FvH4_4g33570

Protein VAC14 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012295
Physical Location & Seq
Forward (+)
31770977 .. 31777931
6955 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33570.t1

Sequence Viewer

Length: 2181 bp
ATGGCCGACGCTCTCACTGTGATTCCGGCGGGCGTGCTCCGGAACCTGGCGGACAAGCTCTACGAGAAGCGCAAGAATGCTGCTCTCGAGATTGAGGGAATCGTGAAGCAGCTCACGGTGGCCGGCGATCATGATAAGATTACGGCGGTGATCAATTTGCTGACTACGGAGTTTACTTACTCACCGCAGGCGAATCACCGCAAGGGAGGATTGATAGGGTTAGCTGCTGCAACAGTTGGGTTGACCAGTGAAGCTGCTCAGCATCTTGAGCAAATCGTGCCTCCTGTGCTTAATTCCTTTTCTGACCAAGATAGCAGAGTTCGTTATTATGCTTGTGAAGCACTATACAATATTGCAAAGGTTGTACGAGGAGATTTTATCATATTCTTCAATCAGATTTTTGATGCCTTGTGCAAGCTTTCAGCAGACTCAGATGCTAATGTTCAAAGTGCTGCTCATCTTTTAGATCGGCTTGTAAAGGATATTGTTACTGAAAGCGACCAGTTCAGCATTGAAGAATTTATACCATTACTGAGGGAGCGCATGAATGTTCTAAATCCCTATGTCCGGCAGTTTTTGGTAGGGTGGATCACTGTTTTAGACAGTGTTCCAGATATTGATATGCTGGGTTTCCTTCCCGACTTTCTTGATGGCTTGTTTAATATGTTAAGTGATTCAAGCCATGAGATAAGACAACAGGCTGATTCAGCTCTCTCAGAGTTTCTCCAAGAGATCAAGAACTCCCCATCTGTAGATTATGGACGCATGGCTGAAATACTGGTGCAGAGGGCTGCTTCTCCTGATGAATTTACACGTTTAACAGCTATCACATGGATAAATGAGTTTGTAAAACTTGGTGGGGACCAGCTTGTTCCCTACTATGCTGATATATTGGGAGCAATTTTGCCCTGCATATCCGATAAAGAAGAGAAAATCAGAGTGGTTGCTCGCGAAACCAATGAAGAGCTCCGTGCCATTAAGGCAGATCCAGCTGAAGGGTTTGATGTTGGAGCAATCCTCTCTATTGCAAGGAGGCAACTATCTAGTGAATGGGAGGCTACTAGAATCGAAGCATTGCACTGGATTTCCAACCTTCTAAACAGACATCGTCCTGAGGTGTTGACGTATCTGAATGACATATTTGACACCCTTTTGGAAGCACTTTCAGATTCTTCTGATCAGGTGGTTCTCCTGGTTCTAGAGGTTCATGCATGCATAGCGAAAGATCCACAACACTTCCGCCAGCTTGTCGTCTTTCTTGTTCATAATTTCCGTGTAGATAATTCTCTTCTGGAGAAGCGTGGTGCTTTGATAATTCGCCGACTTTGTGTGCTTTTAGATGCTGAAAGGGTTTACCGAGAACTGTCTACTATATTAGAAGGAGAGTCAGACCTGGATTTTGCATCTATTATGGTTCAGGCTTTGAACTTGATTCTACTTACATCTTCCGAGTTGTCTGGCCTTCGAGATCTTTTGAAACAATCACTTGTGAATCCATCAGGGAAGGACTTATTTGTTTCCTTATATGCTTCATGGTGTCACTCACCTATGGCAATTATAAGCCTCTGCTTGTTATCTCAGACATATCAGCATGCGAGTACTGTGATTCAGTCTCTGGTGGAGGAAGATATAAATGTCAAATTTTTAGTCCAGCTGGACAAATTGATTCGGTTACTGGAAACTCCAATCTTTGCATATCTTAGATTACAGCTTCTGGAACCTGGAAGGCATATGTGGCTATTAAAAGCTTTGTATGGTCTTCTGATGCTACTTCCTCAGCAAAGTGCCGCCTTTAGAATTCTACGGACTCGTTTAAAAACTGTACCTACGTACTCATTTGGTGGTGAGCAACCCAGGCGAACTTCGTCAGGGAATCCCTATCAGATCCTACAACACATGTCAGGTGGATCACAGGTCACTGAGGATGGCGACATAAACCCAAACCAGGACAGCAAGTTTTCCCATCATGGAATAAACTTCACTGCAAGACTGCAACAGTTTGAGCAAATGCAGCAGCAGCATCGCCAGCATTCAAAAGTCCAGGCACAACTACGCAGCAATTCCACATCTTCCCCAACGCAACAGGATGTGCAAAGACCTGAAGAACTCAGCCAAACTTCATCAGATGTGAATAGGCCTCCTTCTAGATCTAGGAAAGGCCCGGGGCAGTTACAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05166 ko05203 map05166 map05203
Pfam Domains
Protein Families
Vac14_Fab1_bd Vac14_Fig4_bd

