FvH4_4g27730

PLAC8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016501
Physical Location & Seq
Forward (+)
28588357 .. 28590276
1920 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27730.t1

Sequence Viewer

Length: 474 bp
ATGAGTTCAAACAACTCCGGCCGAAATTCCGATATGGCTCAATGCCAAAAGCAACCTCCCCACCAAGGACAATGGTCCACTGGCCTTTTCGATTGTTTGGAGGATCGTTCGAATTGTATTTTCACTTGCTTTTGTCCATGCATAACCTTTGGGCGAATTGCGGAGATTGTTGATAGAGGAACTACATCGTGTGCGGTGGCCGGCACGATCTACCATTTTCTGGGCGCTGTAGGCTGTGGATGGCTTTATGCTGGCTCCTATCGAACCAAAATGCGAGGACTCTTCTCACTACCGGCAGCGCCATGCCATGATTTTCTGGTTCATAGCTTCTGCTGTGTTTGTTCTCTTTGTCAAGAGTATAGAGAACTCAAAAATCATGGAATTGATCCCACCATAGGGTGGCAAGCCAATGTTGAGAAGTGGAACCGAGAAGGAGTGAAGCCTCCGATGGTCGAACCGGCAATGGCACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

158

Amino Acids

17.44

Weight (kDa)

7.48

Isoelectric Point (pI)

