Prupe.1G222900_v2.0.a1

Sphingosine-1-phosphate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0011402
Physical Location & Seq
Reverse (-)
23685609 .. 23690975
5367 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G222900.1

Sequence Viewer

Length: 1623 bp
ATGGATTCTTCTGTGAAATCGTTCCTGCTTGATGTTAGAGCTTCCGCGAATTCATTCTTGTCCGAATACGAGCCTCTGGTTCTGGTTTCTGCTCCTCTTATCGCACTCTTCGTTGCTTCCATGCTTCAATCGGCTCTCCAAGTCCTGCACGAGAACGGACTCAAAGCCACTCTGCTAGGGTTCTTCATGAGCTTCGTCAAGTTGGTTCCTGGCGTGAAGAGTTACATCGACGCTGAGAAACAAAAGGTTGTGGATAAGTTACAGTCTGGTGGTAAATCTGTAAGAGAAAGTTGGAGAACTGAATTGCCTAGCACAGGGTTGGGAGTGGGAGTCATAGAGCAAATGAAAGATGAGAAAAGAAAAGATGTGGCTTGGCAAGGGAAATGCTCTGGTGCAGTCTACATTGGAGGAAGTGAGTGTGAAGGACATTTTTCTTTAATTAATGAGGCATGCTCAATGTTTGCGCATACCAATCCATTGCATATGGATGTATTCCAGAGTGTGGTACGGTTTGAAGCAGAAGTGGTTGCAATGACTGCTGCAATGCTTGGAAGTAAAAAGAAGTCTTCTGGAGGACAAATATGTGGAAACATGACATCAGGAGGAACTGAAAGTATATTATTAGCTGTGAAATCATCACGAGATTATATGAAAACCACGAAGGGGATTAAAAAGCCTGAAATGATAATACCTGAATCTGCTCACTCAGCATACGACAAGGCTGCACAATATTTTAACATCAAGCTCTGGCGTGTCCCTGTAAATAAAGAATTTCAAGCAGACGTCAAAGCAATTAGAAGATACATTAATAGAAACACCGTTTTGATTGTTGGATCTGCACCTGGTTTCCCTCATGGCATTGTTGATCCGATTAAGGAGCTTGGTCAATTGGCTTCAAGCTTCGACATTTGTCTCCATGTTGACCTTTGTCTTGGCGGCTTCGTATTACCTTTTGCTAATAAGCTTGGGTATCCAATTCCACCCTTTGATTTTTCAGTGAAAGGAGTGACATCAATATCAGCGGATGTGCATAAATATGGGTTGGCTCCTAAAGGAACAAGTGTAGTTCTGTACAGAAACCATGACCTCAGAAAGCATCAATTTGTTGCCGTTACTGAGTGGTCAGGAGGGCTATATGTGTCTCCAACCATTGCCGGAAGCAGACCTGGTAGTTTAATTGCTGGGGCTTGGGCATCTATGATATCTTTGGGGGAGGAAGGGTACTTGGAAAACACAAAAAAGATCATGGAAGTATCAAAAAGACTTCAGAAAGGGATAGAGGAAATTCCCGAGTTGTTTGTCATTGGAAGGCCAGACATGACAGTTGTGGCATTGGGATCTAATGTTGTTGACATATTTGAAGTAAATGATATCATGTCATCTAAAGGCTGGCATTTGAATGCATTGCAAAGACCCAACAGCATTCATATCTGCGTGACGCTTCAACATGTACCAATTATTGATGACTTTCTCAGGGATCTAACGGAATCTGTGAAAACTGTTAAAGAAAATCCGGGTCCTATAAGCGGAGGGCTCGCTCCCATTTATGGTGCTGCGGGGAGGATGCCAGATAGAGGTACGGTTCAGGAGCTGTTGGTAAATTACATGGACGGTACATGCTAG

Protein Analysis

541

Amino Acids

58.75

Weight (kDa)

8.0

Isoelectric Point (pI)

