MD16G1018900.v1.1

Protein VAC14 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012295
Physical Location & Seq
Forward (+)
1367703 .. 1374663
6961 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1018900.v1.1.491

Sequence Viewer

Length: 2184 bp
ATGGCCGACGCTCTCTCGGTGATTCCCGCGGGCGTCCTCCGCAACCTCGCGGACAAGCTCTACGAGAAGCGCAAGAATGCCGCTCTTGAGGTTGAGGGAATAGTGAAACAGCTCACGAATGCCGGGGATCACGATAAGATTACGGCTGTGATCAATTTGCTCACTACGGAATTCACTTACTCGCCGCAAGCGAATCACCGGAAGGGAGGATTGATAGGATTAGCTGCTGCAACAGTTGGGTTGACCATTGAAGCGGCTCAGCATCTTGAGCAAATTGTGCCACCTGTGCTTAATTCCTTTTCGGACCAAGATAGCAGAGTTCGTTATTATGCTTGTGAGGCGCTATATAACATTGCAAAGGTTGTAAGAGGAGATTTTATCATATTCTTTAATCAGATATTTGATGCCTTGTGTAAGCTTTCAACAGACTCGGATGCTAACGTACAAAGTGCTGCTCATCTTTTAGATCGGCTTGTAAAGGATATTGTTACTGAAAGTGATCAATTCAGCATTGAAGAATTTATTCCACTGTTGAGGGAGCGTATGAATGTCCTGAATCCCTATGTTCGTCAGTTTTTGGTTGGATGGATCACTGTCTTAGACAGTGTTCCAGATATTGACATGCTGGGATTTCTTCCTGATTTTCTTGATGGCTTGTTCAATATGTTAAGTGATTCAAGCCATGAGATTAGGCAACAAGCTGATTCAGCACTTTCAGAGTTTCTCCAAGAGATCAAGAACTCCCCATCTGTAGATTATGGTCGCATGGCTGAAATACTGGTACAGAGGGCTGCTTCACCGGATGAATTTACTCGGTTAACAGCTATCACATGGATAAATGAGTTTGTAAAACTTGGTGGGGACCAGCTTGTTCCCTATTATGCTGATATTCTGGGAGCGATTTTACCCTGCATATCTGATAAAGAAGAGAAAATTAGAGTGGTTGCTCGTGAAACCAATGAGGAGATTCGTGCCATTAAGGCTGATCCAGCTGAAGGGTTTGACGTTGGAGCAATCCTCTCTATTGCAAGGAGGCAATTATCTAGTGAATGGGAGGCTACTAGAATCGAAGCACTGCACTGGATTTCAAACCTTTTAAACAGACATCGCGCTGAGGTTCTAACTTTTCTGAACGACATATTTAACACCCTTTTGGAAGCGCTCTCAGATGCTTCTGATCAGGTGGTTCTCTTGGTCCTAGAAGTTCATGCATGCATAGCAAAAGATCCACAACACTTCCGCCAGCTTGTTGTGTTCCTTGTGCATAATTTTCGTGTGGATAACTCTCTTCTAGAAAAACGTGGTGCGTTGATTATTCGCCGACTTTGTGTGCTTTTAGATGCTGAAAGGGTCTACCGAGAACTGTCTACTATACTAGAAGCAGAGTCAGACCTGGATTTTGCATCTGTTATGGTTCAGGCTTTGAACTTGATTTTACTTACATCGTCCGAGTTGGCTGAGCTTCGAGATCTTTTGAAAAGATCACTTGTGAATCCTTCTGGGAAGGACTTATTTGTTTCCTTATATGCTTCATGGTGCCACTCACCCATGGCAATTATAAGCCTCTGCTTATTAGCTCAGACATATCAGCATGCGAGTACTGTGATTCAGTCTCTGGTGGAGGAAGATATTAATGTCAAGTTTTTGGTCCAGCTGGATAAATTGATTCGGTTACTAGAAACTCCAATCTTTGCGTATCTTAGATTACAGCTTCTTGAACCTGGAAGACATATGTGGTTGTTAAAAACCTTGTATGGTCTTCTGATGTTGCTTCCTCAGCAAAGTGCCGCCTTTAAAATTCTACGGACGCGTTTAAAAACTGTCCCTTCATACGCATTCAACAGTGAGCAACTCAGACGAACTTCTTCAGGGAATCCCCATCAGATTATACCTCACATGCCAAGTTGTTCGCAGATCATTGAAGACGGGGACATAAACCAGGACAGTAAGAGTTCTCATAATGGAATAAACTTTGCTTTAAGACTACAGCAGTTTGAGCAAATGCATCAGCAACATCGTCAGCATGCAAAAGTGCAGGCACAGTCACACAGTACTTCCACAGCTTCTTCAACACCGAAGGATGTGCAAAGACCTGAAGAACAAAGCCATAGCCGATCGCCTTCATCAGATGTGAATAGGCTTCCTTCCAGATCTCGGAGGGCCCCGGGGCAGTTACAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05166 ko05203 map05166 map05203
Pfam Domains
Protein Families
Vac14_Fab1_bd Vac14_Fig4_bd

Protein Analysis

728

Amino Acids

81.84

Weight (kDa)

5.7

Isoelectric Point (pI)

51.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOR1L1_N PF24714 15 - 240 2.1e-06 TORTIFOLIA1 N-terminal
Vac14_Fab1_bd PF12755 65 - 161 4.3e-40 Vacuolar 14 Fab1-binding region
HEAT_EF3_N PF24987 248 - 395 1.5e-08 EF3 N-terminal HEAT repeat
Vac14_Fig4_bd PF11916 429 - 608 1.2e-72 Vacuolar protein 14 C-terminal Fig4p binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012295)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01690 AT2G01690
fragaria_vesca FvH4_4g33570 FvH4_4g33570
malus_domestica MD13G1020500.v1.1 MD16G1018900.v1.1
prunus_persica Prupe.1G333200_v2.0.a1
pyrus_communis pycom13g01730 pycom16g01700
rosa_chinensis RchiOBHm_Chr4g0443071
rosa_laevigata RLG00000005956
rosa_multiflora Rmu_sc0008069.1_g000004
rosa_roxburghii Rroxscaffold_5G00383700
rosa_rugosa Rorug04G0344200 Rorug04G0344200
rosa_samantha Rh4BG410000 Rh4CG425500 Rh4DG404900
rosa_wichuraiana Rw4G034340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1559, 2182
AccB1I GGYRCC 1 cut(s) 1536
AccBSI CCGCTC 1 cut(s) 83
AccI GTMKAC 2 cut(s) 1353, 1367
AccII CGCG 4 cut(s) 29, 50, 1110, 1810
AciI CCGC 9 cut(s) 27, 29, 40, 50, 81, 185, 254, 1240, 1788
AclWI GGATC 4 cut(s) 135, 596, 980, 1220
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 4 cut(s) 170, 518, 806, 1797
AcuI CTGAAG 3 cut(s) 1014, 1851, 2115
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 4 cut(s) 444, 783, 1600, 2053
AfeI AGCGCT 1 cut(s) 1161
AfiI CCNNNNNNNGG 2 cut(s) 995, 2154
AflIII ACRYGT 1 cut(s) 1808
AjnI CCWGG 3 cut(s) 1392, 1720, 1938
Alw26I GTCTC 1 cut(s) 1617
AlwI GGATC 4 cut(s) 135, 596, 980, 1220
AlwNI CAGNNNCTG 1 cut(s) 1615
Ama87I CYCGRG 1 cut(s) 2164
