FvH4_4g32790

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014728
Physical Location & Seq
Forward (+)
31293599 .. 31296128
2530 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g32790.t1

Sequence Viewer

Length: 840 bp
ATGGCAATGGTACTCTCGCAGCAGCTTGATCATCATCATCAGAGCAGCGCCGCCCGTATACATAGGATGCCGGCTGTGCCTTTGTCCCTGGCAGCCATCCAAGAATTCGGAGAAGACTGCTACCTCTACTCCGACTCTGATGATGAGGGGAGTTTGTTCTCTATTGATTTCAACTGTATCAATCACAAGAACATGAAGCCGGATCAAATCGTGCAGGAAGAGAAGGTTTATCACGTAGCAGTTGACCTGATGAAGAGTGAGACTAGCATGGATGCCCTTCTCTGGACGCTCAGCCAGGCCGTTGCTCATCCATCATCCAATATCAAAACCTTGTATCACGTCAAACTCGTACATGTCTTTCCACCAGTTCATTTCATTCCTTCTCCATTAGGAATGCTTCCAAGAAGTAAAGTGAGTCCAAAGATGGTGAAGAATTACCTGGCTCAAGAAAGAGACAGAAGAACCAAGCTCCTTGAGAAATACGTTGCTGCATGCTCAACGGCCAAGATTAAGGTCGATGTTATGTTTATTGAGAGTGGTACAACTGCAAAGGCCATCCTAGACCTTATTTCGACTGGAAACGTGAAGATTCTAGTTGTTGGAACAACGAAATCCAGTCTCAGGAAACTGAGGTCTAGAAAGGGAACTGGGGTTGCTAATCAGATACTACGAAATGCATCTCCAGAAACGAGCTGCTGCGATATAAAGATCATATGCAAGGGAAAGGAACTGGTGATTGATCAGATTATCGGATCGCCATCCTCGCGTAGCAGTAATGCAAATTCCTTATCAACGCAAGAGGATCAAGATCAAGATCGAAGTAGCAATGTGATGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

280

Amino Acids

30.91

Weight (kDa)

8.66

Isoelectric Point (pI)

