Rorug04G0336200

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0014728
Physical Location & Seq
Forward (+)
51012021 .. 51014145
2125 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0336200.1

Sequence Viewer

Length: 1785 bp
ATGGCATCTGGTTTCTATGTGCAACCTTGTGAAGTCTATGGCAATAACAAATGTGATGAGGTTATTGGTCTTGATCTCAATCTCTCATCCATGTCATGCCTTCCCTTCCCTTCTCTTTCGACTCTGGAGAACAATTCTGCTGTCTGGGAGATTCCGTTCATAGATGAAACAAGAAATCATAAGAGGGTAAAGCTTGAAGATAGTGCCGATGAATCCATTATAAGTACTAATTATAGTCTTTACAGTGGCAGTGCCAGTGGCAGCATGTATACTAGTGGCTTCAATAGCTTGTCAAGAATTCAATTCCGGGATCACATATCAGCTTACACCCGGAGATACTTGGCAGCAGAGAAAATGGAAGAAGCAGCTTCAACCTTGCTGGGAGGCGAGCCCGAGGAAGAAGGAAGTGAAGATGGAATGAAACTAGTTCAAAAGCTCATTTCGTGCGCTGAGGCTGTGGCTTGTCGTGACAAAACTCATGCTTCAGCATTGCTTTCTGAGCTTAGAACTAATGCCCAGGTCTTTGGGACATCATTCCAGCGAGTAGCTTCCTGCTTTATCCAAGCTCTCTCTGACCGTCTTGCTCTCGTTCAACCACTGGGGGCTGTGGGAGTTACAACAAAGATGACTGCTGTTTCATCAGAGAAGGATGAGGCCTTGCACCTTGTTTATGAGATTTGTCCACATATCCGGTTTAGTCATTTTGTAGCAAACACATCAATATCGGAAGCCTTTGAGGGGGAGAGCTATGTTCATGTCATAGACTTGGGCATGACCCTAGGCCTCCTTCATGGCTACCAATGGCTCAACTTGATGGAAAGTCTAGCCAGCCGAACGGGACAACCCTTACGCCGGCTTCGAATAACTGGTGTTGGTGACTCTGGTGAACGCCTTGAAGCGATTGGTAATGAACTAAAGTTGTATGCACAAAGCATGGAGCTGGATTTTGGATTTTTAGCAGTGGAAAGCAACTTGGAAAACCTGAAGGCCGAAGATTTCAATCTCATTGATGGGGAGGTTTTAGTCATCAATAGCATACTCCAGCTGCATTGTCTTGTGAAAGAGAGTAGAGGAGCATTGAACTCGGTTCTACAAACACTATATGACCTATCACCAAAGCTAATGGTTCTAGTTGAGCAGGACTCGAGCCACAATGGGCCTTTCTTTCTTGGGAGGTTCATGGAGGCATTACATAATTACTCTGCCATCTTCGACTCCTTAGATGCAATGCTGCCCAGATATGACACAAGGAGAGTTAAAATGGAGCAGTTTTATTTCGGAGAGGAGATTAAGAACATAGTAAGCTGCGAGGGACCTGCGCGAGTGGAGCGGCACGAAAGAGTTGATCAATGGCGCAGGAGGATGAGGCGCGCTGGGTTCCAACCGGCACCCCTGAAGATGATTACGCAGGCTACAAAATGGCTAGAAATGAATGCTAGTGAGGGTTACACAATTGTGGAAGACAAGGGTTGCTTTGTTCTTGGGTGGAAGTCGAAGCCTATCATTGCAGCTTCTTGCTGGAGATGTTATGGTGCCATCTTTGGAGATTTGAGAAGTGGTATTATGGATGTCTTTTGCTCTAATCTTAATATTCCGAGCTCTGTGACAGCGAAGATGCATTTCCGTTCTTCACATTTCTATCGTGAAGGAAACCAAGCTGCAGATGCTCTTGCTAACATAGGTCTCTATTCTGATAGTTTAACGTGGTGGGACAGGCCCCCGGATCTTACTCGCTCTTATTGTCAGAAGGATTACTTGGGGTTGCCCAATTTTCGTTTTCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

594

Amino Acids

66.67

Weight (kDa)

5.51

Isoelectric Point (pI)

