FvH4_4g30420

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011334
Physical Location & Seq
Forward (+)
30054497 .. 30057146
2650 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30420.t1

Sequence Viewer

Length: 990 bp
ATGGCGCTTCGTCTCCGCTTCGCCGCATTCTCTCTCCTCTTCTTCCTCCTCCTCTCTCCTCTTCTCCTCCACGCCAAAACCCTAAAACGCGACATGAAAGCGTTGAACGAGATCAAGGCCTCGCTGGGGTGGAGAGTAGTCTACGCCTGGGTCGGAGACGATCCGTGCGGCGACGGCGACCTTCCGCCGTGGTCCGGCGTCACTTGCTCCACTCAAGGCGATTACCGAGTCGTCACTGAGCTGGAGGTTTATGCTGTCTCGATTGTTGGGCCTTTTCCTACTGCCGTCACTAATCTCTTGGATCTCACTAGGCTAGATCTCCATAACAACAAGCTAACGGGGCCTATCCCGCCTCAGATTGGACGATTGAAGCGTCTCCGAATACTTAACTTGAGATGGAATAAGCTGCAAGATGTCATTCCTCCTGAGATTGGTGAACTGAAGAGTTTAACCCATTTGTATCTAAGCTTCAACAGTTTCAAAGGGGAAATTCCTAGAGAGCTTGCTAATCTTCCTGCTCTTCGCTATCTCTACCTGCAAGAAAATCGTCTTATTGGGCGAATTCCACCAGAACTAGGAACTCTGCCAAATCTTCGGCACTTGGATGTTGGCAACAATCATTTGGTGGGTACTATTAGGGAACTTATACGCATTGAGGGCTGCTTTCCTGCTTTGCGTAACCTTTACCTGAACAACAATTATCTTACGGGAGGAATTCCAGCTCAGCTTGCCAACTTGACCAACTTGGAAATCTTGTACCTGTCTTACAACAAGATGTCTGGGATTGTTCCATTAGCAATATCCCATATTCCTCGATTGACTTACTTGTACTTGGATCACAATCAGTTCTCAGGAAGAATTCCTGATGCCTTCTATAAACACCCATTCTTGAAAGACATGTATATTGAAGGGAATGCATTCAAGCCAGGTGTAAAACCGATAGGCATACACAAAGTTCTTGAGCTCACTGACACGGAATTCCTCGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8

Protein Analysis

330

Amino Acids

37.24

Weight (kDa)

9.11

Isoelectric Point (pI)

