Rh4DG313300

cell wall vacuolar inhibitor of fructosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0015605
Physical Location & Seq
Reverse (-)
53573457 .. 53573906
450 bp
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UTR
Exon/CDS
Intron
Rh4DG313300.1

Sequence Viewer

Length: 450 bp
ATGGATGTGAATCTCATCGAGGAAACATGCCGCAACACACCAAAACCCAAACTTTGTGTCGATTCTCTTTACTCGGTTCCTCAGAGCGCCAGTGCAGACGTCAAAGGCTTAGCGCACATAATGAATGACGTCGTACTCAGGACCAAAGCACAAGAAGTTCATACCAAGATCGATCTGCTTATTAAGCAGGGCACTAGACCACAGTCATTACTTACATGCGCACAGACTTATAGCCTAATTATAAATGATAAAATCAAAGCAGTCAATGATGGATTGAAGGATGATCAGCCTTTGAAGACGGAGCAAGCCATGGTTGATACTGTCTCTTTGCTCAATGACTGTAACAGTGGTTTGGAACATTCTTTGCCCGGCACTCCTCTGATTGGTATGAACACCAATGTGGCTGATCTTGCGACTGTGACTAAAGCAATAGTGAAACAATTGCCTTGA

Protein Analysis

149

Amino Acids

16.16

Weight (kDa)

5.86

Isoelectric Point (pI)

