Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0017287

Physical Location & Seq

Genomic Coordinates Reverse (-)

26493401 .. 26501238

Gene Structure 7838 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g24260.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2055 bp

ATGGCTGCAAAGAAGAAGACAATGGGTGCAAAGAAGAAGGCCTCGAGCCGTTCTGAGGACTCTGAGGACTCTGAAGAATTAAGGCATGTTCCCCTTGCTGGAGATGCCCTGCGTTTGATATATGAGCGCGATGTGATCCATGGAGATTTTGCCCTCATAAGTGGGGATACTGTAATTGACATGTCCCTTACTCAAGTGCTGCAGGACCATAAAGAGAGAAGAAAGAAGGATAAGAATGCTGTTATGACAATTGTTGTCAAACCTTCTCAAATCGTGACACACCAATCTCGATTACTCGGCAATGATGAGCTGTTTATGGCAATAGATCCTAGCACAAAGCAGCTCTTTTGTTACAAGGATAAGGCAGACCATGCAAAGGAATCTATATTTTGTCTTGATAAGACAATGCTTGCTGGTAAATCTGCGATTTCGCTGCATTATGACAGAGAGGATTGCTACATTGACGTCTGCTCTCCTGAAGTTAGCCTTCTCTCAGATAATTTCGATTACCAAGATCTTCGTCGTGATTTATTGGGTGGATTGCTTGAAGATGACGTTATGGGATACAAGATATTCACACATGAAATTGACTCGAGTAGTTATGCTGCTAAGGTTGACAATTTCCGAAGCTATGATACAATTAGCAAGGACATAATTCAGAGAGGGACTTACCCATCCTCGACACCAGCTGTTCAGGTTAAAGAAATCGGACGTGGCACCAAGATTGGGGACAGTACCGAAATTCTAAGCTCTGTTATTGGGAGAGGGTGTTCTATTGGGTCAAATGTTTCCATAGAAGGTTGTTATATATGGGACAATGTAACCATTGAAGATGGATGCAAGCTAAAACATGTTGTAGTTTGTGATGGAGTGGTCATCAAGTCCGGGACAGTTTTGGAACCTGGAGTAGTTTTGTCTTTCCAGGTTGTTCTAGGAGAACAATGCACTATTCCTTCATATTTAAAGGTATCATTACTTCAGCAACCAACTGAGCAAGATGGTTATGATGAGGAGCTGGAGTATGTCGCCAATAATAGCAATGCAGAAATCTCATCCCCTATTGATTGTGATGTAAATAAACCAAATGAGCTCGGTACTAATGGGGCTCTGGTTGTGTTTGGTCAAAGGGGTTGTTCGAGGGTGGTTGAATCGGTGGCCAGTGGTGCGGCGCCGCTGGCTGGAACATCGATGGGATCAGAGGGAGTAGAATTGGGTGACGAAGGAGTGATGGTGTCTGTGGCTGGCTGCGGGGAAGTTGACTTTTGTGATGAAGATTGGAGAAACTCAATTGCTCCTATTCCTGCAGAGAAACTTGCTGAGGCAATACGTGCCGCTGAGGAGGAGGATCAGGAGTACTTTGCACAAGATGGCAGCAGTCTTGGAGAGCTGGAACTTGAATCTAGTGATTCTGAAGACAACTGTAATGAAGACGATTCTGACTTTGAGAATGAGGTTGAAGCAACCTTCCTAACAGCTGTGCATGAAAATAAAACACTAGATCATGTGGTGTTGGAAGTAAACGCGCTGCGATTGTCATTCAACAAGCAAACTGCAGATTGTGCTGGAGCTATATTTTACTCCGTGATGAAATTGGCATTGGAAGCTCCACATGAATCGGCAAGCGAATTGGTGCGAGCTGCTGCCAAGTCTACCAAGGAATTTGCCTTTGTGTTCGATAAGATATTGGATCAACTCTATGATCAAGAAGTTATCCAAGAAGAAGCCATTCTCAGGTGGGTTGAAGAAAAGACAAATGCAGATGAATCAGATAAAGAGAATATCCAGAGGGTGCTGCACCCAGATGTCTGTCCACGTGTCGACGAGACAGAGGTCACACGTGGATTATCTGATACGTATAGAGTTGGGGTAAATACGGGGGAAAGGGGAAAGCAGGAACCAGAATCGACTAAATGGTGGATTGAGGTTGCCTACCCAAACGGGATCCCAATACACCGCAGGATGAATGTCGACCCTGAACTTGACGATGGTGGTCTTCGATTCAGTGATCAGCAGTTTTCTTCAGATCAAGATGTCTGCCCAGAAGTTGATGGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002181 GO:0002183 GO:0003674 GO:0003676 GO:0003723 GO:0003743 GO:0005085 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005851 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0019538 GO:0019899 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0051020 GO:0065007 GO:0065009 GO:0071704 GO:0097159 GO:0098772 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Hexapep Hexapep_2 NTP_transferase W2