50.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 142 - 508 8.1e-111 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014728)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g32790 FvH4_4g32790
malus_domestica MD13G1022500.v1.1 MD16G1023800.v1.1
prunus_persica Prupe.1G329100_v2.0.a1
pyrus_communis pycom13g02100
rosa_chinensis RchiOBHm_Chr4g0441781
rosa_laevigata RLG00000006066
rosa_multiflora Rmu_sc0013960.1_g000007 Rmu_ssc0000486.1_g000031
rosa_roxburghii Rroxscaffold_5G00382610
rosa_rugosa Rorug04G0336200
rosa_samantha Rh4AG387500 Rh4BG401300 Rh4CG416300 Rh4DG394500
rosa_wichuraiana Rw4G033450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 221
Acc36I ACCTGC 1 cut(s) 1324
AccB1I GGYRCC 2 cut(s) 1387, 1532
AccBSI CCGCTC 1 cut(s) 1330
AccI GTMKAC 1 cut(s) 269
AccII CGCG 2 cut(s) 1321, 1371
AciI CCGC 1 cut(s) 1330
AclWI GGATC 2 cut(s) 318, 1731
AcsI RAATTY 1 cut(s) 297
AcuI CTGAAG 3 cut(s) 468, 1004, 1415
AfaI GTAC 1 cut(s) 226
AfiI CCNNNNNNNGG 2 cut(s) 738, 852
AgsI TTSAA 9 cut(s) 197, 283, 302, 372, 431, 593, 896, 1000, 1081
AhlI ACTAGT 2 cut(s) 272, 424
AjnI CCWGG 1 cut(s) 516
AjuI GAANNNNNNNTTGG 2 cut(s) 1739, 1771
AleI CACNNNNGTG 1 cut(s) 1454
AloI GAACNNNNNNTCC 2 cut(s) 140, 172
Alw21I GWGCWC 1 cut(s) 1601
Alw26I GTCTC 1 cut(s) 1688
AlwI GGATC 2 cut(s) 318, 1731
Ama87I CYCGRG 2 cut(s) 392, 1144
AoxI GGCC 5 cut(s) 654, 781, 987, 1157, 1715
ApeKI GCWGC 8 cut(s) 261, 344, 365, 1045, 1231, 1305, 1508, 1658
ApoI RAATTY 1 cut(s) 297
AspA2I CCTAGG 1 cut(s) 778
AspLEI GCGC 5 cut(s) 449, 1321, 1356, 1371, 1373
AspS9I GGNCC 3 cut(s) 1157, 1313, 1716
AsuC2I CCSGG 3 cut(s) 308, 331, 1721
AsuHPI GGTGA 3 cut(s) 887, 896, 1104
AsuII TTCGAA 1 cut(s) 859
AvaI CYCGRG 2 cut(s) 392, 1144
AvaII GGWCC 1 cut(s) 1313
AvrII CCTAGG 1 cut(s) 778
BanI GGYRCC 2 cut(s) 1387, 1532
BanII GRGCYC 2 cut(s) 393, 1601
BbsI GAAGAC 1 cut(s) 1467
Bbv12I GWGCWC 1 cut(s) 1601
BbvCI CCTCAGC 1 cut(s) 450
BbvI GCAGC 8 cut(s) 273, 356, 377, 1032, 1218, 1292, 1520, 1645
BccI CCATC 5 cut(s) 407, 808, 1004, 1214, 1544
BcgI CGANNNNNNTGC 4 cut(s) 1065, 1099, 1298, 1332
BciT130I CCWGG 1 cut(s) 518
BclI TGATCA 1 cut(s) 1345
BcnI CCSGG 3 cut(s) 308, 331, 1721
BcoDI GTCTC 1 cut(s) 1688
BcuI ACTAGT 2 cut(s) 272, 424