Protein Analysis

727

Amino Acids

81.65

Weight (kDa)

5.48

Isoelectric Point (pI)

48.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOR1L1_N PF24714 15 - 240 9.8e-07 TORTIFOLIA1 N-terminal
Vac14_Fab1_bd PF12755 65 - 161 8e-41 Vacuolar 14 Fab1-binding region
HEAT_EF3_N PF24987 250 - 395 7e-08 EF3 N-terminal HEAT repeat
Vac14_Fig4_bd PF11916 429 - 608 3.7e-72 Vacuolar protein 14 C-terminal Fig4p binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012295)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01690 AT2G01690
fragaria_vesca FvH4_4g33570 FvH4_4g33570
malus_domestica MD13G1020500.v1.1 MD16G1018900.v1.1
prunus_persica Prupe.1G333200_v2.0.a1
pyrus_communis pycom13g01730 pycom16g01700
rosa_chinensis RchiOBHm_Chr4g0443071
rosa_laevigata RLG00000005956
rosa_multiflora Rmu_sc0008069.1_g000004
rosa_roxburghii Rroxscaffold_5G00383700
rosa_rugosa Rorug04G0344200 Rorug04G0344200
rosa_samantha Rh4BG410000 Rh4CG425500 Rh4DG404900
rosa_wichuraiana Rw4G034340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1559, 2179
AccI GTMKAC 1 cut(s) 1367
AccII CGCG 1 cut(s) 951
AccIII TCCGGA 1 cut(s) 39
AciI CCGC 7 cut(s) 29, 50, 146, 185, 199, 1240, 1788
AclWI GGATC 5 cut(s) 596, 980, 1220, 1879, 1915
AcoI YGGCCR 2 cut(s) 3, 120
AcsI RAATTY 4 cut(s) 518, 806, 1640, 1797
AcuI CTGAAG 2 cut(s) 1014, 2121
AfaI GTAC 4 cut(s) 366, 1600, 1824, 1832
AfiI CCNNNNNNNGG 4 cut(s) 46, 567, 995, 1945
AflIII ACRYGT 2 cut(s) 812, 1896
AgsI TTSAA 7 cut(s) 391, 446, 515, 678, 1426, 1477, 2034
AjnI CCWGG 7 cut(s) 45, 1191, 1392, 1720, 1853, 1944, 2040
Alw21I GWGCWC 2 cut(s) 39, 969
Alw26I GTCTC 1 cut(s) 1617
AlwI GGATC 5 cut(s) 596, 980, 1220, 1879, 1915
AlwNI CAGNNNCTG 2 cut(s) 1615, 1714
Ama87I CYCGRG 2 cut(s) 86, 2161
Aor13HI TCCGGA 1 cut(s) 39
AoxI GGCC 5 cut(s) 3, 120, 1459, 2135, 2158
ApoI RAATTY 4 cut(s) 518, 806, 1640, 1797
ArsI GACNNNNNNTTYG 4 cut(s) 1382, 1414, 1632, 1664
AspLEI GCGC 2 cut(s) 72, 543
AspS9I GGNCC 2 cut(s) 862, 2159
AsuC2I CCSGG 2 cut(s) 2162, 2163
AsuHPI GGTGA 5 cut(s) 160, 174, 188, 1536, 1856
AvaI CYCGRG 2 cut(s) 86, 2161
AvaII GGWCC 1 cut(s) 862
AxyI CCTNAGG 1 cut(s) 1113
BanII GRGCYC 1 cut(s) 969
BarI GAAGNNNNNNTAC 2 cut(s) 507, 539
BbsI GAAGAC 1 cut(s) 1751
Bbv12I GWGCWC 2 cut(s) 39, 969
BbvCI CCTCAGC 1 cut(s) 1776
BccI CCATC 5 cut(s) 644, 754, 1504, 1919, 1971
BceAI ACGGC 1 cut(s) 159
BcgI CGANNNNNNTGC 2 cut(s) 2003, 2037