Aor51HI AGCGCT 1 cut(s) 1161
AoxI GGCC 2 cut(s) 3, 2160
ApaI GGGCCC 1 cut(s) 2164
ApeKI GCWGC 4 cut(s) 224, 227, 452, 791
ApoI RAATTY 4 cut(s) 170, 518, 806, 1797
ArsI GACNNNNNNTTYG 2 cut(s) 1382, 1414
AseI ATTAAT 1 cut(s) 1632
Asp700I GAANNNNTTC 2 cut(s) 522, 1864
AspLEI GCGC 4 cut(s) 72, 343, 1112, 1162
AspS9I GGNCC 6 cut(s) 304, 862, 1195, 1648, 2160, 2161
AsuC2I CCSGG 3 cut(s) 124, 2165, 2166
AsuHPI GGTGA 4 cut(s) 31, 188, 789, 1536
AvaI CYCGRG 1 cut(s) 2164
AvaII GGWCC 4 cut(s) 304, 862, 1195, 1648
BaeGI GKGCMC 1 cut(s) 2164
BanI GGYRCC 1 cut(s) 1536
BanII GRGCYC 1 cut(s) 2164
BauI CACGAG 1 cut(s) 948
BbsI GAAGAC 3 cut(s) 1732, 1751, 1929
BbvCI CCTCAGC 2 cut(s) 1113, 1776
BbvI GCAGC 4 cut(s) 211, 214, 439, 778
BccI CCATC 4 cut(s) 579, 644, 754, 1887
BceAI ACGGC 1 cut(s) 159
BcgI CGANNNNNNTGC 4 cut(s) 1253, 1287, 2065, 2099
BciT130I CCWGG 3 cut(s) 1394, 1722, 1940
BclI TGATCA 3 cut(s) 150, 499, 1177
BcnI CCSGG 3 cut(s) 124, 2165, 2166
BcoDI GTCTC 1 cut(s) 1617
BfaI CTAG 6 cut(s) 1044, 1062, 1199, 1292, 1376, 1676
BfmI CTRYAG 2 cut(s) 750, 1985
BfoI RGCGCY 2 cut(s) 344, 1163
BglI GCCNNNNNGGC 1 cut(s) 980
BglII AGATCT 2 cut(s) 1468, 2150
BisI GCNGC 8 cut(s) 81, 185, 225, 228, 255, 453, 792, 1788
BlpI GCTNAGC 2 cut(s) 258, 1458
BlsI GCNGC 8 cut(s) 82, 186, 226, 229, 256, 454, 793, 1789
BmcAI AGTACT 2 cut(s) 1600, 2053
Bme1390I CCNGG 6 cut(s) 124, 1394, 1722, 1940, 2165, 2166
Bme18I GGWCC 4 cut(s) 304, 862, 1195, 1648
BmeT110I CYCGRG 1 cut(s) 2164
BmgT120I GGNCC 6 cut(s) 304, 862, 1195, 1648, 2160, 2161
BmiI GGNNCC 4 cut(s) 863, 1538, 2162, 2163
BmrFI CCNGG 6 cut(s) 124, 1394, 1722, 1940, 2165, 2166
BmsI GCATC 7 cut(s) 271, 394, 424, 1159, 1330, 1412, 2014
BpiI GAAGAC 3 cut(s) 1732, 1751, 1929
BplI GAGNNNNNCTC 2 cut(s) 1002, 1034
Bpu10I CCTNAGC 2 cut(s) 1113, 1776
Bpu1102I GCTNAGC 2 cut(s) 258, 1458
BpuEI CTTGAG 2 cut(s) 107, 287
BpuMI CCSGG 3 cut(s) 124, 2165, 2166
BsaHI GRCGYC 1 cut(s) 33
BsaJI CCNNGG 6 cut(s) 27, 123, 1548, 2163, 2164, 2165
BsaWI WCCGGW 2 cut(s) 198, 799
Bsc4I CCNNNNNNNGG 2 cut(s) 995, 2154
Bse1I ACTGG 2 cut(s) 783, 1085
Bse3DI GCAATG 1 cut(s) 351
BseBI CCWGG 3 cut(s) 1394, 1722, 1940
