCTTTGATTTTTCAGTTAAAGGAGTGACATCAATATCAGCTGATGTGCATAAATATGGGTTGGCTCCTAAAGGAACAAGCATAGTTCTGTACAGAAACCATGACATTAGAAAGCATCAATTTGTGGCTGTTACGGAGTGGTCAGGAGGGCTGTATGTATCTCCAACCATGGCTGGAAGCAGACCTGGTGGGTTAATTGCTGGGGCTTGGGCAGCTATGATGGCTTTGGGGGAGGAAGGGTATCTGGAAAACACAAGGGCTATCATGGAAGTATCCAAAAAACTTCAAAAAGGGTTAGAGGATATTCCCGAGTTGTTCATCATTGGACGGCCAGACATGACAGTTGTTGCATTTGGTTCTAATGTTGTTGATATATTTGAAGTCAATGATATCATGTCATCTAAAGGCTGGCATTTGAATGCATTGCAAAGACCTAACAGCATTCACATATGTATGACACTTCAACATGTACCAATTCTTGATGACTTTCTGAGGGATCTAAGGGAATCTGTGAAAACCGTAAAAGAAAATCCAGGTCCCATAAGTGGAGGGCTCGCTCCTATTTATGGTGCTGCAGGGAGGATGCCAGATAGAGGTATGGTCCAGGATCTGCTGGTAAATTACATGGACGGTACATGCTAG

Protein Analysis

542

Amino Acids

58.91

Weight (kDa)

7.62

Isoelectric Point (pI)

36.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyridoxal_deC PF00282 146 - 426 4.8e-19 Pyridoxal-dependent decarboxylase conserved domain
Aminotran_5 PF00266 200 - 362 3e-08 Aminotransferase class-V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011402)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27980
fragaria_vesca FvH4_4g23470 FvH4_4g23470 FvH4_4g23470 FvH4_4g23470 FvH4_4g23470 FvH4_4g23470 FvH4_4g23470
malus_domestica MD13G1122300.v1.1
prunus_persica Prupe.1G222900_v2.0.a1
pyrus_communis pycom13g10640
rosa_chinensis RchiOBHm_Chr4g0429841
rosa_laevigata RLG00000007039
rosa_multiflora Rmu_sc0006762.1_g000006
rosa_roxburghii Rroxscaffold_5G00371600
rosa_rugosa Rorug04G0238500
rosa_samantha Rh4AG294900 Rh4BG301300 Rh4DG298100
rosa_wichuraiana Rw4G025510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 329
AccI GTMKAC 1 cut(s) 924
AccII CGCG 2 cut(s) 119, 317
AccIII TCCGGA 1 cut(s) 572
AciI CCGC 4 cut(s) 77, 98, 317, 329
AclWI GGATC 5 cut(s) 264, 844, 863, 1488, 1599
AcoI YGGCCR 1 cut(s) 1313
AcsI RAATTY 3 cut(s) 52, 419, 803
AcuI CTGAAG 1 cut(s) 113
AcvI CACGTG 1 cut(s) 644
AcyI GRCGYC 2 cut(s) 95, 234
AfaI GTAC 7 cut(s) 227, 397, 510, 948, 1076, 1455, 1618
AfiI CCNNNNNNNGG 6 cut(s) 104, 317, 666, 1529, 1550, 1577
AflIII ACRYGT 1 cut(s) 1450
AgsI TTSAA 5 cut(s) 518, 1271, 1364, 1402, 1448
AhdI GACNNNNNGTC 1 cut(s) 113
AjnI CCWGG 7 cut(s) 211, 310, 659, 844, 1168, 1516, 1587
AjuI GAANNNNNNNTTGG 2 cut(s) 44, 76
AloI GAACNNNNNNTCC 2 cut(s) 318, 350
Alw21I GWGCWC 1 cut(s) 40
Alw26I GTCTC 1 cut(s) 920