BciT130I CCWGG 7 cut(s) 47, 1193, 1394, 1722, 1855, 1946, 2042
BclI TGATCA 2 cut(s) 150, 1177
BcnI CCSGG 2 cut(s) 2162, 2163
BcoDI GTCTC 1 cut(s) 1617
BfaI CTAG 5 cut(s) 1044, 1062, 1199, 2145, 2151
BfmI CTRYAG 1 cut(s) 750
BglI GCCNNNNNGGC 1 cut(s) 980
BglII AGATCT 2 cut(s) 1468, 2147
BlpI GCTNAGC 1 cut(s) 258
BmcAI AGTACT 1 cut(s) 1600
Bme1390I CCNGG 9 cut(s) 47, 1193, 1394, 1722, 1855, 1946, 2042, 2162, 2163
Bme18I GGWCC 1 cut(s) 862
BmeT110I CYCGRG 2 cut(s) 86, 2161
BmgT120I GGNCC 2 cut(s) 862, 2159
BmiI GGNNCC 3 cut(s) 44, 863, 1719
BmrFI CCNGG 9 cut(s) 47, 1193, 1394, 1722, 1855, 1946, 2042, 2162, 2163
BmsI GCATC 7 cut(s) 271, 394, 424, 1330, 1412, 1755, 2029
BpiI GAAGAC 1 cut(s) 1751
BplI GAGNNNNNCTC 2 cut(s) 1002, 1034
BpmI CTGGAG 1 cut(s) 1313
Bpu10I CCTNAGC 1 cut(s) 1776
Bpu1102I GCTNAGC 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 2 cut(s) 2162, 2163
BsaAI YACGTR 1 cut(s) 1830
BsaJI CCNNGG 3 cut(s) 1853, 2161, 2162
BsaWI WCCGGW 1 cut(s) 39
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 567, 995, 1945
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 5 cut(s) 246, 502, 783, 1085, 1680
Bse21I CCTNAGG 1 cut(s) 1113
Bse3DI GCAATG 1 cut(s) 1073
BseAI TCCGGA 1 cut(s) 39
BseBI CCWGG 7 cut(s) 47, 1193, 1394, 1722, 1855, 1946, 2042
BseDI CCNNGG 3 cut(s) 1853, 2161, 2162
BseGI GGATG 2 cut(s) 1930, 2092
BseLI CCNNNNNNNGG 4 cut(s) 46, 567, 995, 1945
BseMI GCAATG 1 cut(s) 1073
BseMII CTCAG 9 cut(s) 272, 444, 524, 729, 1104, 1592, 1790, 1911, 2122
BseNI ACTGG 5 cut(s) 246, 502, 783, 1085, 1680
BseRI GAGGAG 1 cut(s) 384
BseYI CCCAGC 1 cut(s) 625
BsgI GTGCAG 1 cut(s) 803
Bsh1236I CGCG 1 cut(s) 951
BshFI GGCC 5 cut(s) 5, 122, 1461, 2137, 2160
BsiHKAI GWGCWC 2 cut(s) 39, 969
BsiHKCI CYCGRG 2 cut(s) 86, 2161
BsiSI CCGG 5 cut(s) 26, 40, 123, 568, 2162
BslFI GGGAC 1 cut(s) 875
BslI CCNNNNNNNGG 4 cut(s) 46, 567, 995, 1945
BsmAI GTCTC 1 cut(s) 1617
BsmFI GGGAC 1 cut(s) 875
BsmI GAATGC 2 cut(s) 82, 2029
BsnI GGCC 5 cut(s) 5, 122, 1461, 2137, 2160
BsoBI CYCGRG 2 cut(s) 86, 2161
Bsp1286I GDGCHC 2 cut(s) 39, 969
Bsp13I TCCGGA 1 cut(s) 39
Bsp1720I GCTNAGC 1 cut(s) 258
Bsp68I TCGCGA 1 cut(s) 951
BspACI CCGC 7 cut(s) 29, 50, 146, 185, 199, 1240, 1788
BspANI GGCC 5 cut(s) 5, 122, 1461, 2137, 2160
BspCNI CTCAG 9 cut(s) 271, 443, 525, 728, 1105, 1591, 1789, 1912, 2121
BspEI TCCGGA 1 cut(s) 39
BspFNI CGCG 1 cut(s) 951
BspHI TCATGA 1 cut(s) 130
BspLI GGNNCC 3 cut(s) 44, 863, 1719