38.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011402)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27980
fragaria_vesca FvH4_4g23470 FvH4_4g23470 FvH4_4g23470 FvH4_4g23470 FvH4_4g23470 FvH4_4g23470 FvH4_4g23470
malus_domestica MD13G1122300.v1.1
prunus_persica Prupe.1G222900_v2.0.a1
pyrus_communis pycom13g10640
rosa_chinensis RchiOBHm_Chr4g0429841
rosa_laevigata RLG00000007039
rosa_multiflora Rmu_sc0006762.1_g000006
rosa_roxburghii Rroxscaffold_5G00371600
rosa_rugosa Rorug04G0238500
rosa_samantha Rh4AG294900 Rh4BG301300 Rh4DG298100
rosa_wichuraiana Rw4G025510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 786
Acc16I TGCGCA 1 cut(s) 465
AccB7I CCANNNNNTGG 1 cut(s) 502
AccI GTMKAC 1 cut(s) 399
AccII CGCG 1 cut(s) 47
AciI CCGC 5 cut(s) 45, 936, 1022, 1527, 1556
AclWI GGATC 4 cut(s) 841, 860, 1345, 1485
AcsI RAATTY 3 cut(s) 49, 770, 1284
AcuI CTGAAG 1 cut(s) 1250
AcyI GRCGYC 1 cut(s) 783
AfaI GTAC 6 cut(s) 507, 1073, 1223, 1452, 1579, 1615
AfiI CCNNNNNNNGG 6 cut(s) 314, 502, 663, 1526, 1547, 1574
AflIII ACRYGT 1 cut(s) 1447
AgsI TTSAA 7 cut(s) 128, 515, 776, 897, 1361, 1399, 1445
AjnI CCWGG 3 cut(s) 208, 841, 1165
AluBI AGCT 8 cut(s) 41, 192, 626, 745, 880, 900, 964, 1591
AluI AGCT 8 cut(s) 41, 192, 626, 745, 880, 900, 964, 1591
Alw26I GTCTC 2 cut(s) 917, 1146
AlwI GGATC 4 cut(s) 841, 860, 1345, 1485
AlwNI CAGNNNCTG 1 cut(s) 1591
Ama87I CYCGRG 1 cut(s) 1289
AoxI GGCC 1 cut(s) 1310
ApeKI GCWGC 3 cut(s) 539, 722, 1553
ApoI RAATTY 3 cut(s) 49, 770, 1284
AseI ATTAAT 2 cut(s) 441, 807
Asp700I GAANNNNTTC 2 cut(s) 20, 53
AspLEI GCGC 1 cut(s) 466
AspS9I GGNCC 1 cut(s) 1517
AsuC2I CCSGG 1 cut(s) 1515
AvaI CYCGRG 1 cut(s) 1289
AvaII GGWCC 1 cut(s) 1517
BanII GRGCYC 1 cut(s) 1536
BauI CACGAG 2 cut(s) 149, 639
BbsI GAAGAC 1 cut(s) 558
BbvI GCAGC 3 cut(s) 526, 709, 1540
BceAI ACGGC 1 cut(s) 1094
BcgI CGANNNNNNTGC 2 cut(s) 704, 738
BciT130I CCWGG 3 cut(s) 210, 843, 1167
BciVI GTATCC 1 cut(s) 981
BcnI CCSGG 1 cut(s) 1515
BcoDI GTCTC 2 cut(s) 917, 1146
BfaI CTAG 3 cut(s) 176, 309, 1621
BfuI GTATCC 1 cut(s) 981
BisI GCNGC 4 cut(s) 540, 723, 937, 1554
BlsI GCNGC 4 cut(s) 541, 724, 938, 1555
Bme1390I CCNGG 4 cut(s) 210, 843, 1167, 1515
