Rmu_sc0002620.1_g000016

Translation initiation factor eIF-2B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002620.1
OG0017287
Physical Location & Seq
Reverse (-)
66898 .. 73151
6254 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002620.1_g000016.1.cds

Sequence Viewer

Length: 3078 bp
atggttgctagagcattggttatgcgcaccaatctccctgtttctgcttggggctatgcaatattgcatgcagctgtgcttattcgtctgaggcctactgccactcaacccttttctgcatcccagatggttactgggtatgagcctaatgtctcacacttacgcatatttggatgtgcagtttatgtgccaattgcgctgtcactgcgcaccaaaatggtcttcccgtcgttagggggagataagaacaataatgttcaacaggaacgacaggaattgtcgtggtctgtccccactttgtctcgtcttgatccccgaaccgcacagtccgaaattgaagtgcggagaattctcgatcttcagaatgtagcagactctatgcctgacgcgttttctgatatcgctaaagtgacaagatcacatatacctgctgcaaacgtgtctgcaaggattgatgtccctaaaaatagtggacatggcgccacccataggggtattgggtatggcgccaccatcactaatggtgatggcaatgtggctcaggcccggctcccagcaaggaaacgtgggaggcccaaaggttcgatggattctcgcccgagaaagaaagcgagtttggcacaaaaagatccattaatcatcgacataaataacccgtctcataaagatattccggattatggttatgtccaagagacatcattgggggacgctccaatgtcagaaccaattatagagaatagagagatctccatgaattacactagtgtacatgagatgctgagtcgagactctattatccttgatgatgtgtttgcatattctatcgctcaaggaattatagaacatgatgatatcgaacctcgctccgttgaagaatgtcaacgtagaatagactggcctaaatggaaaaatgcgatccaggttgaattggattcactaacaaagagacagcgagggctagctactcggggtctacaaaaggcagaggttcggatcactaagtatttcattgatcgatttctccgaaattttaattcattcatacaaattaagaacgaagagaaggcaatgggtgcaaagaagagggcaatgggtgcaaagaagaaaggtgcatcgaggggttccgaggactctgatgaattgggacaggttcccctgcaggccatcctcttagccgatagttttactaccaaattctaccccgtcacccttgaacgccccaaagtgcttttgccattagtaaatgtccccatgatcagctacaccttagcttggctcaagtctgctggagtcaaggaggtctttgttttctgctgcgctcactccaagcaagtgatcaattatctagagacttctgggtggttttgtcaacctggctgcttcgcagtcaccgcaattgagtcacatgcttctgctagtgctggagatgctctgcgtttgatatacgagcgcgatgtgatccatggagattttgtcctcataagtggggatactgtgagcgacatgtcgcttactcaagtgcttcaagaacataaagagagaagaaagaaagataataatgctgtaatgacaattgttatcaaaccttctcaaatcatgacccaccaatctcgagtacttggcactggtgagctgtttatggcaatagatcctagcacgaagcagcttttgtgttacgaggaaaaggcagaccatgcaaaggaatctatattttgtcttgataagtcgttgcttgttggtaaatcttcgatttctctgcataatgatagagaggattgctatatcgacatctgctctcctgaagtcctcagccttcttgcagacaattttgattacgaagatcttcgtcatgatttattgaatggattgcttgacgatgacgttatgggatacaagatattcacacatgaaattgactctagttatgctgctaagattgacaacttccgaagctacgatgcagttagtaaggacatcattcagagagggagttacccctcgatgccagatgttaagtttacagaaatcggacatggcaccaagattgcggacaatactgaaattttaagttctgttattggggaagggtgttctattgggtcaaatgtttccatagaaggttcttatatattggacaatgtcaccatcgaagatggctgcaagctaaagcatgctgtggtttgtgatggagtggtcatgaagtccggggcaattttggaacctggagtagttttgtctttccaggttgttttaggagaacaatgcactgttccttcatactcaaagatatcgttacttcaacaaccaaccgaacttgcagacgattatagcaatggaattgcagaaatttcgcggcatagtaatagtaatgtaaataaaccaaatgaggttggtactggtggggctggttatgtttggtcaatttgtgatgacgagtggagaaattcagttgctcccattcctgcagacaaacttgctgaggtgatacgtaccacggatggaagcagtctcactccttcaggagaggtaaaacttgaatctagtgaatctgaagacagcgatgaagatacttctgactttgagaatgaggttgaagcaaccttcctaagagccgtgcatgaaaatataaaactagagcatgtagtcttggaagtgaactcgctgagattgtcatccaacaaggaaactgcaatttgtgctggagctatcttttattcgatgatgaaattggcattggaagctccacatgactcggcaagcgaattggttagagctgcttctagggtaatttcagaatggaaacacctgctcaagaactacctatcagacatagatgaggagatagaagtaataatgaagtttgaagaaatgtgtttggagtctgccaaggaattctcctctgtgttcgataagatactgtatcaactgtatgaccaagaagttatccaagaagaagctattctgaggtgggttgaagaaaagacagatgcggatgaatcagacagaattttcgtcaggcaggcggaaacgtttgtccaatggttgagagaggcagaagaggatgatgaagaataa

Protein Analysis

1025

Amino Acids

114.12

Weight (kDa)

5.08

Isoelectric Point (pI)

44.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017287)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24061 FvH4_4g24260 FvH4_6g02711
rosa_chinensis RchiOBHm_Chr4g0430791
rosa_laevigata RLG00000006950
rosa_multiflora Rmu_co8370567.1_g000001 Rmu_sc0002620.1_g000016 Rmu_sc0020793.1_g000001
rosa_roxburghii Rroxscaffold_5G00372480
rosa_rugosa Rorug04G0245700
rosa_samantha Rh4AG301800 Rh4BG309900
rosa_wichuraiana Rw4G026230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2817
Acc16I TGCGCA 2 cut(s) 26, 209
Acc36I ACCTGC 2 cut(s) 436, 2817
AccB1I GGYRCC 3 cut(s) 479, 506, 2039
AccI GTMKAC 1 cut(s) 976
AccII CGCG 3 cut(s) 389, 1455, 2356
AccIII TCCGGA 1 cut(s) 673
AciI CCGC 7 cut(s) 321, 343, 1395, 2051, 2356, 2993, 3026
AclI AACGTT 1 cut(s) 3032
AclWI GGATC 6 cut(s) 305, 623, 913, 1004, 1456, 1646
AcsI RAATTY 8 cut(s) 348, 1030, 1196, 2064, 2349, 2446, 2894, 3009
