Rh4DG298100

Sphingosine-1-phosphate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0011402
Physical Location & Seq
Reverse (-)
51980507 .. 51986349
5843 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG298100.1

Sequence Viewer

Length: 1530 bp
ATGGATTCTTCGTCGGTGAAATCGTTCCTGCTCGGCGCTCGAGCTTCGGCGAATTCGTTTTTGGCCGGTTACGAGCCTCTGGTTCTGGTTTCGACGCCTCTGATTGGACTCTTCGTTGCGTCGACAATTCAATCGGTGCTTAGTGTTGTGCAGGAGAAGGGAGTCAAGGCCACTCTGTTAGGGCTGTTCATGAGCTTCATCAAGCTGGTTCCTGGAGTGAAGAAGTACATAGACGCGGAGAAGCAAAAGGTGGTGGATAAATTACAGTCTGGTGGTAACTCTAAGAGAGAAAATTGGAGAACTGAATTGCCTGGCGCAGGGTTGGGACCGGGAGTTCTGGAACAAATGAAAGACGAGAAAACAAAAGATGTGGCTTGGCAAGGCAAATGCTCGGGTACAGTATATATTGGAGGAAGTGAATTCGAAGGACATTTTTCTCTAATTAATGAGGCATGCTCAATGTTTGCACATACCAATCCATTACATATGGATGTATTCCAGAGTGTTGTACGGTTTGAAGCAGAAGTGGTTGCTATGACAGCTGCAATGCTTGGAAGTAAAAAAAAGTCTTCTGGAGGACAAATATGTGGAAACATGACATCAGGAGGAACTGAAAGTATATTACTAGCTGTGAAAACATCACGTGATTATATGAAGGCCAGGAAGGGGATTAAACAACCTGAAATGATAATACCTGAATCTGCTCACTCAGCATACGACAAGGCTGCTCAATATTTTAACATCAAGCTGTGGCGTGTCCCAGTAGATAAAGAGTTCCAAGCAGATGTCAAAGCCATTAGAAGATTCATTAATAGAAACACTGTTTTGATTGTTGGATCCGCACCTGGTTTTCCTCATGGCATTGTTGATCCTATTGAGGAGCTTGGTCAATTAGCTTCTAGCTTTGATATCTGTCTCCATGTCGACCTTTGTCTTGGGGGATTTGTACTACCTTTTGCTCAAAAGCTTGGGTATCCGATTCCACCCTTTGATTTTTCAGTTAAAGGAGTGACATCAATATCAGCTGATGTGCATAAATATGGGTTGGCTCCTAAAGGAACAAGCATAGTTCTGTACAGAAACCATGGCATTAGAAAGCATCAATTTGTAGCTGTTACGGAGTGGTCAGGAGGGTTGTATGTATCTCCAACAATGGCTGGAAGCAGACCTGGTAGCTTAATTGCGGGGGCTTGGGCAGCTATGATGGCTTTGGGTGAGGAAGGGTATTTGGAAAACACAAGGTCTATCATGGAAGTATCCAAAAAACTTCAGAAGGGGTATTATTTTCACCTGTCTTATTTGTTGAGTTATTTGTCTTTGATTTGCCGATGGGGAGCAGGGGTCTTGTTCCATTTGAAAATATGGCCGCTTTGTTGCAGGATAAAGGATATTCCCGAGTTGTTCATCATTGGACGGCCAGACATGACAGTTGTTGCATTTGGCTCTAACGTTATTGATATATTTGAAGTCAATGATATCATGTCATCTAAAGGCTGGCATTTGAATGCATTGCAAAGACCTAACAGGTAG

Protein Analysis

509

Amino Acids

55.7

Weight (kDa)

9.09

Isoelectric Point (pI)

