MD16G1023800.v1.1

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014728
Physical Location & Seq
Reverse (-)
1721546 .. 1723008
1463 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1023800.v1.1.491

Sequence Viewer

Length: 615 bp
ATGGATGCGCTTCTCTGGACACTGAGTCGCGCCGCAGTGACTCATGATCATGATCAATCACCATTAATAGTCATCGTCCATGTCTTCCCACCGATGCGATTTGTTCCTTCTCCTTGTAATAGGAATGCTTCCAAAAATACAGTGAGTCCAAAGATGGTGAACAATTGCATGGCCCTAGAAAGAGACAGGAGGTGGAAGCTCCTTGACAGATACGTTGATGCCTGCTCATCTGCCAAGGTTAAGACTGAAATTATGCTCGTTGAGAGTGATACAGTTGCCAAGGCACTCCTAGACCTTATTCCTACTCAAAACATAAGAAACCTAGTAGTTGGAACCACCGAATCAAGTCTGAGGAAACTGAGGTCCAAGAAAGGAAGTGGAATAGCCAGTCAGATTCTACGAAATGCGCCCGACACCAGCTGCAATATTAAGATCATATGGAAGGGAAGGGAAGTGATTGATGACAGTATGTTTACTGGCTCGACTTCATCGCGTAGCAGTAACGCAAACTCCTTGTCCACACAAGATGAAGACGATCAGCAAGAAATACGAATCTCAACAGATTACAATAGACAAGAGTACTTGCGTCCTGCAGTAAATCGCCATCATCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

205

Amino Acids

22.99

Weight (kDa)

9.13

Isoelectric Point (pI)