Protein Analysis

685

Amino Acids

75.4

Weight (kDa)

4.48

Isoelectric Point (pI)

45.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LbH_EIF2B	PF25084	236 - 317	6e-30	EIF2B subunit epsilon LbH domain
GMPPB_C	PF25087	237 - 317	3e-07	GMPPB C-terminal domain
W2	PF02020	550 - 592	1.7e-07	eIF4-gamma/eIF5/eIF2-epsilon

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017287)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g24061 FvH4_4g24260 FvH4_6g02711
rosa_chinensis	RchiOBHm_Chr4g0430791
rosa_laevigata	RLG00000006950
rosa_multiflora	Rmu_co8370567.1_g000001 Rmu_sc0002620.1_g000016 Rmu_sc0020793.1_g000001
rosa_roxburghii	Rroxscaffold_5G00372480
rosa_rugosa	Rorug04G0245700
rosa_samantha	Rh4AG301800 Rh4BG309900
rosa_wichuraiana	Rw4G026230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	468
AccB1I	GGYRCC	2 cut(s)	716, 1168
AccI	GTMKAC	3 cut(s)	1649, 1818, 1968
AccII	CGCG	2 cut(s)	129, 1523
AciI	CCGC	5 cut(s)	1166, 1172, 1248, 1332, 1954
AclWI	GGATC	7 cut(s)	130, 320, 1201, 1353, 1695, 1936, 1949
AcoI	YGGCCR	1 cut(s)	1155
AcsI	RAATTY	2 cut(s)	741, 1658
AcuI	CTGAAG	5 cut(s)	93, 498, 962, 1431, 2004
AcvI	CACGTG	2 cut(s)	1814, 1838
AcyI	GRCGYC	2 cut(s)	465, 1169
AfaI	GTAC	3 cut(s)	736, 1096, 1355
AfiI	CCNNNNNNNGG	6 cut(s)	55, 98, 376, 726, 1178, 1731
AflIII	ACRYGT	4 cut(s)	180, 850, 1813, 1835
AgsI	TTSAA	7 cut(s)	548, 830, 1148, 1397, 1457, 1540, 1742
AjiI	CACGTC	1 cut(s)	713
AjnI	CCWGG	2 cut(s)	901, 921
AjuI	GAANNNNNNNTTGG	2 cut(s)	1580, 1612
AloI	GAACNNNNNNTCC	2 cut(s)	754, 786
Alw21I	GWGCWC	1 cut(s)	1092
Alw26I	GTCTC	1 cut(s)	1817
AlwI	GGATC	7 cut(s)	130, 320, 1201, 1353, 1695, 1936, 1949
Ama87I	CYCGRG	2 cut(s)	43, 592
AoxI	GGCC	2 cut(s)	39, 1155
ApoI	RAATTY	2 cut(s)	741, 1658
Asp700I	GAANNNNTTC	1 cut(s)	1725
AspLEI	GCGC	3 cut(s)	129, 1171, 1525
AspS9I	GGNCC	1 cut(s)	205
AsuC2I	CCSGG	1 cut(s)	886
AsuHPI	GGTGA	1 cut(s)	1226
AvaI	CYCGRG	2 cut(s)	43, 592
AvaII	GGWCC	1 cut(s)	205
BalI	TGGCCA	1 cut(s)	1157
BamHI	GGATCC	1 cut(s)	1941
BanI	GGYRCC	2 cut(s)	716, 1168
BanII	GRGCYC	2 cut(s)	1092, 1108
BarI	GAAGNNNNNNTAC	4 cut(s)	619, 651, 960, 992
