MD13G1122300.v1.1

Sphingosine-1-phosphate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011402
Physical Location & Seq
Forward (+)
9024296 .. 9028972
4677 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1122300.v1.1.491

Sequence Viewer

Length: 1626 bp
ATGGATTCTGCTTCTGTGAAAACGTTCCTGCTCGATGTTAGAGCTTCTGCGAATTCGTTGTTGTCGCAGTACGAGCCTCTGGTTCTGGTTTCTGCTCCTCTCTTTGCTCTCCTGGTCGCTTCCACGCTTCAATCGGCTATCCATGGCCTGCAGGAGAAGGGACTCAAAGCCACACTCCTAGGGTTCTTCATGAGCTTCATCAAGTTGGTTCCTGGCGTCAAGAGTTACATCGACGCTGAGAAGCAAAAGGTTGTGGATAAGTTACAGTCTGGTGGTAAATCTGGAAGAGAAAGTTGGAGAACTGAATTGCCTAGCTCGGGGTTGGGAGTGGGAGTCATAGAGAAAATGAAAGATGAGAAAAGTAAAGATGTGGCTTGGCAAGGAAAATGCTCGGGTGCAGTTTACATTGGGGGAAGCGAGGTTGAAGGACATTTTTCTGTAATTAATGAGGCATGCTCAGTGTTTGCACATACCAATCCATTGCATATGGATGTCTTCAAAAGTGTGGTACGGTTTGAATCGGAAGTTGTTGCAATGACTGCTGCACTGCTTGGAAGTAAAAAGAAGTCTTCTGGAGGACAAATATGTGGAAACATGACATCCGGAGGAACTGAAAGTATATTATTAGCTGTGAAATCATCACGAGATTATATGAAAACCAGGAAGGGGATTAAGAAACCTGAAATGATAATACCTGAATCTGCTCACTCTGCATATGACAAGGCTGCACAATATTTTAACATCAAGCTCTGGCGTGTCCCTGTAAATAAAGAATTTCAAGCAGATGTCAAAGCAATTAGAAGACACATTAATAAGAACACCGTTTTGATTGTGGGATCTGCACCTGGTTTTCCTCATGGCATTGTTGATCCTATTGAGGAGCTTGGTCAATTGGCTTCTAGCTTTGACATCTGTCTGCATGTTGACCTTTGTCTTGGCGGCTTCGTATTACCTTTTGCTAATAAGCTCGGGTATCCAATTCCACCCTTTGATTTTTCAGTGAAAGGAGTGACTTCAATATCAGCAGATGTACATAAATATGGGCTGGCTCCTAAAGGAACTAGCACAGTTCTGTACAGAAACCATGACATCAGAAAGCATCAATTTGTTGCTGTTACCGAGTGGTCCGGAGGGCTATACGTGTCTCCAACCATAGCCGGAAGCAGGCCTGGTGGCTTGATTGCTGGGGCATGGGCAGCTATGATGTCTTTAGGGGAGGAAGGGTACTTGGAAAACACAAAAAACATCATGGAAGCATCAAAAAGACTTCAGAAAGGAATAGAGGAAATTCCGGAGCTGTTTATCATTGGAAAGCCAGACATGACAGTTGTAGCATGGGGATCTAATGTTGTTGACATATTTGAAGTCAATGATATCATGTCATCTAAAGGCTGGCATTTGAATGCATTGCAAAGACCCAACAGTATTCATATCTGCGTGACGCTTCAACATGTACCCGTCATTGATGACTTTCTCAGGGATCTAAGGGGATCCGTGAAAACTGTCAAAGAAAATCCAGGACCGATAAGCGGAGGGCTAGCTCCCATTTATGGTGCTGCAGGGAGGATGCCGGACAGAGGTATGGTTCAGGAGTTGCTGGTAAATTACATGGACGGTACATGCTAG

Protein Analysis

542

Amino Acids

58.57

Weight (kDa)

8.6

Isoelectric Point (pI)

