Rh4DG317200

Cell wall vacuolar inhibitor of fructosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0015605
Physical Location & Seq
Forward (+)
53997499 .. 53998104
606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG317200.1

Sequence Viewer

Length: 546 bp
ATGAAAAGTATAATATCTGTAGCAGTTCTCATATGCCTCTTTGAAGTTTCATTTCTTCCATTAACTCAGTGCAGGGTTTACCTACCAACGGATGCCAATCTCATTGGCCAGACATGCCTTCAAACCCCGAAACCCGATCTTTGTGTCTCTTCTCTTGAATCGGTCCCTGAGAGCTCCGATACAGACATCAAAGGCTTAGCTCTCATAATGTCCGACAAAGTACTCAAGGGCAAAGCACAGGATGCTCTGAAAGAAATCAACGAGCTGATTAAGCAGACACCTATATCGAGTAACAAAGCCTTACTTGCATGCGCAGACTACTATGGCACGATCATAGAATTTCATATCGAAAAACAAGCGCATGATGGAATTACTGGGAATCAGCCTAGGAAAGCTGCACAGCAAGCCATGGATGATACCGTCTATTTGGTCAATGAATGTAACAATGGTTTGGGTTCTTCACCTAGCTCTCCTCTAACAGTTATGGGCGCAAGCGTGGCTGATATTGCGACCATTACTAAAGCAATAGTGCAGATATTGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

181

Amino Acids

19.35

Weight (kDa)