BbrPI	CACGTG	2 cut(s)	1814, 1838
BbsI	GAAGAC	4 cut(s)	23, 1419, 1434, 1985
Bbv12I	GWGCWC	1 cut(s)	1092
BbvCI	CCTCAGC	2 cut(s)	1317, 1335
BccI	CCATC	9 cut(s)	682, 827, 860, 992, 1183, 1222, 1361, 1979, 2042
BceAI	ACGGC	1 cut(s)	33
BcgI	CGANNNNNNTGC	2 cut(s)	415, 449
BciT130I	CCWGG	2 cut(s)	903, 923
BciVI	GTATCC	2 cut(s)	160, 557
BclI	TGATCA	2 cut(s)	1699, 2005
BcnI	CCSGG	1 cut(s)	886
BcoDI	GTCTC	1 cut(s)	1817
BfaI	CTAG	4 cut(s)	330, 932, 1401, 1496
BfmI	CTRYAG	3 cut(s)	200, 1302, 1551
BfoI	RGCGCY	1 cut(s)	1172
BfuI	GTATCC	2 cut(s)	160, 557
BglII	AGATCT	1 cut(s)	514
BmcAI	AGTACT	1 cut(s)	1355
Bme1390I	CCNGG	3 cut(s)	886, 903, 923
Bme18I	GGWCC	1 cut(s)	205
BmeT110I	CYCGRG	2 cut(s)	43, 592
BmgBI	CACGTC	1 cut(s)	713
BmgT120I	GGNCC	1 cut(s)	205
BmiI	GGNNCC	5 cut(s)	718, 900, 1170, 1896, 1943
BmrFI	CCNGG	3 cut(s)	886, 903, 923
BmsI	GCATC	2 cut(s)	94, 827
BoxI	GACNNNNGTC	1 cut(s)	1829
BpiI	GAAGAC	4 cut(s)	23, 1419, 1434, 1985
BpmI	CTGGAG	4 cut(s)	120, 924, 1037, 1584
Bpu10I	CCTNAGC	3 cut(s)	609, 1317, 1335
BpuEI	CTTGAG	1 cut(s)	177
BpuMI	CCSGG	1 cut(s)	886
Bsa29I	ATCGAT	1 cut(s)	1187
BsaAI	YACGTR	4 cut(s)	1328, 1814, 1838, 1854
BsaHI	GRCGYC	2 cut(s)	465, 1169
BsaJI	CCNNGG	2 cut(s)	139, 1653
BsaXI	ACNNNNNCTCC	6 cut(s)	754, 784, 1004, 1034, 1215, 1245
Bsc4I	CCNNNNNNNGG	6 cut(s)	55, 98, 376, 726, 1178, 1731
Bse1I	ACTGG	1 cut(s)	1158
Bse3DI	GCAATG	2 cut(s)	307, 1045
BseBI	CCWGG	2 cut(s)	903, 923
BseCI	ATCGAT	1 cut(s)	1187
BseDI	CCNNGG	2 cut(s)	139, 1653
BseGI	GGATG	4 cut(s)	674, 842, 1052, 1965
BseLI	CCNNNNNNNGG	6 cut(s)	55, 98, 376, 726, 1178, 1731
BseMI	GCAATG	2 cut(s)	307, 1045
BseMII	CTCAG	7 cut(s)	45, 54, 507, 981, 1308, 1326, 1744
BseNI	ACTGG	1 cut(s)	1158
BseRI	GAGGAG	3 cut(s)	1025, 1352, 1355
BsgI	GTGCAG	1 cut(s)	1778
Bsh1236I	CGCG	2 cut(s)	129, 1523
BshFI	GGCC	2 cut(s)	41, 1157
BshNI	GGYRCC	2 cut(s)	716, 1168
BshVI	ATCGAT	1 cut(s)	1187
BsiHKAI	GWGCWC	1 cut(s)	1092
BsiHKCI	CYCGRG	2 cut(s)	43, 592
BsiSI	CCGG	1 cut(s)	885
BslFI	GGGAC	5 cut(s)	169, 679, 743, 827, 901
BslI	CCNNNNNNNGG	6 cut(s)	55, 98, 376, 726, 1178, 1731
BsmAI	GTCTC	1 cut(s)	1817
BsmFI	GGGAC	5 cut(s)	169, 679, 743, 827, 901
