MD13G1020500.v1.1

Protein VAC14 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012295
Physical Location & Seq
Forward (+)
1288610 .. 1295596
6987 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1020500.v1.1.491

Sequence Viewer

Length: 2187 bp
ATGGCCGACGGTATTTCAGTGATTCCCGCGGGCGTCCTCCGCAACCTCGCGGACAAGCTCTATGAGAAGCGCAAGAATGCCGCTCTTGAGGTTGAGGGAATAGTGAAGCAGCTCACGACGGCTGGGGAGCATGATAAGATTACGGCGGCGATCAATTTGCTCACTACGGAATTCACTTACTCGCCGCAAGCCAACCACCGGAAGGGAGGATTGATAGGATTAGCTGCTGCAACAGTTGGGTTGACCAGTGAAGCGGCTCAGCATCTTGAGCAAATTGTGCCCCCTGTGCTTAATTCCTTTTCGGACCAAGATAGCAGAGTTCGTTATTATGCTTGTGAAGCTCTATATAACATTGCAAAGGTTGTAAGAGGAGATTTTATCATATTCTTTAATGATATATTTGATGCCTTGTGCAAGCTTTCAGCAGACTCAGATGCTAATGTACAAAGTGCTGCTCATCTTTTAGATCGGCTTGTAAAGGATATTGTTACTGAAAGTGATCAATTCAGCATTGAAGAATTTATTCCACTGTTGAGGGAGCGTATGAATGTCCTGAATCCCTATGTCCGTCAGTTTTTGGTTGGATGGATCACTGTCTTAGACAGTGTTCCAGATATTGACATGCTGGGATTTCTTCCTGATTTTCTTGATGGCTTGTTCAATATGTTAAGTGATTCAAGCCATGAGATTAGACAACAAGCTGATTCAGCACTTTCAGAGTTTCTCCAGGAGATCAAGAACTCCCCATCTGTAGATTATGGTCGCATGGCTGAAATACTGGTACAGAGAGCTGATTCACCGGATGAATTTACTCGGTTAACAGCTATCACATGGATAAATGAATTTGTAAAACTTGGTGGGGACCAGCTTGTTCCCTATTATGCTGATATTCTGGGAGCAATTCTACCCTGCATATCTGATAAAGAAGAGAAAATTAGAGTGGTTGCTCGTGAAACCAATGAGGAGCTTCGTGCCATTAAGGCTGATCCAGCTGAAGGATTTGACGTTGGAGCAATCCTCTCTATTGCAAGGAGGCAATTATCTAGTGAATGGGAGGCTACTAGAATCGAAGCACTGCACTGGATTTCAAACCTTTTAAACAGATATCGCGCTGAGGTTCTAACTTTTCTGAACGAAATATTTGACACCCTTTTGGGAGCACTCTCAGATGCATCTGATCAGGTGGTTCTCTTGGTCCTAGAAGTTCATGCATGCATAGCAAAAGATCCACAGCACTTTCGCCAGCTTGTTGTGTTCCTTGTGCATAATTTTCGTGTGGACAACTCTCTTCTAGAGAAACGTGGTGCGTTGATTATTCGCCGACTTTGTGTGCTTTTAGATGCTGAAAGGGTCTACCGAGAACTGTCTACCATACTAGAAGGAGAGTCAGACCTGGATTTTGCATCTATTATGGTTCAGGCTTTGAACTTGATTTTACTTACATCCTCCGAGTTGGCTGAGCTTCGAGATCTTTTGAAAAGGTCACTTGTGAATCCTTCTGGGAAAGACTTATTTGTTTCCTTATATGCTTCATGGTGCCATTCACCCATGGCAATTATAAGCCTCTGCTTATTAGCTCAGACATATCAGCATGCAAGTACTGTGATTCAGTCTCTGGTTGAAGAAGATATTAATGTCAAGTTTTTGGTCCAGCTGGATAAATTGATTCGGTTACTAGAAACTCCAATCTTTGCGTATCTTAGATTACAGCTTCTTGAACCTGGAAGACATATATGGTTGTTAAAAGCCTTATATGGTCTTCTGATGTTGCTTCCTCAGCAAAGTGCCGCCTTTAAAATTCTGAGGACGCGTTTAAAAACTGTACCTTCATACTCATTCGACAGTGAGCAACTCAGAAGAACTTCTTCAGGGAATCCCCATCAGATTCTACATCACATGCCAAGTGCCAGTAGTTCGCAGATCATTGAAGACGGTGACATAAACCAGGCCAGTAAGAGTTCTCATAATGGAATTAACTTTACTTTAAGACTACGGCAGTTTGAGCAAATGCAGCAGCAACATCGTCAAAATGCAAAGCTGCAGGCACAAACACAAAGAACTTCCACAGCTTCTTCAACACCGAAGGATGTGCAGAGACCAGAAGAACAAAGCCATAGCCGATCTGCATCAGACGTGATTAGGGCGCCTTCCAGATCTCGGAGGGCCCCGGGGCAGTTACAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05166 ko05203 map05166 map05203
