Rroxscaffold_5G00373510

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015605
Physical Location & Seq
Reverse (-)
54706992 .. 54707534
543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00373510.1

Sequence Viewer

Length: 543 bp
ATGAAGAGTGTAACATCCGTAGCTGCAGTTCTCATGTGTTTCTTTCAAATTTCGTTTATTCCAATATGTCATTGCGGCCGTGTTTCCCCTCCCATGGATGTGAATCTCATCAAAGAAACATGCCACAACACACCAAAACCCAAACTTTGTGTCAATTCTCTTTACTCGGTTCCTCAGAGCGCCAGTGCAGATGTCAAAGGCTTAGCGCACATAATGAATGATGTCGTACTTAGGACCAAAGCACAAGAAGTTCATACCAAGATCGATCTGCTTATTAAGCAGGGCACTAGACCACAGTCATTACTCACATGCGCACAGACTTATAGCCTAATTATAAATGATAAAATCAAAGCAGTCAATGATGGATTGAAGGATGATCATCCTTTGAAGGCGGAGCAAGCCATGGGTGATACTATCACTTTGCTCAATGACTGTAACAGTGGTTTGGAACATTCTTTGCCTGACACTCCTCTGATTGGCATGAACACCAATGTGGCTGATCTTGCGACTGTGACTAAAGCAATAGTGAAACAATTGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

180

Amino Acids

19.52

Weight (kDa)

7.61

Isoelectric Point (pI)

