pycom13g06330

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0016836
Physical Location & Seq
Reverse (-)
4113713 .. 4114147
435 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g06330.1

Sequence Viewer

Length: 435 bp
ATGTTTCATCCCTTTGTAATATTTTTCTCCACACCTTCGAAGAAGGTTTGCACCAATACCCGCAAATCAACTTCTGATAAAACAGAAGTAACCGAAGAAGTGAAGACGGTGATGGACAGACTAGGGTTAATATGTTGTGATGCACAAGGAGATGATATTCAAGGTGGGGTTGGTGCGGAGGAGGAGCTTTCGAGACTGTTCGATGAGGAGGAGCCTAGCTTGGAGGAGGTAAAGGAAGCGTTTGATGTGTTTGATGAAAATAAAGACGGGTTTATAGATGCTGCAGAGTTACAAAGAGTTTTATACATTTTGGGTTTGGAGGACGGGTTTGGATTGGACGAATGCCGGAGGATGATCGAGGCCGTTGATACGAACGGAGATGGACTAATTGATTTTGATGAGTTTGTGACACATATTGAGAACAGCTTTTGTTGA

Protein Analysis

145

Amino Acids

16.21

Weight (kDa)

4.15

Isoelectric Point (pI)

52.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 75 - 138 1.6e-14 EF-hand domain pair
EF-hand_1 PF00036 76 - 101 2.6e-07 EF hand domain
EF-hand_6 PF13405 76 - 105 1.9e-07 EF-hand domain
EF-hand_5 PF13202 78 - 101 2.6e-07 EF hand
EF-hand_8 PF13833 89 - 136 2.1e-08 EF-hand domain pair
EF-hand_1 PF00036 114 - 137 3.5e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016836)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31200
malus_domestica MD13G1070500.v1.1
prunus_persica Prupe.1G276100_v2.0.a1
pyrus_communis pycom13g06330
rosa_chinensis RchiOBHm_Chr4g0439581
rosa_laevigata RLG00000006256
rosa_multiflora Rmu_sc0002549.1_g000024
rosa_roxburghii Rroxscaffold_5G00380520
rosa_rugosa Rorug04G0320000
rosa_samantha Rh4AG371000 Rh4BG382800 Rh4CG397400 Rh4DG377600
rosa_wichuraiana Rw4G031630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 61, 176
AgsI TTSAA 1 cut(s) 161
AluBI AGCT 3 cut(s) 187, 219, 426
AluI AGCT 3 cut(s) 187, 219, 426
Alw26I GTCTC 1 cut(s) 187
AoxI GGCC 1 cut(s) 360
ApeKI GCWGC 1 cut(s) 281
AsuHPI GGTGA 1 cut(s) 121
AsuII TTCGAA 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 110
BbvI GCAGC 1 cut(s) 268
BccI CCATC 2 cut(s) 106, 374
BceAI ACGGC 1 cut(s) 347
BcoDI GTCTC 1 cut(s) 187
BfaI CTAG 2 cut(s) 122, 216
BfmI CTRYAG 1 cut(s) 282
BisI GCNGC 1 cut(s) 282
BlsI GCNGC 1 cut(s) 283
BmiI GGNNCC 1 cut(s) 213
BmsI GCATC 2 cut(s) 130, 268
BpiI GAAGAC 1 cut(s) 110
Bpu14I TTCGAA 1 cut(s) 38
BsaXI ACNNNNNCTCC 2 cut(s) 311, 341
BseGI GGATG 2 cut(s) 7, 357
BseRI GAGGAG 5 cut(s) 194, 197, 221, 224, 239
BseXI GCAGC 1 cut(s) 268
BshFI GGCC 1 cut(s) 362
BsiSI CCGG 1 cut(s) 346
BsmAI GTCTC 1 cut(s) 187
BsmI GAATGC 1 cut(s) 347