39.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 96 - 204 4.1e-10 Leucine-rich repeat region
LRR_8 PF13855 125 - 184 1.1e-09 Leucine rich repeat
LRR_4 PF12799 172 - 215 1.5e-07 Leucine Rich repeats (2 copies)
LRR_8 PF13855 223 - 283 4.3e-14 Leucine rich repeat
LRR_4 PF12799 223 - 259 3.3e-07 Leucine Rich repeats (2 copies)
LRR_4 PF12799 247 - 282 1.6e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011334)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61240 AT5G61240
fragaria_vesca FvH4_4g30420
malus_domestica MD13G1065200.v1.1 MD16G1063500.v1.1
prunus_persica Prupe.1G283000_v2.0.a1 Prupe.1G283000_v2.0.a1
pyrus_communis pycom13g05760 pycom16g05560
rosa_chinensis RchiOBHm_Chr4g0438711
rosa_laevigata RLG00000006335
rosa_multiflora Rmu_sc0002505.1_g000005
rosa_roxburghii Rroxscaffold_5G00379750
rosa_rugosa Rorug04G0312700
rosa_samantha Rh4AG363300 Rh4BG374700 Rh4CG388900 Rh4DG369200
rosa_wichuraiana Rw0G007180 Rw0G016730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 543
AccI GTMKAC 1 cut(s) 141
AccII CGCG 1 cut(s) 90
AciI CCGC 5 cut(s) 16, 24, 168, 185, 350
AclWI GGATC 3 cut(s) 155, 309, 843
AcsI RAATTY 5 cut(s) 489, 561, 714, 858, 977
AcuI CTGAAG 1 cut(s) 461
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 3 cut(s) 631, 758, 830
AfiI CCNNNNNNNGG 5 cut(s) 126, 194, 359, 431, 575
AflIII ACRYGT 1 cut(s) 897
AgsI TTSAA 7 cut(s) 106, 370, 472, 481, 892, 908, 922
AjnI CCWGG 2 cut(s) 146, 925
AluBI AGCT 8 cut(s) 241, 334, 406, 468, 502, 722, 727, 964
AluI AGCT 8 cut(s) 241, 334, 406, 468, 502, 722, 727, 964
Alw21I GWGCWC 1 cut(s) 966
Alw26I GTCTC 4 cut(s) 17, 150, 262, 380
AlwI GGATC 3 cut(s) 155, 309, 843
AoxI GGCC 3 cut(s) 117, 269, 341
ApeKI GCWGC 2 cut(s) 406, 660
ApoI RAATTY 5 cut(s) 489, 561, 714, 858, 977
Asp700I GAANNNNTTC 1 cut(s) 917
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 3 cut(s) 192, 269, 341
AsuHPI GGTGA 1 cut(s) 446
AvaII GGWCC 1 cut(s) 192
BanII GRGCYC 1 cut(s) 966
BarI GAAGNNNNNNTAC 2 cut(s) 452, 484
Bbv12I GWGCWC 1 cut(s) 966
BbvI GCAGC 2 cut(s) 393, 647
BccI CCATC 1 cut(s) 390
BceAI ACGGC 3 cut(s) 172, 190, 269
BcgI CGANNNNNNTGC 2 cut(s) 527, 561
BciT130I CCWGG 2 cut(s) 148, 927
BcoDI GTCTC 4 cut(s) 17, 150, 262, 380
BfaI CTAG 5 cut(s) 309, 314, 495, 575, 988
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 543
BglII AGATCT 1 cut(s) 316
BisI GCNGC 4 cut(s) 24, 169, 407, 661
BlpI GCTNAGC 1 cut(s) 723
BlsI GCNGC 4 cut(s) 25, 170, 408, 662
Bme1390I CCNGG 2 cut(s) 148, 927
Bme18I GGWCC 1 cut(s) 192
