Rmu_ssc0000486.1_g000031

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000486.1
OG0014728
Physical Location & Seq
Reverse (-)
119705 .. 121652
1948 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000486.1_g000031.1.cds

Sequence Viewer

Length: 831 bp
atgtcaataatactcctgccgcagcttgatcatcatcggagcagcgtcggtatacatagcatgccggacgtgccttcgtccttgtccttggaagccatccacgaattcgaagaagactgctacccctactgcgaatctgatgatgaggggagtttgttttctattgatttcaattgtatcaatcacaagacgaagccggatcaaatcattcaggaagaaagggtttatcacgtcgcagtggacctgatgaagagcgagtctagcatggatgcccttctctggacgctcagccaagccgttactcattcatcatccaatatcaaaaccgtgtatcatgtcaaactcgtacatgtctttccaccaattcgattcattccttctccattaggaatgcttccaagaagtaaagtgagtccaaagatggtgaataagtacctggctcaagaaagagacaaaaggaccaagctccttgagaaatacgttgctgcatgcttaaccgccaaggttaaggtcgatgttatatttattgagagtgatacaattgcaaaggccgtcctaaaccttattccaactggaaacgtgaagaatctagttgttggaacaacgatatccaatctcaggaaactgaggtctagaaagggaacaggggttgctaatcagatactacgaaatgcatctccagaaacgacgtcctgctgcgatataaagatcatatgcaagggaaaggaagtggtgattgatcagcttattatcggatcgccttcctcgcgtagcagtaatgcaaattccttgtcaacgcaagaggatcaagatcaagaagtagcaatgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

276

Amino Acids

30.66

Weight (kDa)

6.75

Isoelectric Point (pI)