Pfam Domains
Protein Families
Vac14_Fab1_bd Vac14_Fig4_bd

Protein Analysis

729

Amino Acids

81.81

Weight (kDa)

5.6

Isoelectric Point (pI)

48.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOR1L1_N PF24714 15 - 240 7.3e-06 TORTIFOLIA1 N-terminal
Vac14_Fab1_bd PF12755 65 - 161 6.6e-41 Vacuolar 14 Fab1-binding region
HEAT_EF3_N PF24987 239 - 395 2.6e-07 EF3 N-terminal HEAT repeat
Vac14_Fig4_bd PF11916 429 - 608 1.9e-72 Vacuolar protein 14 C-terminal Fig4p binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012295)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01690 AT2G01690
fragaria_vesca FvH4_4g33570 FvH4_4g33570
malus_domestica MD13G1020500.v1.1 MD16G1018900.v1.1
prunus_persica Prupe.1G333200_v2.0.a1
pyrus_communis pycom13g01730 pycom16g01700
rosa_chinensis RchiOBHm_Chr4g0443071
rosa_laevigata RLG00000005956
rosa_multiflora Rmu_sc0008069.1_g000004
rosa_roxburghii Rroxscaffold_5G00383700
rosa_rugosa Rorug04G0344200 Rorug04G0344200
rosa_samantha Rh4BG410000 Rh4CG425500 Rh4DG404900
rosa_wichuraiana Rw4G034340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1559, 2185
AccB1I GGYRCC 2 cut(s) 1536, 2143
AccBSI CCGCTC 1 cut(s) 83
AccI GTMKAC 2 cut(s) 1353, 1367
AccII CGCG 4 cut(s) 29, 50, 1110, 1810
AciI CCGC 9 cut(s) 27, 29, 40, 50, 81, 146, 185, 254, 1788
AclWI GGATC 3 cut(s) 596, 980, 1220
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 5 cut(s) 170, 518, 806, 842, 1797
AcuI CTGAAG 2 cut(s) 1014, 1851
AcyI GRCGYC 2 cut(s) 33, 2144
AfaI GTAC 4 cut(s) 444, 783, 1600, 1824
AfiI CCNNNNNNNGG 4 cut(s) 198, 202, 995, 2157
AflIII ACRYGT 1 cut(s) 1808
AjiI CACGTC 1 cut(s) 2134
AjnI CCWGG 4 cut(s) 726, 1392, 1720, 1944
Alw21I GWGCWC 1 cut(s) 1162
Alw26I GTCTC 2 cut(s) 1617, 2089
AlwI GGATC 3 cut(s) 596, 980, 1220
AlwNI CAGNNNCTG 1 cut(s) 1615
Ama87I CYCGRG 1 cut(s) 2167
AoxI GGCC 3 cut(s) 3, 1947, 2163
ApaI GGGCCC 1 cut(s) 2167