61.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014728)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g32790 FvH4_4g32790
malus_domestica MD13G1022500.v1.1 MD16G1023800.v1.1
prunus_persica Prupe.1G329100_v2.0.a1
pyrus_communis pycom13g02100
rosa_chinensis RchiOBHm_Chr4g0441781
rosa_laevigata RLG00000006066
rosa_multiflora Rmu_sc0013960.1_g000007 Rmu_ssc0000486.1_g000031
rosa_roxburghii Rroxscaffold_5G00382610
rosa_rugosa Rorug04G0336200
rosa_samantha Rh4AG387500 Rh4BG401300 Rh4CG416300 Rh4DG394500
rosa_wichuraiana Rw4G033450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 30, 493
AciI CCGC 1 cut(s) 33
AfaI GTAC 1 cut(s) 581
AhdI GACNNNNNGTC 1 cut(s) 24
AluBI AGCT 2 cut(s) 199, 420
AluI AGCT 2 cut(s) 199, 420
Alw26I GTCTC 1 cut(s) 177
AoxI GGCC 1 cut(s) 171
ApeKI GCWGC 1 cut(s) 420
ArsI GACNNNNNNTTYG 2 cut(s) 500, 532
AseI ATTAAT 1 cut(s) 65
AspLEI GCGC 3 cut(s) 10, 32, 409
AspS9I GGNCC 2 cut(s) 172, 363
AsuHPI GGTGA 2 cut(s) 51, 169
AvaII GGWCC 1 cut(s) 363
BbsI GAAGAC 2 cut(s) 76, 537
BbvI GCAGC 1 cut(s) 407
BccI CCATC 2 cut(s) 148, 612
BclI TGATCA 2 cut(s) 46, 52
BcoDI GTCTC 1 cut(s) 177
BfaI CTAG 3 cut(s) 176, 290, 323
BfmI CTRYAG 1 cut(s) 591
BisI GCNGC 2 cut(s) 33, 421
BlsI GCNGC 2 cut(s) 34, 422
BmcAI AGTACT 1 cut(s) 581
Bme18I GGWCC 1 cut(s) 363
BmeRI GACNNNNNGTC 1 cut(s) 24
BmgT120I GGNCC 2 cut(s) 172, 363
BmiI GGNNCC 1 cut(s) 334
BmsI GCATC 2 cut(s) 84, 208
BpiI GAAGAC 2 cut(s) 76, 537
BsaBI GATNNNNATC 1 cut(s) 51
BsaJI CCNNGG 2 cut(s) 234, 279
BsaXI ACNNNNNCTCC 2 cut(s) 494, 524
Bse1I ACTGG 2 cut(s) 387, 481
Bse8I GATNNNNATC 1 cut(s) 51
BseDI CCNNGG 2 cut(s) 234, 279
BseGI GGATG 1 cut(s) 10
BseJI GATNNNNATC 1 cut(s) 51
BseMII CTCAG 3 cut(s) 14, 341, 350
BseNI ACTGG 2 cut(s) 387, 481
BseXI GCAGC 1 cut(s) 407
Bsh1236I CGCG 2 cut(s) 30, 493
BshFI GGCC 1 cut(s) 173
BsmAI GTCTC 1 cut(s) 177
BsmI GAATGC 1 cut(s) 130
BsnI GGCC 1 cut(s) 173
Bsp143I GATC 4 cut(s) 46, 52, 432, 535
BspACI CCGC 1 cut(s) 33
BspANI GGCC 1 cut(s) 173
BspCNI CTCAG 3 cut(s) 15, 342, 351
BspFNI CGCG 2 cut(s) 30, 493
BspHI TCATGA 2 cut(s) 43, 49
BspLI GGNNCC 1 cut(s) 334
BspMAI CTGCAG 1 cut(s) 595
BsrI ACTGG 2 cut(s) 387, 481
BssECI CCNNGG 2 cut(s) 234, 279
BssMI GATC 4 cut(s) 46, 52, 432, 535
BssT1I CCWWGG 2 cut(s) 234, 279
Bst4CI ACNGT 3 cut(s) 142, 274, 467
BstC8I GCNNGC 1 cut(s) 223
BstDEI CTNAG 3 cut(s) 23, 350, 359
BstF5I GGATG 1 cut(s) 10
BstFNI CGCG 2 cut(s) 30, 493
BstHHI GCGC 3 cut(s) 10, 32, 409
BstKTI GATC 4 cut(s) 49, 55, 435, 538
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 4 cut(s) 46, 52, 432, 535
BstSFI CTRYAG 1 cut(s) 591
BstUI CGCG 2 cut(s) 30, 493
BstV1I GCAGC 1 cut(s) 407
BstV2I GAAGAC 2 cut(s) 76, 537
BsuRI GGCC 1 cut(s) 173
BtgZI GCGATG 1 cut(s) 474
BtsCI GGATG 1 cut(s) 10
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 3 cut(s) 20, 42, 147
Cac8I GCNNGC 1 cut(s) 223
CciI TCATGA 2 cut(s) 43, 49
CfoI GCGC 3 cut(s) 10, 32, 409
Cfr13I GGNCC 2 cut(s) 172, 363
CseI GACGC 1 cut(s) 575
Csp6I GTAC 1 cut(s) 580
CviAII CATG 4 cut(s) 44, 50, 80, 169
CviJI RGCY 5 cut(s) 173, 199, 386, 420, 480
CviKI_1 RGCY 5 cut(s) 173, 199, 386, 420, 480
CviQI GTAC 1 cut(s) 580
DdeI CTNAG 3 cut(s) 23, 350, 359
DpnI GATC 4 cut(s) 48, 54, 434, 537
DpnII GATC 4 cut(s) 46, 52, 432, 535
DriI GACNNNNNGTC 1 cut(s) 24
Eam1105I GACNNNNNGTC 1 cut(s) 24
Eco130I CCWWGG 2 cut(s) 234, 279
Eco47I GGWCC 1 cut(s) 363
EcoT14I CCWWGG 2 cut(s) 234, 279
ErhI CCWWGG 2 cut(s) 234, 279
FaeI CATG 4 cut(s) 47, 53, 83, 172