32.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyridoxal_deC PF00282 146 - 425 7.1e-19 Pyridoxal-dependent decarboxylase conserved domain
Aminotran_5 PF00266 200 - 364 1.1e-07 Aminotransferase class-V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011402)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27980
fragaria_vesca FvH4_4g23470 FvH4_4g23470 FvH4_4g23470 FvH4_4g23470 FvH4_4g23470 FvH4_4g23470 FvH4_4g23470
malus_domestica MD13G1122300.v1.1
prunus_persica Prupe.1G222900_v2.0.a1
pyrus_communis pycom13g10640
rosa_chinensis RchiOBHm_Chr4g0429841
rosa_laevigata RLG00000007039
rosa_multiflora Rmu_sc0006762.1_g000006
rosa_roxburghii Rroxscaffold_5G00371600
rosa_rugosa Rorug04G0238500
rosa_samantha Rh4AG294900 Rh4BG301300 Rh4DG298100
rosa_wichuraiana Rw4G025510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 122, 924
AccII CGCG 1 cut(s) 236
AciI CCGC 4 cut(s) 236, 840, 1184, 1367
AclI AACGTT 1 cut(s) 1449
AclWI GGATC 3 cut(s) 831, 844, 863
AcoI YGGCCR 3 cut(s) 63, 1364, 1415
AcsI RAATTY 2 cut(s) 52, 419
AcuI CTGAAG 1 cut(s) 1253
AcvI CACGTG 1 cut(s) 644
AcyI GRCGYC 1 cut(s) 95
AfaI GTAC 5 cut(s) 227, 397, 510, 948, 1076
AfiI CCNNNNNNNGG 3 cut(s) 104, 317, 666
AgsI TTSAA 5 cut(s) 131, 518, 1357, 1466, 1504
AjnI CCWGG 5 cut(s) 211, 310, 659, 844, 1168
AjuI GAANNNNNNNTTGG 2 cut(s) 44, 76
AloI GAACNNNNNNTCC 2 cut(s) 318, 350
Alw26I GTCTC 1 cut(s) 920
AlwI GGATC 3 cut(s) 831, 844, 863
Ama87I CYCGRG 3 cut(s) 39, 391, 1394
AoxI GGCC 5 cut(s) 63, 168, 657, 1364, 1415
ApeKI GCWGC 3 cut(s) 542, 725, 1196
ApoI RAATTY 2 cut(s) 52, 419
AseI ATTAAT 2 cut(s) 444, 810
Asp700I GAANNNNTTC 1 cut(s) 23
AspLEI GCGC 2 cut(s) 38, 317
AspS9I GGNCC 1 cut(s) 326
AsuC2I CCSGG 1 cut(s) 330
AsuHPI GGTGA 3 cut(s) 28, 1226, 1280
AsuII TTCGAA 1 cut(s) 423
AvaI CYCGRG 3 cut(s) 39, 391, 1394
AvaII GGWCC 1 cut(s) 326
BamHI GGATCC 1 cut(s) 836
BbrPI CACGTG 1 cut(s) 644
BbsI GAAGAC 1 cut(s) 561
BbvI GCAGC 3 cut(s) 529, 712, 1208
BccI CCATC 2 cut(s) 1198, 1323
BceAI ACGGC 1 cut(s) 1430
BcgI CGANNNNNNTGC 2 cut(s) 707, 741
BciT130I CCWGG 5 cut(s) 213, 312, 661, 846, 1170
BciVI GTATCC 2 cut(s) 984, 1267
BcnI CCSGG 1 cut(s) 330
BcoDI GTCTC 1 cut(s) 920
BfaI CTAG 2 cut(s) 626, 900
BfoI RGCGCY 1 cut(s) 39
BfuI GTATCC 2 cut(s) 984, 1267
BisI GCNGC 4 cut(s) 543, 726, 1197, 1367
BlsI GCNGC 4 cut(s) 544, 727, 1198, 1368
Bme1390I CCNGG 6 cut(s) 213, 312, 330, 661, 846, 1170