BfaI CTAG 7 cut(s) 273, 425, 779, 824, 1130, 1424, 1437
BfmI CTRYAG 1 cut(s) 1659
BfuAI ACCTGC 1 cut(s) 1324
BisI GCNGC 9 cut(s) 262, 345, 366, 1046, 1232, 1306, 1331, 1509, 1659
BlnI CCTAGG 1 cut(s) 778
BlsI GCNGC 9 cut(s) 263, 346, 367, 1047, 1233, 1307, 1332, 1510, 1660
BmcAI AGTACT 1 cut(s) 226
Bme1390I CCNGG 4 cut(s) 308, 331, 518, 1721
Bme18I GGWCC 1 cut(s) 1313
BmeT110I CYCGRG 2 cut(s) 392, 1144
BmgT120I GGNCC 3 cut(s) 1157, 1313, 1716
BmiI GGNNCC 5 cut(s) 1314, 1379, 1389, 1534, 1718
BmrFI CCNGG 4 cut(s) 308, 331, 518, 1721
BmrI ACTGGG 1 cut(s) 608
BmsI GCATC 4 cut(s) 14, 1213, 1605, 1654
BmuI ACTGGG 1 cut(s) 608
BpiI GAAGAC 1 cut(s) 1467
BplI GAGNNNNNCTC 2 cut(s) 1127, 1159
BpmI CTGGAG 3 cut(s) 146, 1025, 1540
Bpu10I CCTNAGC 1 cut(s) 450
Bpu14I TTCGAA 1 cut(s) 859
BpuMI CCSGG 3 cut(s) 308, 331, 1721
BsaBI GATNNNNATC 2 cut(s) 78, 999
BsaI GGTCTC 1 cut(s) 1688
BsaJI CCNNGG 4 cut(s) 393, 516, 778, 1719
BsaWI WCCGGW 1 cut(s) 690
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 2 cut(s) 738, 852
Bse118I RCCGGY 2 cut(s) 852, 1384
Bse1I ACTGG 3 cut(s) 255, 603, 871
Bse3DI GCAATG 3 cut(s) 488, 1233, 1503
Bse8I GATNNNNATC 2 cut(s) 78, 999
BseBI CCWGG 1 cut(s) 518
BseDI CCNNGG 4 cut(s) 393, 516, 778, 1719
BseGI GGATG 4 cut(s) 86, 655, 1368, 1573
BseJI GATNNNNATC 2 cut(s) 78, 999
BseLI CCNNNNNNNGG 2 cut(s) 738, 852
BseMI GCAATG 3 cut(s) 488, 1233, 1503
BseMII CTCAG 2 cut(s) 441, 489
BseNI ACTGG 3 cut(s) 255, 603, 871
BsePI GCGCGC 1 cut(s) 1369
BseRI GAGGAG 2 cut(s) 1086, 1298
BseXI GCAGC 8 cut(s) 273, 356, 377, 1032, 1218, 1292, 1520, 1645
BseYI CCCAGC 2 cut(s) 379, 1373
Bsh1236I CGCG 2 cut(s) 1321, 1371
BshFI GGCC 5 cut(s) 656, 783, 989, 1159, 1717
BshNI GGYRCC 2 cut(s) 1387, 1532
BsiHKAI GWGCWC 1 cut(s) 1601
BsiHKCI CYCGRG 2 cut(s) 392, 1144
BsiSI CCGG 6 cut(s) 307, 331, 691, 853, 1385, 1721
BslFI GGGAC 4 cut(s) 541, 852, 1326, 1724
BslI CCNNNNNNNGG 2 cut(s) 738, 852
BsmAI GTCTC 1 cut(s) 1688
BsmFI GGGAC 4 cut(s) 541, 852, 1326, 1724
BsmI GAATGC 1 cut(s) 1438
BsnI GGCC 5 cut(s) 656, 783, 989, 1159, 1717
Bso31I GGTCTC 1 cut(s) 1688
BsoBI CYCGRG 2 cut(s) 392, 1144
Bsp119I TTCGAA 1 cut(s) 859
Bsp1286I GDGCHC 2 cut(s) 393, 1601
Bsp143I GATC 4 cut(s) 73, 310, 1345, 1723