BsmI	GAATGC	1 cut(s)	241
BsnI	GGCC	2 cut(s)	41, 1157
BsoBI	CYCGRG	2 cut(s)	43, 592
Bsp1286I	GDGCHC	2 cut(s)	1092, 1108
Bsp19I	CCATGG	1 cut(s)	139
BspACI	CCGC	5 cut(s)	1166, 1172, 1248, 1332, 1954
BspANI	GGCC	2 cut(s)	41, 1157
BspCNI	CTCAG	7 cut(s)	46, 55, 506, 982, 1309, 1327, 1743
BspDI	ATCGAT	1 cut(s)	1187
BspFNI	CGCG	2 cut(s)	129, 1523
BspLI	GGNNCC	5 cut(s)	718, 900, 1170, 1896, 1943
BspMAI	CTGCAG	3 cut(s)	204, 1306, 1555
BspPI	GGATC	7 cut(s)	130, 320, 1201, 1353, 1695, 1936, 1949
BspT107I	GGYRCC	2 cut(s)	716, 1168
BsrDI	GCAATG	2 cut(s)	307, 1045
BsrI	ACTGG	1 cut(s)	1158
BssECI	CCNNGG	2 cut(s)	139, 1653
BssNI	GRCGYC	2 cut(s)	465, 1169
BssT1I	CCWWGG	2 cut(s)	139, 1653
Bst2UI	CCWGG	2 cut(s)	903, 923
Bst4CI	ACNGT	4 cut(s)	172, 734, 892, 1421
BstACI	GRCGYC	2 cut(s)	465, 1169
BstAPI	GCANNNNNTGC	3 cut(s)	371, 1328, 1559
BstBAI	YACGTR	4 cut(s)	1328, 1814, 1838, 1854
BstC8I	GCNNGC	6 cut(s)	411, 842, 1176, 1243, 1621, 1635
BstDEI	CTNAG	9 cut(s)	54, 63, 493, 609, 746, 990, 1317, 1335, 1730
BstDSI	CCRYGG	1 cut(s)	139
BstF5I	GGATG	4 cut(s)	674, 842, 1052, 1965
BstFNI	CGCG	2 cut(s)	129, 1523
BstH2I	RGCGCY	1 cut(s)	1172
BstHHI	GCGC	3 cut(s)	129, 1171, 1525
BstMAI	GTCTC	1 cut(s)	1817
BstMWI	GCNNNNNNNGC	7 cut(s)	104, 371, 1163, 1175, 1328, 1559, 1601
BstNI	CCWGG	2 cut(s)	903, 923
BstNSI	RCATGY	3 cut(s)	89, 184, 854
BstPAI	GACNNNNGTC	1 cut(s)	1829
BstSCI	CCNGG	3 cut(s)	884, 901, 921
BstSFI	CTRYAG	3 cut(s)	200, 1302, 1551
BstSNI	TACGTA	1 cut(s)	1854
BstUI	CGCG	2 cut(s)	129, 1523
BstV2I	GAAGAC	4 cut(s)	23, 1419, 1434, 1985
BstX2I	RGATCY	3 cut(s)	325, 514, 1941
BstYI	RGATCY	3 cut(s)	325, 514, 1941
Bsu15I	ATCGAT	1 cut(s)	1187
BsuI	GTATCC	2 cut(s)	160, 557
BsuRI	GGCC	2 cut(s)	41, 1157
BsuTUI	ATCGAT	1 cut(s)	1187
BtgI	CCRYGG	1 cut(s)	139
BtgZI	GCGATG	1 cut(s)	144
BtrI	CACGTC	1 cut(s)	713
BtsCI	GGATG	4 cut(s)	674, 842, 1052, 1965
BtsIMutI	CAGTG	2 cut(s)	1165, 2008
Cac8I	GCNNGC	6 cut(s)	411, 842, 1176, 1243, 1621, 1635
CfoI	GCGC	3 cut(s)	129, 1171, 1525
Cfr13I	GGNCC	1 cut(s)	205
ClaI	ATCGAT	1 cut(s)	1187
Csp6I	GTAC	3 cut(s)	735, 1095, 1354
