FvH4_4g24940

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015605
Physical Location & Seq
Reverse (-)
26886526 .. 26887315
790 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24940.t1

Sequence Viewer

Length: 543 bp
ATGAAGAGTCTAACATCCCTAGCAGCAGTTCTCATATGTTTCTTTCAAATTTCTCTTCTTCCAATATGCCATTGCCGCAGTGCTTTTCCTTCGATGGATGTCAATCTCATCAAGGAAACATGCAGCAAAACACCTAAACCCGCCCTCTGTGTCGAGTCTATTACCAAGGTCCCTCAAAGCGCCCGTGCAAACGTCAAAGGCTTAGCACAGATAATGAACGACGACGTACTCAGGGCTAAAGCACAAGAAGTTCACACCCAGATCGATCTGCTTATTAAGCAAGGCACCAGACCACAGTCATTACTCAAATGTGCACAGGATTACAGCGTTGTTATCAATGATAAAATCAAAGGAGTCGATGAAGGATTGAAGGATAATCAGACTTCGAAGGCGGCACAAGCCATGGATGATACTGTGTCTTTGGTCAGTGGCTGTAACAGTGGCTTGAAGCAATCTCTGCCCGGTACTCCTCTGATTGGCATGAACACAAATGTAGCGGATCTTGCGACTGTGACCAAAGCTATAGTGCAGCAATTGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

181

Amino Acids

19.27

Weight (kDa)

8.69

Isoelectric Point (pI)