BspACI CCGC 1 cut(s) 1330
BspANI GGCC 5 cut(s) 656, 783, 989, 1159, 1717
BspCNI CTCAG 2 cut(s) 442, 490
BspFNI CGCG 2 cut(s) 1321, 1371
BspLI GGNNCC 5 cut(s) 1314, 1379, 1389, 1534, 1718
BspMAI CTGCAG 1 cut(s) 1663
BspMI ACCTGC 1 cut(s) 1324
BspPI GGATC 2 cut(s) 318, 1731
BspT104I TTCGAA 1 cut(s) 859
BspT107I GGYRCC 2 cut(s) 1387, 1532
BspTNI GGTCTC 1 cut(s) 1688
BsrBI CCGCTC 1 cut(s) 1330
BsrDI GCAATG 3 cut(s) 488, 1233, 1503
BsrFI RCCGGY 2 cut(s) 852, 1384
BsrI ACTGG 3 cut(s) 255, 603, 871
BssAI RCCGGY 2 cut(s) 852, 1384
BssECI CCNNGG 4 cut(s) 393, 516, 778, 1719
BssHII GCGCGC 1 cut(s) 1369
BssMI GATC 4 cut(s) 73, 310, 1345, 1723
BssNAI GTATAC 1 cut(s) 270
BssT1I CCWWGG 1 cut(s) 778
Bst1107I GTATAC 1 cut(s) 270
Bst2UI CCWGG 1 cut(s) 518
Bst4CI ACNGT 2 cut(s) 245, 578
BstBI TTCGAA 1 cut(s) 859
BstC8I GCNNGC 5 cut(s) 389, 829, 854, 1371, 1410
BstDEI CTNAG 4 cut(s) 450, 498, 503, 1219
BstF5I GGATG 4 cut(s) 86, 655, 1368, 1573
BstFNI CGCG 2 cut(s) 1321, 1371
BstHHI GCGC 5 cut(s) 449, 1321, 1356, 1371, 1373
BstKTI GATC 4 cut(s) 76, 313, 1348, 1726
BstMAI GTCTC 1 cut(s) 1688
BstMBI GATC 4 cut(s) 73, 310, 1345, 1723
BstMWI GCNNNNNNNGC 4 cut(s) 285, 499, 1327, 1664
BstNI CCWGG 1 cut(s) 518
BstNSI RCATGY 1 cut(s) 268
BstSCI CCNGG 4 cut(s) 306, 329, 516, 1719
BstSFI CTRYAG 1 cut(s) 1659
BstUI CGCG 2 cut(s) 1321, 1371
BstV1I GCAGC 8 cut(s) 273, 356, 377, 1032, 1218, 1292, 1520, 1645
BstV2I GAAGAC 1 cut(s) 1467
BstX2I RGATCY 1 cut(s) 1723
BstXI CCANNNNNNTGG 1 cut(s) 524
BstYI RGATCY 1 cut(s) 1723
BstZ17I GTATAC 1 cut(s) 270
BsuRI GGCC 5 cut(s) 656, 783, 989, 1159, 1717
BtsCI GGATG 4 cut(s) 86, 655, 1368, 1573
BtsI GCAGTG 2 cut(s) 256, 966
BtsIMutI CAGTG 5 cut(s) 250, 256, 262, 596, 966
BveI ACCTGC 1 cut(s) 1324
Cac8I GCNNGC 5 cut(s) 389, 829, 854, 1371, 1410
CfoI GCGC 5 cut(s) 449, 1321, 1356, 1371, 1373
Cfr10I RCCGGY 2 cut(s) 852, 1384
Cfr13I GGNCC 3 cut(s) 1157, 1313, 1716
Csp6I GTAC 1 cut(s) 225
CviAII CATG 9 cut(s) 91, 96, 265, 479, 755, 772, 791, 934, 1180
CviQI GTAC 1 cut(s) 225
DdeI CTNAG 4 cut(s) 450, 498, 503, 1219
DpnI GATC 4 cut(s) 75, 312, 1347, 1725
DpnII GATC 4 cut(s) 73, 310, 1345, 1723
Ecl136II GAGCTC 1 cut(s) 1599
