MD13G1022500.v1.1

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014728
Physical Location & Seq
Reverse (-)
1619144 .. 1622114
2971 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1022500.v1.1.491

Sequence Viewer

Length: 900 bp
ATGACAAGGGAAGAGACGAGGATGCCCTCGCCCTTGCTCTTGCCCTTGACGTCAATTGAAGAAGAATTAGAAGCTGCTTTTGATGACGATGATGATGATGAAAGTCTGTTTTCCATTGATTTTAATTTTAACTTCAAGGATAGTTTTAAGGATAGTTTTAAGGAAAAGGAAGAGGCCGTTCCTAATTATTATCACGTAGCTGTTGGGGGGCAGAAGAGCGAGTCTAGCATGGATGCGCTTCTCTGGGCACTGAGTCACACGGCGGTGACTCATGATCATGATCAATCTTCAGTAATACTAGTCCTCGTCCATGTCTTTGCACCGATCCGATACGTTCCTTCTCCTTTAGGAATGCTTCCAAAAAATAGTGTGAATACAATTATGATGGAGAATTGGATGGCCCTAGAAAGAGACAGAAGGAGGAAACTCCTTGACAAATACGTTGATGCCTGCTCATCTGCCAAGGTTAAGACGGAGATTATGCTTATTGAGAGTGATACAGTTGCCAAGGCAATCCTAGAACTTATTCCTACTCAAAACATAAGAAACCTAGTAGTTGGAACCACCAAATCTAGTCTGAGGAAACTGAGGTCCAAGAAAGGGAGTGGAATAGGAAGTCAGATCCTACAAAATGCGCCCGAAACAAGCTGCAATATTAAGATCATATGCAAGGGAAAGGAAGTGATTGATGACACTCTGTTTACTGGCTCGACTTCCTCGCGTAGCAGTAACGCGAACTCCTTGTCCACGCAAGATGAAGACGACCATCAAGAACAACGAATCTCAACAGATTATAATAAACATGAGTACTCGCGTCCTGAAGCGAAATCTCCATCATCTTTAAATGGATACAAGGAAATGTGGTGGGGGAAATCAAAATCTTCACAAATAACAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

300

Amino Acids

33.61

Weight (kDa)

5.38

Isoelectric Point (pI)