54.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014728)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g32790 FvH4_4g32790
malus_domestica MD13G1022500.v1.1 MD16G1023800.v1.1
prunus_persica Prupe.1G329100_v2.0.a1
pyrus_communis pycom13g02100
rosa_chinensis RchiOBHm_Chr4g0441781
rosa_laevigata RLG00000006066
rosa_multiflora Rmu_sc0013960.1_g000007 Rmu_ssc0000486.1_g000031
rosa_roxburghii Rroxscaffold_5G00382610
rosa_rugosa Rorug04G0336200
rosa_samantha Rh4AG387500 Rh4BG401300 Rh4CG416300 Rh4DG394500
rosa_wichuraiana Rw4G033450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 58
AccII CGCG 1 cut(s) 766
AciI CCGC 1 cut(s) 51
AclWI GGATC 3 cut(s) 210, 760, 810
AcoI YGGCCR 1 cut(s) 501
AcsI RAATTY 2 cut(s) 104, 781
AfaI GTAC 3 cut(s) 12, 351, 541
AfiI CCNNNNNNNGG 2 cut(s) 282, 621
AflIII ACRYGT 1 cut(s) 352
AgsI TTSAA 1 cut(s) 172
AjiI CACGTC 1 cut(s) 340
AjnI CCWGG 3 cut(s) 87, 294, 438
AluBI AGCT 3 cut(s) 25, 469, 693
AluI AGCT 3 cut(s) 25, 469, 693
Alw26I GTCTC 3 cut(s) 254, 447, 623
AlwI GGATC 3 cut(s) 210, 760, 810
AoxI GGCC 3 cut(s) 297, 501, 552
ApeKI GCWGC 7 cut(s) 19, 22, 45, 92, 488, 693, 696
ApoI RAATTY 2 cut(s) 104, 781
AspLEI GCGC 1 cut(s) 50
AsuHPI GGTGA 2 cut(s) 439, 745
BbsI GAAGAC 1 cut(s) 120
BbvI GCAGC 7 cut(s) 31, 34, 57, 104, 475, 680, 683
BccI CCATC 5 cut(s) 104, 319, 418, 563, 766
BceAI ACGGC 2 cut(s) 284, 516
BciT130I CCWGG 3 cut(s) 89, 296, 440
BclI TGATCA 2 cut(s) 28, 739
BcoDI GTCTC 3 cut(s) 254, 447, 623
BfaI CTAG 4 cut(s) 264, 560, 593, 636
BfoI RGCGCY 1 cut(s) 51
BisI GCNGC 8 cut(s) 20, 23, 46, 51, 93, 489, 694, 697
BlpI GCTNAGC 1 cut(s) 290
BlsI GCNGC 8 cut(s) 21, 24, 47, 52, 94, 490, 695, 698
Bme1390I CCNGG 3 cut(s) 89, 296, 440
BmgBI CACGTC 1 cut(s) 340
BmrFI CCNGG 3 cut(s) 89, 296, 440
BmrI ACTGGG 1 cut(s) 657
BmsI GCATC 3 cut(s) 57, 262, 686
BmuI ACTGGG 1 cut(s) 657
BpiI GAAGAC 1 cut(s) 120
BpmI CTGGAG 1 cut(s) 666
Bpu1102I GCTNAGC 1 cut(s) 290
BpuEI CTTGAG 2 cut(s) 429, 494
BsaAI YACGTR 1 cut(s) 235
BsaBI GATNNNNATC 4 cut(s) 33, 757, 807, 813
BsaJI CCNNGG 1 cut(s) 87
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 2 cut(s) 282, 621
Bse118I RCCGGY 1 cut(s) 70
Bse1I ACTGG 5 cut(s) 365, 580, 615, 652, 735
Bse3DI GCAATG 2 cut(s) 12, 832
Bse8I GATNNNNATC 4 cut(s) 33, 757, 807, 813
BseBI CCWGG 3 cut(s) 89, 296, 440
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 7 cut(s) 72, 96, 277, 307, 314, 555, 758
BseJI GATNNNNATC 4 cut(s) 33, 757, 807, 813
BseLI CCNNNNNNNGG 2 cut(s) 282, 621
BseMI GCAATG 2 cut(s) 12, 832
BseMII CTCAG 3 cut(s) 304, 620, 634
BseNI ACTGG 5 cut(s) 365, 580, 615, 652, 735
BseXI GCAGC 7 cut(s) 31, 34, 57, 104, 475, 680, 683
BsgI GTGCAG 1 cut(s) 233
Bsh1236I CGCG 1 cut(s) 766
BshFI GGCC 3 cut(s) 299, 503, 554
BsiSI CCGG 2 cut(s) 71, 200
BslFI GGGAC 1 cut(s) 70
BslI CCNNNNNNNGG 2 cut(s) 282, 621
BsmAI GTCTC 3 cut(s) 254, 447, 623
BsmFI GGGAC 1 cut(s) 70
BsmI GAATGC 1 cut(s) 399
BsnI GGCC 3 cut(s) 299, 503, 554