AlwI GGATC 5 cut(s) 264, 844, 863, 1488, 1599
AlwNI CAGNNNCTG 1 cut(s) 1594
Ama87I CYCGRG 2 cut(s) 39, 1292
Aor13HI TCCGGA 1 cut(s) 572
AoxI GGCC 2 cut(s) 168, 1313
ApeKI GCWGC 4 cut(s) 542, 725, 1196, 1556
ApoI RAATTY 3 cut(s) 52, 419, 803
AseI ATTAAT 2 cut(s) 444, 810
Asp700I GAANNNNTTC 1 cut(s) 56
AspLEI GCGC 1 cut(s) 317
AspS9I GGNCC 2 cut(s) 1520, 1585
AsuHPI GGTGA 1 cut(s) 148
AsuII TTCGAA 1 cut(s) 423
AvaI CYCGRG 2 cut(s) 39, 1292
AvaII GGWCC 2 cut(s) 1520, 1585
BanII GRGCYC 2 cut(s) 40, 1539
BbrPI CACGTG 1 cut(s) 644
BbsI GAAGAC 1 cut(s) 561
Bbv12I GWGCWC 1 cut(s) 40
BbvI GCAGC 4 cut(s) 529, 712, 1208, 1543
BccI CCATC 1 cut(s) 1198
BceAI ACGGC 1 cut(s) 1328
BcgI CGANNNNNNTGC 2 cut(s) 707, 741
BciT130I CCWGG 7 cut(s) 213, 312, 661, 846, 1170, 1518, 1589
BciVI GTATCC 2 cut(s) 984, 1267
BclI TGATCA 1 cut(s) 138
BcoDI GTCTC 1 cut(s) 920
BfaI CTAG 3 cut(s) 626, 900, 1624
BfmI CTRYAG 3 cut(s) 149, 762, 1557
BfuI GTATCC 2 cut(s) 984, 1267
BisI GCNGC 6 cut(s) 77, 98, 543, 726, 1197, 1557
BlsI GCNGC 6 cut(s) 78, 99, 544, 727, 1198, 1558
Bme1390I CCNGG 7 cut(s) 213, 312, 661, 846, 1170, 1518, 1589
Bme18I GGWCC 2 cut(s) 1520, 1585
BmeRI GACNNNNNGTC 1 cut(s) 113
BmeT110I CYCGRG 2 cut(s) 39, 1292
BmgT120I GGNCC 2 cut(s) 1520, 1585
BmiI GGNNCC 3 cut(s) 210, 1050, 1522
BmrFI CCNGG 7 cut(s) 213, 312, 661, 846, 1170, 1518, 1589
BmsI GCATC 2 cut(s) 1108, 1557
BoxI GACNNNNGTC 1 cut(s) 930
BpiI GAAGAC 1 cut(s) 561
BplI GAGNNNNNCTC 2 cut(s) 440, 472
BpmI CTGGAG 1 cut(s) 234
Bpu14I TTCGAA 1 cut(s) 423
BsaAI YACGTR 1 cut(s) 644
BsaHI GRCGYC 2 cut(s) 95, 234
BsaJI CCNNGG 1 cut(s) 1152
BsaWI WCCGGW 1 cut(s) 572
BsaXI ACNNNNNCTCC 4 cut(s) 318, 348, 1208, 1238
Bsc4I CCNNNNNNNGG 6 cut(s) 104, 317, 666, 1529, 1550, 1577
Bse1I ACTGG 1 cut(s) 14
Bse3DI GCAATG 2 cut(s) 552, 1406
BseAI TCCGGA 1 cut(s) 572
BseBI CCWGG 7 cut(s) 213, 312, 661, 846, 1170, 1518, 1589
BseDI CCNNGG 1 cut(s) 1152
BseGI GGATG 2 cut(s) 496, 1572
BseLI CCNNNNNNNGG 6 cut(s) 104, 317, 666, 1529, 1550, 1577
BseMI GCAATG 2 cut(s) 552, 1406
BseMII CTCAG 3 cut(s) 294, 723, 1466
BseNI ACTGG 1 cut(s) 14
BseRI GAGGAG 1 cut(s) 368
BseXI GCAGC 4 cut(s) 529, 712, 1208, 1543
BseYI CCCAGC 2 cut(s) 967, 1184
BsgI GTGCAG 3 cut(s) 687, 711, 825
Bsh1236I CGCG 2 cut(s) 119, 317
BshFI GGCC 2 cut(s) 170, 1315
BsiHKAI GWGCWC 1 cut(s) 40
