Rroxscaffold_5G00371600

Sphingosine-1-phosphate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0011402
Physical Location & Seq
Reverse (-)
53174453 .. 53179650
5198 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00371600.1

Sequence Viewer

Length: 1443 bp
ATGGATTCTTCGTCGGTGAAATCGTTCCTGCTCGGCGCTCGAGCTTCGGCGAATTCGTTTTTGGCCGGTTACGAGCCTCTGGTTCTGGTTTCGACGCCTCTGATTGGACTCTTCGTTGCGTCGACGCTTCAATCGGTGCTTAGTGTTGTGCAGGAGAAGGGAGTCAACGCCACTCTGTTAGGGCTGTTCATGAGCTTCATCAAGCTGGTTCCTGGGGTGAAGAAGTACATAGACGCGGAGAAGCAAAAGGTGGTGGATAAATTACAGTCTGGTGGTAACTCTAAAAGAGAAAATTGGAGAACTGAATTGCCTGGCGCAGGGTTGGGAGCGGGAGTTCTGGAACAAATGAAAGACGAGAAAAGAAAAGATGTGGCTTGGCAAGGCAAATGCTCGGGTACAGTATATATTGGAGGAAGTGAATTCGAAGGACATTTTTCTTTAATTAATGAGGCATGCTCAATGTTTGCACATACCAATCCATTACATATGGATGTATTCCAGAGTGTTGTACAGTTTGAAGCAAGAGAGTGGATAATACCTGAATCTGCTCACTCAGCATACGACAAGGCTGCACAATATTTTAACATCAAGCTCTGGCGTGTCCCTGTAGATAAAGAGTTCCAAGCAGATGTCAAAGCCATTAGAAGATTCATTAATAGAAACACTGTTTTGATTGTTGGATCTGCACCTGGTTTTCCTCATGGCATTGTTGATCCTATTGAGGAGCTTGGTCAATTAGCTTCTAGCTTTGATATCTGTCTCCATGTCGACCTTTGTCTTGGGGGATTTGTACTACCTTTTGCTCAAAAGCTTGGGTATCCGATTCCACCCTTTGATTTTTCAGTTAAAGGAGTGACGTCAATATCAGCTGATGTGCATAAATATGGGTTGGCTCCTAAAGGAACAAGCATAGTTCTGTACAGAAACCATGGCATTAGAAAGCATCAATTTGTGGCTGTTACGGAGTGGTCAGGAGGGCTGTATGTATCTCCAACAATGGCTGGAAGCAGACCTGGTAGCTTAATTGCAGGGGCTTGGGCAGCTATGATGGCTTTGGGTGAGGAAGGGTATTTGGAAAACACAAGGTCTATCATGGAAGTATCCAAAAAACTTCAGAAAGGGATAAAGGATATTCCCGAGTTGTTCATCATTGGACGGCCAGACATGACAGTTGTTGCATTTGGCTCTAGCGTTGTTGATATATTTGAAGTCAATGATATCATGTCATCTAAAGGCTGGCATTTGAATGCATTGCAAAGACCTAACAGCATTCATATATGTATGACACTTCAACATGTACCAATTCTTGATGACTTTCTGAGGGATCTAAGGGAATCTGTGAAAACTGTCAAAGAAAATCCCGGACCCATAAGCGGAGGGCTTGCTCCCATTTACGGTGCTGCAGGGAGGATGCCAGATAGAGATTCATTGGTTAGGTTATAA

Protein Analysis

480

Amino Acids

52.44

Weight (kDa)

7.68

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyridoxal_deC PF00282 181 - 394 9e-18 Pyridoxal-dependent decarboxylase conserved domain
Aminotran_5 PF00266 183 - 409 9.4e-07 Aminotransferase class-V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011402)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27980
fragaria_vesca FvH4_4g23470 FvH4_4g23470 FvH4_4g23470 FvH4_4g23470 FvH4_4g23470 FvH4_4g23470 FvH4_4g23470
malus_domestica MD13G1122300.v1.1