4.95

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 33 - 154 9.6e-14 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 313
AcoI YGGCCR 1 cut(s) 106
AcsI RAATTY 1 cut(s) 338
AfaI GTAC 1 cut(s) 222
AfiI CCNNNNNNNGG 1 cut(s) 88
AgsI TTSAA 3 cut(s) 44, 122, 158
AluBI AGCT 5 cut(s) 174, 200, 265, 395, 468
AluI AGCT 5 cut(s) 174, 200, 265, 395, 468
Alw21I GWGCWC 1 cut(s) 176
Alw26I GTCTC 1 cut(s) 151
AoxI GGCC 1 cut(s) 106
ApeKI GCWGC 1 cut(s) 395
ApoI RAATTY 1 cut(s) 338
AspA2I CCTAGG 1 cut(s) 386
AspLEI GCGC 3 cut(s) 314, 361, 491
AspS9I GGNCC 1 cut(s) 163
AsuHPI GGTGA 1 cut(s) 453
AvaII GGWCC 1 cut(s) 163
AvrII CCTAGG 1 cut(s) 386
BalI TGGCCA 1 cut(s) 108
BanII GRGCYC 1 cut(s) 176
Bbv12I GWGCWC 1 cut(s) 176
BbvI GCAGC 1 cut(s) 382
BccI CCATC 1 cut(s) 359
BcoDI GTCTC 1 cut(s) 151
BfaI CTAG 2 cut(s) 387, 465
BfmI CTRYAG 1 cut(s) 18
BisI GCNGC 1 cut(s) 396
BlnI CCTAGG 1 cut(s) 386
BlpI GCTNAGC 1 cut(s) 196
BlsI GCNGC 1 cut(s) 397
BmcAI AGTACT 1 cut(s) 222
Bme18I GGWCC 1 cut(s) 163
BmgT120I GGNCC 1 cut(s) 163
BmiI GGNNCC 1 cut(s) 165
BmrI ACTGGG 1 cut(s) 384
BmsI GCATC 2 cut(s) 82, 232
BmuI ACTGGG 1 cut(s) 384
Bpu1102I GCTNAGC 1 cut(s) 196
BpuEI CTTGAG 1 cut(s) 209
BsaBI GATNNNNATC 1 cut(s) 96
BsaJI CCNNGG 2 cut(s) 386, 408
Bsc4I CCNNNNNNNGG 1 cut(s) 88
Bse1I ACTGG 1 cut(s) 379
Bse8I GATNNNNATC 1 cut(s) 96
BseDI CCNNGG 2 cut(s) 386, 408
BseGI GGATG 3 cut(s) 97, 247, 418
BseJI GATNNNNATC 1 cut(s) 96
BseLI CCNNNNNNNGG 1 cut(s) 88
BseMII CTCAG 2 cut(s) 80, 159
BseNI ACTGG 1 cut(s) 379
BseRI GAGGAG 1 cut(s) 462
BseXI GCAGC 1 cut(s) 382
BsgI GTGCAG 2 cut(s) 91, 381
BshFI GGCC 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 176
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 1 cut(s) 88
BsmAI GTCTC 1 cut(s) 151
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 1 cut(s) 108
Bsp1286I GDGCHC 1 cut(s) 176
Bsp143I GATC 2 cut(s) 136, 330
Bsp1720I GCTNAGC 1 cut(s) 196
Bsp19I CCATGG 1 cut(s) 408
BspANI GGCC 1 cut(s) 108
BspCNI CTCAG 2 cut(s) 79, 160
BspLI GGNNCC 1 cut(s) 165
BsrI ACTGG 1 cut(s) 379
BssECI CCNNGG 2 cut(s) 386, 408
BssMI GATC 2 cut(s) 136, 330
BssT1I CCWWGG 2 cut(s) 386, 408
Bst4CI ACNGT 2 cut(s) 421, 481
Bst6I CTCTTC 1 cut(s) 154
BstAPI GCANNNNNTGC 1 cut(s) 242
BstC8I GCNNGC 3 cut(s) 310, 405, 493
BstDEI CTNAG 3 cut(s) 66, 168, 196
BstDSI CCRYGG 1 cut(s) 408
BstF5I GGATG 3 cut(s) 97, 247, 418
BstHHI GCGC 3 cut(s) 314, 361, 491
BstKTI GATC 2 cut(s) 139, 333
BstMAI GTCTC 1 cut(s) 151
BstMBI GATC 2 cut(s) 136, 330
BstMWI GCNNNNNNNGC 7 cut(s) 114, 242, 271, 305, 404, 497, 506
BstNSI RCATGY 2 cut(s) 117, 312
BstSFI CTRYAG 1 cut(s) 18
BstV1I GCAGC 1 cut(s) 382
BsuRI GGCC 1 cut(s) 108
BtgI CCRYGG 1 cut(s) 408
BtsCI GGATG 3 cut(s) 97, 247, 418
BtsIMutI CAGTG 1 cut(s) 74
Cac8I GCNNGC 3 cut(s) 310, 405, 493
CfoI GCGC 3 cut(s) 314, 361, 491
Cfr13I GGNCC 1 cut(s) 163
Csp6I GTAC 1 cut(s) 221
CviAII CATG 4 cut(s) 114, 309, 362, 409
CviQI GTAC 1 cut(s) 221
DdeI CTNAG 3 cut(s) 66, 168, 196
DpnI GATC 2 cut(s) 138, 332
DpnII GATC 2 cut(s) 136, 330
EaeI YGGCCR 1 cut(s) 106
Eam1104I CTCTTC 1 cut(s) 154
EarI CTCTTC 1 cut(s) 154
Ecl136II GAGCTC 1 cut(s) 174
Eco130I CCWWGG 2 cut(s) 386, 408
Eco24I GRGCYC 1 cut(s) 176
Eco47I GGWCC 1 cut(s) 163
Eco53kI GAGCTC 1 cut(s) 174
EcoICRI GAGCTC 1 cut(s) 174