BsnI GGCC 1 cut(s) 362
Bsp119I TTCGAA 1 cut(s) 38
Bsp143I GATC 1 cut(s) 354
BspACI CCGC 2 cut(s) 61, 176
BspANI GGCC 1 cut(s) 362
BspLI GGNNCC 1 cut(s) 213
BspMAI CTGCAG 1 cut(s) 286
BspT104I TTCGAA 1 cut(s) 38
BssMI GATC 1 cut(s) 354
Bst4CI ACNGT 2 cut(s) 109, 198
BstBI TTCGAA 1 cut(s) 38
BstF5I GGATG 2 cut(s) 7, 357
BstKTI GATC 1 cut(s) 357
BstMAI GTCTC 1 cut(s) 187
BstMBI GATC 1 cut(s) 354
BstSFI CTRYAG 1 cut(s) 282
BstV1I GCAGC 1 cut(s) 268
BstV2I GAAGAC 1 cut(s) 110
BsuRI GGCC 1 cut(s) 362
BtsCI GGATG 2 cut(s) 7, 357
CviJI RGCY 5 cut(s) 187, 214, 219, 362, 426
CviKI_1 RGCY 5 cut(s) 187, 214, 219, 362, 426
DpnI GATC 1 cut(s) 356
DpnII GATC 1 cut(s) 354
FaiI YATR 4 cut(s) 133, 275, 304, 414
FauI CCCGC 1 cut(s) 68
Fnu4HI GCNGC 1 cut(s) 282
FokI GGATG 1 cut(s) 364
Fsp4HI GCNGC 1 cut(s) 282
FspBI CTAG 2 cut(s) 122, 216
GluI GCNGC 1 cut(s) 282
HaeIII GGCC 1 cut(s) 362
HapII CCGG 1 cut(s) 346
HpaII CCGG 1 cut(s) 346
HphI GGTGA 1 cut(s) 121
Hpy188I TCNGA 1 cut(s) 76
Hpy188III TCNNGA 1 cut(s) 192
HpyAV CCTTC 2 cut(s) 37, 45
HpyCH4III ACNGT 2 cut(s) 109, 198
HpyCH4V TGCA 3 cut(s) 51, 143, 284
Kzo9I GATC 1 cut(s) 354
LmnI GCTCC 2 cut(s) 184, 211
LpnPI CCDG 1 cut(s) 359
Lsp1109I GCAGC 1 cut(s) 268
LweI GCATC 2 cut(s) 130, 268
MaeI CTAG 2 cut(s) 122, 216
MaeIII GTNAC 3 cut(s) 88, 288, 406
MalI GATC 1 cut(s) 356
MboI GATC 1 cut(s) 354
MboII GAAGA 3 cut(s) 52, 107, 115
MluCI AATT 1 cut(s) 387
MnlI CCTC 9 cut(s) 172, 175, 199, 202, 217, 220, 313, 342, 352
MseI TTAA 1 cut(s) 128
MspI CCGG 1 cut(s) 346
Mva1269I GAATGC 1 cut(s) 347
NdeII GATC 1 cut(s) 354
NlaIV GGNNCC 1 cut(s) 213
NmuCI GTSAC 1 cut(s) 406
NspV TTCGAA 1 cut(s) 38
PctI GAATGC 1 cut(s) 347
PkrI GCNGC 1 cut(s) 283
PspN4I GGNNCC 1 cut(s) 213
PstI CTGCAG 1 cut(s) 286
SaqAI TTAA 1 cut(s) 128
SatI GCNGC 1 cut(s) 282
Sau3AI GATC 1 cut(s) 354
SetI ASST 7 cut(s) 37, 48, 166, 189, 221, 231, 428
SfaNI GCATC 2 cut(s) 130, 268
SfcI CTRYAG 1 cut(s) 282
SfuI TTCGAA 1 cut(s) 38
Sse9I AATT 1 cut(s) 387
SsiI CCGC 2 cut(s) 61, 176
SspI AATATT 1 cut(s) 21
SspMI CTAG 2 cut(s) 122, 216
TaaI ACNGT 2 cut(s) 109, 198
TaqI TCGA 4 cut(s) 38, 191, 201, 357
TasI AATT 1 cut(s) 387
Tru1I TTAA 1 cut(s) 128
Tru9I TTAA 1 cut(s) 128
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 1 cut(s) 281
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 1 cut(s) 270
TspGWI ACGGA 1 cut(s) 390
XspI CTAG 2 cut(s) 122, 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.