Bsp143I GATC 8 cut(s) 28, 202, 708, 739, 752, 802, 808, 814
Bsp1720I GCTNAGC 1 cut(s) 290
BspACI CCGC 1 cut(s) 51
BspANI GGCC 3 cut(s) 299, 503, 554
BspCNI CTCAG 3 cut(s) 303, 621, 633
BspFNI CGCG 1 cut(s) 766
BspPI GGATC 3 cut(s) 210, 760, 810
BsrDI GCAATG 2 cut(s) 12, 832
BsrFI RCCGGY 1 cut(s) 70
BsrI ACTGG 5 cut(s) 365, 580, 615, 652, 735
BssAI RCCGGY 1 cut(s) 70
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 8 cut(s) 28, 202, 708, 739, 752, 802, 808, 814
BssNAI GTATAC 1 cut(s) 59
Bst1107I GTATAC 1 cut(s) 59
Bst2UI CCWGG 3 cut(s) 89, 296, 440
Bst4CI ACNGT 1 cut(s) 176
Bst6I CTCTTC 2 cut(s) 213, 248
BstBAI YACGTR 1 cut(s) 235
BstC8I GCNNGC 2 cut(s) 72, 493
BstDEI CTNAG 3 cut(s) 290, 620, 629
BstF5I GGATG 7 cut(s) 72, 96, 277, 307, 314, 555, 758
BstFNI CGCG 1 cut(s) 766
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 1 cut(s) 50
BstKTI GATC 8 cut(s) 31, 205, 711, 742, 755, 805, 811, 817
BstMAI GTCTC 3 cut(s) 254, 447, 623
BstMBI GATC 8 cut(s) 28, 202, 708, 739, 752, 802, 808, 814
BstMWI GCNNNNNNNGC 2 cut(s) 76, 763
BstNI CCWGG 3 cut(s) 89, 296, 440
BstNSI RCATGY 2 cut(s) 356, 495
BstSCI CCNGG 3 cut(s) 87, 294, 438
BstUI CGCG 1 cut(s) 766
BstV1I GCAGC 7 cut(s) 31, 34, 57, 104, 475, 680, 683
BstV2I GAAGAC 1 cut(s) 120
BstZ17I GTATAC 1 cut(s) 59
BsuRI GGCC 3 cut(s) 299, 503, 554
BtrI CACGTC 1 cut(s) 340
BtsCI GGATG 7 cut(s) 72, 96, 277, 307, 314, 555, 758
Cac8I GCNNGC 2 cut(s) 72, 493
CfoI GCGC 1 cut(s) 50
Cfr10I RCCGGY 1 cut(s) 70
CseI GACGC 1 cut(s) 295
Csp6I GTAC 3 cut(s) 11, 350, 540
CviAII CATG 4 cut(s) 193, 268, 353, 492
CviQI GTAC 3 cut(s) 11, 350, 540
DdeI CTNAG 3 cut(s) 290, 620, 629
DpnI GATC 8 cut(s) 30, 204, 710, 741, 754, 804, 810, 816
DpnII GATC 8 cut(s) 28, 202, 708, 739, 752, 802, 808, 814
EaeI YGGCCR 1 cut(s) 501
Eam1104I CTCTTC 2 cut(s) 213, 248
EarI CTCTTC 2 cut(s) 213, 248
EcoRI GAATTC 1 cut(s) 104
EcoRII CCWGG 3 cut(s) 87, 294, 438
EcoT22I ATGCAT 1 cut(s) 679
FaeI CATG 4 cut(s) 196, 271, 356, 495
FaqI GGGAC 1 cut(s) 70
FatI CATG 4 cut(s) 192, 267, 352, 491
FauNDI CATATG 1 cut(s) 713
FbaI TGATCA 2 cut(s) 28, 739
FblI GTMKAC 1 cut(s) 58
Fnu4HI GCNGC 8 cut(s) 20, 23, 46, 51, 93, 489, 694, 697
FokI GGATG 7 cut(s) 79, 83, 284, 294, 301, 542, 745
Fsp4HI GCNGC 8 cut(s) 20, 23, 46, 51, 93, 489, 694, 697
FspBI CTAG 4 cut(s) 264, 560, 593, 636
GlaI GCGC 1 cut(s) 49
GluI GCNGC 8 cut(s) 20, 23, 46, 51, 93, 489, 694, 697
GsuI CTGGAG 1 cut(s) 666
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 3 cut(s) 299, 503, 554
HapII CCGG 2 cut(s) 71, 200
HgaI GACGC 1 cut(s) 295
HhaI GCGC 1 cut(s) 50
Hin1II CATG 4 cut(s) 196, 271, 356, 495
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HinfI GANTC 3 cut(s) 134, 415, 589
HpaII CCGG 2 cut(s) 71, 200
HphI GGTGA 2 cut(s) 439, 745
Hpy166II GTNNAC 2 cut(s) 59, 244
Hpy188I TCNGA 7 cut(s) 42, 110, 133, 139, 663, 744, 752
Hpy188III TCNNGA 7 cut(s) 283, 446, 622, 636, 683, 806, 812