prunus_persica Prupe.1G222900_v2.0.a1
pyrus_communis pycom13g10640
rosa_chinensis RchiOBHm_Chr4g0429841
rosa_laevigata RLG00000007039
rosa_multiflora Rmu_sc0006762.1_g000006
rosa_roxburghii Rroxscaffold_5G00371600
rosa_rugosa Rorug04G0238500
rosa_samantha Rh4AG294900 Rh4BG301300 Rh4DG298100
rosa_wichuraiana Rw4G025510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1441
AatII GACGTC 1 cut(s) 860
AccBSI CCGCTC 1 cut(s) 329
AccI GTMKAC 2 cut(s) 122, 768
AccII CGCG 1 cut(s) 236
AciI CCGC 3 cut(s) 236, 329, 1374
AclWI GGATC 3 cut(s) 688, 707, 1332
AcoI YGGCCR 2 cut(s) 63, 1157
AcsI RAATTY 2 cut(s) 52, 419
AcuI CTGAAG 1 cut(s) 1097
AcyI GRCGYC 2 cut(s) 95, 857
AfaI GTAC 6 cut(s) 227, 397, 510, 792, 920, 1299
AfiI CCNNNNNNNGG 4 cut(s) 104, 317, 1373, 1394
AflIII ACRYGT 1 cut(s) 1294
AgsI TTSAA 5 cut(s) 131, 518, 1208, 1246, 1292
AjnI CCWGG 4 cut(s) 211, 310, 688, 1012
AjuI GAANNNNNNNTTGG 2 cut(s) 44, 76
AloI GAACNNNNNNTCC 2 cut(s) 318, 350
Alw26I GTCTC 1 cut(s) 764
AlwI GGATC 3 cut(s) 688, 707, 1332
Ama87I CYCGRG 3 cut(s) 39, 391, 1136
AoxI GGCC 2 cut(s) 63, 1157
ApeKI GCWGC 3 cut(s) 569, 1040, 1400
ApoI RAATTY 2 cut(s) 52, 419
AseI ATTAAT 2 cut(s) 444, 654
Asp700I GAANNNNTTC 1 cut(s) 23
AspLEI GCGC 2 cut(s) 38, 317
AspS9I GGNCC 1 cut(s) 1364
AsuC2I CCSGG 1 cut(s) 1362
AsuHPI GGTGA 3 cut(s) 28, 229, 1070
AsuII TTCGAA 1 cut(s) 423
AvaI CYCGRG 3 cut(s) 39, 391, 1136
AvaII GGWCC 1 cut(s) 1364
BbvI GCAGC 3 cut(s) 556, 1052, 1387
BccI CCATC 1 cut(s) 1042
BceAI ACGGC 1 cut(s) 1172
BcgI CGANNNNNNTGC 2 cut(s) 551, 585
BciT130I CCWGG 4 cut(s) 213, 312, 690, 1014
BciVI GTATCC 2 cut(s) 828, 1111
BcnI CCSGG 1 cut(s) 1362
BcoDI GTCTC 1 cut(s) 764
BfaI CTAG 2 cut(s) 744, 1188
BfmI CTRYAG 2 cut(s) 606, 1401
BfoI RGCGCY 1 cut(s) 39
BfuI GTATCC 2 cut(s) 828, 1111
BisI GCNGC 3 cut(s) 570, 1041, 1401
BlsI GCNGC 3 cut(s) 571, 1042, 1402
Bme1390I CCNGG 5 cut(s) 213, 312, 690, 1014, 1362
Bme18I GGWCC 1 cut(s) 1364
BmeT110I CYCGRG 3 cut(s) 39, 391, 1136
BmgT120I GGNCC 1 cut(s) 1364
BmiI GGNNCC 3 cut(s) 210, 894, 1366
BmrFI CCNGG 5 cut(s) 213, 312, 690, 1014, 1362
BmsI GCATC 2 cut(s) 952, 1401
BoxI GACNNNNGTC 1 cut(s) 774
BplI GAGNNNNNCTC 2 cut(s) 440, 472
Bpu14I TTCGAA 1 cut(s) 423
BpuMI CCSGG 1 cut(s) 1362
BsaHI GRCGYC 2 cut(s) 95, 857