BmgT120I GGNCC 3 cut(s) 192, 269, 341
BmiI GGNNCC 1 cut(s) 342
BmrFI CCNGG 2 cut(s) 148, 927
BmsI GCATC 1 cut(s) 856
BpmI CTGGAG 1 cut(s) 263
Bpu1102I GCTNAGC 1 cut(s) 723
BpuEI CTTGAG 3 cut(s) 198, 412, 980
BsaBI GATNNNNATC 1 cut(s) 840
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 2 cut(s) 147, 188
Bsc4I CCNNNNNNNGG 5 cut(s) 126, 194, 359, 431, 575
Bse8I GATNNNNATC 1 cut(s) 840
BseBI CCWGG 2 cut(s) 148, 927
BseDI CCNNGG 2 cut(s) 147, 188
BseGI GGATG 1 cut(s) 610
BseJI GATNNNNATC 1 cut(s) 840
BseLI CCNNNNNNNGG 5 cut(s) 126, 194, 359, 431, 575
BseMII CTCAG 5 cut(s) 228, 368, 417, 737, 864
BseRI GAGGAG 5 cut(s) 26, 38, 41, 48, 56
BseXI GCAGC 2 cut(s) 393, 647
BseYI CCCAGC 1 cut(s) 124
Bsh1236I CGCG 1 cut(s) 90
BshFI GGCC 3 cut(s) 119, 271, 343
BsiHKAI GWGCWC 1 cut(s) 966
BsiSI CCGG 1 cut(s) 195
BslI CCNNNNNNNGG 5 cut(s) 126, 194, 359, 431, 575
BsmAI GTCTC 4 cut(s) 17, 150, 262, 380
BsmBI CGTCTC 3 cut(s) 17, 150, 380
BsmI GAATGC 3 cut(s) 26, 917, 919
BsnI GGCC 3 cut(s) 119, 271, 343
Bsp1286I GDGCHC 1 cut(s) 966
Bsp143I GATC 5 cut(s) 111, 160, 301, 316, 835
Bsp1720I GCTNAGC 1 cut(s) 723
BspACI CCGC 5 cut(s) 16, 24, 168, 185, 350
BspANI GGCC 3 cut(s) 119, 271, 343
BspCNI CTCAG 5 cut(s) 229, 367, 418, 736, 863
BspFNI CGCG 1 cut(s) 90
BspLI GGNNCC 1 cut(s) 342
BspMI ACCTGC 1 cut(s) 543
BspPI GGATC 3 cut(s) 155, 309, 843
BspQI GCTCTTC 1 cut(s) 525
BssECI CCNNGG 2 cut(s) 147, 188
BssMI GATC 5 cut(s) 111, 160, 301, 316, 835
BssNI GRCGYC 1 cut(s) 198
Bst2UI CCWGG 2 cut(s) 148, 927
Bst4CI ACNGT 1 cut(s) 476
Bst6I CTCTTC 4 cut(s) 44, 66, 437, 525
BstACI GRCGYC 1 cut(s) 198
BstC8I GCNNGC 2 cut(s) 504, 729
BstDEI CTNAG 6 cut(s) 237, 354, 426, 464, 723, 850
BstDSI CCRYGG 1 cut(s) 188
BstF5I GGATG 1 cut(s) 610
BstFNI CGCG 1 cut(s) 90
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 5 cut(s) 114, 163, 304, 319, 838
BstMAI GTCTC 4 cut(s) 17, 150, 262, 380
BstMBI GATC 5 cut(s) 111, 160, 301, 316, 835
BstMWI GCNNNNNNNGC 6 cut(s) 174, 204, 340, 349, 657, 728
BstNI CCWGG 2 cut(s) 148, 927
BstNSI RCATGY 1 cut(s) 901
BstSCI CCNGG 2 cut(s) 146, 925
BstUI CGCG 1 cut(s) 90
BstV1I GCAGC 2 cut(s) 393, 647
BstX2I RGATCY 2 cut(s) 301, 316
BstYI RGATCY 2 cut(s) 301, 316
BsuRI GGCC 3 cut(s) 119, 271, 343
BtgI CCRYGG 1 cut(s) 188
BtsCI GGATG 1 cut(s) 610
BtsIMutI CAGTG 2 cut(s) 234, 966
BveI ACCTGC 1 cut(s) 543
Cac8I GCNNGC 2 cut(s) 504, 729
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 3 cut(s) 192, 269, 341
CseI GACGC 2 cut(s) 187, 362
Csp6I GTAC 3 cut(s) 630, 757, 829
