Prupe.1G329100_v2.0.a1

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0014728
Physical Location & Seq
Forward (+)
31266012 .. 31268447
2436 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G329100.1

Sequence Viewer

Length: 1026 bp
ATGACGACGGAAGAGATGATGATGATGACAAGTTCAAGCAACTTGTTTGAGAACAGCAGCAGTTATGGCATGCCCCTGCCCTTGTCATCCATCGAAGAAGAAGAAGAAGAAGAAGAAGAAGAGGAGTCTACAATCTCTGTCTCTGATGATGATGATGATGATGCAGAAAGTCTTTTCTCCATTGATTTTAATATGAATCATAATAATATGCATAATAGTTATAAGAAGGAGGAGGAGGATGACAGGGAAGTTTGTTATTATCATTATTATGTAGCAGTGGGGAGGAAGAGCGAGTCTAGCATGGATGCCCTTCTCTGGACGGTTAACCACGCAGCTACTCCTCAATCATCATCCACTTCACTAGTCAATGTAGTCATCGTCCATGTCTTTCCCCCTCTTCAATTCATTCCTTCTCCATTAGGAATGCTTCCAAAAAGTAAAGTGAGCCCAAAGATGGTGGACAAGTACATGGTCCAAGAAAGAGACAGAAGGAGAAAGCTCCTTGAGAAATACGTTGATGCCTGTTCAGCTGCCAAGGTCAAGGTGGATGTTATGCTTATTGAGAGTGATACGGTTTCCAAGGCTATCCTAGACCTAATTCCAACTCAAAACATCAAAACTCTTGTTGTTGGAACCACCAATTCCAGTATGAGGAAATTGAGGTCTAAGAAAGGAAGTGGGATTGCTAGTCAGATTCTACGAAATGCACCTGAAACAAGCTGCGAAATTCGGATCATATGCAAGGGCAAGGAAGTGATGGATCAGGATCACACGATTACAGGGTCAATTTCCTCACGTAGCAGCAATGCGAATTCCTTGTCTACGCAAGAGGAAGATGATAATCAAGAACTACCAATCTCACCAGATTATAATAAGCAAGGGCTGCATTACACGCTTCCTGCAGTGAAATCTCCATCAGCAATTCCGGACACTACTACTTCTGCTCCACTTCTGTCTTTAAATGGATACAAGGCAATGTGGAGGGGAAAACTAAAACCAAAAAAACAGCTTGATTTTGCTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

342

Amino Acids

38.13

Weight (kDa)

5.0

Isoelectric Point (pI)