ApeKI GCWGC 7 cut(s) 109, 224, 227, 452, 2011, 2014, 2038
ApoI RAATTY 5 cut(s) 170, 518, 806, 842, 1797
ArsI GACNNNNNNTTYG 2 cut(s) 1382, 1414
AseI ATTAAT 1 cut(s) 1632
Asp700I GAANNNNTTC 3 cut(s) 522, 1861, 1864
AspLEI GCGC 3 cut(s) 72, 1112, 2146
AspS9I GGNCC 6 cut(s) 304, 862, 1195, 1648, 2163, 2164
AsuC2I CCSGG 2 cut(s) 2168, 2169
AsuHPI GGTGA 3 cut(s) 789, 1536, 1946
AvaI CYCGRG 1 cut(s) 2167
AvaII GGWCC 4 cut(s) 304, 862, 1195, 1648
BaeGI GKGCMC 2 cut(s) 282, 2167
BanI GGYRCC 2 cut(s) 1536, 2143
BanII GRGCYC 1 cut(s) 2167
BauI CACGAG 1 cut(s) 948
BbsI GAAGAC 3 cut(s) 1732, 1751, 1935
Bbv12I GWGCWC 1 cut(s) 1162
BbvCI CCTCAGC 2 cut(s) 1113, 1776
BbvI GCAGC 7 cut(s) 121, 211, 214, 439, 2023, 2025, 2026
BccI CCATC 4 cut(s) 579, 644, 754, 1887
BceAI ACGGC 3 cut(s) 135, 159, 2009
BcgI CGANNNNNNTGC 8 cut(s) 139, 173, 1253, 1287, 2003, 2037, 2071, 2105
BciT130I CCWGG 4 cut(s) 728, 1394, 1722, 1946
BclI TGATCA 2 cut(s) 499, 1177
BcnI CCSGG 2 cut(s) 2168, 2169
BcoDI GTCTC 2 cut(s) 1617, 2089
BfaI CTAG 6 cut(s) 1044, 1062, 1199, 1292, 1376, 1676
BfmI CTRYAG 2 cut(s) 750, 2039
BfoI RGCGCY 1 cut(s) 2147
BglI GCCNNNNNGGC 1 cut(s) 980
BglII AGATCT 2 cut(s) 1468, 2153
BlpI GCTNAGC 2 cut(s) 258, 1458
BmcAI AGTACT 1 cut(s) 1600
Bme1390I CCNGG 6 cut(s) 728, 1394, 1722, 1946, 2168, 2169
Bme18I GGWCC 4 cut(s) 304, 862, 1195, 1648
BmeT110I CYCGRG 1 cut(s) 2167
BmgBI CACGTC 1 cut(s) 2134
BmgT120I GGNCC 6 cut(s) 304, 862, 1195, 1648, 2163, 2164
BmiI GGNNCC 5 cut(s) 863, 1538, 2145, 2165, 2166
BmrFI CCNGG 6 cut(s) 728, 1394, 1722, 1946, 2168, 2169
BmsI GCATC 8 cut(s) 271, 394, 424, 1159, 1181, 1330, 1412, 2135
BpiI GAAGAC 3 cut(s) 1732, 1751, 1935
BplI GAGNNNNNCTC 2 cut(s) 1002, 1034
BpmI CTGGAG 1 cut(s) 710
Bpu10I CCTNAGC 2 cut(s) 1113, 1776
Bpu1102I GCTNAGC 2 cut(s) 258, 1458
BpuEI CTTGAG 2 cut(s) 107, 287
BpuMI CCSGG 2 cut(s) 2168, 2169
BsaBI GATNNNNATC 1 cut(s) 2125
BsaHI GRCGYC 2 cut(s) 33, 2144
BsaI GGTCTC 1 cut(s) 2089
BsaJI CCNNGG 5 cut(s) 27, 1548, 2166, 2167, 2168
BsaWI WCCGGW 2 cut(s) 198, 799
Bsc4I CCNNNNNNNGG 4 cut(s) 198, 202, 995, 2157
Bse1I ACTGG 5 cut(s) 246, 783, 1085, 1908, 1950
Bse3DI GCAATG 1 cut(s) 351
Bse8I GATNNNNATC 1 cut(s) 2125