42.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyridoxal_deC PF00282 157 - 445 2e-21 Pyridoxal-dependent decarboxylase conserved domain
Aminotran_5 PF00266 200 - 437 1.5e-08 Aminotransferase class-V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011402)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27980
fragaria_vesca FvH4_4g23470 FvH4_4g23470 FvH4_4g23470 FvH4_4g23470 FvH4_4g23470 FvH4_4g23470 FvH4_4g23470
malus_domestica MD13G1122300.v1.1
prunus_persica Prupe.1G222900_v2.0.a1
pyrus_communis pycom13g10640
rosa_chinensis RchiOBHm_Chr4g0429841
rosa_laevigata RLG00000007039
rosa_multiflora Rmu_sc0006762.1_g000006
rosa_roxburghii Rroxscaffold_5G00371600
rosa_rugosa Rorug04G0238500
rosa_samantha Rh4AG294900 Rh4BG301300 Rh4DG298100
rosa_wichuraiana Rw4G025510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 3 cut(s) 602, 1127, 1291
AciI CCGC 2 cut(s) 939, 1530
AclI AACGTT 1 cut(s) 23
AclWI GGATC 6 cut(s) 844, 863, 1348, 1485, 1488, 1498
AcsI RAATTY 3 cut(s) 52, 773, 1287
AcuI CTGAAG 1 cut(s) 1253
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 7 cut(s) 71, 510, 1032, 1076, 1226, 1455, 1618
AfiI CCNNNNNNNGG 6 cut(s) 317, 666, 1164, 1529, 1550, 1577
AflIII ACRYGT 2 cut(s) 1140, 1450
AgsI TTSAA 9 cut(s) 131, 425, 499, 518, 779, 1017, 1364, 1402, 1448
AjnI CCWGG 6 cut(s) 111, 211, 659, 844, 1168, 1516
AjuI GAANNNNNNNTTGG 2 cut(s) 277, 309
Alw26I GTCTC 1 cut(s) 1149
AlwI GGATC 6 cut(s) 844, 863, 1348, 1485, 1488, 1498
Ama87I CYCGRG 3 cut(s) 316, 391, 968
Aor13HI TCCGGA 3 cut(s) 602, 1127, 1291
AoxI GGCC 2 cut(s) 145, 1166
ApeKI GCWGC 4 cut(s) 542, 725, 1196, 1556
ApoI RAATTY 3 cut(s) 52, 773, 1287
AseI ATTAAT 2 cut(s) 444, 810
Asp700I GAANNNNTTC 1 cut(s) 23
AspA2I CCTAGG 1 cut(s) 178
AspS9I GGNCC 2 cut(s) 1125, 1520
AsuNHI GCTAGC 1 cut(s) 1537
AvaI CYCGRG 3 cut(s) 316, 391, 968
AvaII GGWCC 2 cut(s) 1125, 1520
AvrII CCTAGG 1 cut(s) 178
BamHI GGATCC 1 cut(s) 1490
BauI CACGAG 1 cut(s) 642
BbsI GAAGAC 3 cut(s) 487, 561, 808
BbvI GCAGC 4 cut(s) 529, 712, 1208, 1543
BciT130I CCWGG 6 cut(s) 113, 213, 661, 846, 1170, 1518
BciVI GTATCC 1 cut(s) 984
BcoDI GTCTC 1 cut(s) 1149
BfaI CTAG 6 cut(s) 179, 312, 900, 1062, 1538, 1624
BfmI CTRYAG 2 cut(s) 149, 1557
BfuI GTATCC 1 cut(s) 984
BisI GCNGC 5 cut(s) 543, 726, 940, 1197, 1557
BlnI CCTAGG 1 cut(s) 178
BlsI GCNGC 5 cut(s) 544, 727, 941, 1198, 1558