BsaJI CCNNGG 2 cut(s) 212, 928
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bsc4I CCNNNNNNNGG 4 cut(s) 104, 317, 1373, 1394
Bse118I RCCGGY 1 cut(s) 65
Bse3DI GCAATG 1 cut(s) 1250
BseBI CCWGG 4 cut(s) 213, 312, 690, 1014
BseDI CCNNGG 2 cut(s) 212, 928
BseGI GGATG 2 cut(s) 496, 1416
BseLI CCNNNNNNNGG 4 cut(s) 104, 317, 1373, 1394
BseMI GCAATG 1 cut(s) 1250
BseMII CTCAG 2 cut(s) 567, 1310
BseRI GAGGAG 1 cut(s) 737
BseXI GCAGC 3 cut(s) 556, 1052, 1387
BsgI GTGCAG 3 cut(s) 170, 555, 669
Bsh1236I CGCG 1 cut(s) 236
BshFI GGCC 2 cut(s) 65, 1159
BsiHKCI CYCGRG 3 cut(s) 39, 391, 1136
BsiSI CCGG 2 cut(s) 66, 1362
BslFI GGGAC 1 cut(s) 587
BslI CCNNNNNNNGG 4 cut(s) 104, 317, 1373, 1394
BsmAI GTCTC 1 cut(s) 764
BsmFI GGGAC 1 cut(s) 587
BsmI GAATGC 2 cut(s) 1252, 1269
BsnI GGCC 2 cut(s) 65, 1159
BsoBI CYCGRG 3 cut(s) 39, 391, 1136
Bsp119I TTCGAA 1 cut(s) 423
Bsp1407I TGTACA 2 cut(s) 508, 918
Bsp143I GATC 3 cut(s) 680, 712, 1324
Bsp19I CCATGG 1 cut(s) 928
BspACI CCGC 3 cut(s) 236, 329, 1374
BspANI GGCC 2 cut(s) 65, 1159
BspCNI CTCAG 2 cut(s) 566, 1311
BspFNI CGCG 1 cut(s) 236
BspHI TCATGA 1 cut(s) 189
BspLI GGNNCC 3 cut(s) 210, 894, 1366
BspMAI CTGCAG 1 cut(s) 1405
BspPI GGATC 3 cut(s) 688, 707, 1332
BspT104I TTCGAA 1 cut(s) 423
BsrBI CCGCTC 1 cut(s) 329
BsrDI GCAATG 1 cut(s) 1250
BsrFI RCCGGY 1 cut(s) 65
BsrGI TGTACA 2 cut(s) 508, 918
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 2 cut(s) 212, 928
BssMI GATC 3 cut(s) 680, 712, 1324
BssNI GRCGYC 2 cut(s) 95, 857
BssT1I CCWWGG 1 cut(s) 928
Bst2UI CCWGG 4 cut(s) 213, 312, 690, 1014
Bst4CI ACNGT 7 cut(s) 267, 400, 513, 667, 1171, 1348, 1397
Bst6I CTCTTC 1 cut(s) 116
BstACI GRCGYC 2 cut(s) 95, 857
BstAUI TGTACA 2 cut(s) 508, 918
BstBI TTCGAA 1 cut(s) 423
BstC8I GCNNGC 3 cut(s) 454, 1238, 1383
BstDEI CTNAG 4 cut(s) 140, 553, 1319, 1328
BstDSI CCRYGG 1 cut(s) 928
BstENI CCTNNNNNAGG 1 cut(s) 315
BstF5I GGATG 2 cut(s) 496, 1416
BstFNI CGCG 1 cut(s) 236
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 2 cut(s) 38, 317
BstKTI GATC 3 cut(s) 683, 715, 1327
BstMAI GTCTC 1 cut(s) 764
BstMBI GATC 3 cut(s) 680, 712, 1324
BstMWI GCNNNNNNNGC 3 cut(s) 554, 1040, 1049
BstNI CCWGG 4 cut(s) 213, 312, 690, 1014
BstNSI RCATGY 2 cut(s) 456, 1298
BstPAI GACNNNNGTC 1 cut(s) 774
