MD13G1070500.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016836
Physical Location & Seq
Reverse (-)
4965290 .. 4965823
534 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1070500.v1.1.491

Sequence Viewer

Length: 534 bp
ATGAAGGACATACCAACAAACACAACTCAACTGGTCTGCCTTTATTTTTCAGCATTGATTTCTTGGGTATTAAATTTCTTAGTTTTGTCTTTACACCACATGTTTCATCCCCTTGTAATATTTTTCTCCACACCTTCGAAGAGGGTTTGCACCAATACCCTCAAATCAACTTCTGATAAAACAGAAGTAACGGAAGAAGTGAAGACGGTGATGGACAGGCTAGGGTTAACATGTTATGATGCACAAGGAGATGATATTCAAGGCGGGGTTGGTGCGGAGGAGGAGCTTTCGAGACTGTTCGATGAGGAGGAGCCTAGCTTGGAGGAGGTAAAGGAAGCGTTTGATGTGTTTGATGAAAATAAAGACGGGTTTATAGATGCTGCAGAGTTACAAAGAGTTTTATACATTTTGGGTTTGGAGGACGGGTTTGGATTGGACGAATGCCGGAGGATGATCGAGGCCGTTGATACGAACGGAGATGGACTAATTGATTTTGATGAGTTTGTGAAACATATTGAGGACAGCTTTTGTTGA

Protein Analysis

178

Amino Acids

20.03

Weight (kDa)

4.24

Isoelectric Point (pI)