AcuI CTGAAG 4 cut(s) 344, 1824, 2505, 2574
AcyI GRCGYC 2 cut(s) 480, 507
AfaI GTAC 4 cut(s) 771, 1620, 2398, 2494
AfiI CCNNNNNNNGG 5 cut(s) 233, 489, 680, 1275, 1702
AflIII ACRYGT 3 cut(s) 387, 438, 1506
AhlI ACTAGT 1 cut(s) 764
AjnI CCWGG 4 cut(s) 921, 1375, 2224, 2244
AjuI GAANNNNNNNTTGG 6 cut(s) 206, 238, 599, 631, 2720, 2752
Alw26I GTCTC 8 cut(s) 157, 306, 663, 689, 783, 943, 1346, 2516
AlwI GGATC 6 cut(s) 305, 623, 913, 1004, 1456, 1646
Ama87I CYCGRG 3 cut(s) 598, 969, 1614
Aor13HI TCCGGA 1 cut(s) 673
AoxI GGCC 5 cut(s) 92, 543, 572, 899, 1164
ApeKI GCWGC 8 cut(s) 71, 431, 1317, 1380, 1666, 1931, 2160, 2777
ApoI RAATTY 8 cut(s) 348, 1030, 1196, 2064, 2349, 2446, 2894, 3009
ArsI GACNNNNNNTTYG 2 cut(s) 2996, 3028
AseI ATTAAT 1 cut(s) 635
Asp700I GAANNNNTTC 2 cut(s) 1845, 2961
AspLEI GCGC 7 cut(s) 27, 199, 210, 482, 509, 1322, 1455
AspS9I GGNCC 2 cut(s) 544, 573
AsuC2I CCSGG 2 cut(s) 547, 2209
AsuHPI GGTGA 6 cut(s) 536, 1201, 1384, 1643, 2137, 2497
AsuNHI GCTAGC 1 cut(s) 961
AvaI CYCGRG 3 cut(s) 598, 969, 1614
BanI GGYRCC 3 cut(s) 479, 506, 2039
BbsI GAAGAC 2 cut(s) 214, 2562
BbvCI CCTCAGC 2 cut(s) 1811, 2481
BbvI GCAGC 8 cut(s) 83, 418, 1304, 1367, 1678, 1918, 2147, 2764
BccI CCATC 9 cut(s) 121, 521, 521, 580, 1175, 2150, 2156, 2183, 2495
BceAI ACGGC 1 cut(s) 2600
BcgI CGANNNNNNTGC 4 cut(s) 1741, 1775, 2334, 2368
BciT130I CCWGG 4 cut(s) 923, 1377, 2226, 2246
BciVI GTATCC 2 cut(s) 1486, 1886
BclI TGATCA 2 cut(s) 1257, 1338
BcnI CCSGG 2 cut(s) 547, 2209
BcoDI GTCTC 8 cut(s) 157, 306, 663, 689, 783, 943, 1346, 2516
BcuI ACTAGT 1 cut(s) 764
BfmI CTRYAG 2 cut(s) 1160, 2466
BfoI RGCGCY 2 cut(s) 483, 510
BfuAI ACCTGC 2 cut(s) 436, 2817
BfuI GTATCC 2 cut(s) 1486, 1886
BglII AGATCT 2 cut(s) 747, 1843
BisI GCNGC 9 cut(s) 72, 432, 1318, 1381, 1667, 1932, 2161, 2357, 2778
BlsI GCNGC 9 cut(s) 73, 433, 1319, 1382, 1668, 1933, 2162, 2358, 2779
BmcAI AGTACT 1 cut(s) 1620
Bme1390I CCNGG 6 cut(s) 547, 923, 1377, 2209, 2226, 2246
BmeT110I CYCGRG 3 cut(s) 598, 969, 1614
BmgT120I GGNCC 2 cut(s) 544, 573
BmiI GGNNCC 7 cut(s) 481, 508, 551, 1126, 1155, 2041, 2223
BmrFI CCNGG 6 cut(s) 547, 923, 1377, 2209, 2226, 2246
BmrI ACTGGG 1 cut(s) 144
BmsI GCATC 7 cut(s) 128, 768, 1124, 1420, 1951, 1995, 2980