BstSCI CCNGG 5 cut(s) 211, 310, 688, 1012, 1360
BstSFI CTRYAG 2 cut(s) 606, 1401
BstUI CGCG 1 cut(s) 236
BstV1I GCAGC 3 cut(s) 556, 1052, 1387
BstX2I RGATCY 2 cut(s) 680, 1324
BstYI RGATCY 2 cut(s) 680, 1324
BsuI GTATCC 2 cut(s) 828, 1111
BsuRI GGCC 2 cut(s) 65, 1159
BtgI CCRYGG 1 cut(s) 928
BtsCI GGATG 2 cut(s) 496, 1416
BtsIMutI CAGTG 1 cut(s) 663
Cac8I GCNNGC 3 cut(s) 454, 1238, 1383
CciI TCATGA 1 cut(s) 189
CfoI GCGC 2 cut(s) 38, 317
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 1 cut(s) 1364
CseI GACGC 4 cut(s) 103, 108, 133, 242
CsiI ACCWGGT 2 cut(s) 688, 1012
Csp6I GTAC 6 cut(s) 226, 396, 509, 791, 919, 1298
CviAII CATG 9 cut(s) 190, 453, 701, 764, 929, 1093, 1165, 1222, 1295
CviQI GTAC 6 cut(s) 226, 396, 509, 791, 919, 1298
DdeI CTNAG 4 cut(s) 140, 553, 1319, 1328
DpnI GATC 3 cut(s) 682, 714, 1326
DpnII GATC 3 cut(s) 680, 712, 1324
EaeI YGGCCR 2 cut(s) 63, 1157
Eam1104I CTCTTC 1 cut(s) 116
EarI CTCTTC 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 928
Eco32I GATATC 2 cut(s) 754, 1219
Eco47I GGWCC 1 cut(s) 1364
Eco57I CTGAAG 1 cut(s) 1097
Eco88I CYCGRG 3 cut(s) 39, 391, 1136
EcoNI CCTNNNNNAGG 1 cut(s) 315
EcoRI GAATTC 2 cut(s) 52, 419
EcoRII CCWGG 4 cut(s) 211, 310, 688, 1012
EcoRV GATATC 2 cut(s) 754, 1219
EcoT14I CCWWGG 1 cut(s) 928
EcoT22I ATGCAT 1 cut(s) 1252
ErhI CCWWGG 1 cut(s) 928
FaeI CATG 9 cut(s) 193, 456, 704, 767, 932, 1096, 1168, 1225, 1298
FaqI GGGAC 1 cut(s) 587
FatI CATG 9 cut(s) 189, 452, 700, 763, 928, 1092, 1164, 1221, 1294
FauI CCCGC 1 cut(s) 322
FauNDI CATATG 1 cut(s) 486
FblI GTMKAC 2 cut(s) 122, 768
Fnu4HI GCNGC 3 cut(s) 570, 1041, 1401
FokI GGATG 2 cut(s) 503, 1423
Fsp4HI GCNGC 3 cut(s) 570, 1041, 1401
FspBI CTAG 2 cut(s) 744, 1188
GlaI GCGC 2 cut(s) 37, 316
GluI GCNGC 3 cut(s) 570, 1041, 1401
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 2 cut(s) 65, 1159
HapII CCGG 2 cut(s) 66, 1362
HgaI GACGC 4 cut(s) 103, 108, 133, 242
HhaI GCGC 2 cut(s) 38, 317
Hin1I GRCGYC 2 cut(s) 95, 857
Hin1II CATG 9 cut(s) 193, 456, 704, 767, 932, 1096, 1168, 1225, 1298
Hin6I GCGC 2 cut(s) 36, 315
HinP1I GCGC 2 cut(s) 36, 315
HincII GTYRAC 3 cut(s) 123, 166, 769
HindII GTYRAC 3 cut(s) 123, 166, 769
HindIII AAGCTT 1 cut(s) 809
HinfI GANTC 8 cut(s) 5, 108, 162, 542, 648, 823, 1334, 1424
