Rh4CG425500

Protein VAC14 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0012295
Physical Location & Seq
Forward (+)
66855325 .. 66864020
8696 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG425500.1

Sequence Viewer

Length: 2178 bp
ATGGCCGACGCGCTTACTGTGATTCCGGCCGGCGTGCTCCGGAACCTGGCGGACAAGCTCTATGAGAAGCGCAAGAATGCCGCTCTCGAGGTTGAGGGAATCGTGAAGCAGCTCACGACGGCTGGGGATCATGATAAGATTACGGCCGTGATCAATTTGCTCACTACGGAATTCACTTATTCACCGCAGGCGAATCACCGGAAGGGAGGGTTGATAGGTTTAGCTGCTGCAACAGTTGGGTTGACCAGCGAAGCTGCTCAGCATCTTGAGCAAATTGTGCCTCCTGTGCTTAATTCCTTTTCTGACCAAGATAGCAGAGTTCGTTATTATGCTTGTGAAGCACTATACAACATTGCAAAGGTTGTACGAGGAGATTTTATCATATTCTTTAATCAGATTTTTGATGCCTTGTGCAAGCTTTCAGCAGACTCAGATGCTAATGTACAAAGTGCTGCTCATCTTTTAGATCGACTTGTAAAGCAGGATATTGTTACTGAAAGCGACCAATTCAGCATTGAAGAATTTATACCATTACTGAGGGAGCGCATGAATGTTCTAAATCCCTATGTCCGCCAGTTTTTGGTAGGATGGATCACTGTTCTAGACAGCGTTCCAGATATTGATATGCTGGGTTTCCTTCCTGATTTTCTTGATGGTTTGTTTAATATGTTAAGTGATTCAAGCCATGAGATAAGACAACAAGCTGATTCAGCTCTTTCAGAGTTTCTCCAAGAGATCAAGAACTCCCCATCTGTAGATTATGGTCGCATGGCTGAAATACTGGTGCAGAGGGCTGCTTCTCCGGATGAATTTACTCGGTTAACAGCTATCACATGGATAAATGAGTTTGTAAAACTTGGTGGGGACCAGCTTGTTCCCTATTATGCTGATATATTGGGAGCAATTCTGCCCTGCATATCCGATAAAGAAGAGAAAATCAGAGTGGTTGCTCGCGAAACCAATGAAGAGCTCCGTGCCATTAAGGCTGATCCAGCTGAAGGGTTTGATGTTGGAGCAATCCTCTCTATTGCAAGGATGCAACTATCTAGTGAATGGGAGGCTACTAGAATTGAAGCATTGCACTGGATTTCCAACCTTCTAAACAGACATCGTTATGAGGTGTTGAATTATCTGAATGACATATTTGACACCCTTTTGGAGGCACTTTCAGATCCTTCTGATAAGGTGGTTCTCTTGGTTCTAGAGGTTCATGCATGCATAGCGAAAGACCTACTACACTTCCGCCAGCTTGTTGTCTTTCTTGTTCATAATTTCCGTGTAGATAATTCTCTTCTGGAGAAGCGTGGTGCTTTGATAATTCGCCGACTCTGTGTGCTTTTAGATGCTGAAAGGGTCTACCGAGAACTGTCTACTATACTAGAAGGAGAATCAGACCTGGATTTTGCATCTATTATGGTTCAGGCTTTGAACTTGATTTTACTTACATCCTCCGAGTTGTCTGACCTACGAGATCTTTTGAAACAATCACTTGTGAGTCCATCAGGGAAGGACTTATTTGTTTCTTTATATGCTTCATGGTGTCACTCACCCATGGCAATTATAAGCCTCTGCTTGTTATCTCAGACATATCAGCATGCAAGTACTGTGATTCAGTCTCTGGTGGAGGAAGATATTAATGTCAAATTTTTAGTCCAGCTGGATAAATTGATTCGGTTACTGGAAACTCCAATCTTTGCGTATCTTAGATTACAGCTTCTGGAACCTGGAAGGCATATGTGGCTGTTAAAAGCCCTGTATGGTCTTCTGATGTTACTTCCCCAGCAAAGTGCCGCCTTTAAAATTCTACGGACTCGTTTAAAAACTGTACCTTCGTACTCATTTAGTGGTGAGCAACCGAGGCGAACTTCTTCAGGGAATTCCTATCTGACTCTACAACACATGTCAAGTGGATCACAGGTCACTGAGGATGGTGACATAAACCAGGACAATAAGTCTTCCCATCATGGAATTAACTTCACTGCAAAACTACAGCAGTTTGAGCAAATGCAGCAGCAGCATCGCCAGCATGCAAAAGTTCAGGCACAATCAGGCATTAATTCCACATCTTCCCCAATGCAACAGGGTGTGCAAAGACCTGAAGAACTCATCCGATCTTCATCAGACATGAATAGGCCTCCTTCTAGATCTAGGAGGGGACAAGGGCAGTTACAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05166 ko05203 map05166 map05203