EcoT14I CCWWGG 2 cut(s) 386, 408
EcoT38I GRGCYC 1 cut(s) 176
ErhI CCWWGG 2 cut(s) 386, 408
FaeI CATG 4 cut(s) 117, 312, 365, 412
FalI AAGNNNNNCTT 2 cut(s) 288, 320
FaqI GGGAC 1 cut(s) 149
FatI CATG 4 cut(s) 113, 308, 361, 408
FauNDI CATATG 1 cut(s) 32
Fnu4HI GCNGC 1 cut(s) 396
FokI GGATG 3 cut(s) 104, 254, 425
FriOI GRGCYC 1 cut(s) 176
Fsp4HI GCNGC 1 cut(s) 396
FspBI CTAG 2 cut(s) 387, 465
FspI TGCGCA 1 cut(s) 313
GlaI GCGC 3 cut(s) 313, 360, 490
GluI GCNGC 1 cut(s) 396
HaeIII GGCC 1 cut(s) 108
HhaI GCGC 3 cut(s) 314, 361, 491
Hin1II CATG 4 cut(s) 117, 312, 365, 412
Hin6I GCGC 3 cut(s) 312, 359, 489
HinP1I GCGC 3 cut(s) 312, 359, 489
HinfI GANTC 2 cut(s) 158, 379
HphI GGTGA 1 cut(s) 453
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 3 cut(s) 178, 214, 249
Hpy188III TCNNGA 2 cut(s) 155, 543
Hpy8I GTNNAC 1 cut(s) 79
HpyAV CCTTC 1 cut(s) 128
HpyCH4III ACNGT 2 cut(s) 421, 481
HpyCH4V TGCA 4 cut(s) 72, 308, 398, 532
HpyF10VI GCNNNNNNNGC 7 cut(s) 114, 242, 271, 305, 404, 497, 506
HpyF3I CTNAG 3 cut(s) 66, 168, 196
Hsp92II CATG 4 cut(s) 117, 312, 365, 412
HspAI GCGC 3 cut(s) 312, 359, 489
Kzo9I GATC 2 cut(s) 136, 330
LmnI GCTCC 1 cut(s) 179
LpnPI CCDG 5 cut(s) 58, 122, 180, 224, 360
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 2 cut(s) 82, 232
MaeI CTAG 2 cut(s) 387, 465
MaeIII GTNAC 2 cut(s) 290, 440
MalI GATC 2 cut(s) 138, 332
MboI GATC 2 cut(s) 136, 330
MboII GAAGA 3 cut(s) 47, 141, 450
MhlI GDGCHC 1 cut(s) 176
MlsI TGGCCA 1 cut(s) 108
MluCI AATT 2 cut(s) 338, 369
MluNI TGGCCA 1 cut(s) 108
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 2 cut(s) 47, 483
Mox20I TGGCCA 1 cut(s) 108
MscI TGGCCA 1 cut(s) 108
MseI TTAA 2 cut(s) 62, 270
Msp20I TGGCCA 1 cut(s) 108
MwoI GCNNNNNNNGC 7 cut(s) 114, 242, 271, 305, 404, 497, 506
NcoI CCATGG 1 cut(s) 408
NdeI CATATG 1 cut(s) 32
NdeII GATC 2 cut(s) 136, 330
NlaIII CATG 4 cut(s) 117, 312, 365, 412
NlaIV GGNNCC 1 cut(s) 165
NsbI TGCGCA 1 cut(s) 313
NspI RCATGY 2 cut(s) 117, 312
PaeI GCATGC 1 cut(s) 312
PfeI GAWTC 2 cut(s) 158, 379
PkrI GCNGC 1 cut(s) 397
Psp124BI GAGCTC 1 cut(s) 176
PspN4I GGNNCC 1 cut(s) 165
PspPI GGNCC 1 cut(s) 163
RsaI GTAC 1 cut(s) 222
RsaNI GTAC 1 cut(s) 221
SacI GAGCTC 1 cut(s) 176
SaqAI TTAA 2 cut(s) 62, 270
SatI GCNGC 1 cut(s) 396
Sau3AI GATC 2 cut(s) 136, 330
Sau96I GGNCC 1 cut(s) 163
ScaI AGTACT 1 cut(s) 222
SduI GDGCHC 1 cut(s) 176
SetI ASST 8 cut(s) 84, 176, 202, 267, 283, 397, 466, 470
SfaNI GCATC 2 cut(s) 82, 232
SfcI CTRYAG 1 cut(s) 18
SinI GGWCC 1 cut(s) 163
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
SphI GCATGC 1 cut(s) 312
Sse9I AATT 2 cut(s) 338, 369
SspMI CTAG 2 cut(s) 387, 465
SstI GAGCTC 1 cut(s) 176
StyI CCWWGG 2 cut(s) 386, 408
TaaI ACNGT 2 cut(s) 421, 481
TaqI TCGA 2 cut(s) 287, 348
TaqII GACCGA 1 cut(s) 151
TasI AATT 2 cut(s) 338, 369
TatI WGTACW 1 cut(s) 220
TfiI GAWTC 2 cut(s) 158, 379
Tru1I TTAA 2 cut(s) 62, 270
Tru9I TTAA 2 cut(s) 62, 270
TscAI CASTG 1 cut(s) 74
TseI GCWGC 1 cut(s) 395
TspDTI ATGAA 4 cut(s) 17, 39, 332, 450
TspGWI ACGGA 1 cut(s) 104
TspRI CASTG 1 cut(s) 74
VpaK11BI GGWCC 1 cut(s) 163
XapI RAATTY 1 cut(s) 338
XceI RCATGY 2 cut(s) 117, 312
XmaJI CCTAGG 1 cut(s) 386
XspI CTAG 2 cut(s) 387, 465
ZrmI AGTACT 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.