BseBI CCWGG 4 cut(s) 728, 1394, 1722, 1946
BseDI CCNNGG 5 cut(s) 27, 1548, 2166, 2167, 2168
BseGI GGATG 4 cut(s) 590, 808, 1442, 2092
BseJI GATNNNNATC 1 cut(s) 2125
BseLI CCNNNNNNNGG 4 cut(s) 198, 202, 995, 2157
BseMI GCAATG 1 cut(s) 351
BseMII CTCAG 9 cut(s) 272, 444, 1104, 1179, 1449, 1592, 1790, 1793, 1867
BseNI ACTGG 5 cut(s) 246, 783, 1085, 1908, 1950
BseRI GAGGAG 2 cut(s) 384, 977
BseSI GKGCMC 2 cut(s) 282, 2167
BseXI GCAGC 7 cut(s) 121, 211, 214, 439, 2023, 2025, 2026
BseYI CCCAGC 2 cut(s) 122, 625
BsgI GTGCAG 2 cut(s) 1061, 2111
Bsh1236I CGCG 4 cut(s) 29, 50, 1110, 1810
BshFI GGCC 3 cut(s) 5, 1949, 2165
BshNI GGYRCC 2 cut(s) 1536, 2143
BsiHKAI GWGCWC 1 cut(s) 1162
BsiHKCI CYCGRG 1 cut(s) 2167
BsiSI CCGG 3 cut(s) 199, 800, 2168
BslFI GGGAC 1 cut(s) 875
BslI CCNNNNNNNGG 4 cut(s) 198, 202, 995, 2157
BsmAI GTCTC 2 cut(s) 1617, 2089
BsmFI GGGAC 1 cut(s) 875
BsmI GAATGC 1 cut(s) 82
BsnI GGCC 3 cut(s) 5, 1949, 2165
Bso31I GGTCTC 1 cut(s) 2089
BsoBI CYCGRG 1 cut(s) 2167
Bsp120I GGGCCC 1 cut(s) 2163
Bsp1286I GDGCHC 3 cut(s) 282, 1162, 2167
Bsp1407I TGTACA 1 cut(s) 442
Bsp1720I GCTNAGC 2 cut(s) 258, 1458
Bsp19I CCATGG 1 cut(s) 1548
BspACI CCGC 9 cut(s) 27, 29, 40, 50, 81, 146, 185, 254, 1788
BspANI GGCC 3 cut(s) 5, 1949, 2165
BspCNI CTCAG 9 cut(s) 271, 443, 1105, 1178, 1450, 1591, 1789, 1794, 1866
BspFNI CGCG 4 cut(s) 29, 50, 1110, 1810
BspLI GGNNCC 5 cut(s) 863, 1538, 2145, 2165, 2166
BspMAI CTGCAG 1 cut(s) 2043
BspPI GGATC 3 cut(s) 596, 980, 1220
BspT107I GGYRCC 2 cut(s) 1536, 2143
BspTNI GGTCTC 1 cut(s) 2089
BsrBI CCGCTC 1 cut(s) 83
BsrDI GCAATG 1 cut(s) 351
BsrGI TGTACA 1 cut(s) 442
BsrI ACTGG 5 cut(s) 246, 783, 1085, 1908, 1950
BssECI CCNNGG 5 cut(s) 27, 1548, 2166, 2167, 2168
BssNI GRCGYC 2 cut(s) 33, 2144
BssSI CACGAG 1 cut(s) 948
BssT1I CCWWGG 1 cut(s) 1548
Bst2BI CACGAG 1 cut(s) 948
Bst2UI CCWGG 4 cut(s) 728, 1394, 1722, 1946
Bst6I CTCTTC 2 cut(s) 921, 1293
BstACI GRCGYC 2 cut(s) 33, 2144
BstAPI GCANNNNNTGC 1 cut(s) 277
BstAUI TGTACA 1 cut(s) 442
BstC8I GCNNGC 7 cut(s) 31, 189, 416, 1213, 1244, 1593, 2043
BstDSI CCRYGG 2 cut(s) 27, 1548
BstF5I GGATG 4 cut(s) 590, 808, 1442, 2092
BstFNI CGCG 4 cut(s) 29, 50, 1110, 1810
BstH2I RGCGCY 1 cut(s) 2147