Pfam Domains
Protein Families
Vac14_Fab1_bd Vac14_Fig4_bd

Protein Analysis

725

Amino Acids

81.56

Weight (kDa)

5.48

Isoelectric Point (pI)

46.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOR1L1_N PF24714 15 - 241 4e-06 TORTIFOLIA1 N-terminal
Vac14_Fab1_bd PF12755 65 - 161 2e-40 Vacuolar 14 Fab1-binding region
HEAT_EF3_N PF24987 256 - 396 5.6e-07 EF3 N-terminal HEAT repeat
Vac14_Fig4_bd PF11916 430 - 609 1.8e-72 Vacuolar protein 14 C-terminal Fig4p binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012295)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01690 AT2G01690
fragaria_vesca FvH4_4g33570 FvH4_4g33570
malus_domestica MD13G1020500.v1.1 MD16G1018900.v1.1
prunus_persica Prupe.1G333200_v2.0.a1
pyrus_communis pycom13g01730 pycom16g01700
rosa_chinensis RchiOBHm_Chr4g0443071
rosa_laevigata RLG00000005956
rosa_multiflora Rmu_sc0008069.1_g000004
rosa_roxburghii Rroxscaffold_5G00383700
rosa_rugosa Rorug04G0344200 Rorug04G0344200
rosa_samantha Rh4BG410000 Rh4CG425500 Rh4DG404900
rosa_wichuraiana Rw4G034340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1562, 2176
AccB7I CCANNNNNTGG 1 cut(s) 580
AccBSI CCGCTC 1 cut(s) 83
AccI GTMKAC 2 cut(s) 1356, 1370
AccII CGCG 2 cut(s) 11, 954
AccIII TCCGGA 2 cut(s) 39, 802
AciI CCGC 6 cut(s) 50, 81, 185, 571, 1243, 1791
AclWI GGATC 5 cut(s) 135, 599, 983, 1166, 1918
AcoI YGGCCR 3 cut(s) 3, 27, 144
AcsI RAATTY 6 cut(s) 170, 521, 809, 1643, 1800, 1876
AcuI CTGAAG 3 cut(s) 1017, 1854, 2118
AfaI GTAC 5 cut(s) 366, 444, 1603, 1827, 1835
AfiI CCNNNNNNNGG 4 cut(s) 46, 580, 998, 1159
AflIII ACRYGT 1 cut(s) 1899
AgsI TTSAA 6 cut(s) 518, 681, 1073, 1126, 1429, 1480
AhdI GACNNNNNGTC 1 cut(s) 1951
AjnI CCWGG 4 cut(s) 45, 1395, 1723, 1941
Alw21I GWGCWC 2 cut(s) 39, 972
Alw26I GTCTC 1 cut(s) 1620
AlwI GGATC 5 cut(s) 135, 599, 983, 1166, 1918
AlwNI CAGNNNCTG 2 cut(s) 1618, 1717
Ama87I CYCGRG 1 cut(s) 86