56.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014728)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g32790 FvH4_4g32790
malus_domestica MD13G1022500.v1.1 MD16G1023800.v1.1
prunus_persica Prupe.1G329100_v2.0.a1
pyrus_communis pycom13g02100
rosa_chinensis RchiOBHm_Chr4g0441781
rosa_laevigata RLG00000006066
rosa_multiflora Rmu_sc0013960.1_g000007 Rmu_ssc0000486.1_g000031
rosa_roxburghii Rroxscaffold_5G00382610
rosa_rugosa Rorug04G0336200
rosa_samantha Rh4AG387500 Rh4BG401300 Rh4CG416300 Rh4DG394500
rosa_wichuraiana Rw4G033450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 795
AatII GACGTC 1 cut(s) 53
AccII CGCG 3 cut(s) 721, 734, 814
AciI CCGC 1 cut(s) 263
AclWI GGATC 2 cut(s) 319, 616
AcuI CTGAAG 2 cut(s) 273, 840
AcyI GRCGYC 1 cut(s) 50
AfaI GTAC 1 cut(s) 809
AfiI CCNNNNNNNGG 1 cut(s) 600
AgsI TTSAA 2 cut(s) 59, 136
AhlI ACTAGT 1 cut(s) 298
AleI CACNNNNGTG 1 cut(s) 263
AloI GAACNNNNNNTCC 2 cut(s) 728, 760
AluBI AGCT 4 cut(s) 74, 200, 648, 896
AluI AGCT 4 cut(s) 74, 200, 648, 896
Alw26I GTCTC 2 cut(s) 8, 405
AlwI GGATC 2 cut(s) 319, 616
AoxI GGCC 2 cut(s) 174, 399
ApeKI GCWGC 2 cut(s) 74, 648
ArsI GACNNNNNNTTYG 2 cut(s) 728, 760
Asp700I GAANNNNTTC 1 cut(s) 525
AspLEI GCGC 2 cut(s) 238, 637
AspS9I GGNCC 2 cut(s) 400, 591
AsuHPI GGTGA 1 cut(s) 277
AvaII GGWCC 1 cut(s) 591
BaeGI GKGCMC 1 cut(s) 250
BbsI GAAGAC 1 cut(s) 765
BbvI GCAGC 2 cut(s) 61, 635
BccI CCATC 4 cut(s) 379, 391, 774, 841
BceAI ACGGC 2 cut(s) 161, 276
BciVI GTATCC 1 cut(s) 842
BclI TGATCA 2 cut(s) 274, 280
BcoDI GTCTC 2 cut(s) 8, 405
BcuI ACTAGT 1 cut(s) 298
BfaI CTAG 6 cut(s) 225, 299, 404, 518, 551, 573
BfuI GTATCC 1 cut(s) 842
BisI GCNGC 2 cut(s) 75, 649
BlsI GCNGC 2 cut(s) 76, 650
BmcAI AGTACT 1 cut(s) 809
Bme18I GGWCC 1 cut(s) 591
BmgT120I GGNCC 2 cut(s) 400, 591
BmiI GGNNCC 1 cut(s) 562
BmsI GCATC 3 cut(s) 12, 223, 436
BpiI GAAGAC 1 cut(s) 765
BsaAI YACGTR 1 cut(s) 196
BsaBI GATNNNNATC 1 cut(s) 279
BsaHI GRCGYC 1 cut(s) 50
BsaJI CCNNGG 2 cut(s) 462, 507
BsaXI ACNNNNNCTCC 2 cut(s) 722, 752
Bsc4I CCNNNNNNNGG 1 cut(s) 600
Bse1I ACTGG 1 cut(s) 709
Bse8I GATNNNNATC 1 cut(s) 279
BseDI CCNNGG 2 cut(s) 462, 507
BseGI GGATG 3 cut(s) 27, 238, 402
BseJI GATNNNNATC 1 cut(s) 279
BseLI CCNNNNNNNGG 1 cut(s) 600
BseMII CTCAG 3 cut(s) 242, 569, 578
BseNI ACTGG 1 cut(s) 709
BseSI GKGCMC 1 cut(s) 250
BseXI GCAGC 2 cut(s) 61, 635
Bsh1236I CGCG 3 cut(s) 721, 734, 814
BshFI GGCC 2 cut(s) 176, 401
BslI CCNNNNNNNGG 1 cut(s) 600
BsmAI GTCTC 2 cut(s) 8, 405
BsmBI CGTCTC 1 cut(s) 8
BsmI GAATGC 1 cut(s) 357
BsnI GGCC 2 cut(s) 176, 401
Bsp1286I GDGCHC 1 cut(s) 250
Bsp143I GATC 5 cut(s) 274, 280, 324, 621, 660
BspACI CCGC 1 cut(s) 263
BspANI GGCC 2 cut(s) 176, 401
BspCNI CTCAG 3 cut(s) 243, 570, 579
BspFNI CGCG 3 cut(s) 721, 734, 814
BspHI TCATGA 2 cut(s) 271, 277
BspLI GGNNCC 1 cut(s) 562