BmtI GCTAGC 1 cut(s) 965
BmuI ACTGGG 1 cut(s) 144
BpiI GAAGAC 2 cut(s) 214, 2562
BpmI CTGGAG 4 cut(s) 1311, 1446, 2247, 2724
Bpu10I CCTNAGC 4 cut(s) 540, 1270, 1811, 2481
BpuEI CTTGAG 4 cut(s) 816, 1265, 1503, 2798
BpuMI CCSGG 2 cut(s) 547, 2209
Bsa29I ATCGAT 1 cut(s) 1018
BsaAI YACGTR 1 cut(s) 2492
BsaBI GATNNNNATC 1 cut(s) 2674
BsaHI GRCGYC 2 cut(s) 480, 507
BsaJI CCNNGG 5 cut(s) 1128, 1465, 2208, 2496, 2889
BsaWI WCCGGW 1 cut(s) 673
BsaXI ACNNNNNCTCC 2 cut(s) 1461, 1491
Bsc4I CCNNNNNNNGG 5 cut(s) 233, 489, 680, 1275, 1702
Bse1I ACTGG 4 cut(s) 139, 902, 1633, 2404
Bse3DI GCAATG 4 cut(s) 538, 1077, 1098, 2341
Bse8I GATNNNNATC 1 cut(s) 2674
BseAI TCCGGA 1 cut(s) 673
BseBI CCWGG 4 cut(s) 923, 1377, 2226, 2246
BseCI ATCGAT 1 cut(s) 1018
BseDI CCNNGG 5 cut(s) 1128, 1465, 2208, 2496, 2889
BseGI GGATG 7 cut(s) 119, 179, 1167, 2506, 2675, 3001, 3070
BseJI GATNNNNATC 1 cut(s) 2674
BseLI CCNNNNNNNGG 5 cut(s) 233, 489, 680, 1275, 1702
BseMI GCAATG 4 cut(s) 538, 1077, 1098, 2341
BseMII CTCAG 7 cut(s) 80, 554, 773, 1825, 2472, 2657, 2957
BseNI ACTGG 4 cut(s) 139, 902, 1633, 2404
BseRI GAGGAG 2 cut(s) 2855, 2890
BseXI GCAGC 8 cut(s) 83, 418, 1304, 1367, 1678, 1918, 2147, 2764
BseYI CCCAGC 1 cut(s) 553
BsgI GTGCAG 1 cut(s) 198
Bsh1236I CGCG 3 cut(s) 389, 1455, 2356
BshFI GGCC 5 cut(s) 94, 545, 574, 901, 1166
BshNI GGYRCC 3 cut(s) 479, 506, 2039
BshVI ATCGAT 1 cut(s) 1018
BsiHKCI CYCGRG 3 cut(s) 598, 969, 1614
BsiSI CCGG 3 cut(s) 547, 674, 2208
BslFI GGGAC 5 cut(s) 275, 443, 722, 1161, 1235
BslI CCNNNNNNNGG 5 cut(s) 233, 489, 680, 1275, 1702
BsmAI GTCTC 8 cut(s) 157, 306, 663, 689, 783, 943, 1346, 2516
BsmBI CGTCTC 1 cut(s) 663
BsmFI GGGAC 5 cut(s) 275, 443, 722, 1161, 1235
BsnI GGCC 5 cut(s) 94, 545, 574, 901, 1166
BsoBI CYCGRG 3 cut(s) 598, 969, 1614
Bsp13I TCCGGA 1 cut(s) 673
Bsp1407I TGTACA 1 cut(s) 769
Bsp19I CCATGG 1 cut(s) 1465
BspACI CCGC 7 cut(s) 321, 343, 1395, 2051, 2356, 2993, 3026
BspANI GGCC 5 cut(s) 94, 545, 574, 901, 1166
BspCNI CTCAG 7 cut(s) 81, 553, 774, 1824, 2473, 2658, 2958
BspDI ATCGAT 1 cut(s) 1018
BspEI TCCGGA 1 cut(s) 673
BspFNI CGCG 3 cut(s) 389, 1455, 2356
BspHI TCATGA 3 cut(s) 1599, 1852, 2199