Aor13HI TCCGGA 2 cut(s) 39, 802
AoxI GGCC 4 cut(s) 3, 27, 144, 2132
ApeKI GCWGC 9 cut(s) 109, 224, 227, 254, 452, 794, 2008, 2011, 2014
ApoI RAATTY 6 cut(s) 170, 521, 809, 1643, 1800, 1876
ArsI GACNNNNNNTTYG 4 cut(s) 1385, 1417, 1635, 1667
AseI ATTAAT 2 cut(s) 1635, 2055
Asp700I GAANNNNTTC 1 cut(s) 1867
AspLEI GCGC 3 cut(s) 13, 72, 546
AspS9I GGNCC 1 cut(s) 865
AsuHPI GGTGA 5 cut(s) 174, 188, 1539, 1859, 1943
AvaI CYCGRG 1 cut(s) 86
AvaII GGWCC 1 cut(s) 865
BanII GRGCYC 1 cut(s) 972
BarI GAAGNNNNNNTAC 2 cut(s) 510, 542
BbsI GAAGAC 2 cut(s) 1754, 1947
Bbv12I GWGCWC 2 cut(s) 39, 972
BbvI GCAGC 9 cut(s) 121, 211, 214, 241, 439, 781, 2020, 2023, 2026
BccI CCATC 6 cut(s) 582, 647, 757, 1507, 1922, 1968
BceAI ACGGC 3 cut(s) 131, 135, 159
BcgI CGANNNNNNTGC 2 cut(s) 2000, 2034
BciT130I CCWGG 4 cut(s) 47, 1397, 1725, 1943
BclI TGATCA 1 cut(s) 150
BcoDI GTCTC 1 cut(s) 1620
BfaI CTAG 7 cut(s) 602, 1047, 1065, 1202, 1379, 2142, 2148
BfmI CTRYAG 2 cut(s) 753, 1988
BglI GCCNNNNNGGC 1 cut(s) 983
BglII AGATCT 2 cut(s) 1471, 2144
BlpI GCTNAGC 1 cut(s) 258
BmcAI AGTACT 1 cut(s) 1603
Bme1390I CCNGG 4 cut(s) 47, 1397, 1725, 1943
Bme18I GGWCC 1 cut(s) 865
BmeRI GACNNNNNGTC 1 cut(s) 1951
BmeT110I CYCGRG 1 cut(s) 86
BmgT120I GGNCC 1 cut(s) 865
BmiI GGNNCC 3 cut(s) 44, 866, 1722
BmrFI CCNGG 4 cut(s) 47, 1397, 1725, 1943
BmsI GCATC 7 cut(s) 271, 394, 424, 1026, 1333, 1415, 2026
BpiI GAAGAC 2 cut(s) 1754, 1947
BplI GAGNNNNNCTC 2 cut(s) 1005, 1037
BpmI CTGGAG 1 cut(s) 1316
Bpu1102I GCTNAGC 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 287
BsaBI GATNNNNATC 1 cut(s) 2116
BsaJI CCNNGG 2 cut(s) 1551, 1856
BsaWI WCCGGW 3 cut(s) 39, 198, 802
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 580, 998, 1159
Bse118I RCCGGY 1 cut(s) 29
Bse1I ACTGG 4 cut(s) 574, 786, 1088, 1683
Bse3DI GCAATG 2 cut(s) 351, 1076
Bse8I GATNNNNATC 1 cut(s) 2116
BseAI TCCGGA 2 cut(s) 39, 802
BseBI CCWGG 4 cut(s) 47, 1397, 1725, 1943
BseDI CCNNGG 2 cut(s) 1551, 1856
BseGI GGATG 6 cut(s) 593, 811, 1041, 1445, 1933, 2106
BseJI GATNNNNATC 1 cut(s) 2116
BseLI CCNNNNNNNGG 4 cut(s) 46, 580, 998, 1159
BseMI GCAATG 2 cut(s) 351, 1076
BseMII CTCAG 5 cut(s) 272, 444, 527, 1595, 1914
BseNI ACTGG 4 cut(s) 574, 786, 1088, 1683