BstHHI GCGC 3 cut(s) 72, 1112, 2146
BstMAI GTCTC 2 cut(s) 1617, 2089
BstNI CCWGG 4 cut(s) 728, 1394, 1722, 1946
BstNSI RCATGY 4 cut(s) 625, 1215, 1595, 1900
BstSCI CCNGG 6 cut(s) 726, 1392, 1720, 1944, 2166, 2167
BstSFI CTRYAG 2 cut(s) 750, 2039
BstSLI GKGCMC 2 cut(s) 282, 2167
BstUI CGCG 4 cut(s) 29, 50, 1110, 1810
BstV1I GCAGC 7 cut(s) 121, 211, 214, 439, 2023, 2025, 2026
BstV2I GAAGAC 3 cut(s) 1732, 1751, 1935
BstX2I RGATCY 3 cut(s) 1225, 1468, 2153
BstYI RGATCY 3 cut(s) 1225, 1468, 2153
BsuRI GGCC 3 cut(s) 5, 1949, 2165
BtgI CCRYGG 2 cut(s) 27, 1548
BtrI CACGTC 1 cut(s) 2134
BtsCI GGATG 4 cut(s) 590, 808, 1442, 2092
BtsI GCAGTG 1 cut(s) 1073
BtsIMutI CAGTG 8 cut(s) 24, 253, 527, 591, 610, 1073, 1078, 1849
Cac8I GCNNGC 7 cut(s) 31, 189, 416, 1213, 1244, 1593, 2043
CaiI CAGNNNCTG 1 cut(s) 1615
CfoI GCGC 3 cut(s) 72, 1112, 2146
Cfr13I GGNCC 6 cut(s) 304, 862, 1195, 1648, 2163, 2164
Cfr42I CCGCGG 1 cut(s) 30
Cfr9I CCCGGG 1 cut(s) 2167
CseI GACGC 2 cut(s) 22, 1816
Csp6I GTAC 4 cut(s) 443, 782, 1599, 1823
CviQI GTAC 4 cut(s) 443, 782, 1599, 1823
DinI GGCGCC 1 cut(s) 2145
DraI TTTAAA 3 cut(s) 1098, 1795, 1815
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 921, 1293
EarI CTCTTC 2 cut(s) 921, 1293
Eco130I CCWWGG 1 cut(s) 1548
Eco24I GRGCYC 1 cut(s) 2167
Eco31I GGTCTC 1 cut(s) 2089
Eco32I GATATC 1 cut(s) 1106
Eco47I GGWCC 4 cut(s) 304, 862, 1195, 1648
Eco57I CTGAAG 2 cut(s) 1014, 1851
Eco88I CYCGRG 1 cut(s) 2167
EcoO109I RGGNCCY 2 cut(s) 2163, 2164
EcoRI GAATTC 1 cut(s) 170
EcoRII CCWGG 4 cut(s) 726, 1392, 1720, 1944
EcoRV GATATC 1 cut(s) 1106
EcoT14I CCWWGG 1 cut(s) 1548
EcoT22I ATGCAT 3 cut(s) 1174, 1213, 1217
EcoT38I GRGCYC 1 cut(s) 2167
EgeI GGCGCC 1 cut(s) 2145
EheI GGCGCC 1 cut(s) 2145
ErhI CCWWGG 1 cut(s) 1548
FalI AAGNNNNNCTT 2 cut(s) 1849, 1881
FaqI GGGAC 1 cut(s) 875
FauI CCCGC 2 cut(s) 22, 34
FbaI TGATCA 2 cut(s) 499, 1177
FblI GTMKAC 2 cut(s) 1353, 1367
FokI GGATG 4 cut(s) 597, 815, 1429, 2099
FriOI GRGCYC 1 cut(s) 2167
FspBI CTAG 6 cut(s) 1044, 1062, 1199, 1292, 1376, 1676
GlaI GCGC 3 cut(s) 71, 1111, 2145
GsaI CCCAGC 2 cut(s) 126, 629
GsuI CTGGAG 1 cut(s) 710
HaeII RGCGCY 1 cut(s) 2147
HaeIII GGCC 3 cut(s) 5, 1949, 2165
HapII CCGG 3 cut(s) 199, 800, 2168