26.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 4 - 144 5.7e-13 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 242
AatII GACGTC 2 cut(s) 102, 132
Acc16I TGCGCA 1 cut(s) 220
AciI CCGC 1 cut(s) 31
AcyI GRCGYC 2 cut(s) 99, 129
AfaI GTAC 1 cut(s) 135
AfiI CCNNNNNNNGG 1 cut(s) 383
AgsI TTSAA 2 cut(s) 277, 295
AleI CACNNNNGTG 1 cut(s) 398
Alw26I GTCTC 1 cut(s) 328
ArsI GACNNNNNNTTYG 2 cut(s) 42, 74
AspLEI GCGC 3 cut(s) 89, 115, 221
AspS9I GGNCC 1 cut(s) 141
AsuC2I CCSGG 1 cut(s) 369
AvaII GGWCC 1 cut(s) 141
BaeGI GKGCMC 1 cut(s) 194
BbsI GAAGAC 1 cut(s) 302
BccI CCATC 1 cut(s) 263
BclI TGATCA 1 cut(s) 283
BcnI CCSGG 1 cut(s) 369
BcoDI GTCTC 1 cut(s) 328
BfaI CTAG 1 cut(s) 195
BfoI RGCGCY 1 cut(s) 90
BisI GCNGC 1 cut(s) 31
BlpI GCTNAGC 1 cut(s) 109
BlsI GCNGC 1 cut(s) 32
Bme1390I CCNGG 1 cut(s) 369
Bme18I GGWCC 1 cut(s) 141
BmgT120I GGNCC 1 cut(s) 141
BmiI GGNNCC 1 cut(s) 78
BmrFI CCNGG 1 cut(s) 369
BoxI GACNNNNGTC 1 cut(s) 202
BpiI GAAGAC 1 cut(s) 302
Bpu1102I GCTNAGC 1 cut(s) 109
BpuMI CCSGG 1 cut(s) 369
Bsa29I ATCGAT 1 cut(s) 171
BsaBI GATNNNNATC 1 cut(s) 9
BsaHI GRCGYC 2 cut(s) 99, 129
BsaJI CCNNGG 1 cut(s) 309
Bsc4I CCNNNNNNNGG 1 cut(s) 383
Bse1I ACTGG 1 cut(s) 90
Bse8I GATNNNNATC 1 cut(s) 9
BseCI ATCGAT 1 cut(s) 171
BseDI CCNNGG 1 cut(s) 309
BseGI GGATG 2 cut(s) 10, 286
BseJI GATNNNNATC 1 cut(s) 9
BseLI CCNNNNNNNGG 1 cut(s) 383
BseMII CTCAG 2 cut(s) 95, 151
BseNI ACTGG 1 cut(s) 90
BseRI GAGGAG 1 cut(s) 366
BseSI GKGCMC 1 cut(s) 194
BsgI GTGCAG 1 cut(s) 114
BshVI ATCGAT 1 cut(s) 171
BsiSI CCGG 1 cut(s) 369
BslI CCNNNNNNNGG 1 cut(s) 383
BsmAI GTCTC 1 cut(s) 328
Bsp1286I GDGCHC 1 cut(s) 194
Bsp143I GATC 4 cut(s) 168, 172, 283, 406
Bsp1720I GCTNAGC 1 cut(s) 109
Bsp19I CCATGG 1 cut(s) 309
BspACI CCGC 1 cut(s) 31
BspCNI CTCAG 2 cut(s) 94, 150
BspDI ATCGAT 1 cut(s) 171
BspLI GGNNCC 1 cut(s) 78
BsrI ACTGG 1 cut(s) 90
BssECI CCNNGG 1 cut(s) 309
BssMI GATC 4 cut(s) 168, 172, 283, 406
BssNI GRCGYC 2 cut(s) 99, 129
BssT1I CCWWGG 1 cut(s) 309
Bst4CI ACNGT 5 cut(s) 204, 322, 341, 347, 418
BstACI GRCGYC 2 cut(s) 99, 129
BstC8I GCNNGC 1 cut(s) 306
BstDEI CTNAG 3 cut(s) 81, 109, 137
BstDSI CCRYGG 1 cut(s) 309
BstF5I GGATG 2 cut(s) 10, 286
BstH2I RGCGCY 1 cut(s) 90
BstHHI GCGC 3 cut(s) 89, 115, 221
BstKTI GATC 4 cut(s) 171, 175, 286, 409
BstMAI GTCTC 1 cut(s) 328
BstMBI GATC 4 cut(s) 168, 172, 283, 406
BstMWI GCNNNNNNNGC 2 cut(s) 184, 410
BstNSI RCATGY 2 cut(s) 30, 219
BstPAI GACNNNNGTC 1 cut(s) 202
BstSCI CCNGG 1 cut(s) 367
BstSLI GKGCMC 1 cut(s) 194
BstV2I GAAGAC 1 cut(s) 302
Bsu15I ATCGAT 1 cut(s) 171
BsuTUI ATCGAT 1 cut(s) 171
BtgI CCRYGG 1 cut(s) 309
BtsCI GGATG 2 cut(s) 10, 286
BtsIMutI CAGTG 2 cut(s) 97, 352
Cac8I GCNNGC 1 cut(s) 306
CfoI GCGC 3 cut(s) 89, 115, 221
Cfr13I GGNCC 1 cut(s) 141
ClaI ATCGAT 1 cut(s) 171
Csp6I GTAC 1 cut(s) 134
CviAII CATG 3 cut(s) 27, 216, 310
CviJI RGCY 5 cut(s) 108, 234, 289, 308, 404
CviKI_1 RGCY 5 cut(s) 108, 234, 289, 308, 404
CviQI GTAC 1 cut(s) 134
DdeI CTNAG 3 cut(s) 81, 109, 137
DpnI GATC 4 cut(s) 170, 174, 285, 408
DpnII GATC 4 cut(s) 168, 172, 283, 406
Eco130I CCWWGG 1 cut(s) 309
Eco47I GGWCC 1 cut(s) 141