BspPI GGATC 5 cut(s) 596, 980, 1220, 1879, 1915
BspQI GCTCTTC 1 cut(s) 957
BsrDI GCAATG 1 cut(s) 1073
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 5 cut(s) 246, 502, 783, 1085, 1680
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 3 cut(s) 1853, 2161, 2162
Bst2UI CCWGG 7 cut(s) 47, 1193, 1394, 1722, 1855, 1946, 2042
Bst4CI ACNGT 9 cut(s) 19, 118, 235, 595, 605, 1365, 1603, 1822, 1998
Bst6I CTCTTC 3 cut(s) 921, 957, 1293
BstAPI GCANNNNNTGC 1 cut(s) 277
BstBAI YACGTR 1 cut(s) 1830
BstF5I GGATG 2 cut(s) 1930, 2092
BstFNI CGCG 1 cut(s) 951
BstHHI GCGC 2 cut(s) 72, 543
BstMAI GTCTC 1 cut(s) 1617
BstNI CCWGG 7 cut(s) 47, 1193, 1394, 1722, 1855, 1946, 2042
BstNSI RCATGY 3 cut(s) 1215, 1595, 1900
BstSCI CCNGG 9 cut(s) 45, 1191, 1392, 1720, 1853, 1944, 2040, 2160, 2161
BstSFI CTRYAG 1 cut(s) 750
BstSNI TACGTA 1 cut(s) 1830
BstUI CGCG 1 cut(s) 951
BstV2I GAAGAC 1 cut(s) 1751
BstX2I RGATCY 5 cut(s) 985, 1225, 1468, 1884, 2147
BstYI RGATCY 5 cut(s) 985, 1225, 1468, 1884, 2147
Bsu36I CCTNAGG 1 cut(s) 1113
BsuRI GGCC 5 cut(s) 5, 122, 1461, 2137, 2160
BtgZI GCGATG 1 cut(s) 2006
BtsCI GGATG 2 cut(s) 1930, 2092
BtsI GCAGTG 1 cut(s) 1980
BtsIMutI CAGTG 7 cut(s) 15, 253, 591, 610, 1078, 1917, 1980
BtuMI TCGCGA 1 cut(s) 951
CaiI CAGNNNCTG 2 cut(s) 1615, 1714
CciI TCATGA 1 cut(s) 130
CfoI GCGC 2 cut(s) 72, 543
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 2 cut(s) 862, 2159
Cfr9I CCCGGG 1 cut(s) 2161
CseI GACGC 2 cut(s) 17, 771
Csp6I GTAC 4 cut(s) 365, 1599, 1823, 1831
CviQI GTAC 4 cut(s) 365, 1599, 1823, 1831
DraI TTTAAA 1 cut(s) 1815
EaeI YGGCCR 2 cut(s) 3, 120
Eam1104I CTCTTC 3 cut(s) 921, 957, 1293
EarI CTCTTC 3 cut(s) 921, 957, 1293
EciI GGCGGA 2 cut(s) 65, 1229
Ecl136II GAGCTC 1 cut(s) 967
Eco105I TACGTA 1 cut(s) 1830
Eco147I AGGCCT 1 cut(s) 2137
Eco24I GRGCYC 1 cut(s) 969
Eco47I GGWCC 1 cut(s) 862
Eco53kI GAGCTC 1 cut(s) 967
Eco57I CTGAAG 2 cut(s) 1014, 2121
Eco81I CCTNAGG 1 cut(s) 1113
Eco88I CYCGRG 2 cut(s) 86, 2161
EcoICRI GAGCTC 1 cut(s) 967
EcoRI GAATTC 1 cut(s) 1797
EcoRII CCWGG 7 cut(s) 45, 1191, 1392, 1720, 1853, 1944, 2040
EcoT22I ATGCAT 2 cut(s) 1213, 1217
EcoT38I GRGCYC 1 cut(s) 969
FaqI GGGAC 1 cut(s) 875
FauI CCCGC 1 cut(s) 22
FauNDI CATATG 1 cut(s) 1731
FbaI TGATCA 2 cut(s) 150, 1177
FblI GTMKAC 1 cut(s) 1367
FokI GGATG 2 cut(s) 1937, 2099
FriOI GRGCYC 1 cut(s) 969
FspBI CTAG 5 cut(s) 1044, 1062, 1199, 2145, 2151
GlaI GCGC 2 cut(s) 71, 542
GsaI CCCAGC 1 cut(s) 629