BspLI GGNNCC 7 cut(s) 481, 508, 551, 1126, 1155, 2041, 2223
BspMAI CTGCAG 2 cut(s) 1164, 2470
BspMI ACCTGC 2 cut(s) 436, 2817
BspOI GCTAGC 1 cut(s) 965
BspPI GGATC 6 cut(s) 305, 623, 913, 1004, 1456, 1646
BspT107I GGYRCC 3 cut(s) 479, 506, 2039
BsrDI GCAATG 4 cut(s) 538, 1077, 1098, 2341
BsrGI TGTACA 1 cut(s) 769
BsrI ACTGG 4 cut(s) 139, 902, 1633, 2404
BssECI CCNNGG 5 cut(s) 1128, 1465, 2208, 2496, 2889
BssNI GRCGYC 2 cut(s) 480, 507
BssT1I CCWWGG 2 cut(s) 1465, 2889
Bst2UI CCWGG 4 cut(s) 923, 1377, 2226, 2246
Bst4CI ACNGT 5 cut(s) 327, 1498, 2272, 2922, 2931
Bst6I CTCTTC 3 cut(s) 1056, 1079, 3054
BstACI GRCGYC 2 cut(s) 480, 507
BstAPI GCANNNNNTGC 4 cut(s) 1076, 1097, 1697, 2699
BstAUI TGTACA 1 cut(s) 769
BstBAI YACGTR 1 cut(s) 2492
BstC8I GCNNGC 7 cut(s) 69, 963, 1164, 2165, 2175, 2761, 3024
BstDSI CCRYGG 2 cut(s) 1465, 2496
BstF5I GGATG 7 cut(s) 119, 179, 1167, 2506, 2675, 3001, 3070
BstFNI CGCG 3 cut(s) 389, 1455, 2356
BstH2I RGCGCY 2 cut(s) 483, 510
BstHHI GCGC 7 cut(s) 27, 199, 210, 482, 509, 1322, 1455
BstMAI GTCTC 8 cut(s) 157, 306, 663, 689, 783, 943, 1346, 2516
BstNI CCWGG 4 cut(s) 923, 1377, 2226, 2246
BstNSI RCATGY 5 cut(s) 71, 1412, 1510, 2177, 2645
BstSCI CCNGG 6 cut(s) 545, 921, 1375, 2207, 2224, 2244
BstSFI CTRYAG 2 cut(s) 1160, 2466
BstSNI TACGTA 1 cut(s) 2492
BstUI CGCG 3 cut(s) 389, 1455, 2356
BstV1I GCAGC 8 cut(s) 83, 418, 1304, 1367, 1678, 1918, 2147, 2764
BstV2I GAAGAC 2 cut(s) 214, 2562
BstX2I RGATCY 4 cut(s) 628, 747, 1651, 1843
BstYI RGATCY 4 cut(s) 628, 747, 1651, 1843
Bsu15I ATCGAT 1 cut(s) 1018
BsuI GTATCC 2 cut(s) 1486, 1886
BsuRI GGCC 5 cut(s) 94, 545, 574, 901, 1166
BsuTUI ATCGAT 1 cut(s) 1018
BtgI CCRYGG 2 cut(s) 1465, 2496
BtgZI GCGATG 2 cut(s) 1470, 2577
BtsCI GGATG 7 cut(s) 119, 179, 1167, 2506, 2675, 3001, 3070
BtsI GCAGTG 1 cut(s) 203
BtsIMutI CAGTG 3 cut(s) 203, 1626, 2268
BveI ACCTGC 2 cut(s) 436, 2817
Cac8I GCNNGC 7 cut(s) 69, 963, 1164, 2165, 2175, 2761, 3024
CciI TCATGA 3 cut(s) 1599, 1852, 2199
CfoI GCGC 7 cut(s) 27, 199, 210, 482, 509, 1322, 1455
Cfr13I GGNCC 2 cut(s) 544, 573
ClaI ATCGAT 1 cut(s) 1018
CseI GACGC 2 cut(s) 395, 719
Csp6I GTAC 4 cut(s) 770, 1619, 2397, 2493
CviQI GTAC 4 cut(s) 770, 1619, 2397, 2493