HpaII CCGG 2 cut(s) 66, 1362
HphI GGTGA 3 cut(s) 28, 229, 1070
Hpy166II GTNNAC 3 cut(s) 123, 166, 769
Hpy188I TCNGA 4 cut(s) 102, 822, 1116, 1320
Hpy188III TCNNGA 6 cut(s) 190, 338, 499, 972, 1136, 1307
Hpy8I GTNNAC 3 cut(s) 123, 166, 769
Hpy99I CGWCG 4 cut(s) 16, 97, 124, 127
HpyAV CCTTC 3 cut(s) 151, 419, 1058
HpyCH4III ACNGT 7 cut(s) 267, 400, 513, 667, 1171, 1348, 1397
HpyCH4IV ACGT 1 cut(s) 857
HpyF10VI GCNNNNNNNGC 3 cut(s) 554, 1040, 1049
HpyF3I CTNAG 4 cut(s) 140, 553, 1319, 1328
HpySE526I ACGT 1 cut(s) 857
Hsp92I GRCGYC 2 cut(s) 95, 857
Hsp92II CATG 9 cut(s) 193, 456, 704, 767, 932, 1096, 1168, 1225, 1298
HspAI GCGC 2 cut(s) 36, 315
Kzo9I GATC 3 cut(s) 680, 712, 1324
LmnI GCTCC 4 cut(s) 326, 724, 898, 1390
Lsp1109I GCAGC 3 cut(s) 556, 1052, 1387
LweI GCATC 2 cut(s) 952, 1401
MabI ACCWGGT 2 cut(s) 688, 1012
MaeI CTAG 2 cut(s) 744, 1188
MaeII ACGT 1 cut(s) 857
MaeIII GTNAC 4 cut(s) 68, 275, 853, 958
MalI GATC 3 cut(s) 682, 714, 1326
MbiI CCGCTC 1 cut(s) 329
MboI GATC 3 cut(s) 680, 712, 1324
MboII GAAGA 3 cut(s) 103, 232, 657
MflI RGATCY 2 cut(s) 680, 1324
MlyI GAGTC 2 cut(s) 102, 171
MmeI TCCRAC 2 cut(s) 658, 1016
Mph1103I ATGCAT 1 cut(s) 1252
MroXI GAANNNNTTC 1 cut(s) 23
MseI TTAA 6 cut(s) 440, 444, 582, 654, 846, 1022
MslI CAYNNNNRTG 3 cut(s) 489, 882, 1245
MspA1I CMGCKG 1 cut(s) 869
MspI CCGG 2 cut(s) 66, 1362
MspR9I CCNGG 5 cut(s) 213, 312, 690, 1014, 1362
Mva1269I GAATGC 2 cut(s) 1252, 1269
MvaI CCWGG 4 cut(s) 213, 312, 690, 1014
MvnI CGCG 1 cut(s) 236
MwoI GCNNNNNNNGC 3 cut(s) 554, 1040, 1049
NciI CCSGG 1 cut(s) 1362
NcoI CCATGG 1 cut(s) 928
NdeI CATATG 1 cut(s) 486
NdeII GATC 3 cut(s) 680, 712, 1324
NlaIII CATG 9 cut(s) 193, 456, 704, 767, 932, 1096, 1168, 1225, 1298
NlaIV GGNNCC 3 cut(s) 210, 894, 1366
NmeAIII GCCGAG 1 cut(s) 12
NmuCI GTSAC 1 cut(s) 853
NsiI ATGCAT 1 cut(s) 1252
NspI RCATGY 2 cut(s) 456, 1298
NspV TTCGAA 1 cut(s) 423
PacI TTAATTAA 1 cut(s) 444
PaeI GCATGC 1 cut(s) 456
PaeR7I CTCGAG 1 cut(s) 39
PagI TCATGA 1 cut(s) 189
PciI ACATGT 1 cut(s) 1294
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 53
PctI GAATGC 2 cut(s) 1252, 1269
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 6 cut(s) 5, 542, 648, 823, 1334, 1424
PfoI TCCNGGA 1 cut(s) 1360