CviAII	CATG	9 cut(s)	86, 140, 181, 371, 581, 851, 1481, 1502, 1610
CviQI	GTAC	3 cut(s)	735, 1095, 1354
DdeI	CTNAG	9 cut(s)	54, 63, 493, 609, 746, 990, 1317, 1335, 1730
DinI	GGCGCC	1 cut(s)	1170
DraI	TTTAAA	1 cut(s)	963
EaeI	YGGCCR	1 cut(s)	1155
Ecl136II	GAGCTC	1 cut(s)	1090
Eco105I	TACGTA	1 cut(s)	1854
Eco130I	CCWWGG	2 cut(s)	139, 1653
Eco147I	AGGCCT	1 cut(s)	41
Eco24I	GRGCYC	2 cut(s)	1092, 1108
Eco47I	GGWCC	1 cut(s)	205
Eco53kI	GAGCTC	1 cut(s)	1090
Eco57I	CTGAAG	5 cut(s)	93, 498, 962, 1431, 2004
Eco72I	CACGTG	2 cut(s)	1814, 1838
Eco88I	CYCGRG	2 cut(s)	43, 592
EcoICRI	GAGCTC	1 cut(s)	1090
EcoRII	CCWGG	2 cut(s)	901, 921
EcoT14I	CCWWGG	2 cut(s)	139, 1653
EcoT38I	GRGCYC	2 cut(s)	1092, 1108
EgeI	GGCGCC	1 cut(s)	1170
EheI	GGCGCC	1 cut(s)	1170
ErhI	CCWWGG	2 cut(s)	139, 1653
FaeI	CATG	9 cut(s)	89, 143, 184, 374, 584, 854, 1484, 1505, 1613
FalI	AAGNNNNNCTT	4 cut(s)	329, 361, 471, 503
FaqI	GGGAC	5 cut(s)	169, 679, 743, 827, 901
FatI	CATG	9 cut(s)	85, 139, 180, 370, 580, 850, 1480, 1501, 1609
FauI	CCCGC	1 cut(s)	1241
FbaI	TGATCA	2 cut(s)	1699, 2005
FblI	GTMKAC	3 cut(s)	1649, 1818, 1968
FokI	GGATG	4 cut(s)	661, 849, 1039, 1972
FriOI	GRGCYC	2 cut(s)	1092, 1108
FspBI	CTAG	4 cut(s)	330, 932, 1401, 1496
GlaI	GCGC	3 cut(s)	128, 1170, 1524
GsuI	CTGGAG	4 cut(s)	120, 924, 1037, 1584
HaeII	RGCGCY	1 cut(s)	1172
HaeIII	GGCC	2 cut(s)	41, 1157
HapII	CCGG	1 cut(s)	885
HhaI	GCGC	3 cut(s)	129, 1171, 1525
Hin1I	GRCGYC	2 cut(s)	465, 1169
Hin1II	CATG	9 cut(s)	89, 143, 184, 374, 584, 854, 1484, 1505, 1613
Hin6I	GCGC	3 cut(s)	127, 1169, 1523
HinP1I	GCGC	3 cut(s)	127, 1169, 1523
HincII	GTYRAC	4 cut(s)	616, 1258, 1819, 1969
HindII	GTYRAC	4 cut(s)	616, 1258, 1819, 1969
HpaII	CCGG	1 cut(s)	885
HphI	GGTGA	1 cut(s)	1226
Hpy166II	GTNNAC	7 cut(s)	616, 1258, 1519, 1650, 1811, 1819, 1969
Hpy188III	TCNNGA	9 cut(s)	274, 288, 395, 476, 524, 1349, 1703, 1783, 2027
Hpy8I	GTNNAC	7 cut(s)	616, 1258, 1519, 1650, 1811, 1819, 1969
Hpy99I	CGWCG	2 cut(s)	525, 1823
HpyAV	CCTTC	8 cut(s)	31, 220, 273, 497, 791, 963, 1214, 1474
HpyCH4III	ACNGT	4 cut(s)	172, 734, 892, 1421
HpyCH4IV	ACGT	7 cut(s)	465, 555, 712, 1327, 1813, 1837, 1853