EcoT14I CCWWGG 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 309
FaeI CATG 3 cut(s) 30, 219, 313
FaiI YATR 8 cut(s) 28, 119, 162, 217, 231, 242, 311, 389
FatI CATG 3 cut(s) 26, 215, 309
FbaI TGATCA 1 cut(s) 283
Fnu4HI GCNGC 1 cut(s) 31
FokI GGATG 2 cut(s) 17, 293
Fsp4HI GCNGC 1 cut(s) 31
FspAI RTGCGCAY 1 cut(s) 220
FspBI CTAG 1 cut(s) 195
FspI TGCGCA 1 cut(s) 220
GlaI GCGC 3 cut(s) 88, 114, 220
GluI GCNGC 1 cut(s) 31
HaeII RGCGCY 1 cut(s) 90
HapII CCGG 1 cut(s) 369
HhaI GCGC 3 cut(s) 89, 115, 221
Hin1I GRCGYC 2 cut(s) 99, 129
Hin1II CATG 3 cut(s) 30, 219, 313
Hin6I GCGC 3 cut(s) 87, 113, 219
HinP1I GCGC 3 cut(s) 87, 113, 219
HinfI GANTC 2 cut(s) 10, 62
HpaII CCGG 1 cut(s) 369
Hpy188I TCNGA 2 cut(s) 84, 381
Hpy188III TCNNGA 1 cut(s) 139
Hpy99I CGWCG 1 cut(s) 134
HpyAV CCTTC 1 cut(s) 271
HpyCH4III ACNGT 5 cut(s) 204, 322, 341, 347, 418
HpyCH4IV ACGT 2 cut(s) 99, 129
HpyCH4V TGCA 1 cut(s) 95
HpyF10VI GCNNNNNNNGC 2 cut(s) 184, 410
HpyF3I CTNAG 3 cut(s) 81, 109, 137
HpySE526I ACGT 2 cut(s) 99, 129
Hsp92I GRCGYC 2 cut(s) 99, 129
Hsp92II CATG 3 cut(s) 30, 219, 313
HspAI GCGC 3 cut(s) 87, 113, 219
Ksp22I TGATCA 1 cut(s) 283
Kzo9I GATC 4 cut(s) 168, 172, 283, 406
LmnI GCTCC 1 cut(s) 301
LpnPI CCDG 4 cut(s) 103, 124, 173, 382
MaeI CTAG 1 cut(s) 195
MaeII ACGT 2 cut(s) 99, 129
MaeIII GTNAC 2 cut(s) 341, 418
MalI GATC 4 cut(s) 170, 174, 285, 408
MboI GATC 4 cut(s) 168, 172, 283, 406
MboII GAAGA 1 cut(s) 307
MfeI CAATTG 1 cut(s) 440
MhlI GDGCHC 1 cut(s) 194
MluCI AATT 2 cut(s) 237, 440
MnlI CCTC 3 cut(s) 13, 90, 387
MseI TTAA 1 cut(s) 183
MslI CAYNNNNRTG 1 cut(s) 398
MspI CCGG 1 cut(s) 369
MspR9I CCNGG 1 cut(s) 369
MunI CAATTG 1 cut(s) 440
MwoI GCNNNNNNNGC 2 cut(s) 184, 410
NciI CCSGG 1 cut(s) 369
NcoI CCATGG 1 cut(s) 309
NdeII GATC 4 cut(s) 168, 172, 283, 406
NlaIII CATG 3 cut(s) 30, 219, 313
NlaIV GGNNCC 1 cut(s) 78
NmuCI GTSAC 1 cut(s) 418
NsbI TGCGCA 1 cut(s) 220
NspI RCATGY 2 cut(s) 30, 219
OliI CACNNNNGTG 1 cut(s) 398
PfeI GAWTC 2 cut(s) 10, 62
PkrI GCNGC 1 cut(s) 32
PshAI GACNNNNGTC 1 cut(s) 202
PsiI TTATAA 1 cut(s) 242
PspN4I GGNNCC 1 cut(s) 78
PspPI GGNCC 1 cut(s) 141
RsaI GTAC 1 cut(s) 135
RsaNI GTAC 1 cut(s) 134
RseI CAYNNNNRTG 1 cut(s) 398
SaqAI TTAA 1 cut(s) 183
SatI GCNGC 1 cut(s) 31
Sau3AI GATC 4 cut(s) 168, 172, 283, 406
Sau96I GGNCC 1 cut(s) 141
ScrFI CCNGG 1 cut(s) 369
SduI GDGCHC 1 cut(s) 194
SetI ASST 2 cut(s) 102, 132
SinI GGWCC 1 cut(s) 141
SmiMI CAYNNNNRTG 1 cut(s) 398
Sse9I AATT 2 cut(s) 237, 440
SsiI CCGC 1 cut(s) 31
SspMI CTAG 1 cut(s) 195
StyD4I CCNGG 1 cut(s) 367
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 5 cut(s) 204, 322, 341, 347, 418
TaiI ACGT 2 cut(s) 102, 132
TaqI TCGA 3 cut(s) 18, 60, 171
TasI AATT 2 cut(s) 237, 440
TauI GCSGC 1 cut(s) 33
TfiI GAWTC 2 cut(s) 10, 62
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TscAI CASTG 2 cut(s) 97, 352
TseFI GTSAC 1 cut(s) 418
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 3 cut(s) 137, 149, 404
TspGWI ACGGA 1 cut(s) 314
TspRI CASTG 2 cut(s) 97, 352
VpaK11BI GGWCC 1 cut(s) 141
XceI RCATGY 2 cut(s) 30, 219
XspI CTAG 1 cut(s) 195
ZraI GACGTC 2 cut(s) 100, 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.