Bme1390I CCNGG 6 cut(s) 113, 213, 661, 846, 1170, 1518
Bme18I GGWCC 2 cut(s) 1125, 1520
BmeT110I CYCGRG 3 cut(s) 316, 391, 968
BmgT120I GGNCC 2 cut(s) 1125, 1520
BmiI GGNNCC 3 cut(s) 210, 1050, 1492
BmrFI CCNGG 6 cut(s) 113, 213, 661, 846, 1170, 1518
BmsI GCATC 3 cut(s) 1108, 1265, 1557
BmtI GCTAGC 1 cut(s) 1541
BoxI GACNNNNGTC 2 cut(s) 912, 930
BpiI GAAGAC 3 cut(s) 487, 561, 808
BplI GAGNNNNNCTC 2 cut(s) 440, 472
BpmI CTGGAG 1 cut(s) 594
BsaAI YACGTR 1 cut(s) 1141
BsaHI GRCGYC 1 cut(s) 216
BsaJI CCNNGG 2 cut(s) 142, 178
BsaWI WCCGGW 3 cut(s) 602, 1127, 1291
BsaXI ACNNNNNCTCC 4 cut(s) 318, 348, 1208, 1238
Bsc4I CCNNNNNNNGG 6 cut(s) 317, 666, 1164, 1529, 1550, 1577
Bse3DI GCAATG 3 cut(s) 479, 540, 1406
BseAI TCCGGA 3 cut(s) 602, 1127, 1291
BseBI CCWGG 6 cut(s) 113, 213, 661, 846, 1170, 1518
BseDI CCNNGG 2 cut(s) 142, 178
BseGI GGATG 3 cut(s) 496, 599, 1572
BseLI CCNNNNNNNGG 6 cut(s) 317, 666, 1164, 1529, 1550, 1577
BseMI GCAATG 3 cut(s) 479, 540, 1406
BseMII CTCAG 3 cut(s) 228, 471, 1489
BseRI GAGGAG 2 cut(s) 87, 893
BseXI GCAGC 4 cut(s) 529, 712, 1208, 1543
BseYI CCCAGC 1 cut(s) 1184
BsgI GTGCAG 4 cut(s) 417, 528, 711, 825
BshFI GGCC 2 cut(s) 147, 1168
BsiHKCI CYCGRG 3 cut(s) 316, 391, 968
BsiSI CCGG 5 cut(s) 603, 1128, 1158, 1292, 1571
BslFI GGGAC 2 cut(s) 174, 743
BslI CCNNNNNNNGG 6 cut(s) 317, 666, 1164, 1529, 1550, 1577
BsmAI GTCTC 1 cut(s) 1149
BsmFI GGGAC 2 cut(s) 174, 743
BsmI GAATGC 1 cut(s) 1408
BsnI GGCC 2 cut(s) 147, 1168
BsoBI CYCGRG 3 cut(s) 316, 391, 968
Bsp13I TCCGGA 3 cut(s) 602, 1127, 1291
Bsp1407I TGTACA 2 cut(s) 1030, 1074
Bsp143I GATC 5 cut(s) 836, 868, 1340, 1480, 1490
Bsp19I CCATGG 1 cut(s) 142
BspACI CCGC 2 cut(s) 939, 1530
BspANI GGCC 2 cut(s) 147, 1168
BspCNI CTCAG 3 cut(s) 229, 470, 1488
BspEI TCCGGA 3 cut(s) 602, 1127, 1291
BspHI TCATGA 1 cut(s) 189
BspLI GGNNCC 3 cut(s) 210, 1050, 1492
BspMAI CTGCAG 2 cut(s) 153, 1561
BspOI GCTAGC 1 cut(s) 1541
BspPI GGATC 6 cut(s) 844, 863, 1348, 1485, 1488, 1498
BsrDI GCAATG 3 cut(s) 479, 540, 1406
BsrGI TGTACA 2 cut(s) 1030, 1074
BssECI CCNNGG 2 cut(s) 142, 178
BssMI GATC 5 cut(s) 836, 868, 1340, 1480, 1490
BssNI GRCGYC 1 cut(s) 216
BssSI CACGAG 1 cut(s) 642
BssT1I CCWWGG 2 cut(s) 142, 178
Bst2BI CACGAG 1 cut(s) 642
Bst2UI CCWGG 6 cut(s) 113, 213, 661, 846, 1170, 1518
Bst4CI ACNGT 8 cut(s) 267, 513, 823, 1069, 1327, 1424, 1504, 1616
