FvH4_4g31200

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016836
Physical Location & Seq
Reverse (-)
30453329 .. 30453853
525 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31200.t1

Sequence Viewer

Length: 525 bp
ATGGAGGACATACTAGCAGCCGCAGCTCAACCGGGCTCCCTCTATGTTCTTAGATTGTTCCAACCCTTGTTGAAGCTTTGGACCAAAACCCACAATTACCTAAATTATTCTGACCCTCATGTACTACAACCAACCCTTGATCAGCCTCTGCAATGCAACAAAGGACAAGATGAACCGTTGCTGGGAAAAGAAGAAGTGAAGATGGTGATGGAAAGGCTCGGACTATGCTACCAAGATGAGGGAGATGATGTTATTACTAATGAAGACATGGTTGGAGCAGAGGAGGTTTCAAGAGTGTTTAGTGAGGCGGAGCCAAGCTTGGAGGAGGTGAAGGAAGCCTTTGATGTGTTTGATGAGAACAGAGATGGGTTTATAGACGCAGCTGATTTGCAGAGAGTTTTGTGCAATCTGGGTTTGAAGGGGAAGTTTGGATTGGAGAAATGCCGGGGGATGATCAAGGCAGTAGACATGAACCAAGATGGACTGGTTGATTTTGAAGAGTTCGTCGAACTCATGGACAACTAG

Protein Analysis

175

Amino Acids

19.69

Weight (kDa)