BseDI CCNNGG 6 cut(s) 27, 123, 1548, 2163, 2164, 2165
BseGI GGATG 4 cut(s) 439, 590, 808, 2086
BseLI CCNNNNNNNGG 2 cut(s) 995, 2154
BseMI GCAATG 1 cut(s) 351
BseMII CTCAG 7 cut(s) 272, 1104, 1179, 1449, 1592, 1790, 1867
BseNI ACTGG 2 cut(s) 783, 1085
BseRI GAGGAG 2 cut(s) 384, 977
BseSI GKGCMC 1 cut(s) 2164
BseXI GCAGC 4 cut(s) 211, 214, 439, 778
BseYI CCCAGC 1 cut(s) 625
BsgI GTGCAG 2 cut(s) 1061, 2054
Bsh1236I CGCG 4 cut(s) 29, 50, 1110, 1810
Bsh1285I CGRYCG 1 cut(s) 2117
BshFI GGCC 2 cut(s) 5, 2162
BshNI GGYRCC 1 cut(s) 1536
BsiEI CGRYCG 1 cut(s) 2117
BsiHKCI CYCGRG 1 cut(s) 2164
BsiSI CCGG 4 cut(s) 123, 199, 800, 2165
BslFI GGGAC 3 cut(s) 875, 1808, 1943
BslI CCNNNNNNNGG 2 cut(s) 995, 2154
BsmAI GTCTC 1 cut(s) 1617
BsmFI GGGAC 3 cut(s) 875, 1808, 1943
BsmI GAATGC 3 cut(s) 82, 124, 1835
BsnI GGCC 2 cut(s) 5, 2162
BsoBI CYCGRG 1 cut(s) 2164
Bsp120I GGGCCC 1 cut(s) 2160
Bsp1286I GDGCHC 1 cut(s) 2164
Bsp1720I GCTNAGC 2 cut(s) 258, 1458
Bsp19I CCATGG 1 cut(s) 1548
BspACI CCGC 9 cut(s) 27, 29, 40, 50, 81, 185, 254, 1240, 1788
BspANI GGCC 2 cut(s) 5, 2162
BspCNI CTCAG 7 cut(s) 271, 1105, 1178, 1450, 1591, 1789, 1866
BspFNI CGCG 4 cut(s) 29, 50, 1110, 1810
BspLI GGNNCC 4 cut(s) 863, 1538, 2162, 2163
BspPI GGATC 4 cut(s) 135, 596, 980, 1220
BspT107I GGYRCC 1 cut(s) 1536
BsrBI CCGCTC 1 cut(s) 83
BsrDI GCAATG 1 cut(s) 351
BsrI ACTGG 2 cut(s) 783, 1085
BssECI CCNNGG 6 cut(s) 27, 123, 1548, 2163, 2164, 2165
BssNI GRCGYC 1 cut(s) 33
BssSI CACGAG 1 cut(s) 948
BssT1I CCWWGG 1 cut(s) 1548
Bst2BI CACGAG 1 cut(s) 948
Bst2UI CCWGG 3 cut(s) 1394, 1722, 1940
Bst6I CTCTTC 2 cut(s) 921, 1293
BstACI GRCGYC 1 cut(s) 33
BstAPI GCANNNNNTGC 1 cut(s) 277
BstC8I GCNNGC 7 cut(s) 31, 189, 1213, 1244, 1593, 2025, 2037
BstDEI CTNAG 9 cut(s) 258, 598, 1113, 1165, 1458, 1578, 1700, 1776, 1853
BstDSI CCRYGG 2 cut(s) 27, 1548
BstF5I GGATG 4 cut(s) 439, 590, 808, 2086
BstFNI CGCG 4 cut(s) 29, 50, 1110, 1810
BstH2I RGCGCY 2 cut(s) 344, 1163
BstHHI GCGC 4 cut(s) 72, 343, 1112, 1162
BstMAI GTCTC 1 cut(s) 1617
BstMCI CGRYCG 1 cut(s) 2117
BstNI CCWGG 3 cut(s) 1394, 1722, 1940
BstNSI RCATGY 5 cut(s) 625, 1215, 1595, 1900, 2027
BstSCI CCNGG 6 cut(s) 122, 1392, 1720, 1938, 2163, 2164