CviAII CATG 2 cut(s) 94, 898
CviQI GTAC 3 cut(s) 630, 757, 829
DdeI CTNAG 6 cut(s) 237, 354, 426, 464, 723, 850
DpnI GATC 5 cut(s) 113, 162, 303, 318, 837
DpnII GATC 5 cut(s) 111, 160, 301, 316, 835
Eam1104I CTCTTC 4 cut(s) 44, 66, 437, 525
EarI CTCTTC 4 cut(s) 44, 66, 437, 525
EciI GGCGGA 1 cut(s) 174
Ecl136II GAGCTC 1 cut(s) 964
Eco147I AGGCCT 1 cut(s) 119
Eco24I GRGCYC 1 cut(s) 966
Eco47I GGWCC 1 cut(s) 192
Eco53kI GAGCTC 1 cut(s) 964
Eco57I CTGAAG 1 cut(s) 461
EcoICRI GAGCTC 1 cut(s) 964
EcoO109I RGGNCCY 1 cut(s) 341
EcoRI GAATTC 4 cut(s) 561, 714, 858, 977
EcoRII CCWGG 2 cut(s) 146, 925
EcoT22I ATGCAT 1 cut(s) 919
EcoT38I GRGCYC 1 cut(s) 966
Esp3I CGTCTC 3 cut(s) 17, 150, 380
FaeI CATG 2 cut(s) 97, 901
FaiI YATR 9 cut(s) 95, 252, 324, 647, 807, 876, 899, 903, 947
FatI CATG 2 cut(s) 93, 897
FauI CCCGC 1 cut(s) 357
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 4 cut(s) 24, 169, 407, 661
FokI GGATG 1 cut(s) 617
FriOI GRGCYC 1 cut(s) 966
Fsp4HI GCNGC 4 cut(s) 24, 169, 407, 661
FspBI CTAG 5 cut(s) 309, 314, 495, 575, 988
GlaI GCGC 1 cut(s) 6
GluI GCNGC 4 cut(s) 24, 169, 407, 661
GsaI CCCAGC 1 cut(s) 128
GsuI CTGGAG 1 cut(s) 263
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 3 cut(s) 119, 271, 343
HapII CCGG 1 cut(s) 195
HgaI GACGC 2 cut(s) 187, 362
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 2 cut(s) 97, 901
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HindIII AAGCTT 1 cut(s) 466
HinfI GANTC 1 cut(s) 228
HpaII CCGG 1 cut(s) 195
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 2 cut(s) 142, 437
Hpy188I TCNGA 3 cut(s) 155, 357, 380
Hpy188III TCNNGA 6 cut(s) 259, 425, 852, 863, 889, 959
Hpy8I GTNNAC 2 cut(s) 142, 437
Hpy99I CGWCG 1 cut(s) 176
HpyAV CCTTC 3 cut(s) 191, 880, 902
HpyCH4III ACNGT 1 cut(s) 476
HpyCH4V TGCA 3 cut(s) 409, 538, 917
HpyF10VI GCNNNNNNNGC 6 cut(s) 174, 204, 340, 349, 657, 728
HpyF3I CTNAG 6 cut(s) 237, 354, 426, 464, 723, 850
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 2 cut(s) 97, 901
HspAI GCGC 1 cut(s) 5
Kzo9I GATC 5 cut(s) 111, 160, 301, 316, 835
LguI GCTCTTC 1 cut(s) 525
LmnI GCTCC 1 cut(s) 212
Lsp1109I GCAGC 2 cut(s) 393, 647
LweI GCATC 1 cut(s) 856
MaeI CTAG 5 cut(s) 309, 314, 495, 575, 988
MaeIII GTNAC 4 cut(s) 199, 232, 286, 677
MalI GATC 5 cut(s) 113, 162, 303, 318, 837
MboI GATC 5 cut(s) 111, 160, 301, 316, 835
MboII GAAGA 8 cut(s) 31, 34, 53, 454, 503, 512, 584, 867
MflI RGATCY 2 cut(s) 301, 316
MhlI GDGCHC 1 cut(s) 966
MluCI AATT 6 cut(s) 489, 561, 697, 714, 858, 977