BspPI GGATC 2 cut(s) 319, 616
BspQI GCTCTTC 1 cut(s) 209
BsrI ACTGG 1 cut(s) 709
BssECI CCNNGG 2 cut(s) 462, 507
BssMI GATC 5 cut(s) 274, 280, 324, 621, 660
BssNI GRCGYC 1 cut(s) 50
BssT1I CCWWGG 2 cut(s) 462, 507
Bst4CI ACNGT 1 cut(s) 502
Bst6I CTCTTC 3 cut(s) 6, 165, 209
BstACI GRCGYC 1 cut(s) 50
BstBAI YACGTR 1 cut(s) 196
BstC8I GCNNGC 1 cut(s) 451
BstDEI CTNAG 3 cut(s) 251, 578, 587
BstF5I GGATG 3 cut(s) 27, 238, 402
BstFNI CGCG 3 cut(s) 721, 734, 814
BstHHI GCGC 2 cut(s) 238, 637
BstKTI GATC 5 cut(s) 277, 283, 327, 624, 663
BstMAI GTCTC 2 cut(s) 8, 405
BstMBI GATC 5 cut(s) 274, 280, 324, 621, 660
BstMWI GCNNNNNNNGC 1 cut(s) 225
BstSLI GKGCMC 1 cut(s) 250
BstUI CGCG 3 cut(s) 721, 734, 814
BstV1I GCAGC 2 cut(s) 61, 635
BstV2I GAAGAC 1 cut(s) 765
BstX2I RGATCY 1 cut(s) 621
BstYI RGATCY 1 cut(s) 621
BsuI GTATCC 1 cut(s) 842
BsuRI GGCC 2 cut(s) 176, 401
BtsCI GGATG 3 cut(s) 27, 238, 402
BtsIMutI CAGTG 1 cut(s) 248
Cac8I GCNNGC 1 cut(s) 451
CciI TCATGA 2 cut(s) 271, 277
CfoI GCGC 2 cut(s) 238, 637
Cfr13I GGNCC 2 cut(s) 400, 591
CseI GACGC 1 cut(s) 803
Csp6I GTAC 1 cut(s) 808
CviAII CATG 5 cut(s) 229, 272, 278, 311, 803
CviJI RGCY 7 cut(s) 74, 176, 200, 401, 648, 708, 896
CviKI_1 RGCY 7 cut(s) 74, 176, 200, 401, 648, 708, 896
CviQI GTAC 1 cut(s) 808
DdeI CTNAG 3 cut(s) 251, 578, 587
DpnI GATC 5 cut(s) 276, 282, 326, 623, 662
DpnII GATC 5 cut(s) 274, 280, 324, 621, 660
DraI TTTAAA 1 cut(s) 843
Eam1104I CTCTTC 3 cut(s) 6, 165, 209
EarI CTCTTC 3 cut(s) 6, 165, 209
Eco130I CCWWGG 2 cut(s) 462, 507
Eco47I GGWCC 1 cut(s) 591
Eco57I CTGAAG 2 cut(s) 273, 840
EcoT14I CCWWGG 2 cut(s) 462, 507
ErhI CCWWGG 2 cut(s) 462, 507
Esp3I CGTCTC 1 cut(s) 8
FaeI CATG 5 cut(s) 232, 275, 281, 314, 806
FatI CATG 5 cut(s) 228, 271, 277, 310, 802
FauNDI CATATG 1 cut(s) 665
FbaI TGATCA 2 cut(s) 274, 280
Fnu4HI GCNGC 2 cut(s) 75, 649
FokI GGATG 3 cut(s) 34, 245, 409
Fsp4HI GCNGC 2 cut(s) 75, 649
FspBI CTAG 6 cut(s) 225, 299, 404, 518, 551, 573
GlaI GCGC 2 cut(s) 237, 636
GluI GCNGC 2 cut(s) 75, 649
HaeIII GGCC 2 cut(s) 176, 401
HgaI GACGC 1 cut(s) 803
HhaI GCGC 2 cut(s) 238, 637
Hin1I GRCGYC 1 cut(s) 50
Hin1II CATG 5 cut(s) 232, 275, 281, 314, 806
Hin6I GCGC 2 cut(s) 236, 635
HinP1I GCGC 2 cut(s) 236, 635
HinfI GANTC 4 cut(s) 221, 253, 268, 780
HphI GGTGA 1 cut(s) 277
Hpy166II GTNNAC 2 cut(s) 702, 747
Hpy188I TCNGA 3 cut(s) 329, 579, 621
Hpy188III TCNNGA 4 cut(s) 272, 278, 770, 818
Hpy8I GTNNAC 2 cut(s) 702, 747
HpyAV CCTTC 2 cut(s) 348, 411
HpyCH4III ACNGT 1 cut(s) 502
HpyCH4IV ACGT 4 cut(s) 50, 195, 333, 441
HpyCH4V TGCA 3 cut(s) 320, 651, 669
HpyF10VI GCNNNNNNNGC 1 cut(s) 225
HpyF3I CTNAG 3 cut(s) 251, 578, 587
HpySE526I ACGT 4 cut(s) 50, 195, 333, 441
Hsp92I GRCGYC 1 cut(s) 50
Hsp92II CATG 5 cut(s) 232, 275, 281, 314, 806
HspAI GCGC 2 cut(s) 236, 635
Ksp22I TGATCA 2 cut(s) 274, 280