BstSFI CTRYAG 2 cut(s) 750, 1985
BstSLI GKGCMC 1 cut(s) 2164
BstUI CGCG 4 cut(s) 29, 50, 1110, 1810
BstV1I GCAGC 4 cut(s) 211, 214, 439, 778
BstV2I GAAGAC 3 cut(s) 1732, 1751, 1929
BstX2I RGATCY 3 cut(s) 1225, 1468, 2150
BstYI RGATCY 3 cut(s) 1225, 1468, 2150
BsuRI GGCC 2 cut(s) 5, 2162
BtgI CCRYGG 2 cut(s) 27, 1548
BtgZI GCGATG 1 cut(s) 1091
BtsCI GGATG 4 cut(s) 439, 590, 808, 2086
BtsI GCAGTG 1 cut(s) 1073
BtsIMutI CAGTG 6 cut(s) 527, 591, 610, 1073, 1078, 1849
Cac8I GCNNGC 7 cut(s) 31, 189, 1213, 1244, 1593, 2025, 2037
CaiI CAGNNNCTG 1 cut(s) 1615
CfoI GCGC 4 cut(s) 72, 343, 1112, 1162
Cfr13I GGNCC 6 cut(s) 304, 862, 1195, 1648, 2160, 2161
Cfr42I CCGCGG 1 cut(s) 30
Cfr9I CCCGGG 1 cut(s) 2164
CseI GACGC 3 cut(s) 17, 22, 1816
Csp6I GTAC 4 cut(s) 443, 782, 1599, 2052
CviQI GTAC 4 cut(s) 443, 782, 1599, 2052
DdeI CTNAG 9 cut(s) 258, 598, 1113, 1165, 1458, 1578, 1700, 1776, 1853
DraI TTTAAA 3 cut(s) 1098, 1795, 1815
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 921, 1293
EarI CTCTTC 2 cut(s) 921, 1293
EciI GGCGGA 1 cut(s) 1229
Eco130I CCWWGG 1 cut(s) 1548
Eco24I GRGCYC 1 cut(s) 2164
Eco47I GGWCC 4 cut(s) 304, 862, 1195, 1648
Eco47III AGCGCT 1 cut(s) 1161
Eco57I CTGAAG 3 cut(s) 1014, 1851, 2115
Eco88I CYCGRG 1 cut(s) 2164
EcoO109I RGGNCCY 2 cut(s) 2160, 2161
EcoRI GAATTC 1 cut(s) 170
EcoRII CCWGG 3 cut(s) 1392, 1720, 1938
EcoT14I CCWWGG 1 cut(s) 1548
EcoT22I ATGCAT 3 cut(s) 1213, 1217, 2007
EcoT38I GRGCYC 1 cut(s) 2164
ErhI CCWWGG 1 cut(s) 1548
FaqI GGGAC 3 cut(s) 875, 1808, 1943
FauI CCCGC 2 cut(s) 22, 34
FauNDI CATATG 1 cut(s) 1731
FbaI TGATCA 3 cut(s) 150, 499, 1177
FblI GTMKAC 2 cut(s) 1353, 1367
Fnu4HI GCNGC 8 cut(s) 81, 185, 225, 228, 255, 453, 792, 1788
FokI GGATG 4 cut(s) 446, 597, 815, 2093
FriOI GRGCYC 1 cut(s) 2164
Fsp4HI GCNGC 8 cut(s) 81, 185, 225, 228, 255, 453, 792, 1788
FspBI CTAG 6 cut(s) 1044, 1062, 1199, 1292, 1376, 1676
GlaI GCGC 4 cut(s) 71, 342, 1111, 1161
GluI GCNGC 8 cut(s) 81, 185, 225, 228, 255, 453, 792, 1788
GsaI CCCAGC 1 cut(s) 629
HaeII RGCGCY 2 cut(s) 344, 1163
HaeIII GGCC 2 cut(s) 5, 2162
HapII CCGG 4 cut(s) 123, 199, 800, 2165
HgaI GACGC 3 cut(s) 17, 22, 1816