Eco130I CCWWGG 1 cut(s) 778
Eco147I AGGCCT 2 cut(s) 656, 783
Eco24I GRGCYC 2 cut(s) 393, 1601
Eco31I GGTCTC 1 cut(s) 1688
Eco47I GGWCC 1 cut(s) 1313
Eco53kI GAGCTC 1 cut(s) 1599
Eco57I CTGAAG 3 cut(s) 468, 1004, 1415
Eco88I CYCGRG 2 cut(s) 392, 1144
EcoICRI GAGCTC 1 cut(s) 1599
EcoO109I RGGNCCY 2 cut(s) 1313, 1716
EcoRI GAATTC 1 cut(s) 297
EcoRII CCWGG 1 cut(s) 516
EcoT14I CCWWGG 1 cut(s) 778
EcoT22I ATGCAT 1 cut(s) 1620
EcoT38I GRGCYC 2 cut(s) 393, 1601
ErhI CCWWGG 1 cut(s) 778
FaeI CATG 9 cut(s) 94, 99, 268, 482, 758, 775, 794, 937, 1183
FalI AAGNNNNNCTT 4 cut(s) 1457, 1489, 1739, 1771
FaqI GGGAC 4 cut(s) 541, 852, 1326, 1724
FatI CATG 9 cut(s) 90, 95, 264, 478, 754, 771, 790, 933, 1179
FbaI TGATCA 1 cut(s) 1345
FblI GTMKAC 1 cut(s) 269
Fnu4HI GCNGC 9 cut(s) 262, 345, 366, 1046, 1232, 1306, 1331, 1509, 1659
FokI GGATG 4 cut(s) 73, 662, 1375, 1580
FriOI GRGCYC 2 cut(s) 393, 1601
Fsp4HI GCNGC 9 cut(s) 262, 345, 366, 1046, 1232, 1306, 1331, 1509, 1659
FspBI CTAG 7 cut(s) 273, 425, 779, 824, 1130, 1424, 1437
GlaI GCGC 5 cut(s) 448, 1320, 1355, 1370, 1372
GluI GCNGC 9 cut(s) 262, 345, 366, 1046, 1232, 1306, 1331, 1509, 1659
GsaI CCCAGC 2 cut(s) 383, 1377
GsuI CTGGAG 3 cut(s) 146, 1025, 1540
HaeIII GGCC 5 cut(s) 656, 783, 989, 1159, 1717
HapII CCGG 6 cut(s) 307, 331, 691, 853, 1385, 1721
HhaI GCGC 5 cut(s) 449, 1321, 1356, 1371, 1373
Hin1II CATG 9 cut(s) 94, 99, 268, 482, 758, 775, 794, 937, 1183
Hin6I GCGC 5 cut(s) 447, 1319, 1354, 1369, 1371
HinP1I GCGC 5 cut(s) 447, 1319, 1354, 1369, 1371
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 6 cut(s) 121, 151, 212, 878, 1142, 1214
HpaII CCGG 6 cut(s) 307, 331, 691, 853, 1385, 1721
HphI GGTGA 3 cut(s) 887, 896, 1104
Hpy166II GTNNAC 3 cut(s) 270, 683, 887
Hpy188I TCNGA 8 cut(s) 499, 574, 643, 727, 1280, 1596, 1693, 1746
Hpy188III TCNNGA 5 cut(s) 71, 125, 294, 467, 1643
Hpy8I GTNNAC 3 cut(s) 270, 683, 887
HpyAV CCTTC 9 cut(s) 110, 115, 120, 395, 640, 797, 979, 1640, 1741
HpyCH4III ACNGT 2 cut(s) 245, 578
HpyCH4IV ACGT 1 cut(s) 1703
HpyCH4V TGCA 8 cut(s) 22, 661, 926, 1048, 1226, 1508, 1618, 1661
HpyF10VI GCNNNNNNNGC 4 cut(s) 285, 499, 1327, 1664
HpyF3I CTNAG 4 cut(s) 450, 498, 503, 1219
HpySE526I ACGT 1 cut(s) 1703