67.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014728)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g32790 FvH4_4g32790
malus_domestica MD13G1022500.v1.1 MD16G1023800.v1.1
prunus_persica Prupe.1G329100_v2.0.a1
pyrus_communis pycom13g02100
rosa_chinensis RchiOBHm_Chr4g0441781
rosa_laevigata RLG00000006066
rosa_multiflora Rmu_sc0013960.1_g000007 Rmu_ssc0000486.1_g000031
rosa_roxburghii Rroxscaffold_5G00382610
rosa_rugosa Rorug04G0336200
rosa_samantha Rh4AG387500 Rh4BG401300 Rh4CG416300 Rh4DG394500
rosa_wichuraiana Rw4G033450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 222, 870
AccI GTMKAC 2 cut(s) 128, 821
AccIII TCCGGA 1 cut(s) 925
AclWI GGATC 3 cut(s) 740, 768, 774
AcsI RAATTY 2 cut(s) 726, 811
AfaI GTAC 1 cut(s) 467
AfiI CCNNNNNNNGG 3 cut(s) 315, 454, 651
AgsI TTSAA 2 cut(s) 36, 401
AhlI ACTAGT 1 cut(s) 361
AluBI AGCT 5 cut(s) 335, 499, 530, 720, 1009
AluI AGCT 5 cut(s) 335, 499, 530, 720, 1009
Alw26I GTCTC 2 cut(s) 145, 477
AlwI GGATC 3 cut(s) 740, 768, 774
Aor13HI TCCGGA 1 cut(s) 925
ApeKI GCWGC 6 cut(s) 57, 332, 530, 720, 801, 883
ApoI RAATTY 2 cut(s) 726, 811
ArsI GACNNNNNNTTYG 2 cut(s) 803, 835
AspS9I GGNCC 1 cut(s) 472
AsuHPI GGTGA 1 cut(s) 852
AvaII GGWCC 1 cut(s) 472
BanII GRGCYC 1 cut(s) 449
BbvI GCAGC 6 cut(s) 69, 344, 517, 707, 813, 870
BccI CCATC 4 cut(s) 98, 448, 751, 922
BciVI GTATCC 1 cut(s) 959
BcoDI GTCTC 2 cut(s) 145, 477
BcuI ACTAGT 1 cut(s) 361
BfaI CTAG 4 cut(s) 297, 362, 590, 687
BfmI CTRYAG 1 cut(s) 900
BfuI GTATCC 1 cut(s) 959
BisI GCNGC 6 cut(s) 58, 333, 531, 721, 802, 884
BlsI GCNGC 6 cut(s) 59, 334, 532, 722, 803, 885
Bme18I GGWCC 1 cut(s) 472
BmgT120I GGNCC 1 cut(s) 472
BmiI GGNNCC 1 cut(s) 634
BmsI GCATC 3 cut(s) 151, 295, 508
BpuEI CTTGAG 1 cut(s) 524
BsaAI YACGTR 1 cut(s) 797
BsaBI GATNNNNATC 2 cut(s) 765, 840
BsaJI CCNNGG 2 cut(s) 534, 579
BsaWI WCCGGW 1 cut(s) 925
BsaXI ACNNNNNCTCC 2 cut(s) 928, 958
Bsc4I CCNNNNNNNGG 3 cut(s) 315, 454, 651
Bse1I ACTGG 1 cut(s) 645
Bse3DI GCAATG 2 cut(s) 811, 981
Bse8I GATNNNNATC 2 cut(s) 765, 840
BseAI TCCGGA 1 cut(s) 925
BseDI CCNNGG 2 cut(s) 534, 579
BseGI GGATG 5 cut(s) 86, 244, 310, 350, 553
BseJI GATNNNNATC 2 cut(s) 765, 840
BseLI CCNNNNNNNGG 3 cut(s) 315, 454, 651
BseMI GCAATG 2 cut(s) 811, 981
BseNI ACTGG 1 cut(s) 645
BseRI GAGGAG 4 cut(s) 137, 245, 248, 330
BseXI GCAGC 6 cut(s) 69, 344, 517, 707, 813, 870
BsiSI CCGG 1 cut(s) 926
BslI CCNNNNNNNGG 3 cut(s) 315, 454, 651
BsmAI GTCTC 2 cut(s) 145, 477
BsmI GAATGC 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 449
Bsp13I TCCGGA 1 cut(s) 925
Bsp143I GATC 3 cut(s) 732, 760, 766
BspEI TCCGGA 1 cut(s) 925
BspLI GGNNCC 1 cut(s) 634
BspMAI CTGCAG 1 cut(s) 904