HgaI GACGC 2 cut(s) 22, 1816
HhaI GCGC 3 cut(s) 72, 1112, 2146
Hin1I GRCGYC 2 cut(s) 33, 2144
Hin6I GCGC 3 cut(s) 70, 1110, 2144
HinP1I GCGC 3 cut(s) 70, 1110, 2144
HincII GTYRAC 2 cut(s) 243, 819
HindII GTYRAC 2 cut(s) 243, 819
HindIII AAGCTT 1 cut(s) 416
HpaI GTTAAC 1 cut(s) 819
HpaII CCGG 3 cut(s) 199, 800, 2168
HphI GGTGA 3 cut(s) 789, 1536, 1946
Hpy166II GTNNAC 5 cut(s) 243, 819, 1279, 1354, 1368
Hpy8I GTNNAC 5 cut(s) 243, 819, 1279, 1354, 1368
Hpy99I CGWCG 2 cut(s) 11, 121
HpyAV CCTTC 7 cut(s) 196, 989, 1373, 1506, 1836, 2077, 2157
HpyCH4IV ACGT 3 cut(s) 1005, 1300, 2133
HpySE526I ACGT 3 cut(s) 1005, 1300, 2133
Hsp92I GRCGYC 2 cut(s) 33, 2144
HspAI GCGC 3 cut(s) 70, 1110, 2144
KasI GGCGCC 1 cut(s) 2143
Ksp22I TGATCA 2 cut(s) 499, 1177
KspAI GTTAAC 1 cut(s) 819
KspI CCGCGG 1 cut(s) 30
LmnI GCTCC 6 cut(s) 127, 538, 896, 964, 1010, 1157
Lsp1109I GCAGC 7 cut(s) 121, 211, 214, 439, 2023, 2025, 2026
LweI GCATC 8 cut(s) 271, 394, 424, 1159, 1181, 1330, 1412, 2135
MaeI CTAG 6 cut(s) 1044, 1062, 1199, 1292, 1376, 1676
MaeII ACGT 3 cut(s) 1005, 1300, 2133
MaeIII GTNAC 5 cut(s) 487, 1482, 1671, 1934, 2175
MbiI CCGCTC 1 cut(s) 83
MflI RGATCY 3 cut(s) 1225, 1468, 2153
MhlI GDGCHC 3 cut(s) 282, 1162, 2167
MluI ACGCGT 1 cut(s) 1808
Mly113I GGCGCC 1 cut(s) 2144
MlyI GAGTC 2 cut(s) 422, 1394
MmeI TCCRAC 2 cut(s) 562, 988
Mph1103I ATGCAT 3 cut(s) 1174, 1213, 1217
MroXI GAANNNNTTC 3 cut(s) 522, 1861, 1864
MspA1I CMGCKG 3 cut(s) 29, 992, 1654
MspI CCGG 3 cut(s) 199, 800, 2168
MspR9I CCNGG 6 cut(s) 728, 1394, 1722, 1946, 2168, 2169
Mva1269I GAATGC 1 cut(s) 82
MvaI CCWGG 4 cut(s) 728, 1394, 1722, 1946
MvnI CGCG 4 cut(s) 29, 50, 1110, 1810
NarI GGCGCC 1 cut(s) 2144
NciI CCSGG 2 cut(s) 2168, 2169
NcoI CCATGG 1 cut(s) 1548
NlaIV GGNNCC 5 cut(s) 863, 1538, 2145, 2165, 2166
NmuCI GTSAC 2 cut(s) 1482, 1934
NsiI ATGCAT 3 cut(s) 1174, 1213, 1217
NspI RCATGY 4 cut(s) 625, 1215, 1595, 1900
PaeI GCATGC 2 cut(s) 1215, 1595
PctI GAATGC 1 cut(s) 82
PdmI GAANNNNTTC 3 cut(s) 522, 1861, 1864
PfoI TCCNGGA 1 cut(s) 726
PleI GAGTC 2 cut(s) 422, 1393
PluTI GGCGCC 1 cut(s) 2147
PpsI GAGTC 2 cut(s) 422, 1393
PshBI ATTAAT 1 cut(s) 1632
PsiI TTATAA 2 cut(s) 1559, 2185
Psp6I CCWGG 4 cut(s) 726, 1392, 1720, 1944