23.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 35 - 175 1.1e-14 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 335
Acc16I TGCGCA 1 cut(s) 313
AciI CCGC 2 cut(s) 75, 392
AcoI YGGCCR 1 cut(s) 76
AcsI RAATTY 1 cut(s) 48
AfaI GTAC 1 cut(s) 228
AfiI CCNNNNNNNGG 2 cut(s) 94, 476
AgsI TTSAA 3 cut(s) 47, 370, 388
AleI CACNNNNGTG 1 cut(s) 491
AluBI AGCT 1 cut(s) 23
AluI AGCT 1 cut(s) 23
AoxI GGCC 1 cut(s) 76
ApeKI GCWGC 1 cut(s) 23
ApoI RAATTY 1 cut(s) 48
ArsI GACNNNNNNTTYG 2 cut(s) 135, 167
AspLEI GCGC 3 cut(s) 182, 208, 314
AspS9I GGNCC 1 cut(s) 234
AsuHPI GGTGA 1 cut(s) 419
AvaII GGWCC 1 cut(s) 234
BaeGI GKGCMC 1 cut(s) 287
BbvI GCAGC 1 cut(s) 10
BccI CCATC 1 cut(s) 356
BceAI ACGGC 1 cut(s) 63
BclI TGATCA 1 cut(s) 376
BfaI CTAG 1 cut(s) 288
BfmI CTRYAG 1 cut(s) 24
BfoI RGCGCY 1 cut(s) 183
BisI GCNGC 2 cut(s) 24, 76
BlpI GCTNAGC 1 cut(s) 202
BlsI GCNGC 2 cut(s) 25, 77
Bme18I GGWCC 1 cut(s) 234
BmgT120I GGNCC 1 cut(s) 234
BmiI GGNNCC 1 cut(s) 171
BoxI GACNNNNGTC 1 cut(s) 295
Bpu1102I GCTNAGC 1 cut(s) 202
Bsa29I ATCGAT 1 cut(s) 264
BsaBI GATNNNNATC 2 cut(s) 102, 378
BsaJI CCNNGG 2 cut(s) 93, 402
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 476
Bse1I ACTGG 1 cut(s) 183
Bse3DI GCAATG 1 cut(s) 70
Bse8I GATNNNNATC 2 cut(s) 102, 378
BseCI ATCGAT 1 cut(s) 264
BseDI CCNNGG 2 cut(s) 93, 402
BseGI GGATG 4 cut(s) 14, 103, 379, 379
BseJI GATNNNNATC 2 cut(s) 102, 378
BseLI CCNNNNNNNGG 2 cut(s) 94, 476
BseMI GCAATG 1 cut(s) 70
BseMII CTCAG 1 cut(s) 188
BseNI ACTGG 1 cut(s) 183
BseRI GAGGAG 1 cut(s) 459
BseSI GKGCMC 1 cut(s) 287
BseX3I CGGCCG 1 cut(s) 76
BseXI GCAGC 1 cut(s) 10
BsgI GTGCAG 1 cut(s) 207
Bsh1285I CGRYCG 1 cut(s) 79
BshFI GGCC 1 cut(s) 78
BshVI ATCGAT 1 cut(s) 264
BsiEI CGRYCG 1 cut(s) 79
BslI CCNNNNNNNGG 2 cut(s) 94, 476
BsnI GGCC 1 cut(s) 78
Bsp1286I GDGCHC 1 cut(s) 287
Bsp143I GATC 4 cut(s) 261, 265, 376, 499
Bsp1720I GCTNAGC 1 cut(s) 202
Bsp19I CCATGG 2 cut(s) 93, 402
BspACI CCGC 2 cut(s) 75, 392
BspANI GGCC 1 cut(s) 78
BspCNI CTCAG 1 cut(s) 187
BspDI ATCGAT 1 cut(s) 264
BspLI GGNNCC 1 cut(s) 171
BspMAI CTGCAG 1 cut(s) 28
BsrDI GCAATG 1 cut(s) 70
BsrI ACTGG 1 cut(s) 183
BssECI CCNNGG 2 cut(s) 93, 402
BssMI GATC 4 cut(s) 261, 265, 376, 499
BssT1I CCWWGG 2 cut(s) 93, 402
Bst4CI ACNGT 4 cut(s) 297, 434, 440, 511
BstC8I GCNNGC 1 cut(s) 399
BstDEI CTNAG 3 cut(s) 174, 202, 230
BstDSI CCRYGG 2 cut(s) 93, 402
BstF5I GGATG 4 cut(s) 14, 103, 379, 379
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 3 cut(s) 182, 208, 314
BstKTI GATC 4 cut(s) 264, 268, 379, 502
BstMBI GATC 4 cut(s) 261, 265, 376, 499
BstMCI CGRYCG 1 cut(s) 79
BstMWI GCNNNNNNNGC 3 cut(s) 277, 398, 503
BstNSI RCATGY 2 cut(s) 123, 312
BstPAI GACNNNNGTC 1 cut(s) 295
BstSFI CTRYAG 1 cut(s) 24
BstSLI GKGCMC 1 cut(s) 287
BstV1I GCAGC 1 cut(s) 10
BstZI CGGCCG 1 cut(s) 76
Bsu15I ATCGAT 1 cut(s) 264
BsuRI GGCC 1 cut(s) 78
BsuTUI ATCGAT 1 cut(s) 264
BtgI CCRYGG 2 cut(s) 93, 402
BtsCI GGATG 4 cut(s) 14, 103, 379, 379
BtsIMutI CAGTG 2 cut(s) 190, 445
Cac8I GCNNGC 1 cut(s) 399
CfoI GCGC 3 cut(s) 182, 208, 314
Cfr13I GGNCC 1 cut(s) 234
ClaI ATCGAT 1 cut(s) 264
Csp6I GTAC 1 cut(s) 227
CviAII CATG 6 cut(s) 34, 94, 120, 309, 403, 481
CviJI RGCY 6 cut(s) 23, 78, 201, 327, 401, 497
CviKI_1 RGCY 6 cut(s) 23, 78, 201, 327, 401, 497
CviQI GTAC 1 cut(s) 227