BseRI GAGGAG 1 cut(s) 384
BseX3I CGGCCG 2 cut(s) 27, 144
BseXI GCAGC 9 cut(s) 121, 211, 214, 241, 439, 781, 2020, 2023, 2026
BseYI CCCAGC 3 cut(s) 122, 628, 1779
BsgI GTGCAG 1 cut(s) 806
Bsh1236I CGCG 2 cut(s) 11, 954
Bsh1285I CGRYCG 2 cut(s) 30, 147
BshFI GGCC 4 cut(s) 5, 29, 146, 2134
BsiEI CGRYCG 2 cut(s) 30, 147
BsiHKAI GWGCWC 2 cut(s) 39, 972
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 5 cut(s) 26, 30, 40, 199, 803
BslFI GGGAC 2 cut(s) 878, 2169
BslI CCNNNNNNNGG 4 cut(s) 46, 580, 998, 1159
BsmAI GTCTC 1 cut(s) 1620
BsmFI GGGAC 2 cut(s) 878, 2169
BsmI GAATGC 1 cut(s) 82
BsnI GGCC 4 cut(s) 5, 29, 146, 2134
BsoBI CYCGRG 1 cut(s) 86
Bsp1286I GDGCHC 2 cut(s) 39, 972
Bsp13I TCCGGA 2 cut(s) 39, 802
Bsp1407I TGTACA 1 cut(s) 442
Bsp1720I GCTNAGC 1 cut(s) 258
Bsp19I CCATGG 1 cut(s) 1551
Bsp68I TCGCGA 1 cut(s) 954
BspACI CCGC 6 cut(s) 50, 81, 185, 571, 1243, 1791
BspANI GGCC 4 cut(s) 5, 29, 146, 2134
BspCNI CTCAG 5 cut(s) 271, 443, 528, 1594, 1915
BspEI TCCGGA 2 cut(s) 39, 802
BspFNI CGCG 2 cut(s) 11, 954
BspHI TCATGA 1 cut(s) 130
BspLI GGNNCC 3 cut(s) 44, 866, 1722
BspPI GGATC 5 cut(s) 135, 599, 983, 1166, 1918
BspQI GCTCTTC 1 cut(s) 960
BsrBI CCGCTC 1 cut(s) 83
BsrDI GCAATG 2 cut(s) 351, 1076
BsrFI RCCGGY 1 cut(s) 29
BsrGI TGTACA 1 cut(s) 442
BsrI ACTGG 4 cut(s) 574, 786, 1088, 1683
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 2 cut(s) 1551, 1856
BssT1I CCWWGG 1 cut(s) 1551
Bst2UI CCWGG 4 cut(s) 47, 1397, 1725, 1943
Bst4CI ACNGT 6 cut(s) 19, 235, 598, 1368, 1606, 1825
Bst6I CTCTTC 3 cut(s) 924, 960, 1296
BstAPI GCANNNNNTGC 1 cut(s) 277
BstAUI TGTACA 1 cut(s) 442
BstDEI CTNAG 6 cut(s) 258, 430, 536, 1581, 1703, 1923
BstDSI CCRYGG 1 cut(s) 1551
BstENI CCTNNNNNAGG 1 cut(s) 1157
BstF5I GGATG 6 cut(s) 593, 811, 1041, 1445, 1933, 2106
BstFNI CGCG 2 cut(s) 11, 954
BstHHI GCGC 3 cut(s) 13, 72, 546
BstMAI GTCTC 1 cut(s) 1620
BstMCI CGRYCG 2 cut(s) 30, 147
BstNI CCWGG 4 cut(s) 47, 1397, 1725, 1943
BstNSI RCATGY 4 cut(s) 1218, 1598, 1903, 2030
BstSCI CCNGG 4 cut(s) 45, 1395, 1723, 1941
BstSFI CTRYAG 2 cut(s) 753, 1988
BstUI CGCG 2 cut(s) 11, 954
BstV1I GCAGC 9 cut(s) 121, 211, 214, 241, 439, 781, 2020, 2023, 2026
BstV2I GAAGAC 2 cut(s) 1754, 1947