HhaI GCGC 4 cut(s) 72, 343, 1112, 1162
Hin1I GRCGYC 1 cut(s) 33
Hin6I GCGC 4 cut(s) 70, 341, 1110, 1160
HinP1I GCGC 4 cut(s) 70, 341, 1110, 1160
HincII GTYRAC 2 cut(s) 243, 819
HindII GTYRAC 2 cut(s) 243, 819
HindIII AAGCTT 1 cut(s) 416
HpaI GTTAAC 1 cut(s) 819
HpaII CCGG 4 cut(s) 123, 199, 800, 2165
HphI GGTGA 4 cut(s) 31, 188, 789, 1536
Hpy166II GTNNAC 4 cut(s) 243, 819, 1354, 1368
Hpy8I GTNNAC 4 cut(s) 243, 819, 1354, 1368
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 8 cut(s) 196, 989, 1498, 1506, 1836, 2071, 2130, 2154
HpyCH4IV ACGT 3 cut(s) 441, 1005, 1300
HpyF3I CTNAG 9 cut(s) 258, 598, 1113, 1165, 1458, 1578, 1700, 1776, 1853
HpySE526I ACGT 3 cut(s) 441, 1005, 1300
Hsp92I GRCGYC 1 cut(s) 33
HspAI GCGC 4 cut(s) 70, 341, 1110, 1160
Ksp22I TGATCA 3 cut(s) 150, 499, 1177
KspAI GTTAAC 1 cut(s) 819
KspI CCGCGG 1 cut(s) 30
LmnI GCTCC 3 cut(s) 538, 896, 1010
Lsp1109I GCAGC 4 cut(s) 211, 214, 439, 778
LweI GCATC 7 cut(s) 271, 394, 424, 1159, 1330, 1412, 2014
MaeI CTAG 6 cut(s) 1044, 1062, 1199, 1292, 1376, 1676
MaeII ACGT 3 cut(s) 441, 1005, 1300
MaeIII GTNAC 4 cut(s) 487, 1671, 2043, 2172
MbiI CCGCTC 1 cut(s) 83
MflI RGATCY 3 cut(s) 1225, 1468, 2150
MhlI GDGCHC 1 cut(s) 2164
MluI ACGCGT 1 cut(s) 1808
MlyI GAGTC 2 cut(s) 422, 1394
MmeI TCCRAC 2 cut(s) 562, 988
Mph1103I ATGCAT 3 cut(s) 1213, 1217, 2007
MroXI GAANNNNTTC 2 cut(s) 522, 1864
MspA1I CMGCKG 3 cut(s) 29, 992, 1654
MspI CCGG 4 cut(s) 123, 199, 800, 2165
MspR9I CCNGG 6 cut(s) 124, 1394, 1722, 1940, 2165, 2166
Mva1269I GAATGC 3 cut(s) 82, 124, 1835
MvaI CCWGG 3 cut(s) 1394, 1722, 1940
MvnI CGCG 4 cut(s) 29, 50, 1110, 1810
NciI CCSGG 3 cut(s) 124, 2165, 2166
NcoI CCATGG 1 cut(s) 1548
NdeI CATATG 1 cut(s) 1731
NlaIV GGNNCC 4 cut(s) 863, 1538, 2162, 2163
NmuCI GTSAC 1 cut(s) 2043
NsiI ATGCAT 3 cut(s) 1213, 1217, 2007
NspI RCATGY 5 cut(s) 625, 1215, 1595, 1900, 2027
PaeI GCATGC 3 cut(s) 1215, 1595, 2027
PcsI WCGNNNNNNNCGW 1 cut(s) 188
PctI GAATGC 3 cut(s) 82, 124, 1835
PdmI GAANNNNTTC 2 cut(s) 522, 1864
PkrI GCNGC 8 cut(s) 82, 186, 226, 229, 256, 454, 793, 1789
Ple19I CGATCG 1 cut(s) 2117
PleI GAGTC 2 cut(s) 422, 1393
PpsI GAGTC 2 cut(s) 422, 1393
PshBI ATTAAT 1 cut(s) 1632