GsuI CTGGAG 1 cut(s) 1313
HaeIII GGCC 5 cut(s) 5, 122, 1461, 2137, 2160
HapII CCGG 5 cut(s) 26, 40, 123, 568, 2162
HgaI GACGC 2 cut(s) 17, 771
HhaI GCGC 2 cut(s) 72, 543
Hin6I GCGC 2 cut(s) 70, 541
HinP1I GCGC 2 cut(s) 70, 541
HincII GTYRAC 2 cut(s) 243, 1122
HindII GTYRAC 2 cut(s) 243, 1122
HindIII AAGCTT 2 cut(s) 416, 1746
HpaII CCGG 5 cut(s) 26, 40, 123, 568, 2162
HphI GGTGA 5 cut(s) 160, 174, 188, 1536, 1856
Hpy166II GTNNAC 5 cut(s) 174, 243, 1122, 1354, 1368
Hpy8I GTNNAC 5 cut(s) 174, 243, 1122, 1354, 1368
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 8 cut(s) 644, 989, 1103, 1373, 1472, 1498, 1719, 2151
HpyCH4III ACNGT 9 cut(s) 19, 118, 235, 595, 605, 1365, 1603, 1822, 1998
HpyCH4IV ACGT 3 cut(s) 814, 1124, 1829
HpySE526I ACGT 3 cut(s) 814, 1124, 1829
HspAI GCGC 2 cut(s) 70, 541
Kpn2I TCCGGA 1 cut(s) 39
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
Ksp22I TGATCA 2 cut(s) 150, 1177
LguI GCTCTTC 1 cut(s) 957
LmnI GCTCC 5 cut(s) 42, 538, 896, 972, 1010
LweI GCATC 7 cut(s) 271, 394, 424, 1330, 1412, 1755, 2029
MaeI CTAG 5 cut(s) 1044, 1062, 1199, 2145, 2151
MaeII ACGT 3 cut(s) 814, 1124, 1829
MaeIII GTNAC 5 cut(s) 487, 1538, 1671, 1915, 2169
MflI RGATCY 5 cut(s) 985, 1225, 1468, 1884, 2147
MhlI GDGCHC 2 cut(s) 39, 969
MlyI GAGTC 3 cut(s) 422, 1394, 1801
MmeI TCCRAC 2 cut(s) 988, 1113
Mph1103I ATGCAT 2 cut(s) 1213, 1217
MroI TCCGGA 1 cut(s) 39
MroNI GCCGGC 1 cut(s) 122
MseI TTAA 7 cut(s) 291, 660, 668, 818, 978, 1742, 1814
MspA1I CMGCKG 2 cut(s) 992, 1654
MspI CCGG 5 cut(s) 26, 40, 123, 568, 2162
MspR9I CCNGG 9 cut(s) 47, 1193, 1394, 1722, 1855, 1946, 2042, 2162, 2163
Mva1269I GAATGC 2 cut(s) 82, 2029
MvaI CCWGG 7 cut(s) 47, 1193, 1394, 1722, 1855, 1946, 2042
MvnI CGCG 1 cut(s) 951
NaeI GCCGGC 1 cut(s) 124
NciI CCSGG 2 cut(s) 2162, 2163
NdeI CATATG 1 cut(s) 1731
NgoMIV GCCGGC 1 cut(s) 122
NlaIV GGNNCC 3 cut(s) 44, 863, 1719
NmuCI GTSAC 2 cut(s) 1538, 1915
NruI TCGCGA 1 cut(s) 951
NsiI ATGCAT 2 cut(s) 1213, 1217
NspI RCATGY 3 cut(s) 1215, 1595, 1900
PaeI GCATGC 2 cut(s) 1215, 1595
PaeR7I CTCGAG 1 cut(s) 86
PagI TCATGA 1 cut(s) 130
PceI AGGCCT 1 cut(s) 2137
PciI ACATGT 1 cut(s) 1896
PciSI GCTCTTC 1 cut(s) 957
PctI GAATGC 2 cut(s) 82, 2029
PdiI GCCGGC 1 cut(s) 124
PflFI GACNNNGTC 1 cut(s) 1107
PleI GAGTC 3 cut(s) 422, 1393, 1801
PpsI GAGTC 3 cut(s) 422, 1393, 1801
Ppu21I YACGTR 1 cut(s) 1830
PscI ACATGT 1 cut(s) 1896
PsiI TTATAA 2 cut(s) 1559, 2179