BstX2I RGATCY 3 cut(s) 1171, 1471, 2144
BstYI RGATCY 3 cut(s) 1171, 1471, 2144
BstZI CGGCCG 2 cut(s) 27, 144
BsuRI GGCC 4 cut(s) 5, 29, 146, 2134
BtgI CCRYGG 1 cut(s) 1551
BtgZI GCGATG 1 cut(s) 2003
BtsCI GGATG 6 cut(s) 593, 811, 1041, 1445, 1933, 2106
BtsI GCAGTG 1 cut(s) 1977
BtsIMutI CAGTG 4 cut(s) 594, 1081, 1920, 1977
BtuMI TCGCGA 1 cut(s) 954
CaiI CAGNNNCTG 2 cut(s) 1618, 1717
CciI TCATGA 1 cut(s) 130
CfoI GCGC 3 cut(s) 13, 72, 546
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 1 cut(s) 865
CseI GACGC 1 cut(s) 17
Csp6I GTAC 5 cut(s) 365, 443, 1602, 1826, 1834
CviQI GTAC 5 cut(s) 365, 443, 1602, 1826, 1834
DdeI CTNAG 6 cut(s) 258, 430, 536, 1581, 1703, 1923
DraI TTTAAA 2 cut(s) 1798, 1818
DriI GACNNNNNGTC 1 cut(s) 1951
EaeI YGGCCR 3 cut(s) 3, 27, 144
EagI CGGCCG 2 cut(s) 27, 144
Eam1104I CTCTTC 3 cut(s) 924, 960, 1296
Eam1105I GACNNNNNGTC 1 cut(s) 1951
EarI CTCTTC 3 cut(s) 924, 960, 1296
EciI GGCGGA 3 cut(s) 65, 560, 1232
Ecl136II GAGCTC 1 cut(s) 970
EclXI CGGCCG 2 cut(s) 27, 144
Eco130I CCWWGG 1 cut(s) 1551
Eco147I AGGCCT 1 cut(s) 2134
Eco24I GRGCYC 1 cut(s) 972
Eco47I GGWCC 1 cut(s) 865
Eco52I CGGCCG 2 cut(s) 27, 144
Eco53kI GAGCTC 1 cut(s) 970
Eco57I CTGAAG 3 cut(s) 1017, 1854, 2118
Eco88I CYCGRG 1 cut(s) 86
EcoICRI GAGCTC 1 cut(s) 970
EcoNI CCTNNNNNAGG 1 cut(s) 1157
EcoRI GAATTC 2 cut(s) 170, 1876
EcoRII CCWGG 4 cut(s) 45, 1395, 1723, 1941
EcoT14I CCWWGG 1 cut(s) 1551
EcoT22I ATGCAT 2 cut(s) 1216, 1220
EcoT38I GRGCYC 1 cut(s) 972
ErhI CCWWGG 1 cut(s) 1551
FaqI GGGAC 2 cut(s) 878, 2169
FauNDI CATATG 1 cut(s) 1734
FbaI TGATCA 1 cut(s) 150
FblI GTMKAC 2 cut(s) 1356, 1370
FokI GGATG 6 cut(s) 600, 818, 1048, 1432, 1940, 2093
FriOI GRGCYC 1 cut(s) 972
FspBI CTAG 7 cut(s) 602, 1047, 1065, 1202, 1379, 2142, 2148
GlaI GCGC 3 cut(s) 12, 71, 545
GsaI CCCAGC 3 cut(s) 126, 632, 1783
GsuI CTGGAG 1 cut(s) 1316
HaeIII GGCC 4 cut(s) 5, 29, 146, 2134
HapII CCGG 5 cut(s) 26, 30, 40, 199, 803
HgaI GACGC 1 cut(s) 17
HhaI GCGC 3 cut(s) 13, 72, 546
Hin6I GCGC 3 cut(s) 11, 70, 544
HinP1I GCGC 3 cut(s) 11, 70, 544
HincII GTYRAC 2 cut(s) 243, 822
HindII GTYRAC 2 cut(s) 243, 822
HindIII AAGCTT 1 cut(s) 416
HpaI GTTAAC 1 cut(s) 822
HpaII CCGG 5 cut(s) 26, 30, 40, 199, 803