Bst6I CTCTTC 1 cut(s) 280
BstACI GRCGYC 1 cut(s) 216
BstAPI GCANNNNNTGC 1 cut(s) 539
BstAUI TGTACA 2 cut(s) 1030, 1074
BstBAI YACGTR 1 cut(s) 1141
BstC8I GCNNGC 6 cut(s) 149, 454, 1047, 1166, 1394, 1539
BstDEI CTNAG 4 cut(s) 237, 457, 1475, 1484
BstDSI CCRYGG 1 cut(s) 142
BstF5I GGATG 3 cut(s) 496, 599, 1572
BstKTI GATC 5 cut(s) 839, 871, 1343, 1483, 1493
BstMAI GTCTC 1 cut(s) 1149
BstMBI GATC 5 cut(s) 836, 868, 1340, 1480, 1490
BstMWI GCNNNNNNNGC 4 cut(s) 73, 539, 710, 1196
BstNI CCWGG 6 cut(s) 113, 213, 661, 846, 1170, 1518
BstNSI RCATGY 4 cut(s) 456, 923, 1454, 1623
BstPAI GACNNNNGTC 2 cut(s) 912, 930
BstSCI CCNGG 6 cut(s) 111, 211, 659, 844, 1168, 1516
BstSFI CTRYAG 2 cut(s) 149, 1557
BstV1I GCAGC 4 cut(s) 529, 712, 1208, 1543
BstV2I GAAGAC 3 cut(s) 487, 561, 808
BstX2I RGATCY 4 cut(s) 836, 1340, 1480, 1490
BstYI RGATCY 4 cut(s) 836, 1340, 1480, 1490
BsuI GTATCC 1 cut(s) 984
BsuRI GGCC 2 cut(s) 147, 1168
BtgI CCRYGG 1 cut(s) 142
BtsCI GGATG 3 cut(s) 496, 599, 1572
BtsI GCAGTG 1 cut(s) 545
BtsIMutI CAGTG 3 cut(s) 465, 545, 1005
Cac8I GCNNGC 6 cut(s) 149, 454, 1047, 1166, 1394, 1539
CciI TCATGA 1 cut(s) 189
Cfr13I GGNCC 2 cut(s) 1125, 1520
CseI GACGC 3 cut(s) 205, 242, 1450
CsiI ACCWGGT 1 cut(s) 844
Csp6I GTAC 7 cut(s) 70, 509, 1031, 1075, 1225, 1454, 1617
CviQI GTAC 7 cut(s) 70, 509, 1031, 1075, 1225, 1454, 1617
DdeI CTNAG 4 cut(s) 237, 457, 1475, 1484
DpnI GATC 5 cut(s) 838, 870, 1342, 1482, 1492
DpnII GATC 5 cut(s) 836, 868, 1340, 1480, 1490
Eam1104I CTCTTC 1 cut(s) 280
EarI CTCTTC 1 cut(s) 280
Eco130I CCWWGG 2 cut(s) 142, 178
Eco147I AGGCCT 1 cut(s) 1168
Eco32I GATATC 1 cut(s) 1375
Eco47I GGWCC 2 cut(s) 1125, 1520
Eco57I CTGAAG 1 cut(s) 1253
Eco88I CYCGRG 3 cut(s) 316, 391, 968
EcoRI GAATTC 1 cut(s) 52
EcoRII CCWGG 6 cut(s) 111, 211, 659, 844, 1168, 1516
EcoRV GATATC 1 cut(s) 1375
EcoT14I CCWWGG 2 cut(s) 142, 178
EcoT22I ATGCAT 1 cut(s) 1408
ErhI CCWWGG 2 cut(s) 142, 178
FaqI GGGAC 2 cut(s) 174, 743
FauNDI CATATG 2 cut(s) 486, 715
Fnu4HI GCNGC 5 cut(s) 543, 726, 940, 1197, 1557
FokI GGATG 3 cut(s) 503, 586, 1579
Fsp4HI GCNGC 5 cut(s) 543, 726, 940, 1197, 1557
FspBI CTAG 6 cut(s) 179, 312, 900, 1062, 1538, 1624
GluI GCNGC 5 cut(s) 543, 726, 940, 1197, 1557
GsaI CCCAGC 1 cut(s) 1188
GsuI CTGGAG 1 cut(s) 594
HaeIII GGCC 2 cut(s) 147, 1168
HapII CCGG 5 cut(s) 603, 1128, 1158, 1292, 1571