Bme18I GGWCC 1 cut(s) 1517
BmeT110I CYCGRG 1 cut(s) 1289
BmgT120I GGNCC 1 cut(s) 1517
BmiI GGNNCC 3 cut(s) 207, 1047, 1518
BmrFI CCNGG 4 cut(s) 210, 843, 1167, 1515
BmsI GCATC 3 cut(s) 1105, 1202, 1554
BoxI GACNNNNGTC 2 cut(s) 909, 927
BpiI GAAGAC 1 cut(s) 558
BplI GAGNNNNNCTC 2 cut(s) 437, 469
BpmI CTGGAG 1 cut(s) 591
BpuMI CCSGG 1 cut(s) 1515
BsaHI GRCGYC 1 cut(s) 783
BsaXI ACNNNNNCTCC 4 cut(s) 315, 345, 1205, 1235
Bsc4I CCNNNNNNNGG 6 cut(s) 314, 502, 663, 1526, 1547, 1574
Bse3DI GCAATG 5 cut(s) 476, 537, 549, 1149, 1403
BseBI CCWGG 3 cut(s) 210, 843, 1167
BseGI GGATG 3 cut(s) 493, 1030, 1569
BseLI CCNNNNNNNGG 6 cut(s) 314, 502, 663, 1526, 1547, 1574
BseMI GCAATG 5 cut(s) 476, 537, 549, 1149, 1403
BseMII CTCAG 5 cut(s) 225, 720, 1102, 1107, 1486
BseRI GAGGAG 1 cut(s) 84
BseXI GCAGC 3 cut(s) 526, 709, 1540
BseYI CCCAGC 1 cut(s) 1181
BsgI GTGCAG 4 cut(s) 131, 414, 708, 822
Bsh1236I CGCG 1 cut(s) 47
BshFI GGCC 1 cut(s) 1312
BsiHKCI CYCGRG 1 cut(s) 1289
BsiSI CCGG 2 cut(s) 1155, 1514
BslFI GGGAC 1 cut(s) 740
BslI CCNNNNNNNGG 6 cut(s) 314, 502, 663, 1526, 1547, 1574
BsmAI GTCTC 2 cut(s) 917, 1146
BsmFI GGGAC 1 cut(s) 740
BsmI GAATGC 2 cut(s) 1405, 1422
BsnI GGCC 1 cut(s) 1312
BsoBI CYCGRG 1 cut(s) 1289
Bsp1286I GDGCHC 1 cut(s) 1536
Bsp1407I TGTACA 1 cut(s) 1071
Bsp143I GATC 5 cut(s) 833, 865, 1242, 1337, 1477
BspACI CCGC 5 cut(s) 45, 936, 1022, 1527, 1556
BspANI GGCC 1 cut(s) 1312
BspCNI CTCAG 5 cut(s) 226, 719, 1101, 1108, 1485
BspFNI CGCG 1 cut(s) 47
BspHI TCATGA 1 cut(s) 186
BspLI GGNNCC 3 cut(s) 207, 1047, 1518
BspPI GGATC 4 cut(s) 841, 860, 1345, 1485
BsrDI GCAATG 5 cut(s) 476, 537, 549, 1149, 1403
BsrGI TGTACA 1 cut(s) 1071
BssMI GATC 5 cut(s) 833, 865, 1242, 1337, 1477
BssNI GRCGYC 1 cut(s) 783
BssSI CACGAG 2 cut(s) 149, 639
Bst2BI CACGAG 2 cut(s) 149, 639
Bst2UI CCWGG 3 cut(s) 210, 843, 1167
Bst4CI ACNGT 7 cut(s) 264, 510, 820, 1324, 1501, 1582, 1613
Bst6I CTCTTC 2 cut(s) 113, 212
BstACI GRCGYC 1 cut(s) 783
BstAPI GCANNNNNTGC 1 cut(s) 536
BstAUI TGTACA 1 cut(s) 1071
BstC8I GCNNGC 3 cut(s) 451, 1391, 1536
BstDEI CTNAG 5 cut(s) 234, 706, 1088, 1116, 1472
BstENI CCTNNNNNAGG 1 cut(s) 312
BstF5I GGATG 3 cut(s) 493, 1030, 1569
BstFNI CGCG 1 cut(s) 47