56.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014728)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g32790 FvH4_4g32790
malus_domestica MD13G1022500.v1.1 MD16G1023800.v1.1
prunus_persica Prupe.1G329100_v2.0.a1
pyrus_communis pycom13g02100
rosa_chinensis RchiOBHm_Chr4g0441781
rosa_laevigata RLG00000006066
rosa_multiflora Rmu_sc0013960.1_g000007 Rmu_ssc0000486.1_g000031
rosa_roxburghii Rroxscaffold_5G00382610
rosa_rugosa Rorug04G0336200
rosa_samantha Rh4AG387500 Rh4BG401300 Rh4CG416300 Rh4DG394500
rosa_wichuraiana Rw4G033450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 692
AccI GTMKAC 1 cut(s) 52
AccII CGCG 1 cut(s) 769
AciI CCGC 2 cut(s) 20, 498
AclWI GGATC 3 cut(s) 207, 763, 813
AcsI RAATTY 2 cut(s) 104, 784
AcyI GRCGYC 1 cut(s) 689
AfaI GTAC 2 cut(s) 348, 434
AfiI CCNNNNNNNGG 2 cut(s) 279, 618
AflIII ACRYGT 1 cut(s) 349
AgsI TTSAA 1 cut(s) 172
AjiI CACGTC 2 cut(s) 70, 232
AjnI CCWGG 1 cut(s) 435
AluBI AGCT 3 cut(s) 25, 466, 745
AluI AGCT 3 cut(s) 25, 466, 745
Alw26I GTCTC 1 cut(s) 444
AlwI GGATC 3 cut(s) 207, 763, 813
AoxI GGCC 1 cut(s) 549
ApeKI GCWGC 4 cut(s) 22, 42, 485, 696
ApoI RAATTY 2 cut(s) 104, 784
ArsI GACNNNNNNTTYG 2 cut(s) 776, 808
AspS9I GGNCC 2 cut(s) 241, 459
AsuHPI GGTGA 2 cut(s) 436, 745
AsuII TTCGAA 1 cut(s) 108
AvaII GGWCC 2 cut(s) 241, 459
BbsI GAAGAC 1 cut(s) 120
BbvI GCAGC 4 cut(s) 34, 54, 472, 683
BccI CCATC 2 cut(s) 104, 415
BceAI ACGGC 2 cut(s) 281, 536
BciT130I CCWGG 1 cut(s) 437
BclI TGATCA 2 cut(s) 28, 739
BcoDI GTCTC 1 cut(s) 444
BfaI CTAG 3 cut(s) 261, 590, 633
BisI GCNGC 5 cut(s) 20, 23, 43, 486, 697
BlpI GCTNAGC 1 cut(s) 287
BlsI GCNGC 5 cut(s) 21, 24, 44, 487, 698
Bme1390I CCNGG 1 cut(s) 437
Bme18I GGWCC 2 cut(s) 241, 459
BmgBI CACGTC 2 cut(s) 70, 232
BmgT120I GGNCC 2 cut(s) 241, 459
BmrFI CCNGG 1 cut(s) 437
BmsI GCATC 2 cut(s) 259, 683
BpiI GAAGAC 1 cut(s) 120
BpmI CTGGAG 1 cut(s) 663
Bpu1102I GCTNAGC 1 cut(s) 287
Bpu14I TTCGAA 1 cut(s) 108
BpuEI CTTGAG 2 cut(s) 426, 491
BsaBI GATNNNNATC 2 cut(s) 33, 810
BsaHI GRCGYC 1 cut(s) 689
BsaJI CCNNGG 2 cut(s) 87, 501
Bsc4I CCNNNNNNNGG 2 cut(s) 279, 618
Bse1I ACTGG 1 cut(s) 577
Bse3DI GCAATG 1 cut(s) 831
Bse8I GATNNNNATC 2 cut(s) 33, 810
BseBI CCWGG 1 cut(s) 437
BseDI CCNNGG 2 cut(s) 87, 501
BseGI GGATG 3 cut(s) 96, 274, 311
BseJI GATNNNNATC 2 cut(s) 33, 810
BseLI CCNNNNNNNGG 2 cut(s) 279, 618
BseMI GCAATG 1 cut(s) 831
BseMII CTCAG 3 cut(s) 301, 617, 631
BseNI ACTGG 1 cut(s) 577
BseXI GCAGC 4 cut(s) 34, 54, 472, 683
Bsh1236I CGCG 1 cut(s) 769
BshFI GGCC 1 cut(s) 551
BsiSI CCGG 2 cut(s) 65, 197
BslI CCNNNNNNNGG 2 cut(s) 279, 618
BsmAI GTCTC 1 cut(s) 444
BsmI GAATGC 1 cut(s) 396
BsnI GGCC 1 cut(s) 551
Bsp119I TTCGAA 1 cut(s) 108
Bsp143I GATC 7 cut(s) 28, 199, 708, 739, 755, 805, 811
Bsp1720I GCTNAGC 1 cut(s) 287
BspACI CCGC 2 cut(s) 20, 498
BspANI GGCC 1 cut(s) 551
BspCNI CTCAG 3 cut(s) 300, 618, 630
BspFNI CGCG 1 cut(s) 769
BspPI GGATC 3 cut(s) 207, 763, 813