FaiI YATR 9 cut(s) 45, 51, 81, 170, 254, 314, 437, 439, 470
FatI CATG 4 cut(s) 43, 49, 79, 168
FauNDI CATATG 1 cut(s) 437
FbaI TGATCA 2 cut(s) 46, 52
Fnu4HI GCNGC 2 cut(s) 33, 421
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 2 cut(s) 33, 421
FspBI CTAG 3 cut(s) 176, 290, 323
GlaI GCGC 3 cut(s) 9, 31, 408
GluI GCNGC 2 cut(s) 33, 421
HaeIII GGCC 1 cut(s) 173
HgaI GACGC 1 cut(s) 575
HhaI GCGC 3 cut(s) 10, 32, 409
Hin1II CATG 4 cut(s) 47, 53, 83, 172
Hin6I GCGC 3 cut(s) 8, 30, 407
HinP1I GCGC 3 cut(s) 8, 30, 407
HinfI GANTC 6 cut(s) 25, 40, 145, 341, 394, 552
HphI GGTGA 2 cut(s) 51, 169
Hpy166II GTNNAC 3 cut(s) 160, 474, 519
Hpy188I TCNGA 2 cut(s) 351, 393
Hpy188III TCNNGA 3 cut(s) 16, 44, 50
Hpy8I GTNNAC 3 cut(s) 160, 474, 519
HpyAV CCTTC 3 cut(s) 117, 436, 441
HpyCH4III ACNGT 3 cut(s) 142, 274, 467
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 3 cut(s) 168, 423, 593
HpyF3I CTNAG 3 cut(s) 23, 350, 359
HpySE526I ACGT 1 cut(s) 213
Hsp92II CATG 4 cut(s) 47, 53, 83, 172
HspAI GCGC 3 cut(s) 8, 30, 407
Ksp22I TGATCA 2 cut(s) 46, 52
Kzo9I GATC 4 cut(s) 46, 52, 432, 535
LmnI GCTCC 1 cut(s) 204
LpnPI CCDG 6 cut(s) 172, 235, 400, 430, 462, 603
Lsp1109I GCAGC 1 cut(s) 407
LweI GCATC 2 cut(s) 84, 208
MaeI CTAG 3 cut(s) 176, 290, 323
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 2 cut(s) 37, 500
MalI GATC 4 cut(s) 48, 54, 434, 537
MboI GATC 4 cut(s) 46, 52, 432, 535
MboII GAAGA 2 cut(s) 76, 542
MfeI CAATTG 1 cut(s) 163
MluCI AATT 2 cut(s) 163, 249
MlyI GAGTC 3 cut(s) 34, 34, 154
MmeI TCCRAC 1 cut(s) 310
MnlI CCTC 3 cut(s) 183, 345, 354
MseI TTAA 4 cut(s) 65, 240, 429, 613
MslI CAYNNNNRTG 1 cut(s) 48
MspA1I CMGCKG 1 cut(s) 420
MunI CAATTG 1 cut(s) 163
Mva1269I GAATGC 1 cut(s) 130
MvnI CGCG 2 cut(s) 30, 493
NdeI CATATG 1 cut(s) 437
NdeII GATC 4 cut(s) 46, 52, 432, 535
NlaIII CATG 4 cut(s) 47, 53, 83, 172
NlaIV GGNNCC 1 cut(s) 334
NmuCI GTSAC 1 cut(s) 37
PagI TCATGA 2 cut(s) 43, 49
PctI GAATGC 1 cut(s) 130
PfeI GAWTC 3 cut(s) 341, 394, 552
PkrI GCNGC 2 cut(s) 34, 422
PleI GAGTC 3 cut(s) 33, 34, 153
PpsI GAGTC 3 cut(s) 33, 34, 153
PshBI ATTAAT 1 cut(s) 65
PspN4I GGNNCC 1 cut(s) 334
PspPI GGNCC 2 cut(s) 172, 363
PstI CTGCAG 1 cut(s) 595
PvuII CAGCTG 1 cut(s) 420
RsaI GTAC 1 cut(s) 581
RsaNI GTAC 1 cut(s) 580
RseI CAYNNNNRTG 1 cut(s) 48
SaqAI TTAA 4 cut(s) 65, 240, 429, 613
SatI GCNGC 2 cut(s) 33, 421
Sau3AI GATC 4 cut(s) 46, 52, 432, 535
Sau96I GGNCC 2 cut(s) 172, 363
ScaI AGTACT 1 cut(s) 581
SchI GAGTC 3 cut(s) 34, 34, 154
SetI ASST 8 cut(s) 194, 201, 216, 240, 297, 324, 365, 422
SfaNI GCATC 2 cut(s) 84, 208
SfcI CTRYAG 1 cut(s) 591
SinI GGWCC 1 cut(s) 363
SmiMI CAYNNNNRTG 1 cut(s) 48
Sse9I AATT 2 cut(s) 163, 249
SsiI CCGC 1 cut(s) 33
SspI AATATT 1 cut(s) 427
SspMI CTAG 3 cut(s) 176, 290, 323
StyI CCWWGG 2 cut(s) 234, 279
TaaI ACNGT 3 cut(s) 142, 274, 467
TaiI ACGT 1 cut(s) 216
TaqI TCGA 1 cut(s) 482
TasI AATT 2 cut(s) 163, 249
TatI WGTACW 1 cut(s) 579
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 3 cut(s) 341, 394, 552
Tru1I TTAA 4 cut(s) 65, 240, 429, 613
Tru9I TTAA 4 cut(s) 65, 240, 429, 613
TscAI CASTG 3 cut(s) 27, 42, 147
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 1 cut(s) 420
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 2 cut(s) 477, 543
TspRI CASTG 3 cut(s) 27, 42, 147
VpaK11BI GGWCC 1 cut(s) 363
VspI ATTAAT 1 cut(s) 65
XspI CTAG 3 cut(s) 176, 290, 323
ZrmI AGTACT 1 cut(s) 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.