BstHHI GCGC 1 cut(s) 466
BstKTI GATC 5 cut(s) 836, 868, 1245, 1340, 1480
BstMAI GTCTC 2 cut(s) 917, 1146
BstMBI GATC 5 cut(s) 833, 865, 1242, 1337, 1477
BstMWI GCNNNNNNNGC 2 cut(s) 536, 707
BstNI CCWGG 3 cut(s) 210, 843, 1167
BstNSI RCATGY 3 cut(s) 453, 1451, 1620
BstPAI GACNNNNGTC 2 cut(s) 909, 927
BstSCI CCNGG 4 cut(s) 208, 841, 1165, 1513
BstUI CGCG 1 cut(s) 47
BstV1I GCAGC 3 cut(s) 526, 709, 1540
BstV2I GAAGAC 1 cut(s) 558
BstX2I RGATCY 3 cut(s) 833, 1337, 1477
BstYI RGATCY 3 cut(s) 833, 1337, 1477
BsuI GTATCC 1 cut(s) 981
BsuRI GGCC 1 cut(s) 1312
BtsCI GGATG 3 cut(s) 493, 1030, 1569
BtsIMutI CAGTG 1 cut(s) 1002
Cac8I GCNNGC 3 cut(s) 451, 1391, 1536
CaiI CAGNNNCTG 1 cut(s) 1591
CciI TCATGA 1 cut(s) 186
CfoI GCGC 1 cut(s) 466
Cfr13I GGNCC 1 cut(s) 1517
CseI GACGC 2 cut(s) 239, 1447
CsiI ACCWGGT 2 cut(s) 841, 1165
Csp6I GTAC 6 cut(s) 506, 1072, 1222, 1451, 1578, 1614
CviQI GTAC 6 cut(s) 506, 1072, 1222, 1451, 1578, 1614
DdeI CTNAG 5 cut(s) 234, 706, 1088, 1116, 1472
DpnI GATC 5 cut(s) 835, 867, 1244, 1339, 1479
DpnII GATC 5 cut(s) 833, 865, 1242, 1337, 1477
Eam1104I CTCTTC 2 cut(s) 113, 212
EarI CTCTTC 2 cut(s) 113, 212
Eco24I GRGCYC 1 cut(s) 1536
Eco32I GATATC 2 cut(s) 1203, 1372
Eco47I GGWCC 1 cut(s) 1517
Eco57I CTGAAG 1 cut(s) 1250
Eco88I CYCGRG 1 cut(s) 1289
EcoNI CCTNNNNNAGG 1 cut(s) 312
EcoO109I RGGNCCY 1 cut(s) 1517
EcoRI GAATTC 1 cut(s) 49
EcoRII CCWGG 3 cut(s) 208, 841, 1165
EcoRV GATATC 2 cut(s) 1203, 1372
EcoT22I ATGCAT 1 cut(s) 1405
EcoT38I GRGCYC 1 cut(s) 1536
FaqI GGGAC 1 cut(s) 740
FauI CCCGC 1 cut(s) 1549
FauNDI CATATG 1 cut(s) 483
FblI GTMKAC 1 cut(s) 399
Fnu4HI GCNGC 4 cut(s) 540, 723, 937, 1554
FokI GGATG 3 cut(s) 500, 1037, 1576
FriOI GRGCYC 1 cut(s) 1536
Fsp4HI GCNGC 4 cut(s) 540, 723, 937, 1554
FspBI CTAG 3 cut(s) 176, 309, 1621
FspI TGCGCA 1 cut(s) 465
GlaI GCGC 1 cut(s) 465
GluI GCNGC 4 cut(s) 540, 723, 937, 1554
GsaI CCCAGC 1 cut(s) 1185
GsuI CTGGAG 1 cut(s) 591
HaeIII GGCC 1 cut(s) 1312
HapII CCGG 2 cut(s) 1155, 1514
HgaI GACGC 2 cut(s) 239, 1447
HhaI GCGC 1 cut(s) 466
Hin1I GRCGYC 1 cut(s) 783
Hin6I GCGC 1 cut(s) 464
HinP1I GCGC 1 cut(s) 464
HincII GTYRAC 2 cut(s) 922, 1351
HindII GTYRAC 2 cut(s) 922, 1351