26.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 35 - 175 2.4e-12 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 284
AciI CCGC 4 cut(s) 76, 141, 392, 497
AclWI GGATC 1 cut(s) 507
AcsI RAATTY 1 cut(s) 48
AfaI GTAC 2 cut(s) 228, 466
AfiI CCNNNNNNNGG 1 cut(s) 476
AgsI TTSAA 3 cut(s) 47, 370, 448
AluBI AGCT 1 cut(s) 521
AluI AGCT 1 cut(s) 521
Alw21I GWGCWC 1 cut(s) 316
Alw44I GTGCAC 1 cut(s) 312
AlwI GGATC 1 cut(s) 507
AlwNI CAGNNNCTG 1 cut(s) 432
ApaLI GTGCAC 1 cut(s) 312
ApeKI GCWGC 3 cut(s) 23, 123, 529
ApoI RAATTY 1 cut(s) 48
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 1 cut(s) 169
AsuC2I CCSGG 1 cut(s) 462
AsuII TTCGAA 1 cut(s) 386
AvaII GGWCC 1 cut(s) 169
BaeGI GKGCMC 1 cut(s) 316
BanI GGYRCC 1 cut(s) 284
Bbv12I GWGCWC 1 cut(s) 316
BbvI GCAGC 2 cut(s) 35, 135
BccI CCATC 1 cut(s) 88
BcnI CCSGG 1 cut(s) 462
BfaI CTAG 1 cut(s) 20
BfmI CTRYAG 1 cut(s) 522
BfoI RGCGCY 1 cut(s) 183
BisI GCNGC 5 cut(s) 24, 76, 124, 393, 530
BlpI GCTNAGC 1 cut(s) 202
BlsI GCNGC 5 cut(s) 25, 77, 125, 394, 531
Bme1390I CCNGG 1 cut(s) 462
Bme18I GGWCC 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 169
BmiI GGNNCC 2 cut(s) 171, 286
BmrFI CCNGG 1 cut(s) 462
BoxI GACNNNNGTC 1 cut(s) 295
Bpu1102I GCTNAGC 1 cut(s) 202
Bpu14I TTCGAA 1 cut(s) 386
BpuMI CCSGG 1 cut(s) 462
Bsa29I ATCGAT 1 cut(s) 264
BsaBI GATNNNNATC 1 cut(s) 102
BsaJI CCNNGG 2 cut(s) 165, 402
Bsc4I CCNNNNNNNGG 1 cut(s) 476
Bse3DI GCAATG 1 cut(s) 70
Bse8I GATNNNNATC 1 cut(s) 102
BseCI ATCGAT 1 cut(s) 264
BseDI CCNNGG 2 cut(s) 165, 402
BseGI GGATG 3 cut(s) 14, 103, 412
BseJI GATNNNNATC 1 cut(s) 102
BseLI CCNNNNNNNGG 1 cut(s) 476
BseMI GCAATG 1 cut(s) 70
BseMII CTCAG 1 cut(s) 244
BseRI GAGGAG 1 cut(s) 459
BseSI GKGCMC 1 cut(s) 316
BseXI GCAGC 2 cut(s) 35, 135
BshNI GGYRCC 1 cut(s) 284
BshVI ATCGAT 1 cut(s) 264
BsiHKAI GWGCWC 1 cut(s) 316
BsiSI CCGG 1 cut(s) 462
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 1 cut(s) 476
BsmFI GGGAC 1 cut(s) 155
Bsp119I TTCGAA 1 cut(s) 386
Bsp1286I GDGCHC 1 cut(s) 316
Bsp143I GATC 3 cut(s) 261, 265, 499
Bsp1720I GCTNAGC 1 cut(s) 202
Bsp19I CCATGG 1 cut(s) 402
BspACI CCGC 4 cut(s) 76, 141, 392, 497
BspCNI CTCAG 1 cut(s) 243
BspDI ATCGAT 1 cut(s) 264
BspLI GGNNCC 2 cut(s) 171, 286
BspPI GGATC 1 cut(s) 507
BspT104I TTCGAA 1 cut(s) 386
BspT107I GGYRCC 1 cut(s) 284
BsrDI GCAATG 1 cut(s) 70
BssECI CCNNGG 2 cut(s) 165, 402
BssMI GATC 3 cut(s) 261, 265, 499
BssT1I CCWWGG 2 cut(s) 165, 402
Bst4CI ACNGT 4 cut(s) 297, 415, 440, 511
Bst6I CTCTTC 1 cut(s) 60
BstAPI GCANNNNNTGC 1 cut(s) 457
BstBI TTCGAA 1 cut(s) 386
BstDEI CTNAG 2 cut(s) 202, 230
BstDSI CCRYGG 1 cut(s) 402
BstF5I GGATG 3 cut(s) 14, 103, 412
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 3 cut(s) 264, 268, 502
BstMBI GATC 3 cut(s) 261, 265, 499
BstMWI GCNNNNNNNGC 5 cut(s) 75, 277, 398, 457, 503
BstNSI RCATGY 1 cut(s) 123
BstPAI GACNNNNGTC 1 cut(s) 295
BstSCI CCNGG 1 cut(s) 460
BstSFI CTRYAG 1 cut(s) 522
BstSLI GKGCMC 1 cut(s) 316
BstV1I GCAGC 2 cut(s) 35, 135
BstX2I RGATCY 1 cut(s) 499
BstYI RGATCY 1 cut(s) 499
Bsu15I ATCGAT 1 cut(s) 264
BsuTUI ATCGAT 1 cut(s) 264
BtgI CCRYGG 1 cut(s) 402
BtsCI GGATG 3 cut(s) 14, 103, 412
BtsI GCAGTG 1 cut(s) 85
BtsIMutI CAGTG 3 cut(s) 85, 433, 445
CaiI CAGNNNCTG 1 cut(s) 432
CfoI GCGC 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 169
ClaI ATCGAT 1 cut(s) 264
Csp6I GTAC 2 cut(s) 227, 465
CviAII CATG 3 cut(s) 120, 403, 481
CviJI RGCY 6 cut(s) 201, 236, 401, 432, 444, 521
CviKI_1 RGCY 6 cut(s) 201, 236, 401, 432, 444, 521
CviQI GTAC 2 cut(s) 227, 465
DdeI CTNAG 2 cut(s) 202, 230
DpnI GATC 3 cut(s) 263, 267, 501
DpnII GATC 3 cut(s) 261, 265, 499