BsiHKCI CYCGRG 2 cut(s) 39, 1292
BsiSI CCGG 1 cut(s) 573
BslFI GGGAC 2 cut(s) 743, 1506
BslI CCNNNNNNNGG 6 cut(s) 104, 317, 666, 1529, 1550, 1577
BsmAI GTCTC 1 cut(s) 920
BsmFI GGGAC 2 cut(s) 743, 1506
BsmI GAATGC 2 cut(s) 1408, 1425
BsnI GGCC 2 cut(s) 170, 1315
BsoBI CYCGRG 2 cut(s) 39, 1292
Bsp119I TTCGAA 1 cut(s) 423
Bsp1286I GDGCHC 2 cut(s) 40, 1539
Bsp13I TCCGGA 1 cut(s) 572
Bsp1407I TGTACA 1 cut(s) 1074
Bsp143I GATC 8 cut(s) 102, 138, 256, 795, 836, 868, 1480, 1591
Bsp19I CCATGG 1 cut(s) 1152
BspACI CCGC 4 cut(s) 77, 98, 317, 329
BspANI GGCC 2 cut(s) 170, 1315
BspCNI CTCAG 3 cut(s) 295, 722, 1467
BspEI TCCGGA 1 cut(s) 572
BspFNI CGCG 2 cut(s) 119, 317
BspHI TCATGA 1 cut(s) 189
BspLI GGNNCC 3 cut(s) 210, 1050, 1522
BspMAI CTGCAG 2 cut(s) 153, 1561
BspPI GGATC 5 cut(s) 264, 844, 863, 1488, 1599
BspT104I TTCGAA 1 cut(s) 423
BsrBI CCGCTC 1 cut(s) 329
BsrDI GCAATG 2 cut(s) 552, 1406
BsrGI TGTACA 1 cut(s) 1074
BsrI ACTGG 1 cut(s) 14
BssECI CCNNGG 1 cut(s) 1152
BssMI GATC 8 cut(s) 102, 138, 256, 795, 836, 868, 1480, 1591
BssNI GRCGYC 2 cut(s) 95, 234
BssT1I CCWWGG 1 cut(s) 1152
Bst2UI CCWGG 7 cut(s) 213, 312, 661, 846, 1170, 1518, 1589
Bst4CI ACNGT 7 cut(s) 267, 400, 513, 823, 1327, 1504, 1616
Bst6I CTCTTC 1 cut(s) 353
BstACI GRCGYC 2 cut(s) 95, 234
BstAUI TGTACA 1 cut(s) 1074
BstBAI YACGTR 1 cut(s) 644
BstBI TTCGAA 1 cut(s) 423
BstC8I GCNNGC 3 cut(s) 454, 1394, 1539
BstDEI CTNAG 4 cut(s) 303, 709, 1475, 1484
BstDSI CCRYGG 1 cut(s) 1152
BstF5I GGATG 2 cut(s) 496, 1572
BstFNI CGCG 2 cut(s) 119, 317
BstHHI GCGC 1 cut(s) 317
BstKTI GATC 8 cut(s) 105, 141, 259, 798, 839, 871, 1483, 1594
BstMAI GTCTC 1 cut(s) 920
BstMBI GATC 8 cut(s) 102, 138, 256, 795, 836, 868, 1480, 1591
BstMWI GCNNNNNNNGC 4 cut(s) 539, 710, 1196, 1205
BstNI CCWGG 7 cut(s) 213, 312, 661, 846, 1170, 1518, 1589
BstNSI RCATGY 3 cut(s) 456, 1454, 1623
BstPAI GACNNNNGTC 1 cut(s) 930
BstSCI CCNGG 7 cut(s) 211, 310, 659, 844, 1168, 1516, 1587
BstSFI CTRYAG 3 cut(s) 149, 762, 1557
BstUI CGCG 2 cut(s) 119, 317
BstV1I GCAGC 4 cut(s) 529, 712, 1208, 1543
BstV2I GAAGAC 1 cut(s) 561
BstX2I RGATCY 3 cut(s) 836, 1480, 1591
BstYI RGATCY 3 cut(s) 836, 1480, 1591
BsuI GTATCC 2 cut(s) 984, 1267
BsuRI GGCC 2 cut(s) 170, 1315
BtgI CCRYGG 1 cut(s) 1152
BtgZI GCGATG 1 cut(s) 548
BtsCI GGATG 2 cut(s) 496, 1572