HphI GGTGA 5 cut(s) 174, 188, 1539, 1859, 1943
Hpy166II GTNNAC 4 cut(s) 243, 822, 1357, 1371
Hpy8I GTNNAC 4 cut(s) 243, 822, 1357, 1371
Hpy99I CGWCG 2 cut(s) 11, 121
HpyCH4III ACNGT 6 cut(s) 19, 235, 598, 1368, 1606, 1825
HpyF3I CTNAG 6 cut(s) 258, 430, 536, 1581, 1703, 1923
HspAI GCGC 3 cut(s) 11, 70, 544
Kpn2I TCCGGA 2 cut(s) 39, 802
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Ksp22I TGATCA 1 cut(s) 150
KspAI GTTAAC 1 cut(s) 822
LguI GCTCTTC 1 cut(s) 960
LmnI GCTCC 5 cut(s) 42, 541, 899, 975, 1013
Lsp1109I GCAGC 9 cut(s) 121, 211, 214, 241, 439, 781, 2020, 2023, 2026
LweI GCATC 7 cut(s) 271, 394, 424, 1026, 1333, 1415, 2026
MaeI CTAG 7 cut(s) 602, 1047, 1065, 1202, 1379, 2142, 2148
MaeIII GTNAC 7 cut(s) 490, 1541, 1674, 1770, 1918, 1931, 2166
MbiI CCGCTC 1 cut(s) 83
MflI RGATCY 3 cut(s) 1171, 1471, 2144
MhlI GDGCHC 2 cut(s) 39, 972
MlyI GAGTC 5 cut(s) 422, 1320, 1504, 1804, 1882
MmeI TCCRAC 2 cut(s) 991, 1116
Mph1103I ATGCAT 2 cut(s) 1216, 1220
MroI TCCGGA 2 cut(s) 39, 802
MroNI GCCGGC 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 1867
MslI CAYNNNNRTG 1 cut(s) 1113
MspA1I CMGCKG 2 cut(s) 995, 1657
MspI CCGG 5 cut(s) 26, 30, 40, 199, 803
MspR9I CCNGG 4 cut(s) 47, 1397, 1725, 1943
Mva1269I GAATGC 1 cut(s) 82
MvaI CCWGG 4 cut(s) 47, 1397, 1725, 1943
MvnI CGCG 2 cut(s) 11, 954
NaeI GCCGGC 1 cut(s) 31
NcoI CCATGG 1 cut(s) 1551
NdeI CATATG 1 cut(s) 1734
NgoMIV GCCGGC 1 cut(s) 29
NlaIV GGNNCC 3 cut(s) 44, 866, 1722
NmuCI GTSAC 3 cut(s) 1541, 1918, 1931
NruI TCGCGA 1 cut(s) 954
NsiI ATGCAT 2 cut(s) 1216, 1220
NspI RCATGY 4 cut(s) 1218, 1598, 1903, 2030
PaeI GCATGC 3 cut(s) 1218, 1598, 2030
PaeR7I CTCGAG 1 cut(s) 86
PagI TCATGA 1 cut(s) 130
PceI AGGCCT 1 cut(s) 2134
PciI ACATGT 1 cut(s) 1899
PciSI GCTCTTC 1 cut(s) 960
PctI GAATGC 1 cut(s) 82
PdiI GCCGGC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 1867
PfeI GAWTC 8 cut(s) 22, 99, 193, 677, 707, 1388, 1609, 1669
PflMI CCANNNNNTGG 1 cut(s) 580
PleI GAGTC 5 cut(s) 422, 1320, 1503, 1804, 1882
PpsI GAGTC 5 cut(s) 422, 1320, 1503, 1804, 1882
PscI ACATGT 1 cut(s) 1899
PshBI ATTAAT 2 cut(s) 1635, 2055
PsiI TTATAA 2 cut(s) 1562, 2176
Psp124BI GAGCTC 1 cut(s) 972
Psp6I CCWGG 4 cut(s) 45, 1395, 1723, 1941