Kzo9I GATC 5 cut(s) 274, 280, 324, 621, 660
LguI GCTCTTC 1 cut(s) 209
LpnPI CCDG 4 cut(s) 229, 463, 690, 831
Lsp1109I GCAGC 2 cut(s) 61, 635
LweI GCATC 3 cut(s) 12, 223, 436
MaeI CTAG 6 cut(s) 225, 299, 404, 518, 551, 573
MaeII ACGT 4 cut(s) 50, 195, 333, 441
MaeIII GTNAC 3 cut(s) 254, 265, 728
MalI GATC 5 cut(s) 276, 282, 326, 623, 662
MboI GATC 5 cut(s) 274, 280, 324, 621, 660
MboII GAAGA 8 cut(s) 23, 71, 74, 182, 226, 279, 770, 873
MfeI CAATTG 1 cut(s) 54
MflI RGATCY 1 cut(s) 621
MhlI GDGCHC 1 cut(s) 250
MluCI AATT 6 cut(s) 54, 65, 124, 184, 378, 391
MlyI GAGTC 3 cut(s) 230, 262, 262
MmeI TCCRAC 1 cut(s) 538
MnlI CCTC 8 cut(s) 12, 37, 166, 314, 414, 573, 582, 727
MroXI GAANNNNTTC 1 cut(s) 525
MseI TTAA 7 cut(s) 123, 129, 147, 159, 468, 657, 842
MslI CAYNNNNRTG 2 cut(s) 263, 276
MunI CAATTG 1 cut(s) 54
Mva1269I GAATGC 1 cut(s) 357
MvnI CGCG 3 cut(s) 721, 734, 814
MwoI GCNNNNNNNGC 1 cut(s) 225
NdeI CATATG 1 cut(s) 665
NdeII GATC 5 cut(s) 274, 280, 324, 621, 660
NlaIII CATG 5 cut(s) 232, 275, 281, 314, 806
NlaIV GGNNCC 1 cut(s) 562
NmuCI GTSAC 2 cut(s) 254, 265
OliI CACNNNNGTG 1 cut(s) 263
PagI TCATGA 2 cut(s) 271, 277
PciSI GCTCTTC 1 cut(s) 209
PctI GAATGC 1 cut(s) 357
PdmI GAANNNNTTC 1 cut(s) 525
PfeI GAWTC 1 cut(s) 780
PkrI GCNGC 2 cut(s) 76, 650
PleI GAGTC 3 cut(s) 229, 261, 262
PpsI GAGTC 3 cut(s) 229, 261, 262
Ppu21I YACGTR 1 cut(s) 196
PsiI TTATAA 1 cut(s) 795
PspN4I GGNNCC 1 cut(s) 562
PspPI GGNCC 2 cut(s) 400, 591
PsuI RGATCY 1 cut(s) 621
RsaI GTAC 1 cut(s) 809
RsaNI GTAC 1 cut(s) 808
RseI CAYNNNNRTG 2 cut(s) 263, 276
SapI GCTCTTC 1 cut(s) 209
SaqAI TTAA 7 cut(s) 123, 129, 147, 159, 468, 657, 842
SatI GCNGC 2 cut(s) 75, 649
Sau3AI GATC 5 cut(s) 274, 280, 324, 621, 660
Sau96I GGNCC 2 cut(s) 400, 591
ScaI AGTACT 1 cut(s) 809
SchI GAGTC 3 cut(s) 230, 262, 262
SduI GDGCHC 1 cut(s) 250
SfaNI GCATC 3 cut(s) 12, 223, 436
SinI GGWCC 1 cut(s) 591
SmiMI CAYNNNNRTG 2 cut(s) 263, 276
SpeI ACTAGT 1 cut(s) 298
Sse9I AATT 6 cut(s) 54, 65, 124, 184, 378, 391
SsiI CCGC 1 cut(s) 263
SspI AATATT 1 cut(s) 655
SspMI CTAG 6 cut(s) 225, 299, 404, 518, 551, 573
StyI CCWWGG 2 cut(s) 462, 507
TaaI ACNGT 1 cut(s) 502
TaiI ACGT 4 cut(s) 53, 198, 336, 444
TaqI TCGA 1 cut(s) 710
TasI AATT 6 cut(s) 54, 65, 124, 184, 378, 391
TatI WGTACW 1 cut(s) 807
TfiI GAWTC 1 cut(s) 780
Tru1I TTAA 7 cut(s) 123, 129, 147, 159, 468, 657, 842
Tru9I TTAA 7 cut(s) 123, 129, 147, 159, 468, 657, 842
TscAI CASTG 1 cut(s) 255
TseFI GTSAC 2 cut(s) 254, 265
TseI GCWGC 2 cut(s) 74, 648
Tsp45I GTSAC 2 cut(s) 254, 265
TspDTI ATGAA 2 cut(s) 114, 771
TspGWI ACGGA 1 cut(s) 488
TspRI CASTG 1 cut(s) 255
VpaK11BI GGWCC 1 cut(s) 591
XmnI GAANNNNTTC 1 cut(s) 525
XspI CTAG 6 cut(s) 225, 299, 404, 518, 551, 573
ZraI GACGTC 1 cut(s) 51
ZrmI AGTACT 1 cut(s) 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.