Bme18I GGWCC 1 cut(s) 326
BmeT110I CYCGRG 3 cut(s) 39, 391, 1394
BmgT120I GGNCC 1 cut(s) 326
BmiI GGNNCC 4 cut(s) 210, 327, 838, 1050
BmrFI CCNGG 6 cut(s) 213, 312, 330, 661, 846, 1170
BmrI ACTGGG 1 cut(s) 755
BmsI GCATC 1 cut(s) 1108
BmuI ACTGGG 1 cut(s) 755
BoxI GACNNNNGTC 1 cut(s) 930
BpiI GAAGAC 1 cut(s) 561
BplI GAGNNNNNCTC 2 cut(s) 440, 472
BpmI CTGGAG 2 cut(s) 234, 594
Bpu14I TTCGAA 1 cut(s) 423
BpuMI CCSGG 1 cut(s) 330
BsaAI YACGTR 1 cut(s) 644
BsaHI GRCGYC 1 cut(s) 95
BsaJI CCNNGG 1 cut(s) 1084
BsaXI ACNNNNNCTCC 2 cut(s) 1326, 1356
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 317, 666
Bse118I RCCGGY 1 cut(s) 65
Bse1I ACTGG 1 cut(s) 761
Bse3DI GCAATG 2 cut(s) 552, 1508
BseBI CCWGG 5 cut(s) 213, 312, 661, 846, 1170
BseDI CCNNGG 1 cut(s) 1084
BseGI GGATG 1 cut(s) 496
BseLI CCNNNNNNNGG 3 cut(s) 104, 317, 666
BseMI GCAATG 2 cut(s) 552, 1508
BseMII CTCAG 1 cut(s) 723
BseNI ACTGG 1 cut(s) 761
BseRI GAGGAG 1 cut(s) 893
BseXI GCAGC 3 cut(s) 529, 712, 1208
BsgI GTGCAG 1 cut(s) 170
Bsh1236I CGCG 1 cut(s) 236
BshFI GGCC 5 cut(s) 65, 170, 659, 1366, 1417
BsiHKCI CYCGRG 3 cut(s) 39, 391, 1394
BsiSI CCGG 2 cut(s) 66, 329
BslFI GGGAC 2 cut(s) 339, 743
BslI CCNNNNNNNGG 3 cut(s) 104, 317, 666
BsmAI GTCTC 1 cut(s) 920
BsmFI GGGAC 2 cut(s) 339, 743
BsmI GAATGC 1 cut(s) 1510
BsnI GGCC 5 cut(s) 65, 170, 659, 1366, 1417
BsoBI CYCGRG 3 cut(s) 39, 391, 1394
Bsp119I TTCGAA 1 cut(s) 423
Bsp1407I TGTACA 1 cut(s) 1074
Bsp143I GATC 2 cut(s) 836, 868
Bsp19I CCATGG 1 cut(s) 1084
BspACI CCGC 4 cut(s) 236, 840, 1184, 1367
BspANI GGCC 5 cut(s) 65, 170, 659, 1366, 1417
BspCNI CTCAG 1 cut(s) 722
BspFNI CGCG 1 cut(s) 236
BspHI TCATGA 1 cut(s) 189
BspLI GGNNCC 4 cut(s) 210, 327, 838, 1050
BspPI GGATC 3 cut(s) 831, 844, 863
BspT104I TTCGAA 1 cut(s) 423
BsrDI GCAATG 2 cut(s) 552, 1508
BsrFI RCCGGY 1 cut(s) 65
BsrGI TGTACA 1 cut(s) 1074
BsrI ACTGG 1 cut(s) 761
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 1 cut(s) 1084
BssMI GATC 2 cut(s) 836, 868
BssNI GRCGYC 1 cut(s) 95
BssT1I CCWWGG 1 cut(s) 1084
Bst2UI CCWGG 5 cut(s) 213, 312, 661, 846, 1170
Bst4CI ACNGT 5 cut(s) 267, 400, 513, 823, 1429
Bst6I CTCTTC 1 cut(s) 116
BstACI GRCGYC 1 cut(s) 95
BstAUI TGTACA 1 cut(s) 1074
BstBAI YACGTR 1 cut(s) 644
BstBI TTCGAA 1 cut(s) 423
BstC8I GCNNGC 2 cut(s) 454, 1496
BstDEI CTNAG 3 cut(s) 140, 282, 709
BstDSI CCRYGG 1 cut(s) 1084
BstENI CCTNNNNNAGG 1 cut(s) 315