BtsIMutI CAGTG 1 cut(s) 148
Cac8I GCNNGC 3 cut(s) 454, 1394, 1539
CaiI CAGNNNCTG 1 cut(s) 1594
CciI TCATGA 1 cut(s) 189
CfoI GCGC 1 cut(s) 317
Cfr13I GGNCC 2 cut(s) 1520, 1585
CseI GACGC 3 cut(s) 103, 108, 242
CsiI ACCWGGT 2 cut(s) 844, 1168
Csp6I GTAC 7 cut(s) 226, 396, 509, 947, 1075, 1454, 1617
CviQI GTAC 7 cut(s) 226, 396, 509, 947, 1075, 1454, 1617
DdeI CTNAG 4 cut(s) 303, 709, 1475, 1484
DpnI GATC 8 cut(s) 104, 140, 258, 797, 838, 870, 1482, 1593
DpnII GATC 8 cut(s) 102, 138, 256, 795, 836, 868, 1480, 1591
DriI GACNNNNNGTC 1 cut(s) 113
EaeI YGGCCR 1 cut(s) 1313
Eam1104I CTCTTC 1 cut(s) 353
Eam1105I GACNNNNNGTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 353
Ecl136II GAGCTC 1 cut(s) 38
Eco130I CCWWGG 1 cut(s) 1152
Eco24I GRGCYC 2 cut(s) 40, 1539
Eco32I GATATC 2 cut(s) 910, 1375
Eco47I GGWCC 2 cut(s) 1520, 1585
Eco53kI GAGCTC 1 cut(s) 38
Eco57I CTGAAG 1 cut(s) 113
Eco72I CACGTG 1 cut(s) 644
Eco88I CYCGRG 2 cut(s) 39, 1292
EcoICRI GAGCTC 1 cut(s) 38
EcoO109I RGGNCCY 1 cut(s) 1520
EcoRI GAATTC 2 cut(s) 52, 419
EcoRII CCWGG 7 cut(s) 211, 310, 659, 844, 1168, 1516, 1587
EcoRV GATATC 2 cut(s) 910, 1375
EcoT14I CCWWGG 1 cut(s) 1152
EcoT22I ATGCAT 1 cut(s) 1408
EcoT38I GRGCYC 2 cut(s) 40, 1539
ErhI CCWWGG 1 cut(s) 1152
FaqI GGGAC 2 cut(s) 743, 1506
FauI CCCGC 2 cut(s) 310, 322
FauNDI CATATG 2 cut(s) 486, 1433
FbaI TGATCA 1 cut(s) 138
FblI GTMKAC 1 cut(s) 924
Fnu4HI GCNGC 6 cut(s) 77, 98, 543, 726, 1197, 1557
FokI GGATG 2 cut(s) 503, 1579
FriOI GRGCYC 2 cut(s) 40, 1539
Fsp4HI GCNGC 6 cut(s) 77, 98, 543, 726, 1197, 1557
FspBI CTAG 3 cut(s) 626, 900, 1624
GlaI GCGC 1 cut(s) 316
GluI GCNGC 6 cut(s) 77, 98, 543, 726, 1197, 1557
GsaI CCCAGC 2 cut(s) 971, 1188
GsuI CTGGAG 1 cut(s) 234
HaeIII GGCC 2 cut(s) 170, 1315
HapII CCGG 1 cut(s) 573
HgaI GACGC 3 cut(s) 103, 108, 242
HhaI GCGC 1 cut(s) 317
Hin1I GRCGYC 2 cut(s) 95, 234
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HincII GTYRAC 1 cut(s) 925
HindII GTYRAC 1 cut(s) 925
HinfI GANTC 5 cut(s) 108, 162, 698, 979, 1490
HpaII CCGG 1 cut(s) 573
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 1 cut(s) 925
Hpy188I TCNGA 5 cut(s) 35, 107, 800, 978, 1476
Hpy188III TCNNGA 9 cut(s) 190, 338, 499, 573, 603, 1128, 1229, 1292, 1463
Hpy8I GTNNAC 1 cut(s) 925
Hpy99I CGWCG 1 cut(s) 97
HpyAV CCTTC 5 cut(s) 59, 67, 419, 658, 1214