PspFI CCCAGC 3 cut(s) 122, 628, 1779
PspGI CCWGG 4 cut(s) 45, 1395, 1723, 1941
PspN4I GGNNCC 3 cut(s) 44, 866, 1722
PspPI GGNCC 1 cut(s) 865
PstNI CAGNNNCTG 2 cut(s) 1618, 1717
PsuI RGATCY 3 cut(s) 1171, 1471, 2144
PvuII CAGCTG 2 cut(s) 995, 1657
RruI TCGCGA 1 cut(s) 954
RsaI GTAC 5 cut(s) 366, 444, 1603, 1827, 1835
RsaNI GTAC 5 cut(s) 365, 443, 1602, 1826, 1834
RseI CAYNNNNRTG 1 cut(s) 1113
SacI GAGCTC 1 cut(s) 972
SapI GCTCTTC 1 cut(s) 960
Sau96I GGNCC 1 cut(s) 865
ScaI AGTACT 1 cut(s) 1603
SchI GAGTC 5 cut(s) 422, 1320, 1504, 1804, 1882
ScrFI CCNGG 4 cut(s) 47, 1397, 1725, 1943
SduI GDGCHC 2 cut(s) 39, 972
SfaNI GCATC 7 cut(s) 271, 394, 424, 1026, 1333, 1415, 2026
SfcI CTRYAG 2 cut(s) 753, 1988
Sfr274I CTCGAG 1 cut(s) 86
SinI GGWCC 1 cut(s) 865
SlaI CTCGAG 1 cut(s) 86
SmiMI CAYNNNNRTG 1 cut(s) 1113
SmlI CTYRAG 2 cut(s) 86, 266
SmoI CTYRAG 2 cut(s) 86, 266
SphI GCATGC 3 cut(s) 1218, 1598, 2030
SseBI AGGCCT 1 cut(s) 2134
SsiI CCGC 6 cut(s) 50, 81, 185, 571, 1243, 1791
SspMI CTAG 7 cut(s) 602, 1047, 1065, 1202, 1379, 2142, 2148
SstI GAGCTC 1 cut(s) 972
StuI AGGCCT 1 cut(s) 2134
StyD4I CCNGG 4 cut(s) 45, 1395, 1723, 1941
StyI CCWWGG 1 cut(s) 1551
TaaI ACNGT 6 cut(s) 19, 235, 598, 1368, 1606, 1825
TaqI TCGA 2 cut(s) 87, 469
TatI WGTACW 2 cut(s) 442, 1601
TauI GCSGC 2 cut(s) 83, 1793
TfiI GAWTC 8 cut(s) 22, 99, 193, 677, 707, 1388, 1609, 1669
TscAI CASTG 4 cut(s) 601, 1088, 1927, 1984
TseFI GTSAC 3 cut(s) 1541, 1918, 1931
TseI GCWGC 9 cut(s) 109, 224, 227, 254, 452, 794, 2008, 2011, 2014
Tsp45I GTSAC 3 cut(s) 1541, 1918, 1931
TspDTI ATGAA 8 cut(s) 563, 822, 978, 1199, 1256, 1524, 2106, 2141
TspGWI ACGGA 4 cut(s) 182, 962, 1265, 1822
TspRI CASTG 4 cut(s) 601, 1088, 1927, 1984
Van91I CCANNNNNTGG 1 cut(s) 580
VpaK11BI GGWCC 1 cut(s) 865
VspI ATTAAT 2 cut(s) 1635, 2055
XagI CCTNNNNNAGG 1 cut(s) 1157
XapI RAATTY 6 cut(s) 170, 521, 809, 1643, 1800, 1876
XbaI TCTAGA 3 cut(s) 601, 1201, 2141
XceI RCATGY 4 cut(s) 1218, 1598, 1903, 2030
XhoI CTCGAG 1 cut(s) 86
XmiI GTMKAC 2 cut(s) 1356, 1370
XmnI GAANNNNTTC 1 cut(s) 1867
XspI CTAG 7 cut(s) 602, 1047, 1065, 1202, 1379, 2142, 2148
ZrmI AGTACT 1 cut(s) 1603
Zsp2I ATGCAT 2 cut(s) 1216, 1220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.