PsiI TTATAA 2 cut(s) 1559, 2182
Psp6I CCWGG 3 cut(s) 1392, 1720, 1938
PspFI CCCAGC 1 cut(s) 625
PspGI CCWGG 3 cut(s) 1392, 1720, 1938
PspN4I GGNNCC 4 cut(s) 863, 1538, 2162, 2163
PspOMI GGGCCC 1 cut(s) 2160
PspPI GGNCC 6 cut(s) 304, 862, 1195, 1648, 2160, 2161
PstNI CAGNNNCTG 1 cut(s) 1615
PsuI RGATCY 3 cut(s) 1225, 1468, 2150
PvuI CGATCG 1 cut(s) 2117
PvuII CAGCTG 2 cut(s) 992, 1654
RsaI GTAC 4 cut(s) 444, 783, 1600, 2053
RsaNI GTAC 4 cut(s) 443, 782, 1599, 2052
SacII CCGCGG 1 cut(s) 30
SatI GCNGC 8 cut(s) 81, 185, 225, 228, 255, 453, 792, 1788
Sau96I GGNCC 6 cut(s) 304, 862, 1195, 1648, 2160, 2161
ScaI AGTACT 2 cut(s) 1600, 2053
SchI GAGTC 2 cut(s) 422, 1394
ScrFI CCNGG 6 cut(s) 124, 1394, 1722, 1940, 2165, 2166
SduI GDGCHC 1 cut(s) 2164
SfaNI GCATC 7 cut(s) 271, 394, 424, 1159, 1330, 1412, 2014
SfcI CTRYAG 2 cut(s) 750, 1985
Sfr303I CCGCGG 1 cut(s) 30
SgrBI CCGCGG 1 cut(s) 30
SinI GGWCC 4 cut(s) 304, 862, 1195, 1648
SmaI CCCGGG 1 cut(s) 2166
SmlI CTYRAG 2 cut(s) 86, 266
SmoI CTYRAG 2 cut(s) 86, 266
SphI GCATGC 3 cut(s) 1215, 1595, 2027
SsiI CCGC 9 cut(s) 27, 29, 40, 50, 81, 185, 254, 1240, 1788
SspMI CTAG 6 cut(s) 1044, 1062, 1199, 1292, 1376, 1676
StyD4I CCNGG 6 cut(s) 122, 1392, 1720, 1938, 2163, 2164
StyI CCWWGG 1 cut(s) 1548
TaiI ACGT 3 cut(s) 444, 1008, 1303
TaqI TCGA 2 cut(s) 1068, 1465
TatI WGTACW 2 cut(s) 1598, 2051
TauI GCSGC 4 cut(s) 83, 187, 257, 1790
TscAI CASTG 6 cut(s) 534, 598, 610, 1080, 1085, 1849
TseFI GTSAC 1 cut(s) 2043
TseI GCWGC 4 cut(s) 224, 227, 452, 791
Tsp45I GTSAC 1 cut(s) 2043
TspDTI ATGAA 6 cut(s) 560, 819, 1196, 1521, 1818, 2112
TspGWI ACGGA 2 cut(s) 182, 1819
TspMI CCCGGG 1 cut(s) 2164
TspRI CASTG 6 cut(s) 534, 598, 610, 1080, 1085, 1849
VpaK11BI GGWCC 4 cut(s) 304, 862, 1195, 1648
VspI ATTAAT 1 cut(s) 1632
XapI RAATTY 4 cut(s) 170, 518, 806, 1797
XbaI TCTAGA 1 cut(s) 1291
XceI RCATGY 5 cut(s) 625, 1215, 1595, 1900, 2027
XmaI CCCGGG 1 cut(s) 2164
XmiI GTMKAC 2 cut(s) 1353, 1367
XmnI GAANNNNTTC 2 cut(s) 522, 1864
XspI CTAG 6 cut(s) 1044, 1062, 1199, 1292, 1376, 1676
ZrmI AGTACT 2 cut(s) 1600, 2053
Zsp2I ATGCAT 3 cut(s) 1213, 1217, 2007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.