PspFI CCCAGC 2 cut(s) 122, 625
PspGI CCWGG 4 cut(s) 726, 1392, 1720, 1944
PspN4I GGNNCC 5 cut(s) 863, 1538, 2145, 2165, 2166
PspOMI GGGCCC 1 cut(s) 2163
PspPI GGNCC 6 cut(s) 304, 862, 1195, 1648, 2163, 2164
PstI CTGCAG 1 cut(s) 2043
PstNI CAGNNNCTG 1 cut(s) 1615
PsuI RGATCY 3 cut(s) 1225, 1468, 2153
PvuII CAGCTG 2 cut(s) 992, 1654
RsaI GTAC 4 cut(s) 444, 783, 1600, 1824
RsaNI GTAC 4 cut(s) 443, 782, 1599, 1823
SacII CCGCGG 1 cut(s) 30
Sau96I GGNCC 6 cut(s) 304, 862, 1195, 1648, 2163, 2164
ScaI AGTACT 1 cut(s) 1600
SchI GAGTC 2 cut(s) 422, 1394
ScrFI CCNGG 6 cut(s) 728, 1394, 1722, 1946, 2168, 2169
SduI GDGCHC 3 cut(s) 282, 1162, 2167
SfaNI GCATC 8 cut(s) 271, 394, 424, 1159, 1181, 1330, 1412, 2135
SfcI CTRYAG 2 cut(s) 750, 2039
SfoI GGCGCC 1 cut(s) 2145
Sfr303I CCGCGG 1 cut(s) 30
SgrBI CCGCGG 1 cut(s) 30
SinI GGWCC 4 cut(s) 304, 862, 1195, 1648
SmaI CCCGGG 1 cut(s) 2169
SmlI CTYRAG 2 cut(s) 86, 266
SmoI CTYRAG 2 cut(s) 86, 266
SphI GCATGC 2 cut(s) 1215, 1595
SsiI CCGC 9 cut(s) 27, 29, 40, 50, 81, 146, 185, 254, 1788
SspDI GGCGCC 1 cut(s) 2143
SspI AATATT 1 cut(s) 1140
SspMI CTAG 6 cut(s) 1044, 1062, 1199, 1292, 1376, 1676
StyD4I CCNGG 6 cut(s) 726, 1392, 1720, 1944, 2166, 2167
StyI CCWWGG 1 cut(s) 1548
TaiI ACGT 3 cut(s) 1008, 1303, 2136
TaqI TCGA 3 cut(s) 1068, 1465, 1839
TatI WGTACW 2 cut(s) 442, 1598
TauI GCSGC 5 cut(s) 83, 149, 187, 257, 1790
TscAI CASTG 8 cut(s) 24, 253, 534, 598, 610, 1080, 1085, 1849
TseFI GTSAC 2 cut(s) 1482, 1934
TseI GCWGC 7 cut(s) 109, 224, 227, 452, 2011, 2014, 2038
Tsp45I GTSAC 2 cut(s) 1482, 1934
TspDTI ATGAA 6 cut(s) 560, 819, 855, 1196, 1521, 1818
TspGWI ACGGA 2 cut(s) 182, 557
TspMI CCCGGG 1 cut(s) 2167
TspRI CASTG 8 cut(s) 24, 253, 534, 598, 610, 1080, 1085, 1849
VpaK11BI GGWCC 4 cut(s) 304, 862, 1195, 1648
VspI ATTAAT 1 cut(s) 1632
XapI RAATTY 5 cut(s) 170, 518, 806, 842, 1797
XbaI TCTAGA 1 cut(s) 1291
XceI RCATGY 4 cut(s) 625, 1215, 1595, 1900
XmaI CCCGGG 1 cut(s) 2167
XmiI GTMKAC 2 cut(s) 1353, 1367
XmnI GAANNNNTTC 3 cut(s) 522, 1861, 1864
XspI CTAG 6 cut(s) 1044, 1062, 1199, 1292, 1376, 1676
ZrmI AGTACT 1 cut(s) 1600
Zsp2I ATGCAT 3 cut(s) 1174, 1213, 1217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.