45.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 108 - 171 1.8e-14 EF-hand domain pair
EF-hand_1 PF00036 109 - 134 3.6e-07 EF hand domain
EF-hand_6 PF13405 109 - 138 2.6e-07 EF-hand domain
EF-hand_5 PF13202 111 - 134 3.5e-07 EF hand
EF-hand_8 PF13833 122 - 169 3.3e-08 EF-hand domain pair
EF-hand_1 PF00036 147 - 170 5.8e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016836)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31200
malus_domestica MD13G1070500.v1.1
prunus_persica Prupe.1G276100_v2.0.a1
pyrus_communis pycom13g06330
rosa_chinensis RchiOBHm_Chr4g0439581
rosa_laevigata RLG00000006256
rosa_multiflora Rmu_sc0002549.1_g000024
rosa_roxburghii Rroxscaffold_5G00380520
rosa_rugosa Rorug04G0320000
rosa_samantha Rh4AG371000 Rh4BG382800 Rh4CG397400 Rh4DG377600
rosa_wichuraiana Rw4G031630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 264, 275
AcsI RAATTY 1 cut(s) 73
AflIII ACRYGT 2 cut(s) 99, 230
AgsI TTSAA 1 cut(s) 260
AluBI AGCT 3 cut(s) 286, 318, 525
AluI AGCT 3 cut(s) 286, 318, 525
Alw26I GTCTC 1 cut(s) 286
AoxI GGCC 1 cut(s) 459
ApeKI GCWGC 1 cut(s) 380
ApoI RAATTY 1 cut(s) 73
AsuHPI GGTGA 1 cut(s) 220
AsuII TTCGAA 1 cut(s) 137
BbsI GAAGAC 1 cut(s) 209
BbvI GCAGC 1 cut(s) 367
BccI CCATC 2 cut(s) 205, 473
BceAI ACGGC 1 cut(s) 446
BcoDI GTCTC 1 cut(s) 286
BfaI CTAG 2 cut(s) 221, 315
BfmI CTRYAG 1 cut(s) 381
BisI GCNGC 1 cut(s) 381
BlsI GCNGC 1 cut(s) 382
BmiI GGNNCC 1 cut(s) 312
BmsI GCATC 2 cut(s) 229, 367
BpiI GAAGAC 1 cut(s) 209
Bpu14I TTCGAA 1 cut(s) 137
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
Bse1I ACTGG 1 cut(s) 36
BseGI GGATG 2 cut(s) 106, 456
BseNI ACTGG 1 cut(s) 36
BseRI GAGGAG 5 cut(s) 293, 296, 320, 323, 338
BseXI GCAGC 1 cut(s) 367
BshFI GGCC 1 cut(s) 461
BsiSI CCGG 1 cut(s) 445
BsmAI GTCTC 1 cut(s) 286
BsmI GAATGC 1 cut(s) 446
BsnI GGCC 1 cut(s) 461
Bsp119I TTCGAA 1 cut(s) 137
Bsp143I GATC 1 cut(s) 453
BspACI CCGC 2 cut(s) 264, 275
BspANI GGCC 1 cut(s) 461
BspLI GGNNCC 1 cut(s) 312
BspMAI CTGCAG 1 cut(s) 385
BspT104I TTCGAA 1 cut(s) 137
BsrI ACTGG 1 cut(s) 36
BssMI GATC 1 cut(s) 453
Bst4CI ACNGT 2 cut(s) 208, 297
Bst6I CTCTTC 1 cut(s) 134
BstBI TTCGAA 1 cut(s) 137
BstDEI CTNAG 1 cut(s) 79
BstF5I GGATG 2 cut(s) 106, 456
BstKTI GATC 1 cut(s) 456
BstMAI GTCTC 1 cut(s) 286
BstMBI GATC 1 cut(s) 453
BstNSI RCATGY 2 cut(s) 103, 234
BstSFI CTRYAG 1 cut(s) 381
BstV1I GCAGC 1 cut(s) 367
BstV2I GAAGAC 1 cut(s) 209
BsuRI GGCC 1 cut(s) 461
BtsCI GGATG 2 cut(s) 106, 456
CviAII CATG 2 cut(s) 100, 231
CviJI RGCY 6 cut(s) 220, 286, 313, 318, 461, 525
CviKI_1 RGCY 6 cut(s) 220, 286, 313, 318, 461, 525
DdeI CTNAG 1 cut(s) 79
DpnI GATC 1 cut(s) 455
DpnII GATC 1 cut(s) 453
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
FaeI CATG 2 cut(s) 103, 234
FaiI YATR 7 cut(s) 11, 101, 232, 237, 374, 403, 513
FatI CATG 2 cut(s) 99, 230
FauI CCCGC 1 cut(s) 257
Fnu4HI GCNGC 1 cut(s) 381
FokI GGATG 2 cut(s) 93, 463
Fsp4HI GCNGC 1 cut(s) 381
FspBI CTAG 2 cut(s) 221, 315
GluI GCNGC 1 cut(s) 381
HaeIII GGCC 1 cut(s) 461
HapII CCGG 1 cut(s) 445
Hin1II CATG 2 cut(s) 103, 234
HincII GTYRAC 1 cut(s) 228
HindII GTYRAC 1 cut(s) 228
HpaI GTTAAC 1 cut(s) 228
HpaII CCGG 1 cut(s) 445
HphI GGTGA 1 cut(s) 220
Hpy166II GTNNAC 1 cut(s) 228
Hpy188I TCNGA 1 cut(s) 175
Hpy188III TCNNGA 1 cut(s) 291
Hpy8I GTNNAC 1 cut(s) 228
HpyAV CCTTC 1 cut(s) 144
HpyCH4III ACNGT 2 cut(s) 208, 297
HpyCH4V TGCA 3 cut(s) 150, 242, 383
HpyF3I CTNAG 1 cut(s) 79
Hsp92II CATG 2 cut(s) 103, 234
KspAI GTTAAC 1 cut(s) 228
Kzo9I GATC 1 cut(s) 453
LmnI GCTCC 2 cut(s) 283, 310
LpnPI CCDG 3 cut(s) 17, 202, 458
Lsp1109I GCAGC 1 cut(s) 367
LweI GCATC 2 cut(s) 229, 367
MaeI CTAG 2 cut(s) 221, 315
MaeIII GTNAC 2 cut(s) 187, 387
MalI GATC 1 cut(s) 455
MboI GATC 1 cut(s) 453
MboII GAAGA 3 cut(s) 151, 206, 214
MluCI AATT 2 cut(s) 73, 486
MseI TTAA 2 cut(s) 71, 227
MspI CCGG 1 cut(s) 445
Mva1269I GAATGC 1 cut(s) 446
NdeII GATC 1 cut(s) 453
NlaIII CATG 2 cut(s) 103, 234
NlaIV GGNNCC 1 cut(s) 312
NspI RCATGY 2 cut(s) 103, 234
NspV TTCGAA 1 cut(s) 137
PciI ACATGT 2 cut(s) 99, 230
PctI GAATGC 1 cut(s) 446
PkrI GCNGC 1 cut(s) 382
PscI ACATGT 2 cut(s) 99, 230
PspN4I GGNNCC 1 cut(s) 312
PstI CTGCAG 1 cut(s) 385
SaqAI TTAA 2 cut(s) 71, 227
SatI GCNGC 1 cut(s) 381
Sau3AI GATC 1 cut(s) 453
SetI ASST 5 cut(s) 136, 288, 320, 330, 527
SfaNI GCATC 2 cut(s) 229, 367
SfcI CTRYAG 1 cut(s) 381
SfuI TTCGAA 1 cut(s) 137
Sse9I AATT 2 cut(s) 73, 486
SsiI CCGC 2 cut(s) 264, 275
SspI AATATT 1 cut(s) 120
SspMI CTAG 2 cut(s) 221, 315
TaaI ACNGT 2 cut(s) 208, 297
TaqI TCGA 4 cut(s) 137, 290, 300, 456
TasI AATT 2 cut(s) 73, 486
Tru1I TTAA 2 cut(s) 71, 227
Tru9I TTAA 2 cut(s) 71, 227
TseI GCWGC 1 cut(s) 380
TspDTI ATGAA 3 cut(s) 17, 95, 369
TspGWI ACGGA 2 cut(s) 206, 489
XapI RAATTY 1 cut(s) 73
XceI RCATGY 2 cut(s) 103, 234
XspI CTAG 2 cut(s) 221, 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.