BstF5I GGATG 1 cut(s) 496
BstFNI CGCG 1 cut(s) 236
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 2 cut(s) 38, 317
BstKTI GATC 2 cut(s) 839, 871
BstMAI GTCTC 1 cut(s) 920
BstMBI GATC 2 cut(s) 836, 868
BstMWI GCNNNNNNNGC 4 cut(s) 539, 710, 1196, 1205
BstNI CCWGG 5 cut(s) 213, 312, 661, 846, 1170
BstNSI RCATGY 1 cut(s) 456
BstPAI GACNNNNGTC 1 cut(s) 930
BstSCI CCNGG 6 cut(s) 211, 310, 328, 659, 844, 1168
BstUI CGCG 1 cut(s) 236
BstV1I GCAGC 3 cut(s) 529, 712, 1208
BstV2I GAAGAC 1 cut(s) 561
BstX2I RGATCY 1 cut(s) 836
BstYI RGATCY 1 cut(s) 836
BsuI GTATCC 2 cut(s) 984, 1267
BsuRI GGCC 5 cut(s) 65, 170, 659, 1366, 1417
BtgI CCRYGG 1 cut(s) 1084
BtsCI GGATG 1 cut(s) 496
BtsIMutI CAGTG 1 cut(s) 819
Cac8I GCNNGC 2 cut(s) 454, 1496
CciI TCATGA 1 cut(s) 189
CfoI GCGC 2 cut(s) 38, 317
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 1 cut(s) 326
CseI GACGC 3 cut(s) 103, 108, 242
CsiI ACCWGGT 2 cut(s) 844, 1168
Csp6I GTAC 5 cut(s) 226, 396, 509, 947, 1075
CspCI CAANNNNNGTGG 2 cut(s) 351, 386
CviAII CATG 9 cut(s) 190, 453, 595, 857, 920, 1085, 1249, 1423, 1480
CviQI GTAC 5 cut(s) 226, 396, 509, 947, 1075
DdeI CTNAG 3 cut(s) 140, 282, 709
DpnI GATC 2 cut(s) 838, 870
DpnII GATC 2 cut(s) 836, 868
EaeI YGGCCR 3 cut(s) 63, 1364, 1415
Eam1104I CTCTTC 1 cut(s) 116
EarI CTCTTC 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 1084
Eco32I GATATC 2 cut(s) 910, 1477
Eco47I GGWCC 1 cut(s) 326
Eco57I CTGAAG 1 cut(s) 1253
Eco72I CACGTG 1 cut(s) 644
Eco88I CYCGRG 3 cut(s) 39, 391, 1394
EcoNI CCTNNNNNAGG 1 cut(s) 315
EcoRI GAATTC 2 cut(s) 52, 419
EcoRII CCWGG 5 cut(s) 211, 310, 659, 844, 1168
EcoRV GATATC 2 cut(s) 910, 1477
EcoT14I CCWWGG 1 cut(s) 1084
EcoT22I ATGCAT 1 cut(s) 1510
ErhI CCWWGG 1 cut(s) 1084
FaeI CATG 9 cut(s) 193, 456, 598, 860, 923, 1088, 1252, 1426, 1483
FaqI GGGAC 2 cut(s) 339, 743
FatI CATG 9 cut(s) 189, 452, 594, 856, 919, 1084, 1248, 1422, 1479
FauI CCCGC 1 cut(s) 1177
FauNDI CATATG 1 cut(s) 486
FblI GTMKAC 2 cut(s) 122, 924
Fnu4HI GCNGC 4 cut(s) 543, 726, 1197, 1367
FokI GGATG 1 cut(s) 503
Fsp4HI GCNGC 4 cut(s) 543, 726, 1197, 1367
FspBI CTAG 2 cut(s) 626, 900
GlaI GCGC 2 cut(s) 37, 316
GluI GCNGC 4 cut(s) 543, 726, 1197, 1367
GsuI CTGGAG 2 cut(s) 234, 594
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 5 cut(s) 65, 170, 659, 1366, 1417
HapII CCGG 2 cut(s) 66, 329
HgaI GACGC 3 cut(s) 103, 108, 242