HpyCH4III ACNGT 7 cut(s) 267, 400, 513, 823, 1327, 1504, 1616
HpyCH4IV ACGT 1 cut(s) 643
HpyF10VI GCNNNNNNNGC 4 cut(s) 539, 710, 1196, 1205
HpyF3I CTNAG 4 cut(s) 303, 709, 1475, 1484
HpySE526I ACGT 1 cut(s) 643
Hsp92I GRCGYC 2 cut(s) 95, 234
HspAI GCGC 1 cut(s) 315
Kpn2I TCCGGA 1 cut(s) 572
Ksp22I TGATCA 1 cut(s) 138
Kzo9I GATC 8 cut(s) 102, 138, 256, 795, 836, 868, 1480, 1591
LmnI GCTCC 4 cut(s) 35, 326, 1054, 1546
Lsp1109I GCAGC 4 cut(s) 529, 712, 1208, 1543
LweI GCATC 2 cut(s) 1108, 1557
MabI ACCWGGT 2 cut(s) 844, 1168
MaeI CTAG 3 cut(s) 626, 900, 1624
MaeII ACGT 1 cut(s) 643
MaeIII GTNAC 4 cut(s) 261, 275, 1009, 1114
MalI GATC 8 cut(s) 104, 140, 258, 797, 838, 870, 1482, 1593
MbiI CCGCTC 1 cut(s) 329
MboI GATC 8 cut(s) 102, 138, 256, 795, 836, 868, 1480, 1591
MboII GAAGA 3 cut(s) 232, 370, 561
MflI RGATCY 3 cut(s) 836, 1480, 1591
MhlI GDGCHC 2 cut(s) 40, 1539
MlyI GAGTC 2 cut(s) 102, 171
MmeI TCCRAC 3 cut(s) 532, 814, 1172
Mph1103I ATGCAT 1 cut(s) 1408
MroI TCCGGA 1 cut(s) 572
MroXI GAANNNNTTC 1 cut(s) 56
MseI TTAA 6 cut(s) 444, 672, 738, 810, 1002, 1178
MslI CAYNNNNRTG 4 cut(s) 489, 1038, 1401, 1436
MspA1I CMGCKG 4 cut(s) 79, 100, 542, 1025
MspI CCGG 1 cut(s) 573
MspR9I CCNGG 7 cut(s) 213, 312, 661, 846, 1170, 1518, 1589
Mva1269I GAATGC 2 cut(s) 1408, 1425
MvaI CCWGG 7 cut(s) 213, 312, 661, 846, 1170, 1518, 1589
MvnI CGCG 2 cut(s) 119, 317
MwoI GCNNNNNNNGC 4 cut(s) 539, 710, 1196, 1205
NcoI CCATGG 1 cut(s) 1152
NdeI CATATG 2 cut(s) 486, 1433
NdeII GATC 8 cut(s) 102, 138, 256, 795, 836, 868, 1480, 1591
NlaIV GGNNCC 3 cut(s) 210, 1050, 1522
NmeAIII GCCGAG 1 cut(s) 262
NmuCI GTSAC 1 cut(s) 1009
NsiI ATGCAT 1 cut(s) 1408
NspI RCATGY 3 cut(s) 456, 1454, 1623
NspV TTCGAA 1 cut(s) 423
PaeI GCATGC 1 cut(s) 456
PagI TCATGA 1 cut(s) 189
PciI ACATGT 1 cut(s) 1450
PctI GAATGC 2 cut(s) 1408, 1425
PdmI GAANNNNTTC 1 cut(s) 56
PfeI GAWTC 3 cut(s) 698, 979, 1490
PfoI TCCNGGA 2 cut(s) 211, 1587
PkrI GCNGC 6 cut(s) 78, 99, 544, 727, 1198, 1558
PleI GAGTC 2 cut(s) 102, 170
PmaCI CACGTG 1 cut(s) 644
PmlI CACGTG 1 cut(s) 644
PpsI GAGTC 2 cut(s) 102, 170
Ppu21I YACGTR 1 cut(s) 644
PpuMI RGGWCCY 1 cut(s) 1520
PscI ACATGT 1 cut(s) 1450
PshAI GACNNNNGTC 1 cut(s) 930
PshBI ATTAAT 2 cut(s) 444, 810
Psp124BI GAGCTC 1 cut(s) 40
Psp5II RGGWCCY 1 cut(s) 1520