Hpy8I GTNNAC 2 cut(s) 59, 244
HpyAV CCTTC 3 cut(s) 217, 287, 390
HpyCH4III ACNGT 1 cut(s) 176
HpyCH4IV ACGT 4 cut(s) 234, 339, 483, 582
HpyCH4V TGCA 6 cut(s) 214, 491, 548, 677, 717, 779
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 763
HpyF3I CTNAG 3 cut(s) 290, 620, 629
HpySE526I ACGT 4 cut(s) 234, 339, 483, 582
Hsp92II CATG 4 cut(s) 196, 271, 356, 495
HspAI GCGC 1 cut(s) 48
KroI GCCGGC 1 cut(s) 70
KroNI GCCGGC 1 cut(s) 72
Ksp22I TGATCA 2 cut(s) 28, 739
Kzo9I GATC 8 cut(s) 28, 202, 708, 739, 752, 802, 808, 814
LmnI GCTCC 1 cut(s) 474
Lsp1109I GCAGC 7 cut(s) 31, 34, 57, 104, 475, 680, 683
LweI GCATC 3 cut(s) 57, 262, 686
MaeI CTAG 4 cut(s) 264, 560, 593, 636
MaeII ACGT 4 cut(s) 234, 339, 483, 582
MalI GATC 8 cut(s) 30, 204, 710, 741, 754, 804, 810, 816
MboI GATC 8 cut(s) 28, 202, 708, 739, 752, 802, 808, 814
MboII GAAGA 6 cut(s) 125, 230, 265, 442, 471, 598
MluCI AATT 3 cut(s) 104, 433, 781
MlyI GAGTC 2 cut(s) 128, 424
MmeI TCCRAC 2 cut(s) 156, 580
MnlI CCTC 5 cut(s) 134, 139, 624, 772, 793
Mph1103I ATGCAT 1 cut(s) 679
MroNI GCCGGC 1 cut(s) 70
MseI TTAA 1 cut(s) 510
MspI CCGG 2 cut(s) 71, 200
MspR9I CCNGG 3 cut(s) 89, 296, 440
Mva1269I GAATGC 1 cut(s) 399
MvaI CCWGG 3 cut(s) 89, 296, 440
MvnI CGCG 1 cut(s) 766
MwoI GCNNNNNNNGC 2 cut(s) 76, 763
NaeI GCCGGC 1 cut(s) 72
NdeI CATATG 1 cut(s) 713
NdeII GATC 8 cut(s) 28, 202, 708, 739, 752, 802, 808, 814
NgoMIV GCCGGC 1 cut(s) 70
NlaIII CATG 4 cut(s) 196, 271, 356, 495
NsiI ATGCAT 1 cut(s) 679
NspI RCATGY 2 cut(s) 356, 495
PaeI GCATGC 1 cut(s) 495
PciI ACATGT 1 cut(s) 352
PcsI WCGNNNNNNNCGW 1 cut(s) 345
PctI GAATGC 1 cut(s) 399
PdiI GCCGGC 1 cut(s) 72
PfeI GAWTC 1 cut(s) 589
PkrI GCNGC 8 cut(s) 21, 24, 47, 52, 94, 490, 695, 698
PleI GAGTC 2 cut(s) 128, 423
PpsI GAGTC 2 cut(s) 128, 423
Ppu21I YACGTR 1 cut(s) 235
PscI ACATGT 1 cut(s) 352
Psp6I CCWGG 3 cut(s) 87, 294, 438
PspGI CCWGG 3 cut(s) 87, 294, 438
RsaI GTAC 3 cut(s) 12, 351, 541
RsaNI GTAC 3 cut(s) 11, 350, 540
SaqAI TTAA 1 cut(s) 510
SatI GCNGC 8 cut(s) 20, 23, 46, 51, 93, 489, 694, 697
Sau3AI GATC 8 cut(s) 28, 202, 708, 739, 752, 802, 808, 814
SchI GAGTC 2 cut(s) 128, 424
ScrFI CCNGG 3 cut(s) 89, 296, 440
SfaNI GCATC 3 cut(s) 57, 262, 686
SmlI CTYRAG 2 cut(s) 444, 473
SmoI CTYRAG 2 cut(s) 444, 473
SphI GCATGC 1 cut(s) 495
Sse9I AATT 3 cut(s) 104, 433, 781
SsiI CCGC 1 cut(s) 51
SspMI CTAG 4 cut(s) 264, 560, 593, 636
StyD4I CCNGG 3 cut(s) 87, 294, 438
TaaI ACNGT 1 cut(s) 176
TaiI ACGT 4 cut(s) 237, 342, 486, 585
TaqI TCGA 3 cut(s) 516, 572, 817
TasI AATT 3 cut(s) 104, 433, 781
TauI GCSGC 1 cut(s) 53
TfiI GAWTC 1 cut(s) 589
Tru1I TTAA 1 cut(s) 510
Tru9I TTAA 1 cut(s) 510
TseI GCWGC 7 cut(s) 19, 22, 45, 92, 488, 693, 696
TspDTI ATGAA 4 cut(s) 209, 266, 359, 364
XapI RAATTY 2 cut(s) 104, 781
XbaI TCTAGA 1 cut(s) 635
XceI RCATGY 2 cut(s) 356, 495
XmiI GTMKAC 1 cut(s) 58
XspI CTAG 4 cut(s) 264, 560, 593, 636
Zsp2I ATGCAT 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.