BspPI GGATC 3 cut(s) 740, 768, 774
BspQI GCTCTTC 1 cut(s) 281
BsrDI GCAATG 2 cut(s) 811, 981
BsrI ACTGG 1 cut(s) 645
BssECI CCNNGG 2 cut(s) 534, 579
BssMI GATC 3 cut(s) 732, 760, 766
BssT1I CCWWGG 2 cut(s) 534, 579
Bst4CI ACNGT 2 cut(s) 322, 574
Bst6I CTCTTC 4 cut(s) 6, 114, 281, 402
BstAPI GCANNNNNTGC 1 cut(s) 883
BstBAI YACGTR 1 cut(s) 797
BstC8I GCNNGC 1 cut(s) 71
BstDEI CTNAG 1 cut(s) 666
BstF5I GGATG 5 cut(s) 86, 244, 310, 350, 553
BstKTI GATC 3 cut(s) 735, 763, 769
BstMAI GTCTC 2 cut(s) 145, 477
BstMBI GATC 3 cut(s) 732, 760, 766
BstMWI GCNNNNNNNGC 5 cut(s) 66, 297, 527, 883, 892
BstNSI RCATGY 1 cut(s) 73
BstSFI CTRYAG 1 cut(s) 900
BstV1I GCAGC 6 cut(s) 69, 344, 517, 707, 813, 870
BsuI GTATCC 1 cut(s) 959
BtsCI GGATG 5 cut(s) 86, 244, 310, 350, 553
BtsI GCAGTG 2 cut(s) 282, 909
BtsIMutI CAGTG 2 cut(s) 282, 909
Cac8I GCNNGC 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 472
Csp6I GTAC 1 cut(s) 466
CspCI CAANNNNNGTGG 2 cut(s) 438, 473
CviAII CATG 4 cut(s) 70, 301, 383, 469
CviJI RGCY 8 cut(s) 335, 447, 499, 530, 584, 720, 883, 1009
CviKI_1 RGCY 8 cut(s) 335, 447, 499, 530, 584, 720, 883, 1009
CviQI GTAC 1 cut(s) 466
DdeI CTNAG 1 cut(s) 666
DpnI GATC 3 cut(s) 734, 762, 768
DpnII GATC 3 cut(s) 732, 760, 766
DraI TTTAAA 1 cut(s) 960
Eam1104I CTCTTC 4 cut(s) 6, 114, 281, 402
EarI CTCTTC 4 cut(s) 6, 114, 281, 402
Eco130I CCWWGG 2 cut(s) 534, 579
Eco24I GRGCYC 1 cut(s) 449
Eco47I GGWCC 1 cut(s) 472
EcoRI GAATTC 1 cut(s) 811
EcoT14I CCWWGG 2 cut(s) 534, 579
EcoT22I ATGCAT 1 cut(s) 213
EcoT38I GRGCYC 1 cut(s) 449
ErhI CCWWGG 2 cut(s) 534, 579
FaeI CATG 4 cut(s) 73, 304, 386, 472
FatI CATG 4 cut(s) 69, 300, 382, 468
FauNDI CATATG 1 cut(s) 737
FblI GTMKAC 2 cut(s) 128, 821
Fnu4HI GCNGC 6 cut(s) 58, 333, 531, 721, 802, 884
FokI GGATG 5 cut(s) 73, 251, 317, 337, 560
FriOI GRGCYC 1 cut(s) 449
Fsp4HI GCNGC 6 cut(s) 58, 333, 531, 721, 802, 884
FspBI CTAG 4 cut(s) 297, 362, 590, 687
GluI GCNGC 6 cut(s) 58, 333, 531, 721, 802, 884
HapII CCGG 1 cut(s) 926
Hin1II CATG 4 cut(s) 73, 304, 386, 472
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HinfI GANTC 4 cut(s) 125, 196, 293, 694
HpaI GTTAAC 1 cut(s) 325
HpaII CCGG 1 cut(s) 926
HphI GGTGA 1 cut(s) 852
Hpy166II GTNNAC 4 cut(s) 129, 325, 460, 822
Hpy188I TCNGA 3 cut(s) 145, 693, 732
Hpy188III TCNNGA 4 cut(s) 316, 764, 845, 926
Hpy8I GTNNAC 4 cut(s) 129, 325, 460, 822
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 4 cut(s) 220, 320, 420, 483
HpyCH4III ACNGT 2 cut(s) 322, 574
HpyCH4IV ACGT 2 cut(s) 513, 796
HpyCH4V TGCA 6 cut(s) 164, 211, 707, 741, 886, 902
HpyF10VI GCNNNNNNNGC 5 cut(s) 66, 297, 527, 883, 892
HpyF3I CTNAG 1 cut(s) 666
HpySE526I ACGT 2 cut(s) 513, 796