DdeI CTNAG 3 cut(s) 174, 202, 230
DpnI GATC 4 cut(s) 263, 267, 378, 501
DpnII GATC 4 cut(s) 261, 265, 376, 499
EaeI YGGCCR 1 cut(s) 76
EagI CGGCCG 1 cut(s) 76
EciI GGCGGA 1 cut(s) 407
EclXI CGGCCG 1 cut(s) 76
Eco130I CCWWGG 2 cut(s) 93, 402
Eco47I GGWCC 1 cut(s) 234
Eco52I CGGCCG 1 cut(s) 76
EcoT14I CCWWGG 2 cut(s) 93, 402
ErhI CCWWGG 2 cut(s) 93, 402
FaeI CATG 6 cut(s) 37, 97, 123, 312, 406, 484
FatI CATG 6 cut(s) 33, 93, 119, 308, 402, 480
FbaI TGATCA 1 cut(s) 376
Fnu4HI GCNGC 2 cut(s) 24, 76
FokI GGATG 3 cut(s) 110, 366, 386
Fsp4HI GCNGC 2 cut(s) 24, 76
FspAI RTGCGCAY 1 cut(s) 313
FspBI CTAG 1 cut(s) 288
FspI TGCGCA 1 cut(s) 313
GlaI GCGC 3 cut(s) 181, 207, 313
GluI GCNGC 2 cut(s) 24, 76
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 1 cut(s) 78
HhaI GCGC 3 cut(s) 182, 208, 314
Hin1II CATG 6 cut(s) 37, 97, 123, 312, 406, 484
Hin6I GCGC 3 cut(s) 180, 206, 312
HinP1I GCGC 3 cut(s) 180, 206, 312
HinfI GANTC 1 cut(s) 103
HphI GGTGA 1 cut(s) 419
Hpy188I TCNGA 2 cut(s) 177, 474
HpyAV CCTTC 2 cut(s) 364, 382
HpyCH4III ACNGT 4 cut(s) 297, 434, 440, 511
HpyCH4V TGCA 2 cut(s) 26, 188
HpyF10VI GCNNNNNNNGC 3 cut(s) 277, 398, 503
HpyF3I CTNAG 3 cut(s) 174, 202, 230
Hsp92II CATG 6 cut(s) 37, 97, 123, 312, 406, 484
HspAI GCGC 3 cut(s) 180, 206, 312
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 4 cut(s) 261, 265, 376, 499
LmnI GCTCC 1 cut(s) 394
LpnPI CCDG 3 cut(s) 196, 266, 474
Lsp1109I GCAGC 1 cut(s) 10
MaeI CTAG 1 cut(s) 288
MaeIII GTNAC 3 cut(s) 10, 434, 511
MalI GATC 4 cut(s) 263, 267, 378, 501
MboI GATC 4 cut(s) 261, 265, 376, 499
MboII GAAGA 1 cut(s) 16
MfeI CAATTG 1 cut(s) 533
MhlI GDGCHC 1 cut(s) 287
MluCI AATT 4 cut(s) 48, 154, 330, 533
MnlI CCTC 3 cut(s) 99, 183, 480
MseI TTAA 1 cut(s) 276
MslI CAYNNNNRTG 2 cut(s) 98, 491
MunI CAATTG 1 cut(s) 533
MwoI GCNNNNNNNGC 3 cut(s) 277, 398, 503
NcoI CCATGG 2 cut(s) 93, 402
NdeII GATC 4 cut(s) 261, 265, 376, 499
NlaIII CATG 6 cut(s) 37, 97, 123, 312, 406, 484
NlaIV GGNNCC 1 cut(s) 171
NmuCI GTSAC 1 cut(s) 511
NsbI TGCGCA 1 cut(s) 313
NspI RCATGY 2 cut(s) 123, 312
OliI CACNNNNGTG 1 cut(s) 491
PfeI GAWTC 1 cut(s) 103
PkrI GCNGC 2 cut(s) 25, 77
PshAI GACNNNNGTC 1 cut(s) 295
PsiI TTATAA 1 cut(s) 335
PspN4I GGNNCC 1 cut(s) 171
PspPI GGNCC 1 cut(s) 234
PsrI GAACNNNNNNTAC 2 cut(s) 12, 44
PstI CTGCAG 1 cut(s) 28
RsaI GTAC 1 cut(s) 228
RsaNI GTAC 1 cut(s) 227
RseI CAYNNNNRTG 2 cut(s) 98, 491
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 2 cut(s) 24, 76
Sau3AI GATC 4 cut(s) 261, 265, 376, 499
Sau96I GGNCC 1 cut(s) 234
SduI GDGCHC 1 cut(s) 287
SetI ASST 1 cut(s) 25
SfcI CTRYAG 1 cut(s) 24
SinI GGWCC 1 cut(s) 234
SmiMI CAYNNNNRTG 2 cut(s) 98, 491
Sse9I AATT 4 cut(s) 48, 154, 330, 533
SsiI CCGC 2 cut(s) 75, 392
SspMI CTAG 1 cut(s) 288
StyI CCWWGG 2 cut(s) 93, 402
TaaI ACNGT 4 cut(s) 297, 434, 440, 511
TaqI TCGA 1 cut(s) 264
TasI AATT 4 cut(s) 48, 154, 330, 533
TauI GCSGC 1 cut(s) 78
TfiI GAWTC 1 cut(s) 103
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TscAI CASTG 2 cut(s) 190, 445
TseFI GTSAC 1 cut(s) 511
TseI GCWGC 1 cut(s) 23
Tsp45I GTSAC 1 cut(s) 511
TspDTI ATGAA 4 cut(s) 17, 230, 242, 497
TspGWI ACGGA 1 cut(s) 7
TspRI CASTG 2 cut(s) 190, 445
VpaK11BI GGWCC 1 cut(s) 234
XapI RAATTY 1 cut(s) 48
XceI RCATGY 2 cut(s) 123, 312
XspI CTAG 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.