HindIII AAGCTT 2 cut(s) 898, 962
HinfI GANTC 5 cut(s) 5, 159, 330, 695, 1487
HpaII CCGG 2 cut(s) 1155, 1514
Hpy166II GTNNAC 3 cut(s) 400, 922, 1351
Hpy188I TCNGA 4 cut(s) 64, 870, 1091, 1269
Hpy188III TCNNGA 8 cut(s) 187, 496, 570, 600, 639, 1125, 1289, 1586
Hpy8I GTNNAC 3 cut(s) 400, 922, 1351
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 4 cut(s) 416, 655, 1211, 1302
HpyCH4III ACNGT 7 cut(s) 264, 510, 820, 1324, 1501, 1582, 1613
HpyCH4IV ACGT 1 cut(s) 783
HpyF10VI GCNNNNNNNGC 2 cut(s) 536, 707
HpyF3I CTNAG 5 cut(s) 234, 706, 1088, 1116, 1472
HpySE526I ACGT 1 cut(s) 783
Hsp92I GRCGYC 1 cut(s) 783
HspAI GCGC 1 cut(s) 464
Kzo9I GATC 5 cut(s) 833, 865, 1242, 1337, 1477
LmnI GCTCC 5 cut(s) 97, 877, 1051, 1543, 1588
Lsp1109I GCAGC 3 cut(s) 526, 709, 1540
LweI GCATC 3 cut(s) 1105, 1202, 1554
MabI ACCWGGT 2 cut(s) 841, 1165
MaeI CTAG 3 cut(s) 176, 309, 1621
MaeII ACGT 1 cut(s) 783
MaeIII GTNAC 5 cut(s) 221, 258, 1006, 1111, 1435
MalI GATC 5 cut(s) 835, 867, 1244, 1339, 1479
MboI GATC 5 cut(s) 833, 865, 1242, 1337, 1477
MboII GAAGA 5 cut(s) 100, 175, 229, 558, 810
MfeI CAATTG 1 cut(s) 887
MflI RGATCY 3 cut(s) 833, 1337, 1477
MhlI GDGCHC 1 cut(s) 1536
MlyI GAGTC 2 cut(s) 153, 339
MmeI TCCRAC 3 cut(s) 272, 811, 1169
Mph1103I ATGCAT 1 cut(s) 1405
MroXI GAANNNNTTC 2 cut(s) 20, 53
MseI TTAA 8 cut(s) 437, 441, 669, 735, 807, 873, 1175, 1503
MslI CAYNNNNRTG 3 cut(s) 486, 1035, 1398
MspA1I CMGCKG 1 cut(s) 1022
MspI CCGG 2 cut(s) 1155, 1514
MspR9I CCNGG 4 cut(s) 210, 843, 1167, 1515
MunI CAATTG 1 cut(s) 887
Mva1269I GAATGC 2 cut(s) 1405, 1422
MvaI CCWGG 3 cut(s) 210, 843, 1167
MvnI CGCG 1 cut(s) 47
MwoI GCNNNNNNNGC 2 cut(s) 536, 707
NciI CCSGG 1 cut(s) 1515
NdeI CATATG 1 cut(s) 483
NdeII GATC 5 cut(s) 833, 865, 1242, 1337, 1477
NlaIV GGNNCC 3 cut(s) 207, 1047, 1518
NmuCI GTSAC 2 cut(s) 1006, 1435
NsbI TGCGCA 1 cut(s) 465
NsiI ATGCAT 1 cut(s) 1405
NspI RCATGY 3 cut(s) 453, 1451, 1620
PacI TTAATTAA 1 cut(s) 441
PaeI GCATGC 1 cut(s) 453
PagI TCATGA 1 cut(s) 186
PciI ACATGT 1 cut(s) 1447
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PctI GAATGC 2 cut(s) 1405, 1422
PdmI GAANNNNTTC 2 cut(s) 20, 53
PfeI GAWTC 3 cut(s) 5, 695, 1487
PflMI CCANNNNNTGG 1 cut(s) 502