4.27

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 108 - 172 5.6e-13 EF-hand domain pair
EF-hand_1 PF00036 109 - 134 4.8e-06 EF hand domain
EF-hand_6 PF13405 109 - 138 2.6e-07 EF-hand domain
EF-hand_5 PF13202 111 - 134 8.5e-06 EF hand
EF-hand_8 PF13833 123 - 172 2.9e-09 EF-hand domain pair
EF-hand_1 PF00036 148 - 172 1e-05 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016836)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31200
malus_domestica MD13G1070500.v1.1
prunus_persica Prupe.1G276100_v2.0.a1
pyrus_communis pycom13g06330
rosa_chinensis RchiOBHm_Chr4g0439581
rosa_laevigata RLG00000006256
rosa_multiflora Rmu_sc0002549.1_g000024
rosa_roxburghii Rroxscaffold_5G00380520
rosa_rugosa Rorug04G0320000
rosa_samantha Rh4AG371000 Rh4BG382800 Rh4CG397400 Rh4DG377600
rosa_wichuraiana Rw4G031630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 465
AciI CCGC 2 cut(s) 21, 308
AfaI GTAC 1 cut(s) 123
AfiI CCNNNNNNNGG 2 cut(s) 182, 238
AgsI TTSAA 4 cut(s) 73, 291, 418, 497
AjuI GAANNNNNNNTTGG 4 cut(s) 255, 287, 416, 448
AluBI AGCT 4 cut(s) 26, 76, 318, 383
AluI AGCT 4 cut(s) 26, 76, 318, 383
AlwNI CAGNNNCTG 1 cut(s) 148
ApeKI GCWGC 3 cut(s) 17, 23, 380
AspS9I GGNCC 1 cut(s) 81
AsuC2I CCSGG 2 cut(s) 33, 446
AsuHPI GGTGA 2 cut(s) 217, 340
AvaII GGWCC 1 cut(s) 81
BanII GRGCYC 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 270
BbvI GCAGC 3 cut(s) 29, 35, 392
BccI CCATC 4 cut(s) 196, 202, 359, 473
BclI TGATCA 2 cut(s) 139, 453
BcnI CCSGG 2 cut(s) 33, 446
BfaI CTAG 2 cut(s) 14, 523
BisI GCNGC 4 cut(s) 18, 21, 24, 381
BlsI GCNGC 4 cut(s) 19, 22, 25, 382
Bme1390I CCNGG 2 cut(s) 33, 446
Bme18I GGWCC 1 cut(s) 81
BmgT120I GGNCC 1 cut(s) 81
BmiI GGNNCC 2 cut(s) 37, 312
BmrFI CCNGG 2 cut(s) 33, 446
BpiI GAAGAC 1 cut(s) 270
BpuMI CCSGG 2 cut(s) 33, 446
BsaJI CCNNGG 1 cut(s) 445
BsaXI ACNNNNNCTCC 2 cut(s) 234, 264
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 238
Bse1I ACTGG 1 cut(s) 489
Bse3DI GCAATG 1 cut(s) 158
BseDI CCNNGG 1 cut(s) 445
BseGI GGATG 1 cut(s) 456
BseLI CCNNNNNNNGG 2 cut(s) 182, 238
BseMI GCAATG 1 cut(s) 158
BseNI ACTGG 1 cut(s) 489
BseRI GAGGAG 2 cut(s) 296, 338
BseXI GCAGC 3 cut(s) 29, 35, 392
BseYI CCCAGC 1 cut(s) 181
BsiSI CCGG 2 cut(s) 32, 445
BslI CCNNNNNNNGG 2 cut(s) 182, 238
Bsp1286I GDGCHC 1 cut(s) 38
Bsp143I GATC 2 cut(s) 139, 453
BspACI CCGC 2 cut(s) 21, 308
BspLI GGNNCC 2 cut(s) 37, 312
BsrDI GCAATG 1 cut(s) 158
BsrI ACTGG 1 cut(s) 489
BssECI CCNNGG 1 cut(s) 445
BssMI GATC 2 cut(s) 139, 453
Bst4CI ACNGT 1 cut(s) 177
Bst6I CTCTTC 1 cut(s) 492
BstDEI CTNAG 1 cut(s) 50
BstF5I GGATG 1 cut(s) 456
BstKTI GATC 2 cut(s) 142, 456
BstMBI GATC 2 cut(s) 139, 453
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstSCI CCNGG 2 cut(s) 31, 444
BstV1I GCAGC 3 cut(s) 29, 35, 392
BstV2I GAAGAC 1 cut(s) 270
BtsCI GGATG 1 cut(s) 456
CaiI CAGNNNCTG 1 cut(s) 148
Cfr13I GGNCC 1 cut(s) 81