BspQI GCTCTTC 1 cut(s) 245
BspT104I TTCGAA 1 cut(s) 108
BsrDI GCAATG 1 cut(s) 831
BsrI ACTGG 1 cut(s) 577
BssECI CCNNGG 2 cut(s) 87, 501
BssMI GATC 7 cut(s) 28, 199, 708, 739, 755, 805, 811
BssNAI GTATAC 1 cut(s) 53
BssNI GRCGYC 1 cut(s) 689
BssT1I CCWWGG 2 cut(s) 87, 501
Bst1107I GTATAC 1 cut(s) 53
Bst2UI CCWGG 1 cut(s) 437
Bst4CI ACNGT 1 cut(s) 328
Bst6I CTCTTC 1 cut(s) 245
BstACI GRCGYC 1 cut(s) 689
BstBI TTCGAA 1 cut(s) 108
BstC8I GCNNGC 2 cut(s) 62, 490
BstDEI CTNAG 3 cut(s) 287, 617, 626
BstF5I GGATG 3 cut(s) 96, 274, 311
BstFNI CGCG 1 cut(s) 769
BstKTI GATC 7 cut(s) 31, 202, 711, 742, 758, 808, 814
BstMAI GTCTC 1 cut(s) 444
BstMBI GATC 7 cut(s) 28, 199, 708, 739, 755, 805, 811
BstMWI GCNNNNNNNGC 3 cut(s) 70, 261, 766
BstNI CCWGG 1 cut(s) 437
BstNSI RCATGY 3 cut(s) 64, 353, 492
BstSCI CCNGG 1 cut(s) 435
BstUI CGCG 1 cut(s) 769
BstV1I GCAGC 4 cut(s) 34, 54, 472, 683
BstV2I GAAGAC 1 cut(s) 120
BstZ17I GTATAC 1 cut(s) 53
BsuRI GGCC 1 cut(s) 551
BtrI CACGTC 2 cut(s) 70, 232
BtsCI GGATG 3 cut(s) 96, 274, 311
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 2 cut(s) 62, 490
Cfr13I GGNCC 2 cut(s) 241, 459
CseI GACGC 2 cut(s) 34, 292
Csp6I GTAC 2 cut(s) 347, 433
CviAII CATG 5 cut(s) 61, 265, 335, 350, 489
CviJI RGCY 9 cut(s) 25, 95, 196, 291, 296, 440, 466, 551, 745
CviKI_1 RGCY 9 cut(s) 25, 95, 196, 291, 296, 440, 466, 551, 745
CviQI GTAC 2 cut(s) 347, 433
DdeI CTNAG 3 cut(s) 287, 617, 626
DpnI GATC 7 cut(s) 30, 201, 710, 741, 757, 807, 813
DpnII GATC 7 cut(s) 28, 199, 708, 739, 755, 805, 811
Eam1104I CTCTTC 1 cut(s) 245
EarI CTCTTC 1 cut(s) 245
Eco130I CCWWGG 2 cut(s) 87, 501
Eco32I GATATC 1 cut(s) 609
Eco47I GGWCC 2 cut(s) 241, 459
EcoRI GAATTC 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 435
EcoRV GATATC 1 cut(s) 609
EcoT14I CCWWGG 2 cut(s) 87, 501
EcoT22I ATGCAT 1 cut(s) 676
ErhI CCWWGG 2 cut(s) 87, 501
FaeI CATG 5 cut(s) 64, 268, 338, 353, 492
FatI CATG 5 cut(s) 60, 264, 334, 349, 488
FauNDI CATATG 1 cut(s) 713
FbaI TGATCA 2 cut(s) 28, 739
FblI GTMKAC 1 cut(s) 52
Fnu4HI GCNGC 5 cut(s) 20, 23, 43, 486, 697
FokI GGATG 3 cut(s) 83, 281, 298
Fsp4HI GCNGC 5 cut(s) 20, 23, 43, 486, 697
FspBI CTAG 3 cut(s) 261, 590, 633
GluI GCNGC 5 cut(s) 20, 23, 43, 486, 697
GsuI CTGGAG 1 cut(s) 663
HaeIII GGCC 1 cut(s) 551
HapII CCGG 2 cut(s) 65, 197
HgaI GACGC 2 cut(s) 34, 292
Hin1I GRCGYC 1 cut(s) 689
Hin1II CATG 5 cut(s) 64, 268, 338, 353, 492
HincII GTYRAC 1 cut(s) 795
HindII GTYRAC 1 cut(s) 795
HinfI GANTC 5 cut(s) 134, 257, 369, 412, 586
HpaII CCGG 2 cut(s) 65, 197
HphI GGTGA 2 cut(s) 436, 745
Hpy166II GTNNAC 3 cut(s) 53, 241, 795
Hpy188I TCNGA 4 cut(s) 39, 139, 660, 755
Hpy188III TCNNGA 8 cut(s) 212, 280, 443, 619, 633, 680, 809, 815
Hpy8I GTNNAC 3 cut(s) 53, 241, 795
Hpy99I CGWCG 3 cut(s) 50, 236, 691
HpyAV CCTTC 4 cut(s) 84, 284, 387, 771
HpyCH4III ACNGT 1 cut(s) 328
HpyCH4IV ACGT 5 cut(s) 69, 231, 480, 579, 689
HpyCH4V TGCA 5 cut(s) 488, 545, 674, 717, 782