Hsp92II CATG 9 cut(s) 94, 99, 268, 482, 758, 775, 794, 937, 1183
HspAI GCGC 5 cut(s) 447, 1319, 1354, 1369, 1371
KroI GCCGGC 1 cut(s) 852
KroNI GCCGGC 1 cut(s) 854
Ksp22I TGATCA 1 cut(s) 1345
Kzo9I GATC 4 cut(s) 73, 310, 1345, 1723
LmnI GCTCC 4 cut(s) 937, 1073, 1264, 1327
Lsp1109I GCAGC 8 cut(s) 273, 356, 377, 1032, 1218, 1292, 1520, 1645
LweI GCATC 4 cut(s) 14, 1213, 1605, 1654
MaeI CTAG 7 cut(s) 273, 425, 779, 824, 1130, 1424, 1437
MaeII ACGT 1 cut(s) 1703
MaeIII GTNAC 5 cut(s) 467, 613, 875, 1445, 1603
MalI GATC 4 cut(s) 75, 312, 1347, 1725
MbiI CCGCTC 1 cut(s) 1330
MboI GATC 4 cut(s) 73, 310, 1345, 1723
MfeI CAATTG 1 cut(s) 1452
MflI RGATCY 1 cut(s) 1723
MhlI GDGCHC 2 cut(s) 393, 1601
MluCI AATT 7 cut(s) 133, 229, 297, 302, 1195, 1452, 1768
MlyI GAGTC 4 cut(s) 115, 872, 1136, 1208
MmeI TCCRAC 1 cut(s) 1405
Mph1103I ATGCAT 1 cut(s) 1620
MroNI GCCGGC 1 cut(s) 852
MseI TTAA 4 cut(s) 1257, 1290, 1587, 1700
MslI CAYNNNNRTG 1 cut(s) 1454
MspA1I CMGCKG 1 cut(s) 1045
MspI CCGG 6 cut(s) 307, 331, 691, 853, 1385, 1721
MspR9I CCNGG 4 cut(s) 308, 331, 518, 1721
MunI CAATTG 1 cut(s) 1452
Mva1269I GAATGC 1 cut(s) 1438
MvaI CCWGG 1 cut(s) 518
MvnI CGCG 2 cut(s) 1321, 1371
MwoI GCNNNNNNNGC 4 cut(s) 285, 499, 1327, 1664
NaeI GCCGGC 1 cut(s) 854
NciI CCSGG 3 cut(s) 308, 331, 1721
NdeII GATC 4 cut(s) 73, 310, 1345, 1723
NgoMIV GCCGGC 1 cut(s) 852
NlaIII CATG 9 cut(s) 94, 99, 268, 482, 758, 775, 794, 937, 1183
NlaIV GGNNCC 5 cut(s) 1314, 1379, 1389, 1534, 1718
NmuCI GTSAC 3 cut(s) 467, 875, 1603
NsiI ATGCAT 1 cut(s) 1620
NspI RCATGY 1 cut(s) 268
NspV TTCGAA 1 cut(s) 859
OliI CACNNNNGTG 1 cut(s) 1454
PaeR7I CTCGAG 1 cut(s) 1144
PauI GCGCGC 1 cut(s) 1369
PceI AGGCCT 2 cut(s) 656, 783
PcsI WCGNNNNNNNCGW 1 cut(s) 856
PctI GAATGC 1 cut(s) 1438
PdiI GCCGGC 1 cut(s) 854
PfeI GAWTC 2 cut(s) 151, 212
PfoI TCCNGGA 1 cut(s) 306
PkrI GCNGC 9 cut(s) 263, 346, 367, 1047, 1233, 1307, 1332, 1510, 1660
PleI GAGTC 4 cut(s) 115, 872, 1136, 1208
PpsI GAGTC 4 cut(s) 115, 872, 1136, 1208
PpuMI RGGWCCY 1 cut(s) 1313
PsiI TTATAA 1 cut(s) 221
Psp124BI GAGCTC 1 cut(s) 1601
Psp5II RGGWCCY 1 cut(s) 1313
Psp6I CCWGG 1 cut(s) 516
PspFI CCCAGC 2 cut(s) 379, 1373
PspGI CCWGG 1 cut(s) 516