PkrI GCNGC 3 cut(s) 571, 1042, 1402
PleI GAGTC 2 cut(s) 102, 170
PpsI GAGTC 2 cut(s) 102, 170
PscI ACATGT 1 cut(s) 1294
PshAI GACNNNNGTC 1 cut(s) 774
PshBI ATTAAT 2 cut(s) 444, 654
PsiI TTATAA 1 cut(s) 1441
Psp6I CCWGG 4 cut(s) 211, 310, 688, 1012
PspGI CCWGG 4 cut(s) 211, 310, 688, 1012
PspN4I GGNNCC 3 cut(s) 210, 894, 1366
PspPI GGNCC 1 cut(s) 1364
PspXI VCTCGAGB 1 cut(s) 39
PstI CTGCAG 1 cut(s) 1405
PsuI RGATCY 2 cut(s) 680, 1324
PvuII CAGCTG 1 cut(s) 869
RsaI GTAC 6 cut(s) 227, 397, 510, 792, 920, 1299
RsaNI GTAC 6 cut(s) 226, 396, 509, 791, 919, 1298
RseI CAYNNNNRTG 3 cut(s) 489, 882, 1245
SalI GTCGAC 2 cut(s) 121, 767
SaqAI TTAA 6 cut(s) 440, 444, 582, 654, 846, 1022
SatI GCNGC 3 cut(s) 570, 1041, 1401
Sau3AI GATC 3 cut(s) 680, 712, 1324
Sau96I GGNCC 1 cut(s) 1364
SchI GAGTC 2 cut(s) 102, 171
ScrFI CCNGG 5 cut(s) 213, 312, 690, 1014, 1362
SexAI ACCWGGT 2 cut(s) 688, 1012
SfaNI GCATC 2 cut(s) 952, 1401
SfcI CTRYAG 2 cut(s) 606, 1401
Sfr274I CTCGAG 1 cut(s) 39
SfuI TTCGAA 1 cut(s) 423
SgrDI CGTCGACG 1 cut(s) 121
SinI GGWCC 1 cut(s) 1364
SlaI CTCGAG 1 cut(s) 39
SmiMI CAYNNNNRTG 3 cut(s) 489, 882, 1245
SmlI CTYRAG 1 cut(s) 39
SmoI CTYRAG 1 cut(s) 39
SphI GCATGC 1 cut(s) 456
SsiI CCGC 3 cut(s) 236, 329, 1374
SspI AATATT 1 cut(s) 578
SspMI CTAG 2 cut(s) 744, 1188
StyD4I CCNGG 5 cut(s) 211, 310, 688, 1012, 1360
StyI CCWWGG 1 cut(s) 928
TaaI ACNGT 7 cut(s) 267, 400, 513, 667, 1171, 1348, 1397
TaiI ACGT 1 cut(s) 860
TaqI TCGA 5 cut(s) 40, 92, 122, 423, 768
TatI WGTACW 4 cut(s) 225, 508, 790, 918
TfiI GAWTC 6 cut(s) 5, 542, 648, 823, 1334, 1424
Tru1I TTAA 6 cut(s) 440, 444, 582, 654, 846, 1022
Tru9I TTAA 6 cut(s) 440, 444, 582, 654, 846, 1022
TscAI CASTG 1 cut(s) 670
TseFI GTSAC 1 cut(s) 853
TseI GCWGC 3 cut(s) 569, 1040, 1400
Tsp45I GTSAC 1 cut(s) 853
TspDTI ATGAA 7 cut(s) 178, 187, 362, 640, 1135, 1262, 1416
TspGWI ACGGA 1 cut(s) 977
TspRI CASTG 1 cut(s) 670
VpaK11BI GGWCC 1 cut(s) 1364
VspI ATTAAT 2 cut(s) 444, 654
XagI CCTNNNNNAGG 1 cut(s) 315
XapI RAATTY 2 cut(s) 52, 419
XceI RCATGY 2 cut(s) 456, 1298
XhoI CTCGAG 1 cut(s) 39
XmiI GTMKAC 2 cut(s) 122, 768
XmnI GAANNNNTTC 1 cut(s) 23
XspI CTAG 2 cut(s) 744, 1188
ZraI GACGTC 1 cut(s) 858
Zsp2I ATGCAT 1 cut(s) 1252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.