DinI GGCGCC 2 cut(s) 481, 508
Eam1104I CTCTTC 3 cut(s) 1056, 1079, 3054
EarI CTCTTC 3 cut(s) 1056, 1079, 3054
EciI GGCGGA 1 cut(s) 3041
Eco105I TACGTA 1 cut(s) 2492
Eco130I CCWWGG 2 cut(s) 1465, 2889
Eco147I AGGCCT 1 cut(s) 94
Eco32I GATATC 3 cut(s) 400, 856, 2292
Eco57I CTGAAG 4 cut(s) 344, 1824, 2505, 2574
Eco88I CYCGRG 3 cut(s) 598, 969, 1614
EcoRI GAATTC 2 cut(s) 348, 2894
EcoRII CCWGG 4 cut(s) 921, 1375, 2224, 2244
EcoRV GATATC 3 cut(s) 400, 856, 2292
EcoT14I CCWWGG 2 cut(s) 1465, 2889
EgeI GGCGCC 2 cut(s) 481, 508
EheI GGCGCC 2 cut(s) 481, 508
ErhI CCWWGG 2 cut(s) 1465, 2889
Esp3I CGTCTC 1 cut(s) 663
FalI AAGNNNNNCTT 2 cut(s) 1289, 1321
FaqI GGGAC 5 cut(s) 275, 443, 722, 1161, 1235
FbaI TGATCA 2 cut(s) 1257, 1338
FblI GTMKAC 1 cut(s) 976
Fnu4HI GCNGC 9 cut(s) 72, 432, 1318, 1381, 1667, 1932, 2161, 2357, 2778
FokI GGATG 6 cut(s) 106, 186, 1154, 2513, 2662, 3008
Fsp4HI GCNGC 9 cut(s) 72, 432, 1318, 1381, 1667, 1932, 2161, 2357, 2778
FspAI RTGCGCAY 1 cut(s) 26
FspI TGCGCA 2 cut(s) 26, 209
GlaI GCGC 7 cut(s) 26, 198, 209, 481, 508, 1321, 1454
GluI GCNGC 9 cut(s) 72, 432, 1318, 1381, 1667, 1932, 2161, 2357, 2778
GsaI CCCAGC 1 cut(s) 557
GsuI CTGGAG 4 cut(s) 1311, 1446, 2247, 2724
HaeII RGCGCY 2 cut(s) 483, 510
HaeIII GGCC 5 cut(s) 94, 545, 574, 901, 1166
HapII CCGG 3 cut(s) 547, 674, 2208
HgaI GACGC 2 cut(s) 395, 719
HhaI GCGC 7 cut(s) 27, 199, 210, 482, 509, 1322, 1455
Hin1I GRCGYC 2 cut(s) 480, 507
Hin6I GCGC 7 cut(s) 25, 197, 208, 480, 507, 1320, 1453
HinP1I GCGC 7 cut(s) 25, 197, 208, 480, 507, 1320, 1453
HincII GTYRAC 2 cut(s) 884, 1373
HindII GTYRAC 2 cut(s) 884, 1373
HpaII CCGG 3 cut(s) 547, 674, 2208
HphI GGTGA 6 cut(s) 536, 1201, 1384, 1643, 2137, 2497
Hpy166II GTNNAC 7 cut(s) 473, 770, 884, 977, 1373, 2022, 2659
Hpy8I GTNNAC 7 cut(s) 473, 770, 884, 977, 1373, 2022, 2659
Hpy99I CGWCG 1 cut(s) 232
HpyAV CCTTC 8 cut(s) 1060, 1599, 1826, 2081, 2114, 2286, 2529, 2614
HpyCH4III ACNGT 5 cut(s) 327, 1498, 2272, 2922, 2931
HpyCH4IV ACGT 6 cut(s) 438, 565, 886, 1884, 2491, 3032
HpySE526I ACGT 6 cut(s) 438, 565, 886, 1884, 2491, 3032
Hsp92I GRCGYC 2 cut(s) 480, 507
HspAI GCGC 7 cut(s) 25, 197, 208, 480, 507, 1320, 1453
KasI GGCGCC 2 cut(s) 479, 506