PspN4I GGNNCC 5 cut(s) 1314, 1379, 1389, 1534, 1718
PspPI GGNCC 3 cut(s) 1157, 1313, 1716
PspPPI RGGWCCY 1 cut(s) 1313
PspXI VCTCGAGB 1 cut(s) 1144
PstI CTGCAG 1 cut(s) 1663
PsuI RGATCY 1 cut(s) 1723
PteI GCGCGC 1 cut(s) 1369
PvuII CAGCTG 1 cut(s) 1045
RsaI GTAC 1 cut(s) 226
RsaNI GTAC 1 cut(s) 225
RseI CAYNNNNRTG 1 cut(s) 1454
SacI GAGCTC 1 cut(s) 1601
SaqAI TTAA 4 cut(s) 1257, 1290, 1587, 1700
SatI GCNGC 9 cut(s) 262, 345, 366, 1046, 1232, 1306, 1331, 1509, 1659
Sau3AI GATC 4 cut(s) 73, 310, 1345, 1723
Sau96I GGNCC 3 cut(s) 1157, 1313, 1716
ScaI AGTACT 1 cut(s) 226
SchI GAGTC 4 cut(s) 115, 872, 1136, 1208
ScrFI CCNGG 4 cut(s) 308, 331, 518, 1721
SduI GDGCHC 2 cut(s) 393, 1601
SfaNI GCATC 4 cut(s) 14, 1213, 1605, 1654
SfcI CTRYAG 1 cut(s) 1659
Sfr274I CTCGAG 1 cut(s) 1144
SfuI TTCGAA 1 cut(s) 859
SinI GGWCC 1 cut(s) 1313
SlaI CTCGAG 1 cut(s) 1144
SmiMI CAYNNNNRTG 1 cut(s) 1454
SmlI CTYRAG 1 cut(s) 1144
SmoI CTYRAG 1 cut(s) 1144
SpeI ACTAGT 2 cut(s) 272, 424
Sse9I AATT 7 cut(s) 133, 229, 297, 302, 1195, 1452, 1768
SseBI AGGCCT 2 cut(s) 656, 783
SsiI CCGC 1 cut(s) 1330
SspI AATATT 1 cut(s) 1591
SspMI CTAG 7 cut(s) 273, 425, 779, 824, 1130, 1424, 1437
SstI GAGCTC 1 cut(s) 1601
StuI AGGCCT 2 cut(s) 656, 783
StyD4I CCNGG 4 cut(s) 306, 329, 516, 1719
StyI CCWWGG 1 cut(s) 778
TaaI ACNGT 2 cut(s) 245, 578
TaiI ACGT 1 cut(s) 1706
TaqI TCGA 5 cut(s) 119, 859, 1145, 1212, 1493
TasI AATT 7 cut(s) 133, 229, 297, 302, 1195, 1452, 1768
TatI WGTACW 1 cut(s) 224
TauI GCSGC 1 cut(s) 1333
TfiI GAWTC 2 cut(s) 151, 212
Tru1I TTAA 4 cut(s) 1257, 1290, 1587, 1700
Tru9I TTAA 4 cut(s) 1257, 1290, 1587, 1700
TscAI CASTG 5 cut(s) 250, 256, 262, 603, 966
TseFI GTSAC 3 cut(s) 467, 875, 1603
TseI GCWGC 8 cut(s) 261, 344, 365, 1045, 1231, 1305, 1508, 1658
Tsp45I GTSAC 3 cut(s) 467, 875, 1603
TspGWI ACGGA 2 cut(s) 144, 1613
TspRI CASTG 5 cut(s) 250, 256, 262, 603, 966
VpaK11BI GGWCC 1 cut(s) 1313
XapI RAATTY 1 cut(s) 297
XceI RCATGY 1 cut(s) 268
XhoI CTCGAG 1 cut(s) 1144
XmaJI CCTAGG 1 cut(s) 778
XmiI GTMKAC 1 cut(s) 269
XspI CTAG 7 cut(s) 273, 425, 779, 824, 1130, 1424, 1437
ZrmI AGTACT 1 cut(s) 226
Zsp2I ATGCAT 1 cut(s) 1620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.