HpyF10VI GCNNNNNNNGC 3 cut(s) 70, 261, 766
HpyF3I CTNAG 3 cut(s) 287, 617, 626
HpySE526I ACGT 5 cut(s) 69, 231, 480, 579, 689
Hsp92I GRCGYC 1 cut(s) 689
Hsp92II CATG 5 cut(s) 64, 268, 338, 353, 492
Ksp22I TGATCA 2 cut(s) 28, 739
Kzo9I GATC 7 cut(s) 28, 199, 708, 739, 755, 805, 811
LguI GCTCTTC 1 cut(s) 245
LmnI GCTCC 2 cut(s) 39, 471
Lsp1109I GCAGC 4 cut(s) 34, 54, 472, 683
LweI GCATC 2 cut(s) 259, 683
MaeI CTAG 3 cut(s) 261, 590, 633
MaeII ACGT 5 cut(s) 69, 231, 480, 579, 689
MaeIII GTNAC 1 cut(s) 298
MalI GATC 7 cut(s) 30, 201, 710, 741, 757, 807, 813
MboI GATC 7 cut(s) 28, 199, 708, 739, 755, 805, 811
MboII GAAGA 5 cut(s) 122, 125, 227, 262, 595
MfeI CAATTG 2 cut(s) 172, 540
MluCI AATT 5 cut(s) 104, 172, 363, 540, 784
MlyI GAGTC 2 cut(s) 266, 421
MmeI TCCRAC 2 cut(s) 577, 593
MnlI CCTC 4 cut(s) 139, 621, 775, 796
Mph1103I ATGCAT 1 cut(s) 676
MseI TTAA 2 cut(s) 494, 507
MspI CCGG 2 cut(s) 65, 197
MspR9I CCNGG 1 cut(s) 437
MunI CAATTG 2 cut(s) 172, 540
Mva1269I GAATGC 1 cut(s) 396
MvaI CCWGG 1 cut(s) 437
MvnI CGCG 1 cut(s) 769
MwoI GCNNNNNNNGC 3 cut(s) 70, 261, 766
NdeI CATATG 1 cut(s) 713
NdeII GATC 7 cut(s) 28, 199, 708, 739, 755, 805, 811
NlaIII CATG 5 cut(s) 64, 268, 338, 353, 492
NsiI ATGCAT 1 cut(s) 676
NspI RCATGY 3 cut(s) 64, 353, 492
NspV TTCGAA 1 cut(s) 108
PaeI GCATGC 2 cut(s) 64, 492
PciI ACATGT 1 cut(s) 349
PciSI GCTCTTC 1 cut(s) 245
PctI GAATGC 1 cut(s) 396
PfeI GAWTC 3 cut(s) 134, 369, 586
PkrI GCNGC 5 cut(s) 21, 24, 44, 487, 698
PleI GAGTC 2 cut(s) 265, 420
PpsI GAGTC 2 cut(s) 265, 420
PscI ACATGT 1 cut(s) 349
Psp6I CCWGG 1 cut(s) 435
PspGI CCWGG 1 cut(s) 435
PspPI GGNCC 2 cut(s) 241, 459
RsaI GTAC 2 cut(s) 348, 434
RsaNI GTAC 2 cut(s) 347, 433
SapI GCTCTTC 1 cut(s) 245
SaqAI TTAA 2 cut(s) 494, 507
SatI GCNGC 5 cut(s) 20, 23, 43, 486, 697
Sau3AI GATC 7 cut(s) 28, 199, 708, 739, 755, 805, 811
Sau96I GGNCC 2 cut(s) 241, 459
SchI GAGTC 2 cut(s) 266, 421
ScrFI CCNGG 1 cut(s) 437
SfaNI GCATC 2 cut(s) 259, 683
SfuI TTCGAA 1 cut(s) 108
SinI GGWCC 2 cut(s) 241, 459
SmlI CTYRAG 2 cut(s) 441, 470
SmoI CTYRAG 2 cut(s) 441, 470
SphI GCATGC 2 cut(s) 64, 492
Sse9I AATT 5 cut(s) 104, 172, 363, 540, 784
SsiI CCGC 2 cut(s) 20, 498
SspMI CTAG 3 cut(s) 261, 590, 633
StyD4I CCNGG 1 cut(s) 435
StyI CCWWGG 2 cut(s) 87, 501
TaaI ACNGT 1 cut(s) 328
TaiI ACGT 5 cut(s) 72, 234, 483, 582, 692
TaqI TCGA 3 cut(s) 108, 367, 513
TasI AATT 5 cut(s) 104, 172, 363, 540, 784
TauI GCSGC 1 cut(s) 22
TfiI GAWTC 3 cut(s) 134, 369, 586
Tru1I TTAA 2 cut(s) 494, 507
Tru9I TTAA 2 cut(s) 494, 507
TscAI CASTG 1 cut(s) 243
TseI GCWGC 4 cut(s) 22, 42, 485, 696
TspDTI ATGAA 3 cut(s) 263, 297, 361
TspRI CASTG 1 cut(s) 243
VpaK11BI GGWCC 2 cut(s) 241, 459
XapI RAATTY 2 cut(s) 104, 784
XbaI TCTAGA 1 cut(s) 632
XceI RCATGY 3 cut(s) 64, 353, 492
XmiI GTMKAC 1 cut(s) 52
XspI CTAG 3 cut(s) 261, 590, 633
ZraI GACGTC 1 cut(s) 690
Zsp2I ATGCAT 1 cut(s) 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.