MlyI GAGTC 1 cut(s) 237
MmeI TCCRAC 1 cut(s) 133
Mph1103I ATGCAT 1 cut(s) 919
MroXI GAANNNNTTC 1 cut(s) 917
MseI TTAA 2 cut(s) 387, 449
MslI CAYNNNNRTG 1 cut(s) 603
MspI CCGG 1 cut(s) 195
MspR9I CCNGG 2 cut(s) 148, 927
Mva1269I GAATGC 3 cut(s) 26, 917, 919
MvaI CCWGG 2 cut(s) 148, 927
MvnI CGCG 1 cut(s) 90
MwoI GCNNNNNNNGC 6 cut(s) 174, 204, 340, 349, 657, 728
NdeII GATC 5 cut(s) 111, 160, 301, 316, 835
NlaIII CATG 2 cut(s) 97, 901
NlaIV GGNNCC 1 cut(s) 342
NmuCI GTSAC 3 cut(s) 199, 232, 286
NsiI ATGCAT 1 cut(s) 919
NspI RCATGY 1 cut(s) 901
PceI AGGCCT 1 cut(s) 119
PciI ACATGT 1 cut(s) 897
PciSI GCTCTTC 1 cut(s) 525
PcsI WCGNNNNNNNCGW 1 cut(s) 370
PctI GAATGC 3 cut(s) 26, 917, 919
PdmI GAANNNNTTC 1 cut(s) 917
PkrI GCNGC 4 cut(s) 25, 170, 408, 662
PleI GAGTC 1 cut(s) 236
PpsI GAGTC 1 cut(s) 236
PscI ACATGT 1 cut(s) 897
Psp124BI GAGCTC 1 cut(s) 966
Psp6I CCWGG 2 cut(s) 146, 925
PspFI CCCAGC 1 cut(s) 124
PspGI CCWGG 2 cut(s) 146, 925
PspN4I GGNNCC 1 cut(s) 342
PspPI GGNCC 3 cut(s) 192, 269, 341
PsuI RGATCY 2 cut(s) 301, 316
RsaI GTAC 3 cut(s) 631, 758, 830
RsaNI GTAC 3 cut(s) 630, 757, 829
RseI CAYNNNNRTG 1 cut(s) 603
SacI GAGCTC 1 cut(s) 966
SapI GCTCTTC 1 cut(s) 525
SaqAI TTAA 2 cut(s) 387, 449
SatI GCNGC 4 cut(s) 24, 169, 407, 661
Sau3AI GATC 5 cut(s) 111, 160, 301, 316, 835
Sau96I GGNCC 3 cut(s) 192, 269, 341
SchI GAGTC 1 cut(s) 237
ScrFI CCNGG 2 cut(s) 148, 927
SduI GDGCHC 1 cut(s) 966
SfaNI GCATC 1 cut(s) 856
SinI GGWCC 1 cut(s) 192
SmiMI CAYNNNNRTG 1 cut(s) 603
SmlI CTYRAG 3 cut(s) 213, 391, 959
SmoI CTYRAG 3 cut(s) 213, 391, 959
Sse9I AATT 6 cut(s) 489, 561, 697, 714, 858, 977
SseBI AGGCCT 1 cut(s) 119
SsiI CCGC 5 cut(s) 16, 24, 168, 185, 350
SspMI CTAG 5 cut(s) 309, 314, 495, 575, 988
SstI GAGCTC 1 cut(s) 966
StuI AGGCCT 1 cut(s) 119
StyD4I CCNGG 2 cut(s) 146, 925
TaaI ACNGT 1 cut(s) 476
TaqI TCGA 2 cut(s) 260, 814
TasI AATT 6 cut(s) 489, 561, 697, 714, 858, 977
TatI WGTACW 1 cut(s) 828
TauI GCSGC 2 cut(s) 26, 171
Tru1I TTAA 2 cut(s) 387, 449
Tru9I TTAA 2 cut(s) 387, 449
TscAI CASTG 2 cut(s) 241, 973
TseFI GTSAC 3 cut(s) 199, 232, 286
TseI GCWGC 2 cut(s) 406, 660
Tsp45I GTSAC 3 cut(s) 199, 232, 286
TspDTI ATGAA 1 cut(s) 110
TspGWI ACGGA 2 cut(s) 153, 989
TspRI CASTG 2 cut(s) 241, 973
VpaK11BI GGWCC 1 cut(s) 192
XapI RAATTY 5 cut(s) 489, 561, 714, 858, 977
XceI RCATGY 1 cut(s) 901
XmiI GTMKAC 1 cut(s) 141
XmnI GAANNNNTTC 1 cut(s) 917
XspI CTAG 5 cut(s) 309, 314, 495, 575, 988
Zsp2I ATGCAT 1 cut(s) 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.