HpyF10VI	GCNNNNNNNGC	7 cut(s)	104, 371, 1163, 1175, 1328, 1559, 1601
HpyF3I	CTNAG	9 cut(s)	54, 63, 493, 609, 746, 990, 1317, 1335, 1730
HpySE526I	ACGT	7 cut(s)	465, 555, 712, 1327, 1813, 1837, 1853
Hsp92I	GRCGYC	2 cut(s)	465, 1169
Hsp92II	CATG	9 cut(s)	89, 143, 184, 374, 584, 854, 1484, 1505, 1613
HspAI	GCGC	3 cut(s)	127, 1169, 1523
KasI	GGCGCC	1 cut(s)	1168
Ksp22I	TGATCA	2 cut(s)	1699, 2005
LmnI	GCTCC	4 cut(s)	1012, 1297, 1565, 1609
LweI	GCATC	2 cut(s)	94, 827
MaeI	CTAG	4 cut(s)	330, 932, 1401, 1496
MaeII	ACGT	7 cut(s)	465, 555, 712, 1327, 1813, 1837, 1853
MaeIII	GTNAC	5 cut(s)	274, 350, 820, 1214, 1831
MfeI	CAATTG	2 cut(s)	249, 1287
MflI	RGATCY	3 cut(s)	325, 514, 1941
MhlI	GDGCHC	2 cut(s)	1092, 1108
MlsI	TGGCCA	1 cut(s)	1157
MluNI	TGGCCA	1 cut(s)	1157
Mly113I	GGCGCC	1 cut(s)	1169
MlyI	GAGTC	3 cut(s)	53, 62, 584
MmeI	TCCRAC	1 cut(s)	1491
Mox20I	TGGCCA	1 cut(s)	1157
MroXI	GAANNNNTTC	1 cut(s)	1725
MscI	TGGCCA	1 cut(s)	1157
MseI	TTAA	3 cut(s)	80, 699, 962
MslI	CAYNNNNRTG	1 cut(s)	1800
Msp20I	TGGCCA	1 cut(s)	1157
MspA1I	CMGCKG	4 cut(s)	689, 1174, 1334, 1475
MspI	CCGG	1 cut(s)	885
MspR9I	CCNGG	3 cut(s)	886, 903, 923
MunI	CAATTG	2 cut(s)	249, 1287
Mva1269I	GAATGC	1 cut(s)	241
MvaI	CCWGG	2 cut(s)	903, 923
MvnI	CGCG	2 cut(s)	129, 1523
MwoI	GCNNNNNNNGC	7 cut(s)	104, 371, 1163, 1175, 1328, 1559, 1601
NarI	GGCGCC	1 cut(s)	1169
NciI	CCSGG	1 cut(s)	886
NcoI	CCATGG	1 cut(s)	139
NlaIII	CATG	9 cut(s)	89, 143, 184, 374, 584, 854, 1484, 1505, 1613
NlaIV	GGNNCC	5 cut(s)	718, 900, 1170, 1896, 1943
NmeAIII	GCCGAG	1 cut(s)	276
NmuCI	GTSAC	3 cut(s)	274, 1214, 1831
NspI	RCATGY	3 cut(s)	89, 184, 854
PaeR7I	CTCGAG	2 cut(s)	43, 592
PceI	AGGCCT	1 cut(s)	41
PciI	ACATGT	2 cut(s)	180, 850
PctI	GAATGC	1 cut(s)	241
PdmI	GAANNNNTTC	1 cut(s)	1725
PfeI	GAWTC	9 cut(s)	380, 1148, 1397, 1406, 1433, 1613, 1763, 1901, 1998
PfoI	TCCNGGA	1 cut(s)	884
PleI	GAGTC	3 cut(s)	53, 62, 584
PluTI	GGCGCC	1 cut(s)	1172
PmaCI	CACGTG	2 cut(s)	1814, 1838
PmlI	CACGTG	2 cut(s)	1814, 1838
PpsI	GAGTC	3 cut(s)	53, 62, 584
Ppu21I	YACGTR	4 cut(s)	1328, 1814, 1838, 1854
PscI	ACATGT	2 cut(s)	180, 850
PshAI	GACNNNNGTC	1 cut(s)	1829