Hsp92II CATG 4 cut(s) 73, 304, 386, 472
Kpn2I TCCGGA 1 cut(s) 925
KspAI GTTAAC 1 cut(s) 325
Kzo9I GATC 3 cut(s) 732, 760, 766
LguI GCTCTTC 1 cut(s) 281
LmnI GCTCC 2 cut(s) 504, 949
Lsp1109I GCAGC 6 cut(s) 69, 344, 517, 707, 813, 870
LweI GCATC 3 cut(s) 151, 295, 508
MaeI CTAG 4 cut(s) 297, 362, 590, 687
MaeII ACGT 2 cut(s) 513, 796
MalI GATC 3 cut(s) 734, 762, 768
MboI GATC 3 cut(s) 732, 760, 766
MhlI GDGCHC 1 cut(s) 449
MluCI AATT 8 cut(s) 401, 597, 640, 656, 726, 786, 811, 921
MlyI GAGTC 2 cut(s) 134, 302
MmeI TCCRAC 2 cut(s) 610, 626
Mph1103I ATGCAT 1 cut(s) 213
MroI TCCGGA 1 cut(s) 925
MseI TTAA 3 cut(s) 189, 324, 959
MslI CAYNNNNRTG 1 cut(s) 267
MspA1I CMGCKG 1 cut(s) 530
MspI CCGG 1 cut(s) 926
Mva1269I GAATGC 1 cut(s) 429
MwoI GCNNNNNNNGC 5 cut(s) 66, 297, 527, 883, 892
NdeI CATATG 1 cut(s) 737
NdeII GATC 3 cut(s) 732, 760, 766
NlaIII CATG 4 cut(s) 73, 304, 386, 472
NlaIV GGNNCC 1 cut(s) 634
NsiI ATGCAT 1 cut(s) 213
NspI RCATGY 1 cut(s) 73
PaeI GCATGC 1 cut(s) 73
PciSI GCTCTTC 1 cut(s) 281
PctI GAATGC 1 cut(s) 429
PfeI GAWTC 2 cut(s) 196, 694
PkrI GCNGC 6 cut(s) 59, 334, 532, 722, 803, 885
PleI GAGTC 2 cut(s) 133, 301
PpsI GAGTC 2 cut(s) 133, 301
Ppu21I YACGTR 1 cut(s) 797
PsiI TTATAA 2 cut(s) 222, 870
PspN4I GGNNCC 1 cut(s) 634
PspPI GGNCC 1 cut(s) 472
PstI CTGCAG 1 cut(s) 904
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 1 cut(s) 467
RsaNI GTAC 1 cut(s) 466
RseI CAYNNNNRTG 1 cut(s) 267
SapI GCTCTTC 1 cut(s) 281
SaqAI TTAA 3 cut(s) 189, 324, 959
SatI GCNGC 6 cut(s) 58, 333, 531, 721, 802, 884
Sau3AI GATC 3 cut(s) 732, 760, 766
Sau96I GGNCC 1 cut(s) 472
SchI GAGTC 2 cut(s) 134, 302
SduI GDGCHC 1 cut(s) 449
SfaNI GCATC 3 cut(s) 151, 295, 508
SfcI CTRYAG 1 cut(s) 900
SinI GGWCC 1 cut(s) 472
SmiMI CAYNNNNRTG 1 cut(s) 267
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
SpeI ACTAGT 1 cut(s) 361
SphI GCATGC 1 cut(s) 73
Sse9I AATT 8 cut(s) 401, 597, 640, 656, 726, 786, 811, 921
SspMI CTAG 4 cut(s) 297, 362, 590, 687
StyI CCWWGG 2 cut(s) 534, 579
TaaI ACNGT 2 cut(s) 322, 574
TaiI ACGT 2 cut(s) 516, 799
TaqI TCGA 1 cut(s) 93
TasI AATT 8 cut(s) 401, 597, 640, 656, 726, 786, 811, 921
TatI WGTACW 1 cut(s) 465
TfiI GAWTC 2 cut(s) 196, 694
Tru1I TTAA 3 cut(s) 189, 324, 959
Tru9I TTAA 3 cut(s) 189, 324, 959
TscAI CASTG 2 cut(s) 282, 909
TseI GCWGC 6 cut(s) 57, 332, 530, 720, 801, 883
TspDTI ATGAA 2 cut(s) 209, 394
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 2 cut(s) 282, 909
VpaK11BI GGWCC 1 cut(s) 472
XapI RAATTY 2 cut(s) 726, 811
XceI RCATGY 1 cut(s) 73
XcmI CCANNNNNNNNNTGG 1 cut(s) 541
XmiI GTMKAC 2 cut(s) 128, 821
XspI CTAG 4 cut(s) 297, 362, 590, 687
Zsp2I ATGCAT 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.