HhaI GCGC 2 cut(s) 38, 317
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 9 cut(s) 193, 456, 598, 860, 923, 1088, 1252, 1426, 1483
Hin6I GCGC 2 cut(s) 36, 315
HinP1I GCGC 2 cut(s) 36, 315
HincII GTYRAC 2 cut(s) 123, 925
HindII GTYRAC 2 cut(s) 123, 925
HindIII AAGCTT 1 cut(s) 965
HinfI GANTC 6 cut(s) 5, 108, 162, 698, 804, 979
HpaII CCGG 2 cut(s) 66, 329
HphI GGTGA 3 cut(s) 28, 1226, 1280
Hpy166II GTNNAC 2 cut(s) 123, 925
Hpy188I TCNGA 3 cut(s) 102, 978, 1272
Hpy188III TCNNGA 7 cut(s) 190, 338, 499, 573, 603, 1128, 1394
Hpy8I GTNNAC 2 cut(s) 123, 925
Hpy99I CGWCG 3 cut(s) 16, 97, 124
HpyAV CCTTC 6 cut(s) 151, 419, 649, 658, 1214, 1267
HpyCH4III ACNGT 5 cut(s) 267, 400, 513, 823, 1429
HpyCH4IV ACGT 2 cut(s) 643, 1449
HpyCH4V TGCA 8 cut(s) 151, 467, 545, 1033, 1377, 1436, 1508, 1513
HpyF10VI GCNNNNNNNGC 4 cut(s) 539, 710, 1196, 1205
HpyF3I CTNAG 3 cut(s) 140, 282, 709
HpySE526I ACGT 2 cut(s) 643, 1449
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 9 cut(s) 193, 456, 598, 860, 923, 1088, 1252, 1426, 1483
HspAI GCGC 2 cut(s) 36, 315
Kzo9I GATC 2 cut(s) 836, 868
LmnI GCTCC 3 cut(s) 880, 1054, 1334
Lsp1109I GCAGC 3 cut(s) 529, 712, 1208
LweI GCATC 1 cut(s) 1108
MabI ACCWGGT 2 cut(s) 844, 1168
MaeI CTAG 2 cut(s) 626, 900
MaeII ACGT 2 cut(s) 643, 1449
MaeIII GTNAC 4 cut(s) 68, 275, 1009, 1114
MalI GATC 2 cut(s) 838, 870
MboI GATC 2 cut(s) 836, 868
MboII GAAGA 4 cut(s) 103, 232, 561, 813
MflI RGATCY 1 cut(s) 836
MlyI GAGTC 2 cut(s) 102, 171
MmeI TCCRAC 2 cut(s) 814, 1172
Mph1103I ATGCAT 1 cut(s) 1510
MroXI GAANNNNTTC 1 cut(s) 23
MseI TTAA 6 cut(s) 444, 672, 738, 810, 1002, 1178
MslI CAYNNNNRTG 3 cut(s) 489, 1038, 1503
MspA1I CMGCKG 2 cut(s) 542, 1025
MspI CCGG 2 cut(s) 66, 329
MspR9I CCNGG 6 cut(s) 213, 312, 330, 661, 846, 1170
Mva1269I GAATGC 1 cut(s) 1510
MvaI CCWGG 5 cut(s) 213, 312, 661, 846, 1170
MvnI CGCG 1 cut(s) 236
MwoI GCNNNNNNNGC 4 cut(s) 539, 710, 1196, 1205
NciI CCSGG 1 cut(s) 330
NcoI CCATGG 1 cut(s) 1084
NdeI CATATG 1 cut(s) 486
NdeII GATC 2 cut(s) 836, 868
NlaIII CATG 9 cut(s) 193, 456, 598, 860, 923, 1088, 1252, 1426, 1483
NlaIV GGNNCC 4 cut(s) 210, 327, 838, 1050
NmeAIII GCCGAG 1 cut(s) 12
NmuCI GTSAC 1 cut(s) 1009
NsiI ATGCAT 1 cut(s) 1510
NspI RCATGY 1 cut(s) 456
NspV TTCGAA 1 cut(s) 423
PaeI GCATGC 1 cut(s) 456
PaeR7I CTCGAG 1 cut(s) 39
PagI TCATGA 1 cut(s) 189
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 53