PkrI GCNGC 4 cut(s) 541, 724, 938, 1555
PleI GAGTC 2 cut(s) 153, 338
PpsI GAGTC 2 cut(s) 153, 338
PpuMI RGGWCCY 1 cut(s) 1517
PscI ACATGT 1 cut(s) 1447
PshAI GACNNNNGTC 2 cut(s) 909, 927
PshBI ATTAAT 2 cut(s) 441, 807
Psp5II RGGWCCY 1 cut(s) 1517
Psp6I CCWGG 3 cut(s) 208, 841, 1165
PspFI CCCAGC 1 cut(s) 1181
PspGI CCWGG 3 cut(s) 208, 841, 1165
PspN4I GGNNCC 3 cut(s) 207, 1047, 1518
PspPI GGNCC 1 cut(s) 1517
PspPPI RGGWCCY 1 cut(s) 1517
PstNI CAGNNNCTG 1 cut(s) 1591
PsuI RGATCY 3 cut(s) 833, 1337, 1477
RsaI GTAC 6 cut(s) 507, 1073, 1223, 1452, 1579, 1615
RsaNI GTAC 6 cut(s) 506, 1072, 1222, 1451, 1578, 1614
RseI CAYNNNNRTG 3 cut(s) 486, 1035, 1398
SaqAI TTAA 8 cut(s) 437, 441, 669, 735, 807, 873, 1175, 1503
SatI GCNGC 4 cut(s) 540, 723, 937, 1554
Sau3AI GATC 5 cut(s) 833, 865, 1242, 1337, 1477
Sau96I GGNCC 1 cut(s) 1517
SchI GAGTC 2 cut(s) 153, 339
ScrFI CCNGG 4 cut(s) 210, 843, 1167, 1515
SduI GDGCHC 1 cut(s) 1536
SexAI ACCWGGT 2 cut(s) 841, 1165
SfaNI GCATC 3 cut(s) 1105, 1202, 1554
SinI GGWCC 1 cut(s) 1517
SmiMI CAYNNNNRTG 3 cut(s) 486, 1035, 1398
SphI GCATGC 1 cut(s) 453
SsiI CCGC 5 cut(s) 45, 936, 1022, 1527, 1556
SspI AATATT 1 cut(s) 731
SspMI CTAG 3 cut(s) 176, 309, 1621
StyD4I CCNGG 4 cut(s) 208, 841, 1165, 1513
TaaI ACNGT 7 cut(s) 264, 510, 820, 1324, 1501, 1582, 1613
TaiI ACGT 1 cut(s) 786
TaqI TCGA 2 cut(s) 228, 903
TatI WGTACW 1 cut(s) 1071
TauI GCSGC 1 cut(s) 939
TfiI GAWTC 3 cut(s) 5, 695, 1487
Tru1I TTAA 8 cut(s) 437, 441, 669, 735, 807, 873, 1175, 1503
Tru9I TTAA 8 cut(s) 437, 441, 669, 735, 807, 873, 1175, 1503
TscAI CASTG 1 cut(s) 1002
TseFI GTSAC 2 cut(s) 1006, 1435
TseI GCWGC 3 cut(s) 539, 722, 1553
Tsp45I GTSAC 2 cut(s) 1006, 1435
TspDTI ATGAA 5 cut(s) 42, 175, 359, 665, 1415
TspGWI ACGGA 2 cut(s) 171, 1499
TspRI CASTG 1 cut(s) 1002
Van91I CCANNNNNTGG 1 cut(s) 502
VpaK11BI GGWCC 1 cut(s) 1517
VspI ATTAAT 2 cut(s) 441, 807
XagI CCTNNNNNAGG 1 cut(s) 312
XapI RAATTY 3 cut(s) 49, 770, 1284
XceI RCATGY 3 cut(s) 453, 1451, 1620
XmiI GTMKAC 1 cut(s) 399
XmnI GAANNNNTTC 2 cut(s) 20, 53
XspI CTAG 3 cut(s) 176, 309, 1621
ZraI GACGTC 1 cut(s) 784
Zsp2I ATGCAT 1 cut(s) 1405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.