CseI GACGC 1 cut(s) 386
Csp6I GTAC 1 cut(s) 122
CviAII CATG 4 cut(s) 119, 268, 469, 514
CviQI GTAC 1 cut(s) 122
DdeI CTNAG 1 cut(s) 50
DpnI GATC 2 cut(s) 141, 455
DpnII GATC 2 cut(s) 139, 453
Eam1104I CTCTTC 1 cut(s) 492
EarI CTCTTC 1 cut(s) 492
EciI GGCGGA 1 cut(s) 323
Eco24I GRGCYC 1 cut(s) 38
Eco47I GGWCC 1 cut(s) 81
EcoT38I GRGCYC 1 cut(s) 38
FaeI CATG 4 cut(s) 122, 271, 472, 517
FaiI YATR 8 cut(s) 11, 45, 120, 226, 269, 374, 470, 515
FalI AAGNNNNNCTT 2 cut(s) 323, 355
FatI CATG 4 cut(s) 118, 267, 468, 513
FbaI TGATCA 2 cut(s) 139, 453
FblI GTMKAC 1 cut(s) 465
Fnu4HI GCNGC 4 cut(s) 18, 21, 24, 381
FokI GGATG 1 cut(s) 463
FriOI GRGCYC 1 cut(s) 38
Fsp4HI GCNGC 4 cut(s) 18, 21, 24, 381
FspBI CTAG 2 cut(s) 14, 523
GluI GCNGC 4 cut(s) 18, 21, 24, 381
GsaI CCCAGC 1 cut(s) 185
HapII CCGG 2 cut(s) 32, 445
HgaI GACGC 1 cut(s) 386
Hin1II CATG 4 cut(s) 122, 271, 472, 517
HindIII AAGCTT 2 cut(s) 74, 316
HpaII CCGG 2 cut(s) 32, 445
HphI GGTGA 2 cut(s) 217, 340
Hpy166II GTNNAC 1 cut(s) 466
Hpy188I TCNGA 2 cut(s) 112, 221
Hpy188III TCNNGA 1 cut(s) 291
Hpy8I GTNNAC 1 cut(s) 466
Hpy99I CGWCG 1 cut(s) 509
HpyAV CCTTC 2 cut(s) 325, 412
HpyCH4III ACNGT 1 cut(s) 177
HpyCH4V TGCA 4 cut(s) 151, 156, 391, 405
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpyF3I CTNAG 1 cut(s) 50
Hsp92II CATG 4 cut(s) 122, 271, 472, 517
Ksp22I TGATCA 2 cut(s) 139, 453
Kzo9I GATC 2 cut(s) 139, 453
LmnI GCTCC 3 cut(s) 41, 275, 310
LpnPI CCDG 5 cut(s) 45, 167, 395, 458, 470
Lsp1109I GCAGC 3 cut(s) 29, 35, 392
MaeI CTAG 2 cut(s) 14, 523
MalI GATC 2 cut(s) 141, 455
MboI GATC 2 cut(s) 139, 453
MboII GAAGA 4 cut(s) 203, 211, 275, 509
MhlI GDGCHC 1 cut(s) 38
MluCI AATT 2 cut(s) 94, 103
MmeI TCCRAC 2 cut(s) 85, 253
MnlI CCTC 9 cut(s) 50, 126, 156, 232, 274, 277, 298, 316, 319
MspA1I CMGCKG 1 cut(s) 383
MspI CCGG 2 cut(s) 32, 445
MspR9I CCNGG 2 cut(s) 33, 446
MwoI GCNNNNNNNGC 1 cut(s) 23
NciI CCSGG 2 cut(s) 33, 446
NdeII GATC 2 cut(s) 139, 453
NlaIII CATG 4 cut(s) 122, 271, 472, 517
NlaIV GGNNCC 2 cut(s) 37, 312
PkrI GCNGC 4 cut(s) 19, 22, 25, 382
PspFI CCCAGC 1 cut(s) 181
PspN4I GGNNCC 2 cut(s) 37, 312
PspPI GGNCC 1 cut(s) 81
PstNI CAGNNNCTG 1 cut(s) 148
PvuII CAGCTG 1 cut(s) 383
RsaI GTAC 1 cut(s) 123
RsaNI GTAC 1 cut(s) 122
SatI GCNGC 4 cut(s) 18, 21, 24, 381
Sau3AI GATC 2 cut(s) 139, 453
Sau96I GGNCC 1 cut(s) 81
ScrFI CCNGG 2 cut(s) 33, 446
SduI GDGCHC 1 cut(s) 38
SetI ASST 7 cut(s) 28, 78, 102, 288, 320, 330, 385
SinI GGWCC 1 cut(s) 81
Sse9I AATT 2 cut(s) 94, 103
SsiI CCGC 2 cut(s) 21, 308
SspMI CTAG 2 cut(s) 14, 523
StyD4I CCNGG 2 cut(s) 31, 444
TaaI ACNGT 1 cut(s) 177
TaqI TCGA 1 cut(s) 507
TasI AATT 2 cut(s) 94, 103
TatI WGTACW 1 cut(s) 121
TauI GCSGC 1 cut(s) 23
TseI GCWGC 3 cut(s) 17, 23, 380
TspDTI ATGAA 3 cut(s) 186, 276, 485
VpaK11BI GGWCC 1 cut(s) 81
XmiI GTMKAC 1 cut(s) 465
XspI CTAG 2 cut(s) 14, 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.