Psp124BI GAGCTC 1 cut(s) 969
Psp6I CCWGG 7 cut(s) 45, 1191, 1392, 1720, 1853, 1944, 2040
PspFI CCCAGC 1 cut(s) 625
PspGI CCWGG 7 cut(s) 45, 1191, 1392, 1720, 1853, 1944, 2040
PspN4I GGNNCC 3 cut(s) 44, 863, 1719
PspPI GGNCC 2 cut(s) 862, 2159
PstNI CAGNNNCTG 2 cut(s) 1615, 1714
PsuI RGATCY 5 cut(s) 985, 1225, 1468, 1884, 2147
PsyI GACNNNGTC 1 cut(s) 1107
PvuII CAGCTG 2 cut(s) 992, 1654
RruI TCGCGA 1 cut(s) 951
RsaI GTAC 4 cut(s) 366, 1600, 1824, 1832
RsaNI GTAC 4 cut(s) 365, 1599, 1823, 1831
SacI GAGCTC 1 cut(s) 969
SapI GCTCTTC 1 cut(s) 957
SaqAI TTAA 7 cut(s) 291, 660, 668, 818, 978, 1742, 1814
Sau96I GGNCC 2 cut(s) 862, 2159
ScaI AGTACT 1 cut(s) 1600
SchI GAGTC 3 cut(s) 422, 1394, 1801
ScrFI CCNGG 9 cut(s) 47, 1193, 1394, 1722, 1855, 1946, 2042, 2162, 2163
SduI GDGCHC 2 cut(s) 39, 969
SfaNI GCATC 7 cut(s) 271, 394, 424, 1330, 1412, 1755, 2029
SfcI CTRYAG 1 cut(s) 750
Sfr274I CTCGAG 1 cut(s) 86
SinI GGWCC 1 cut(s) 862
SlaI CTCGAG 1 cut(s) 86
SmaI CCCGGG 1 cut(s) 2163
SmlI CTYRAG 2 cut(s) 86, 266
SmoI CTYRAG 2 cut(s) 86, 266
SnaBI TACGTA 1 cut(s) 1830
SphI GCATGC 2 cut(s) 1215, 1595
SseBI AGGCCT 1 cut(s) 2137
SsiI CCGC 7 cut(s) 29, 50, 146, 185, 199, 1240, 1788
SspI AATATT 1 cut(s) 352
SspMI CTAG 5 cut(s) 1044, 1062, 1199, 2145, 2151
SstI GAGCTC 1 cut(s) 969
StuI AGGCCT 1 cut(s) 2137
StyD4I CCNGG 9 cut(s) 45, 1191, 1392, 1720, 1853, 1944, 2040, 2160, 2161
TaaI ACNGT 9 cut(s) 19, 118, 235, 595, 605, 1365, 1603, 1822, 1998
TaiI ACGT 3 cut(s) 817, 1127, 1832
TaqI TCGA 3 cut(s) 87, 1068, 1465
TatI WGTACW 1 cut(s) 1598
TauI GCSGC 1 cut(s) 1790
Tru1I TTAA 7 cut(s) 291, 660, 668, 818, 978, 1742, 1814
Tru9I TTAA 7 cut(s) 291, 660, 668, 818, 978, 1742, 1814
TscAI CASTG 7 cut(s) 22, 253, 598, 610, 1085, 1924, 1987
TseFI GTSAC 2 cut(s) 1538, 1915
Tsp45I GTSAC 2 cut(s) 1538, 1915
TspDTI ATGAA 7 cut(s) 560, 819, 975, 1196, 1253, 1521, 2109
TspGWI ACGGA 4 cut(s) 182, 959, 1262, 1819
TspMI CCCGGG 1 cut(s) 2161
TspRI CASTG 7 cut(s) 22, 253, 598, 610, 1085, 1924, 1987
Tth111I GACNNNGTC 1 cut(s) 1107
VpaK11BI GGWCC 1 cut(s) 862
XapI RAATTY 4 cut(s) 518, 806, 1640, 1797
XbaI TCTAGA 2 cut(s) 1198, 2144
XceI RCATGY 3 cut(s) 1215, 1595, 1900
XhoI CTCGAG 1 cut(s) 86
XmaI CCCGGG 1 cut(s) 2161
XmiI GTMKAC 1 cut(s) 1367
XspI CTAG 5 cut(s) 1044, 1062, 1199, 2145, 2151
ZrmI AGTACT 1 cut(s) 1600
Zsp2I ATGCAT 2 cut(s) 1213, 1217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.