HgaI GACGC 3 cut(s) 205, 242, 1450
Hin1I GRCGYC 1 cut(s) 216
HincII GTYRAC 2 cut(s) 925, 1354
HindII GTYRAC 2 cut(s) 925, 1354
HinfI GANTC 5 cut(s) 5, 162, 333, 518, 698
HpaII CCGG 5 cut(s) 603, 1128, 1158, 1292, 1571
Hpy166II GTNNAC 3 cut(s) 403, 925, 1354
Hpy188I TCNGA 3 cut(s) 523, 1094, 1272
Hpy188III TCNNGA 9 cut(s) 190, 220, 282, 573, 603, 642, 1128, 1292, 1589
Hpy8I GTNNAC 3 cut(s) 403, 925, 1354
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 4 cut(s) 151, 419, 658, 1214
HpyCH4III ACNGT 8 cut(s) 267, 513, 823, 1069, 1327, 1424, 1504, 1616
HpyCH4IV ACGT 2 cut(s) 23, 1140
HpyF10VI GCNNNNNNNGC 4 cut(s) 73, 539, 710, 1196
HpyF3I CTNAG 4 cut(s) 237, 457, 1475, 1484
HpySE526I ACGT 2 cut(s) 23, 1140
Hsp92I GRCGYC 1 cut(s) 216
Kpn2I TCCGGA 3 cut(s) 602, 1127, 1291
Kzo9I GATC 5 cut(s) 836, 868, 1340, 1480, 1490
LmnI GCTCC 5 cut(s) 100, 880, 1054, 1294, 1546
Lsp1109I GCAGC 4 cut(s) 529, 712, 1208, 1543
LweI GCATC 3 cut(s) 1108, 1265, 1557
MabI ACCWGGT 1 cut(s) 844
MaeI CTAG 6 cut(s) 179, 312, 900, 1062, 1538, 1624
MaeII ACGT 2 cut(s) 23, 1140
MaeIII GTNAC 5 cut(s) 224, 261, 1009, 1114, 1438
MalI GATC 5 cut(s) 838, 870, 1342, 1482, 1492
MboI GATC 5 cut(s) 836, 868, 1340, 1480, 1490
MboII GAAGA 5 cut(s) 178, 297, 487, 561, 813
MfeI CAATTG 1 cut(s) 890
MflI RGATCY 4 cut(s) 836, 1340, 1480, 1490
MlyI GAGTC 2 cut(s) 156, 342
MmeI TCCRAC 2 cut(s) 275, 1172
Mph1103I ATGCAT 1 cut(s) 1408
MroI TCCGGA 3 cut(s) 602, 1127, 1291
MroXI GAANNNNTTC 1 cut(s) 23
MseI TTAA 4 cut(s) 444, 672, 738, 810
MslI CAYNNNNRTG 3 cut(s) 489, 1038, 1401
MspI CCGG 5 cut(s) 603, 1128, 1158, 1292, 1571
MspR9I CCNGG 6 cut(s) 113, 213, 661, 846, 1170, 1518
MunI CAATTG 1 cut(s) 890
Mva1269I GAATGC 1 cut(s) 1408
MvaI CCWGG 6 cut(s) 113, 213, 661, 846, 1170, 1518
MwoI GCNNNNNNNGC 4 cut(s) 73, 539, 710, 1196
NcoI CCATGG 1 cut(s) 142
NdeI CATATG 2 cut(s) 486, 715
NdeII GATC 5 cut(s) 836, 868, 1340, 1480, 1490
NheI GCTAGC 1 cut(s) 1537
NlaIV GGNNCC 3 cut(s) 210, 1050, 1492
NmuCI GTSAC 2 cut(s) 1009, 1438
NsiI ATGCAT 1 cut(s) 1408
NspI RCATGY 4 cut(s) 456, 923, 1454, 1623
PaeI GCATGC 1 cut(s) 456
PagI TCATGA 1 cut(s) 189
PceI AGGCCT 1 cut(s) 1168
PciI ACATGT 1 cut(s) 1450
PctI GAATGC 1 cut(s) 1408
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 3 cut(s) 5, 518, 698
PfoI TCCNGGA 1 cut(s) 1516