Kpn2I TCCGGA 1 cut(s) 673
Ksp22I TGATCA 2 cut(s) 1257, 1338
LmnI GCTCC 6 cut(s) 555, 718, 872, 2461, 2705, 2749
Lsp1109I GCAGC 8 cut(s) 83, 418, 1304, 1367, 1678, 1918, 2147, 2764
LweI GCATC 7 cut(s) 128, 768, 1124, 1420, 1951, 1995, 2980
MaeII ACGT 6 cut(s) 438, 565, 886, 1884, 2491, 3032
MfeI CAATTG 3 cut(s) 192, 1398, 1575
MflI RGATCY 4 cut(s) 628, 747, 1651, 1843
MluI ACGCGT 1 cut(s) 387
Mly113I GGCGCC 2 cut(s) 480, 507
MlyI GAGTC 9 cut(s) 368, 785, 793, 1127, 1302, 1412, 1913, 2747, 2891
MmeI TCCRAC 1 cut(s) 2703
MroI TCCGGA 1 cut(s) 673
MroXI GAANNNNTTC 2 cut(s) 1845, 2961
MseI TTAA 5 cut(s) 635, 1035, 1053, 2016, 2069
MslI CAYNNNNRTG 1 cut(s) 215
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 3 cut(s) 547, 674, 2208
MspR9I CCNGG 6 cut(s) 547, 923, 1377, 2209, 2226, 2246
MunI CAATTG 3 cut(s) 192, 1398, 1575
MvaI CCWGG 4 cut(s) 923, 1377, 2226, 2246
MvnI CGCG 3 cut(s) 389, 1455, 2356
NarI GGCGCC 2 cut(s) 480, 507
NciI CCSGG 2 cut(s) 547, 2209
NcoI CCATGG 1 cut(s) 1465
NheI GCTAGC 1 cut(s) 961
NlaIV GGNNCC 7 cut(s) 481, 508, 551, 1126, 1155, 2041, 2223
NmeAIII GCCGAG 1 cut(s) 2735
NmuCI GTSAC 6 cut(s) 201, 409, 1207, 1390, 1404, 2143
NsbI TGCGCA 2 cut(s) 26, 209
NspI RCATGY 5 cut(s) 71, 1412, 1510, 2177, 2645
PaeI GCATGC 2 cut(s) 71, 2177
PaeR7I CTCGAG 1 cut(s) 1614
PagI TCATGA 3 cut(s) 1599, 1852, 2199
PaqCI CACCTGC 1 cut(s) 2817
PceI AGGCCT 1 cut(s) 94
PciI ACATGT 1 cut(s) 1506
PcsI WCGNNNNNNNCGW 1 cut(s) 1024
PdmI GAANNNNTTC 2 cut(s) 1845, 2961
PfeI GAWTC 6 cut(s) 590, 935, 1706, 2540, 2549, 2999
PflFI GACNNNGTC 1 cut(s) 2141
PkrI GCNGC 9 cut(s) 73, 433, 1319, 1382, 1668, 1933, 2162, 2358, 2779
PleI GAGTC 9 cut(s) 368, 785, 792, 1127, 1301, 1411, 1913, 2747, 2890
PluTI GGCGCC 2 cut(s) 483, 510
PpsI GAGTC 9 cut(s) 368, 785, 792, 1127, 1301, 1411, 1913, 2747, 2890
Ppu21I YACGTR 1 cut(s) 2492
PscI ACATGT 1 cut(s) 1506
PshBI ATTAAT 1 cut(s) 635
Psp1406I AACGTT 1 cut(s) 3032
Psp6I CCWGG 4 cut(s) 921, 1375, 2224, 2244
PspFI CCCAGC 1 cut(s) 553
PspGI CCWGG 4 cut(s) 921, 1375, 2224, 2244
PspN4I GGNNCC 7 cut(s) 481, 508, 551, 1126, 1155, 2041, 2223
PspPI GGNCC 2 cut(s) 544, 573
PstI CTGCAG 2 cut(s) 1164, 2470
PsuI RGATCY 4 cut(s) 628, 747, 1651, 1843