Psp6I CCWGG 7 cut(s) 211, 310, 659, 844, 1168, 1516, 1587
PspCI CACGTG 1 cut(s) 644
PspFI CCCAGC 2 cut(s) 967, 1184
PspGI CCWGG 7 cut(s) 211, 310, 659, 844, 1168, 1516, 1587
PspN4I GGNNCC 3 cut(s) 210, 1050, 1522
PspPI GGNCC 2 cut(s) 1520, 1585
PspPPI RGGWCCY 1 cut(s) 1520
PstI CTGCAG 2 cut(s) 153, 1561
PstNI CAGNNNCTG 1 cut(s) 1594
PsuI RGATCY 3 cut(s) 836, 1480, 1591
PvuII CAGCTG 2 cut(s) 542, 1025
RsaI GTAC 7 cut(s) 227, 397, 510, 948, 1076, 1455, 1618
RsaNI GTAC 7 cut(s) 226, 396, 509, 947, 1075, 1454, 1617
RseI CAYNNNNRTG 4 cut(s) 489, 1038, 1401, 1436
SacI GAGCTC 1 cut(s) 40
SalI GTCGAC 1 cut(s) 923
SaqAI TTAA 6 cut(s) 444, 672, 738, 810, 1002, 1178
SatI GCNGC 6 cut(s) 77, 98, 543, 726, 1197, 1557
Sau3AI GATC 8 cut(s) 102, 138, 256, 795, 836, 868, 1480, 1591
Sau96I GGNCC 2 cut(s) 1520, 1585
SbfI CCTGCAGG 1 cut(s) 153
SchI GAGTC 2 cut(s) 102, 171
ScrFI CCNGG 7 cut(s) 213, 312, 661, 846, 1170, 1518, 1589
SdaI CCTGCAGG 1 cut(s) 153
SduI GDGCHC 2 cut(s) 40, 1539
SexAI ACCWGGT 2 cut(s) 844, 1168
SfaNI GCATC 2 cut(s) 1108, 1557
SfcI CTRYAG 3 cut(s) 149, 762, 1557
SfuI TTCGAA 1 cut(s) 423
SinI GGWCC 2 cut(s) 1520, 1585
SmiMI CAYNNNNRTG 4 cut(s) 489, 1038, 1401, 1436
SphI GCATGC 1 cut(s) 456
Sse8387I CCTGCAGG 1 cut(s) 153
SsiI CCGC 4 cut(s) 77, 98, 317, 329
SspI AATATT 1 cut(s) 734
SspMI CTAG 3 cut(s) 626, 900, 1624
SstI GAGCTC 1 cut(s) 40
StyD4I CCNGG 7 cut(s) 211, 310, 659, 844, 1168, 1516, 1587
StyI CCWWGG 1 cut(s) 1152
TaaI ACNGT 7 cut(s) 267, 400, 513, 823, 1327, 1504, 1616
TaiI ACGT 1 cut(s) 646
TaqI TCGA 3 cut(s) 92, 423, 924
TatI WGTACW 3 cut(s) 225, 946, 1074
TauI GCSGC 2 cut(s) 79, 100
TfiI GAWTC 3 cut(s) 698, 979, 1490
Tru1I TTAA 6 cut(s) 444, 672, 738, 810, 1002, 1178
Tru9I TTAA 6 cut(s) 444, 672, 738, 810, 1002, 1178
TscAI CASTG 1 cut(s) 148
TseFI GTSAC 1 cut(s) 1009
TseI GCWGC 4 cut(s) 542, 725, 1196, 1556
Tsp45I GTSAC 1 cut(s) 1009
TspDTI ATGAA 6 cut(s) 178, 187, 362, 668, 796, 1291
TspGWI ACGGA 2 cut(s) 174, 1133
TspRI CASTG 1 cut(s) 148
VpaK11BI GGWCC 2 cut(s) 1520, 1585
VspI ATTAAT 2 cut(s) 444, 810
XapI RAATTY 3 cut(s) 52, 419, 803
XceI RCATGY 3 cut(s) 456, 1454, 1623
XmiI GTMKAC 1 cut(s) 924
XmnI GAANNNNTTC 1 cut(s) 56
XspI CTAG 3 cut(s) 626, 900, 1624
Zsp2I ATGCAT 1 cut(s) 1408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.