PctI GAATGC 1 cut(s) 1510
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 4 cut(s) 5, 698, 804, 979
PfoI TCCNGGA 1 cut(s) 211
PkrI GCNGC 4 cut(s) 544, 727, 1198, 1368
PleI GAGTC 2 cut(s) 102, 170
PmaCI CACGTG 1 cut(s) 644
PmlI CACGTG 1 cut(s) 644
PpsI GAGTC 2 cut(s) 102, 170
Ppu21I YACGTR 1 cut(s) 644
PshAI GACNNNNGTC 1 cut(s) 930
PshBI ATTAAT 2 cut(s) 444, 810
Psp1406I AACGTT 1 cut(s) 1449
Psp6I CCWGG 5 cut(s) 211, 310, 659, 844, 1168
PspCI CACGTG 1 cut(s) 644
PspGI CCWGG 5 cut(s) 211, 310, 659, 844, 1168
PspN4I GGNNCC 4 cut(s) 210, 327, 838, 1050
PspPI GGNCC 1 cut(s) 326
PspXI VCTCGAGB 1 cut(s) 39
PsuI RGATCY 1 cut(s) 836
PvuII CAGCTG 2 cut(s) 542, 1025
RsaI GTAC 5 cut(s) 227, 397, 510, 948, 1076
RsaNI GTAC 5 cut(s) 226, 396, 509, 947, 1075
RseI CAYNNNNRTG 3 cut(s) 489, 1038, 1503
SalI GTCGAC 2 cut(s) 121, 923
SaqAI TTAA 6 cut(s) 444, 672, 738, 810, 1002, 1178
SatI GCNGC 4 cut(s) 543, 726, 1197, 1367
Sau3AI GATC 2 cut(s) 836, 868
Sau96I GGNCC 1 cut(s) 326
SchI GAGTC 2 cut(s) 102, 171
ScrFI CCNGG 6 cut(s) 213, 312, 330, 661, 846, 1170
SexAI ACCWGGT 2 cut(s) 844, 1168
SfaNI GCATC 1 cut(s) 1108
Sfr274I CTCGAG 1 cut(s) 39
SfuI TTCGAA 1 cut(s) 423
SinI GGWCC 1 cut(s) 326
SlaI CTCGAG 1 cut(s) 39
SmiMI CAYNNNNRTG 3 cut(s) 489, 1038, 1503
SmlI CTYRAG 1 cut(s) 39
SmoI CTYRAG 1 cut(s) 39
SphI GCATGC 1 cut(s) 456
SsiI CCGC 4 cut(s) 236, 840, 1184, 1367
SspI AATATT 1 cut(s) 734
SspMI CTAG 2 cut(s) 626, 900
StyD4I CCNGG 6 cut(s) 211, 310, 328, 659, 844, 1168
StyI CCWWGG 1 cut(s) 1084
TaaI ACNGT 5 cut(s) 267, 400, 513, 823, 1429
TaiI ACGT 2 cut(s) 646, 1452
TaqI TCGA 5 cut(s) 40, 92, 122, 423, 924
TatI WGTACW 3 cut(s) 225, 946, 1074
TauI GCSGC 1 cut(s) 1369
TfiI GAWTC 4 cut(s) 5, 698, 804, 979
Tru1I TTAA 6 cut(s) 444, 672, 738, 810, 1002, 1178
Tru9I TTAA 6 cut(s) 444, 672, 738, 810, 1002, 1178
TscAI CASTG 1 cut(s) 826
TseFI GTSAC 1 cut(s) 1009
TseI GCWGC 3 cut(s) 542, 725, 1196
Tsp45I GTSAC 1 cut(s) 1009
TspDTI ATGAA 6 cut(s) 178, 187, 362, 668, 796, 1393
TspGWI ACGGA 1 cut(s) 1133
TspRI CASTG 1 cut(s) 826
VpaK11BI GGWCC 1 cut(s) 326
VspI ATTAAT 2 cut(s) 444, 810
XagI CCTNNNNNAGG 1 cut(s) 315
XapI RAATTY 2 cut(s) 52, 419
XceI RCATGY 1 cut(s) 456
XhoI CTCGAG 1 cut(s) 39
XmiI GTMKAC 2 cut(s) 122, 924
XmnI GAANNNNTTC 1 cut(s) 23
XspI CTAG 2 cut(s) 626, 900
Zsp2I ATGCAT 1 cut(s) 1510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.