PsyI GACNNNGTC 1 cut(s) 2141
PvuII CAGCTG 1 cut(s) 74
RsaI GTAC 4 cut(s) 771, 1620, 2398, 2494
RsaNI GTAC 4 cut(s) 770, 1619, 2397, 2493
RseI CAYNNNNRTG 1 cut(s) 215
SaqAI TTAA 5 cut(s) 635, 1035, 1053, 2016, 2069
SatI GCNGC 9 cut(s) 72, 432, 1318, 1381, 1667, 1932, 2161, 2357, 2778
Sau96I GGNCC 2 cut(s) 544, 573
SbfI CCTGCAGG 1 cut(s) 1164
ScaI AGTACT 1 cut(s) 1620
SchI GAGTC 9 cut(s) 368, 785, 793, 1127, 1302, 1412, 1913, 2747, 2891
ScrFI CCNGG 6 cut(s) 547, 923, 1377, 2209, 2226, 2246
SdaI CCTGCAGG 1 cut(s) 1164
SfaNI GCATC 7 cut(s) 128, 768, 1124, 1420, 1951, 1995, 2980
SfcI CTRYAG 2 cut(s) 1160, 2466
SfoI GGCGCC 2 cut(s) 481, 508
Sfr274I CTCGAG 1 cut(s) 1614
SlaI CTCGAG 1 cut(s) 1614
SmiMI CAYNNNNRTG 1 cut(s) 215
SmlI CTYRAG 5 cut(s) 831, 1280, 1518, 1614, 2813
SmoI CTYRAG 5 cut(s) 831, 1280, 1518, 1614, 2813
SnaBI TACGTA 1 cut(s) 2492
SpeI ACTAGT 1 cut(s) 764
SphI GCATGC 2 cut(s) 71, 2177
Sse8387I CCTGCAGG 1 cut(s) 1164
SseBI AGGCCT 1 cut(s) 94
SsiI CCGC 7 cut(s) 321, 343, 1395, 2051, 2356, 2993, 3026
SspDI GGCGCC 2 cut(s) 479, 506
SspI AATATT 1 cut(s) 63
StuI AGGCCT 1 cut(s) 94
StyD4I CCNGG 6 cut(s) 545, 921, 1375, 2207, 2224, 2244
StyI CCWWGG 2 cut(s) 1465, 2889
TaaI ACNGT 5 cut(s) 327, 1498, 2272, 2922, 2931
TaiI ACGT 6 cut(s) 441, 568, 889, 1887, 2494, 3035
TatI WGTACW 2 cut(s) 769, 1618
TauI GCSGC 1 cut(s) 2359
TfiI GAWTC 6 cut(s) 590, 935, 1706, 2540, 2549, 2999
Tru1I TTAA 5 cut(s) 635, 1035, 1053, 2016, 2069
Tru9I TTAA 5 cut(s) 635, 1035, 1053, 2016, 2069
TscAI CASTG 3 cut(s) 210, 1633, 2275
TseFI GTSAC 6 cut(s) 201, 409, 1207, 1390, 1404, 2143
TseI GCWGC 8 cut(s) 71, 431, 1317, 1380, 1666, 1931, 2160, 2777
Tsp45I GTSAC 6 cut(s) 201, 409, 1207, 1390, 1404, 2143
TspGWI ACGGA 2 cut(s) 859, 2513
TspRI CASTG 3 cut(s) 210, 1633, 2275
Tth111I GACNNNGTC 1 cut(s) 2141
VspI ATTAAT 1 cut(s) 635
XapI RAATTY 8 cut(s) 348, 1030, 1196, 2064, 2349, 2446, 2894, 3009
XbaI TCTAGA 1 cut(s) 1348
XceI RCATGY 5 cut(s) 71, 1412, 1510, 2177, 2645
XcmI CCANNNNNNNNNTGG 3 cut(s) 131, 494, 583
XhoI CTCGAG 1 cut(s) 1614
XmiI GTMKAC 1 cut(s) 976
XmnI GAANNNNTTC 2 cut(s) 1845, 2961
ZrmI AGTACT 1 cut(s) 1620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.