Psp124BI	GAGCTC	1 cut(s)	1092
Psp6I	CCWGG	2 cut(s)	901, 921
PspCI	CACGTG	2 cut(s)	1814, 1838
PspGI	CCWGG	2 cut(s)	901, 921
PspN4I	GGNNCC	5 cut(s)	718, 900, 1170, 1896, 1943
PspPI	GGNCC	1 cut(s)	205
PspXI	VCTCGAGB	2 cut(s)	43, 592
PstI	CTGCAG	3 cut(s)	204, 1306, 1555
PsuI	RGATCY	3 cut(s)	325, 514, 1941
PvuII	CAGCTG	2 cut(s)	689, 1475
RsaI	GTAC	3 cut(s)	736, 1096, 1355
RsaNI	GTAC	3 cut(s)	735, 1095, 1354
RseI	CAYNNNNRTG	1 cut(s)	1800
SacI	GAGCTC	1 cut(s)	1092
SalI	GTCGAC	2 cut(s)	1817, 1967
SaqAI	TTAA	3 cut(s)	80, 699, 962
Sau96I	GGNCC	1 cut(s)	205
ScaI	AGTACT	1 cut(s)	1355
SchI	GAGTC	3 cut(s)	53, 62, 584
ScrFI	CCNGG	3 cut(s)	886, 903, 923
SduI	GDGCHC	2 cut(s)	1092, 1108
SfaNI	GCATC	2 cut(s)	94, 827
SfcI	CTRYAG	3 cut(s)	200, 1302, 1551
SfoI	GGCGCC	1 cut(s)	1170
Sfr274I	CTCGAG	2 cut(s)	43, 592
SinI	GGWCC	1 cut(s)	205
SlaI	CTCGAG	2 cut(s)	43, 592
SmiMI	CAYNNNNRTG	1 cut(s)	1800
SmlI	CTYRAG	3 cut(s)	43, 192, 592
SmoI	CTYRAG	3 cut(s)	43, 192, 592
SnaBI	TACGTA	1 cut(s)	1854
SseBI	AGGCCT	1 cut(s)	41
SsiI	CCGC	5 cut(s)	1166, 1172, 1248, 1332, 1954
SspDI	GGCGCC	1 cut(s)	1168
SspMI	CTAG	4 cut(s)	330, 932, 1401, 1496
SstI	GAGCTC	1 cut(s)	1092
StuI	AGGCCT	1 cut(s)	41
StyD4I	CCNGG	3 cut(s)	884, 901, 921
StyI	CCWWGG	2 cut(s)	139, 1653
TaaI	ACNGT	4 cut(s)	172, 734, 892, 1421
TaiI	ACGT	7 cut(s)	468, 558, 715, 1330, 1816, 1840, 1856
TatI	WGTACW	1 cut(s)	1353
TauI	GCSGC	3 cut(s)	1169, 1174, 1334
TfiI	GAWTC	9 cut(s)	380, 1148, 1397, 1406, 1433, 1613, 1763, 1901, 1998
Tru1I	TTAA	3 cut(s)	80, 699, 962
Tru9I	TTAA	3 cut(s)	80, 699, 962
TscAI	CASTG	2 cut(s)	1165, 2008
TseFI	GTSAC	3 cut(s)	274, 1214, 1831
Tsp45I	GTSAC	3 cut(s)	274, 1214, 1831
TspDTI	ATGAA	9 cut(s)	597, 945, 1284, 1440, 1497, 1601, 1626, 1776, 1976
TspGWI	ACGGA	1 cut(s)	1570
TspRI	CASTG	2 cut(s)	1165, 2008
VpaK11BI	GGWCC	1 cut(s)	205
XapI	RAATTY	2 cut(s)	741, 1658
XceI	RCATGY	3 cut(s)	89, 184, 854
XhoI	CTCGAG	2 cut(s)	43, 592
XmiI	GTMKAC	3 cut(s)	1649, 1818, 1968
XmnI	GAANNNNTTC	1 cut(s)	1725
XspI	CTAG	4 cut(s)	330, 932, 1401, 1496
ZraI	GACGTC	1 cut(s)	466
ZrmI	AGTACT	1 cut(s)	1355

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.