PkrI GCNGC 5 cut(s) 544, 727, 941, 1198, 1558
PleI GAGTC 2 cut(s) 156, 341
PpsI GAGTC 2 cut(s) 156, 341
Ppu21I YACGTR 1 cut(s) 1141
PscI ACATGT 1 cut(s) 1450
PshAI GACNNNNGTC 2 cut(s) 912, 930
PshBI ATTAAT 2 cut(s) 444, 810
Psp1406I AACGTT 1 cut(s) 23
Psp6I CCWGG 6 cut(s) 111, 211, 659, 844, 1168, 1516
PspFI CCCAGC 1 cut(s) 1184
PspGI CCWGG 6 cut(s) 111, 211, 659, 844, 1168, 1516
PspN4I GGNNCC 3 cut(s) 210, 1050, 1492
PspPI GGNCC 2 cut(s) 1125, 1520
PstI CTGCAG 2 cut(s) 153, 1561
PsuI RGATCY 4 cut(s) 836, 1340, 1480, 1490
RsaI GTAC 7 cut(s) 71, 510, 1032, 1076, 1226, 1455, 1618
RsaNI GTAC 7 cut(s) 70, 509, 1031, 1075, 1225, 1454, 1617
RseI CAYNNNNRTG 3 cut(s) 489, 1038, 1401
SaqAI TTAA 4 cut(s) 444, 672, 738, 810
SatI GCNGC 5 cut(s) 543, 726, 940, 1197, 1557
Sau3AI GATC 5 cut(s) 836, 868, 1340, 1480, 1490
Sau96I GGNCC 2 cut(s) 1125, 1520
SbfI CCTGCAGG 1 cut(s) 153
SchI GAGTC 2 cut(s) 156, 342
ScrFI CCNGG 6 cut(s) 113, 213, 661, 846, 1170, 1518
SdaI CCTGCAGG 1 cut(s) 153
SexAI ACCWGGT 1 cut(s) 844
SfaNI GCATC 3 cut(s) 1108, 1265, 1557
SfcI CTRYAG 2 cut(s) 149, 1557
SinI GGWCC 2 cut(s) 1125, 1520
SmiMI CAYNNNNRTG 3 cut(s) 489, 1038, 1401
SphI GCATGC 1 cut(s) 456
Sse8387I CCTGCAGG 1 cut(s) 153
SseBI AGGCCT 1 cut(s) 1168
SsiI CCGC 2 cut(s) 939, 1530
SspI AATATT 1 cut(s) 734
SspMI CTAG 6 cut(s) 179, 312, 900, 1062, 1538, 1624
StuI AGGCCT 1 cut(s) 1168
StyD4I CCNGG 6 cut(s) 111, 211, 659, 844, 1168, 1516
StyI CCWWGG 2 cut(s) 142, 178
TaaI ACNGT 8 cut(s) 267, 513, 823, 1069, 1327, 1424, 1504, 1616
TaiI ACGT 2 cut(s) 26, 1143
TaqI TCGA 2 cut(s) 33, 231
TaqII GACCGA 1 cut(s) 1537
TatI WGTACW 2 cut(s) 1030, 1074
TauI GCSGC 1 cut(s) 942
TfiI GAWTC 3 cut(s) 5, 518, 698
Tru1I TTAA 4 cut(s) 444, 672, 738, 810
Tru9I TTAA 4 cut(s) 444, 672, 738, 810
TscAI CASTG 3 cut(s) 465, 552, 1005
TseFI GTSAC 2 cut(s) 1009, 1438
TseI GCWGC 4 cut(s) 542, 725, 1196, 1556
Tsp45I GTSAC 2 cut(s) 1009, 1438
TspDTI ATGAA 5 cut(s) 178, 187, 362, 668, 1418
TspGWI ACGGA 1 cut(s) 1483
TspRI CASTG 3 cut(s) 465, 552, 1005
VpaK11BI GGWCC 2 cut(s) 1125, 1520
VspI ATTAAT 2 cut(s) 444, 810
XapI RAATTY 3 cut(s) 52, 773, 1287
XceI RCATGY 4 cut(s) 456, 923, 1454, 1623
XmaJI CCTAGG 1 cut(s) 178
XmnI GAANNNNTTC 1 cut(s) 23
XspI CTAG 6 cut(s) 179, 312, 900, 1062, 1538, 1624
Zsp2I ATGCAT 1 cut(s) 1408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.