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
Eco130I CCWWGG 2 cut(s) 165, 402
Eco47I GGWCC 1 cut(s) 169
EcoO109I RGGNCCY 1 cut(s) 169
EcoT14I CCWWGG 2 cut(s) 165, 402
ErhI CCWWGG 2 cut(s) 165, 402
FaeI CATG 3 cut(s) 123, 406, 484
FaiI YATR 7 cut(s) 35, 37, 67, 121, 404, 482, 524
FaqI GGGAC 1 cut(s) 155
FatI CATG 3 cut(s) 119, 402, 480
FauI CCCGC 1 cut(s) 148
FauNDI CATATG 1 cut(s) 35
Fnu4HI GCNGC 5 cut(s) 24, 76, 124, 393, 530
FokI GGATG 2 cut(s) 110, 419
Fsp4HI GCNGC 5 cut(s) 24, 76, 124, 393, 530
FspBI CTAG 1 cut(s) 20
GlaI GCGC 1 cut(s) 181
GluI GCNGC 5 cut(s) 24, 76, 124, 393, 530
HaeII RGCGCY 1 cut(s) 183
HapII CCGG 1 cut(s) 462
HhaI GCGC 1 cut(s) 182
Hin1II CATG 3 cut(s) 123, 406, 484
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HinfI GANTC 3 cut(s) 7, 155, 354
HpaII CCGG 1 cut(s) 462
Hpy166II GTNNAC 2 cut(s) 253, 314
Hpy188I TCNGA 2 cut(s) 381, 474
Hpy188III TCNNGA 1 cut(s) 540
Hpy8I GTNNAC 2 cut(s) 253, 314
Hpy99I CGWCG 2 cut(s) 224, 227
HpyAV CCTTC 4 cut(s) 99, 356, 364, 382
HpyCH4III ACNGT 4 cut(s) 297, 415, 440, 511
HpyCH4IV ACGT 2 cut(s) 192, 225
HpyCH4V TGCA 4 cut(s) 123, 188, 314, 529
HpyF10VI GCNNNNNNNGC 5 cut(s) 75, 277, 398, 457, 503
HpyF3I CTNAG 2 cut(s) 202, 230
HpySE526I ACGT 2 cut(s) 192, 225
Hsp92II CATG 3 cut(s) 123, 406, 484
HspAI GCGC 1 cut(s) 180
Kzo9I GATC 3 cut(s) 261, 265, 499
LpnPI CCDG 5 cut(s) 217, 272, 301, 302, 475
Lsp1109I GCAGC 2 cut(s) 35, 135
MaeI CTAG 1 cut(s) 20
MaeII ACGT 2 cut(s) 192, 225
MaeIII GTNAC 2 cut(s) 434, 511
MalI GATC 3 cut(s) 263, 267, 501
MboI GATC 3 cut(s) 261, 265, 499
MboII GAAGA 3 cut(s) 16, 47, 50
MfeI CAATTG 1 cut(s) 533
MflI RGATCY 1 cut(s) 499
MhlI GDGCHC 1 cut(s) 316
MluCI AATT 2 cut(s) 48, 533
MlyI GAGTC 3 cut(s) 16, 164, 363
MnlI CCTC 3 cut(s) 155, 183, 480
MseI TTAA 1 cut(s) 276
MspI CCGG 1 cut(s) 462
MspR9I CCNGG 1 cut(s) 462
MunI CAATTG 1 cut(s) 533
MwoI GCNNNNNNNGC 5 cut(s) 75, 277, 398, 457, 503
NciI CCSGG 1 cut(s) 462
NcoI CCATGG 1 cut(s) 402
NdeI CATATG 1 cut(s) 35
NdeII GATC 3 cut(s) 261, 265, 499
NlaIII CATG 3 cut(s) 123, 406, 484
NlaIV GGNNCC 2 cut(s) 171, 286
NmuCI GTSAC 1 cut(s) 511
NspI RCATGY 1 cut(s) 123
NspV TTCGAA 1 cut(s) 386
PkrI GCNGC 5 cut(s) 25, 77, 125, 394, 531
PleI GAGTC 3 cut(s) 15, 163, 362
PpsI GAGTC 3 cut(s) 15, 163, 362
PpuMI RGGWCCY 1 cut(s) 169
PshAI GACNNNNGTC 1 cut(s) 295
Psp5II RGGWCCY 1 cut(s) 169
PspN4I GGNNCC 2 cut(s) 171, 286
PspPI GGNCC 1 cut(s) 169
PspPPI RGGWCCY 1 cut(s) 169
PstNI CAGNNNCTG 1 cut(s) 432
PsuI RGATCY 1 cut(s) 499
RsaI GTAC 2 cut(s) 228, 466
RsaNI GTAC 2 cut(s) 227, 465
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 5 cut(s) 24, 76, 124, 393, 530
Sau3AI GATC 3 cut(s) 261, 265, 499
Sau96I GGNCC 1 cut(s) 169
SchI GAGTC 3 cut(s) 16, 164, 363
ScrFI CCNGG 1 cut(s) 462
SduI GDGCHC 1 cut(s) 316
SetI ASST 5 cut(s) 136, 171, 195, 228, 523
SfcI CTRYAG 1 cut(s) 522
SfuI TTCGAA 1 cut(s) 386
SinI GGWCC 1 cut(s) 169
Sse9I AATT 2 cut(s) 48, 533
SsiI CCGC 4 cut(s) 76, 141, 392, 497
SspMI CTAG 1 cut(s) 20
StyD4I CCNGG 1 cut(s) 460
StyI CCWWGG 2 cut(s) 165, 402
TaaI ACNGT 4 cut(s) 297, 415, 440, 511
TaiI ACGT 2 cut(s) 195, 228
TaqI TCGA 5 cut(s) 92, 153, 264, 357, 386
TasI AATT 2 cut(s) 48, 533
TauI GCSGC 2 cut(s) 78, 395
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TscAI CASTG 3 cut(s) 85, 433, 445
TseFI GTSAC 1 cut(s) 511
TseI GCWGC 3 cut(s) 23, 123, 529
Tsp45I GTSAC 1 cut(s) 511
TspDTI ATGAA 4 cut(s) 17, 230, 375, 497
TspRI CASTG 3 cut(s) 85, 433, 445
VneI GTGCAC 1 cut(s) 312
VpaK11BI GGWCC 1 cut(s) 169
XapI RAATTY 1 cut(s) 48
XceI RCATGY 1 cut(s) 123
XspI CTAG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.