33.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 25 - 122 2.4e-31 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016501)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G58320
fragaria_vesca FvH4_4g27730
prunus_persica Prupe.1G307500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0435591
rosa_laevigata RLG00000006579
rosa_multiflora Rmu_sc0001940.1_g000048 Rmu_sc0006697.1_g000001
rosa_roxburghii Rroxscaffold_5G00376820
rosa_rugosa Rorug04G0286900
rosa_samantha Rh4AG342300 Rh4BG350500 Rh4CG365200 Rh4DG344900
rosa_wichuraiana Rw4G029860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 220, 399
AciI CCGC 2 cut(s) 161, 194
AclWI GGATC 2 cut(s) 111, 380
AcoI YGGCCR 2 cut(s) 19, 198
AcsI RAATTY 1 cut(s) 25
AfiI CCNNNNNNNGG 5 cut(s) 65, 220, 395, 396, 399
AgsI TTSAA 1 cut(s) 9
AluBI AGCT 1 cut(s) 327
AluI AGCT 1 cut(s) 327
AlwI GGATC 2 cut(s) 111, 380
AoxI GGCC 3 cut(s) 19, 82, 198
ApeKI GCWGC 1 cut(s) 296
ApoI RAATTY 1 cut(s) 25
AspLEI GCGC 2 cut(s) 227, 301
AspS9I GGNCC 1 cut(s) 75
AsuII TTCGAA 1 cut(s) 110
AvaII GGWCC 1 cut(s) 75
BbvI GCAGC 1 cut(s) 308
BccI CCATC 2 cut(s) 234, 442
BfmI CTRYAG 1 cut(s) 228
BfoI RGCGCY 2 cut(s) 228, 302
BisI GCNGC 1 cut(s) 297
BlsI GCNGC 1 cut(s) 298
Bme18I GGWCC 1 cut(s) 75
BmgT120I GGNCC 1 cut(s) 75
BmiI GGNNCC 2 cut(s) 256, 425
BoxI GACNNNNGTC 1 cut(s) 73
Bpu14I TTCGAA 1 cut(s) 110
BsaJI CCNNGG 1 cut(s) 64
Bsc4I CCNNNNNNNGG 5 cut(s) 65, 220, 395, 396, 399
Bse118I RCCGGY 3 cut(s) 200, 292, 457
Bse1I ACTGG 1 cut(s) 85
Bse3DI GCAATG 1 cut(s) 468
BseDI CCNNGG 1 cut(s) 64
BseGI GGATG 1 cut(s) 245
BseLI CCNNNNNNNGG 5 cut(s) 65, 220, 395, 396, 399
BseMI GCAATG 1 cut(s) 468
BseNI ACTGG 1 cut(s) 85
BseX3I CGGCCG 1 cut(s) 19
BseXI GCAGC 1 cut(s) 308
Bsh1285I CGRYCG 1 cut(s) 22
BshFI GGCC 3 cut(s) 21, 84, 200
BsiEI CGRYCG 1 cut(s) 22
BsiSI CCGG 4 cut(s) 18, 201, 293, 458
BslI CCNNNNNNNGG 5 cut(s) 65, 220, 395, 396, 399
BsnI GGCC 3 cut(s) 21, 84, 200
Bsp119I TTCGAA 1 cut(s) 110
Bsp143I GATC 3 cut(s) 103, 207, 385
BspACI CCGC 2 cut(s) 161, 194
BspANI GGCC 3 cut(s) 21, 84, 200
BspLI GGNNCC 2 cut(s) 256, 425
BspPI GGATC 2 cut(s) 111, 380
BspT104I TTCGAA 1 cut(s) 110
BsrDI GCAATG 1 cut(s) 468
BsrFI RCCGGY 3 cut(s) 200, 292, 457
BsrI ACTGG 1 cut(s) 85
BssAI RCCGGY 3 cut(s) 200, 292, 457
BssECI CCNNGG 1 cut(s) 64
BssMI GATC 3 cut(s) 103, 207, 385
BssT1I CCWWGG 1 cut(s) 64
Bst6I CTCTTC 1 cut(s) 287
BstBI TTCGAA 1 cut(s) 110
BstC8I GCNNGC 3 cut(s) 202, 253, 405
BstF5I GGATG 1 cut(s) 245
BstH2I RGCGCY 2 cut(s) 228, 302
BstHHI GCGC 2 cut(s) 227, 301
BstKTI GATC 3 cut(s) 106, 210, 388
BstMBI GATC 3 cut(s) 103, 207, 385
BstMCI CGRYCG 1 cut(s) 22
BstMWI GCNNNNNNNGC 1 cut(s) 231
BstPAI GACNNNNGTC 1 cut(s) 73
BstSFI CTRYAG 1 cut(s) 228
BstV1I GCAGC 1 cut(s) 308
BstZI CGGCCG 1 cut(s) 19
BsuRI GGCC 3 cut(s) 21, 84, 200
BtsCI GGATG 1 cut(s) 245
BtsIMutI CAGTG 1 cut(s) 78
Cac8I GCNNGC 3 cut(s) 202, 253, 405
CfoI GCGC 2 cut(s) 227, 301
Cfr10I RCCGGY 3 cut(s) 200, 292, 457
Cfr13I GGNCC 1 cut(s) 75
CviAII CATG 4 cut(s) 138, 303, 308, 377
DpnI GATC 3 cut(s) 105, 209, 387
DpnII GATC 3 cut(s) 103, 207, 385
EaeI YGGCCR 2 cut(s) 19, 198
EagI CGGCCG 1 cut(s) 19
Eam1104I CTCTTC 1 cut(s) 287
EarI CTCTTC 1 cut(s) 287
EclXI CGGCCG 1 cut(s) 19
Eco130I CCWWGG 1 cut(s) 64
Eco47I GGWCC 1 cut(s) 75
Eco52I CGGCCG 1 cut(s) 19
EcoT14I CCWWGG 1 cut(s) 64
EcoT22I ATGCAT 1 cut(s) 143
ErhI CCWWGG 1 cut(s) 64
FaeI CATG 4 cut(s) 141, 306, 311, 380
FatI CATG 4 cut(s) 137, 302, 307, 376
Fnu4HI GCNGC 1 cut(s) 297
FokI GGATG 1 cut(s) 252
Fsp4HI GCNGC 1 cut(s) 297
GlaI GCGC 2 cut(s) 226, 300
GluI GCNGC 1 cut(s) 297
HaeII RGCGCY 2 cut(s) 228, 302
HaeIII GGCC 3 cut(s) 21, 84, 200
HapII CCGG 4 cut(s) 18, 201, 293, 458
HhaI GCGC 2 cut(s) 227, 301
Hin1II CATG 4 cut(s) 141, 306, 311, 380
Hin6I GCGC 2 cut(s) 225, 299
HinP1I GCGC 2 cut(s) 225, 299
HinfI GANTC 1 cut(s) 279
HpaII CCGG 4 cut(s) 18, 201, 293, 458
Hpy166II GTNNAC 1 cut(s) 78
Hpy188I TCNGA 2 cut(s) 31, 447
Hpy188III TCNNGA 1 cut(s) 353
Hpy8I GTNNAC 1 cut(s) 78
HpyAV CCTTC 1 cut(s) 425
HpyCH4IV ACGT 1 cut(s) 469
HpyCH4V TGCA 1 cut(s) 141
HpyF10VI GCNNNNNNNGC 1 cut(s) 231
HpySE526I ACGT 1 cut(s) 469
Hsp92II CATG 4 cut(s) 141, 306, 311, 380
HspAI GCGC 2 cut(s) 225, 299
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
Kzo9I GATC 3 cut(s) 103, 207, 385
LmnI GCTCC 1 cut(s) 260
LpnPI CCDG 7 cut(s) 31, 66, 206, 214, 237, 302, 306
Lsp1109I GCAGC 1 cut(s) 308
MaeII ACGT 1 cut(s) 469
MalI GATC 3 cut(s) 105, 209, 387
MboI GATC 3 cut(s) 103, 207, 385
MboII GAAGA 1 cut(s) 274
MluCI AATT 4 cut(s) 25, 112, 156, 381
MlyI GAGTC 1 cut(s) 273
MnlI CCTC 5 cut(s) 66, 94, 170, 269, 453
Mph1103I ATGCAT 1 cut(s) 143
MroNI GCCGGC 1 cut(s) 200
MseI TTAA 1 cut(s) 472
MspI CCGG 4 cut(s) 18, 201, 293, 458
MwoI GCNNNNNNNGC 1 cut(s) 231
NaeI GCCGGC 1 cut(s) 202
NdeII GATC 3 cut(s) 103, 207, 385
NgoMIV GCCGGC 1 cut(s) 200
NlaIII CATG 4 cut(s) 141, 306, 311, 380
NlaIV GGNNCC 2 cut(s) 256, 425
NsiI ATGCAT 1 cut(s) 143
NspV TTCGAA 1 cut(s) 110
PdiI GCCGGC 1 cut(s) 202
PflMI CCANNNNNTGG 2 cut(s) 220, 399
PkrI GCNGC 1 cut(s) 298
PleI GAGTC 1 cut(s) 273
PpsI GAGTC 1 cut(s) 273
PshAI GACNNNNGTC 1 cut(s) 73
PspN4I GGNNCC 2 cut(s) 256, 425
PspPI GGNCC 1 cut(s) 75
SaqAI TTAA 1 cut(s) 472
SatI GCNGC 1 cut(s) 297
Sau3AI GATC 3 cut(s) 103, 207, 385
Sau96I GGNCC 1 cut(s) 75
SchI GAGTC 1 cut(s) 273
SetI ASST 4 cut(s) 58, 149, 329, 472
SfcI CTRYAG 1 cut(s) 228
SfuI TTCGAA 1 cut(s) 110
SinI GGWCC 1 cut(s) 75
Sse9I AATT 4 cut(s) 25, 112, 156, 381
SsiI CCGC 2 cut(s) 161, 194
StyI CCWWGG 1 cut(s) 64
TaiI ACGT 1 cut(s) 472
TaqI TCGA 4 cut(s) 90, 110, 262, 453
TasI AATT 4 cut(s) 25, 112, 156, 381
Tru1I TTAA 1 cut(s) 472
Tru9I TTAA 1 cut(s) 472
TscAI CASTG 1 cut(s) 85
TseI GCWGC 1 cut(s) 296
TspDTI ATGAA 1 cut(s) 311
TspRI CASTG 1 cut(s) 85
Van91I CCANNNNNTGG 2 cut(s) 220, 399
VpaK11BI GGWCC 1 cut(s) 75
XapI RAATTY 1 cut(s) 25
Zsp2I ATGCAT 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.